M P Murphy

Summary

Affiliation: University of Cambridge
Country: UK

Publications

  1. pmc How mitochondria produce reactive oxygen species
    Michael P Murphy
    MRC Dunn Human Nutrition Unit, Hills Road, Cambridge CB20XY, UK
    Biochem J 417:1-13. 2009
  2. pmc Antioxidant properties of MitoTEMPOL and its hydroxylamine
    Jan Trnka
    MRC Dunn Human Nutrition Unit, Cambridge, UK
    Free Radic Res 43:4-12. 2009
  3. pmc Complex I within oxidatively stressed bovine heart mitochondria is glutathionylated on Cys-531 and Cys-704 of the 75-kDa subunit: potential role of CYS residues in decreasing oxidative damage
    Thomas R Hurd
    Medical Research Council Dunn Human Nutrition Unit, Wellcome Trust MRC Building, Hills Road, Cambridge CB2 0XY, United Kingdom
    J Biol Chem 283:24801-15. 2008
  4. ncbi request reprint Toward a control theory analysis of aging
    Michael P Murphy
    Medical Research Council Dunn Human Nutrition Unit, Cambridge, United Kingdom
    Annu Rev Biochem 77:777-98. 2008
  5. ncbi request reprint Targeting lipophilic cations to mitochondria
    Michael P Murphy
    MRC Dunn Human Nutrition Unit, Wellcome Trust MRC Building, Hills Road, Cambridge CB2 0XY, United Kingdom
    Biochim Biophys Acta 1777:1028-31. 2008
  6. pmc Does interplay between nitric oxide and mitochondria affect hypoxia-inducible transcription factor-1 activity?
    Michael P Murphy
    MRC Dunn Human Nutrition Unit, Wellcome Trust MRC Building, Hills Road, Cambridge CB2 2XY, UK
    Biochem J 376:e5-6. 2003
  7. ncbi request reprint Superoxide activates uncoupling proteins by generating carbon-centered radicals and initiating lipid peroxidation: studies using a mitochondria-targeted spin trap derived from alpha-phenyl-N-tert-butylnitrone
    Michael P Murphy
    Medical Research Council Dunn Human Nutrition Unit, Wellcome Trust MRC Building, Hills Road, Cambridge CB2 2XY, United Kingdom
    J Biol Chem 278:48534-45. 2003
  8. ncbi request reprint Investigating mitochondrial radical production using targeted probes
    M P Murphy
    Medical Research Council Dunn Human Nutrition Unit, Hills Road, Cambridge CB2 2XY, UK
    Biochem Soc Trans 32:1011-4. 2004
  9. ncbi request reprint Development of lipophilic cations as therapies for disorders due to mitochondrial dysfunction
    M P Murphy
    MRC Dunn Human Nutrition Unit, Wellcome Trust MRC Building, Hills Road, Cambridge CB2 2XY, UK
    Expert Opin Biol Ther 1:753-64. 2001
  10. ncbi request reprint Targeting antioxidants to mitochondria by conjugation to lipophilic cations
    Michael P Murphy
    MRC Dunn Human Nutrition Unit, Wellcome Trust MRC Building, Cambridge CB2 2XY, United Kingdom
    Annu Rev Pharmacol Toxicol 47:629-56. 2007

Collaborators

Detail Information

Publications83

  1. pmc How mitochondria produce reactive oxygen species
    Michael P Murphy
    MRC Dunn Human Nutrition Unit, Hills Road, Cambridge CB20XY, UK
    Biochem J 417:1-13. 2009
    ....
  2. pmc Antioxidant properties of MitoTEMPOL and its hydroxylamine
    Jan Trnka
    MRC Dunn Human Nutrition Unit, Cambridge, UK
    Free Radic Res 43:4-12. 2009
    ..Furthermore, as TEMPOL is also reduced to a hydroxylamine in vivo, many of its antioxidant effects may also be mediated by its hydroxylamine...
  3. pmc Complex I within oxidatively stressed bovine heart mitochondria is glutathionylated on Cys-531 and Cys-704 of the 75-kDa subunit: potential role of CYS residues in decreasing oxidative damage
    Thomas R Hurd
    Medical Research Council Dunn Human Nutrition Unit, Wellcome Trust MRC Building, Hills Road, Cambridge CB2 0XY, United Kingdom
    J Biol Chem 283:24801-15. 2008
    ....
  4. ncbi request reprint Toward a control theory analysis of aging
    Michael P Murphy
    Medical Research Council Dunn Human Nutrition Unit, Cambridge, United Kingdom
    Annu Rev Biochem 77:777-98. 2008
    ..This combination of a hierarchical framework and the methodologies of MCA may prove useful both for thinking about aging and for analyzing it experimentally...
  5. ncbi request reprint Targeting lipophilic cations to mitochondria
    Michael P Murphy
    MRC Dunn Human Nutrition Unit, Wellcome Trust MRC Building, Hills Road, Cambridge CB2 0XY, United Kingdom
    Biochim Biophys Acta 1777:1028-31. 2008
    ..Here we outline some of the background to the development of these compounds...
