Angus I Lamond

Summary

Affiliation: University of Dundee
Country: UK

Publications

  1. pmc Cajal bodies and coilin--moving towards function
    Stephen C Ogg
    Wellcome Trust Biocentre, University of Dundee, Dundee DD1 5EH Scotland, United Kingdom
    J Cell Biol 159:17-21. 2002
  2. pmc Quantitative kinetic analysis of nucleolar breakdown and reassembly during mitosis in live human cells
    Anthony Kar Lun Leung
    Division of Gene Regulation and Expression, School of Life Sciences, Wellcome Trust Biocentre, University of Dundee, Dundee DD1 5EH, Scotland, UK
    J Cell Biol 166:787-800. 2004
  3. pmc In vivo analysis of NHPX reveals a novel nucleolar localization pathway involving a transient accumulation in splicing speckles
    Anthony K L Leung
    Wellcome Trust Biocentre, MSI WTB complex, University of Dundee, Dundee DD1 5EH, Scotland, UK
    J Cell Biol 157:615-29. 2002
  4. pmc A perspective on proteomics in cell biology
    Yasmeen Ahmad
    Centre for Gene Regulation and Expression, College of Life Sciences, University of Dundee, Dow Street, Dundee DD1 5EH, Scotland, UK
    Trends Cell Biol 24:257-64. 2014
  5. pmc Advancing cell biology through proteomics in space and time (PROSPECTS)
    Angus I Lamond
    Centre for Gene Regulation and Expression, College of Life Sciences, University of Dundee, Dundee DD1 5EH United Kingdom
    Mol Cell Proteomics 11:O112.017731. 2012
  6. ncbi request reprint Structure and function in the nucleus
    A I Lamond
    Department of Biochemistry, University of Dundee, Dundee DD1 4HN, Scotland, UK
    Science 280:547-53. 1998
  7. ncbi request reprint Nuclear speckles: a model for nuclear organelles
    Angus I Lamond
    Wellcome Trust Biocentre, Medical Sciences Institute Wellcome Trust Biocentre Complex, University of Dundee, Dundee DD1 5EH, UK
    Nat Rev Mol Cell Biol 4:605-12. 2003
  8. pmc Nuclear processes controlled by molecular machines
    Archa H Fox
    Wellcome Trust Biocentre, University of Dundee, Dundee DD1 5EH, UK
    Genome Biol 3:REPORTS4016. 2002
  9. ncbi request reprint Cajal body proteins SMN and Coilin show differential dynamic behaviour in vivo
    Judith E Sleeman
    University of Dundee, MSI WTB complex, School of Life Sciences, Dow Street, Dundee DD1 5EH, UK
    J Cell Sci 116:2039-50. 2003
  10. pmc Direct interaction between hnRNP-M and CDC5L/PLRG1 proteins affects alternative splice site choice
    David Lleres
    Wellcome Trust Centre for Gene Regulation and Expression, College of Life Sciences, University of Dundee, MSI WTB JBC Complex, Dow Street, Dundee DD1 5EH, UK
    EMBO Rep 11:445-51. 2010

Detail Information

Publications85

  1. pmc Cajal bodies and coilin--moving towards function
    Stephen C Ogg
    Wellcome Trust Biocentre, University of Dundee, Dundee DD1 5EH Scotland, United Kingdom
    J Cell Biol 159:17-21. 2002
    ..The Cajal body is an example of a conserved nuclear compartment that has been linked to molecular disease. Recent studies have shown Cajal bodies to be surprisingly mobile and offer clues about their function in the cell...
  2. pmc Quantitative kinetic analysis of nucleolar breakdown and reassembly during mitosis in live human cells
    Anthony Kar Lun Leung
    Division of Gene Regulation and Expression, School of Life Sciences, Wellcome Trust Biocentre, University of Dundee, Dundee DD1 5EH, Scotland, UK
    J Cell Biol 166:787-800. 2004
    ....
