L M Machesky

Summary

Affiliation: University of Birmingham
Country: UK

Publications

  1. pmc Abp1p and cortactin, new "hand-holds" for actin
    I M Olazabal
    School of Biosciences, Division of Molecular Cell Biology, University of Birmingham, Birmingham B15 2TT, United Kingdom
    J Cell Biol 154:679-82. 2001
  2. pmc Studies on the actin-binding protein HS1 in platelets
    Steven G Thomas
    School of Biosciences, University of Birmingham, Edgbaston, Birmingham, B15 2TT, UK
    BMC Cell Biol 8:46. 2007
  3. pmc Inclusion of Scar/WAVE3 in a similar complex to Scar/WAVE1 and 2
    Craig F Stovold
    School of Biosciences, The University of Birmingham, Edgbaston, Birmingham, B15 2TT, UK
    BMC Cell Biol 6:11. 2005
  4. pmc Phenotypes of myopathy-related actin mutants in differentiated C2C12 myotubes
    Friederike S Bathe
    School of Biosciences, Division of Molecular Cell Biology, University of Birmingham, Birmingham B15 2TT, UK
    BMC Cell Biol 8:2. 2007
  5. ncbi request reprint Putting on the brakes: a negative regulatory function for Ena/VASP proteins in cell migration
    L M Machesky
    School of Biosciences, Division of Molecular and Cell Biology, University of Birmingham, United Kingdom
    Cell 101:685-8. 2000
  6. ncbi request reprint MIM: a multifunctional scaffold protein
    Laura M Machesky
    School of Biosciences, University of Birmingham, Birmingham, B15 2TT, UK
    J Mol Med (Berl) 85:569-76. 2007
  7. ncbi request reprint MyosinIIa contractility is required for maintenance of platelet structure during spreading on collagen and contributes to thrombus stability
    S D J Calaminus
    Centre for Cardiovascular Sciences, Institute of Biomedical Research, The Medical School, University of Birmingham, Birmingham, UK
    J Thromb Haemost 5:2136-45. 2007
  8. ncbi request reprint Identification of a novel, actin-rich structure, the actin nodule, in the early stages of platelet spreading
    S D J Calaminus
    Centre for Cardiovascular Sciences, Institute of Biomedical Research, The Medical School, University of Birmingham, Birmingham, UK
    J Thromb Haemost 6:1944-52. 2008
  9. pmc A major role for Scar/WAVE-1 downstream of GPVI in platelets
    S D J Calaminus
    Centre for Cardiovascular Sciences, Institute of Biomedical Research, The Medical School, University of Birmingham, Birmingham, UK
    J Thromb Haemost 5:535-41. 2007
  10. ncbi request reprint Involvement of Rac in actin cytoskeleton rearrangements induced by MIM-B
    Guillaume Bompard
    School of Biosciences, Division of Molecular Cell Biology, University of Birmingham, Edgbaston, Birmingham, B15 2TT UK
    J Cell Sci 118:5393-403. 2005

Collaborators

Detail Information

Publications52

  1. pmc Abp1p and cortactin, new "hand-holds" for actin
    I M Olazabal
    School of Biosciences, Division of Molecular Cell Biology, University of Birmingham, Birmingham B15 2TT, United Kingdom
    J Cell Biol 154:679-82. 2001
    ..Abp1p, a yeast protein involved in endocytosis, and cortactin, a mammalian src substrate, both enhance the ability of the Arp2/3 complex to assemble branched actin filament networks...
  2. pmc Studies on the actin-binding protein HS1 in platelets
    Steven G Thomas
    School of Biosciences, University of Birmingham, Edgbaston, Birmingham, B15 2TT, UK
    BMC Cell Biol 8:46. 2007
    ..The Arp2/3 complex is highly regulated by a number of actin-binding proteins including the haematopoietic-specific protein HS1 and its homologue cortactin. The present study investigates the role of HS1 in platelets using HS1-/- mice...
