M Levasseur

Summary

Affiliation: University of Newcastle
Country: UK

Publications

  1. doi request reprint Making cRNA for microinjection and expression of fluorescently tagged proteins for live-cell imaging in oocytes
    Mark Levasseur
    Institute for Cell and Molecular Biosciences, The Medical School, Newcastle University, Newcastle, UK
    Methods Mol Biol 957:121-34. 2013
  2. ncbi request reprint A novel mechanism controls the Ca2+ oscillations triggered by activation of ascidian eggs and has an absolute requirement for Cdk1 activity
    Mark Levasseur
    Institute for Cell and Molecular Biosciences, The Medical School, University of Newcastle, Newcastle upon Tyne, UK
    J Cell Sci 120:1763-71. 2007
  3. ncbi request reprint Inositol 1,4,5-trisphosphate (IP3) responsiveness is regulated in a meiotic cell cycle dependent manner: implications for fertilization induced calcium signaling
    Mark Levasseur
    School of Cell and Molecular Biosciences, University of Newcastle, The Medical School, Framlington Place, Newcastle upon Tyne NE2 4HH UK
    Cell Cycle 2:610-3. 2003
  4. ncbi request reprint Sperm-induced calcium oscillations at fertilisation in ascidians are controlled by cyclin B1-dependent kinase activity
    M Levasseur
    Department of Physiological Sciences, The Medical School, University of Newcastle upon Tyne, Framlington Place, Newcastle upon Tyne NE2 4HH, UK
    Development 127:631-41. 2000
  5. ncbi request reprint Cell cycle-dependent repetitive Ca(2+ )waves induced by a cytosolic sperm extract in mature ascidian eggs mimic those observed at fertilization
    A McDougall
    Department of Physiological Sciences, The Medical School, University of Newcastle upon Tyne, Framlington Place, Newcastle upon Tyne NE2 4HH, UK
    J Cell Sci 113:3453-62. 2000
  6. ncbi request reprint Ca(2+) oscillations promote APC/C-dependent cyclin B1 degradation during metaphase arrest and completion of meiosis in fertilizing mouse eggs
    Victoria L Nixon
    Department of Physiological Sciences, The Medical School, Framlington Place, University of Newcastle, Newcastle NE2 4HH, United Kingdom
    Curr Biol 12:746-50. 2002
  7. ncbi request reprint Degradation of APCcdc20 and APCcdh1 substrates during the second meiotic division in mouse eggs
    Heng Yu Chang
    Cell and Developmental Physiology Research Group, Institute for Cell and Molecular Biosciences, The Medical School, Framlington Place, University of Newcastle, Newcastle, NE2 4HH, UK
    J Cell Sci 117:6289-96. 2004
  8. ncbi request reprint Exploring the mechanism of action of the sperm-triggered calcium-wave pacemaker in ascidian zygotes
    Michael Carroll
    Cell and Developmental Physiology Group, School of Cell and Molecular Biosciences, The Medical School, University of Newcastle upon Tyne, Framlington Place, Newcastle upon Tyne NE2 4HH, UK
    J Cell Sci 116:4997-5004. 2003
  9. ncbi request reprint Mad2 is required for inhibiting securin and cyclin B degradation following spindle depolymerisation in meiosis I mouse oocytes
    Hayden A Homer
    Newcastle Fertility Centre at Life, International Centre for Life, Times Square, Newcastle upon Tyne, NE1 4EP, UK
    Reproduction 130:829-43. 2005
  10. ncbi request reprint Sperm-triggered calcium oscillations during meiosis in ascidian oocytes first pause, restart, then stop: correlations with cell cycle kinase activity
    A McDougall
    Department of Physiological Sciences, The Medical School, University of Newcastle, Framlington Place, Newcastle upon Tyne NE2 4HH, UK a
    Development 125:4451-9. 1998

