Hywel Morgan

Summary

Affiliation: University of Southampton
Country: UK

Publications

  1. ncbi request reprint A sheath-less combined optical and impedance micro-cytometer
    Daniel Spencer
    Faculty of Physical Sciences and Engineering, and Institute for Life Sciences, University of Southampton, Southampton, Hampshire SO17 1BJ, UK
    Lab Chip 14:3064-73. 2014
  2. ncbi request reprint StyletChip: a microfluidic device for recording host invasion behaviour and feeding of plant parasitic nematodes
    Chunxiao Hu
    Faculty of Physical Sciences and Engineering, and Institute for Life Sciences, University of Southampton, Southampton, SO17 1BJ, UK
    Lab Chip 14:2447-55. 2014
  3. ncbi request reprint Droplet-based in situ compartmentalization of chemically separated components after isoelectric focusing in a Slipchip
    Yan Zhao
    Faculty of Physical Sciences and Engineering, and Institute for Life Sciences, University of Southampton, Highfield, Southampton, SO17 1BJ, UK
    Lab Chip 14:555-61. 2014
  4. ncbi request reprint Conference Scene: NanoBiotech Montreux 2009
    Veronica Hollis
    School of Electronics and Computer Science, University of Southampton, University Road, Southampton SO17 1BJ, UK
    Nanomedicine (Lond) 5:177-9. 2010
  5. ncbi request reprint Positional dependence of particles in microfludic impedance cytometry
    Daniel Spencer
    School of Electronics and Computing Science, University of Southampton, Highfield, Southampton SO17 1BJ, UK
    Lab Chip 11:1234-9. 2011
  6. ncbi request reprint Simultaneous high speed optical and impedance analysis of single particles with a microfluidic cytometer
    David Barat
    School of Electronics and Computer Science, University of Southampton, Highfield, Southampton, SO17 1BJ, UK
    Lab Chip 12:118-26. 2012
  7. ncbi request reprint Impedance spectroscopy using maximum length sequences: application to single cell analysis
    Shady Gawad
    Nanoscale Systems Integration Group, School of Electronics and Computer Science, University of Southampton, United Kingdom
    Rev Sci Instrum 78:054301. 2007
  8. ncbi request reprint High throughput particle analysis: combining dielectrophoretic particle focussing with confocal optical detection
    David Holmes
    School of Electronics and Computing Science, University of Southampton, Highfield, UK
    Biosens Bioelectron 21:1621-30. 2006
  9. doi request reprint Micro-impedance cytometry for detection and analysis of micron-sized particles and bacteria
    Catia Bernabini
    School of Electronics and Computer Science, University of Southampton, Highfield, Southampton, SO17 1BJ, UK
    Lab Chip 11:407-12. 2011
  10. doi request reprint Electrophysiological characterization of membrane disruption by nanoparticles
    Maurits R R de Planque
    School of Electronics and Computer Science, University of Southampton, Southampton SO17 1BJ, United Kingdom
    ACS Nano 5:3599-606. 2011

Collaborators

  • David Holmes
  • Nicolas G Green
  • Michele Zagnoni
  • Mairi E Sandison
  • Tao Sun
  • James Kearn
  • Lindy Holden-Dye
  • Shady Gawad
  • D E Davies
  • Yan Zhao
  • Sam Birtwell
  • A G Lee
  • Daniel Spencer
  • Chunxiao Hu
  • Sara Aghdaei
  • Graham R Broder
  • Gabriel Cavalli
  • David Barat
  • Maurits R R de Planque
  • Catia Bernabini
  • Cedric F A Floquet
  • Veronica Hollis
  • Rupert S Thomas
  • Phedra Marius
  • Peter L Roach
  • Shahanara Banu
  • Hugo F P Martins
  • Cameron Neylon
  • Rohan T Ranasinghe
  • Irina Ermolina
  • Vincent O' Connor
  • Peter Urwin
  • Catherine Lilley
  • Gregor Elliott
  • James Dillon
  • Caitriona Murray
  • Vincent O'Connor
  • Matthew Charles Mowlem
  • Giuseppe Benazzi
  • Matthew C Mowlem
  • Ambra Milani
  • Etienne P Joly
  • Vincent J Sieben
  • Iain R G Ogilvie
  • Tiina Roose
  • J Malcolm East
  • Nikolay Zheludev
  • Gerasim S Galitonov
  • Joseph K She
  • Kevin F Macdonald
  • Mark Bradley
  • Joel Milner

