G E Lash

Summary

Affiliation: University of Newcastle
Country: UK

Publications

  1. doi request reprint Do uterine natural killer (uNK) cells contribute to female reproductive disorders?
    Gendie E Lash
    Reproductive and Vascular Biology Group, Institute of Cellular Medicine, Newcastle University, Newcastle upon Tyne NE2 4HH, United Kingdom
    J Reprod Immunol 88:156-64. 2011
  2. ncbi request reprint The effects of angiogenic growth factors on extravillous trophoblast invasion and motility
    G E Lash
    School of Human Development, Department of Obstetrics and Gynaecology, University of Nottingham, City Hospital, Hucknall Road, Nottingham, NG5 1PB, UK
    Placenta 20:661-7. 1999
  3. ncbi request reprint Effect of low oxygen concentrations on trophoblast-like cell line invasion
    G E Lash
    School of Surgical and Reproductive Sciences, 3rd Floor, William Leech Building, University of Newcastle upon Tyne, Newcastle upon Tyne NE2 4HH, UK
    Placenta 28:390-8. 2007
  4. ncbi request reprint Gestational diseases -- a workshop report
    G E Lash
    Uterine Cell Signalling Group, Institute of Cellular Medicine, 3rd Floor, William Leech Building, Newcastle University, Newcastle upon Tyne NE2 4HH, UK
    Placenta 29:S92-4. 2008
  5. doi request reprint The role of vascular smooth muscle cell apoptosis and migration during uterine spiral artery remodeling in normal human pregnancy
    Judith N Bulmer
    Reproductive and Vascular Biology Group, Institute of Cellular Medicine, Newcastle University, Newcastle upon Tyne, UK
    FASEB J 26:2975-85. 2012
  6. ncbi request reprint Low oxygen concentrations inhibit trophoblast cell invasion from early gestation placental explants via alterations in levels of the urokinase plasminogen activator system
    Gendie E Lash
    Schools of Surgical and Reproductive Sciences, University of Newcastle upon Tyne, Newcastle upon Tyne, NE2 4HH, United Kingdom
    Biol Reprod 74:403-9. 2006
  7. ncbi request reprint Comparison of three multiplex cytokine analysis systems: Luminex, SearchLight and FAST Quant
    Gendie E Lash
    School of Surgical and Reproductive Sciences, 3rd Floor, William Leech Building, University of Newcastle upon Tyne, Newcastle upon Tyne, NE2 4HH, UK
    J Immunol Methods 309:205-8. 2006
  8. ncbi request reprint Expression of angiogenic growth factors by uterine natural killer cells during early pregnancy
    Gendie E Lash
    School of Surgical and Reproductive Sciences, 3rd Floor, William Leech Building, University of Newcastle upon Tyne, Newcastle upon Tyne, NE2 4HH, UK
    J Leukoc Biol 80:572-80. 2006
  9. ncbi request reprint Interferon-gamma inhibits extravillous trophoblast cell invasion by a mechanism that involves both changes in apoptosis and protease levels
    Gendie E Lash
    School of Surgical and Reproductive Sciences, 3rd Floor, William Leech Bldg, University of Newcastle upon Tyne, Newcastle upon Tyne NE2 4HH, UK
    FASEB J 20:2512-8. 2006
  10. doi request reprint IFPA Meeting 2009 workshops report
    G E Lash
    Institute of Cellular Medicine, Newcastle University, 3rd Floor, William Leech Building, Newcastle upon Tyne NE2 4HH, UK
    Placenta 31:S4-20. 2010

Detail Information

Publications38

  1. doi request reprint Do uterine natural killer (uNK) cells contribute to female reproductive disorders?
    Gendie E Lash
    Reproductive and Vascular Biology Group, Institute of Cellular Medicine, Newcastle University, Newcastle upon Tyne NE2 4HH, United Kingdom
    J Reprod Immunol 88:156-64. 2011
    ..Understanding of the function of uNK cells in non-pregnant and pregnant endometrium is now increasing; the potential contribution of altered numbers and function of uNK cells to reproductive disorders is the focus of this review...