  3. pmc Inclusion of Scar/WAVE3 in a similar complex to Scar/WAVE1 and 2
    Craig F Stovold
    School of Biosciences, The University of Birmingham, Edgbaston, Birmingham, B15 2TT, UK
    BMC Cell Biol 6:11. 2005
    ..These proteins have been characterised as major regulators of lamellipodia formation downstream of Rac activation and as members of large protein complexes...
  4. pmc Phenotypes of myopathy-related actin mutants in differentiated C2C12 myotubes
    Friederike S Bathe
    School of Biosciences, Division of Molecular Cell Biology, University of Birmingham, Birmingham B15 2TT, UK
    BMC Cell Biol 8:2. 2007
    ..Results were compared with phenotypes in patient skeletal muscle fibres and with previous expression studies in fibroblasts and C2C12 myoblasts/myotubes...
  5. ncbi request reprint Putting on the brakes: a negative regulatory function for Ena/VASP proteins in cell migration
    L M Machesky
    School of Biosciences, Division of Molecular and Cell Biology, University of Birmingham, United Kingdom
    Cell 101:685-8. 2000
  6. ncbi request reprint MIM: a multifunctional scaffold protein
    Laura M Machesky
    School of Biosciences, University of Birmingham, Birmingham, B15 2TT, UK
    J Mol Med (Berl) 85:569-76. 2007
    ..How this multifunctional protein and its close relative ABBA-1 regulate cellular behaviour is still very much an open question...
  7. ncbi request reprint MyosinIIa contractility is required for maintenance of platelet structure during spreading on collagen and contributes to thrombus stability
    S D J Calaminus
    Centre for Cardiovascular Sciences, Institute of Biomedical Research, The Medical School, University of Birmingham, Birmingham, UK
    J Thromb Haemost 5:2136-45. 2007
    ..MyosinIIa phosphorylation underlies platelet rounding and stress fiber formation...
  8. ncbi request reprint Identification of a novel, actin-rich structure, the actin nodule, in the early stages of platelet spreading
    S D J Calaminus
    Centre for Cardiovascular Sciences, Institute of Biomedical Research, The Medical School, University of Birmingham, Birmingham, UK
    J Thromb Haemost 6:1944-52. 2008
    ..In the present study, we report the identification of a novel actin-rich structure, termed an actin nodule, which appears prior to lamellipodia and stress fibre formation...
  9. pmc A major role for Scar/WAVE-1 downstream of GPVI in platelets
    S D J Calaminus
    Centre for Cardiovascular Sciences, Institute of Biomedical Research, The Medical School, University of Birmingham, Birmingham, UK
    J Thromb Haemost 5:535-41. 2007
    ..Rac mediates actin assembly via Scar/WAVE, a family of scaffolding proteins that direct actin reorganization by relaying signals from Rac to the Arp2/3 complex...
  10. ncbi request reprint Involvement of Rac in actin cytoskeleton rearrangements induced by MIM-B
    Guillaume Bompard
    School of Biosciences, Division of Molecular Cell Biology, University of Birmingham, Edgbaston, Birmingham, B15 2TT UK
    J Cell Sci 118:5393-403. 2005
    ..Thus, MIM-B is unlikely to be a metastasis suppressor but acts as a scaffold protein that interacts with Rac, actin and actin-associated proteins to modulate lamellipodia formation...
  11. pmc Involvement of the Arp2/3 complex and Scar2 in Golgi polarity in scratch wound models
    Juana Magdalena
    School of Biosciences, University of Birmingham, Edgbaston, United Kingdom
    Mol Biol Cell 14:670-84. 2003
    ..Furthermore, in NIH 3T3 cells, Scar2 and the Arp2/3 complex appear to be involved in the establishment and maintenance of Golgi polarity during directed migration...
  12. ncbi request reprint Bar domain proteins: a role in tubulation, scission and actin assembly in clathrin-mediated endocytosis
    John C Dawson
    School of Biosciences, University of Birmingham, Birmingham B15 2TT, UK
    Trends Cell Biol 16:493-8. 2006
    ..We describe three classes of BAR domains, BAR, N-BAR and F-BAR, providing examples of each discussing and how they function in linking membranes to the actin cytoskeleton in endocytosis...