Detail Information

Publications29

  1. ncbi request reprint A sheath-less combined optical and impedance micro-cytometer
    Daniel Spencer
    Faculty of Physical Sciences and Engineering, and Institute for Life Sciences, University of Southampton, Southampton, Hampshire SO17 1BJ, UK
    Lab Chip 14:3064-73. 2014
    ..The integration of impedance, fluorescence and side scatter into a single miniature cytometer platform provides the core information content of a classical cytometer in a highly compact, simple, portable and low cost format. ..
  2. ncbi request reprint StyletChip: a microfluidic device for recording host invasion behaviour and feeding of plant parasitic nematodes
    Chunxiao Hu
    Faculty of Physical Sciences and Engineering, and Institute for Life Sciences, University of Southampton, Southampton, SO17 1BJ, UK
    Lab Chip 14:2447-55. 2014
    ..The unique approach is optimised for trapping and recording from smaller worms or worms with distinct anterior body shapes and may be applied to other species of economic or medical importance. ..
  3. ncbi request reprint Droplet-based in situ compartmentalization of chemically separated components after isoelectric focusing in a Slipchip
    Yan Zhao
    Faculty of Physical Sciences and Engineering, and Institute for Life Sciences, University of Southampton, Highfield, Southampton, SO17 1BJ, UK
    Lab Chip 14:555-61. 2014
    ..Compared to alternative approaches for sample collection, the method avoids remixing, is scalable and is easily hyphenated with the other analytical methods. ..
  4. ncbi request reprint Conference Scene: NanoBiotech Montreux 2009
    Veronica Hollis
    School of Electronics and Computer Science, University of Southampton, University Road, Southampton SO17 1BJ, UK
    Nanomedicine (Lond) 5:177-9. 2010
    ..With over 130 delegates from more than 25 countries worldwide, NanoBioTech 2009 provided a diverse and invigorating forum for discussion...
  5. ncbi request reprint Positional dependence of particles in microfludic impedance cytometry
    Daniel Spencer
    School of Electronics and Computing Science, University of Southampton, Highfield, Southampton SO17 1BJ, UK
    Lab Chip 11:1234-9. 2011
    ..At high flow rates inertial focusing is observed; the particles flow in equal numbers through two equilibrium positions reducing the coefficient of variance (CV) in the impedance signals to negligible values...
  6. ncbi request reprint Simultaneous high speed optical and impedance analysis of single particles with a microfluidic cytometer
    David Barat
    School of Electronics and Computer Science, University of Southampton, Highfield, Southampton, SO17 1BJ, UK
    Lab Chip 12:118-26. 2012
    ..The system is characterised using a range of different sized polystyrene beads (fluorescent and non-fluorescent). Single and mixed populations of beads were measured and the data compared with a conventional flow cytometer...
  7. ncbi request reprint Impedance spectroscopy using maximum length sequences: application to single cell analysis
    Shady Gawad
    Nanoscale Systems Integration Group, School of Electronics and Computer Science, University of Southampton, United Kingdom
    Rev Sci Instrum 78:054301. 2007
    ..The technique is demonstrated using a time-invariant passive RC network. The impedance of single biological cell flowing in a microfluidic channel is also measured, showing that MLS is an ideal method for high speed impedance analysis...
  8. ncbi request reprint High throughput particle analysis: combining dielectrophoretic particle focussing with confocal optical detection
    David Holmes
    School of Electronics and Computing Science, University of Southampton, Highfield, UK
    Biosens Bioelectron 21:1621-30. 2006
    ....
  9. doi request reprint Micro-impedance cytometry for detection and analysis of micron-sized particles and bacteria
    Catia Bernabini
    School of Electronics and Computer Science, University of Southampton, Highfield, Southampton, SO17 1BJ, UK
    Lab Chip 11:407-12. 2011
    ..Data are also compared with conventional flow cytometry and dynamic light scattering: the coefficient of variation (CV) of size is found to be comparable between the systems...
