Michael P Murphy

Summary

Affiliation: University of Cambridge
Country: UK

Publications

  1. doi request reprint Unraveling the biological roles of reactive oxygen species
    Michael P Murphy
    MRC Mitochondrial Biology Unit, Cambridge, UK
    Cell Metab 13:361-6. 2011
  2. pmc Mitochondria-targeted antioxidant MitoQ ameliorates experimental mouse colitis by suppressing NLRP3 inflammasome-mediated inflammatory cytokines
    Amarjargal Dashdorj
    Department of Biochemistry and Molecular Biology, School of Medicine, Kyung Hee University, Seoul 130 701, Republic of Korea
    BMC Med 11:178. 2013
  3. doi request reprint Mitochondrial dysfunction indirectly elevates ROS production by the endoplasmic reticulum
    Michael P Murphy
    MRC Mitochondrial Biology Unit, Cambridge, UK
    Cell Metab 18:145-6. 2013
  4. doi request reprint Mitochondrial thiols in antioxidant protection and redox signaling: distinct roles for glutathionylation and other thiol modifications
    Michael P Murphy
    MRC Mitochondrial Biology Unit, Cambridge, United Kingdom
    Antioxid Redox Signal 16:476-95. 2012
  5. ncbi request reprint Interactions of mitochondria-targeted and untargeted ubiquinones with the mitochondrial respiratory chain and reactive oxygen species. Implications for the use of exogenous ubiquinones as therapies and experimental tools
    Andrew M James
    Medical Research Council Dunn Human Nutrition Unit, Wellcome Trust MRC Building, Hills Road, Cambridge CB2 2XY, United Kingdom
    J Biol Chem 280:21295-312. 2005
  6. doi request reprint The mitochondria-targeted antioxidant MitoQ decreases features of the metabolic syndrome in ATM+/-/ApoE-/- mice
    John R Mercer
    Division of Cardiovascular Medicine, University of Cambridge, Addenbrooke s Hospital, Cambridge, UK
    Free Radic Biol Med 52:841-9. 2012
  7. doi request reprint Rapid and extensive uptake and activation of hydrophobic triphenylphosphonium cations within cells
    Meredith F Ross
    Medical Research Council Dunn Human Nutrition Unit, Wellcome Trust MRC Building, Hills Road, Cambridge CB2 0XY, UK
    Biochem J 411:633-45. 2008
  8. doi request reprint A mitochondria-targeted nitroxide is reduced to its hydroxylamine by ubiquinol in mitochondria
    Jan Trnka
    Medical Research Council Dunn Human Nutrition Unit, Wellcome Trust MRC Building, Hills Road, Cambridge CB2 0XY, UK
    Free Radic Biol Med 44:1406-19. 2008
  9. ncbi request reprint Mitochondrial targeting of quinones: therapeutic implications
    Helena M Cocheme
    MRC Dunn Human Nutrition Unit, Wellcome Trust MRC Building, Hills Road, Cambridge CB2 2XY, UK
    Mitochondrion 7:S94-102. 2007
  10. doi request reprint Quantification and identification of mitochondrial proteins containing vicinal dithiols
    Raquel Requejo
    MRC Mitochondrial Biology Unit, Cambridge, UK
    Arch Biochem Biophys 504:228-35. 2010

Collaborators

Detail Information

Publications63

  1. doi request reprint Unraveling the biological roles of reactive oxygen species
    Michael P Murphy
    MRC Mitochondrial Biology Unit, Cambridge, UK
    Cell Metab 13:361-6. 2011
    ..Considerable progress is being made in addressing these issues, and here we survey some recent developments for those contemplating research in this area...
  2. pmc Mitochondria-targeted antioxidant MitoQ ameliorates experimental mouse colitis by suppressing NLRP3 inflammasome-mediated inflammatory cytokines
    Amarjargal Dashdorj
    Department of Biochemistry and Molecular Biology, School of Medicine, Kyung Hee University, Seoul 130 701, Republic of Korea
    BMC Med 11:178. 2013
    ..In this study, we assessed the therapeutic effects of MitoQ in a mouse model of experimental colitis and investigated the possible mechanisms underlying its effects on intestinal inflammation...
