C Mackintosh

Summary

Affiliation: University of Dundee
Country: UK

Publications

  1. pmc Proteomic screen in the simple metazoan Hydra identifies 14-3-3 binding proteins implicated in cellular metabolism, cytoskeletal organisation and Ca2+ signalling
    Barbara Pauly
    Ludwig Maximilians University Munich, Germany
    BMC Cell Biol 8:31. 2007
  2. pmc Dynamic interactions between 14-3-3 proteins and phosphoproteins regulate diverse cellular processes
    Carol MacKintosh
    MRC Protein Phosphorylation Unit, School of Life Sciences, University of Dundee, Dundee DD1 5EH, Scotland, UK
    Biochem J 381:329-42. 2004
  3. ncbi request reprint Regulation of plant NR activity by reversible phosphorylation, 14-3-3 proteins and proteolysis
    C Mackintosh
    Department of Biochemistry, MSI WTB complex, University of Dundee, Scotland, United Kingdom
    Cell Mol Life Sci 58:205-14. 2001
  4. ncbi request reprint Phosphorylated nitrate reductase from spinach leaves is inhibited by 14-3-3 proteins and activated by fusicoccin
    G Moorhead
    Medical Research Council Protein Phosphorylation Unit, Department of Biochemistry, University of Dundee, Dundee DD1 4HN, UK
    Curr Biol 6:1104-13. 1996
  5. ncbi request reprint A myofibrillar protein phosphatase from rabbit skeletal muscle contains the beta isoform of protein phosphatase-1 complexed to a regulatory subunit which greatly enhances the dephosphorylation of myosin
    P Dent
    Department of Biochemistry, The University, Dundee, Scotland
    Eur J Biochem 210:1037-44. 1992
  6. pmc Identification of high levels of type 1 and type 2A protein phosphatases in higher plants
    C Mackintosh
    Department of Biochemistry, University of Dundee, Scotland, U K
    Biochem J 262:335-9. 1989
  7. ncbi request reprint Regulation of cytosolic enzymes in primary metabolism by reversible protein phosphorylation
    C Mackintosh
    MRC Protein Phosphorylation Unit, Department of Biochemistry, University of Dundee, DD1 4HN, Scotland, UK
    Curr Opin Plant Biol 1:224-9. 1998
  8. ncbi request reprint Purification of a nitrate reductase kinase from Spinacea oleracea leaves, and its identification as a calmodulin-domain protein kinase
    P Douglas
    Department of Biochemistry, University of Dundee, UK
    Planta 206:435-42. 1998
  9. ncbi request reprint Further evidence that inhibitor-2 acts like a chaperone to fold PP1 into its native conformation
    C Mackintosh
    MRC Protein Phosphorylation Unit, Department of Biochemistry, University of Dundee, UK
    FEBS Lett 397:235-8. 1996
  10. ncbi request reprint Purification of the hepatic glycogen-associated form of protein phosphatase-1 by microcystin-Sepharose affinity chromatography
    G Moorhead
    Department of Biochemistry, University of Dundee, Scotland, UK
    FEBS Lett 362:101-5. 1995