  3. pmc In vivo analysis of NHPX reveals a novel nucleolar localization pathway involving a transient accumulation in splicing speckles
    Anthony K L Leung
    Wellcome Trust Biocentre, MSI WTB complex, University of Dundee, Dundee DD1 5EH, Scotland, UK
    J Cell Biol 157:615-29. 2002
    ..The data show a specific temporal pathway involving the sequential and directed accumulation of NHPX in distinct subnuclear compartments, and define a novel mechanism for nucleolar localization...
  4. pmc A perspective on proteomics in cell biology
    Yasmeen Ahmad
    Centre for Gene Regulation and Expression, College of Life Sciences, University of Dundee, Dow Street, Dundee DD1 5EH, Scotland, UK
    Trends Cell Biol 24:257-64. 2014
    ....
  5. pmc Advancing cell biology through proteomics in space and time (PROSPECTS)
    Angus I Lamond
    Centre for Gene Regulation and Expression, College of Life Sciences, University of Dundee, Dundee DD1 5EH United Kingdom
    Mol Cell Proteomics 11:O112.017731. 2012
    ....
  6. ncbi request reprint Structure and function in the nucleus
    A I Lamond
    Department of Biochemistry, University of Dundee, Dundee DD1 4HN, Scotland, UK
    Science 280:547-53. 1998
    ..This review summarizes recent progress in understanding nuclear organization, highlighting in particular the dynamic aspects of nuclear structure...
  7. ncbi request reprint Nuclear speckles: a model for nuclear organelles
    Angus I Lamond
    Wellcome Trust Biocentre, Medical Sciences Institute Wellcome Trust Biocentre Complex, University of Dundee, Dundee DD1 5EH, UK
    Nat Rev Mol Cell Biol 4:605-12. 2003
    ..Studies on the composition, structure and behaviour of speckles have provided a model for understanding the functional compartmentalization of the nucleus and the organization of the gene-expression machinery...
  8. pmc Nuclear processes controlled by molecular machines
    Archa H Fox
    Wellcome Trust Biocentre, University of Dundee, Dundee DD1 5EH, UK
    Genome Biol 3:REPORTS4016. 2002
  9. ncbi request reprint Cajal body proteins SMN and Coilin show differential dynamic behaviour in vivo
    Judith E Sleeman
    University of Dundee, MSI WTB complex, School of Life Sciences, Dow Street, Dundee DD1 5EH, UK
    J Cell Sci 116:2039-50. 2003
    ..This in vivo study indicates that SMN and coilin interact differentially with Cajal bodies and reveals parallels in the pathway for reassembly of nucleoli and Cajal bodies following mitosis...
  10. pmc Direct interaction between hnRNP-M and CDC5L/PLRG1 proteins affects alternative splice site choice
    David Lleres
    Wellcome Trust Centre for Gene Regulation and Expression, College of Life Sciences, University of Dundee, MSI WTB JBC Complex, Dow Street, Dundee DD1 5EH, UK
    EMBO Rep 11:445-51. 2010
    ..hnRNP-M affects both 5' and 3' alternative splice site choices, and an hnRNP-M mutant lacking the CDC5L/PLRG1 interaction domain is unable to modulate alternative splicing of an adeno-E1A mini-gene substrate...
  11. pmc FRET analyses of the U2AF complex localize the U2AF35/U2AF65 interaction in vivo and reveal a novel self-interaction of U2AF35
    Janet Chusainow
    Wellcome Trust Biocentre, University of Dundee, Dow Street, Dundee DD1 5EH, UK
    RNA 11:1201-14. 2005
    ..The data show that FRET studies offer a valuable approach for probing interactions between pre-mRNA splicing factors in vivo...
  12. pmc A proteomic screen for nucleolar SUMO targets shows SUMOylation modulates the function of Nop5/Nop58
    Belinda J Westman
    Wellcome Trust Centre for Gene Regulation and Expression, College of Life Sciences, University of Dundee, Dundee DD15EH, UK
    Mol Cell 39:618-31. 2010
    ..5K proteins appear not to be SUMO targets. SUMOylation is essential for high-affinity Nop58 binding to snoRNAs. This study provides direct evidence linking SUMO modification with snoRNP function...
