G Murphy

Summary

Affiliation: University of East Anglia
Country: UK

Publications

  1. ncbi request reprint Evidence for a critical role of the tumor necrosis factor alpha convertase (TACE) in ectodomain shedding of the p75 neurotrophin receptor (p75NTR)
    Gisela Weskamp
    Cell Biology Program, Sloan Kettering Institute, Memorial Sloan Kettering Cancer Center, New York, New York 10021, USA
    J Biol Chem 279:4241-9. 2004
  2. ncbi request reprint Proteolysis and cell migration: creating a path?
    G Murphy
    School of Biological Sciences University of East Anglia Norwich, NR4 7TJ, UK
    Curr Opin Cell Biol 11:614-21. 1999
  3. pmc Matrix metalloproteinases in arthritic disease
    Gillian Murphy
    School of Biological Sciences, University of East Anglia, Norwich, UK
    Arthritis Res 4:S39-49. 2002
  4. ncbi request reprint Evaluation of some newer matrix metalloproteinases
    G Murphy
    School of Biological Sciences, University of East Anglia, Norwich, UK
    Ann N Y Acad Sci 878:25-39. 1999
  5. ncbi request reprint Matrix metalloproteinases 19 and 20 cleave aggrecan and cartilage oligomeric matrix protein (COMP)
    J O Stracke
    School of Biological Sciences, University of East Anglia, Norwich NR4 7TJ, UK
    FEBS Lett 478:52-6. 2000
  6. pmc Activation of pro-(matrix metalloproteinase-2) (pro-MMP-2) by thrombin is membrane-type-MMP-dependent in human umbilical vein endothelial cells and generates a distinct 63 kDa active species
    M A Lafleur
    School of Biological Sciences, University of East Anglia, Norwich NR4 7TJ, UK
    Biochem J 357:107-15. 2001
  7. ncbi request reprint Catalytic activities of membrane-type 6 matrix metalloproteinase (MMP25)
    W R English
    School of Biological Sciences, University of East Anglia, Norwich NR4 7TJ, UK
    FEBS Lett 491:137-42. 2001
  8. ncbi request reprint Biochemical characterization of the catalytic domain of human matrix metalloproteinase 19. Evidence for a role as a potent basement membrane degrading enzyme
    J O Stracke
    School of Biological Sciences, University of East Anglia, Norwich, NR4 7TJ, United Kingdom
    J Biol Chem 275:14809-16. 2000
  9. ncbi request reprint The role of exon 5 in fibroblast collagenase (MMP-1) substrate specificity and inhibitor selectivity
    V Knauper
    University of East Anglia, School of Biological Sciences, Norwich, UK
    Eur J Biochem 268:1888-96. 2001
  10. ncbi request reprint Characterization of the role of the "MT-loop": an eight-amino acid insertion specific to progelatinase A (MMP2) activating membrane-type matrix metalloproteinases
    W R English
    School of Biological Sciences, University of East Anglia, University Plain, Norwich, Norfolk NR4 7TJ, United Kingdom
    J Biol Chem 276:42018-26. 2001

Collaborators

Detail Information

Publications80

  1. ncbi request reprint Evidence for a critical role of the tumor necrosis factor alpha convertase (TACE) in ectodomain shedding of the p75 neurotrophin receptor (p75NTR)
    Gisela Weskamp
    Cell Biology Program, Sloan Kettering Institute, Memorial Sloan Kettering Cancer Center, New York, New York 10021, USA
    J Biol Chem 279:4241-9. 2004
    ..Because ectodomain shedding of p75NTR releases a soluble ectodomain and could also be a prerequisite for its regulated intramembrane proteolysis, these findings may have important implications for the functional regulation of p75NTR...
  2. ncbi request reprint Proteolysis and cell migration: creating a path?
    G Murphy
    School of Biological Sciences University of East Anglia Norwich, NR4 7TJ, UK
    Curr Opin Cell Biol 11:614-21. 1999
    ....
  3. pmc Matrix metalloproteinases in arthritic disease
    Gillian Murphy
    School of Biological Sciences, University of East Anglia, Norwich, UK
    Arthritis Res 4:S39-49. 2002
    ....
  4. ncbi request reprint Evaluation of some newer matrix metalloproteinases
    G Murphy
    School of Biological Sciences, University of East Anglia, Norwich, UK
    Ann N Y Acad Sci 878:25-39. 1999
    ..MMP19 shows a limited sequence identity with other MMPs and may represent a novel subclass. However, analysis of the recombinant protein identified a number of biochemical properties typical of the MMP family...
  5. ncbi request reprint Matrix metalloproteinases 19 and 20 cleave aggrecan and cartilage oligomeric matrix protein (COMP)
    J O Stracke
    School of Biological Sciences, University of East Anglia, Norwich NR4 7TJ, UK
    FEBS Lett 478:52-6. 2000
    ....