Collaborators

Detail Information

Publications30

  1. pmc Proteomic screen in the simple metazoan Hydra identifies 14-3-3 binding proteins implicated in cellular metabolism, cytoskeletal organisation and Ca2+ signalling
    Barbara Pauly
    Ludwig Maximilians University Munich, Germany
    BMC Cell Biol 8:31. 2007
    ..A diverse array of target proteins has been found in higher plants and in human cell lines including proteins involved in cellular metabolism, apoptosis, cytoskeletal organisation, secretion and Ca2+ signalling...
  2. pmc Dynamic interactions between 14-3-3 proteins and phosphoproteins regulate diverse cellular processes
    Carol MacKintosh
    MRC Protein Phosphorylation Unit, School of Life Sciences, University of Dundee, Dundee DD1 5EH, Scotland, UK
    Biochem J 381:329-42. 2004
    ....
  3. ncbi request reprint Regulation of plant NR activity by reversible phosphorylation, 14-3-3 proteins and proteolysis
    C Mackintosh
    Department of Biochemistry, MSI WTB complex, University of Dundee, Scotland, United Kingdom
    Cell Mol Life Sci 58:205-14. 2001
    ..Surprisingly, the question of how changes in NR activity actually affect the rate of nitrate assimilation is turning out to be a tough problem...
  4. ncbi request reprint Phosphorylated nitrate reductase from spinach leaves is inhibited by 14-3-3 proteins and activated by fusicoccin
    G Moorhead
    Medical Research Council Protein Phosphorylation Unit, Department of Biochemistry, University of Dundee, Dundee DD1 4HN, UK
    Curr Biol 6:1104-13. 1996
    ..Their dimeric, saddle-shaped structures support the proposal that 14-3-3 proteins are 'adaptors' linking different signalling proteins, but their precise functions are still a mystery...
  5. ncbi request reprint A myofibrillar protein phosphatase from rabbit skeletal muscle contains the beta isoform of protein phosphatase-1 complexed to a regulatory subunit which greatly enhances the dephosphorylation of myosin
    P Dent
    Department of Biochemistry, The University, Dundee, Scotland
    Eur J Biochem 210:1037-44. 1992
    ....
  6. pmc Identification of high levels of type 1 and type 2A protein phosphatases in higher plants
    C Mackintosh
    Department of Biochemistry, University of Dundee, Scotland, U K
    Biochem J 262:335-9. 1989
    ....
  7. ncbi request reprint Regulation of cytosolic enzymes in primary metabolism by reversible protein phosphorylation
    C Mackintosh
    MRC Protein Phosphorylation Unit, Department of Biochemistry, University of Dundee, DD1 4HN, Scotland, UK
    Curr Opin Plant Biol 1:224-9. 1998
    ....
  8. ncbi request reprint Purification of a nitrate reductase kinase from Spinacea oleracea leaves, and its identification as a calmodulin-domain protein kinase
    P Douglas
    Department of Biochemistry, University of Dundee, UK
    Planta 206:435-42. 1998
    ....
  9. ncbi request reprint Further evidence that inhibitor-2 acts like a chaperone to fold PP1 into its native conformation
    C Mackintosh
    MRC Protein Phosphorylation Unit, Department of Biochemistry, University of Dundee, UK
    FEBS Lett 397:235-8. 1996
    ....
  10. ncbi request reprint Purification of the hepatic glycogen-associated form of protein phosphatase-1 by microcystin-Sepharose affinity chromatography
    G Moorhead
    Department of Biochemistry, University of Dundee, Scotland, UK
    FEBS Lett 362:101-5. 1995
    ..The GL subunit binds phosphorylase a with high affinity, and is responsible for the enhanced dephosphorylation of glycogen synthase by PP1G and its allosteric inhibition by phosphorylase a...
  11. doi request reprint 14-3-3 Binding to Pim-phosphorylated Ser166 and Ser186 of human Mdm2--Potential interplay with the PKB/Akt pathway and p14(ARF)
    Nicola T Wood
    MRC Protein Phosphorylation Unit, Sir James Black Centre, College of Life Sciences, University of Dundee, Dundee DD1 5EH, Scotland, UK
    FEBS Lett 583:615-20. 2009
    ..The implications of these findings for regulation of the p53 pathway, oncogenesis and drug discovery are discussed...
  12. pmc Protein kinase C-mediated phosphorylation and activation of PDE3A regulate cAMP levels in human platelets
