Research Topics
Genomes and GenesSpecies | S J MorleySummaryAffiliation: University of Sussex Country: UK Publications
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Publications
Involvement of stress-activated protein kinase and p38/RK mitogen-activated protein kinase signaling pathways in the enhanced phosphorylation of initiation factor 4E in NIH 3T3 cellsS J Morley
Biochemistry Laboratory, School of Biological Sciences, University of Sussex, Falmer, Brighton BN1 9QG, United Kingdom
J Biol Chem 272:17887-93. 1997....
Kinky binding and SECsy insertionsSimon J Morley
Department of Biochemistry, School of Life Sciences, University of Sussex, Brighton BN1 9QG, UK
Mol Cell 35:396-8. 2009....- Signaling pathways open the GAITway to translational controlSimon J Morley
Department of Biochemistry, School of Life Sciences, University of Sussex, Falmer, Brighton BN1 9QG, UK
Dev Cell 15:639-40. 2008..in a recent issue of Molecular Cell, show that the kinases themselves are regulated by the same translational silencing they promote thereby providing a negative feedback loop to limit late inflammatory gene expression... - A cunning stunt: an alternative mechanism of eukaryotic translation initiationSimon J Morley
Department of Biochemistry, School of Life Sciences, University of Sussex, Brighton BN1 9QG, UK
Sci Signal 1:pe32. 2008.... - Initiation factor modifications in the preapoptotic phaseS J Morley
Department of Biochemistry, School of Life Sciences, University of Sussex, Falmer, Brighton BN1 9QG, UK
Cell Death Differ 12:571-84. 2005.... - Phosphorylation of initiation factor 4E is resistant to SB203580 in cells expressing a drug-resistant mutant of stress-activated protein kinase 2a/p38Simon J Morley
Biochemistry Laboratory, School of Biological Sciences, University of Sussex, Falmer, BN1 9QG, Brighton, UK
Cell Signal 15:741-9. 2003..Therefore, this study validates the use of SB203580 for investigating signalling pathways modulating the phosphorylation of eIF4E in cultured cells... - Phosphorylation of eukaryotic initiation factor (eIF) 4E is not required for de novo protein synthesis following recovery from hypertonic stress in human kidney cellsSimon J Morley
Biochemistry Laboratory, School of Biological Sciences, University of Sussex, Falmer, Brighton BN1 9QG, United Kingdom
J Biol Chem 277:32855-9. 2002..However, by using a cell-permeable, specific inhibitor of eIF4E kinase, Mnk1 (CGP57380), we show that de novo initiation of translation and eIF4F complex assembly during this recovery phase did not require eIF4E phosphorylation... - Proteasome inhibitors and immunosuppressive drugs promote the cleavage of eIF4GI and eIF4GII by caspase-8-independent mechanisms in Jurkat T cell linesS J Morley
Biochemistry Laboratory, School of Biological Sciences, University of Sussex, Falmer, Brighton, BN1 9QG, UK
FEBS Lett 503:206-12. 2001..These data suggest that caspase-8 activation is not required for apoptosis and eIF4G cleavage mediated by proteasome inhibitors and immunosuppressants in human T cells... - Signalling through either the p38 or ERK mitogen-activated protein (MAP) kinase pathway is obligatory for phorbol ester and T cell receptor complex (TCR-CD3)-stimulated phosphorylation of initiation factor (eIF) 4E in Jurkat T cellsS J Morley
Biochemistry Laboratory, School of Biological Sciences, University of Sussex, Brighton, UK
FEBS Lett 418:327-32. 1997..These data show that in Jurkat cells, protein kinase C modulates the phosphorylation status of eIF4E indirectly via the ERK and/or p38 MAP kinase signalling pathways... - Cleavage of translation initiation factor 4G (eIF4G) during anti-Fas IgM-induced apoptosis does not require signalling through the p38 mitogen-activated protein (MAP) kinaseS J Morley
Biochemistry Laboratory, School of Biological Sciences, University of Sussex, Falmer, Brighton, UK
FEBS Lett 438:41-8. 1998..These data suggest that the cleavage of eIF4G and the inhibition of translation play an integral role in Fas/CD95-induced cell death in Jurkat cells... 