  6. pmc Activation of pro-(matrix metalloproteinase-2) (pro-MMP-2) by thrombin is membrane-type-MMP-dependent in human umbilical vein endothelial cells and generates a distinct 63 kDa active species
    M A Lafleur
    School of Biological Sciences, University of East Anglia, Norwich NR4 7TJ, UK
    Biochem J 357:107-15. 2001
    ..The integration of these proteinase systems in the vascular endothelium may be important during thrombogenesis and tissue remodelling associated with neovascularization...
  7. ncbi request reprint Catalytic activities of membrane-type 6 matrix metalloproteinase (MMP25)
    W R English
    School of Biological Sciences, University of East Anglia, Norwich NR4 7TJ, UK
    FEBS Lett 491:137-42. 2001
    ..Our findings suggest that MT6-MMP could play a role in cellular migration and invasion of the extracellular matrix and basement membranes and its activity may be tightly regulated by all members of the TIMP family...
  8. ncbi request reprint Biochemical characterization of the catalytic domain of human matrix metalloproteinase 19. Evidence for a role as a potent basement membrane degrading enzyme
    J O Stracke
    School of Biological Sciences, University of East Anglia, Norwich, NR4 7TJ, United Kingdom
    J Biol Chem 275:14809-16. 2000
    ..Finally, and in contrast to studies with other MMPs, MMP-19 catalytic domain was not able to activate any of the latent MMPs tested in vitro...
  9. ncbi request reprint The role of exon 5 in fibroblast collagenase (MMP-1) substrate specificity and inhibitor selectivity
    V Knauper
    University of East Anglia, School of Biological Sciences, Norwich, UK
    Eur J Biochem 268:1888-96. 2001
    ..These data show that the region of MMP-1 coded for by exon 5 is involved in both substrate specificity and inhibitor selectivity and the structural basis for our findings is discussed...
  10. ncbi request reprint Characterization of the role of the "MT-loop": an eight-amino acid insertion specific to progelatinase A (MMP2) activating membrane-type matrix metalloproteinases
    W R English
    School of Biological Sciences, University of East Anglia, University Plain, Norwich, Norfolk NR4 7TJ, United Kingdom
    J Biol Chem 276:42018-26. 2001
    ..All the mutants examined were able process proGLA but, as found with the soluble forms, were kinetically impaired when compared with wild-type MT1-MMP...
  11. ncbi request reprint Kinetic analysis of the mechanism of interaction of full-length TIMP-2 and gelatinase A: evidence for the existence of a low-affinity intermediate
    M Hutton
    School of Biological Sciences, University of East Anglia, Norwich, UK
    Biochemistry 37:10094-8. 1998
    ..This gives a value for the overall dissociation constant of approximately 0.6 fM...
  12. ncbi request reprint Tyrosine 36 plays a critical role in the interaction of the AB loop of tissue inhibitor of metalloproteinases-2 with matrix metalloproteinase-14
    R A Williamson
    School of Biological Sciences, University of East Anglia, Norwich NR4 7TJ, United Kingdom
    J Biol Chem 276:32966-70. 2001
    ..Mutants of TIMP-2 with more directed specificity may be of use in gene therapeutic approaches to human disease...
  13. ncbi request reprint Immunolocalization of matrix metalloproteinases in partial-thickness defects in pig articular cartilage. A preliminary report
    R M Hembry
    School of Biological Sciences, University of East Anglia, Norwich, United Kingdom
    J Bone Joint Surg Am 83:826-38. 2001
    ..To determine this sequence, we studied the distribution of matrix metalloproteinases and their inhibitors during early in vivo repair of partial-thickness defects in pig articular cartilage...
  14. ncbi request reprint Membrane type 1 matrix metalloproteinase and gelatinase A synergistically degrade type 1 collagen in a cell model
    S J Atkinson
    School of Biological Sciences, University of East Anglia, NR4 7TJ, Norwich, UK
    FEBS Lett 491:222-6. 2001
    ..The study demonstrated that synergy between two non-conventional collagenases effectively degrades insoluble pericellular collagen. Due to the membrane localisation of MT1 MMP, this could potentially occur in a highly localised manner...
  15. ncbi request reprint Shedding of c-Met is regulated by crosstalk between a G-protein coupled receptor and the EGF receptor and is mediated by a TIMP-3 sensitive metalloproteinase
    D Nath
    School of Biological Sciences, University of East Anglia, Norwich NR4 7TJ, UK
    J Cell Sci 114:1213-20. 2001
    ..Our results indicate that ectodomain shedding is a highly regulated process that can be stimulated by EGFR signalling pathways and integrin ligation...
  16. ncbi request reprint Membrane type 4 matrix metalloproteinase (MMP17) has tumor necrosis factor-alpha convertase activity but does not activate pro-MMP2
    W R English
    School of Biological Sciences, University of East Anglia, University Plain, Norwich, Norfolk NR4 7TJ, United Kingdom
    J Biol Chem 275:14046-55. 2000
    ..MT4-MMP was detected by Western blot in monocyte/macrophage cell lines which in combination with its fibrinolytic and TNFalpha-converting activity suggests a role in inflammation...