Collaborators

Detail Information

Publications22

  1. doi request reprint Making cRNA for microinjection and expression of fluorescently tagged proteins for live-cell imaging in oocytes
    Mark Levasseur
    Institute for Cell and Molecular Biosciences, The Medical School, Newcastle University, Newcastle, UK
    Methods Mol Biol 957:121-34. 2013
    ..This chapter describes how to produce cRNA coding for a fluorescently tagged protein of choice, such that it is suitable for microinjection and subsequent expression studies in live oocytes...
  2. ncbi request reprint A novel mechanism controls the Ca2+ oscillations triggered by activation of ascidian eggs and has an absolute requirement for Cdk1 activity
    Mark Levasseur
    Institute for Cell and Molecular Biosciences, The Medical School, University of Newcastle, Newcastle upon Tyne, UK
    J Cell Sci 120:1763-71. 2007
    ..These findings suggest a new link between this cell cycle kinase and the Ins(1,4,5)P(3) pathway...
  3. ncbi request reprint Inositol 1,4,5-trisphosphate (IP3) responsiveness is regulated in a meiotic cell cycle dependent manner: implications for fertilization induced calcium signaling
    Mark Levasseur
    School of Cell and Molecular Biosciences, University of Newcastle, The Medical School, Framlington Place, Newcastle upon Tyne NE2 4HH UK
    Cell Cycle 2:610-3. 2003
    ..We conclude that CDK1 plays a role, but is not the only factor involved in controlling IP3 responsiveness during the meiotic cell cycle...
  4. ncbi request reprint Sperm-induced calcium oscillations at fertilisation in ascidians are controlled by cyclin B1-dependent kinase activity
    M Levasseur
    Department of Physiological Sciences, The Medical School, University of Newcastle upon Tyne, Framlington Place, Newcastle upon Tyne NE2 4HH, UK
    Development 127:631-41. 2000
    ..Furthermore, these findings may have a bearing upon the mitotic calcium signals of early embryos...
  5. ncbi request reprint Cell cycle-dependent repetitive Ca(2+ )waves induced by a cytosolic sperm extract in mature ascidian eggs mimic those observed at fertilization
    A McDougall
    Department of Physiological Sciences, The Medical School, University of Newcastle upon Tyne, Framlington Place, Newcastle upon Tyne NE2 4HH, UK
    J Cell Sci 113:3453-62. 2000
    ..We demonstrated that ascidian sperm contain a protein factor(s) that is regulated by the egg CDK activity and can trigger all the Ca(2+ )waves observed at fertilization with a spatial pattern that mimics those initiated by sperm...
  6. ncbi request reprint Ca(2+) oscillations promote APC/C-dependent cyclin B1 degradation during metaphase arrest and completion of meiosis in fertilizing mouse eggs
    Victoria L Nixon
    Department of Physiological Sciences, The Medical School, Framlington Place, University of Newcastle, Newcastle NE2 4HH, United Kingdom
    Curr Biol 12:746-50. 2002
    ..Therefore, the physiological need for a repetitive Ca(2+) signal in mammals is to ensure long-term cyclin destruction during a protracted exit from meiosis...
  7. ncbi request reprint Degradation of APCcdc20 and APCcdh1 substrates during the second meiotic division in mouse eggs
    Heng Yu Chang
    Cell and Developmental Physiology Research Group, Institute for Cell and Molecular Biosciences, The Medical School, Framlington Place, University of Newcastle, Newcastle, NE2 4HH, UK
    J Cell Sci 117:6289-96. 2004
    ..Interestingly, therefore, we propose that mammalian eggs undergo meiosis II with both APCcdc20 and APCcdh1, whereas eggs of other species so far described have APCcdc20 activity only...
  8. ncbi request reprint Exploring the mechanism of action of the sperm-triggered calcium-wave pacemaker in ascidian zygotes
    Michael Carroll
    Cell and Developmental Physiology Group, School of Cell and Molecular Biosciences, The Medical School, University of Newcastle upon Tyne, Framlington Place, Newcastle upon Tyne NE2 4HH, UK
    J Cell Sci 116:4997-5004. 2003
    ..We suggest that the Ca2+-releasing sperm factor might be tethered near or on the PM and that following the cortical contraction, it is translocated to the vegetal CP, thus making that site act as a Ca2+-wave pacemaker...
  9. ncbi request reprint Mad2 is required for inhibiting securin and cyclin B degradation following spindle depolymerisation in meiosis I mouse oocytes
    Hayden A Homer
    Newcastle Fertility Centre at Life, International Centre for Life, Times Square, Newcastle upon Tyne, NE1 4EP, UK
    Reproduction 130:829-43. 2005
    ..In conclusion, when all kinetochores lack attachment and tension, mouse oocytes mount a robust Mad2-dependent meiosis I arrest which inhibits the destruction of securin and cyclin B...
  10. ncbi request reprint Sperm-triggered calcium oscillations during meiosis in ascidian oocytes first pause, restart, then stop: correlations with cell cycle kinase activity
    A McDougall
    Department of Physiological Sciences, The Medical School, University of Newcastle, Framlington Place, Newcastle upon Tyne NE2 4HH, UK a
    Development 125:4451-9. 1998
    ..Finally we discuss our finding that the Ca2+ release system remains sensitive during the metaphase-like state (including the period when the Ca2+ oscillations pause)...
  11. pmc The CRY box: a second APCcdh1-dependent degron in mammalian cdc20
