Meng Huee Lee

Summary

Affiliation: University of East Anglia
Country: UK

Publications

  1. ncbi Identification of the extracellular matrix (ECM) binding motifs of tissue inhibitor of metalloproteinases (TIMP)-3 and effective transfer to TIMP-1
    Meng Huee Lee
    Department of Oncology, Cancer Research UK Cambridge Research Institute, Cambridge University, Cambridge CB2 0RE, United Kingdom
    J Biol Chem 282:6887-98. 2007
  2. pmc Engineering N-terminal domain of tissue inhibitor of metalloproteinase (TIMP)-3 to be a better inhibitor against tumour necrosis factor-alpha-converting enzyme
    Meng Huee Lee
    School of Biological Sciences, University of East Anglia, Norwich NR4 7TJ, U K
    Biochem J 364:227-34. 2002
  3. ncbi The C-terminal domains of TACE weaken the inhibitory action of N-TIMP-3
    Meng Huee Lee
    School of Biological Sciences, University of East Anglia, Norwich NR4 7TJ, UK
    FEBS Lett 520:102-6. 2002
  4. pmc Tailoring tissue inhibitor of metalloproteinases-3 to overcome the weakening effects of the cysteine-rich domains of tumour necrosis factor-alpha converting enzyme
    Meng Huee Lee
    School of Biological Sciences, University of East Anglia, Norwich, NR4 7TJ, UK
    Biochem J 371:369-76. 2003
  5. pmc Mapping and characterization of the functional epitopes of tissue inhibitor of metalloproteinases (TIMP)-3 using TIMP-1 as the scaffold: a new frontier in TIMP engineering
    Meng Huee Lee
    School of Biological Sciences, University of East Anglia, Norwich NR4 7TJ, UK
    Protein Sci 11:2493-503. 2002
  6. ncbi Total conversion of tissue inhibitor of metalloproteinase (TIMP) for specific metalloproteinase targeting: fine-tuning TIMP-4 for optimal inhibition of tumor necrosis factor-{alpha}-converting enzyme
    Meng Huee Lee
    Department of Oncology, Cambridge University, Cambridge Institute for Medical Research, Wellcome Trust MRC Building, Cambridge CB2 2XY, United Kingdom
    J Biol Chem 280:15967-75. 2005
  7. ncbi Delineating the molecular basis of the inactivity of tissue inhibitor of metalloproteinase-2 against tumor necrosis factor-alpha-converting enzyme
    Meng Huee Lee
    Department of Oncology, Cambridge University, Cambridge Institute for Medical Research, Wellcome Trust Medical Research Council Building, Cambridge CB2 2XY, United Kingdom
    J Biol Chem 279:45121-9. 2004
  8. ncbi Threonine 98, the pivotal residue of tissue inhibitor of metalloproteinases (TIMP)-1 in metalloproteinase recognition
    Meng Huee Lee
    Cambridge Institute for Medical Research, Wellcome Trust Medical Research Council Building, Cambridge University, Cambridge CB2 2XY, United Kingdom
    J Biol Chem 279:17562-9. 2004
  9. ncbi Unveiling the surface epitopes that render tissue inhibitor of metalloproteinase-1 inactive against membrane type 1-matrix metalloproteinase
    Meng Huee Lee
    Cambridge Institute for Medical Research, Wellcome Trust MRC Building, Cambridge University, Cambridge CB2 2XY, United Kingdom
    J Biol Chem 278:40224-30. 2003
  10. ncbi Matrix metalloproteinases at a glance
    Meng Huee Lee
    Dept of Oncology, University of Cambridge, Cambridge Institute for Medical Research, Hills Road, Cambridge CB2 2XY, UK
    J Cell Sci 117:4015-6. 2004