  13. ncbi request reprint The Arp2/3 complex is essential for the actin-based motility of Listeria monocytogenes
    R C May
    Department of Biochemistry, University of Birmingham, Edgbaston, Birmingham, B15 2TT, UK
    Curr Biol 9:759-62. 1999
    ..We conclude that the Arp2/3 complex is a host-cell factor essential for the actin-based motility of L. monocytogenes, suggesting that it plays a pivotal role in regulating the actin cytoskeleton...
  14. ncbi request reprint Plagiarism and pathogenesis: common themes in actin remodeling
    R C May
    School of Biosciences, University of Birmingham, Egbaston, United Kingdom
    Dev Cell 1:317-8. 2001
    ..The involvement of Nck in pedestal formation by EPEC highlights the similar strategies adopted by this bacterium and the Vaccinia virus to hijack the host cell's cytoskeleton...
  15. ncbi request reprint Evaluation of the role of platelet integrins in fibronectin-dependent spreading and adhesion
    O J T McCarty
    Centre for Cardiovascular Sciences, The Institute of Biomedical Research, Division of Medical Sciences, The Medical School, University of Birmingham, Edgbaston, Birmingham B15 2TT, UK
    J Thromb Haemost 2:1823-33. 2004
    ..These results highlight a possible role for fibronectin in supporting thrombus formation...
  16. ncbi request reprint The Arp2/3 complex: a multifunctional actin organizer
    L M Machesky
    Department of Biochemistry University of Birmingham Edgbaston Birmingham B15 2TT UK
    Curr Opin Cell Biol 11:117-21. 1999
    ..Clearly, the complex has a central role in actin cytoskeletal function and will be the subject of much research in the coming years...
  17. pmc Rac1 is essential for platelet lamellipodia formation and aggregate stability under flow
    Owen J T McCarty
    Centre for Cardiovascular Sciences, The Institute of Biomedical Research, School of Biosciences, University of Birmingham, United Kingdom
    J Biol Chem 280:39474-84. 2005
    ....
  18. pmc Arp2/3 complex activity in filopodia of spreading cells
    Simon A Johnston
    University of Birmingham School of Biosciences, Edgbaston, Birmingham, UK
    BMC Cell Biol 9:65. 2008
    ..The interplay between filopodial and lamellipodial protrusions is an area of much interest as it is thought to be a key determinant of how cells make motility choices...
  19. ncbi request reprint "Wunder" F-BAR domains: going from pits to vesicles
    Klaus Futterer
    School of Biosciences, University of Birmingham, Edgbaston, Birmingham, B15 2TT, UK
    Cell 129:655-7. 2007
    ..In this issue, Shimada et al. (2007) reveal the mode of action of the F-BAR domain, which deepens the initial membrane pit that forms during clathrin-mediated endocytosis...
  20. ncbi request reprint Characterisation of IRTKS, a novel IRSp53/MIM family actin regulator with distinct filament bundling properties
    Thomas H Millard
    School of Biosciences, University of Birmingham, Birmingham, B15 2TT, UK
    J Cell Sci 120:1663-72. 2007
    ..Lastly, we could not detect actin monomer sequestration by the Ct extension of IRTKS as would be expected with a conventional WH2 motif, but it did interact with actin filaments...
  21. ncbi request reprint Phagocytosis and the actin cytoskeleton
    R C May
    School of Biosciences, University of Birmingham, Edgbaston, Birmingham, B15 2TT, UK
    J Cell Sci 114:1061-77. 2001
    ..Similarly, a wide diversity of 'effector molecules' are now implicated in actin-remodelling downstream of these receptors...
  22. pmc MIM-B, a putative metastasis suppressor protein, binds to actin and to protein tyrosine phosphatase delta
    Jacquelyn A Woodings
    School of Biosciences, University of Birmingham, Edgbaston, Birmingham B15 2TT, UK
    Biochem J 371:463-71. 2003
    ..We postulate that MIM-B may be a regulator of actin assembly downstream of tyrosine kinase signalling and that this activity may explain the involvement of MIM in the metastasis of cancer cells...