  6. pmc Does interplay between nitric oxide and mitochondria affect hypoxia-inducible transcription factor-1 activity?
    Michael P Murphy
    MRC Dunn Human Nutrition Unit, Wellcome Trust MRC Building, Hills Road, Cambridge CB2 2XY, UK
    Biochem J 376:e5-6. 2003
    ..These data resolve reported discrepancies on the effect of NO on HIF-1alpha stability at low oxygen concentrations and suggest that inhibition of mitochondrial respiration by NO may affect oxygen sensing by HIF-1 in vivo...
  7. ncbi request reprint Superoxide activates uncoupling proteins by generating carbon-centered radicals and initiating lipid peroxidation: studies using a mitochondria-targeted spin trap derived from alpha-phenyl-N-tert-butylnitrone
    Michael P Murphy
    Medical Research Council Dunn Human Nutrition Unit, Wellcome Trust MRC Building, Hills Road, Cambridge CB2 2XY, United Kingdom
    J Biol Chem 278:48534-45. 2003
    ..Superoxide releases iron from iron-sulfur center proteins, which then generates carbon-centered radicals that initiate lipid peroxidation, yielding breakdown products that activate UCPs...
  8. ncbi request reprint Investigating mitochondrial radical production using targeted probes
    M P Murphy
    Medical Research Council Dunn Human Nutrition Unit, Hills Road, Cambridge CB2 2XY, UK
    Biochem Soc Trans 32:1011-4. 2004
    ..The selective mitochondrial localization of these compounds enables us to investigate several aspects of the production of reactive oxygen species by mitochondria...
  9. ncbi request reprint Development of lipophilic cations as therapies for disorders due to mitochondrial dysfunction
    M P Murphy
    MRC Dunn Human Nutrition Unit, Wellcome Trust MRC Building, Hills Road, Cambridge CB2 2XY, UK
    Expert Opin Biol Ther 1:753-64. 2001
    ..Mitochondria-targeted lipophilic cations may lead to improved therapies for diseases involving mitochondrial dysfunction...
  10. ncbi request reprint Targeting antioxidants to mitochondria by conjugation to lipophilic cations
    Michael P Murphy
    MRC Dunn Human Nutrition Unit, Wellcome Trust MRC Building, Cambridge CB2 2XY, United Kingdom
    Annu Rev Pharmacol Toxicol 47:629-56. 2007
    ..Here we review the background and work to date on this class of mitochondria-targeted antioxidants...
  11. ncbi request reprint Interactions of mitochondria-targeted and untargeted ubiquinones with the mitochondrial respiratory chain and reactive oxygen species. Implications for the use of exogenous ubiquinones as therapies and experimental tools
    Andrew M James
    Medical Research Council Dunn Human Nutrition Unit, Wellcome Trust MRC Building, Hills Road, Cambridge CB2 2XY, United Kingdom
    J Biol Chem 280:21295-312. 2005
    ..Our results comprehensively describe the interaction of exogenous ubiquinones with mitochondria and have implications for their rational design and use as therapies and as research tools to probe mitochondrial function...
  12. ncbi request reprint Superoxide activates mitochondrial uncoupling protein 2 from the matrix side. Studies using targeted antioxidants
    Karim S Echtay
    Medical Research Council Dunn Human Nutrition Unit, Hills Road, Cambridge CB2 2XY, UK
    J Biol Chem 277:47129-35. 2002
    ..We conclude that superoxide (or its products) exerts its uncoupling effect by activating the proton transport mechanism of uncoupling proteins at the matrix side of the mitochondrial inner membrane...
  13. ncbi request reprint A mitochondria-targeted nitroxide is reduced to its hydroxylamine by ubiquinol in mitochondria
    Jan Trnka
    Medical Research Council Dunn Human Nutrition Unit, Wellcome Trust MRC Building, Hills Road, Cambridge CB2 0XY, UK
    Free Radic Biol Med 44:1406-19. 2008
    ....
  14. ncbi request reprint Lipophilic triphenylphosphonium cations as tools in mitochondrial bioenergetics and free radical biology
    M F Ross
    MRC Dunn Human Nutrition Unit, Cambridge, CB2 2XY, UK
    Biochemistry (Mosc) 70:222-30. 2005
    ..Here we review why these molecules are useful in mitochondrial research and outline some of the ways in which they are now being utilized...
  15. ncbi request reprint Mitochondria-targeted redox probes as tools in the study of oxidative damage and ageing
    Andrew M James
    Medical Research Council Dunn, Hills Road, Cambridge CB2 2XY, UK
    Mech Ageing Dev 126:982-6. 2005
    ..Here, we outline how the formation and consequences of mitochondrial ROS production can be investigated using these probes...