  13. pmc Characterization of native protein complexes and protein isoform variation using size-fractionation-based quantitative proteomics
    Kathryn J Kirkwood
    Centre for Gene Regulation and Expression, College of Life Sciences, University of Dundee, Dow St, Dundee, DD1 5EH, United Kingdom
    Mol Cell Proteomics 12:3851-73. 2013
    ..All of these SEC data on the native separation of protein complexes have been integrated within the Encyclopedia of Proteome Dynamics, an online, multidimensional data-sharing resource available to the community. ..
  14. doi request reprint HSP90 and its R2TP/Prefoldin-like cochaperone are involved in the cytoplasmic assembly of RNA polymerase II
    Séverine Boulon
    Wellcome Trust Centre for Gene Regulation and Expression, University of Dundee, Dundee, UK
    Mol Cell 39:912-24. 2010
    ..hSpagh also bound the free RPA194 subunit of RNA polymerase I, suggesting a general role in assembling RNA polymerases...
  15. pmc Analysis of human small nucleolar RNAs (snoRNA) and the development of snoRNA modulator of gene expression vectors
    Motoharu Ono
    Wellcome Trust Centre for Gene Regulation and Expression, and Division of Biological Chemistry and Drug Discovery, College of Life Sciences, University of Dundee, Dow Street, Dundee DD1 5EH, United Kingdom
    Mol Biol Cell 21:1569-84. 2010
    ..We foresee applications for snoMEN vectors in basic gene expression research, target validation, and gene therapy...
  16. pmc A quantitative spatial proteomics analysis of proteome turnover in human cells
    Francois Michel Boisvert
    Wellcome Trust Centre for Gene Regulation and Expression, College of Life Sciences, University of Dundee, Dundee DD1 5EH, United Kingdom
    Mol Cell Proteomics 11:M111.011429. 2012
    ..This strongly correlated with subunits of large, multiprotein complexes, suggesting a general mechanism whereby their assembly is controlled in a different subcellular location to their main site of function...
  17. ncbi request reprint A proteomic study of SUMO-2 target proteins
    Alfred C O Vertegaal
    Wellcome Trust Biocentre, University of Dundee, Dundee DD1 5EH, United Kingdom
    J Biol Chem 279:33791-8. 2004
    ..SART1 and heterogeneous nuclear RNP M were both shown to be genuine SUMO targets, confirming the validity of the approach...
  18. pmc Establishment of a protein frequency library and its application in the reliable identification of specific protein interaction partners
    Séverine Boulon
    The Wellcome Trust Centre for Gene Regulation and Expression, University of Dundee, Dundee DD1 5EH, Scotland, United Kingdom
    Mol Cell Proteomics 9:861-79. 2010
    ....
  19. pmc P54nrb forms a heterodimer with PSP1 that localizes to paraspeckles in an RNA-dependent manner
    Archa H Fox
    Division of Gene Regulation and Expression, Wellcome Trust Biocentre, University of Dundee, Dundee DD1 5EH, United Kingdom
    Mol Biol Cell 16:5304-15. 2005
    ..Thus paraspeckles are the sites where a subset of the total cellular pool of p54nrb is targeted in a RNA Polymerase II-dependent manner...
  20. pmc Analysis of nucleolar protein dynamics reveals the nuclear degradation of ribosomal proteins
    Yun Wah Lam
    Division of Gene Regulation and Expression, Wellcome Trust Biocentre, College of Life Sciences, University of Dundee, Dow Street, Dundee DD1 5EH, UK
    Curr Biol 17:749-60. 2007
    ....
  21. ncbi request reprint Protein phosphatase 4 interacts with the Survival of Motor Neurons complex and enhances the temporal localisation of snRNPs
    Graeme K Carnegie
    Medical Research Council Protein Phosphorylation Unit, Division of Cell Signalling, University of Dundee, Dundee DD1 5EH, Scotland, UK
    J Cell Sci 116:1905-13. 2003
    ..Expression of GFP-R2-PPP4c in HeLa cells enhances the temporal localisation of newly formed snRNPs, which is consistent with an association of R2-PPP4c with the SMN protein complex...
