Sean Munro

Summary

Affiliation: University of Cambridge
Country: UK

Publications

  1. ncbi request reprint Organelle identity and the signposts for membrane traffic
    Rudy Behnia
    MRC Laboratory of Molecular Biology, Hills Road, Cambridge CB2 2QH, UK
    Nature 438:597-604. 2005
  2. pmc A comprehensive comparison of transmembrane domains reveals organelle-specific properties
    Hayley J Sharpe
    MRC Laboratory of Molecular Biology, Hills Road, Cambridge CB2 0QH, UK
    Cell 142:158-69. 2010
  3. pmc Arl8 and SKIP act together to link lysosomes to kinesin-1
    Cláudia Rosa-Ferreira
    MRC Laboratory of Molecular Biology, Hills Road, Cambridge CB2 0QH, UK
    Dev Cell 21:1171-8. 2011
  4. pmc Golgi coiled-coil proteins contain multiple binding sites for Rab family G proteins
    Rita Sinka
    Medical Research Council Laboratory of Molecular Biology, Cambridge, England, UK
    J Cell Biol 183:607-15. 2008
  5. pmc The small G protein Arl1 directs the trans-Golgi-specific targeting of the Arf1 exchange factors BIG1 and BIG2
    Chantal Christis
    Medical Research Council Laboratory of Molecular Biology, Cambridge CB2 0QH, England, UK
    J Cell Biol 196:327-35. 2012
  6. pmc Membrane delivery to the yeast autophagosome from the Golgi-endosomal system
    Yohei Ohashi
    Medical Research Council Laboratory of Molecular Biology, Cambridge CB2 0QH, UK
    Mol Biol Cell 21:3998-4008. 2010
  7. pmc The GTPase Arf1p and the ER to Golgi cargo receptor Erv14p cooperate to recruit the golgin Rud3p to the cis-Golgi
    Alison K Gillingham
    Medical Research Council Laboratory of Molecular Biology, Cambridge CB2 2QH, England, UK
    J Cell Biol 167:281-92. 2004
  8. pmc The exocyst component Sec5 is present on endocytic vesicles in the oocyte of Drosophila melanogaster
    Bernhard Sommer
    Medical Research Council Laboratory of Molecular Biology, Cambridge CB2 2QH, England, UK
    J Cell Biol 169:953-63. 2005
  9. pmc A systematic approach to pair secretory cargo receptors with their cargo suggests a mechanism for cargo selection by Erv14
    Yonatan Herzig
    Department of Molecular Genetics, Weizmann Institute of Science, Rehovot, Israel
    PLoS Biol 10:e1001329. 2012
  10. ncbi request reprint Long coiled-coil proteins and membrane traffic
    Alison K Gillingham
    MRC Laboratory of Molecular Biology, Hills Road, Cambridge, CB2 2QH, UK
    Biochim Biophys Acta 1641:71-85. 2003

Collaborators

Detail Information

Publications53

  1. ncbi request reprint Organelle identity and the signposts for membrane traffic
    Rudy Behnia
    MRC Laboratory of Molecular Biology, Hills Road, Cambridge CB2 2QH, UK
    Nature 438:597-604. 2005
    ..Recent studies have helped to establish how cells maintain and restrict these determinants and explain how this system is exploited by invading pathogens...
  2. pmc A comprehensive comparison of transmembrane domains reveals organelle-specific properties
    Hayley J Sharpe
    MRC Laboratory of Molecular Biology, Hills Road, Cambridge CB2 0QH, UK
    Cell 142:158-69. 2010
    ..The pervasive presence of organelle-specific features among the TMDs of a particular organelle has implications for TMD prediction, regulation of protein activity by location, and sorting of proteins and lipids in the secretory pathway...
  3. pmc Arl8 and SKIP act together to link lysosomes to kinesin-1
    Cláudia Rosa-Ferreira
    MRC Laboratory of Molecular Biology, Hills Road, Cambridge CB2 0QH, UK
    Dev Cell 21:1171-8. 2011
    ..A splice variant of SKIP that lacks a light chain binding motif does not stimulate movement, suggesting fine-tuning by alternative splicing...