  2. ncbi request reprint The effects of angiogenic growth factors on extravillous trophoblast invasion and motility
    G E Lash
    School of Human Development, Department of Obstetrics and Gynaecology, University of Nottingham, City Hospital, Hucknall Road, Nottingham, NG5 1PB, UK
    Placenta 20:661-7. 1999
    ....
  3. ncbi request reprint Effect of low oxygen concentrations on trophoblast-like cell line invasion
    G E Lash
    School of Surgical and Reproductive Sciences, 3rd Floor, William Leech Building, University of Newcastle upon Tyne, Newcastle upon Tyne NE2 4HH, UK
    Placenta 28:390-8. 2007
    ..Care should be taken before results obtained using cell lines as a model for EVT are extrapolated to extravillous trophoblast cell behaviour in vivo...
  4. ncbi request reprint Gestational diseases -- a workshop report
    G E Lash
    Uterine Cell Signalling Group, Institute of Cellular Medicine, 3rd Floor, William Leech Building, Newcastle University, Newcastle upon Tyne NE2 4HH, UK
    Placenta 29:S92-4. 2008
    ..Both complete and partial hydatidiform mole are associated with excessive trophoblast proliferation and hence provide an opportunity to study trophoblast growth regulation...
  5. doi request reprint The role of vascular smooth muscle cell apoptosis and migration during uterine spiral artery remodeling in normal human pregnancy
    Judith N Bulmer
    Reproductive and Vascular Biology Group, Institute of Cellular Medicine, Newcastle University, Newcastle upon Tyne, UK
    FASEB J 26:2975-85. 2012
    ..In summary, apoptosis is unlikely to play a major role in loss of VSMCs from SpAs during remodeling in normal pregnancy, but VSMCs appear to migrate away from the wall of the SpA, an effect enhanced by the presence of EVT cells...
  6. ncbi request reprint Low oxygen concentrations inhibit trophoblast cell invasion from early gestation placental explants via alterations in levels of the urokinase plasminogen activator system
    Gendie E Lash
    Schools of Surgical and Reproductive Sciences, University of Newcastle upon Tyne, Newcastle upon Tyne, NE2 4HH, United Kingdom
    Biol Reprod 74:403-9. 2006
    ..The reduction in the invasive capacity of EVT cultured at 3% oxygen appears to be mediated both by a general inhibition of the PLAU system and a decrease in the number of cells available to invade...
  7. ncbi request reprint Comparison of three multiplex cytokine analysis systems: Luminex, SearchLight and FAST Quant
    Gendie E Lash
    School of Surgical and Reproductive Sciences, 3rd Floor, William Leech Building, University of Newcastle upon Tyne, Newcastle upon Tyne, NE2 4HH, UK
    J Immunol Methods 309:205-8. 2006
    ..We also suggest that results obtained from different systems cannot be combined...
  8. ncbi request reprint Expression of angiogenic growth factors by uterine natural killer cells during early pregnancy
    Gendie E Lash
    School of Surgical and Reproductive Sciences, 3rd Floor, William Leech Building, University of Newcastle upon Tyne, Newcastle upon Tyne, NE2 4HH, UK
    J Leukoc Biol 80:572-80. 2006
    ..uNK cells appear to be a prominent source of Ang1, Ang2, TGF-beta1, and VEGF-C within the placental bed...
  9. ncbi request reprint Interferon-gamma inhibits extravillous trophoblast cell invasion by a mechanism that involves both changes in apoptosis and protease levels
    Gendie E Lash
    School of Surgical and Reproductive Sciences, 3rd Floor, William Leech Bldg, University of Newcastle upon Tyne, Newcastle upon Tyne NE2 4HH, UK
    FASEB J 20:2512-8. 2006
    ..Many decidual factors are likely to play a role in regulating this process, including uterine natural killer (uNK) cell-derived cytokines...
  10. doi request reprint IFPA Meeting 2009 workshops report
    G E Lash
    Institute of Cellular Medicine, Newcastle University, 3rd Floor, William Leech Building, Newcastle upon Tyne NE2 4HH, UK
    Placenta 31:S4-20. 2010
    ..This report is a full summary of the various topics covered...