  16. pmc Accumulation of lipophilic dications by mitochondria and cells
    Meredith F Ross
    Medical Research Council Dunn Human Nutrition Unit, Wellcome Trust MRC Building, Hills Road, Cambridge CB2 2XY, UK
    Biochem J 400:199-208. 2006
    ..Instead, this suggests that it may be possible to form dications within mitochondria that then remain within the cell...
  17. ncbi request reprint Mitochondrial targeting of quinones: therapeutic implications
    Helena M Cocheme
    MRC Dunn Human Nutrition Unit, Wellcome Trust MRC Building, Hills Road, Cambridge CB2 2XY, UK
    Mitochondrion 7:S94-102. 2007
    ..MitoQ protects mitochondria against oxidative damage in vitro and following oral delivery, and may therefore form the basis for mitochondria-protective therapies...
  18. ncbi request reprint Specific targeting of a DNA-alkylating reagent to mitochondria. Synthesis and characterization of [4-((11aS)-7-methoxy-1,2,3,11a-tetrahydro-5H-pyrrolo[2,1-c][1,4]benzodiazepin-5-on-8-oxy)butyl]-triphenylphosphonium iodide
    Andrew M James
    MRC Dunn Human Nutrition Unit, Wellcome Trust MRC Building, Cambridge, UK
    Eur J Biochem 270:2827-36. 2003
    ..This inability to alkylate mtDNA in situ has significant implications for the design of therapies for mtDNA diseases and for studies on the packaging, expression and turnover of mtDNA in general...
  19. ncbi request reprint Disulphide formation on mitochondrial protein thiols
    T R Hurd
    Medical Research Council Dunn Human Nutrition Unit, Hills Road, Cambridge CB2 2XY, UK
    Biochem Soc Trans 33:1390-3. 2005
    ..In the present study, we review the mechanisms and physiological significance of these processes...
  20. pmc Development of a single-chain, quasi-dimeric zinc-finger nuclease for the selective degradation of mutated human mitochondrial DNA
    Michal Minczuk
    MRC Laboratory of Molecular Biology, Hills Road, Cambridge CB2 2QH, UK
    Nucleic Acids Res 36:3926-38. 2008
    ..Therefore, mitochondria-targeted single-chain ZFNs are a promising candidate approach for the treatment of mtDNA diseases...
  21. pmc A redox switch in angiotensinogen modulates angiotensin release
    Aiwu Zhou
    Department of Haematology, Cambridge Institute for Medical Research, University of Cambridge, Hills Road, Cambridge CB2 0XY, UK
    Nature 468:108-11. 2010
    ....
  22. pmc Sequence-specific modification of mitochondrial DNA using a chimeric zinc finger methylase
    Michal Minczuk
    Medical Research Council Laboratory of Molecular Biology, Hills Road, Cambridge CB2 2QH, United Kingdom
    Proc Natl Acad Sci U S A 103:19689-94. 2006
    ..This is a proof of principle that it is possible to target and alter mtDNA in a sequence-specific manner by using zinc finger technology...
  23. ncbi request reprint Interaction of the mitochondria-targeted antioxidant MitoQ with phospholipid bilayers and ubiquinone oxidoreductases
    Andrew M James
    Medical Research Council Dunn Human Nutrition Unit, Wellcome Trust MRC Building, Hills Road, Cambridge CB2 2XY, United Kingdom
    J Biol Chem 282:14708-18. 2007
    ..These findings will facilitate the rational design of future mitochondria-targeted molecules...
  24. ncbi request reprint Targeting lipoic acid to mitochondria: synthesis and characterization of a triphenylphosphonium-conjugated alpha-lipoyl derivative
    Stephanie E Brown
    Medical Research Council Dunn Human Nutrition Unit, Wellcome Trust MRC Building, Hills Road, Cambridge CB2 2XY, UK
    Free Radic Biol Med 42:1766-80. 2007
    ..These results suggest that the protective action of LA in vivo may require its reduction to LAH(2) and that this reduction is largely mediated by TrxR...
  25. ncbi request reprint Complementation of coenzyme Q-deficient yeast by coenzyme Q analogues requires the isoprenoid side chain
    Andrew M James
    Wellcome Trust MRC Building, Cambridge, UK
    FEBS J 277:2067-82. 2010
    ..Using a DeltaCoQ double-knockout library we identified a number of genes that decrease the ability of yeast to grow on exogenous CoQ. Here we suggest that CoQ or its redox state may be a signal for growth during the shift to respiration...
  26. ncbi request reprint Glutaredoxin 2 catalyzes the reversible oxidation and glutathionylation of mitochondrial membrane thiol proteins: implications for mitochondrial redox regulation and antioxidant DEFENSE
    Samantha M Beer
    Medical Research Council Dunn Human Nutrition Unit, Wellcome Trust MRC Building, Hills Road, Cambridge CB2 2XY, United Kingdom
    J Biol Chem 279:47939-51. 2004
    ..Our findings indicate that Grx2 plays a central role in the response of mitochondria to both redox signals and oxidative stress by facilitating the interplay between the mitochondrial glutathione pool and protein thiols...