  3. doi request reprint Mitochondrial dysfunction indirectly elevates ROS production by the endoplasmic reticulum
    Michael P Murphy
    MRC Mitochondrial Biology Unit, Cambridge, UK
    Cell Metab 18:145-6. 2013
    ..Leadsham et al. (2013) now show that the link between mitochondrial damage and ROS is more complicated, at least in yeast, where signals from damaged mitochondria increase ROS production from the endoplasmic reticulum surface...
  4. doi request reprint Mitochondrial thiols in antioxidant protection and redox signaling: distinct roles for glutathionylation and other thiol modifications
    Michael P Murphy
    MRC Mitochondrial Biology Unit, Cambridge, United Kingdom
    Antioxid Redox Signal 16:476-95. 2012
    ..In addition, the changes in the redox state of many components of these mitochondrial thiol systems may transduce and relay redox signals within and through the mitochondrial matrix to modulate the activity of biochemical processes...
  5. ncbi request reprint Interactions of mitochondria-targeted and untargeted ubiquinones with the mitochondrial respiratory chain and reactive oxygen species. Implications for the use of exogenous ubiquinones as therapies and experimental tools
    Andrew M James
    Medical Research Council Dunn Human Nutrition Unit, Wellcome Trust MRC Building, Hills Road, Cambridge CB2 2XY, United Kingdom
    J Biol Chem 280:21295-312. 2005
    ..Our results comprehensively describe the interaction of exogenous ubiquinones with mitochondria and have implications for their rational design and use as therapies and as research tools to probe mitochondrial function...
  6. doi request reprint The mitochondria-targeted antioxidant MitoQ decreases features of the metabolic syndrome in ATM+/-/ApoE-/- mice
    John R Mercer
    Division of Cardiovascular Medicine, University of Cambridge, Addenbrooke s Hospital, Cambridge, UK
    Free Radic Biol Med 52:841-9. 2012
    ..These findings strengthen the growing view that elevated mitochondrial ROS contributes to the etiology of the metabolic syndrome and suggest a potential therapeutic role for mitochondria-targeted antioxidants...
  7. doi request reprint Rapid and extensive uptake and activation of hydrophobic triphenylphosphonium cations within cells
    Meredith F Ross
    Medical Research Council Dunn Human Nutrition Unit, Wellcome Trust MRC Building, Hills Road, Cambridge CB2 0XY, UK
    Biochem J 411:633-45. 2008
    ..The strong influence of hydrophobicity on TPP cation uptake into mitochondria within cells facilitates the rational design of mitochondria-targeted compounds to report on and modify mitochondrial function in vivo...
  8. doi request reprint A mitochondria-targeted nitroxide is reduced to its hydroxylamine by ubiquinol in mitochondria
    Jan Trnka
    Medical Research Council Dunn Human Nutrition Unit, Wellcome Trust MRC Building, Hills Road, Cambridge CB2 0XY, UK
    Free Radic Biol Med 44:1406-19. 2008
    ....
  9. ncbi request reprint Mitochondrial targeting of quinones: therapeutic implications
    Helena M Cocheme
    MRC Dunn Human Nutrition Unit, Wellcome Trust MRC Building, Hills Road, Cambridge CB2 2XY, UK
    Mitochondrion 7:S94-102. 2007
    ..MitoQ protects mitochondria against oxidative damage in vitro and following oral delivery, and may therefore form the basis for mitochondria-protective therapies...
  10. doi request reprint Quantification and identification of mitochondrial proteins containing vicinal dithiols
    Raquel Requejo
    MRC Mitochondrial Biology Unit, Cambridge, UK
    Arch Biochem Biophys 504:228-35. 2010
    ..These findings are consistent with roles for mitochondrial vicinal dithiol proteins in antioxidant defence and redox signalling and these methodologies will enable these roles to be explored...