  17. ncbi request reprint Perivascular cells regulate endothelial membrane type-1 matrix metalloproteinase activity
    M A Lafleur
    School of Biological Sciences, University of East Anglia, Norwich, NR4 7TJ, England
    Biochem Biophys Res Commun 282:463-73. 2001
    ..This interplay may be important for maintenance of blood vessel architecture and neovascularisation...
  18. ncbi request reprint Specific collagenolysis by gelatinase A, MMP-2, is determined by the hemopexin domain and not the fibronectin-like domain
    M L Patterson
    School of Biological Sciences, University of East Anglia, Norwich, NR4 7TJ, UK
    FEBS Lett 503:158-62. 2001
    ..The mechanism of MMP-2 cleavage of collagen proceeds in two phases, the first resembling that of the interstitial collagenases, followed by gelatinolysis, promoted by the fibronectin-like domain...
  19. ncbi request reprint Human tissue inhibitor of metalloproteinases 3 interacts with both the N- and C-terminal domains of gelatinases A and B. Regulation by polyanions
    G S Butler
    School of Biological Sciences, University of East Anglia, Norwich, Norfolk NR4 7TJ, United Kingdom
    J Biol Chem 274:10846-51. 1999
    ..Because TIMP-3 is sequestered in the extracellular matrix, the presence of certain heparan sulfate proteoglycans could enhance its inhibitory capacity...
  20. ncbi request reprint Cellular strategies for proteolytic targeting during migration and invasion
    V Ellis
    School of Biological Sciences, University of East Anglia, Norwich NR4 7TJ, UK
    FEBS Lett 506:1-5. 2001
    ..This mini review reflects the status of our knowledge of strategies for the localisation of proteolytic activity effected during cell migration...
  21. ncbi request reprint TNF-alpha converting enzyme (TACE) is inhibited by TIMP-3
    A Amour
    School of Biological Sciences, University of East Anglia, Norwich, UK
    FEBS Lett 435:39-44. 1998
    ..These results suggest that TIMP-3, unlike the other TIMPs, may be important in the modulation of pathological events in which TNF-alpha secretion is involved...
  22. ncbi request reprint Interaction of metargidin (ADAM-15) with alphavbeta3 and alpha5beta1 integrins on different haemopoietic cells
    D Nath
    School of Biological Sciences, University of East Anglia, Norwich NR4 7TJ, UK
    J Cell Sci 112:579-87. 1999
    ..These results together indicate that metargidin can function as a cell adhesion molecule via interactions with alphavbeta3 and alpha5beta1 integrins...
  23. ncbi request reprint Matrix metalloproteinases at a glance
    Meng Huee Lee
    Dept of Oncology, University of Cambridge, Cambridge Institute for Medical Research, Hills Road, Cambridge CB2 2XY, UK
    J Cell Sci 117:4015-6. 2004
  24. ncbi request reprint Delineating the molecular basis of the inactivity of tissue inhibitor of metalloproteinase-2 against tumor necrosis factor-alpha-converting enzyme
    Meng Huee Lee
    Department of Oncology, Cambridge University, Cambridge Institute for Medical Research, Wellcome Trust Medical Research Council Building, Cambridge CB2 2XY, United Kingdom
    J Biol Chem 279:45121-9. 2004
    ..Our findings here represent a significant advance toward designing tailor-made TIMPs for specific MP targeting...
  25. ncbi request reprint Identification of the extracellular matrix (ECM) binding motifs of tissue inhibitor of metalloproteinases (TIMP)-3 and effective transfer to TIMP-1
    Meng Huee Lee
    Department of Oncology, Cancer Research UK Cambridge Research Institute, Cambridge University, Cambridge CB2 0RE, United Kingdom
    J Biol Chem 282:6887-98. 2007
    ..This is the first instance of TIMPs being intentionally rendered soluble or ECM-bound. The ability to prepare TIMPs in soluble or ECM-bound forms also opens new avenues for future TIMP research...
  26. ncbi request reprint TIMP-3 inhibition of ADAMTS-4 (Aggrecanase-1) is modulated by interactions between aggrecan and the C-terminal domain of ADAMTS-4
    Gareth J Wayne
    GlaxoSmithKline, New Frontiers Research Park, Harlow, Essex CM19 5AW, United Kingdom
    J Biol Chem 282:20991-8. 2007
    ..The results of this study therefore indicate that the cartilage environment can modulate the function of enzyme-inhibitor systems and could have relevance for therapeutic approaches to aggrecanase modulation...
  27. ncbi request reprint Use of a multiple-enzyme/multiple-reagent assay system to quantify activity levels in samples containing mixtures of matrix metalloproteinases
    Fred H Rasmussen
    BioZyme, Inc, Apex, North Carolina 27523, USA
    Biochemistry 43:2987-95. 2004
    ..Some of the best substrates tested have specific activities for collagenase 3 that are 37,000-, 17,000-, 90-, and 200-fold selective over stromelysin 1, collagenase 1, and gelatinases A and B, respectively...