  22. pmc Systematic analysis of protein pools, isoforms, and modifications affecting turnover and subcellular localization
    Yasmeen Ahmad
    Wellcome Trust Centre for Gene Regulation and Expression, College of Life Sciences, University of Dundee, Dundee DD1 5EH United Kingdom
    Mol Cell Proteomics 11:M111.013680. 2012
    ..Collectively, these experimental and analytical approaches provide a framework for expanding the functional annotation of the genome...
  23. pmc Characterization of MRFAP1 turnover and interactions downstream of the NEDD8 pathway
    Mark Larance
    Wellcome Trust Centre for Gene Regulation and Expression, College of Life Sciences, University of Dundee, Dundee, DD1 5EH, United Kingdom
    Mol Cell Proteomics 11:M111.014407. 2012
    ..These data highlight an important new arm of the NEDD8-cullin pathway...
  24. pmc Spatial mapping of splicing factor complexes involved in exon and intron definition
    Jonathan D Ellis
    Medical Research Council Human Genetics Unit, Western General Hospital, Edinburgh EH4 2XU, Scotland, UK
    J Cell Biol 181:921-34. 2008
    ..Spatial mapping using FLIM-FRET reveals differences in splicing factors interactions within complexes located in separate subnuclear domains...
  25. pmc Repo-Man recruits PP1 gamma to chromatin and is essential for cell viability
    Laura Trinkle-Mulcahy
    University of Dundee, Dundee DD1 5EH, Scotland, UK
    J Cell Biol 172:679-92. 2006
    ..The data indicate that Repo-Man forms an essential complex with PP1gamma and is required for the recruitment of PP1 to chromatin...
  26. ncbi request reprint Toward a high-resolution view of nuclear dynamics
    Laura Trinkle-Mulcahy
    Wellcome Trust Centre for Gene Regulation and Expression, College of Life Sciences, University of Dundee, Dundee DD1 5EH, UK
    Science 318:1402-7. 2007
    ..Here, we review the impact of new technologies, especially in areas of fluorescence microscopy and proteomics, which are providing major insights into dynamic processes affecting both structure and function within the nucleus...
  27. ncbi request reprint Paraspeckles: a novel nuclear domain
    Archa H Fox
    Wellcome Trust Biocentre, MSI WTB complex, University of Dundee, DD1 4HN, Dundee, United Kingdom
    Curr Biol 12:13-25. 2002
    ..Many nuclear proteins are known to interact dynamically with one or other of these bodies, and disruption of the specific organization of nuclear proteins can result in defects in cell functions and may cause molecular disease...
  28. pmc Global subcellular characterization of protein degradation using quantitative proteomics
    Mark Larance
    Centre for Gene Regulation and Expression, College of Life Sciences, University of Dundee, Dow St, Dundee, United Kingdom
    Mol Cell Proteomics 12:638-50. 2013
    ..Bioinformatic analysis of the entire data set is presented in the Encyclopedia of Proteome Dynamics, a web-based resource, with proteins annotated for stability and subcellular distribution...
  29. ncbi request reprint The multifunctional nucleolus
    Francois Michel Boisvert
    Wellcome Trust Biocentre, University of Dundee, Dundee DD1 5EH, UK
    Nat Rev Mol Cell Biol 8:574-85. 2007
    ....
  30. pmc FMN2 is a novel regulator of the cyclin-dependent kinase inhibitor p21
    Kayo Yamada
    Centre for Gene Regulation and Expression College of Life Sciences University of Dundee Dundee, Scotland, UK
    Cell Cycle 12:2348-54. 2013
    ..In addition, we also investigate the role of RelA's threonine 505 residue in the control of FMN2. Our results identify FMN2 as a crucial protein involved in the control of p21. ..
  31. pmc Analysis of human protein replacement stable cell lines established using snoMEN-PR vector
    Motoharu Ono
    Centre for Gene Regulation and Expression, College of Life Sciences, University of Dundee, Dundee, United Kingdom
    PLoS ONE 8:e62305. 2013
    ..The snoMEN approach facilitates the study of mammalian proteins, particularly those that have so far been difficult to investigate by exogenous expression and has wide applications in basic and applied gene-expression research...
