T M Mayhew
Affiliation: University of Nottingham
Mayhew T. Quantifying immunogold localization on electron microscopic thin sections: a compendium of new approaches for plant cell biologists. J Exp Bot. 2011;62:4101-13 pubmed publisher
..The various methods are illustrated with worked examples based on empirical and synthetic data and will be of practical benefit to those applying single or multiple immunogold labelling in their research. ..
Mayhew T. Quantitative immunocytochemistry at the ultrastructural level: a stereology-based approach to molecular nanomorphomics. Cell Tissue Res. 2015;360:43-59 pubmed publisher
..Although developed for quantifying colloidal gold particles, the same methods can in principle be used to quantify other electron-dense punctate nanoparticles, including quantum dots. ..
Mayhew T. Morphomics: An integral part of systems biology of the human placenta. Placenta. 2015;36:329-40 pubmed publisher
..These considerations are relevant to future study contributions to the Human Placenta Project. ..
Mayhew T, Bowles C, Yucel F. Hypobaric hypoxia and villous trophoblast: evidence that human pregnancy at high altitude (3600 m) perturbs epithelial turnover and coagulation-fibrinolysis in the intervillous space. Placenta. 2002;23:154-62 pubmed
..The findings are partly consistent with results from in vitro studies which indicate that hypoxia stimulates proliferation of cytotrophoblast but impairs fusion into syncytium. ..
Mayhew T, Lucocq J. Developments in cell biology for quantitative immunoelectron microscopy based on thin sections: a review. Histochem Cell Biol. 2008;130:299-313 pubmed publisher
..Finally, we discuss labelling efficiency (the number of gold particles per target molecule) and describe how it can be estimated for volume- or surface-occupiers by combining stereological data with biochemical determinations. ..
Mayhew T, Lucocq J, Griffiths G. Relative labelling index: a novel stereological approach to test for non-random immunogold labelling of organelles and membranes on transmission electron microscopy thin sections. J Microsc. 2002;205:153-64 pubmed
..Contingency table analysis allows labelling distributions in different groups of cells to be compared. Protocols are described and illustrated using worked specimen examples and real data...
Mayhew T, Griffiths G, Lucocq J. Applications of an efficient method for comparing immunogold labelling patterns in the same sets of compartments in different groups of cells. Histochem Cell Biol. 2004;122:171-7 pubmed
..Its application is illustrated using datasets from immunolabelling studies designed to localise the KDEL receptor, phosphatidyl-inositol 4,5-bisphosphate, GLUT4 and rab4 at the electron microscopic level...
Mayhew T, Lucocq J. Quantifying immunogold labelling patterns of cellular compartments when they comprise mixtures of membranes (surface-occupying) and organelles (volume-occupying). Histochem Cell Biol. 2008;129:367-78 pubmed publisher
..LDs of the chosen compartments are used to calculate RLI and chi-squared values and these serve to identify those compartments in which there is preferential labelling. The methods are illustrated by synthetic and real data...
Mayhew T, Lucocq J. Multiple-labelling immunoEM using different sizes of colloidal gold: alternative approaches to test for differential distribution and colocalization in subcellular structures. Histochem Cell Biol. 2011;135:317-26 pubmed publisher
..These simple and efficient approaches should be of more immediate utility to those interested in codistribution and colocalization in multiple immunogold labelling investigations. ..