  28. ncbi request reprint Threonine 98, the pivotal residue of tissue inhibitor of metalloproteinases (TIMP)-1 in metalloproteinase recognition
    Meng Huee Lee
    Cambridge Institute for Medical Research, Wellcome Trust Medical Research Council Building, Cambridge University, Cambridge CB2 2XY, United Kingdom
    J Biol Chem 279:17562-9. 2004
    ..Not only have we unlocked the molecular basis for the inactivity of TIMP-1 against several of the MPs, but also our findings fundamentally modify the current beliefs on the molecular mechanism of TIMP-MP recognition and selectivity...
  29. ncbi request reprint Up-regulation of vascular endothelial growth factor-A by active membrane-type 1 matrix metalloproteinase through activation of Src-tyrosine kinases
    Nor Eddine Sounni
    Laboratory of Tumor and Development Biology University of Liège, Sart Tilman, 4000 Sart Tilman, B 4000 Liege, Belgium
    J Biol Chem 279:13564-74. 2004
    ....
  30. ncbi request reprint Temporal and spatial expression of matrix metalloproteinases during wound healing of human corneal tissue
    Julie T Daniels
    Epithelial Repair and Regeneration Group, Wound Healing Research Unit, Division of Pathology, Institute of Ophthalmology, Bath Street, London EC1V 9EL, UK
    Exp Eye Res 77:653-64. 2003
    ..This study demonstrates matrix metalloproteinase expression in epithelial and fibroblast-like cells following wounding of human corneal tissue in culture where the cells remain in contact with their natural matrices...
  31. ncbi request reprint Membrane type I-matrix metalloproteinase (MT1-MMP) is internalised by two different pathways and is recycled to the cell surface
    Albert Remacle
    School of Biological Sciences, University of East Anglia, Norwich NR4 7TJ, UK
    J Cell Sci 116:3905-16. 2003
    ....
  32. ncbi request reprint Characterization of the AB loop region of TIMP-2. Involvement in pro-MMP-2 activation
    Magdalini Rapti
    Department of Oncology, Cambridge University, Cambridge Institute for Medical Research, Wellcome Trust MRC Building, Cambridge CB2 2XY, United Kingdom
    J Biol Chem 281:23386-94. 2006
    ..However, transfer of both the AB loop and C-terminal domain of TIMP-2 to TIMP-4 generates a mutant that can activate pro-MMP-2 and so demonstrates that both these regions of TIMP-2 are important for the activation process...
  33. ncbi request reprint N-i-Propoxy-N-biphenylsulfonylaminobutylhydroxamic acids as potent and selective inhibitors of MMP-2 and MT1-MMP
    Armando Rossello
    Dipartimento di Scienze Farmaceutiche, Universita degli Studi di Pisa, Via Bonanno, 6, 56126 Pisa, Italy
    Bioorg Med Chem Lett 15:1321-6. 2005
    ..The simultaneous inhibition of these two enzymes yields type C compounds, which are potent antiangiogenic agents, able to block a chemoinvasion model on HUVEC cells in the micromolar range...
  34. ncbi request reprint Tumor necrosis factor-alpha-converting enzyme (TACE/ADAM-17) mediates the ectodomain cleavage of intercellular adhesion molecule-1 (ICAM-1)
    Nina L Tsakadze
    Department of Physiology and Biophysics, University of Louisville, Louisville, Kentucky 40292, USA
    J Biol Chem 281:3157-64. 2006
    ....
  35. ncbi request reprint Dissecting the role of matrix metalloproteinases (MMP) and integrin alpha(v)beta3 in angiogenesis in vitro: absence of hemopexin C domain bioactivity, but membrane-Type 1-MMP and alpha(v)beta3 are critical
    Riccardo E Nisato
    Department of Morphology, University Medical Center, Geneva, Switzerland
    Cancer Res 65:9377-87. 2005
    ....
  36. ncbi request reprint Amber force field implementation, molecular modelling study, synthesis and MMP-1/MMP-2 inhibition profile of (R)- and (S)-N-hydroxy-2-(N-isopropoxybiphenyl-4-ylsulfonamido)-3-methylbutanamides
    Tiziano Tuccinardi
    Dipartimento di Scienze Farmaceutiche, Universita di Pisa, Via Bonanno 6, 56126 Pisa, Italy
    Bioorg Med Chem 14:4260-76. 2006
    ....
  37. pmc MT1-MMP hemopexin domain exchange with MT4-MMP blocks enzyme maturation and trafficking to the plasma membrane in MCF7 cells
    Susan J Atkinson
    Department of Oncology, Cambridge University, Cambridge Institute for Medical Research, Hills Road, Cambridge CB2 2XY, UK
    Biochem J 398:15-22. 2006
    ....
  38. ncbi request reprint ERK-mediated phosphorylation of Thr735 in TNFalpha-converting enzyme and its potential role in TACE protein trafficking
    Surinder M Soond
    University of Cambridge, Department of Oncology, Cambridge Institute of Medical Research, Hills Road, Cambridge, CB2 2XY, UK
    J Cell Sci 118:2371-80. 2005
    ..We conclude from our observations that phosphorylation of TACE at Thr735 highlights a key step in inducible TACE protein trafficking and maturation...
