J A Littlechild

Summary

Affiliation: University of Exeter
Country: UK

Publications

  1. ncbi request reprint Structural and functional comparisons between vanadium haloperoxidase and acid phosphatase enzymes
    Jennifer Littlechild
    Schools of Chemistry and Biological Sciences, University of Exeter, Stocker Road, UK
    J Mol Recognit 15:291-6. 2002
  2. pmc Structural studies of Pseudomonas and Chromobacterium ω-aminotransferases provide insights into their differing substrate specificity
    Christopher Sayer
    Henry Wellcome Building for Biocatalysis, Biosciences, College of Life and Environmental Sciences, University of Exeter, Exeter, England
    Acta Crystallogr D Biol Crystallogr 69:564-76. 2013
  3. pmc Crystallization and preliminary X-ray diffraction analysis of omega-amino acid:pyruvate transaminase from Chromobacterium violaceum
    Christopher Sayer
    Henry Wellcome Building for Biocatalysis, School of Biosciences, University of Exeter, Exeter EX4 4QD, England
    Acta Crystallogr Sect F Struct Biol Cryst Commun 63:117-9. 2007
  4. doi request reprint Thermophilic archaeal enzymes and applications in biocatalysis
    Jennifer A Littlechild
    Exeter Biocatalysis Centre, Henry Wellcome Building for Biocatalysis, Biosciences, College of Life and Environmental Sciences, University of Exeter, Stocker Road, Exeter EX4 4QD, UK
    Biochem Soc Trans 39:155-8. 2011
  5. doi request reprint Vanadium containing bromoperoxidase--insights into the enzymatic mechanism using X-ray crystallography
    Jennifer Littlechild
    School of Biosciences, University of Exeter, Exeter, UK
    J Inorg Biochem 103:617-21. 2009
  6. ncbi request reprint Hyperthermophilic dehydrogenase enzymes
    J A Littlechild
    School of Biological Science and Chemistry, Exeter Biocatalysis Centre, Stocker Road, Exeter EX4 4QD, U K
    Biochem Soc Trans 32:255-8. 2004
  7. ncbi request reprint Crystal structure of the glyceraldehyde-3-phosphate dehydrogenase from the hyperthermophilic archaeon Sulfolobus solfataricus
    M N Isupov
    Schools of Chemistry and Biological Sciences, University of Exeter, Stocker Road, Exeter, EX4 4QD, UK
    J Mol Biol 291:651-60. 1999
  8. ncbi request reprint Characterization, crystallization and preliminary X-ray investigation of glyceraldehyde-3-phosphate dehydrogenase from the hyperthermophilic archaeon Sulfolobus solfataricus
    T M Fleming
    Departments of Chemistry and Biological Sciences, University of Exeter, Stocker Road, Exeter EX4 4QD, England
    Acta Crystallogr D Biol Crystallogr 54:671-4. 1998
  9. ncbi request reprint The structure of human liver fructose-1,6-bisphosphate aldolase
    A R Dalby
    School of Chemistry, University of Exeter, Stocker Road, Exeter EX4 4QD, England
    Acta Crystallogr D Biol Crystallogr 57:1526-33. 2001
  10. ncbi request reprint Crystallization and preliminary X-ray analysis of a gamma-lactamase
    I S Gonsalvez
    School of Chemistry, University of Exeter, Exeter EX4 4QD, England
    Acta Crystallogr D Biol Crystallogr 57:284-6. 2001

Collaborators

  • Michail N Isupov
  • I Polikarpov
  • S Waddell
  • G Obmolova
  • Don A Cowan
  • E Schroder
  • Christopher Sayer
  • Esther Garcia-Rodriguez
  • Takashi Ohshiro
  • E E Coupe
  • A M Hickey
  • Yoshikazu Izumi
  • Aaron Westlake
  • N A Turner
  • A R Dalby
  • I S Gonsalvez
  • P Watts
  • L Marle
  • M G Smyth
  • A P Fosberry
  • R M Hall
  • G M Greenway
  • T McCreedy
  • T M Fleming
  • Toshiaki Aibara
  • Takushi Kobayashi
  • Yasuaki Iida
  • J McHarg
  • A Dalby
  • D R Tolan
  • E N Vulfson
  • A T Yagnik
  • D J Gaskin
  • C E Jones
  • P W Piper