Activity of the hepatitis A virus IRES requires association between the cap-binding translation initiation factor (eIF4E) and eIF4GI K Ali
Department of Biochemistry, University of Cambridge, Cambridge CB2 1GA, United Kingdom
J Virol 75:7854-63. 2001....- Differential requirements for caspase-8 activity in the mechanism of phosphorylation of eIF2alpha, cleavage of eIF4GI and signaling events associated with the inhibition of protein synthesis in apoptotic Jurkat T cellsS J Morley
Biochemistry Laboratory, School of Biological Sciences, University of Sussex, Falmer, Brighton BN1 9QG, UK
FEBS Lett 477:229-36. 2000..These data suggest that apoptosis impinges upon the activity of several polypeptides which are central to the regulation of protein synthesis and that multiple signaling pathways are involved in vivo... - Changes in integrity and association of eukaryotic protein synthesis initiation factors during apoptosisM Bushell
Biochemistry Group, School of Biological Sciences, University of Sussex, Brighton, UK
Eur J Biochem 267:1083-91. 2000..These events are likely to contribute to the inhibition of protein synthesis seen under these conditions... - Truncated initiation factor eIF4G lacking an eIF4E binding site can support capped mRNA translationI K Ali
Department of Biochemistry, University of Cambridge, 80 Tennis Court Road, Cambridge CB2 1GA, UK
EMBO J 20:4233-42. 2001..Our results imply that picornavirus-induced shut-off is not due to an intrinsic inability of p100 to support capped mRNA translation, but to the viral RNA outcompeting host cell mRNA for the limiting concentration of p100... - Phosphorylation of eukaryotic initiation factor 4E (eIF4E) at Ser209 is not required for protein synthesis in vitro and in vivoL McKendrick
School of Biological Sciences, University of Sussex, Brighton, UK
Eur J Biochem 268:5375-85. 2001..Furthermore, we show that wild-type and phosphorylation-site variants of eIF4E protein are equally able to rescue the lethal phenotype of eIF4E deletion in S. cerevisiae... - The proteasome inhibitor, MG132, promotes the reprogramming of translation in C2C12 myoblasts and facilitates the association of hsp25 with the eIF4F complexJoanne L Cowan
Department of Biochemistry, School of Life Sciences, University of Sussex, Falmer, Brighton, UK
Eur J Biochem 271:3596-611. 2004.... - Utilizing the peptidyl-prolyl cis-trans isomerase pin1 as a probe of its phosphorylated target proteins. Examples of binding to nuclear proteins in a human kidney cell line and to tau in Alzheimer's diseased brainJ R Thorpe
Electron Microscope and FACS Laboratory, School of Biological Sciences, University of Sussex, Brighton, United Kingdom
J Histochem Cytochem 49:97-108. 2001.... - Translation initiation factor 4EL McKendrick
Biochemistry Laboratory, School of Biological Sciences, University of Sussex, Falmer, Brighton, UK
Int J Biochem Cell Biol 31:31-5. 1999..The function of this review is to summarise what is known about eIF4E gene and protein structure, biological function and medical relevance... - Interaction of eukaryotic translation initiation factor 4G with the nuclear cap-binding complex provides a link between nuclear and cytoplasmic functions of the m(7) guanosine capL McKendrick
Department of Biochemistry, School of Biological Sciences, University of Sussex, Falmer, Brighton BN1 9QG, United Kingdom
Mol Cell Biol 21:3632-41. 2001..This may provide a mechanism to couple nuclear and cytoplasmic functions of the mRNA cap structure... - Shortfalls in the peptidyl-prolyl cis-trans isomerase protein Pin1 in neurons are associated with frontotemporal dementiasJulian R Thorpe
Electron Microscope Division, The Sussex Centre for Advanced Microscopy, School of Life Sciences, University of Sussex, Brighton, UK
Neurobiol Dis 17:237-49. 2004..This may be a unifying, contributory factor towards neuronal death in these dementias... - Phosphorylation by aurora-B negatively regulates survivin function during mitosisSally P Wheatley
Genome Damage and Stability Centre, University of Sussex, Falmer, Brighton, UK
Cell Cycle 6:1220-30. 2007..We conclude that survivin is phosphorylated at T117 during mitosis, and once phosphorylated, dephosphorylation is crucial for chromosome congression and progression into anaphase... - Molecular cross-talk between MEK1/2 and mTOR signaling during recovery of 293 cells from hypertonic stressSusanne Naegele
Biochemistry Laboratory, School of Life Sciences, University of Sussex, Falmer, Brighton BN1 9QG, United Kingdom
J Biol Chem 279:46023-34. 2004.... - Expression of fragments of translation initiation factor eIF4GI reveals a nuclear localisation signal within the N-terminal apoptotic cleavage fragment N-FAGMark J Coldwell
School of Life Sciences, University of Sussex, Falmer, Brighton, BN1 9QG, UK
J Cell Sci 117:2545-55. 2004..In addition, the presence or absence of the sequence flanking and including the eIF4E binding site (residues 533-682) confers a distinct cellular distribution pattern for the central domain of eIF4GI... - Specific isoforms of translation initiation factor 4GI show differences in translational activityMark J Coldwell