  27. ncbi request reprint Fine-tuning the hydrophobicity of a mitochondria-targeted antioxidant
    Jordi Asin-Cayuela
    Medical Research Council Dunn Human Nutrition Unit, Wellcome Trust MRC Building, Hills Road, Cambridge CB2 2XY, UK
    FEBS Lett 571:9-16. 2004
    ..This indicates how the uptake and distribution of mitochondria-targeted compounds within mitochondria and cells can be controlled, thereby facilitating investigations of mitochondrial oxidative damage...
  28. ncbi request reprint Rapid and extensive uptake and activation of hydrophobic triphenylphosphonium cations within cells
    Meredith F Ross
    Medical Research Council Dunn Human Nutrition Unit, Wellcome Trust MRC Building, Hills Road, Cambridge CB2 0XY, UK
    Biochem J 411:633-45. 2008
    ..The strong influence of hydrophobicity on TPP cation uptake into mitochondria within cells facilitates the rational design of mitochondria-targeted compounds to report on and modify mitochondrial function in vivo...
  29. ncbi request reprint Construction and testing of engineered zinc-finger proteins for sequence-specific modification of mtDNA
    Michal Minczuk
    Mitochondrial Biology Unit, MRC, Cambridge, UK
    Nat Protoc 5:342-56. 2010
    ..The protocol can be completed within a week, although time-consuming DNA cloning steps may extend this...
  30. ncbi request reprint Antioxidant and prooxidant properties of mitochondrial Coenzyme Q
    Andrew M James
    Medical Research Council Dunn Human Nutrition Unit, Cambridge, UK
    Arch Biochem Biophys 423:47-56. 2004
    ..To help understand how Coenzyme Q contributes to both mitochondrial oxidative damage and antioxidant defences, we have reviewed its antioxidant and prooxidant properties...
  31. pmc Antioxidants can inhibit basal autophagy and enhance neurodegeneration in models of polyglutamine disease
    Benjamin R Underwood
    Department of Medical Genetics, University of Cambridge, Cambridge Institute for Medical Research, Addenbrooke s Hospital, Hills Road, Cambridge CB2 0XY, UK
    Hum Mol Genet 19:3413-29. 2010
    ..Thus, the potential benefits in neurodegenerative diseases of some classes of antioxidants may be compromised by their autophagy-blocking properties...
  32. ncbi request reprint Complex I is the major site of mitochondrial superoxide production by paraquat
    Helena M Cocheme
    Medical Research Council Dunn Human Nutrition Unit, Wellcome Trust MRC Building, Hills Road, Cambridge CB2 0XY, United Kingdom
    J Biol Chem 283:1786-98. 2008
    ..Together this membrane potential-dependent uptake across the mitochondrial inner membrane and the subsequent rapid reduction to the paraquat radical cation explain the toxicity of paraquat to mitochondria...
  33. pmc Identification of S-nitrosated mitochondrial proteins by S-nitrosothiol difference in gel electrophoresis (SNO-DIGE): implications for the regulation of mitochondrial function by reversible S-nitrosation
    Edward T Chouchani
    MRC Mitochondrial Biology Unit, Cambridge, UK
    Biochem J 430:49-59. 2010
    ..More generally, the development of SNO-DIGE facilitates robust investigation of protein S-nitrosation across the proteome...
  34. ncbi request reprint Persistent S-nitrosation of complex I and other mitochondrial membrane proteins by S-nitrosothiols but not nitric oxide or peroxynitrite: implications for the interaction of nitric oxide with mitochondria
    Christina C Dahm
    Medical Research Council Dunn Human Nutrition Unit, Wellcome Trust Medical Research Council Building, Hills Road, Cambridge CB2 2XY, United Kingdom
    J Biol Chem 281:10056-65. 2006
    ..These findings point to a significant role for complex I S-nitrosation and consequent dysfunction during nitrosative stress in disorders such as Parkinson disease and sepsis...
  35. pmc Cell-penetrating peptides do not cross mitochondrial membranes even when conjugated to a lipophilic cation: evidence against direct passage through phospholipid bilayers
    Meredith F Ross
    MRC Dunn Human Nutrition Unit, Wellcome Trust MRC Building, Hills Road, Cambridge CB2 2XY, UK
    Biochem J 383:457-68. 2004
    ....
  36. ncbi request reprint Prevention of mitochondrial oxidative damage as a therapeutic strategy in diabetes
    Katherine Green
    Medical Research Council Dunn Human Nutrition Unit, Cambridge, UK
    Diabetes 53:S110-8. 2004
    ..Here, we outline the background to these strategies and discuss how antioxidants targeted to mitochondria, or selective mitochondrial uncoupling, may be potential therapies for diabetes...