  32. pmc Identification of human miRNA precursors that resemble box C/D snoRNAs
    Motoharu Ono
    Wellcome Trust Centre for Gene Regulation and Expression, College of Life Sciences, University of Dundee, Dow Street, Dundee DD1 5EH, UK
    Nucleic Acids Res 39:3879-91. 2011
    ..The data suggest that a subset of small regulatory RNAs may have evolved from box C/D snoRNAs...
  33. pmc UV-induced fragmentation of Cajal bodies
    Mario Cioce
    Gene Regulation and Expression Division, University of Dundee, Dundee DD1 5EH, Scotland, UK
    J Cell Biol 175:401-13. 2006
    ..These data demonstrate that CBs are specific nuclear targets of cellular stress-response pathways and identify PA28gamma as a novel regulator of CB integrity...
  34. pmc Identification of peptide inhibitors of pre-mRNA splicing derived from the essential interaction domains of CDC5L and PLRG1
    Paul Ajuh
    The University of Dundee, School of Life Sciences, Wellcome Trust Biocentre, Dow Street, Dundee DD1 5EH, UK
    Nucleic Acids Res 31:6104-16. 2003
    ..The results from this study indicate that the interaction between CDC5L and PLRG1 is essential for pre-mRNA splicing and further demonstrate that small peptides can be used as effective splicing inhibitors...
  35. pmc NOPdb: Nucleolar Proteome Database
    Anthony Kar Lun Leung
    Division of Gene Regulation and Expression, Wellcome Trust Biocentre, School of Life Sciences, University of Dundee, Dundee DD1 5EH, UK
    Nucleic Acids Res 34:D218-20. 2006
    ..g. LocusLink, OMIM and PubMed)...
  36. pmc An intranucleolar body associated with rDNA
    Saskia Hutten
    Wellcome Trust Centre for Gene Regulation and Expression, College of Life Sciences, University of Dundee, UK
    Chromosoma 120:481-99. 2011
    ..INBs are abundant in nucleoli of unstressed cells during S phase and localise in close proximity to rDNA with heterochromatic features. The data suggest the INB is linked with regulation of rDNA transcription and/or maintenance of rDNA...
  37. ncbi request reprint The dynamics of the nucleolus
    Anthony K L Leung
    Division of Gene Regulation and Expression, School of Life Sciences, Wellcome Trust Biocentre, University of Dundee, Dundee DD1 5EH, Scotland, UK
    Crit Rev Eukaryot Gene Expr 13:39-54. 2003
    ..This review focuses on nucleolar dynamics, studied at each of the single protein, collective proteome, and subnucleolar organization levels...
  38. pmc Time-lapse imaging reveals dynamic relocalization of PP1gamma throughout the mammalian cell cycle
    Laura Trinkle-Mulcahy
    Wellcome Trust Biocentre, University of Dundee, Dundee DD1 5EH, United Kingdom
    Mol Biol Cell 14:107-17. 2003
    ..The changing spatio-temporal distribution of PP1gamma revealed using the stable PP1 cell lines implicates it in multiple processes, including nucleolar function, the regulation of chromosome segregation and cytokinesis...
  39. pmc PHD1 links cell-cycle progression to oxygen sensing through hydroxylation of the centrosomal protein Cep192
    Sandra C Moser
    Centre for Gene Regulation and Expression, College of Life Sciences, University of Dundee, Dundee DD1 5EH, UK
    Dev Cell 26:381-92. 2013
    ..By modulating Cep192 levels, PHD1 thereby affects the processes of centriole duplication and centrosome maturation and contributes to the regulation of cell-cycle progression. ..
  40. pmc Human miRNA precursors with box H/ACA snoRNA features
    Michelle S Scott
    Division of Biological Chemistry and Drug Discovery, College of Life Sciences, University of Dundee, Dundee, United Kingdom
    PLoS Comput Biol 5:e1000507. 2009
    ..The detection of small RNA molecules that share features of miRNAs and snoRNAs suggest that these classes of RNA may have an evolutionary relationship...