  4. pmc Golgi coiled-coil proteins contain multiple binding sites for Rab family G proteins
    Rita Sinka
    Medical Research Council Laboratory of Molecular Biology, Cambridge, England, UK
    J Cell Biol 183:607-15. 2008
    ..Binding sites for diverse Rabs could ensure that incoming carriers are captured on first contact and moved to their correct destination within the stack...
  5. pmc The small G protein Arl1 directs the trans-Golgi-specific targeting of the Arf1 exchange factors BIG1 and BIG2
    Chantal Christis
    Medical Research Council Laboratory of Molecular Biology, Cambridge CB2 0QH, England, UK
    J Cell Biol 196:327-35. 2012
    ..We show that in mammalian cells, Arl1 is necessary for Golgi recruitment of BIG1 and BIG2 but not GBF1. Thus, Arl1 acts to direct a trans-Golgi-specific Arf1 GEF, and hence active Arf1, to the trans side of the Golgi...
  6. pmc Membrane delivery to the yeast autophagosome from the Golgi-endosomal system
    Yohei Ohashi
    Medical Research Council Laboratory of Molecular Biology, Cambridge CB2 0QH, UK
    Mol Biol Cell 21:3998-4008. 2010
    ..This is underestimated by examination of single mutants, providing a possible explanation for discrepancies between yeast and mammalian studies on Atg9 localization and autophagosome formation...
  7. pmc The GTPase Arf1p and the ER to Golgi cargo receptor Erv14p cooperate to recruit the golgin Rud3p to the cis-Golgi
    Alison K Gillingham
    Medical Research Council Laboratory of Molecular Biology, Cambridge CB2 2QH, England, UK
    J Cell Biol 167:281-92. 2004
    ..Tassin, C. Fedriani, and M. Bornens. 2004. Cell. 118:323-335). In contrast, we find that this region binds to the Golgi in a GRAB domain-dependent manner, suggesting that GMAP-210 may not link the Golgi to gamma-tubulin and centrosomes...
  8. pmc The exocyst component Sec5 is present on endocytic vesicles in the oocyte of Drosophila melanogaster
    Bernhard Sommer
    Medical Research Council Laboratory of Molecular Biology, Cambridge CB2 2QH, England, UK
    J Cell Biol 169:953-63. 2005
    ..Thus, the exocyst may act in both Golgi to plasma membrane traffic and endocytic cycling, and hence in oocytes is recruited to clathrin-coated pits to facilitate the rapid recycling of Yolkless...
  9. pmc A systematic approach to pair secretory cargo receptors with their cargo suggests a mechanism for cargo selection by Erv14
    Yonatan Herzig
    Department of Molecular Genetics, Weizmann Institute of Science, Rehovot, Israel
    PLoS Biol 10:e1001329. 2012
    ..Such a mechanism would allow diverse proteins to utilize a few receptors without the constraints of evolving location-specific sorting motifs...
  10. ncbi request reprint Long coiled-coil proteins and membrane traffic
    Alison K Gillingham
    MRC Laboratory of Molecular Biology, Hills Road, Cambridge, CB2 2QH, UK
    Biochim Biophys Acta 1641:71-85. 2003
    ..This review will examine what is known about the function of these large coiled-coil proteins in membrane traffic...
  11. ncbi request reprint Organelle identity and the targeting of peripheral membrane proteins
    Sean Munro
    MRC Laboratory of Molecular Biology, Hills Road, Cambridge CB2 2QH, UK
    Curr Opin Cell Biol 14:506-14. 2002
    ..This suggests that these determinants are what specify organelle identity, and raises interesting questions about how they are generated in an organelle-specific fashion...
  12. ncbi request reprint The Golgi apparatus: defining the identity of Golgi membranes
    Sean Munro
    MRC Laboratory of Molecular Biology, Hills Road, Cambridge CB2 2QH, UK
    Curr Opin Cell Biol 17:395-401. 2005
    ..Recent work is starting to reveal the mechanisms by which these labile landmarks are generated in a spatially restricted manner on specific parts of the Golgi...