  37. pmc The essential function of the small Tim proteins in the TIM22 import pathway does not depend on formation of the soluble 70-kilodalton complex
    M P Murphy
    MRC Dunn Human Nutrition Unit, MRC Wellcome, Cambridge CB2 2XY, United Kingdom
    Mol Cell Biol 21:6132-8. 2001
    ....
  38. ncbi request reprint Detection of reactive oxygen species-sensitive thiol proteins by redox difference gel electrophoresis: implications for mitochondrial redox signaling
    Thomas R Hurd
    Medical Research Council Dunn Human Nutrition Unit, Wellcome Trust Medical Research Council Building, Hills Road, Cambridge, UK
    J Biol Chem 282:22040-51. 2007
    ..Thiol redox modification affected enzyme activity, suggesting that the reversible modification of enzyme activity by ROS from the respiratory chain may be an important and unexplored mode of mitochondrial redox signaling...
  39. ncbi request reprint Measuring mitochondrial protein thiol redox state
    Raquel Requejo
    Medical Research Council Mitochondrial Biology Unit, Wellcome Trust MRC Building, Cambridge, UK
    Methods Enzymol 474:123-47. 2010
    ..Here we outline some of the approaches that can be used to accomplish these goals and thereby infer the multiple roles of mammalian mitochondrial protein thiols in antioxidant defense and redox signaling...
  40. pmc A mitochondria-targeted S-nitrosothiol modulates respiration, nitrosates thiols, and protects against ischemia-reperfusion injury
    Tracy A Prime
    MRC Mitochondrial Biology Unit, Hills Road, Cambridge CB2 0XY, United Kingdom
    Proc Natl Acad Sci U S A 106:10764-9. 2009
    ..These results support the idea that selectively targeting NO(*) donors to mitochondria is an effective strategy to reversibly modulate respiration and to protect mitochondria against ischemia-reperfusion injury...
  41. ncbi request reprint Reversible glutathionylation of complex I increases mitochondrial superoxide formation
    Ellen R Taylor
    Medical Research Council Dunn Human Nutrition Unit, Wellcome Trust MRC Bldg, Hills Rd, Cambridge CB2 2XY, United Kingdom
    J Biol Chem 278:19603-10. 2003
    ....
  42. ncbi request reprint Glutathionylation of mitochondrial proteins
    Thomas R Hurd
    MRC Dunn Human Nutrition Unit, Cambridge, UK
    Antioxid Redox Signal 7:999-1010. 2005
    ..Here we review the mechanisms and physiological significance of the glutathionylation of mitochondrial thiol proteins...
  43. pmc Delivery of bioactive molecules to mitochondria in vivo
    Robin A J Smith
    Medical Research Council Dunn Human Nutrition Unit, Wellcome Trust Medical Research Council Building, Hills Road, Cambridge CB2 2XY, United Kingdom
    Proc Natl Acad Sci U S A 100:5407-12. 2003
    ..This finding opens the way to the testing of mitochondria-specific therapies in mouse models of human degenerative diseases...
  44. ncbi request reprint Interactions of mitochondrial thiols with nitric oxide
    Nikola J Costa
    Human Nutrition Unit, Wellcome Trust MRC Building, Cambridge, UK
    Antioxid Redox Signal 5:291-305. 2003
    ..Here we review mitochondrial thiol metabolism, outline how NO and its metabolites interact with thiols, and discuss the implications of these reactions for mitochondrial and cell function...
  45. ncbi request reprint How mitochondrial damage affects cell function
    Andrew M James
    MRC Dunn Human Nutrition Unit, Cambridge, UK
    J Biomed Sci 9:475-87. 2002
    ..A better understanding of these processes will eventually clarify why particular mtDNA mutations cause defined syndromes in some cases but not in others and why the same mutation can lead to different phenotypes...
  46. ncbi request reprint Radiosynthesis of 11-[(18) F]fluoroundecyltriphenylphosphonium (MitoF) as a potential mitochondria-specific positron emission tomography radiotracer
    L Li
    Molecular Imaging Chemistry Laboratory, Wolfson Brain Imaging Centre, University of Cambridge, Cambridge, UK
    J Labelled Comp Radiopharm 56:717-21. 2013
    ....
  47. ncbi request reprint Cell-penetrating peptides are excluded from the mitochondrial matrix
    M F Ross
    MRC Dunn Human Nutrition Unit, Wellcome Trust MRC Building, Hills Road, Cambridge CB2 2XY, UK
    Biochem Soc Trans 32:1072-4. 2004
    ..This result suggests that CPPs are unsuitable as mitochondria-targeting vectors, and implies an endocytic mode of cellular uptake for CPPs...
  48. ncbi request reprint Synthesis and characterization of a triphenylphosphonium-conjugated peroxidase mimetic. Insights into the interaction of ebselen with mitochondria
    Aleksandra Filipovska
    Medical Research Council Dunn Human Nutrition Unit, Wellcome Trust MRC Bldg, Hills Rd, Cambridge CB2 2XY, United Kingdom
    J Biol Chem 280:24113-26. 2005
    ..This exploration has led to new insights into the behavior of peroxidase mimetics within mitochondria and to their use in investigating mitochondrial oxidative damage...