  11. doi request reprint Review: Functional role of uterine natural killer (uNK) cells in human early pregnancy decidua
    G E Lash
    Institute of Cellular Medicine, Newcastle University, Newcastle upon Tyne NE2 4HH, United Kingdom
    Placenta 31:S87-92. 2010
    ..Herein we review the functional roles of this important decidual cell type...
  12. pmc IFPA meeting 2008 workshops report
    G E Lash
    Institute of Cellular Medicine, Newcastle University, Newcastle upon Tyne, UK
    Placenta 30:S4-14. 2009
    ..This report is a summary of the various topics covered...
  13. doi request reprint Regulation of extravillous trophoblast invasion by uterine natural killer cells is dependent on gestational age
    Gendie E Lash
    Uterine Cell Signalling Group, Institute of Cellular Medicine and School of Medical Development and Education, Newcastle University, 3rd Floor, William Leech Building, Newcastle upon Tyne NE2 4HH, UK
    Hum Reprod 25:1137-45. 2010
    ..We therefore hypothesized that supernatants from purified uNK cells would inhibit EVT invasion...
  14. ncbi request reprint Inhibition of trophoblast cell invasion by TGFB1, 2, and 3 is associated with a decrease in active proteases
    Gendie E Lash
    Schools of Surgical and Reproductive Sciences, University of Newcastle upon Tyne, Newcastle Tyne NE2 4HH, United Kingdom
    Biol Reprod 73:374-81. 2005
    ..MMP2 and tissue inhibitor of metalloproteinase 1 and 3 levels were not altered. These results suggest that TGFB1, 2, and 3 inhibit trophoblast invasion by a mechanism dependent on reduced protease activity...
  15. doi request reprint Secretion of angiogenic growth factors by villous cytotrophoblast and extravillous trophoblast in early human pregnancy
    G E Lash
    Reproductive and Vascular Biology Group, Institute of Cellular Medicine and School of Medical Development and Education, Newcastle University, Newcastle upon Tyne, UK
    Placenta 31:545-8. 2010
    ..Gestational age and cell type differences were observed. EVT and CTB are major producers of angiogenic growth factors that likely contribute to placental vascular development and spiral artery remodeling...
  16. doi request reprint Localization of angiogenic growth factors and their receptors in the human endometrium throughout the menstrual cycle and in recurrent miscarriage
    Gendie E Lash
    Reproductive and Vascular Biology Group, Institute of Cellular Medicine, Newcastle University, 3rd Floor, William Leech Building, Newcastle upon Tyne NE2 4HH, UK
    Hum Reprod 27:183-95. 2012
    ..Angiogenesis is a key feature of endometrial development. Inappropriate endometrial vascular development has been associated with recurrent miscarriage (RM) with increased amounts of perivascular smooth muscle cells surrounding them...
  17. doi request reprint Prednisolone treatment reduces endometrial spiral artery development in women with recurrent miscarriage
    Gendie E Lash
    Reproductive and Vascular Biology Group, Institute of Cellular Medicine, Newcastle University, 3rd Floor, William Leech Building, Newcastle upon Tyne, NE2 4HH, UK
    Angiogenesis 14:523-32. 2011
    ..uNK cell numbers are reduced after treatment with prednisolone (20 mg/day for 3 weeks)...
  18. doi request reprint Interaction between uterine natural killer cells and extravillous trophoblast cells: effect on cytokine and angiogenic growth factor production
    Gendie E Lash
    Reproductive and Vascular Biology Group, Institute of Cellular Medicine, Newcastle University, Newcastle upon Tyne NE2 4HH UK
    Hum Reprod 26:2289-95. 2011
    ..We hypothesize that uNK cell interaction with EVT cells alters their cytokine and AGF secretion...
  19. ncbi request reprint Vascular endothelial growth factor and placental growth factor release in cultured trophoblast cells under different oxygen tensions
    G E Lash
    Department of Anatomy and Cell Biology 9th Floor, Botterell Hall, Queen s University Kingston, Ont, K7L 3N6, Canada
    Growth Factors 20:189-96. 2002
    ..These altered levels of secreted VEGF andPIGF may be released as compensatory molecules in the pathogenesis of diseases such as pre-eclampsia and intrauterine growth restriction...
