Anthony J Morgan

Summary

Affiliation: University of Oxford
Country: UK

Publications

  1. ncbi request reprint NAADP: a new second messenger for glucose-induced Ca2+ responses in clonal pancreatic beta cells
    Roser Masgrau
    Department of Pharmacology, University of Oxford, Mansfield Road, Oxford OX1 3QT, United Kingdom
    Curr Biol 13:247-51. 2003
  2. pmc The luminal Ca(2+) chelator, TPEN, inhibits NAADP-induced Ca(2+) release
    Anthony J Morgan
    Department of Pharmacology, University of Oxford, Oxford, UK
    Cell Calcium 52:481-7. 2012
  3. doi request reprint Sea urchin eggs in the acid reign
    Anthony J Morgan
    Department of Pharmacology, University of Oxford, Mansfield Road, Oxford, OX1 3QT, United Kingdom
    Cell Calcium 50:147-56. 2011
  4. doi request reprint Molecular mechanisms of endolysosomal Ca2+ signalling in health and disease
    Anthony J Morgan
    Department of Pharmacology, University of Oxford, U K
    Biochem J 439:349-74. 2011
  5. pmc NAADP induces pH changes in the lumen of acidic Ca2+ stores
    Anthony J Morgan
    Department of Pharmacology, University of Oxford, Mansfield Road, Oxford OX1 3QT, UK
    Biochem J 402:301-10. 2007
  6. ncbi request reprint Fertilization and nicotinic acid adenine dinucleotide phosphate induce pH changes in acidic Ca(2+) stores in sea urchin eggs
    Anthony J Morgan
    Department of Pharmacology, University of Oxford, Oxford, UK
    J Biol Chem 282:37730-7. 2007
  7. doi request reprint Investigating cADPR and NAADP in intact and broken cell preparations
    Anthony J Morgan
    Department of Pharmacology, University of Oxford, Mansfield Road, Oxford, Oxon OX1 3QT, UK
    Methods 46:194-203. 2008
  8. pmc Purified TPC isoforms form NAADP receptors with distinct roles for Ca(2+) signaling and endolysosomal trafficking
    Margarida Ruas
    Department of Pharmacology, University of Oxford, Oxford OX1 3QT, UK
    Curr Biol 20:703-9. 2010
  9. pmc Bidirectional Ca²⁺ signaling occurs between the endoplasmic reticulum and acidic organelles
    Anthony J Morgan
    Department of Pharmacology, University of Oxford, Oxford OX1 3QT, England, UK
    J Cell Biol 200:789-805. 2013
  10. doi request reprint NAADP as an intracellular messenger regulating lysosomal calcium-release channels
    Antony Galione
    Department of Pharmacology, University of Oxford, Mansfield Road, Oxford OX1 3QT, UK
    Biochem Soc Trans 38:1424-31. 2010