  49. ncbi request reprint Chapter 19 Measuring redox changes to mitochondrial protein thiols with redox difference gel electrophoresis (redox-DIGE)
    Thomas R Hurd
    The Medical Research Council Mitochondrial Biology Unit, Cambridge, United Kingdom
    Methods Enzymol 456:343-61. 2009
    ..Importantly, this method is not only applicable to studies in isolated mitochondria but can also be applied to more complicated systems such as intact cells and perhaps even whole organisms...
  50. pmc Cysteine residues exposed on protein surfaces are the dominant intramitochondrial thiol and may protect against oxidative damage
    Raquel Requejo
    MRC Mitochondrial Biology Unit, Wellcome Trust MRC Building, Cambridge, UK
    FEBS J 277:1465-80. 2010
    ..Therefore, exposed protein thiols are the dominant free thiol within the cell and may play a critical role in intracellular antioxidant defences against oxidative damage...
  51. ncbi request reprint Delivery of antisense peptide nucleic acids (PNAs) to the cytosol by disulphide conjugation to a lipophilic cation
    Aleksandra Filipovska
    Medical Research Council Dunn Human Nutrition Unit, Wellcome Trust MRC Building, Hills Road, CB2 2XY, Cambridge, UK
    FEBS Lett 556:180-6. 2004
    ..This method of directing PNAs into cells is a significant improvement on current procedures and will facilitate in vitro and pharmacological applications of PNAs...
  52. doi request reprint Quantification and identification of mitochondrial proteins containing vicinal dithiols
    Raquel Requejo
    MRC Mitochondrial Biology Unit, Cambridge, UK
    Arch Biochem Biophys 504:228-35. 2010
    ..These findings are consistent with roles for mitochondrial vicinal dithiol proteins in antioxidant defence and redox signalling and these methodologies will enable these roles to be explored...
  53. pmc DNA damage links mitochondrial dysfunction to atherosclerosis and the metabolic syndrome
    John R Mercer
    Division of Cardiovascular Medicine, University of Cambridge, Box 110, Addenbrooke s Hospital, Cambridge, CB2 2QQ, United Kingdom
    Circ Res 107:1021-31. 2010
    ..DNA damage is present in both genomic and mitochondrial DNA in atherosclerosis. However, whether DNA damage itself promotes atherosclerosis, or is simply a byproduct of the risk factors that promote atherosclerosis, is unknown...
  54. pmc Mitochondrial uncouplers with an extraordinary dynamic range
    Phing How Lou
    MRC Dunn Human Nutrition Unit, Hills Road, Cambridge CB2 2XY, UK
    Biochem J 407:129-40. 2007
    ....
  55. ncbi request reprint Prevention of mitochondrial oxidative damage using targeted antioxidants
    Geoffrey F Kelso
    Department of Chemistry, University of Otago, Box 56, Dunedin, New Zealand
    Ann N Y Acad Sci 959:263-74. 2002
    ..When fed to mice these compounds accumulated within the brain, heart, and liver; therefore, using these mitochondrial-targeted antioxidants may help investigations of the role of mitochondrial oxidative damage in animal models of aging...
  56. ncbi request reprint Targeting an antioxidant to mitochondria decreases cardiac ischemia-reperfusion injury
    Victoria J Adlam
    Department of Chemistry, University of Otago, Dunedin, New Zealand
    FASEB J 19:1088-95. 2005
    ....
  57. ncbi request reprint Induction of mitochondrial ROS production by electrophilic lipids: a new pathway of redox signaling?
    Michael P Murphy
    Am J Physiol Heart Circ Physiol 290:H1754-5. 2006
  58. ncbi request reprint The effects of exogenous antioxidants on lifespan and oxidative stress resistance in Drosophila melanogaster
    Tapiwanashe Magwere
    Centre for Research on Ageing, University College London, Department of Biology, Darwin Building, Gower Street, London WC1E 6BT, United Kingdom
    Mech Ageing Dev 127:356-70. 2006
    ..These findings place significant constraints on the role of oxidative stress in normal ageing...
  59. ncbi request reprint MitoQ counteracts telomere shortening and elongates lifespan of fibroblasts under mild oxidative stress
    Gabriele Saretzki
    Institute of Aging and Health, Newcastle University, Newcastle upon Tyne NE4 6BE, UK
    Aging Cell 2:141-3. 2003
  60. ncbi request reprint Mitochondria-targeted antioxidants protect Friedreich Ataxia fibroblasts from endogenous oxidative stress more effectively than untargeted antioxidants
    Matthias L Jauslin
    MyoContract Ltd, CH 4410 Liestal, Switzerland
    FASEB J 17:1972-4. 2003
    ..Targeted antioxidants may have therapeutic potential in FRDA and in other disorders involving mitochondrial oxidative damage...