Collaborators

Detail Information

Publications11

  1. ncbi Identification of the extracellular matrix (ECM) binding motifs of tissue inhibitor of metalloproteinases (TIMP)-3 and effective transfer to TIMP-1
    Meng Huee Lee
    Department of Oncology, Cancer Research UK Cambridge Research Institute, Cambridge University, Cambridge CB2 0RE, United Kingdom
    J Biol Chem 282:6887-98. 2007
    ..This is the first instance of TIMPs being intentionally rendered soluble or ECM-bound. The ability to prepare TIMPs in soluble or ECM-bound forms also opens new avenues for future TIMP research...
  2. pmc Engineering N-terminal domain of tissue inhibitor of metalloproteinase (TIMP)-3 to be a better inhibitor against tumour necrosis factor-alpha-converting enzyme
    Meng Huee Lee
    School of Biological Sciences, University of East Anglia, Norwich NR4 7TJ, U K
    Biochem J 364:227-34. 2002
    ....
  3. ncbi The C-terminal domains of TACE weaken the inhibitory action of N-TIMP-3
    Meng Huee Lee
    School of Biological Sciences, University of East Anglia, Norwich NR4 7TJ, UK
    FEBS Lett 520:102-6. 2002
    ..Our findings highlight the potential role of the C-terminal domains of ADAM proteinases in influencing TIMP interactions...
  4. pmc Tailoring tissue inhibitor of metalloproteinases-3 to overcome the weakening effects of the cysteine-rich domains of tumour necrosis factor-alpha converting enzyme
    Meng Huee Lee
    School of Biological Sciences, University of East Anglia, Norwich, NR4 7TJ, UK
    Biochem J 371:369-76. 2003
    ..Further expression of one of the mutants, Lys(26/27/30/76)-->Glu, in a mammalian expression system confirmed that TIMP-3 associates with the extracellular matrix via its C-terminal domain...
  5. pmc Mapping and characterization of the functional epitopes of tissue inhibitor of metalloproteinases (TIMP)-3 using TIMP-1 as the scaffold: a new frontier in TIMP engineering
    Meng Huee Lee
    School of Biological Sciences, University of East Anglia, Norwich NR4 7TJ, UK
    Protein Sci 11:2493-503. 2002
    ..Most importantly, our findings confirm that the individual characteristics of TIMP could be transplanted from one variant to another...
  6. ncbi Total conversion of tissue inhibitor of metalloproteinase (TIMP) for specific metalloproteinase targeting: fine-tuning TIMP-4 for optimal inhibition of tumor necrosis factor-{alpha}-converting enzyme
    Meng Huee Lee
    Department of Oncology, Cambridge University, Cambridge Institute for Medical Research, Wellcome Trust MRC Building, Cambridge CB2 2XY, United Kingdom
    J Biol Chem 280:15967-75. 2005
    ..Drawing together our previous experience in TACE-targeted mutagenesis by using TIMP-1 and -2 scaffolds, we have finally resolved the mystery of the selective sensitivity of TACE to TIMP-3...
  7. ncbi Delineating the molecular basis of the inactivity of tissue inhibitor of metalloproteinase-2 against tumor necrosis factor-alpha-converting enzyme
    Meng Huee Lee
    Department of Oncology, Cambridge University, Cambridge Institute for Medical Research, Wellcome Trust Medical Research Council Building, Cambridge CB2 2XY, United Kingdom
    J Biol Chem 279:45121-9. 2004
    ..Our findings here represent a significant advance toward designing tailor-made TIMPs for specific MP targeting...
  8. ncbi Threonine 98, the pivotal residue of tissue inhibitor of metalloproteinases (TIMP)-1 in metalloproteinase recognition
    Meng Huee Lee
    Cambridge Institute for Medical Research, Wellcome Trust Medical Research Council Building, Cambridge University, Cambridge CB2 2XY, United Kingdom
    J Biol Chem 279:17562-9. 2004
    ..Not only have we unlocked the molecular basis for the inactivity of TIMP-1 against several of the MPs, but also our findings fundamentally modify the current beliefs on the molecular mechanism of TIMP-MP recognition and selectivity...
  9. ncbi Unveiling the surface epitopes that render tissue inhibitor of metalloproteinase-1 inactive against membrane type 1-matrix metalloproteinase
    Meng Huee Lee
    Cambridge Institute for Medical Research, Wellcome Trust MRC Building, Cambridge University, Cambridge CB2 2XY, United Kingdom
    J Biol Chem 278:40224-30. 2003
    ..Our findings suggest that threonine 98 is critical in initiating MMP binding and complex stabilization. Our findings also provide a potential mechanistic explanation for MMP-TIMP selectivity...
  10. ncbi Matrix metalloproteinases at a glance
    Meng Huee Lee
    Dept of Oncology, University of Cambridge, Cambridge Institute for Medical Research, Hills Road, Cambridge CB2 2XY, UK
    J Cell Sci 117:4015-6. 2004
  11. doi The isolated N-terminal domains of TIMP-1 and TIMP-3 are insufficient for ADAM10 inhibition
    Magdalini Rapti
    Department of Oncology, Cambridge University, Cancer Research UK Cambridge Research Institute, Li Ka Shing Centre, Cambridge CB2 0RE, UK
    Biochem J 411:433-9. 2008
    ..This knowledge could form the basis for the design of directed inhibitors against different metalloproteinases...