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Species | M LevasseurSummaryAffiliation: University of Newcastle Country: UK Publications
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Publications
Making cRNA for microinjection and expression of fluorescently tagged proteins for live-cell imaging in oocytesMark Levasseur
Institute for Cell and Molecular Biosciences, The Medical School, Newcastle University, Newcastle, UK
Methods Mol Biol 957:121-34. 2013..This chapter describes how to produce cRNA coding for a fluorescently tagged protein of choice, such that it is suitable for microinjection and subsequent expression studies in live oocytes...
A novel mechanism controls the Ca2+ oscillations triggered by activation of ascidian eggs and has an absolute requirement for Cdk1 activityMark Levasseur
Institute for Cell and Molecular Biosciences, The Medical School, University of Newcastle, Newcastle upon Tyne, UK
J Cell Sci 120:1763-71. 2007..These findings suggest a new link between this cell cycle kinase and the Ins(1,4,5)P(3) pathway...- Inositol 1,4,5-trisphosphate (IP3) responsiveness is regulated in a meiotic cell cycle dependent manner: implications for fertilization induced calcium signalingMark Levasseur
School of Cell and Molecular Biosciences, University of Newcastle, The Medical School, Framlington Place, Newcastle upon Tyne NE2 4HH UK
Cell Cycle 2:610-3. 2003..We conclude that CDK1 plays a role, but is not the only factor involved in controlling IP3 responsiveness during the meiotic cell cycle... - Sperm-induced calcium oscillations at fertilisation in ascidians are controlled by cyclin B1-dependent kinase activityM Levasseur
Department of Physiological Sciences, The Medical School, University of Newcastle upon Tyne, Framlington Place, Newcastle upon Tyne NE2 4HH, UK
Development 127:631-41. 2000..Furthermore, these findings may have a bearing upon the mitotic calcium signals of early embryos... - Cell cycle-dependent repetitive Ca(2+ )waves induced by a cytosolic sperm extract in mature ascidian eggs mimic those observed at fertilizationA McDougall
Department of Physiological Sciences, The Medical School, University of Newcastle upon Tyne, Framlington Place, Newcastle upon Tyne NE2 4HH, UK
J Cell Sci 113:3453-62. 2000..We demonstrated that ascidian sperm contain a protein factor(s) that is regulated by the egg CDK activity and can trigger all the Ca(2+ )waves observed at fertilization with a spatial pattern that mimics those initiated by sperm... - Ca(2+) oscillations promote APC/C-dependent cyclin B1 degradation during metaphase arrest and completion of meiosis in fertilizing mouse eggsVictoria L Nixon
Department of Physiological Sciences, The Medical School, Framlington Place, University of Newcastle, Newcastle NE2 4HH, United Kingdom
Curr Biol 12:746-50. 2002..Therefore, the physiological need for a repetitive Ca(2+) signal in mammals is to ensure long-term cyclin destruction during a protracted exit from meiosis... - Degradation of APCcdc20 and APCcdh1 substrates during the second meiotic division in mouse eggsHeng Yu Chang
Cell and Developmental Physiology Research Group, Institute for Cell and Molecular Biosciences, The Medical School, Framlington Place, University of Newcastle, Newcastle, NE2 4HH, UK
J Cell Sci 117:6289-96. 2004..Interestingly, therefore, we propose that mammalian eggs undergo meiosis II with both APCcdc20 and APCcdh1, whereas eggs of other species so far described have APCcdc20 activity only... - Exploring the mechanism of action of the sperm-triggered calcium-wave pacemaker in ascidian zygotesMichael Carroll
Cell and Developmental Physiology Group, School of Cell and Molecular Biosciences, The Medical School, University of Newcastle upon Tyne, Framlington Place, Newcastle upon Tyne NE2 4HH, UK
J Cell Sci 116:4997-5004. 2003..We suggest that the Ca2+-releasing sperm factor might be tethered near or on the PM and that following the cortical contraction, it is translocated to the vegetal CP, thus making that site act as a Ca2+-wave pacemaker... - Mad2 is required for inhibiting securin and cyclin B degradation following spindle depolymerisation in meiosis I mouse oocytesHayden A Homer
Newcastle Fertility Centre at Life, International Centre for Life, Times Square, Newcastle upon Tyne, NE1 4EP, UK
Reproduction 130:829-43. 2005..In conclusion, when all kinetochores lack attachment and tension, mouse oocytes mount a robust Mad2-dependent meiosis I arrest which inhibits the destruction of securin and cyclin B... - Sperm-triggered calcium oscillations during meiosis in ascidian oocytes first pause, restart, then stop: correlations with cell cycle kinase activityA McDougall
Department of Physiological Sciences, The Medical School, University of Newcastle, Framlington Place, Newcastle upon Tyne NE2 4HH, UK a
Development 125:4451-9. 1998..Finally we discuss our finding that the Ca2+ release system remains sensitive during the metaphase-like state (including the period when the Ca2+ oscillations pause)... 