  39. ncbi request reprint Membrane type 1-matrix metalloproteinase induces endothelial cell morphogenic differentiation by a caspase-dependent mechanism
    Stephanie Langlois
    Laboratoire de Medecine Moleculaire, Hôpital Ste Justine Université du Québec à Montréal, Centre de cancérologie Charles Bruneau, 3175 Chemin Cote Ste Catherine, Montreal, Quebec, Canada H3T 1C5
    Exp Cell Res 307:452-64. 2005
    ..Our results show that the ability of MT1-MMP to induce EC morphogenic differentiation involves its activation of a caspase-dependent mechanism...
  40. ncbi request reprint Individual Timp deficiencies differentially impact pro-MMP-2 activation
    Jane L English
    Ontario Cancer Institute, 610 University Avenue, Toronto, Ontario M5G 2M9, Canada
    J Biol Chem 281:10337-46. 2006
    ..Thus, TIMP-3 provides an inherent regulation over the kinetics of pro-MMP-2 processing, serving at a level distinct from that of TIMP-2 and TIMP-4...
  41. ncbi request reprint A new development of matrix metalloproteinase inhibitors: twin hydroxamic acids as potent inhibitors of MMPs
    Armando Rossello
    Dipartimento di Scienze Farmaceutiche, Universita degli Studi di Pisa, Via Bonanno, 6, 56126 Pisa, Italy
    Bioorg Med Chem Lett 15:2311-4. 2005
    ....
  42. ncbi request reprint Total conversion of tissue inhibitor of metalloproteinase (TIMP) for specific metalloproteinase targeting: fine-tuning TIMP-4 for optimal inhibition of tumor necrosis factor-{alpha}-converting enzyme
    Meng Huee Lee
    Department of Oncology, Cambridge University, Cambridge Institute for Medical Research, Wellcome Trust MRC Building, Cambridge CB2 2XY, United Kingdom
    J Biol Chem 280:15967-75. 2005
    ..Drawing together our previous experience in TACE-targeted mutagenesis by using TIMP-1 and -2 scaffolds, we have finally resolved the mystery of the selective sensitivity of TACE to TIMP-3...
  43. ncbi request reprint Structure and function of matrix metalloproteinases and TIMPs
    Hideaki Nagase
    Department of Matrix Biology, Kennedy Institute of Rheumatology Division, Faculty of Medicine, Imperial College London, 1 Aspenlea Road, London W6 8LH, UK
    Cardiovasc Res 69:562-73. 2006
    ..This review introduces the members of the MMP family and discusses their domain structure and function, proenyme activation, the mechanism of inhibition by TIMPs and their significance in physiology and pathology...
  44. doi request reprint Reappraising metalloproteinases in rheumatoid arthritis and osteoarthritis: destruction or repair?
    Gillian Murphy
    Cancer Cell Biology, Department of Oncology, University of Cambridge, Cambridge, UK
    Nat Clin Pract Rheumatol 4:128-35. 2008
    ..Work on the development of effective biomarkers is also essential before an effective evaluation of the new generations of specific inhibitors can be made...
  45. ncbi request reprint ADAMTS1 cleaves aggrecan at multiple sites and is differentially inhibited by metalloproteinase inhibitors
    Juan Carlos Rodriguez-Manzaneque
    Department of Molecular, Cell and Developmental Biology, Molecular Biology Institute, University of California at Los Angeles, 611, Charles E Young Drive, Los Angeles, CA 90095, USA
    Biochem Biophys Res Commun 293:501-8. 2002
    ..Finally, we developed an activity assay using an artificial peptide substrate based on the interglobular domain cleavage site (E(373)-A) of rat aggrecan...
  46. ncbi request reprint The enzymatic activity of ADAM8 and ADAM9 is not regulated by TIMPs
    Augustin Amour
    School of Biological Sciences, University of East Anglia, Norwich, UK
    FEBS Lett 524:154-8. 2002
    ....
  47. ncbi request reprint A novel function for tissue inhibitor of metalloproteinases-3 (TIMP3): inhibition of angiogenesis by blockage of VEGF binding to VEGF receptor-2
    Jian Hua Qi
    Department of Ophthalmic Research, Cole Eye Institute, Cleveland Clinic Foundation, Cleveland, Ohio, USA
    Nat Med 9:407-15. 2003
    ..This property seems to be independent of its MMP-inhibitory activity, indicating a new function for this molecule...
  48. pmc Engineering N-terminal domain of tissue inhibitor of metalloproteinase (TIMP)-3 to be a better inhibitor against tumour necrosis factor-alpha-converting enzyme
    Meng Huee Lee
    School of Biological Sciences, University of East Anglia, Norwich NR4 7TJ, U K
    Biochem J 364:227-34. 2002
    ....