Collaborators

Detail Information

Publications27

  1. ncbi request reprint NAADP: a new second messenger for glucose-induced Ca2+ responses in clonal pancreatic beta cells
    Roser Masgrau
    Department of Pharmacology, University of Oxford, Mansfield Road, Oxford OX1 3QT, United Kingdom
    Curr Biol 13:247-51. 2003
    ....
  2. pmc The luminal Ca(2+) chelator, TPEN, inhibits NAADP-induced Ca(2+) release
    Anthony J Morgan
    Department of Pharmacology, University of Oxford, Oxford, UK
    Cell Calcium 52:481-7. 2012
    ..We propose that, in the sea urchin egg, luminal Ca(2+) is important for gating native NAADP-regulated two-pore channels...
  3. doi request reprint Sea urchin eggs in the acid reign
    Anthony J Morgan
    Department of Pharmacology, University of Oxford, Mansfield Road, Oxford, OX1 3QT, United Kingdom
    Cell Calcium 50:147-56. 2011
    ..This review attempts to summarize what we currently know about egg acidic vesicles in the context of Ca(2+) signalling. The dynamics of Ca(2+) storage, Ca(2+) mobilization, proton fluxes and two-pore channels will be discussed...
  4. doi request reprint Molecular mechanisms of endolysosomal Ca2+ signalling in health and disease
    Anthony J Morgan
    Department of Pharmacology, University of Oxford, U K
    Biochem J 439:349-74. 2011
    ..These developments underscore a new central role for the endolysosomal system in cellular Ca2+ regulation and signalling...
  5. pmc NAADP induces pH changes in the lumen of acidic Ca2+ stores
    Anthony J Morgan
    Department of Pharmacology, University of Oxford, Mansfield Road, Oxford OX1 3QT, UK
    Biochem J 402:301-10. 2007
    ..The results of the present study further support acidic stores as targets for NAADP and for the first time reveal an adjunct role for NAADP in regulating the pH(L) of intracellular organelles...
  6. ncbi request reprint Fertilization and nicotinic acid adenine dinucleotide phosphate induce pH changes in acidic Ca(2+) stores in sea urchin eggs
    Anthony J Morgan
    Department of Pharmacology, University of Oxford, Oxford, UK
    J Biol Chem 282:37730-7. 2007
    ..Our data show that NAADP and physiological stimuli alter the pH within intracellular organelles and suggest that NAADP signals through pH as well as Ca(2+)...
  7. doi request reprint Investigating cADPR and NAADP in intact and broken cell preparations
    Anthony J Morgan
    Department of Pharmacology, University of Oxford, Mansfield Road, Oxford, Oxon OX1 3QT, UK
    Methods 46:194-203. 2008
    ....
  8. pmc Purified TPC isoforms form NAADP receptors with distinct roles for Ca(2+) signaling and endolysosomal trafficking
    Margarida Ruas
    Department of Pharmacology, University of Oxford, Oxford OX1 3QT, UK
    Curr Biol 20:703-9. 2010
    ..We propose that NAADP regulates endolysosomal Ca(2+) storage and release via TPCs and coordinates endoplasmic reticulum Ca(2+) release in a role that impacts on Ca(2+) signaling in health and disease [6]...
  9. pmc Bidirectional Ca²⁺ signaling occurs between the endoplasmic reticulum and acidic organelles
    Anthony J Morgan
    Department of Pharmacology, University of Oxford, Oxford OX1 3QT, England, UK
    J Cell Biol 200:789-805. 2013
    ..Bidirectional organelle communication may have wider implications for endo-lysosomal function as well as the generation of Ca(2+) oscillations and waves...
  10. doi request reprint NAADP as an intracellular messenger regulating lysosomal calcium-release channels
    Antony Galione
    Department of Pharmacology, University of Oxford, Mansfield Road, Oxford OX1 3QT, UK
    Biochem Soc Trans 38:1424-31. 2010
    ....
  11. ncbi request reprint Organelle selection determines agonist-specific Ca2+ signals in pancreatic acinar and beta cells
    Michiko Yamasaki
    Department of Pharmacology, University of Oxford, Mansfield Road, Oxford OX1 3QT, UK
    J Biol Chem 279:7234-40. 2004
    ....
  12. pmc Ca(2+) signaling occurs via second messenger release from intraorganelle synthesis sites
    Lianne C Davis
    Department of Pharmacology, University of Oxford, Mansfield Road, Oxford OX1 3QT, United Kingdom
    Curr Biol 18:1612-8. 2008
    ..This represents a novel signaling mechanism whereby an extracellular stimulus increases the concentration of a second messenger by promoting messenger transport from intraorganelle synthesis sites to the cytosol...
  13. pmc Altered distribution and function of natural killer cells in murine and human Niemann-Pick disease type C1
    Anneliese O Speak
    Department of Pharmacology, University of Oxford, Mansfield Road, Oxford, United Kingdom
    Blood 123:51-60. 2014
    ..These findings highlight the effects of lysosomal storage on the peripheral immune system. ..
  14. pmc NAADP activates two-pore channels on T cell cytolytic granules to stimulate exocytosis and killing
    Lianne C Davis
    Department of Pharmacology, University of Oxford, Oxford, UK
    Curr Biol 22:2331-7. 2012
    ..This study highlights a selective role for NAADP in stimulating exocytosis crucial for immune cell function and may impact on stimulus-secretion coupling in wider cellular contexts...