  13. ncbi request reprint The Arf-like GTPase Arl1 and its role in membrane traffic
    S Munro
    MRC Laboratory of Molecular Biology, Hills Road, Cambridge CB2 2QH, UK
    Biochem Soc Trans 33:601-5. 2005
    ..Genetic analysis in a number of species has shown that Arl1 is not essential for exocytosis, but rather suggest that it is required for traffic from endosomes to the Golgi...
  14. ncbi request reprint Organelle identity and the organization of membrane traffic
    Sean Munro
    MRC Laboratory of Molecular Biology, Hills Road, Cambridge CB2 2QH, UK
    Nat Cell Biol 6:469-72. 2004
    ..These recruiting determinants are then recognized by cytosolic proteins that facilitate the formation and delivery of vesicles at the correct compartment...
  15. ncbi request reprint Lipid rafts: elusive or illusive?
    Sean Munro
    MRC Laboratory of Molecular Biology, Hills Road, Cambridge CB2 2QH, United Kingdom
    Cell 115:377-88. 2003
    ....
  16. ncbi request reprint What can yeast tell us about N-linked glycosylation in the Golgi apparatus?
    S Munro
    Medical Research Council Laboratory of Molecular Biology, Hills Road, CB2 2QH, Cambridge, UK
    FEBS Lett 498:223-7. 2001
    ..This review discusses the potential of the yeast system to address these issues...
  17. ncbi request reprint The notch signalling regulator fringe acts in the Golgi apparatus and requires the glycosyltransferase signature motif DXD
    S Munro
    MRC Laboratory of Molecular Biology, Cambridge, CB2 2QH, UK
    Curr Biol 10:813-20. 2000
    ..It also has distant homology to bacterial glycosyltransferases, although it is not clear if this reflects a shared enzymatic activity, or merely a related structure...
  18. ncbi request reprint Localization of proteins to the Golgi apparatus
    S Munro
    MRC Laboratory of Molecular Biology, Cambridge, UK
    Trends Cell Biol 8:11-5. 1998
    ..Consideration of how these targeting domains could function has provided insights into the organization of the Golgi and its protein and lipid content...
  19. ncbi request reprint Targeting of the Arf-like GTPase Arl3p to the Golgi requires N-terminal acetylation and the membrane protein Sys1p
    Rudy Behnia
    MRC Laboratory of Molecular Biology, Hills Road, Cambridge CB2 2QH, UK
    Nat Cell Biol 6:405-13. 2004
    ..This suggests that the targeting of ARFRP1/Arl3p to the Golgi is mediated by a direct interaction between its acetylated N terminus and Sys1p/hSys1...
  20. ncbi request reprint Mon2, a relative of large Arf exchange factors, recruits Dop1 to the Golgi apparatus
    Alison K Gillingham
    Medical Research Council Laboratory of Molecular Biology, Hills Road, Cambridge CB2 2QH, United Kingdom
    J Biol Chem 281:2273-80. 2006
    ..Thus, it appears that Dop1 plays a widespread role in membrane organization, and Mon2 acts as a scaffold to recruit the Golgi-localized pool of Dop1...
  21. pmc The yeast orthologue of GRASP65 forms a complex with a coiled-coil protein that contributes to ER to Golgi traffic
    Rudy Behnia
    Laboratory of Molecular Biology, Medical Research Council, Cambridge CB2 2QH, England, UK
    J Cell Biol 176:255-61. 2007
    ....
  22. ncbi request reprint An N-acetylglucosaminyltransferase of the Golgi apparatus of the yeast Saccharomyces cerevisiae that can modify N-linked glycans
    Takehiko Yoko-o
    MRC Laboratory of Molecular Biology, Hills Road, Cambridge CB2 2QH, UK
    Glycobiology 13:581-9. 2003
    ..In addition, they indicate that the lumen of the yeast Golgi contains UDP-GlcNAc, which may facilitate reconstitution of higher eukaryotic N-glycan processing...