  20. ncbi request reprint Vascular endothelial growth factor is a chemoattractant for trophoblast cells
    G E Lash
    School of Human Development, City Hospital, Hucknall Road, Nottingham, NG5 1PB, UK
    Placenta 24:549-56. 2003
    ..In conclusion, the first trimester and term trophoblast cells appeared to respond differently to the various factors tested in the present study that may reflect differential cellular function as gestation progresses...
  21. doi request reprint Multiplex cytokine analysis technologies
    Gendie E Lash
    Reproductive and Vascular Biology Group, Institute of Cellular Medicine, Newcastle University, Newcastle upon Tyne, NE2 4HH, UK
    Expert Rev Vaccines 9:1231-7. 2010
    ..This article examines some of the different mRNA and protein multiplex platforms available and how they may be used in assessing vaccine immunity...
  22. doi request reprint The urokinase plasminogen activator (uPA) system in uterine natural killer cells in the placental bed during early pregnancy
    K Naruse
    Institute of Cellular Medicine, Newcastle University, Newcastle upon Tyne, UK
    Placenta 30:398-404. 2009
    ..PAI-1 and PAI-2 were not detected in uNK cell culture supernatants by Western blot analysis. These results suggest that uNK cells may regulate EVT invasion and spiral artery remodeling via the uPA system...
  23. doi request reprint Localization of angiogenic growth factors and their receptors in the human placental bed throughout normal human pregnancy
    B Schiessl
    School of Surgical and Reproductive Sciences, Newcastle University, Framlington Place, Newcastle upon Tyne NE2 4HH, UK
    Placenta 30:79-87. 2009
    ..These findings suggest that the Ang and VEGF families may play a role in the process of spiral artery remodelling in normal pregnancy...
  24. ncbi request reprint Vascular endothelial growth factor but not placental growth factor promotes trophoblast syncytialization in vitro
    I P Crocker
    MRC Development Group, School of Human Development, Department of Obstetrics and Gynaecology, University of Nottingham, City Hospital, Nottingham, United Kingdom
    J Soc Gynecol Investig 8:341-6. 2001
    ..The object of this study was to determine the effect of epithelial growth factor (EGF), vascular endothelial growth factor (VEGF), and placental growth factor (PlGF) on the differentiation of first-trimester and term cytotrophoblasts...
  25. doi request reprint Effects of local decidua on trophoblast invasion and spiral artery remodeling in focal placenta creta - an immunohistochemical study
    T Hannon
    Reproductive and Vascular Biology Group, Institute of Cellular Medicine, Newcastle University, Newcastle upon Tyne NE2 4HH, United Kingdom
    Placenta 33:998-1004. 2012
    ..The study aims to compare differences in migratory trophoblast and spiral artery remodeling between areas with and without decidua at the placental implantation site...
  26. doi request reprint Role of interleukin 8 in uterine natural killer cell regulation of extravillous trophoblast cell invasion
    L G de Oliveira
    Institute of Cellular Medicine, Newcastle University, Newcastle upon Tyne, UK
    Placenta 31:595-601. 2010
    ..Decidual factors, including uterine natural killer (uNK) and T cell derived cytokines play a role in regulating this process. Interleukin (IL) 8 has been implicated as a regulator of EVT invasion...
  27. doi request reprint IFPA Meeting 2011 workshop report II: Angiogenic signaling and regulation of fetal endothelial function; placental and fetal circulation and growth; spiral artery remodeling
    J N Bulmer
    Reproductive and Vascular Biology Group, Institute of Cellular Medicine, Newcastle University, Newcastle upon Tyne, UK
    Placenta 33:S9-S14. 2012
    ....
  28. doi request reprint Immune cells in the placental bed
    Judith N Bulmer
    Institute of Cellular Medicine, Newcastle University, Newcastle upon Tyne, UK
    Int J Dev Biol 54:281-94. 2010
    ....
  29. ncbi request reprint Human uterine natural killer cells: a reappraisal
    Judith N Bulmer
    School of Clinical and Laboratory Sciences, University of Newcastle upon Tyne, Framlington Place, Newcastle upon Tyne NE2 4HH, UK
    Mol Immunol 42:511-21. 2005
    ....