  49. pmc Investigation of the role of Endo180/urokinase-type plasminogen activator receptor-associated protein as a collagenase 3 (matrix metalloproteinase 13) receptor
    Louise Bailey
    School of Biological Sciences, University of East Anglia, Norwich NR4 7TJ, UK
    Biochem J 363:67-72. 2002
    ..We conclude that Endo180 is unlikely to be a receptor for collagenase 3 in relation to either its activation or cell binding and internalization, and that other interaction partners must be sought...
  50. ncbi request reprint Tissue inhibitor of metalloproteinase-3 induces a Fas-associated death domain-dependent type II apoptotic pathway
    Mark Bond
    Bristol Heart Institute, Level 7, Bristol Royal Infirmary, University of Bristol, Bristol BS2 8HW, United Kingdom
    J Biol Chem 277:13787-95. 2002
    ..Taken together, these results indicate that TIMP-3 overexpression induces a type II apoptotic pathway initiated via a Fas-associated death domain-dependent mechanism...
  51. ncbi request reprint Metalloproteases cleave the urokinase-type plasminogen activator receptor in the D1-D2 linker region and expose epitopes not present in the intact soluble receptor
    Annapaola Andolfo
    Department of Molecular Pathology and Medicine, Molecular Genetics Unit, DIBIT, San Raffaele Scientific Institute, Milan, Italy
    Thromb Haemost 88:298-306. 2002
    ..Using an antibody raised against the human uPAR linker region we show that this region of uPAR, which contains the chemotactic SRSRY epitope, is exposed upon MMP cleavage...
  52. ncbi request reprint Metalloproteinase inhibitors: biological actions and therapeutic opportunities
    Andrew H Baker
    BHF Blood Pressure Group, Department of Medicine and Therapeutics, Western Infirmary, Glasgow G11 6NT, UK
    J Cell Sci 115:3719-27. 2002
    ....
  53. pmc Mapping and characterization of the functional epitopes of tissue inhibitor of metalloproteinases (TIMP)-3 using TIMP-1 as the scaffold: a new frontier in TIMP engineering
    Meng Huee Lee
    School of Biological Sciences, University of East Anglia, Norwich NR4 7TJ, UK
    Protein Sci 11:2493-503. 2002
    ..Most importantly, our findings confirm that the individual characteristics of TIMP could be transplanted from one variant to another...
  54. ncbi request reprint Cellular activation of proMMP-13 by MT1-MMP depends on the C-terminal domain of MMP-13
    Vera Knauper
    School of Biological Sciences, University of East Anglia, Norwich NR4 7TJ, UK
    FEBS Lett 532:127-30. 2002
    ..Unlike proMMP-2 activation, this process was independent of tissue inhibitor of metalloproteinase-2 (TIMP-2) as MT1-MMP expressing cells from the TIMP-2-/- mouse efficiently activated proMMP-13...
  55. pmc Tailoring tissue inhibitor of metalloproteinases-3 to overcome the weakening effects of the cysteine-rich domains of tumour necrosis factor-alpha converting enzyme
    Meng Huee Lee
    School of Biological Sciences, University of East Anglia, Norwich, NR4 7TJ, UK
    Biochem J 371:369-76. 2003
    ..Further expression of one of the mutants, Lys(26/27/30/76)-->Glu, in a mammalian expression system confirmed that TIMP-3 associates with the extracellular matrix via its C-terminal domain...
  56. ncbi request reprint Sorsby's fundus dystrophy tissue inhibitor of metalloproteinases-3 (TIMP-3) mutants have unimpaired matrix metalloproteinase inhibitory activities, but affect cell adhesion to the extracellular matrix
    Karen M Yeow
    School of Biological Sciences, University of East Anglia, Norwich, Norfolk NR4 7TJ, UK
    Matrix Biol 21:75-88. 2002
    ....
  57. pmc Sequence motifs of tissue inhibitor of metalloproteinases 2 (TIMP-2) determining progelatinase A (proMMP-2) binding and activation by membrane-type metalloproteinase 1 (MT1-MMP)
    Joanna R Worley
    School of Biological Sciences, University of East Anglia, Norwich NR4 7TJ, UK
    Biochem J 372:799-809. 2003
    ....
  58. ncbi request reprint Phosphorylation-dependent interactions between ADAM15 cytoplasmic domain and Src family protein-tyrosine kinases
    Zaruhi Poghosyan
    School of Biological Sciences, University of East Anglia, Norwich NR4 7TJ, United Kingdom
    J Biol Chem 277:4999-5007. 2002
    ..These data demonstrate selective, phosphorylation-dependent interactions of ADAM15 with Src family PTKs and Grb2, which highlight the potential for integration of ADAM functions and cellular signaling...
  59. ncbi request reprint Unveiling the surface epitopes that render tissue inhibitor of metalloproteinase-1 inactive against membrane type 1-matrix metalloproteinase
    Meng Huee Lee
    Cambridge Institute for Medical Research, Wellcome Trust MRC Building, Cambridge University, Cambridge CB2 2XY, United Kingdom
    J Biol Chem 278:40224-30. 2003
    ..Our findings suggest that threonine 98 is critical in initiating MMP binding and complex stabilization. Our findings also provide a potential mechanistic explanation for MMP-TIMP selectivity...