    Alexandra Reis
    Institute for Cell and Molecular Biosciences, The Medical School, University of Newcastle upon Tyne, Newcastle upon Tyne NE2 4HH, UK
    EMBO Rep 7:1040-5. 2006
    ..Thus, mammalian oocytes and embryos have the capacity to recognize two degrons in cdc20...
  12. pmc Prometaphase APCcdh1 activity prevents non-disjunction in mammalian oocytes
    Alexandra Reis
    Institute for Cell and Molecular Biosciences, The Medical School, Framlington Place, University of Newcastle, Newcastle NE2 4HH, UK
    Nat Cell Biol 9:1192-8. 2007
    ..These findings demonstrate a fundamentally different mechanism of control for the first meiotic division in mammalian oocytes that is not observed in meioses of other species...
  13. ncbi request reprint Calmodulin-dependent protein kinase II, and not protein kinase C, is sufficient for triggering cell-cycle resumption in mammalian eggs
    Suzanne Madgwick
    Institute for Cell and Molecular Biosciences, The Medical School, Framlington Place, University of Newcastle, Newcastle, NE2 4HH, UK
    J Cell Sci 118:3849-59. 2005
    ..These data, combined with the knowledge that CamKII activity increase at fertilization, suggest that mouse eggs undergo cell-cycle resumption through stimulation of CamKII...
  14. pmc Mouse Emi2 is required to enter meiosis II by reestablishing cyclin B1 during interkinesis
    Suzanne Madgwick
    Institute for Cell and Molecular Biosciences, The Medical School, University of Newcastle, Newcastle NE2 4HH, England, UK
    J Cell Biol 174:791-801. 2006
    ....
  15. pmc Mad2 prevents aneuploidy and premature proteolysis of cyclin B and securin during meiosis I in mouse oocytes
    Hayden A Homer
    Newcastle Fertility Centre at Life, Centre for Life, Newcastle upon Tyne NE1 4EP, United Kingdom
    Genes Dev 19:202-7. 2005
    ..The data suggest a link between trisomies such as Down syndrome and defective oocyte spindle checkpoint function...
  16. ncbi request reprint RNA interference in meiosis I human oocytes: towards an understanding of human aneuploidy
    Hayden A Homer
    Newcastle Fertility Centre at Life, International Centre for Life, Times Square, Newcastle upon Tyne NE1, UK
    Mol Hum Reprod 11:397-404. 2005
    ..By facilitating the investigation of candidate genes involved in regulating human female meiosis I, this approach can bring us closer to understanding the origins of aneuploidies such as Down's syndrome...
  17. ncbi request reprint Restaging the spindle assembly checkpoint in female mammalian meiosis I
    Hayden A Homer
    Newcastle Fertility Centre at Life, Centre for Life, Times Square, Newcastle upon Tyne, UK
    Cell Cycle 4:650-3. 2005
    ..We discuss the implications of these results for the regulation of meiosis I in mammalian oocytes and for the genesis of human aneuploidy...
  18. ncbi request reprint Ca(2+)-promoted cyclin B1 degradation in mouse oocytes requires the establishment of a metaphase arrest
    Louise A Hyslop
    Cell and Developmental Physiology Research Group, School of Cell and Molecular Biosciences, The Medical School, University of Newcastle, Newcastle NE2 4HH, UK
    Dev Biol 269:206-19. 2004
    ..However, this stimulation occurs only in the presence of the ubiquitin ligase inhibitor CSF. We propose a model in which Ca2+ directly stimulates destruction of CSF during mammalian fertilisation...
  19. pmc APCcdh1 activity in mouse oocytes prevents entry into the first meiotic division
    Alexandra Reis
    Institute for Cell and Molecular Biosciences, The Medical School, Framlington Place, University of Newcastle upon Tyne, Newcastle, NE2 4HH, UK
    Nat Cell Biol 8:539-40. 2006
    ..Here, we find that APCcdh1 is active in mouse oocytes and is necessary to maintain prophase arrest...
  20. ncbi request reprint Maintenance of sister chromatid attachment in mouse eggs through maturation-promoting factor activity
    Suzanne Madgwick
    Cell and Developmental Physiology Research Group, Institute of Cell and Molecular Biosciences, The Medical School, Framlington Place, University of Newcastle, NE2 4HH, UK
    Dev Biol 275:68-81. 2004
    ..We present a model in which metaphase II arrest is maintained primarily by MPF levels only...
  21. doi request reprint Securin and not CDK1/cyclin B1 regulates sister chromatid disjunction during meiosis II in mouse eggs
    Ibtissem Nabti
    Institute for Cell and Molecular Biosciences, The Medical School, Framlington Place, University of Newcastle, Newcastle, NE2 4HH, UK
    Dev Biol 321:379-86. 2008
    ..In contrast, securin morpholino knockdown specifically induced loss of sister attachment, that could be restored by securin cRNA rescue. During metII arrest separase appears primarily regulated by securin binding, not CDK1/cyclin B1...
  22. ncbi request reprint Homologue disjunction in mouse oocytes requires proteolysis of securin and cyclin B1
    Mary Herbert
    Cell and Developmental Physiology Research Group, School of Surgical and Reproductive Sciences, Bioscience Centre, International Centre for Life, Times Square, Newcastle upon Tyne, NE1 4EP, UK
    Nat Cell Biol 5:1023-5. 2003
    ..These data indicate that the mechanisms regulating homologue disjunction in mammalian oocytes are similar to those of budding yeast and C.elegans...