  23. ncbi request reprint Regulation of WASP: PIP2 Pipped by Toca-1?
    Robert H Insall
    School of Biosciences, University of Birmingham, Birmingham B15 2TT, United Kingdom
    Cell 118:140-1. 2004
    ..This discovery changes our picture of how small GTPases and multiple other signals converge to stimulate actin polymerization and cell motility...
  24. ncbi request reprint von Willebrand factor mediates platelet spreading through glycoprotein Ib and alpha(IIb)beta3 in the presence of botrocetin and ristocetin, respectively
    O J T McCarty
    Centre for Cardiovascular Sciences, University of Birmingham, Birmingham, UK
    J Thromb Haemost 4:1367-78. 2006
    ..To accomplish this, VWF binds to two distinct platelet receptors: glycoprotein (GP)Ib-IX-V and integrin alpha(IIb)beta3...
  25. ncbi request reprint Identification of the insulin-responsive tyrosine phosphorylation sites on IRSp53
    Man Yeung Heung
    School of Biosciences, University of Birmingham, Edgbaston, Birmingham B15 2TT, UK
    Eur J Cell Biol 87:699-708. 2008
    ..Furthermore, the tyrosine phosphorylation status does not appear to affect the cell morphology and production of filopod-like structures upon expression of IRSp53...
  26. pmc Signalling to actin assembly via the WASP (Wiskott-Aldrich syndrome protein)-family proteins and the Arp2/3 complex
    Thomas H Millard
    School of Biosciences, University of Birmingham, Edgbaston, Birmingham, B15 2TT, UK
    Biochem J 380:1-17. 2004
    ..Emphasis will be placed on recent developments in the field that have furthered our understanding of actin dynamics and cell motility...
  27. ncbi request reprint Expression of WASP and Scar1/WAVE1 actin-associated proteins is differentially modulated during differentiation of HL-60 cells
    Sophie Launay
    School of Biosciences, University of Birmingham, Edgbaston, Birmingham, United Kingdom
    Cell Motil Cytoskeleton 54:274-85. 2003
    ..These observations show for the first time that modulation of Scar1 and WASP expression is a component of the differentiation program of myeloid precursors and indicate that WASP and Scar1 have different roles in mature myeloid cells...
  28. ncbi request reprint Myopathy mutations in alpha-skeletal-muscle actin cause a range of molecular defects
    Celine F Costa
    School of Biosciences, Division of Molecular Cell Biology, University of Birmingham, B15 2TT, UK
    J Cell Sci 117:3367-77. 2004
    ..Overall, we found a range of defects and behaviors of the mutants in vitro and in cultured cells, paralleling the complexity of actin-based muscle myopathy phenotypes...
  29. ncbi request reprint Microtubule involvement in NIH 3T3 Golgi and MTOC polarity establishment
    Juana Magdalena
    School of Biosciences, University of Birmingham, Edgbaston, Birmingham B15 2TT, UK
    J Cell Sci 116:743-56. 2003
    ..In addition, this study indicates that MTOC and Golgi polarization events are separately controlled...
  30. ncbi request reprint The Wiskott-Aldrich syndrome protein (WASP) family
    T H Millard
    School of Biosciences, Division of Molecular Cell Biology, University of Birmingham, UK B15 2TT
    Trends Biochem Sci 26:198-9. 2001
  31. ncbi request reprint Congenital myopathies: diseases of the actin cytoskeleton
    Emilie Clarkson
    School of Biosciences, University of Birmingham, Birmingham, B15 2TT, UK
    J Pathol 204:407-17. 2004
    ..There are no existing cures for congenital myopathies, although improvements can be made to both the standard of living and the life expectancy of the patient through various therapies...
  32. pmc N-WASP involvement in dorsal ruffle formation in mouse embryonic fibroblasts
    John A Legg
    School of Biosciences, The University of Birmingham, Birmingham, Edgbaston, West Midlands, B15 2TT, United Kingdom
    Mol Biol Cell 18:678-87. 2007
    ..We hypothesize that N-WASP and Arp2/3 complex are part of a multiprotein assembly important for the generation of dorsal ruffles and that Scar1 and Scar2 are dispensable for this process...