  11. pmc Accumulation of lipophilic dications by mitochondria and cells
    Meredith F Ross
    Medical Research Council Dunn Human Nutrition Unit, Wellcome Trust MRC Building, Hills Road, Cambridge CB2 2XY, UK
    Biochem J 400:199-208. 2006
    ..Instead, this suggests that it may be possible to form dications within mitochondria that then remain within the cell...
  12. ncbi request reprint Glutaredoxin 2 catalyzes the reversible oxidation and glutathionylation of mitochondrial membrane thiol proteins: implications for mitochondrial redox regulation and antioxidant DEFENSE
    Samantha M Beer
    Medical Research Council Dunn Human Nutrition Unit, Wellcome Trust MRC Building, Hills Road, Cambridge CB2 2XY, United Kingdom
    J Biol Chem 279:47939-51. 2004
    ..Our findings indicate that Grx2 plays a central role in the response of mitochondria to both redox signals and oxidative stress by facilitating the interplay between the mitochondrial glutathione pool and protein thiols...
  13. ncbi request reprint Targeting lipoic acid to mitochondria: synthesis and characterization of a triphenylphosphonium-conjugated alpha-lipoyl derivative
    Stephanie E Brown
    Medical Research Council Dunn Human Nutrition Unit, Wellcome Trust MRC Building, Hills Road, Cambridge CB2 2XY, UK
    Free Radic Biol Med 42:1766-80. 2007
    ..These results suggest that the protective action of LA in vivo may require its reduction to LAH(2) and that this reduction is largely mediated by TrxR...
  14. pmc Complex I within oxidatively stressed bovine heart mitochondria is glutathionylated on Cys-531 and Cys-704 of the 75-kDa subunit: potential role of CYS residues in decreasing oxidative damage
    Thomas R Hurd
    Medical Research Council Dunn Human Nutrition Unit, Wellcome Trust MRC Building, Hills Road, Cambridge CB2 0XY, United Kingdom
    J Biol Chem 283:24801-15. 2008
    ....
  15. ncbi request reprint Persistent S-nitrosation of complex I and other mitochondrial membrane proteins by S-nitrosothiols but not nitric oxide or peroxynitrite: implications for the interaction of nitric oxide with mitochondria
    Christina C Dahm
    Medical Research Council Dunn Human Nutrition Unit, Wellcome Trust Medical Research Council Building, Hills Road, Cambridge CB2 2XY, United Kingdom
    J Biol Chem 281:10056-65. 2006
    ..These findings point to a significant role for complex I S-nitrosation and consequent dysfunction during nitrosative stress in disorders such as Parkinson disease and sepsis...
  16. pmc Inactivation of pyruvate dehydrogenase kinase 2 by mitochondrial reactive oxygen species
    Thomas R Hurd
    Mitochondrial Biology Unit, Medical Research Council, Cambridge CB2 0XY, United Kingdom
    J Biol Chem 287:35153-60. 2012
    ..production, such as may occur under nutrient excess and low ATP demand, the increase in O(2)() and H(2)O(2) may provide feedback signals to modulate mitochondrial metabolism...
  17. doi request reprint Cardioprotection by S-nitrosation of a cysteine switch on mitochondrial complex I
    Edward T Chouchani
    Medical Research Council Mitochondrial Biology Unit, Cambridge, UK
    Nat Med 19:753-9. 2013
    ....
  18. ncbi request reprint Mitochondria-targeted redox probes as tools in the study of oxidative damage and ageing
    Andrew M James
    Medical Research Council Dunn, Hills Road, Cambridge CB2 2XY, UK
    Mech Ageing Dev 126:982-6. 2005
    ..Here, we outline how the formation and consequences of mitochondrial ROS production can be investigated using these probes...
  19. doi request reprint Using exomarkers to assess mitochondrial reactive species in vivo
    Angela Logan
    MRC Mitochondrial Biology Unit, Hills Road, Cambridge CB2 0XY, UK
    Biochim Biophys Acta 1840:923-30. 2014
    ..While a range of methods can be applied to in vitro systems, measuring the levels and relative changes in reactive species in vivo is challenging...