  23. ncbi request reprint Structural basis for Arl1-dependent targeting of homodimeric GRIP domains to the Golgi apparatus
    Bojana Panic
    MRC Laboratory of Molecular Biology, MRC Centre, Hills Road, Cambridge CB2 2QH, United Kingdom
    Mol Cell 12:863-74. 2003
    ..Despite no conservation of sequence, topology, or even helical direction, several other effectors form similar interactions with small GTPases via a pair of alpha helices, suggesting a common structural basis for effector recognition...
  24. pmc CASP, the alternatively spliced product of the gene encoding the CCAAT-displacement protein transcription factor, is a Golgi membrane protein related to giantin
    Alison K Gillingham
    MRC Laboratory of Molecular Biology, Cambridge CB2 2QH, United Kingdom
    Mol Biol Cell 13:3761-74. 2002
    ..The conserved histidine is necessary for Coy1p's activity in cells lacking Gos1p, suggesting that the TMD of these transmembrane Golgi coiled-coil proteins is directly involved in their function...
  25. ncbi request reprint An N-terminally acetylated Arf-like GTPase is localised to lysosomes and affects their motility
    Irmgard Hofmann
    MRC Laboratory of Molecular Biology, Hills Road, Cambridge, CB2 2QH, UK
    J Cell Sci 119:1494-503. 2006
    ..Live cell imaging shows that lysosomes move more frequently both toward and away from the cell periphery, suggesting a role for Arl8a and Arl8b as positive regulators of lysosomal transport...
  26. pmc Identification of a guanine nucleotide exchange factor for Arf3, the yeast orthologue of mammalian Arf6
    Alison K Gillingham
    Medical Research Council Laboratory of Molecular Biology, Cambridge, United Kingdom
    PLoS ONE 2:e842. 2007
    ..Moreover the Sec7 domain of Yel1 is required for its membrane targeting and for that of Arf3. Finally we show that the isolated Yel1 Sec7 domain strongly stimulates nucleotide exchange activity specifically on Arf3 in vitro...
  27. ncbi request reprint The ARF-like GTPases Arl1p and Arl3p act in a pathway that interacts with vesicle-tethering factors at the Golgi apparatus
    Bojana Panic
    MRC Laboratory of Molecular Biology, Hills Road, Cambridge CB2 2QH, United Kingdom
    Curr Biol 13:405-10. 2003
    ..Finally, we find that Arl3p is required in vivo for the targeting of Arl1p, explaining its requirement for the normal distribution of Imh1p...
  28. ncbi request reprint The components of the Saccharomyces cerevisiae mannosyltransferase complex M-Pol I have distinct functions in mannan synthesis
    Jurgen Stolz
    MRC Laboratory of Molecular Biology, Hills Road, Cambridge CB2 2QH, United Kingdom
    J Biol Chem 277:44801-8. 2002
    ..Finally, we show that a simple glycoprotein based on hen egg lysozyme can be used as a substrate for modification by purified M-Pol I in vitro...
  29. ncbi request reprint Selective export of HLA-F by its cytoplasmic tail
    Louise H Boyle
    Department of Pathology, University of Cambridge, Cambridge, United Kingdom
    J Immunol 176:6464-72. 2006
    ..This divergent trafficking of HLA-F implicates an alternative function for HLA-F, independent of loading with peptides in the ER...
  30. ncbi request reprint The Arl4 family of small G proteins can recruit the cytohesin Arf6 exchange factors to the plasma membrane
    Irmgard Hofmann
    MRC Laboratory of Molecular Biology, Hills Road, Cambridge CB2 2QH, United Kingdom
    Curr Biol 17:711-6. 2007
    ..The Arl4 family thus defines a signal-transduction pathway that can mediate the plasma-membrane recruitment of cytohesins independently of a requirement for the generation of PtdIns(3,4,5)P(3)...