  61. ncbi request reprint Targeting dinitrophenol to mitochondria: limitations to the development of a self-limiting mitochondrial protonophore
    Frances H Blaikie
    Department of Chemistry, University of Otago, P O Box 56, Dunedin, New Zealand
    Biosci Rep 26:231-43. 2006
    ..Therefore MitoDNP did not act as a protonophore, probably because the efflux of deprotonated MitoDNP was inhibited...
  62. ncbi request reprint Specific modification of mitochondrial protein thiols in response to oxidative stress: a proteomics approach
    Tsu Kung Lin
    Department of Biochemistry, University of Otago, Box 56, Dunedin, New Zealand
    J Biol Chem 277:17048-56. 2002
    ..In addition the methodology has the potential to identify novel redox-dependent modulation of mitochondrial proteins...
  63. ncbi request reprint Antioxidant and pro-oxidant properties of pyrroloquinoline quinone (PQQ): implications for its function in biological systems
    Kai He
    Department of Medical and Surgical Sciences, Dunedin School of Medicine, University of Otago, P O Box 56, Dunedin, New Zealand
    Biochem Pharmacol 65:67-74. 2003
    ..We conclude that the reactivities of PQQ are dependent on its environment and that it can act as an antioxidant or a pro-oxidant in different biological systems...
  64. ncbi request reprint Targeting coenzyme Q derivatives to mitochondria
    Robin A J Smith
    Department of Chemistry, University of Otago, Dunedin, New Zealand
    Methods Enzymol 382:45-67. 2004
  65. ncbi request reprint Mitochondrial function is required for hydrogen peroxide-induced growth factor receptor transactivation and downstream signaling
    Kai Chen
    Evans Memorial Department of Medicine and Whitaker Cardiovascular Institute, Boston University School of Medicine, Boston, 715 Albany Street, MA 02118, USA
    J Biol Chem 279:35079-86. 2004
    ..These data establish a novel role for the mitochondrion as a proximal target specific to H(2)O(2)-induced signaling and growth factor transactivation...
  66. ncbi request reprint Mitochondrial redox state regulates transcription of the nuclear-encoded mitochondrial protein manganese superoxide dismutase: a proposed adaptive response to mitochondrial redox imbalance
    Aekyong Kim
    Molecular and Environmental Toxicology Center, University of Wisconsin, Madison, WI, USA
    Free Radic Biol Med 38:644-54. 2005
    ..Therefore, the mitochondrial redox state appears to modulate a nuclear-driven biochemical event, i.e., transcriptional activation of a nuclear gene encoding a protein targeted to mitochondria...
  67. ncbi request reprint A targeted antioxidant reveals the importance of mitochondrial reactive oxygen species in the hypoxic signaling of HIF-1alpha
    Alejandra Sanjuan-Pla
    Unidad Mixta de Investigación Centro Nacional de Investigaciones Cardiovasculares Carlos III Universitat de Valencia, Spain
    FEBS Lett 579:2669-74. 2005
    ..This study strongly suggests that mitochondrial ROS contribute to the hypoxic stabilization of HIF-1alpha...
  68. ncbi request reprint Mitochondria-derived reactive oxygen species mediate blue light-induced death of retinal pigment epithelial cells
    Ayala King
    F M Kirby Center for Molecular Ophthalmology, Scheie Eye Institute, University of Pennsylvania, Philadelphia, PA 19104, USA
    Photochem Photobiol 79:470-5. 2004
    ..These results show that mitochondria are an important source of toxic oxygen radicals in blue light-exposed RPE cells and may indicate new approaches for treating AMD using mitochondria-targeted antioxidants...
  69. pmc The Qo site of the mitochondrial complex III is required for the transduction of hypoxic signaling via reactive oxygen species production
    Eric L Bell
    Department of Medicine, Northwestern University Medical School, Chicago, IL 60611, USA
    J Cell Biol 177:1029-36. 2007
    ..These results provide genetic and pharmacologic evidence that the Qo site of complex III is required for the transduction of hypoxic signal by releasing ROS to stabilize the HIF-1alpha protein...
  70. pmc Mitochondrial reactive oxygen species regulate the temporal activation of nuclear factor kappaB to modulate tumour necrosis factor-induced apoptosis: evidence from mitochondria-targeted antioxidants
    Gillian Hughes
    Department of Biochemistry, University of Otago, P O Box 56, Dunedin, New Zealand
    Biochem J 389:83-9. 2005
    ....
  71. ncbi request reprint Mitochondrial production of oxidants is necessary for physiological calcium oscillations
    M Cristina Camello-Almaraz
    Department of Physiology, Fac Veterinary Sciences, University of Extremadura, Caceres, Spain
    J Cell Physiol 206:487-94. 2006
    ..This study unveils a new and unexplored aspect of the participation of mitochondria in calcium signals...