  60. ncbi request reprint The C-terminal domains of TACE weaken the inhibitory action of N-TIMP-3
    Meng Huee Lee
    School of Biological Sciences, University of East Anglia, Norwich NR4 7TJ, UK
    FEBS Lett 520:102-6. 2002
    ..Our findings highlight the potential role of the C-terminal domains of ADAM proteinases in influencing TIMP interactions...
  61. doi request reprint The soluble form of a disintegrin and metalloprotease 33 promotes angiogenesis: implications for airway remodeling in asthma
    Ilaria Puxeddu
    Division of Infection, Inflammation, and Repair, School of Medicine, Southampton General Hospital, University of Southampton, Southampton, United Kingdom
    J Allergy Clin Immunol 121:1400-6, 1406.e1-4. 2008
    ..A disintegrin and metalloprotease (ADAM)-33 is a susceptibility gene for asthma and chronic obstructive pulmonary disease whose function remains unknown...
  62. ncbi request reprint Membrane-type 1-matrix metalloproteinase regulates intracellular adhesion molecule-1 (ICAM-1)-mediated monocyte transmigration
    Srinivas D Sithu
    Department of Physiology and Biophysics, University of Louisville, Louisville, Kentucky 40202, USA
    J Biol Chem 282:25010-9. 2007
    ..MT1-MMP localization with cells expressing ICAM-1 mutations was reduced and diffused. These results indicate that the cytoplasmic tail of ICAM-1 regulates leukocyte transmigration through MT1-MMP interaction...
  63. doi request reprint The isolated N-terminal domains of TIMP-1 and TIMP-3 are insufficient for ADAM10 inhibition
    Magdalini Rapti
    Department of Oncology, Cambridge University, Cancer Research UK Cambridge Research Institute, Li Ka Shing Centre, Cambridge CB2 0RE, UK
    Biochem J 411:433-9. 2008
    ..This knowledge could form the basis for the design of directed inhibitors against different metalloproteinases...
  64. pmc Substrate selectivity of epidermal growth factor-receptor ligand sheddases and their regulation by phorbol esters and calcium influx
    Keisuke Horiuchi
    Arthritis and Tissue Degeneration Program, Hospital for Special Surgery, New York, NY 10021, USA
    Mol Biol Cell 18:176-88. 2007
    ....
  65. doi request reprint Clipping, shedding and RIPping keep immunity on cue
    Gillian Murphy
    Department of Oncology, University of Cambridge and Cancer Research UK Cambridge Institute, Cambridge, UK
    Trends Immunol 29:75-82. 2008
    ....
  66. ncbi request reprint Granulocyte-macrophage colony stimulating factor induces endothelial capillary formation through induction of membrane-type 1 matrix metalloproteinase expression in vitro
    Davia Krubasik
    Cancer Research UK, Cambridge Research Institute, Cambridge, United Kingdom
    Int J Cancer 122:1261-72. 2008
    ....
  67. ncbi request reprint Membrane-type 4 matrix metalloproteinase promotes breast cancer growth and metastases
    Vincent Chabottaux
    Laboratory of Tumor and Development Biology, Centre de Recherche en cancérologie Expérimentale, Center for Biomedical Integrative Genoproteomics, University of Liege, Belgium
    Cancer Res 66:5165-72. 2006
    ..t. blood vessels, and is associated with increased lung metastases. These results identify MT4-MMP as a new putative target to design anticancer strategies...
  68. pmc Increased matrix metalloproteinase-13 production with aging by human articular chondrocytes in response to catabolic stimuli
    Christopher B Forsyth
    Department of Internal Medicine, Rush Medical College, Rush University Medical Center, Chicago, Illinois, USA
    J Gerontol A Biol Sci Med Sci 60:1118-24. 2005
    ..These results demonstrate a significant age-related increase in chondrocyte catabolic responsiveness which could contribute to the development of osteoarthritis in older adults...
  69. ncbi request reprint Endothelial tubulogenesis within fibrin gels specifically requires the activity of membrane-type-matrix metalloproteinases (MT-MMPs)
    Marc A Lafleur
    School of Biological Sciences, University of East Anglia, Norwich, NR4 7TJ, UK
    J Cell Sci 115:3427-38. 2002
    ..Therefore MT-MMPs and specifically MT1-MMP are likely candidates for involvement during endothelial tubulogenesis within a fibrin matrix, and thus their blockade may be a viable strategy for inhibition of angiogenesis...
  70. ncbi request reprint Differential expression of matrix metalloproteinases during impaired wound healing of the diabetes mouse
    Steven J Wall
    School of Biological Sciences, University of East Anglia, Norwich, U K
    J Invest Dermatol 119:91-8. 2002
    ....