  20. pmc Identification of S-nitrosated mitochondrial proteins by S-nitrosothiol difference in gel electrophoresis (SNO-DIGE): implications for the regulation of mitochondrial function by reversible S-nitrosation
    Edward T Chouchani
    MRC Mitochondrial Biology Unit, Cambridge, UK
    Biochem J 430:49-59. 2010
    ..More generally, the development of SNO-DIGE facilitates robust investigation of protein S-nitrosation across the proteome...
  21. doi request reprint Measurement of H2O2 within living Drosophila during aging using a ratiometric mass spectrometry probe targeted to the mitochondrial matrix
    Helena M Cocheme
    Medical Research Council Mitochondrial Biology Unit, Hills Road, Cambridge CB2 0XY, UK
    Cell Metab 13:340-50. 2011
    ..Our findings provide approaches to investigate mitochondrial ROS in vivo and suggest that while an increase in overall mitochondrial H(2)O(2) correlates with aging, it may not be causative...
  22. ncbi request reprint Interaction of the mitochondria-targeted antioxidant MitoQ with phospholipid bilayers and ubiquinone oxidoreductases
    Andrew M James
    Medical Research Council Dunn Human Nutrition Unit, Wellcome Trust MRC Building, Hills Road, Cambridge CB2 2XY, United Kingdom
    J Biol Chem 282:14708-18. 2007
    ..These findings will facilitate the rational design of future mitochondria-targeted molecules...
  23. ncbi request reprint Superoxide activates uncoupling proteins by generating carbon-centered radicals and initiating lipid peroxidation: studies using a mitochondria-targeted spin trap derived from alpha-phenyl-N-tert-butylnitrone
    Michael P Murphy
    Medical Research Council Dunn Human Nutrition Unit, Wellcome Trust MRC Building, Hills Road, Cambridge CB2 2XY, United Kingdom
    J Biol Chem 278:48534-45. 2003
    ..Superoxide releases iron from iron-sulfur center proteins, which then generates carbon-centered radicals that initiate lipid peroxidation, yielding breakdown products that activate UCPs...
  24. ncbi request reprint Fine-tuning the hydrophobicity of a mitochondria-targeted antioxidant
    Jordi Asin-Cayuela
    Medical Research Council Dunn Human Nutrition Unit, Wellcome Trust MRC Building, Hills Road, Cambridge CB2 2XY, UK
    FEBS Lett 571:9-16. 2004
    ..This indicates how the uptake and distribution of mitochondria-targeted compounds within mitochondria and cells can be controlled, thereby facilitating investigations of mitochondrial oxidative damage...
  25. pmc A mitochondria-targeted S-nitrosothiol modulates respiration, nitrosates thiols, and protects against ischemia-reperfusion injury
    Tracy A Prime
    MRC Mitochondrial Biology Unit, Hills Road, Cambridge CB2 0XY, United Kingdom
    Proc Natl Acad Sci U S A 106:10764-9. 2009
    ..These results support the idea that selectively targeting NO(*) donors to mitochondria is an effective strategy to reversibly modulate respiration and to protect mitochondria against ischemia-reperfusion injury...
  26. doi request reprint Using the mitochondria-targeted ratiometric mass spectrometry probe MitoB to measure H2O2 in living Drosophila
    Helena M Cocheme
    Medical Research Council, Mitochondrial Biology Unit, Cambridge, UK
    Nat Protoc 7:946-58. 2012
    ..This method facilitates the investigation of mitochondrial H(2)O(2) in fly models of pathology and metabolic alteration, and it can also be extended to assess mitochondrial H(2)O(2) production in mouse and cell culture studies...
  27. pmc Proteomic approaches to the characterization of protein thiol modification
    Edward T Chouchani
    MRC Mitochondrial Biology Unit, Hills Road, Cambridge CB2 0XY, United Kingdom
    Curr Opin Chem Biol 15:120-8. 2011
    ..Here, we discuss a number of these approaches and their application to the identification of a variety of cysteine-centered redox modifications...