  41. ncbi request reprint Nuclear organisation and subnuclear bodies
    Melpomeni Platani
    Wellcome Trust Biocentre, MSI WTB complex, DD1 5EH, Dundee, Scotland, United Kingdom
    Prog Mol Subcell Biol 35:1-22. 2004
  42. pmc A quantitative proteomics analysis of subcellular proteome localization and changes induced by DNA damage
    Francois Michel Boisvert
    The Wellcome Trust Centre for Gene Regulation and Expression, University of Dundee, MSI WTB JBC Complex, Dow Street, Dundee DD1 5EH, Scotland, United Kingdom
    Mol Cell Proteomics 9:457-70. 2010
    ..DNA damage was found to cause dissociation of the proteasome from inhibitory proteins and assembly chaperones in the cytoplasm and relocation to associate with proteasome activators in the nucleus...
  43. pmc Identification and functional characterization of FMN2, a regulator of the cyclin-dependent kinase inhibitor p21
    Kayo Yamada
    Centre for Gene Regulation and Expression, College of Life Sciences, University of Dundee, Dundee, UK
    Mol Cell 49:922-33. 2013
    ..FMN2 is also induced by activation of other oncogenes, hypoxia, and DNA damage. These results identify FMN2 as a crucial component in the regulation of p21 and consequent oncogene/stress-induced cell-cycle arrest in human cells...
  44. pmc Bioinformatic analysis of the nucleolus
    Anthony K L Leung
    Division of Gene Regulation and Expression, School of Life Sciences, Wellcome Trust Biocentre, University of Dundee, Scotland, UK
    Biochem J 376:553-69. 2003
    ....
  45. pmc NOPdb: Nucleolar Proteome Database--2008 update
    Yasmeen Ahmad
    Wellcome Trust Centre for Gene Regulation and Expression and School of Computing, University of Dundee, Dundee DD1 5EH, UK
    Nucleic Acids Res 37:D181-4. 2009
    ....
  46. pmc Characterization and prediction of protein nucleolar localization sequences
    Michelle S Scott
    Division of Biological Chemistry and Drug Discovery and Wellcome Trust Centre for Gene Regulation and Expression, College of Life Sciences, University of Dundee, Dow Street, Dundee DD1 5EH, UK
    Nucleic Acids Res 38:7388-99. 2010
    ..NoLSs are a prevalent type of targeting motif that is distinct from nuclear localization signals and that can be computationally predicted...
  47. ncbi request reprint Detecting protein-protein interactions in vivo with FRET using multiphoton fluorescence lifetime imaging microscopy (FLIM)
    David Lleres
    Wellcome Trust Biocentre, College of Life Sciences, University of Dundee, United Kingdom
    Curr Protoc Cytom . 2007
    ..A detailed protocol describing the complete microscope procedure and the choice of the appropriate experimental controls as well as the FRET calculations is also included...
  48. ncbi request reprint Nuclear functions in space and time: gene expression in a dynamic, constrained environment
    Laura Trinkle-Mulcahy
    Centre for Gene Regulation and Expression, MSI WTB complex, University of Dundee, Dundee DD1 5EH, Scotland, United Kingdom
    FEBS Lett 582:1960-70. 2008
    ..In several cases, as highlighted here, this has turned up surprising results and has forced a re-evaluation of models for nuclear structure and gene regulation...
  49. pmc Large-scale isolation of Cajal bodies from HeLa cells
    Yun Wah Lam
    Wellcome Trust Biocentre, MSI WTB complex, University of Dundee, Dundee DD1 4HN, Scotland, United Kingdom
    Mol Biol Cell 13:2461-73. 2002
    ....
  50. ncbi request reprint Nuclear substructure and dynamics
    Angus I Lamond
    Wellcome Trust Biocentre, MSI WTB complex, University of Dundee, UK
    Curr Biol 13:R825-8. 2003
  51. pmc Human box C/D snoRNA processing conservation across multiple cell types
    Michelle S Scott
    Division of Biological Chemistry and Drug Discovery, College of Life Sciences, University of Dundee, Dow Street, Dundee DD1 5EH, UK
    Nucleic Acids Res 40:3676-88. 2012
    ..Functional analyses demonstrated that this region of HBII-180C can influence the alternative splicing of FGFR3 pre-mRNA, supporting a role for some snoRNAs in the regulation of splicing...