  10. doi request reprint Electrophysiological characterization of membrane disruption by nanoparticles
    Maurits R R de Planque
    School of Electronics and Computer Science, University of Southampton, Southampton SO17 1BJ, United Kingdom
    ACS Nano 5:3599-606. 2011
    ..This biophysical approach gives quantitative insight into nanosphere-bilayer interactions and suggests that nanoparticle-lipid interactions alone can compromise the barrier function of the plasma membrane...
  11. doi request reprint Nanomolar detection with high sensitivity microfluidic absorption cells manufactured in tinted PMMA for chemical analysis
    Cedric F A Floquet
    National Oceanography Centre, University of Southampton, Southampton SO14 3ZH, UK
    Talanta 84:235-9. 2011
    ..The method is used to achieve a LOD of 20 nM with a colourimetric iron assay and a LOD of 0.22 milli-absorption units with a pH assay...
  12. ncbi request reprint On-chip epithelial barrier function assays using electrical impedance spectroscopy
    Tao Sun
    Nano Research Group, School of Electronics and Computer Science, University of Southampton, SO17 1BJ, United Kingdom
    Lab Chip 10:1611-7. 2010
    ..Immunofluorescent staining of the ZO-1 tight junction protein in the untreated and treated 16HBEs was performed to verify the disruption of the tight junctions by EGTA...
  13. doi request reprint Single cell impedance cytometry for identification and counting of CD4 T-cells in human blood using impedance labels
    David Holmes
    School of Electronics and Computing Science, University of Southampton, Highfield, Southampton, SO17 1BJ, UK
    Anal Chem 82:1455-61. 2010
    ..The technique represents a simple method for detecting a subpopulation of cells within a heterogeneous mix. The impedance-based antibody identification method could form the basis of simple low-cost point of care diagnostic technologies...
  14. doi request reprint Microparticle encoding technologies for high-throughput multiplexed suspension assays
    Sam Birtwell
    School of Electronics and Computer Science, University of Southampton, Southampton, UK
    Integr Biol (Camb) 1:345-62. 2009
    ..As a result, we identify the most likely candidates to be used for future integrated device development for practical applications...
  15. ncbi request reprint On-chip electrical impedance tomography for imaging biological cells
    Tao Sun
    School of Electronics and Computer Science, University of Southampton, United Kingdom
    Biosens Bioelectron 25:1109-15. 2010
    ..Our system provides a non-invasive lab-on-a-chip technology for spatially mapping the electrical properties of single cells, which would be significant and useful for diagnostic and clinical applications...
  16. doi request reprint Leukocyte analysis and differentiation using high speed microfluidic single cell impedance cytometry
    David Holmes
    School of Electronics and Computing Science, University of Southampton, Highfield, Southampton, SO17 1BJ, UK
    Lab Chip 9:2881-9. 2009
    ..Tests with patient samples showed 95% correlation against commercial (optical/Coulter) blood analysis equipment, demonstrating the potential clinical utility of the impedance microcytometer for a point-of-care blood analysis system...
  17. ncbi request reprint Air-exposure technique for the formation of artificial lipid bilayers in microsystems
    Mairi E Sandison
    School of Electronics and Computer Science, University of Southampton, Southampton, United Kingdom
    Langmuir 23:8277-84. 2007
    ..Scaling up the technique for use in an array platform is also straightforward, the simultaneous formation of three individually addressable BLMs being demonstrated...
  18. ncbi request reprint High speed multi-frequency impedance analysis of single particles in a microfluidic cytometer using maximum length sequences
    Tao Sun
    Nanoscale Systems Integration Group, School of Electronics and Computer Science, University of Southampton, United Kingdom
    Lab Chip 7:1034-40. 2007
    ..5625 Hz to 500 kHz. The spectral information for each bead is obtained in approximately 1 ms. Good agreement is shown between the MLS data and both circuit simulations and conventional AC single frequency measurements...
  19. ncbi request reprint Bead-based immunoassays using a micro-chip flow cytometer