Detail Information

Publications20

  1. ncbi request reprint Structural and functional comparisons between vanadium haloperoxidase and acid phosphatase enzymes
    Jennifer Littlechild
    Schools of Chemistry and Biological Sciences, University of Exeter, Stocker Road, UK
    J Mol Recognit 15:291-6. 2002
    ..The detailed active site architecture for enzymes that recognize and use either vanadium or phosphate will be discussed in relation to their proposed enzymatic mechanism...
  2. pmc Structural studies of Pseudomonas and Chromobacterium ω-aminotransferases provide insights into their differing substrate specificity
    Christopher Sayer
    Henry Wellcome Building for Biocatalysis, Biosciences, College of Life and Environmental Sciences, University of Exeter, Exeter, England
    Acta Crystallogr D Biol Crystallogr 69:564-76. 2013
    ..It appears that the different rigidity of the protein scaffold contributes to the substrate specificity observed for the two ω-aminotransferases...
  3. pmc Crystallization and preliminary X-ray diffraction analysis of omega-amino acid:pyruvate transaminase from Chromobacterium violaceum
    Christopher Sayer
    Henry Wellcome Building for Biocatalysis, School of Biosciences, University of Exeter, Exeter EX4 4QD, England
    Acta Crystallogr Sect F Struct Biol Cryst Commun 63:117-9. 2007
    ..9, b = 61.9, c = 63.9 A, alpha = 71.9, beta = 87.0, gamma = 74.6 degrees . Data were also collected to 1.95 A from a second triclinic crystal form. The structure has been solved using the molecular-replacement method...
  4. doi request reprint Thermophilic archaeal enzymes and applications in biocatalysis
    Jennifer A Littlechild
    Exeter Biocatalysis Centre, Henry Wellcome Building for Biocatalysis, Biosciences, College of Life and Environmental Sciences, University of Exeter, Stocker Road, Exeter EX4 4QD, UK
    Biochem Soc Trans 39:155-8. 2011
    ..The L-2-haloacid dehalogenase has applications both in biocatalysis and in bioremediation. All of these enzymes have increased thermostability over their mesophilic counterparts...
  5. doi request reprint Vanadium containing bromoperoxidase--insights into the enzymatic mechanism using X-ray crystallography
    Jennifer Littlechild
    School of Biosciences, University of Exeter, Exeter, UK
    J Inorg Biochem 103:617-21. 2009
    ..These results have increased our understanding of the mechanism of the vanadium bromoperoxidases and have demonstrated that the substrate and bromide are specifically bound to the enzyme active site...
  6. ncbi request reprint Hyperthermophilic dehydrogenase enzymes
    J A Littlechild
    School of Biological Science and Chemistry, Exeter Biocatalysis Centre, Stocker Road, Exeter EX4 4QD, U K
    Biochem Soc Trans 32:255-8. 2004
    ....
  7. ncbi request reprint Crystal structure of the glyceraldehyde-3-phosphate dehydrogenase from the hyperthermophilic archaeon Sulfolobus solfataricus
    M N Isupov
    Schools of Chemistry and Biological Sciences, University of Exeter, Stocker Road, Exeter, EX4 4QD, UK
    J Mol Biol 291:651-60. 1999
    ..There is a relocation of the active-site residues within the catalytic domain of the enzyme. The thermostability of the S. solfataricus enzyme can be attributed to a combination of an ion pair cluster and an intrasubunit disulphide bond...
  8. ncbi request reprint Characterization, crystallization and preliminary X-ray investigation of glyceraldehyde-3-phosphate dehydrogenase from the hyperthermophilic archaeon Sulfolobus solfataricus
    T M Fleming
    Departments of Chemistry and Biological Sciences, University of Exeter, Stocker Road, Exeter EX4 4QD, England
    Acta Crystallogr D Biol Crystallogr 54:671-4. 1998
    ..4 A with a space group of P43212 or P41212. A native data set has been collected to 2.4 A using synchrotron radiation and cryocooling...
  9. ncbi request reprint The structure of human liver fructose-1,6-bisphosphate aldolase
    A R Dalby
    School of Chemistry, University of Exeter, Stocker Road, Exeter EX4 4QD, England
    Acta Crystallogr D Biol Crystallogr 57:1526-33. 2001
    ..Sequence information is projected onto the new crystal structure in order to indicate how these mutations bring about reduced enzyme activity and affect structural stability...
  10. ncbi request reprint Crystallization and preliminary X-ray analysis of a gamma-lactamase
    I S Gonsalvez
    School of Chemistry, University of Exeter, Exeter EX4 4QD, England
    Acta Crystallogr D Biol Crystallogr 57:284-6. 2001
    ..0, b = 93.2, c = 152.4 A, alpha = 104.3, beta = 92.6, gamma = 108.5 degrees, and diffract to 2 A resolution using synchrotron radiation. Native data from these crystals have been collected to 2.4 A...