  52. pmc PNAC: a protein nucleolar association classifier
    Michelle S Scott
    Division of Biological Chemistry and Drug Discovery, College of Life Sciences, University of Dundee, Dow Street, Dundee DD1 5EH, UK
    BMC Genomics 12:74. 2011
    ..However, in most cases, we know neither the fraction of each protein pool that is nucleolus-associated nor whether their association is permanent or conditional...
  53. pmc Mass spectrometry-based immuno-precipitation proteomics - the user's guide
    Sara Ten Have
    Wellcome Trust Centre for Gene Regulation and Expression, College of Life Sciences, University of Dundee, Dundee, Scotland, UK
    Proteomics 11:1153-9. 2011
    ..Detailed experimental methodologies have been referenced but not described in the form of protocols...
  54. pmc High-resolution whole-genome sequencing reveals that specific chromatin domains from most human chromosomes associate with nucleoli
    Silvana van Koningsbruggen
    Wellcome Trust Centre for Gene Regulation and Expression, College of Life Sciences, University of Dundee, Dundee DD1 5EH, United Kingdom
    Mol Biol Cell 21:3735-48. 2010
    ..Unexpectedly, both the direct DNA sequencing and fluorescence photoactivation data show that certain chromatin loci can specifically associate with either the nucleolus, or the nuclear envelope...
  55. pmc The nucleolus under stress
    Séverine Boulon
    Wellcome Trust Centre for Gene Regulation and Expression, University of Dundee, Dundee DD1 5EH, UK
    Mol Cell 40:216-27. 2010
    ..Crosstalk between nucleoli and CBs is also discussed in the context of stress responses...
  56. pmc Quantitative analysis of chromatin compaction in living cells using FLIM-FRET
    David Lleres
    Wellcome Trust Centre for Gene Regulation and Expression, College of Life Sciences, University of Dundee, Dundee DD1 5EH, Scotland, UK
    J Cell Biol 187:481-96. 2009
    ..These data are consistent with extensive higher order folding of chromatin fibers taking place during anaphase...
  57. pmc Stable-isotope labeling with amino acids in nematodes
    Mark Larance
    Wellcome Trust Centre for Gene Regulation and Expression, University of Dundee, Dundee, UK
    Nat Methods 8:849-51. 2011
    ..Combining our approach with quantitative proteomics methods, we characterized the heat-shock response in worms...
  58. pmc A role for SUMOylation in snoRNP biogenesis revealed by quantitative proteomics
    Belinda J Westman
    Wellcome Trust Centre for Gene Regulation and Expression, College of Life Sciences, University of Dundee, UK
    Nucleus 2:30-7. 2011
    ....
  59. pmc A novel function for human factor C1 (HCF-1), a host protein required for herpes simplex virus infection, in pre-mRNA splicing
    Paul Ajuh
    School of Life Sciences, The University of Dundee, Dow Street, Dundee DD1 5EH, UK
    EMBO J 21:6590-602. 2002
    ..The inhibition of splicing in tsBN67 cells correlates with the temperature-sensitive cell cycle arrest phenotype, suggesting that HCF-1-dependent splicing events may be required for cell cycle progression...
  60. ncbi request reprint The nucleolus
    Yun Wah Lam
    Wellcome Trust Biocentre, MSI WTB complex, University of Dundee, Dundee, DD1 5EH, UK
    J Cell Sci 118:1335-7. 2005
  61. pmc p53-Dependent subcellular proteome localization following DNA damage
    Francois Michel Boisvert
    Wellcome Trust Centre for Gene Regulation and Expression, College of Life Sciences, University of Dundee, MSI WTB JBC Complex, Dundee, UK
    Proteomics 10:4087-97. 2010
    ....