  72. pmc Beta-amyloid mediated nitration of manganese superoxide dismutase: implication for oxidative stress in a APPNLH/NLH X PS-1P264L/P264L double knock-in mouse model of Alzheimer's disease
    Muthuswamy Anantharaman
    Graduate Center for Toxicology, University of Kentucky, Lexington 40536 0305, USA
    Am J Pathol 168:1608-18. 2006
    ..The compromised activity of MnSOD, a primary antioxidant enzyme protecting mitochondria, may explain mitochondrial dysfunction and provide the missing link between Abeta-induced oxidative stress and Alzheimer's disease...
  73. ncbi request reprint Effect of the mitochondrial antioxidant, Mito Vitamin E, on hypoxic-ischemic striatal injury in neonatal rats: a dose-response and stereological study
    Matthew V Covey
    Department of Anatomy and Structural Biology, University of Otago, PO Box 913, Dunedin, New Zealand
    Exp Neurol 199:513-9. 2006
    ..The results also suggest that mitochondrial oxidative damage does not contribute significantly to the death of striatal medium-spiny neurons after perinatal hypoxia-ischemia...
  74. ncbi request reprint Characterization of the mitochondrial respiratory pathways in Candida albicans
    Eva J Helmerhorst
    Goldman School of Dental Medicine, Boston University, 100 East Newton Street, Boston, MA 02118, USA
    Biochim Biophys Acta 1556:73-80. 2002
    ....
  75. ncbi request reprint Oxidative modification of hepatic mitochondria protein thiols: effect of chronic alcohol consumption
    Aparna Venkatraman
    Department of Pathology, University of Alabama at Birmingham, Birmingham, AL 35294, USA
    Am J Physiol Gastrointest Liver Physiol 286:G521-7. 2004
    ....
  76. ncbi request reprint Challenges for providing effective hemostasis in surgery and trauma
    Jeffrey H Lawson
    Department of Surgery, Duke University Medical Center, Durham, NC, USA
    Semin Hematol 41:55-64. 2004
    ..However, many of these agents have not yet been tested in clinical trials and studies are urgently needed to determine efficacy, safety, optimal dosage and time of administration...
  77. ncbi request reprint Mitochondria-targeted antioxidants do not prevent tumour necrosis factor-induced necrosis of L929 cells
    Reagan M Jarvis
    Department of Biochemistry, University of Otago, Dunedin, New Zealand
    Free Radic Res 41:1041-6. 2007
    ..Instead, these results suggest alternative mitochondrial functions, such as a respiration-dependent process, are critical for necrotic death...
  78. ncbi request reprint Inhibition of complex I of the electron transport chain causes O2-. -mediated mitochondrial outgrowth
    Werner J H Koopman
    Microscopal Imaging Center, Department of Biochemistry, Nijmegen Center for Molecular Life Sciences, Radboud University Medical Center, PO Box 9101, NL 6500 HB Nijmegen, The Netherlands
    Am J Physiol Cell Physiol 288:C1440-50. 2005
    ....
  79. ncbi request reprint The role of the renin-angiotensin system and oxidative stress in spontaneously hypertensive rat mesenteric collateral growth impairment
    Steven J Miller
    Department of Surgery, Indiana University Medical Center, 1001 West Tenth Street, Indianapolis, IN 46202 2879, USA
    Am J Physiol Heart Circ Physiol 292:H2523-31. 2007
    ....
  80. pmc Selective fluorescent imaging of superoxide in vivo using ethidium-based probes
    Kristine M Robinson
    Linus Pauling Institute, Department of Biochemistry and Biophysics, Oregon State University, Corvallis, OR 97331, USA
    Proc Natl Acad Sci U S A 103:15038-43. 2006
    ..Monitoring oxidation at both 396 and 510 nm of excitation wavelengths can facilitate the more selective detection of superoxide in vivo...
  81. ncbi request reprint A new hypertrophic mechanism of serotonin in cardiac myocytes: receptor-independent ROS generation
    Pascale Bianchi
    Cardiovascular Medicine Section, Boston University Medical Center, Boston, Massachusetts, USA
    FASEB J 19:641-3. 2005
    ..This newly recognized, receptor-independent mechanism of serotonin may contribute to myocardial remodeling and failure...
  82. ncbi request reprint The concomitant expression and availability of conventional and alternative, cyanide-insensitive, respiratory pathways in Candida albicans
    Eva J Helmerhorst
    Department of Periodontology and Oral Biology, Goldman School of Dental Medicine, Boston University, 700 Albany Street W201, Boston, MA 02118, USA
    Mitochondrion 5:200-11. 2005
    ..The availability of two electron pathways in C. albicans and the potential use of either pathway endows this pleomorphic fungus with another level at which it can rapidly adjust to altered environmental conditions...
  83. pmc Activation of mitogen-activated protein kinases by lysophosphatidylcholine-induced mitochondrial reactive oxygen species generation in endothelial cells
    Nobuo Watanabe
    Research Center for Advanced Science and Technology, University of Tokyo, 4 6 1 Komaba, Meguro, Tokyo 153 8904
    Am J Pathol 168:1737-48. 2006
    ....