  28. pmc Cysteine residues exposed on protein surfaces are the dominant intramitochondrial thiol and may protect against oxidative damage
    Raquel Requejo
    MRC Mitochondrial Biology Unit, Wellcome Trust MRC Building, Cambridge, UK
    FEBS J 277:1465-80. 2010
    ..Therefore, exposed protein thiols are the dominant free thiol within the cell and may play a critical role in intracellular antioxidant defences against oxidative damage...
  29. doi request reprint Measuring mitochondrial protein thiol redox state
    Raquel Requejo
    Medical Research Council Mitochondrial Biology Unit, Wellcome Trust MRC Building, Cambridge, UK
    Methods Enzymol 474:123-47. 2010
    ..Here we outline some of the approaches that can be used to accomplish these goals and thereby infer the multiple roles of mammalian mitochondrial protein thiols in antioxidant defense and redox signaling...
  30. ncbi request reprint Glutathionylation of mitochondrial proteins
    Thomas R Hurd
    MRC Dunn Human Nutrition Unit, Cambridge, UK
    Antioxid Redox Signal 7:999-1010. 2005
    ..Here we review the mechanisms and physiological significance of the glutathionylation of mitochondrial thiol proteins...
  31. doi request reprint A ratiometric fluorescent probe for assessing mitochondrial phospholipid peroxidation within living cells
    Tracy A Prime
    MRC Mitochondrial Biology Unit, Wellcome Trust MRC Building, Cambridge, UK
    Free Radic Biol Med 53:544-53. 2012
    ....
  32. pmc Development of a single-chain, quasi-dimeric zinc-finger nuclease for the selective degradation of mutated human mitochondrial DNA
    Michal Minczuk
    MRC Laboratory of Molecular Biology, Hills Road, Cambridge CB2 2QH, UK
    Nucleic Acids Res 36:3926-38. 2008
    ..Therefore, mitochondria-targeted single-chain ZFNs are a promising candidate approach for the treatment of mtDNA diseases...
  33. ncbi request reprint Complex I is the major site of mitochondrial superoxide production by paraquat
    Helena M Cocheme
    Medical Research Council Dunn Human Nutrition Unit, Wellcome Trust MRC Building, Hills Road, Cambridge CB2 0XY, United Kingdom
    J Biol Chem 283:1786-98. 2008
    ..Together this membrane potential-dependent uptake across the mitochondrial inner membrane and the subsequent rapid reduction to the paraquat radical cation explain the toxicity of paraquat to mitochondria...
  34. pmc Cell-penetrating peptides do not cross mitochondrial membranes even when conjugated to a lipophilic cation: evidence against direct passage through phospholipid bilayers
    Meredith F Ross
    MRC Dunn Human Nutrition Unit, Wellcome Trust MRC Building, Hills Road, Cambridge CB2 2XY, UK
    Biochem J 383:457-68. 2004
    ....
  35. doi request reprint Chapter 19 Measuring redox changes to mitochondrial protein thiols with redox difference gel electrophoresis (redox-DIGE)
    Thomas R Hurd
    The Medical Research Council Mitochondrial Biology Unit, Cambridge, United Kingdom
    Methods Enzymol 456:343-61. 2009
    ..Importantly, this method is not only applicable to studies in isolated mitochondria but can also be applied to more complicated systems such as intact cells and perhaps even whole organisms...
  36. doi request reprint Complementation of coenzyme Q-deficient yeast by coenzyme Q analogues requires the isoprenoid side chain
    Andrew M James
    Wellcome Trust MRC Building, Cambridge, UK
    FEBS J 277:2067-82. 2010
    ..Using a DeltaCoQ double-knockout library we identified a number of genes that decrease the ability of yeast to grow on exogenous CoQ. Here we suggest that CoQ or its redox state may be a signal for growth during the shift to respiration...