  33. ncbi request reprint Rho-kinase and myosin-II control phagocytic cup formation during CR, but not FcgammaR, phagocytosis
    Isabel M Olazabal
    School of Biosciences, University of Birmingham, Edgbaston, B15 2TT, Birmingham, United Kingdom
    Curr Biol 12:1413-18. 2002
    ..While myosins have been previously implicated in FcgammaR phagocytosis, to our knowledge, this is the first demonstration of a role for myosin-II in CR phagocytosis...
  34. ncbi request reprint Identification and characterisation of a novel human isoform of Arp2/3 complex subunit p16-ARC/ARPC5
    Thomas H Millard
    School of Biosciences, University of Birmingham, Edgbaston, Birmingham, United Kingdom
    Cell Motil Cytoskeleton 54:81-90. 2003
    ..Our data show for the first time that mammalian cells contain multiple forms of the Arp2/3 complex...
  35. pmc Spred2 interaction with the late endosomal protein NBR1 down-regulates fibroblast growth factor receptor signaling
    Faraz K Mardakheh
    Cancer Research UK Growth Factor Group, University of Birmingham, Birmingham B15 2TT, England, UK
    J Cell Biol 187:265-77. 2009
    ..Our findings suggest a critical function for NBR1 in the regulation of receptor trafficking and provide a mechanism for down-regulation of signaling by Spred2 via NBR1...
  36. pmc Structural basis of filopodia formation induced by the IRSp53/MIM homology domain of human IRSp53
    Thomas H Millard
    School of Biosciences, The University of Birmingham, Edgbaston, Birmingham, UK
    EMBO J 24:240-50. 2005
    ..This study promotes an expanded view of IRSp53 as an actin regulator that integrates scaffolding and effector functions...
  37. ncbi request reprint The WASP-Arp2/3 pathway: genetic insights
    Maria K Vartiainen
    Cancer Research UK, London Research Institute, Lincoln s Inn Fields Laboratories, Transcription Laboratory, London, WC2A 3PX, UK
    Curr Opin Cell Biol 16:174-81. 2004
    ..It has also become clear that precise regulation of both Scar and WASP activity is of the utmost importance for their physiological functions...
  38. ncbi request reprint Sharks' teeth and dunes
    Laura M Machesky
    Nature 417:494-7. 2002
  39. ncbi request reprint Lamellipodia and filopodia in metastasis and invasion
    Laura M Machesky
    The Beatson Institute for Cancer Research, Garscube Estate, Switchback Road, Bearsden, Glasgow G61 1BD, United Kingdom
    FEBS Lett 582:2102-11. 2008
    ..As key proteins are uncovered which regulate the assembly of actin-based structures, these need to be considered in light of whether they represent potential invasion and metastasis proteins...
  40. ncbi request reprint MRL proteins: leading Ena/VASP to Ras GTPases
    John A Legg
    Nat Cell Biol 6:1015-7. 2004
  41. ncbi request reprint Linked regulation of motility and integrin function in activated migrating neutrophils revealed by interference in remodelling of the cytoskeleton
    Stephen I Anderson
    Department of Physiology, The Medical School, The University of Birmingham, Birmingham B15 2TT, UK
    Cell Motil Cytoskeleton 54:135-46. 2003
    ..They also suggest a role of the Arp2/3 complex and WASP-family in co-ordinating actin polymerisation and integrin function in migrating neutrophils...
  42. pmc Interaction between Tiam1 and the Arp2/3 complex links activation of Rac to actin polymerization
    Jean Paul ten Klooster
    The Netherlands Cancer Institute, Division of Cell Biology, Plesmanlaan 121, 1066 CX Amsterdam, The Netherlands
    Biochem J 397:39-45. 2006
    ..This 'self-amplifying' signalling module involving Tiam1, Rac and the Arp2/3 complex could thus drive actin polymerization at specific sites in cells that are required for dynamic morphological changes...