  30. ncbi request reprint Effect of hypoxia on cellular adhesion to vitronectin and fibronectin
    G E Lash
    Department of Anatomy and Cell Biology, Queen's University, Kingston, Ontario, K7L 3N6, Canada
    Biochem Biophys Res Commun 287:622-9. 2001
    ..These findings suggest an important role for oxygen in the regulation of cellular invasion, possibly in part through its effects on integrin and uPAR-mediated mechanisms of adhesion...
  31. doi request reprint Localization of matrix metalloproteinase (MMP)-2, MMP-9 and tissue inhibitors for MMPs (TIMPs) in uterine natural killer cells in early human pregnancy
    Katsuhiko Naruse
    Institute of Cellular Medicine, Newcastle University, Newcastle upon Tyne, UK
    Hum Reprod 24:553-61. 2009
    ..Uterine natural killer (uNK) cells appear to be a major maternal regulator of placentation through the secretion of growth factors, cytokines and proteinases...
  32. ncbi request reprint Tumour cell dissemination following endoscopic stent insertion
    K Maruthachalam
    Department of Colorectal Surgery, Freeman Hospital, Newcastle upon Tyne, UK
    Br J Surg 94:1151-4. 2007
    ..This study examined whether colonoscopy or endoscopic stent insertion increases levels of carcinoembryonic antigen (CEA) and/or cytokeratin (CK) 20 mRNA expression in the peripheral circulation of patients with colorectal cancer...
  33. ncbi request reprint Inhibition of breast carcinoma and trophoblast cell invasiveness by vascular endothelial growth factor
    Tania E Fitzpatrick
    Department of Anatomy and Cell Biology, Queen s University, Kingston, Ontario, Canada K7L 3N6
    Exp Cell Res 283:247-55. 2003
    ..These results indicate that the biological actions of VEGF on certain cell types may differ from the effects of this molecule on vascular endothelial cells, and therefore are relevant to angiogenesis-based therapies...
  34. ncbi request reprint Oxygen-mediated regulation of tumor cell invasiveness. Involvement of a nitric oxide signaling pathway
    Lynne Marie Postovit
    Department of Anatomy and Cell Biology, Queen s University, Kingston, Ontario K7L 3N6, Canada
    J Biol Chem 277:35730-7. 2002
    ..These findings reveal that an important mechanism by which hypoxia increases tumor cell invasiveness (and possibly metastasis) requires inhibition of the nitric oxide signaling pathway involving sGC and PKG activation...
  35. ncbi request reprint Oxygen as a regulator of cellular phenotypes in pregnancy and cancer
    Gendie E Lash
    Department of Anatomy, Queen s University, Kingston, Canada
    Can J Physiol Pharmacol 80:103-9. 2002
    ....
  36. ncbi request reprint Inhibition of human trophoblast invasiveness by high glucose concentrations
    Louiza Belkacemi
    Department of Anatomy and Cell Biology, Botterell Hall, 9th Floor, Queen s University, Kingston, Ontario, Canada K7L 3N6
    J Clin Endocrinol Metab 90:4846-51. 2005
    ..Trophoblast invasion of the uterus is regulated by local microenvironmental factors...
  37. ncbi request reprint Heme oxygenase expression in selected regions of term human placenta
    Brian E McLaughlin
    Departments of Pharmacology, Faculty of Health Sciences, Queen s University, Kingston, Ontario, Canada K7L 3N6
    Exp Biol Med (Maywood) 228:564-7. 2003
    ..Microsomal HO enzymatic activity correlated with HO-2, but not HO-1, protein content...
  38. ncbi request reprint Nitric oxide-mediated regulation of hypoxia-induced B16F10 melanoma metastasis
    Lynne Marie Postovit
    Department of Anatomy and Cell Biology, Queen s University, Kingston, ON, Canada
    Int J Cancer 108:47-53. 2004
    ..These findings suggest that a novel mechanism whereby hypoxia regulates metastatic potential involves a downstream inhibition of cGMP-dependent NO signaling...