T M Mayhew

Summary

Affiliation: University of Nottingham
Country: UK

Publications

  1. request reprint
    Mayhew T, Bowles C, Yucel F. Hypobaric hypoxia and villous trophoblast: evidence that human pregnancy at high altitude (3600 m) perturbs epithelial turnover and coagulation-fibrinolysis in the intervillous space. Placenta. 2002;23:154-62 pubmed
    ..The findings are partly consistent with results from in vitro studies which indicate that hypoxia stimulates proliferation of cytotrophoblast but impairs fusion into syncytium. ..
  2. Mayhew T, Lucocq J. Developments in cell biology for quantitative immunoelectron microscopy based on thin sections: a review. Histochem Cell Biol. 2008;130:299-313 pubmed publisher
    ..Finally, we discuss labelling efficiency (the number of gold particles per target molecule) and describe how it can be estimated for volume- or surface-occupiers by combining stereological data with biochemical determinations. ..
  3. request reprint
    Mayhew T, Lucocq J, Griffiths G. Relative labelling index: a novel stereological approach to test for non-random immunogold labelling of organelles and membranes on transmission electron microscopy thin sections. J Microsc. 2002;205:153-64 pubmed
    ..Contingency table analysis allows labelling distributions in different groups of cells to be compared. Protocols are described and illustrated using worked specimen examples and real data...
  4. request reprint
    Mayhew T, Griffiths G, Lucocq J. Applications of an efficient method for comparing immunogold labelling patterns in the same sets of compartments in different groups of cells. Histochem Cell Biol. 2004;122:171-7 pubmed
    ..Its application is illustrated using datasets from immunolabelling studies designed to localise the KDEL receptor, phosphatidyl-inositol 4,5-bisphosphate, GLUT4 and rab4 at the electron microscopic level...
  5. Mayhew T, Lucocq J. Quantifying immunogold labelling patterns of cellular compartments when they comprise mixtures of membranes (surface-occupying) and organelles (volume-occupying). Histochem Cell Biol. 2008;129:367-78 pubmed publisher
    ..LDs of the chosen compartments are used to calculate RLI and chi-squared values and these serve to identify those compartments in which there is preferential labelling. The methods are illustrated by synthetic and real data...
  6. Mayhew T, Lucocq J. Multiple-labelling immunoEM using different sizes of colloidal gold: alternative approaches to test for differential distribution and colocalization in subcellular structures. Histochem Cell Biol. 2011;135:317-26 pubmed publisher
    ..These simple and efficient approaches should be of more immediate utility to those interested in codistribution and colocalization in multiple immunogold labelling investigations. ..
  7. Mayhew T. Mapping the distributions and quantifying the labelling intensities of cell compartments by immunoelectron microscopy: progress towards a coherent set of methods. J Anat. 2011;219:647-60 pubmed publisher
    ..Here, the current state of the art is reviewed and approaches are illustrated with virtual datasets...
  8. Mayhew T. Quantitative description of the spatial arrangement of organelles in a polarised secretory epithelial cell: the salivary gland acinar cell. J Anat. 1999;194 ( Pt 2):279-85 pubmed
    ..These findings provide quantitative confirmation of the polarised arrangement of zymogen granules within acinar cells and further support for the relative invariance of biological organisation between subjects...

Detail Information

Publications8

  1. request reprint
    Mayhew T, Bowles C, Yucel F. Hypobaric hypoxia and villous trophoblast: evidence that human pregnancy at high altitude (3600 m) perturbs epithelial turnover and coagulation-fibrinolysis in the intervillous space. Placenta. 2002;23:154-62 pubmed
    ..The findings are partly consistent with results from in vitro studies which indicate that hypoxia stimulates proliferation of cytotrophoblast but impairs fusion into syncytium. ..
  2. Mayhew T, Lucocq J. Developments in cell biology for quantitative immunoelectron microscopy based on thin sections: a review. Histochem Cell Biol. 2008;130:299-313 pubmed publisher
    ..Finally, we discuss labelling efficiency (the number of gold particles per target molecule) and describe how it can be estimated for volume- or surface-occupiers by combining stereological data with biochemical determinations. ..
  3. request reprint
    Mayhew T, Lucocq J, Griffiths G. Relative labelling index: a novel stereological approach to test for non-random immunogold labelling of organelles and membranes on transmission electron microscopy thin sections. J Microsc. 2002;205:153-64 pubmed
    ..Contingency table analysis allows labelling distributions in different groups of cells to be compared. Protocols are described and illustrated using worked specimen examples and real data...
  4. request reprint
    Mayhew T, Griffiths G, Lucocq J. Applications of an efficient method for comparing immunogold labelling patterns in the same sets of compartments in different groups of cells. Histochem Cell Biol. 2004;122:171-7 pubmed
    ..Its application is illustrated using datasets from immunolabelling studies designed to localise the KDEL receptor, phosphatidyl-inositol 4,5-bisphosphate, GLUT4 and rab4 at the electron microscopic level...
  5. Mayhew T, Lucocq J. Quantifying immunogold labelling patterns of cellular compartments when they comprise mixtures of membranes (surface-occupying) and organelles (volume-occupying). Histochem Cell Biol. 2008;129:367-78 pubmed publisher
    ..LDs of the chosen compartments are used to calculate RLI and chi-squared values and these serve to identify those compartments in which there is preferential labelling. The methods are illustrated by synthetic and real data...
  6. Mayhew T, Lucocq J. Multiple-labelling immunoEM using different sizes of colloidal gold: alternative approaches to test for differential distribution and colocalization in subcellular structures. Histochem Cell Biol. 2011;135:317-26 pubmed publisher
    ..These simple and efficient approaches should be of more immediate utility to those interested in codistribution and colocalization in multiple immunogold labelling investigations. ..
  7. Mayhew T. Mapping the distributions and quantifying the labelling intensities of cell compartments by immunoelectron microscopy: progress towards a coherent set of methods. J Anat. 2011;219:647-60 pubmed publisher
    ..Here, the current state of the art is reviewed and approaches are illustrated with virtual datasets...
  8. Mayhew T. Quantitative description of the spatial arrangement of organelles in a polarised secretory epithelial cell: the salivary gland acinar cell. J Anat. 1999;194 ( Pt 2):279-85 pubmed
    ..These findings provide quantitative confirmation of the polarised arrangement of zymogen granules within acinar cells and further support for the relative invariance of biological organisation between subjects...