  37. ncbi request reprint Detection of reactive oxygen species-sensitive thiol proteins by redox difference gel electrophoresis: implications for mitochondrial redox signaling
    Thomas R Hurd
    Medical Research Council Dunn Human Nutrition Unit, Wellcome Trust Medical Research Council Building, Hills Road, Cambridge, UK
    J Biol Chem 282:22040-51. 2007
    ..Thiol redox modification affected enzyme activity, suggesting that the reversible modification of enzyme activity by ROS from the respiratory chain may be an important and unexplored mode of mitochondrial redox signaling...
  38. doi request reprint Mitochondrial DNA damage can promote atherosclerosis independently of reactive oxygen species through effects on smooth muscle cells and monocytes and correlates with higher-risk plaques in humans
    Emma Yu
    Division of Cardiovascular Medicine, University of Cambridge, Addenbrooke s Centre for Clinical Investigation, P O Box 110, Addenbrooke s Hospital, Cambridge, CB2 2QQ, UK
    Circulation 128:702-12. 2013
    ..However, it is unclear whether mtDNA damage directly promotes atherogenesis or is a consequence of tissue damage, which cell types are involved, and whether its effects are mediated only through reactive oxygen species...
  39. pmc Antioxidant properties of MitoTEMPOL and its hydroxylamine
    Jan Trnka
    MRC Dunn Human Nutrition Unit, Cambridge, UK
    Free Radic Res 43:4-12. 2009
    ..Furthermore, as TEMPOL is also reduced to a hydroxylamine in vivo, many of its antioxidant effects may also be mediated by its hydroxylamine...
  40. pmc Antioxidants can inhibit basal autophagy and enhance neurodegeneration in models of polyglutamine disease
    Benjamin R Underwood
    Department of Medical Genetics, University of Cambridge, Cambridge Institute for Medical Research, Addenbrooke s Hospital, Hills Road, Cambridge CB2 0XY, UK
    Hum Mol Genet 19:3413-29. 2010
    ..Thus, the potential benefits in neurodegenerative diseases of some classes of antioxidants may be compromised by their autophagy-blocking properties...
  41. doi request reprint Targeting lipophilic cations to mitochondria
    Michael P Murphy
    MRC Dunn Human Nutrition Unit, Wellcome Trust MRC Building, Hills Road, Cambridge CB2 0XY, United Kingdom
    Biochim Biophys Acta 1777:1028-31. 2008
    ..Here we outline some of the background to the development of these compounds...
  42. ncbi request reprint Antioxidant and prooxidant properties of mitochondrial Coenzyme Q
    Andrew M James
    Medical Research Council Dunn Human Nutrition Unit, Cambridge, UK
    Arch Biochem Biophys 423:47-56. 2004
    ..To help understand how Coenzyme Q contributes to both mitochondrial oxidative damage and antioxidant defences, we have reviewed its antioxidant and prooxidant properties...
  43. doi request reprint Mitochondrial oxidative stress and the metabolic syndrome
    Andrew M James
    Medical Research Council Mitochondrial Biology Unit, Wellcome Trust MRC Building, Hills Road, Cambridge CB2 0XY, UK
    Trends Endocrinol Metab 23:429-34. 2012
    ..From this we suggest a unifying hypothesis to integrate what is known about mitochondrial involvement in the metabolic syndrome that points to testable hypotheses and novel therapeutic approaches...
  44. pmc Mitochondrial uncouplers with an extraordinary dynamic range
    Phing How Lou
    MRC Dunn Human Nutrition Unit, Hills Road, Cambridge CB2 2XY, UK
    Biochem J 407:129-40. 2007
    ....
  45. pmc How mitochondria produce reactive oxygen species
    Michael P Murphy
    MRC Dunn Human Nutrition Unit, Hills Road, Cambridge CB20XY, UK
    Biochem J 417:1-13. 2009
    ....