  15. doi request reprint Synthesis of [³²P]NAADP for the radioreceptor binding assay
    Antony Galione
    Department of Pharmacology, University of Oxford, Oxford OX1 3QT, United Kingdom
    Cold Spring Harb Protoc 2014:993-5. 2014
    ..Here, we describe the synthesis of [(32)P]NAADP for use in the radioreceptor binding assay. ..
  16. doi request reprint Synthesis of NAADP-AM as a membrane-permeant NAADP analog
    Antony Galione
    Department of Pharmacology, University of Oxford, Oxford OX1 3QT, United Kingdom
    Cold Spring Harb Protoc 2014:pdb.prot076927. 2014
    ..Instead, a cell-permeant acetoxymethyl ester derivative of NAADP (NAADP-AM) can be synthesized as described here and used to study NAADP-mediated Ca²⁺ release...
  17. doi request reprint Measurement of luminal pH of acidic stores as a readout for NAADP action
    Antony Galione
    Department of Pharmacology, University of Oxford, Oxford OX1 3QT, United Kingdom
    Cold Spring Harb Protoc 2014:pdb.prot076935. 2014
    ..When pHL increases, some AO is lost from the vesicle. As a result, the lower luminal AO concentration relieves the quenching and fluorescence increases in the lumen...
  18. doi request reprint Synthesis of caged NAADP
    Antony Galione
    Department of Pharmacology, University of Oxford, Oxford OX1 3QT, United Kingdom
    Cold Spring Harb Protoc 2014:pdb.prot076943. 2014
    ..In brief, a stable precursor of the caging reagent is made and converted to an unstable reactive reagent immediately before addition to the compound to be caged...
  19. doi request reprint Niemann-Pick disease type C1 is a sphingosine storage disease that causes deregulation of lysosomal calcium
    Emyr Lloyd-Evans
    Department of Pharmacology, University of Oxford, Mansfield Road, Oxford, UK
    Nat Med 14:1247-55. 2008
    ..This unique calcium phenotype represents a new target for therapeutic intervention, as elevation of cytosolic calcium with curcumin normalized NPC1 disease cellular phenotypes and prolonged survival of the NPC1 mouse...
  20. doi request reprint Preparation and use of sea urchin egg homogenates for studying NAADP-mediated Ca²⁺ release
    Antony Galione
    Department of Pharmacology, University of Oxford, Oxford OX1 3QT, United Kingdom
    Cold Spring Harb Protoc 2014:988-92. 2014
    ..Here we describe how to prepare sea urchin egg homogenate and use it to measure NAADP-mediated Ca(2+) release. ..
  21. doi request reprint Two-pore channels (TPCs): current controversies
    Anthony J Morgan
    Department of Pharmacology, University of Oxford, Oxford, UK
    Bioessays 36:173-83. 2014
    ..This paper aims critically to evaluate the evidence for TPCs as NAADP targets and to discuss how the new findings fit in with what we know about endo-lysosomal Ca(2+) stores. ..
  22. doi request reprint Imaging approaches to measuring lysosomal calcium
    Anthony J Morgan
    Department of Pharmacology, University of Oxford, Oxford, UK
    Methods Cell Biol 126:159-95. 2015
    ..This chapter discusses several complimentary approaches to monitor endolysosomal Ca(2+), with particular emphasis on the inherent pitfalls that can plague the unwary. ..
  23. pmc Ca2+ release from the endoplasmic reticulum of NY-ESO-1-specific T cells is modulated by the affinity of TCR and by the use of the CD8 coreceptor
    Ji Li Chen
    Weatherall Institute of Molecular Medicine, Wellcome Trust Centre for Human Genetics, Oxford, UK
    J Immunol 184:1829-39. 2010
    ..This, in turn, modulates Ca(2+) influx from the extracellular environment, ultimately controlling T cell activation...
  24. doi request reprint Preparation and use of sea urchin egg homogenates
    Anthony J Morgan
    Department of Pharmacology, University of Oxford, Oxford, UK
    Methods Mol Biol 1128:161-73. 2014
    ..For Ca(2+) (or pHL) measurements, homogenate is thawed and sequentially diluted in an intracellular-like medium and the fluorescence of Ca(2+)- or pHL-sensitive dyes monitored in a standard fluorimeter or plate-reader...
  25. doi request reprint 2'-deoxy cyclic adenosine 5'-diphosphate ribose derivatives: importance of the 2'-hydroxyl motif for the antagonistic activity of 8-substituted cADPR derivatives
    Bo Zhang
    Wolfson Laboratory of Medicinal Chemistry, Department of Pharmacy and Pharmacology, University of Bath, Claverton Down, Bath, UK
    J Med Chem 51:1623-36. 2008
    ..The 2'-OH group, without effect on the Ca (2+)-mobilizing ability of cADPR itself, is an important motif for the antagonistic activities of 8-substituted cADPR analogues...
  26. ncbi request reprint Single-cell and subcellular measurement of intracellular Ca2+ concentration
    Anthony J Morgan
    Physiology Group, Vascular Biology Research Centre, Biomedical Sciences Division, King s College London, UK
    Methods Mol Biol 312:87-117. 2006
  27. ncbi request reprint Early activation of the p42/p44MAPK pathway mediates adenosine-induced nitric oxide production in human endothelial cells: a novel calcium-insensitive mechanism
    Amanda W Wyatt
    Centre for Cardiovascular Biology and Medicine, GKT School of Biomedical Sciences, King s College London, Guy s Campus, London SE1 1UL, UK
    FASEB J 16:1584-94. 2002
    ....