    Roger W Hunter
    Department of Biochemistry, School of Medical Sciences, University of Bristol, Bristol BS8 1TD, UK
    J Biol Chem 284:12339-48. 2009
    ....
  13. ncbi request reprint Phosphorylation and 14-3-3 binding of Arabidopsis 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase
    Anna Kulma
    MRC Protein Phosphorylation Unit, School of Life Sciences, MSI WTB complex, University of Dundee, Dundee DD1 5EH, Scotland, UK
    Plant J 37:654-67. 2004
    ..Nevertheless, our findings indicate the possibility that 14-3-3 binding to SnRK1-phosphorylated sites on NR and F2KP may regulate both nitrate assimilation and sucrose/starch partitioning in leaves...
  14. pmc 14-3-3s regulate fructose-2,6-bisphosphate levels by binding to PKB-phosphorylated cardiac fructose-2,6-bisphosphate kinase/phosphatase
    Mercedes Pozuelo Rubio
    MRC Protein Phosphorylation Unit, School of Life Sciences, University of Dundee, Dundee DD1 5EH, Scotland, UK
    EMBO J 22:3514-23. 2003
    ..These findings suggest that PKB-dependent binding of 14-3-3s to phospho-Ser483 of cardiac PFK-2 mediates the stimulation of glycolysis by growth factor...
  15. pmc Mice with AS160/TBC1D4-Thr649Ala knockin mutation are glucose intolerant with reduced insulin sensitivity and altered GLUT4 trafficking
    Shuai Chen
    MRC Protein Phosphorylation Unit, College of Life Sciences, University of Dundee, Dow Street, Dundee DD1 5EH, Scotland, UK
    Cell Metab 13:68-79. 2011
    ....
  16. pmc Bioinformatic and experimental survey of 14-3-3-binding sites
    Catherine Johnson
    MRC Protein Phosphorylation Unit, College of Life Sciences, University of Dundee, Dundee DD15EH, Scotland, U K
    Biochem J 427:69-78. 2010
    ..We speculate that 14-3-3 dimers may have contributed to the evolution of such families, tailoring regulatory inputs to different physiological demands...
  17. pmc Regulation of the 14-3-3-binding protein p39 by growth factors and nutrients in rat PC12 pheochromocytoma cells
    Jean E Harthill
    Medical Research Council Protein Phosphorylation Unit, School of Life Sciences, MSI WTB complex, University of Dundee, Dundee DD1 5EH, Scotland, U K
    Biochem J 368:565-72. 2002
    ..In addition, amino acid starvation induces a CHX- and MG132-sensitive pathway that targets p39 and appears to be distinct from the mechanism of regulation of 4E-BP1 and p70 S6 kinase...
  18. ncbi request reprint Phosphorylation and 14-3-3 binding of Arabidopsis trehalose-phosphate synthase 5 in response to 2-deoxyglucose
    Jean E Harthill
    MRC Protein Phosphorylation Unit, School of Life Sciences, University of Dundee, Dundee, DD1 5EH, UK
    Plant J 47:211-23. 2006
    ..We hypothesize that coordinated phosphorylation and 14-3-3 binding of nitrate reductase (NR), 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase (F2KP) and class II TPS isoforms mediate responses to signals that activate SnRK1...
  19. pmc Regulation of multisite phosphorylation and 14-3-3 binding of AS160 in response to IGF-1, EGF, PMA and AICAR
    Kathryn M Geraghty
    MRC Protein Phosphorylation Unit, College of Life Sciences, University of Dundee, Dundee DD1 5EH, Scotland, UK
    Biochem J 407:231-41. 2007
    ..Thus, although AS160 is a common target of insulin, IGF-1, EGF, PMA and AICAR, these stimuli induce distinctive patterns of phosphorylation and 14-3-3 binding, mediated by at least four protein kinases...
  20. ncbi request reprint Microcystin affinity purification of plant protein phosphatases: PP1C, PP5 and a regulatory A-subunit of PP2A
    S Meek
    Medical Research Council Protein Phosphorylation Unit, Department of Biochemistry, University of Dundee, MSI WTB complex, Dundee, UK
    FEBS Lett 457:494-8. 1999
    ..Similar to mammalian PP5,the casein phosphatase activity of plant PP5 was activated >10-fold by arachidonic acid, with half-maximal stimulation occurring at approximately 100 microM lipid...
  21. ncbi request reprint Affinity purification of diverse plant and human 14-3-3-binding partners
    F C Milne