  31. pmc The PACT domain, a conserved centrosomal targeting motif in the coiled-coil proteins AKAP450 and pericentrin
    A K Gillingham
    MRC Laboratory of Molecular Biology, Cambridge, UK
    EMBO Rep 1:524-9. 2000
    ..When isolated from transfected cells the C-terminal domain of AKAP450 was associated with calmodulin, suggesting that this protein could contribute to centrosome assembly...
  32. pmc Dual targeting of Osh1p, a yeast homologue of oxysterol-binding protein, to both the Golgi and the nucleus-vacuole junction
    T P Levine
    MRC Laboratory of Molecular Biology, Cambridge CB2 2QH, United Kingdom
    Mol Biol Cell 12:1633-44. 2001
    ..GFP fusions to Osh2p and Osh3p showed intracellular distributions distinct from that of Osh1p, and their PH domains appear to contribute to their differing localizations...
  33. ncbi request reprint Vesicle tethering complexes in membrane traffic
    James R C Whyte
    MRC Laboratory of Molecular Biology, Hills Road, Cambridge CB2 2QH, UK
    J Cell Sci 115:2627-37. 2002
    ..Moreover, the diversity of tethering complexes in the cell suggests that not all tethering events occur through the same mechanisms...
  34. pmc An investigation of the role of transmembrane domains in Golgi protein retention
    S Munro
    MRC Laboratory of Molecular Biology, Cambridge, UK
    EMBO J 14:4695-704. 1995
    ..These observations are more consistent with lipid-based sorting of ST TMD, but they also allow for reconciliation with the kin recognition model which appears to act on sequences outside of the TMD...
  35. ncbi request reprint The small G proteins of the Arf family and their regulators
    Alison K Gillingham
    MRC Laboratory of Molecular Biology, Cambridge, United Kingdom
    Annu Rev Cell Dev Biol 23:579-611. 2007
    ..Here we review what is known about all the members of the Arf family, along with the known regulatory molecules that convert them between GDP- and GTP-bound states...
  36. ncbi request reprint The GRIP domain - a novel Golgi-targeting domain found in several coiled-coil proteins
    S Munro
    MRC Laboratory of Molecular Biology, Hills Road, Cambridge CB2 2QH
    Curr Biol 9:377-80. 1999
    ..This result suggests that this small domain functions to recruit specific coiled-coil proteins to the Golgi by recognising a determinant that has been well conserved in eukaryotic evolution...
  37. pmc Multi-protein complexes in the cis Golgi of Saccharomyces cerevisiae with alpha-1,6-mannosyltransferase activity
    J Jungmann
    MRC Laboratory of Molecular Biology, Cambridge, UK
    EMBO J 17:423-34. 1998
    ..Our data suggest that these two multi-protein complexes are responsible for the synthesis and initial branching of the long alpha-1,6-linked backbone of the hypermannose structure attached to many yeast glycoproteins...
  38. ncbi request reprint A yeast homolog of the mammalian mannose 6-phosphate receptors contributes to the sorting of vacuolar hydrolases
    J R Whyte
    Medical Research Council Laboratory of Molecular Biology, Hills Road, CB2 2QH, Cambridge, United Kingdom
    Curr Biol 11:1074-8. 2001
    ..Moreover, there is a strong synergistic effect on the maturation of proteinases A and B when both MRL1 and VPS10 are deleted, which suggests that Mrl1p may serve as a sorting receptor in the delivery of vacuolar hydrolases...
  39. ncbi request reprint Identification of the MNN2 and MNN5 mannosyltransferases required for forming and extending the mannose branches of the outer chain mannans of Saccharomyces cerevisiae
    J C Rayner
    Medical Research Council Laboratory of Molecular Biology, Hills Road, Cambridge CB2 2QH, United Kingdom
    J Biol Chem 273:26836-43. 1998
    ..The identification of Mnn2p and Mnn5p allows us to assign Golgi proteins to all of the catalytic steps in S. cerevisiae mannan synthesis...