  43. ncbi request reprint Identification of the p16-Arc subunit of the Arp 2/3 complex as a substrate of MAPK-activated protein kinase 2 by proteomic analysis
    Saurabh Singh
    Department of Biochemistry and Molecular Biology, Medicine, and Pharmacology, University of Louisville, KY 40202, USA
    J Biol Chem 278:36410-7. 2003
    ..The ability of MAPKAPK2 to phosphorylate one isoform of p16-Arc suggests a possible mechanism by which the p38 MAPK cascade regulates remodeling of the actin cytoskeleton...
  44. ncbi request reprint Actin dynamics at sites of extracellular matrix degradation
    Massimiliano Baldassarre
    Department of Cell Biology and Oncology, Consorzio Mario Negri Sud, Via Nazionale 8a, I 66030 S Maria Imbaro, Chieti, Italy
    Eur J Cell Biol 85:1217-31. 2006
    ..In conclusion, invadopodia-associated actin comets are a striking example of consistently arising, spontaneous expression of actin-driven propulsion events that also represent a valuable experimental paradigm...
  45. pmc Spatially distinct binding of Cdc42 to PAK1 and N-WASP in breast carcinoma cells
    Maddy Parsons
    Randall Centre, King s College London, 3rd Floor, New Hunt s House, Guy s Medical School Campus, London SE1 1UL, United Kingdom
    Mol Cell Biol 25:1680-95. 2005
    ..These data reveal for the first time distinct spatial association patterns between Cdc42 and its key effector proteins controlling cytoskeletal remodeling...
  46. ncbi request reprint CrkL directs ASAP1 to peripheral focal adhesions
    Atsushi Oda
    Laboratory of Environmental Biology, Department of Preventive Medicine, Hokkaido University School of Medicine, Sapporo 060 8638, Japan
    J Biol Chem 278:6456-60. 2003
    ..An SH2-mutated CrkL, which cannot localize at focal adhesions, failed to recruit ASAP1 into focal adhesions. Thus, CrkL appears to be a lynchpin between ASAP1 and peripheral focal adhesions...
  47. ncbi request reprint WAVE/Scars in platelets
    Atsushi Oda
    Laboratory of Environmental Biology, Department of Preventive Medicine, Hokkaido University School of Medicine, Kitaku, Sapporo, 060 8638, Japan
    Blood 105:3141-8. 2005
    ..These data suggest that the 3 WAVE isoforms exhibit common and distinct features and may potentially be involved in the regulation of actin cytoskeleton in platelets...
  48. ncbi request reprint Arabidopsis group Ie formins localize to specific cell membrane domains, interact with actin-binding proteins and cause defects in cell expansion upon aberrant expression
    Michael J Deeks
    The Integrative Cell Biology Laboratory, School of Biological and Biomedical Sciences, University of Durham, Science Laboratories, South Road, Durham DH1 3LE, UK
    New Phytol 168:529-40. 2005
    ..Taken together, these data suggest that the group Ie formins act with profilin to regulate actin polymerization at specific sites associated with the cell membrane...
  49. ncbi request reprint Src-dependent phosphorylation of Scar1 promotes its association with the Arp2/3 complex
    Hazel Ardern
    The Beatson Institute for Cancer Research, Cancer Research UK Beatson Laboratories, Bearsden, Glasgow G61 1BD, United Kingdom
    Cell Motil Cytoskeleton 63:6-13. 2006
    ..Thus, Src may act as an intermediary to regulate the activity of the Arp2/3 complex in response to external stimuli, via modulation of its interaction with WAVE/Scar proteins...
  50. ncbi request reprint Cell structure and dynamics
    Laura M Machesky
    Curr Opin Cell Biol 15:2-5. 2003
  51. ncbi request reprint Enhancement of ATRA-induced cell differentiation by inhibition of calcium accumulation into the endoplasmic reticulum: cross-talk between RAR alpha and calcium-dependent signaling
    Sophie Launay
    U348 Institut National de la Santé et de la Recherche Médicale, IFR Circulation Lariboisiere, Hopital Lariboisiere, Paris, France
    Blood 101:3220-8. 2003
    ..By linking calcium to ATRA-dependent signaling, our data open new avenues in the understanding of the mechanisms of differentiation-induction therapy of leukemia...