Department of Biochemistry, School of Life Sciences, University of Sussex, Falmer, Brighton BN1 9QG, United Kingdom
Mol Cell Biol 26:8448-60. 2006.... - Functional analysis of individual binding activities of the scaffold protein eIF4GTracey M Hinton
Department of Biochemistry, School of Life Sciences, University of Sussex, Falmer, Brighton BN1 9QG, United Kingdom
J Biol Chem 282:1695-708. 2007..We conclude that there is considerable redundancy in the mechanisms forming initiation complexes in mammalian cells, such that many individual interactions have regulatory rather than essential roles... - Overproduction of a conserved domain of fission yeast and mammalian translation initiation factor eIF4G causes aberrant cell morphology and results in disruption of the localization of F-actin and the organization of microtubulesLida Hashemzadeh-Bonehi
Biochemistry Laboratory, School of Biological Sciences, University of Sussex, Brighton BN1 9QG, UK
Genes Cells 8:163-78. 2003..Here we report specific effects of the over-expression of human and fission yeast eIF4G domains on cell morphology in Schizosaccharomyces pombe... - Localization of ribosomes and translation initiation factors to talin/beta3-integrin-enriched adhesion complexes in spreading and migrating mammalian cellsMark Willett
Department of Biochemistry, School of Life Sciences, University of Sussex, Brighton BN1 9QG, U K
Biol Cell 102:265-76. 2010..The spatial localization of translation can facilitate the enrichment of proteins at their sites of function while also ensuring that proteins are expressed in the proximity of their cognate binding partners... - Paxillin associates with poly(A)-binding protein 1 at the dense endoplasmic reticulum and the leading edge of migrating cellsAlison J Woods
Department of Biochemistry, University of Leicester, University Road, Leicester, LE1 7RH, United Kingdom
J Biol Chem 277:6428-37. 2002.... - Compartmentalisation and localisation of the translation initiation factor (eIF) 4F complex in normally growing fibroblastsMark Willett
Department of Biochemistry, School of Life Sciences, University of Sussex, Falmer, Brighton BN1 9QG, UK
Exp Cell Res 312:2942-53. 2006.... - Inhibition of mammalian target of rapamycin (mTOR) signalling in C2C12 myoblasts prevents myogenic differentiation without affecting the hyperphosphorylation of 4E-BP1Mark Willett
Department of Biochemistry, School of Life Sciences, University of Sussex, Falmer, Brighton BN19QG, UK
Cell Signal 21:1504-12. 2009..In contrast, eIF4E appears to be under translational control with a significant delay between induction of mRNA and subsequent protein expression... - In vitro cleavage of eIF4GI but not eIF4GII by HIV-1 protease and its effects on translation in the rabbit reticulocyte lysate systemTheophile Ohlmann
LaboRetro, INSERM U412, Ecole Normale Superieure de Lyon, 46 allee d Italie, 69364 Lyon Cedex 07, France
J Mol Biol 318:9-20. 2002.... - The histone 3'-terminal stem-loop-binding protein enhances translation through a functional and physical interaction with eukaryotic initiation factor 4G (eIF4G) and eIF3Jun Ling
Department of Biochemistry, University of California, Riverside, California 92521-0129, USA
Mol Cell Biol 22:7853-67. 2002..These data indicate that SLBP stimulates the translation of histone mRNAs through a functional interaction with both the mRNA stem-loop and the 5' cap that is mediated by eIF4G and eIF3... - Inhibition of cap-dependent translation via phosphorylation of eIF4G by protein kinase Pak2Jun Ling
Department of Biochemistry, University of California, Riverside, CA 92521, USA
EMBO J 24:4094-105. 2005..Thus, a novel pathway for mammalian cell stress signaling is identified, wherein activation of Pak2 leads to inhibition of cap-dependent translation through phosphorylation of eIF4G... - Nutrient-responsive mTOR signalling grows on Sterile groundSimon J Cook
Laboratory of Molecular Signalling, The Babraham Institute, Babraham Research Campus, Cambridge, CB22 3AT, UK
Biochem J 403:e1-3. 2007..In this context, MAP4K3 looks like an attractive drug target since inhibitors of this enzyme should switch off mTOR, thereby inhibiting cell growth and proliferation, and promoting apoptosis... - Increased levels of the translation initiation factor eIF4E in differentiating epithelial lung tumor cell linesDerek Walsh
National Cell and Tissue Culture Centre/National Institute for Cellular Biotechnology, Dublin City University, Glasnevin, Ireland
Differentiation 71:126-34. 2003.... - Rapamycin-sensitive induction of eukaryotic initiation factor 4F in regenerating mouse liverMelissa M Goggin
Division of Gastroenterology, Hennepin County Medical Center, Minneapolis, MN 55415, USA
Hepatology 40:537-44. 2004..Further study of factors that regulate translation initiation may provide insight into mechanisms that govern metabolic homeostasis and regeneration in response to liver injury...