  46. doi request reprint Mitochondrial accumulation of a lipophilic cation conjugated to an ionisable group depends on membrane potential, pH gradient and pK(a): implications for the design of mitochondrial probes and therapies
    Peter G Finichiu
    MRC Mitochondrial Biology Unit, Wellcome Trust MRC Building, Cambridge CB2 0XY, UK
    J Bioenerg Biomembr 45:165-73. 2013
    ..These findings may facilitate the rational design of additional mitochondrial targeted probes and therapies...
  47. ncbi request reprint Prevention of mitochondrial oxidative damage as a therapeutic strategy in diabetes
    Katherine Green
    Medical Research Council Dunn Human Nutrition Unit, Cambridge, UK
    Diabetes 53:S110-8. 2004
    ..Here, we outline the background to these strategies and discuss how antioxidants targeted to mitochondria, or selective mitochondrial uncoupling, may be potential therapies for diabetes...
  48. ncbi request reprint Targeting antioxidants to mitochondria by conjugation to lipophilic cations
    Michael P Murphy
    MRC Dunn Human Nutrition Unit, Wellcome Trust MRC Building, Cambridge CB2 2XY, United Kingdom
    Annu Rev Pharmacol Toxicol 47:629-56. 2007
    ..Here we review the background and work to date on this class of mitochondria-targeted antioxidants...
  49. doi request reprint A mitochondria-targeted mass spectrometry probe to detect glyoxals: implications for diabetes
    Pamela Boon Li Pun
    MRC Mitochondrial Biology Unit, Wellcome Trust MRC Building, Cambridge CB2 0XY, UK
    Free Radic Biol Med 67:437-50. 2014
    ....
  50. pmc DNA damage links mitochondrial dysfunction to atherosclerosis and the metabolic syndrome
    John R Mercer
    Division of Cardiovascular Medicine, University of Cambridge, Box 110, Addenbrooke s Hospital, Cambridge, CB2 2QQ, United Kingdom
    Circ Res 107:1021-31. 2010
    ..DNA damage is present in both genomic and mitochondrial DNA in atherosclerosis. However, whether DNA damage itself promotes atherosclerosis, or is simply a byproduct of the risk factors that promote atherosclerosis, is unknown...
  51. ncbi request reprint Delivery of antisense peptide nucleic acids (PNAs) to the cytosol by disulphide conjugation to a lipophilic cation
    Aleksandra Filipovska
    Medical Research Council Dunn Human Nutrition Unit, Wellcome Trust MRC Building, Hills Road, CB2 2XY, Cambridge, UK
    FEBS Lett 556:180-6. 2004
    ..This method of directing PNAs into cells is a significant improvement on current procedures and will facilitate in vitro and pharmacological applications of PNAs...
  52. pmc Does interplay between nitric oxide and mitochondria affect hypoxia-inducible transcription factor-1 activity?
    Michael P Murphy
    MRC Dunn Human Nutrition Unit, Wellcome Trust MRC Building, Hills Road, Cambridge CB2 2XY, UK
    Biochem J 376:e5-6. 2003
    ..These data resolve reported discrepancies on the effect of NO on HIF-1alpha stability at low oxygen concentrations and suggest that inhibition of mitochondrial respiration by NO may affect oxygen sensing by HIF-1 in vivo...
  53. ncbi request reprint Modulating mitochondrial intracellular location as a redox signal
    Michael P Murphy
    Medical Research Council, Mitochondrial Biology Unit, Wellcome Trust MRC Building, Hills Road, Cambridge CB2 0XY, UK
    Sci Signal 5:pe39. 2012
    ..This paper and other recent work suggest a new twist on mitochondrial signaling: that the redistribution of mitochondria within the cell can be a component of regulatory pathways...
  54. pmc A redox switch in angiotensinogen modulates angiotensin release
    Aiwu Zhou
    Department of Haematology, Cambridge Institute for Medical Research, University of Cambridge, Hills Road, Cambridge CB2 0XY, UK
    Nature 468:108-11. 2010
    ....