  71. ncbi request reprint Human corneal epithelial cells require MMP-1 for HGF-mediated migration on collagen I
    Julie T Daniels
    Epithelial Repair and Regeneration Group, Wound Healing Research Unit, Division of Pathology, Institute of Ophthalmology, London, United Kingdom
    Invest Ophthalmol Vis Sci 44:1048-55. 2003
    ..To investigate the potential regulation of matrix metalloproteinases (MMPs) by hepatocyte growth factor (HGF), and to identify individual MMPs essential for migration of human corneal epithelial cells...
  72. ncbi request reprint Measurement of the noncomplexed free fraction of tissue inhibitor of metalloproteinases 1 in plasma by immunoassay
    Mads N Holten-Andersen
    The Finsen Laboratory, Rigshopitalet, Strandboulevarden 49, 2100 Copenhagen, Denmark
    Clin Chem 48:1305-13. 2002
    ..Because TIMP-1 can exist in more than one molecular form, a new immunoassay to specifically detect free TIMP-1 was developed and concentrations were determined in plasma from healthy donors and colorectal cancer (CRC) patients...
  73. pmc Human diabetic corneas preserve wound healing, basement membrane, integrin and MMP-10 differences from normal corneas in organ culture
    Andrea Kabosova
    Ophthalmology Research Laboratories, Cedars Sinai Medical Center, Burns and Allen Research Institute, Los Angeles, CA 90048, USA
    Exp Eye Res 77:211-7. 2003
    ..The established corneal organ culture provides an adequate system for elucidating mechanisms of epithelial alterations in human DR corneas...
  74. ncbi request reprint New N-arylsulfonyl-N-alkoxyaminoacetohydroxamic acids as selective inhibitors of gelatinase A (MMP-2)
    Armando Rossello
    Dipartimento di Scienze Farmaceutiche, Universita degli Studi di Pisa, Via Bonanno, 6, 56126 Pisa, Italy
    Bioorg Med Chem 12:2441-50. 2004
    ....
  75. ncbi request reprint Cellular cholesterol regulates MT1 MMP dependent activation of MMP 2 via MEK-1 in HT1080 fibrosarcoma cells
    Susan J Atkinson
    Department of Oncology, Cambridge Institute for Medical Research, University of Cambridge, Cambridge CB2 2XY, UK
    FEBS Lett 566:65-70. 2004
    ..Our data indicate that the cholesterol content of unstimulated cells is critical for secretion of MMP 2 as an inactive zymogen and control of pericellular proteolysis...
  76. ncbi request reprint ADAM 33 and its association with airway remodeling and hyperresponsiveness in asthma
    Stephen T Holgate
    Respiratory Cell and Molecular Biology Research Division, School of Medicine, University of Southampton, UK, University of Cambridge, UK
    Clin Rev Allergy Immunol 27:23-34. 2004
    ..The potential for therapeutic intervention with ADAM 33 is extremely attractive and further work will not only focus on the specific domains of ADAM 33, but also the mechanisms by which they lead to bronchial hyperreactivity...
  77. ncbi request reprint Src-mediated tyrosine phosphorylation of caveolin-1 induces its association with membrane type 1 matrix metalloproteinase
    Lyne Labrecque
    Laboratoire de Medecine Moleculaire, Hôpital Ste Justine Université du Québec à Montréal, Centre de cancérologie Charles Bruneau, 3175 Chemin Cote Ste Catherine, Montreal, Quebec H3T 1C5
    J Biol Chem 279:52132-40. 2004
    ..Overall these results indicate that MT1-MMP associates with tyrosine-phosphorylated caveolin-1 and that this complex may play an important role in MT1-MMP regulation and function...
  78. pmc Matrix metalloproteinase-1 associates with intracellular organelles and confers resistance to lamin A/C degradation during apoptosis
    G Astrid Limb
    Department of Pathology, Institute of Ophthalmology, University College London and Moorfields Eye Hospital, 11 Bath St, London EC1V 9EL
    Am J Pathol 166:1555-63. 2005
    ..These observations strongly suggest that intracellular association of MMP-1 to mitochondria and nuclei confers resistance to apoptosis and may explain the well-known association of this enzyme with tumor cell survival and spreading...
  79. ncbi request reprint Cytokine stimulated vascular cell adhesion molecule-1 (VCAM-1) ectodomain release is regulated by TIMP-3
    Robert J R Singh
    School of Biological Sciences, University of East Anglia, Norwich, NR4 7TJ, UK
    Cardiovasc Res 67:39-49. 2005
    ..Here we investigate mechanisms involved in sVCAM-1 generation in response to cytokine stimulation...
  80. ncbi request reprint Matrix metalloproteinases in disease and repair processes in the anterior segment
    Tina T L Wong
    Wound Healing Research Unit, Department of Pathology, Institute of Ophthalmology and Moorfields Eye Hospital, London, UK
    Surv Ophthalmol 47:239-56. 2002
    ..The importance of MMPs in the wound healing response and its potential modulation to manipulate the scarring response is being recognized, and current developments will be described...