Daniela Moralli

Summary

Affiliation: University of Oxford
Country: UK

Publications

  1. pmc HAC stability in murine cells is influenced by nuclear localization and chromatin organization
    Daniela Moralli
    Wellcome Trust Centre for Human Genetics, University of Oxford, Roosevelt Drive, Oxford, OX3 7BN, UK
    BMC Cell Biol 10:18. 2009
  2. doi request reprint Simultaneous visualization of FISH signals and bromo-deoxyuridine incorporation by formamide-free DNA denaturation
    Daniela Moralli
    Wellcome Trust Centre for Human Genetics, University of Oxford, Oxford, UK
    Methods Mol Biol 659:203-18. 2010
  3. pmc Functional human artificial chromosomes are generated and stably maintained in human embryonic stem cells
    Mohammad A Mandegar
    Wellcome Trust Centre for Human Genetics, University of Oxford, Roosevelt Drive, Oxford OX3 7BN, UK
    Hum Mol Genet 20:2905-13. 2011
  4. pmc Comparative study of artificial chromosome centromeres in human and murine cells
    Daniela Moralli
    Wellcome Trust Centre for Human Genetics, University of Oxford, Oxford, UK
    Eur J Hum Genet 21:948-56. 2013
  5. pmc Altered intra-nuclear organisation of heterochromatin and genes in ICF syndrome
    Andrew Jefferson
    Wellcome Trust Centre for Human Genetics, University of Oxford, Oxford, United Kingdom
    PLoS ONE 5:e11364. 2010
  6. pmc An improved technique for chromosomal analysis of human ES and iPS cells
    Daniela Moralli
    Wellcome Trust Centre for Human Genetics, University of Oxford, Roosevelt Drive, OX3 7BN, Oxford, UK
    Stem Cell Rev 7:471-7. 2011
  7. pmc A novel human artificial chromosome gene expression system using herpes simplex virus type 1 vectors
    Daniela Moralli
    Weatherall Institute of Molecular Medicine, University of Oxford, John Radcliffe Hospital, Headley Way, Oxford OX3 9DS, UK
    EMBO Rep 7:911-8. 2006
  8. pmc Simultaneous detection of FISH signals and bromo-deoxyuridine incorporation in fixed tissue cultured cells
    Daniela Moralli
    Wellcome Trust Centre for Human Genetics, University of Oxford, Oxford, United Kingdom
    PLoS ONE 4:e4483. 2009

Collaborators

Detail Information

Publications8

  1. pmc HAC stability in murine cells is influenced by nuclear localization and chromatin organization
    Daniela Moralli
    Wellcome Trust Centre for Human Genetics, University of Oxford, Roosevelt Drive, Oxford, OX3 7BN, UK
    BMC Cell Biol 10:18. 2009
    ..In some cell lines the HAC are lost over a short period of time, while in others the HAC become stable without acquiring murine DNA...
  2. doi request reprint Simultaneous visualization of FISH signals and bromo-deoxyuridine incorporation by formamide-free DNA denaturation
    Daniela Moralli
    Wellcome Trust Centre for Human Genetics, University of Oxford, Oxford, UK
    Methods Mol Biol 659:203-18. 2010
    ..Finally, the cell preparations are analysed with an epifluorescence microscope to determine if the sequence of interest incorporates BrdU and in which point of the S-phase...
  3. pmc Functional human artificial chromosomes are generated and stably maintained in human embryonic stem cells
    Mohammad A Mandegar
    Wellcome Trust Centre for Human Genetics, University of Oxford, Roosevelt Drive, Oxford OX3 7BN, UK
    Hum Mol Genet 20:2905-13. 2011
    ..This is the first report of successfully generating gene expressing de novo HAC in hESc, and is a significant step towards the genetic manipulation of stem cells and potential therapeutic applications...
  4. pmc Comparative study of artificial chromosome centromeres in human and murine cells
    Daniela Moralli
    Wellcome Trust Centre for Human Genetics, University of Oxford, Oxford, UK
    Eur J Hum Genet 21:948-56. 2013
    ..Our work highlights the importance and utilisation of HAC for understanding the epigenetic aspects of chromosome biology. ..
  5. pmc Altered intra-nuclear organisation of heterochromatin and genes in ICF syndrome
    Andrew Jefferson
    Wellcome Trust Centre for Human Genetics, University of Oxford, Oxford, United Kingdom
    PLoS ONE 5:e11364. 2010
    ....
  6. pmc An improved technique for chromosomal analysis of human ES and iPS cells
    Daniela Moralli
    Wellcome Trust Centre for Human Genetics, University of Oxford, Roosevelt Drive, OX3 7BN, Oxford, UK
    Stem Cell Rev 7:471-7. 2011
    ....
  7. pmc A novel human artificial chromosome gene expression system using herpes simplex virus type 1 vectors
    Daniela Moralli
    Weatherall Institute of Molecular Medicine, University of Oxford, John Radcliffe Hospital, Headley Way, Oxford OX3 9DS, UK
    EMBO Rep 7:911-8. 2006
    ..The work is a significant advance for HAC technology and the development of HAC gene expression systems in human cells...
  8. pmc Simultaneous detection of FISH signals and bromo-deoxyuridine incorporation in fixed tissue cultured cells
    Daniela Moralli
    Wellcome Trust Centre for Human Genetics, University of Oxford, Oxford, United Kingdom
    PLoS ONE 4:e4483. 2009
    ..Since the technique does not involve paraformaldehyde for cell fixation, or formamide for denaturation of the target DNA and in post-hybridisation washes, it represents a safer alternative to classical FISH techniques...