    David Holmes
    School of Electronics and Computing Science, University of Southampton, Highfield, Southampton, UK SO17 1BJ
    Lab Chip 7:1048-56. 2007
    ..The data from the microfabricated flow cytometer was shown to be comparable to that of a commercial flow cytometer (BD-FACSAria)...
  20. pmc Controlled delivery of proteins into bilayer lipid membranes on chip
    Michele Zagnoni
    NSI Group, School of Electronics and Computer Science, University of Southampton, Highfield, Southampton, UK
    Lab Chip 7:1176-83. 2007
    ..Single channel conductance measurements of KcsA in the on-chip bilayer were recorded and these were compared to previously published data obtained with a conventional planar bilayer system...
  21. pmc NeuroChip: a microfluidic electrophysiological device for genetic and chemical biology screening of Caenorhabditis elegans adult and larvae
    Chunxiao Hu
    Hybrid Biodevices Group, Electronics and Computer Science, University of Southampton, Southampton, United Kingdom
    PLoS ONE 8:e64297. 2013
    ..NeuroChip thus adds a new tool for exploitation of C. elegans and has applications in neurogenetics, drug discovery and neurotoxicology...
  22. doi request reprint Negative DEP traps for single cell immobilisation
    Rupert S Thomas
    The Nano Group, University of Southampton, Southampton, UK
    Lab Chip 9:1534-40. 2009
    ..Analysis of the 80 microm diameter trap showed that a 15.6 microm diameter latex particle could be held with a force of 23 pN at an applied voltage of 5 V peak-peak...
  23. ncbi request reprint Microdevices for dielectrophoretic flow-through cell separation
    David Holmes
    School of Electronics and Computer Science, University of Southhampton, Highfield, Southhampton SO17 1BJ, UK
    IEEE Eng Med Biol Mag 22:85-90. 2003
  24. doi request reprint Diffractive micro bar codes for encoding of biomolecules in multiplexed assays
    Graham R Broder
    School of Chemistry, School of Electronics and Computer Science, Optoelectronics Research Centre, and School of Physics and Astronomy, University of Southampton, Highfield, Southampton, SO17 1BJ, UK
    Anal Chem 80:1902-9. 2008
    ....
  25. ncbi request reprint Bilayer lipid membranes from falling droplets
    Michele Zagnoni
    School of Electronics and Computer Science, University of Southampton, Highfield, SO17 1BJ, Southampton, UK
    Anal Bioanal Chem 393:1601-5. 2009
    ..This method is suitable for incorporation into lab-on-a-chip devices and allows for buffer exchange and electrical measurements...
  26. ncbi request reprint Multistep synthesis on SU-8: combining microfabrication and solid-phase chemistry on a single material
    Gabriel Cavalli
    School of Chemistry, Highfield Campus, University of Southampton, Southampton SO17 1BJ, UK
    J Comb Chem 9:462-72. 2007
    ....
  27. pmc Binding of anionic lipids to at least three nonannular sites on the potassium channel KcsA is required for channel opening
    Phedra Marius
    School of Biological Sciences, University of Southampton, Southampton, United Kingdom
    Biophys J 94:1689-98. 2008
    ..The conductance of the open channel increases with increasing concentration of anionic lipid, an effect possibly due to effects of anionic lipid on the concentration of K(+) close to the membrane surface...
  28. doi request reprint Formation of artificial lipid bilayers using droplet dielectrophoresis
    Sara Aghdaei
    School of Electronics and Computer Science, University of Southampton, Southampton, UK
    Lab Chip 8:1617-20. 2008
    ..The method produced BLMs suitable for single-channel recording of membrane protein activity and the technique can be extended to create programmable BLM arrays and networks...
  29. ncbi request reprint Dielectrophoretic investigation of plant virus particles: Cow Pea Mosaic Virus and Tobacco Mosaic Virus
    Irina Ermolina
    School of Electronics and Computer Science, University of Southampton, Southampton, UK
    Electrophoresis 27:3939-48. 2006
    ..3 nS for CPMV, 0.38 nS for TMV, and 0.52 nS for 27 nm diameter carboxy-latex beads. Data analysis has shown that the optimal condition for separation of TMV and CPMV is a low-conductivity suspending medium - below 1 mS/m...