The CRY box: a second APCcdh1-dependent degron in mammalian cdc20Alexandra Reis
Institute for Cell and Molecular Biosciences, The Medical School, University of Newcastle upon Tyne, Newcastle upon Tyne NE2 4HH, UK
EMBO Rep 7:1040-5. 2006..Thus, mammalian oocytes and embryos have the capacity to recognize two degrons in cdc20...- Prometaphase APCcdh1 activity prevents non-disjunction in mammalian oocytesAlexandra Reis
Institute for Cell and Molecular Biosciences, The Medical School, Framlington Place, University of Newcastle, Newcastle NE2 4HH, UK
Nat Cell Biol 9:1192-8. 2007..These findings demonstrate a fundamentally different mechanism of control for the first meiotic division in mammalian oocytes that is not observed in meioses of other species... - Calmodulin-dependent protein kinase II, and not protein kinase C, is sufficient for triggering cell-cycle resumption in mammalian eggsSuzanne Madgwick
Institute for Cell and Molecular Biosciences, The Medical School, Framlington Place, University of Newcastle, Newcastle, NE2 4HH, UK
J Cell Sci 118:3849-59. 2005..These data, combined with the knowledge that CamKII activity increase at fertilization, suggest that mouse eggs undergo cell-cycle resumption through stimulation of CamKII... - Mouse Emi2 is required to enter meiosis II by reestablishing cyclin B1 during interkinesisSuzanne Madgwick
Institute for Cell and Molecular Biosciences, The Medical School, University of Newcastle, Newcastle NE2 4HH, England, UK
J Cell Biol 174:791-801. 2006.... - Mad2 prevents aneuploidy and premature proteolysis of cyclin B and securin during meiosis I in mouse oocytesHayden A Homer
Newcastle Fertility Centre at Life, Centre for Life, Newcastle upon Tyne NE1 4EP, United Kingdom
Genes Dev 19:202-7. 2005..The data suggest a link between trisomies such as Down syndrome and defective oocyte spindle checkpoint function... - RNA interference in meiosis I human oocytes: towards an understanding of human aneuploidyHayden A Homer
Newcastle Fertility Centre at Life, International Centre for Life, Times Square, Newcastle upon Tyne NE1, UK
Mol Hum Reprod 11:397-404. 2005..By facilitating the investigation of candidate genes involved in regulating human female meiosis I, this approach can bring us closer to understanding the origins of aneuploidies such as Down's syndrome... - Restaging the spindle assembly checkpoint in female mammalian meiosis IHayden A Homer
Newcastle Fertility Centre at Life, Centre for Life, Times Square, Newcastle upon Tyne, UK
Cell Cycle 4:650-3. 2005..We discuss the implications of these results for the regulation of meiosis I in mammalian oocytes and for the genesis of human aneuploidy... - Ca(2+)-promoted cyclin B1 degradation in mouse oocytes requires the establishment of a metaphase arrestLouise A Hyslop
Cell and Developmental Physiology Research Group, School of Cell and Molecular Biosciences, The Medical School, University of Newcastle, Newcastle NE2 4HH, UK
Dev Biol 269:206-19. 2004..However, this stimulation occurs only in the presence of the ubiquitin ligase inhibitor CSF. We propose a model in which Ca2+ directly stimulates destruction of CSF during mammalian fertilisation... - APCcdh1 activity in mouse oocytes prevents entry into the first meiotic divisionAlexandra Reis
Institute for Cell and Molecular Biosciences, The Medical School, Framlington Place, University of Newcastle-upon-Tyne, Newcastle, NE2 4HH, UK
Nat Cell Biol 8:539-40. 2006..Here, we find that APCcdh1 is active in mouse oocytes and is necessary to maintain prophase arrest... - Maintenance of sister chromatid attachment in mouse eggs through maturation-promoting factor activitySuzanne Madgwick
Cell and Developmental Physiology Research Group, Institute of Cell and Molecular Biosciences, The Medical School, Framlington Place, University of Newcastle, NE2 4HH, UK
Dev Biol 275:68-81. 2004..We present a model in which metaphase II arrest is maintained primarily by MPF levels only... - Securin and not CDK1/cyclin B1 regulates sister chromatid disjunction during meiosis II in mouse eggsIbtissem Nabti
Institute for Cell and Molecular Biosciences, The Medical School, Framlington Place, University of Newcastle, Newcastle, NE2 4HH, UK
Dev Biol 321:379-86. 2008..In contrast, securin morpholino knockdown specifically induced loss of sister attachment, that could be restored by securin cRNA rescue. During metII arrest separase appears primarily regulated by securin binding, not CDK1/cyclin B1... - Homologue disjunction in mouse oocytes requires proteolysis of securin and cyclin B1Mary Herbert
Cell and Developmental Physiology Research Group, School of Surgical and Reproductive Sciences, Bioscience Centre, International Centre for Life, Times Square, Newcastle upon Tyne, NE1 4EP, UK
Nat Cell Biol 5:1023-5. 2003..These data indicate that the mechanisms regulating homologue disjunction in mammalian oocytes are similar to those of budding yeast and C.elegans...