    MRC Protein Phosphorylation Unit, School of Life Sciences, University of Dundee, Dundee DD1 5EH, Scotland, UK
    Biochem Soc Trans 30:379-81. 2002
    ....
  22. ncbi request reprint The cyanobacterial toxin microcystin binds covalently to cysteine-273 on protein phosphatase 1
    R W MacKintosh
    Department of Biochemistry, University of Dundee, Scotland, UK
    FEBS Lett 371:236-40. 1995
    ..The covalent binding of MC to protein serine/threonine phosphatases explains the failure to detect this toxin post-mortem in suspected cases of MC poisoning...
  23. pmc Phosphodiesterase 3A binds to 14-3-3 proteins in response to PMA-induced phosphorylation of Ser428
    Mercedes Pozuelo Rubio
    MRC Protein Phosphorylation Unit, Faculty of Life Sciences, University of Dundee, Dundee DD1 5EH, UK
    Biochem J 392:163-72. 2005
    ..An additional observation was that the cytoskeletal cross-linker protein plectin-1 coimmunoprecipitated with PDE3A independently of 14-3-3 binding...
  24. ncbi request reprint Complementary regulation of TBC1D1 and AS160 by growth factors, insulin and AMPK activators
    Shuai Chen
    MRC Protein Phosphorylation Unit, College of Life Sciences, University of Dundee, Dundee DD1 5EH, Scotland, UK
    Biochem J 409:449-59. 2008
    ..These findings suggest that TBC1D1 and AS160 may have complementary roles in regulating vesicle trafficking in response to insulin and AMPK-activating stimuli in skeletal muscle...
  25. pmc 14-3-3-affinity purification of over 200 human phosphoproteins reveals new links to regulation of cellular metabolism, proliferation and trafficking
    Mercedes Pozuelo Rubio
    MRC Protein Phosphorylation Unit, School of Life Sciences, University of Dundee, Dundee DD1 5EH, Scotland, UK
    Biochem J 379:395-408. 2004
    ..Overall, our findings suggest that 14-3-3s may be central to integrating the regulation of biosynthetic metabolism, cell proliferation, survival, and other processes in human cells...
  26. pmc Differential 14-3-3 affinity capture reveals new downstream targets of phosphatidylinositol 3-kinase signaling
    Fanny Dubois
    Medical Research Council Protein Phosphorylation Unit, College of Life Sciences, University of Dundee, Dundee DD1 5EH, Scotland, United Kingdom
    Mol Cell Proteomics 8:2487-99. 2009
    ..Differential 14-3-3 capture provides a powerful approach to defining downstream regulatory mechanisms for specific signaling pathways...
  27. doi request reprint Differential regulation of NHE1 phosphorylation and glucose uptake by inhibitors of the ERK pathway and p90RSK in 3T3-L1 adipocytes
    Shuai Chen
    MRC Protein Phosphorylation Unit, College of Life Sciences, University of Dundee, Dundee DD1 5EH, Scotland, UK
    Cell Signal 21:1984-93. 2009
    ..Taken together, we propose that BI-D1870 and SL0101 might exert their inhibitory effects on glucose uptake in 3T3-L1 adipocytes at least partially through a p90RSK dependent step after GLUT4 becomes associated with the plasma membrane...
  28. ncbi request reprint PRAS40 is a target for mammalian target of rapamycin complex 1 and is required for signaling downstream of this complex
    Bruno D Fonseca
    Department of Biochemistry and Molecular Biology, University of British Columbia, Vancouver, British Columbia V6T 1Z3, Canada
    J Biol Chem 282:24514-24. 2007
    ..However, this has no effect on the phosphorylation of Akt or TSC2 (an Akt substrate). These data place PRAS40 downstream of mTORC1 but upstream of its effectors, such as S6K1 and 4E-BP1...
  29. ncbi request reprint Affinity methods for phosphorylation-dependent interactions
    Greg Moorhead
    Department of Biological Sciences, University of Calgary, Calgary, Alberta, Canada
    Methods Mol Biol 261:469-78. 2004
    ..In principle, these methods could be adapted to characterize other types of protein-protein interaction that depend on covalent modification of target sites...
  30. ncbi request reprint Metabolic enzymes as targets for 14-3-3 proteins
    Steven C Huber
    USDA ARS and Department of Crop Science, North Carolina State University, Raleigh, NC 27695 7631, USA
    Plant Mol Biol 50:1053-63. 2002
    ....