  40. pmc The functioning of the yeast Golgi apparatus requires an ER protein encoded by ANP1, a member of a new family of genes affecting the secretory pathway
    R E Chapman
    MRC Laboratory of Molecular Biology, Cambridge, UK
    EMBO J 13:4896-907. 1994
    ..Interestingly, Anp1p is localized to the endoplasmic reticulum (ER), implying that some function of the ER is required to maintain a functional Golgi apparatus...
  41. ncbi request reprint The pleckstrin homology domain of oxysterol-binding protein recognises a determinant specific to Golgi membranes
    T P Levine
    MRC Laboratory of Molecular Biology, Cambridge, UK
    Curr Biol 8:729-39. 1998
    ..It does, however, contain a pleckstrin homology (PH) domain, which in other proteins has been found to mediate regulated membrane binding, although in all previously studied examples the binding is to the plasma membrane...
  42. ncbi request reprint The Sec34/35 Golgi transport complex is related to the exocyst, defining a family of complexes involved in multiple steps of membrane traffic
    J R Whyte
    MRC Laboratory of Molecular Biology, Cambridge, United Kingdom
    Dev Cell 1:527-37. 2001
    ....
  43. pmc Inositol phosphorylceramide synthase is located in the Golgi apparatus of Saccharomyces cerevisiae
    T P Levine
    Medical Research Council Laboratory of Molecular Biology, Cambridge CB2 2QH, United Kingdom
    Mol Biol Cell 11:2267-81. 2000
    ....
  44. pmc The Arf family G protein Arl1 is required for secretory granule biogenesis in Drosophila
    Isabel L Torres
    MRC Laboratory of Molecular Biology, Francis Crick Avenue, Cambridge CB2 0QH, UK
    J Cell Sci 127:2151-60. 2014
    ..Arl1 does not appear to be required for AP-1 recruitment in all tissues, suggesting that it is crucially required to enhance Arf1 activation at the trans-Golgi in particular tissues. ..
  45. pmc Untangling the evolution of Rab G proteins: implications of a comprehensive genomic analysis
    Tobias H Klöpper
    MRC Laboratory of Molecular Biology, Hills Road, Cambridge CB2 0QH, UK
    BMC Biol 10:71. 2012
    ..Each organelle or vesicle class is typically associated with one or more Rab, with the Rabs present in a particular cell reflecting that cell's complement of organelles and trafficking routes...
  46. pmc Activity of the yeast MNN1 alpha-1,3-mannosyltransferase requires a motif conserved in many other families of glycosyltransferases
    C A Wiggins
    Medical Research Council Laboratory of Molecular Biology, Hills Road, Cambridge CB2 2QH, United Kingdom
    Proc Natl Acad Sci U S A 95:7945-50. 1998
    ....
  47. ncbi request reprint The Saccharomyces cerevisiae protein Mnn10p/Bed1p is a subunit of a Golgi mannosyltransferase complex
    J Jungmann
    Medical Research Council Laboratory of Molecular Biology, Hills Road, Cambridge, CB2 2QH, United Kingdom
    J Biol Chem 274:6579-85. 1999
    ....
  48. pmc Nomenclature for the human Arf family of GTP-binding proteins: ARF, ARL, and SAR proteins
    Richard A Kahn
    Department of Biochemistry, Emory University School of Medicine, Atlanta, GA 30322, USA
    J Cell Biol 172:645-50. 2006
    ..We propose a more consistent nomenclature for the human members of the Arf family that may also serve as a guide for nomenclature in other species...
  49. ncbi request reprint Cell biology: earthworms and lipid couriers
    Sean Munro
    Nature 426:775-6. 2003
  50. ncbi request reprint Targeting of Golgi-specific pleckstrin homology domains involves both PtdIns 4-kinase-dependent and -independent components
    Timothy P Levine
    Division of Cell Biology, Institute of Ophthalmology, University College London, Bath Street, London EC1V 9EL, UK
    Curr Biol 12:695-704. 2002
    ....
  51. ncbi request reprint More than one way to replicate the Golgi apparatus
    Sean Munro
    Nat Cell Biol 4:E223-4. 2002