  11. ncbi request reprint Natural methods of protein stabilization: thermostable biocatalysts
    J A Littlechild
    School of Biosciences, Henry Wellcome Building for Biocatalysis, University of Exeter, Stocker Road, Exeter EX4 4QD, U K
    Biochem Soc Trans 35:1558-63. 2007
    ..This is illustrated with several different classes of enzyme that have been studied at Exeter. These include alcohol dehydrogenase, aminoacylase, pyroglutamyl carboxypeptidase, gamma-lactamase, dehalogenase and lysophospholipase...
  12. ncbi request reprint Enantioselectivity of recombinant Rhizomucor miehei lipase in the ring opening of oxazolin-5(4H)-ones
    N A Turner
    Institute of Food Research, Norwich Research Park, Colney, Norwich, NR4 7UA, and Schools of Chemistry and Biological Sciences, Exeter University, Exeter EX4 4QD, UK
    Protein Eng 14:269-78. 2001
    ..In this paper we report the construction, purification and enantioselectivity of two recombinant Rhizomucor miehei lipases which were designed to check the validity of such a model in reactions of ring opening of oxazolin-5(4H)-ones...
  13. ncbi request reprint Crystal structure of decameric 2-Cys peroxiredoxin from human erythrocytes at 1.7 A resolution
    E Schroder
    Schools of Chemistry and Biological Sciences, University of Exeter, Exeter, EX4 4QD, UK
    Structure 8:605-15. 2000
    ..Thioredoxin peroxidase B (TPx-B), a 2-Cys type II Prx from erythrocytes, promotes potassium efflux and down-regulates apoptosis and the recruitment of monocytes by endothelial tissue...
  14. ncbi request reprint Studies with type I aldolase to understand fructose intolerance and combat parasitic disease
    A Dalby
    Department of Chemistry, University of Exeter, UK
    J Pharm Pharmacol 48:214-7. 1996
    ..X-ray crystallographic data is used with molecular modelling techniques to investigate the structural properties of these enzymes...
  15. ncbi request reprint Immobilization of thermophilic enzymes in miniaturized flow reactors
    A M Hickey
    School of Biosciences, Henry Wellcome Building for Biocatalysis, University of Exeter, Stocker Road, Exeter EX4 4QD, U K
    Biochem Soc Trans 35:1621-3. 2007
    ..The enzyme has been successfully immobilized and used to produce stable biocatalytic microreactors that can be used repeatedly over a period of several months...
  16. ncbi request reprint The dodecameric vanadium-dependent haloperoxidase from the marine algae Corallina officinalis: cloning, expression, and refolding of the recombinant enzyme
    E E Coupe
    The Henry Wellcome Building for Biocatalysis, School of Biosciences, University of Exeter, Exeter EX4 4QD, UK
    Protein Expr Purif 52:265-72. 2007
    ..5-10 and in organic solvents such as ethanol, acetonitrile, methanol, and acetone. The novel refolding approach reported in this paper opens up the full potential of this versatile enzyme for use in large scale biotransformation studies...
  17. ncbi request reprint Substrate binding is required for assembly of the active conformation of the catalytic site in Ntn amidotransferases: evidence from the 1.8 A crystal structure of the glutaminase domain of glucosamine 6-phosphate synthase
    M N Isupov
    Department of Chemistry and Biological Sciences, University of Exeter, UK
    Structure 4:801-10. 1996
    ..To gain insight into the mechanism of nitrogen transfer, we examined the structure of the glutaminase domain of glucosamine 6-phosphate synthase (GLMS)...
  18. ncbi request reprint Enhancing effect of calcium and vanadium ions on thermal stability of bromoperoxidase from Corallina pilulifera
    Esther Garcia-Rodriguez
    Henry Wellcome Building for Biocatalysis, School of Biological and Chemical Sciences, University of Exeter, Exeter, EX4 4QD, UK
    J Biol Inorg Chem 10:275-82. 2005
    ..The high stability of the enzyme in the presence of organic solvents is useful for its application as a biocatalyst...
  19. ncbi request reprint Studies with inhibitors of the glycolytic enzyme phosphoglycerate kinase for potential treatment of cardiovascular and respiratory disorders
    J McHarg
    Department of Chemistry, University of Exeter, UK
    J Pharm Pharmacol 48:201-5. 1996
    ..These residues are shown to bind to the D-hydroxyl moiety on the C2 of the true substrate, 3-phosphoglycerate, in the high-resolution crystal structure of pig muscle PGK co-crystallized with 3-phosphoglycerate...
  20. pmc Modification of halogen specificity of a vanadium-dependent bromoperoxidase
    Takashi Ohshiro
    Department of Biotechnology, Tottori University, Japan
    Protein Sci 13:1566-71. 2004
    ..These results supported the existence of a specific halogen binding site within the catalytic cleft of vanadium haloperoxidases...