Affiliation: University of Cambridge
Boroviak T, Nichols J. The birth of embryonic pluripotency. Philos Trans R Soc Lond B Biol Sci. 2014;369: pubmed publisher
..This property is lost after implantation as the epiblast epithelializes and becomes primed in preparation for gastrulation and subsequent organogenesis. ..
Nichols J, Boroviak T. Maximizing Clonal Embryonic Stem Cell Derivation by ERK Pathway Inhibition. Methods Mol Biol. 2016;1341:1-13 pubmed publisher
..Here, we provide details of the procedure for both maximizing the number of cells in the donor tissue and subsequent effective derivation of multiple clonal ES cell lines. ..
Mulas C, Chia G, Jones K, Hodgson A, Stirparo G, Nichols J. Oct4 regulates the embryonic axis and coordinates exit from pluripotency and germ layer specification in the mouse embryo. Development. 2018;145: pubmed publisher
Ramos Ibeas P, Nichols J, Alberio R. States and Origins of Mammalian Embryonic Pluripotency In Vivo and in a Dish. Curr Top Dev Biol. 2018;128:151-179 pubmed publisher
..Here, we discuss the recent developments in our understanding of early embryogenesis in different mammalian species. ..
Guo G, von Meyenn F, Santos F, Chen Y, Reik W, Bertone P, et al
. Naive Pluripotent Stem Cells Derived Directly from Isolated Cells of the Human Inner Cell Mass. Stem Cell Reports. 2016;6:437-446 pubmed publisher
..Collectively these attributes suggest classification as human naive embryonic stem cells. Human counterparts of canonical mouse embryonic stem cells would argue for conservation in the phased progression of pluripotency in mammals. ..
Nichols J, Silva J, Roode M, Smith A. Suppression of Erk signalling promotes ground state pluripotency in the mouse embryo. Development. 2009;136:3215-22 pubmed publisher
..We propose that ES cells are not a tissue culture creation, but are essentially identical to pre-implantation epiblast cells. ..
Nichols J, Smith A. The origin and identity of embryonic stem cells. Development. 2011;138:3-8 pubmed publisher
..Based on recent findings, we propose here that ES cells can be derived directly from early epiblast cells and that ES cells might arise via two different routes that are dictated by their culture conditions...
Alexandrova S, Kalkan T, Humphreys P, Riddell A, Scognamiglio R, Trumpp A, et al
. Selection and dynamics of embryonic stem cell integration into early mouse embryos. Development. 2016;143:24-34 pubmed publisher
..Upregulation of Rex1 occurred in a proportion of these cells, reflecting the potential of the embryonic environment to expedite diversion from differentiation priming to enhance the developing embryonic epiblast. ..
Mohammed H, Hernando Herraez I, Savino A, Scialdone A, Macaulay I, Mulas C, et al
. Single-Cell Landscape of Transcriptional Heterogeneity and Cell Fate Decisions during Mouse Early Gastrulation. Cell Rep. 2017;20:1215-1228 pubmed publisher
..Our study systematically charts transcriptional noise and uncovers molecular processes associated with early lineage decisions. ..