  55. pmc Sequence-specific modification of mitochondrial DNA using a chimeric zinc finger methylase
    Michal Minczuk
    Medical Research Council Laboratory of Molecular Biology, Hills Road, Cambridge CB2 2QH, United Kingdom
    Proc Natl Acad Sci U S A 103:19689-94. 2006
    ..This is a proof of principle that it is possible to target and alter mtDNA in a sequence-specific manner by using zinc finger technology...
  56. ncbi request reprint Interactions of mitochondrial thiols with nitric oxide
    Nikola J Costa
    Human Nutrition Unit, Wellcome Trust MRC Building, Cambridge, UK
    Antioxid Redox Signal 5:291-305. 2003
    ..Here we review mitochondrial thiol metabolism, outline how NO and its metabolites interact with thiols, and discuss the implications of these reactions for mitochondrial and cell function...
  57. ncbi request reprint Specific targeting of a DNA-alkylating reagent to mitochondria. Synthesis and characterization of [4-((11aS)-7-methoxy-1,2,3,11a-tetrahydro-5H-pyrrolo[2,1-c][1,4]benzodiazepin-5-on-8-oxy)butyl]-triphenylphosphonium iodide
    Andrew M James
    MRC Dunn Human Nutrition Unit, Wellcome Trust MRC Building, Cambridge, UK
    Eur J Biochem 270:2827-36. 2003
    ..This inability to alkylate mtDNA in situ has significant implications for the design of therapies for mtDNA diseases and for studies on the packaging, expression and turnover of mtDNA in general...
  58. pmc Delivery of bioactive molecules to mitochondria in vivo
    Robin A J Smith
    Medical Research Council Dunn Human Nutrition Unit, Wellcome Trust Medical Research Council Building, Hills Road, Cambridge CB2 2XY, United Kingdom
    Proc Natl Acad Sci U S A 100:5407-12. 2003
    ..This finding opens the way to the testing of mitochondria-specific therapies in mouse models of human degenerative diseases...
  59. ncbi request reprint Superoxide activates mitochondrial uncoupling protein 2 from the matrix side. Studies using targeted antioxidants
    Karim S Echtay
    Medical Research Council Dunn Human Nutrition Unit, Hills Road, Cambridge CB2 2XY, UK
    J Biol Chem 277:47129-35. 2002
    ..We conclude that superoxide (or its products) exerts its uncoupling effect by activating the proton transport mechanism of uncoupling proteins at the matrix side of the mitochondrial inner membrane...
  60. doi request reprint Combined therapeutic benefit of mitochondria-targeted antioxidant, MitoQ10, and angiotensin receptor blocker, losartan, on cardiovascular function
    Jennifer McLachlan
    aBHF Glasgow Cardiovascular Research Centre, University of Glasgow, Glasgow bMRC Mitochondrial Biology Unit, Cambridge, UK
    J Hypertens 32:555-64. 2014
    ..In parallel, we investigated the impact of MitoQ10 on cardiac hypertrophy in a neonatal cardiomyocyte cell line...
  61. doi request reprint Construction and testing of engineered zinc-finger proteins for sequence-specific modification of mtDNA
    Michal Minczuk
    Mitochondrial Biology Unit, MRC, Cambridge, UK
    Nat Protoc 5:342-56. 2010
    ..The protocol can be completed within a week, although time-consuming DNA cloning steps may extend this...
  62. doi request reprint Toward a control theory analysis of aging
    Michael P Murphy
    Medical Research Council Dunn Human Nutrition Unit, Cambridge, United Kingdom
    Annu Rev Biochem 77:777-98. 2008
    ..This combination of a hierarchical framework and the methodologies of MCA may prove useful both for thinking about aging and for analyzing it experimentally...
  63. ncbi request reprint How mitochondrial damage affects cell function
    Andrew M James
    MRC Dunn Human Nutrition Unit, Cambridge, UK
    J Biomed Sci 9:475-87. 2002
    ..A better understanding of these processes will eventually clarify why particular mtDNA mutations cause defined syndromes in some cases but not in others and why the same mutation can lead to different phenotypes...