  62. ncbi request reprint Cajal body dynamics and association with chromatin are ATP-dependent
    Melpomeni Platani
    School of Life Sciences, Division of Gene Regulation and Expression, University of Dundee, Medical Sciences Institute Wellcome Trust Biocentre, Dow Street, Dundee, DD1 5EH, Scotland
    Nat Cell Biol 4:502-8. 2002
    ..This behaviour is fundamentally different from the ATP-dependent mobility observed for chromatin and suggests that a novel mechanism governs CB, and possibly other, nuclear body dynamics...
  63. ncbi request reprint Mitotic phosphatases: no longer silent partners
    Laura Trinkle-Mulcahy
    Division of Gene Regulation and Expression, School of Life Sciences, University of Dundee, Dow St, Dundee DD1 5EH, UK
    Curr Opin Cell Biol 18:623-31. 2006
    ..These exciting advances show that protein phosphatases are not merely silent partners to kinases in regulating the control of cell division...
  64. pmc Cleaning up the masses: exclusion lists to reduce contamination with HPLC-MS/MS
    Kelly Hodge
    Centre for Gene Regulation and Expression, College of Life Sciences, University of Dundee, Dow Street, Dundee DD1 5EH, United Kingdom
    J Proteomics 88:92-103. 2013
    ..Here we show that by employing these empirically generated lists, it was possible to reduce the time spent analysing contaminating peptides in a given sample thereby facilitating more efficient data acquisition and analysis...
  65. ncbi request reprint Splicing regulation at the second catalytic step by Sex-lethal involves 3' splice site recognition by SPF45
    María José Lallena
    Gene Expression Programme, European Molecular Biology Laboratory, Meyerhofstrasse 1, D 69117, Heidelberg, Germany
    Cell 109:285-96. 2002
    ..A similar role for SPF45 is demonstrated in the activation of a cryptic 3' ss generated by a mutation that causes human beta-thalassemia...
  66. ncbi request reprint Directed proteomic analysis of the human nucleolus
    Jens S Andersen
    Department of Biochemistry and Molecular Biology, University of Southern Denmark, Campusvej 55, DK 5230, Odense M, Denmark
    Curr Biol 12:1-11. 2002
    ..Recent studies suggest it may also have roles in RNA transport, RNA modification, and cell cycle regulation. Despite over 150 years of research into nucleoli, many aspects of their structure and function remain uncharacterized...
  67. ncbi request reprint hLodestar/HuF2 interacts with CDC5L and is involved in pre-mRNA splicing
    Deana Leonard
    Department of Molecular Genetics, M D Anderson Cancer Center, The University of Texas, Houston, TX 77030, USA
    Biochem Biophys Res Commun 308:793-801. 2003
    ..Although many members of the DExH/D helicase superfamily have been linked to pre-mRNA splicing, this is the first SNF2 family member to be implicated in this pathway...
  68. ncbi request reprint The nuclear PP1 interacting protein ZAP3 (ZAP) is a putative nucleoside kinase that complexes with SAM68, CIA, NF110/45, and HNRNP-G
    Annegret Ulke-Lemee
    Department of Biological Sciences, University of Calgary, 2500 University Drive NW, Calgary, Alberta, Canada
    Biochim Biophys Acta 1774:1339-50. 2007
    ..In ZAP3, although this domain is present, it now appears degenerate and functions to bind PP1 through an RVRW docking site located within the domain...
  69. pmc Proteomic analysis of the Arabidopsis nucleolus suggests novel nucleolar functions
    Alison F Pendle
    John Innes Centre, Norwich NR4 7UH, United Kingdom
    Mol Biol Cell 16:260-9. 2005
    ..This association was confirmed by GFP-fusion protein localization. These results raise the possibility that in plants, nucleoli may have additional functions in mRNA export or surveillance...
  70. pmc Two distinct arginine methyltransferases are required for biogenesis of Sm-class ribonucleoproteins
    Graydon B Gonsalvez
    Department of Genetics, School of Medicine, Case Western Reserve University, Cleveland, OH 44106, USA
    J Cell Biol 178:733-40. 2007
    ..Furthermore, we show that the two enzymes function nonredundantly in Sm protein methylation. Lastly, we provide in vivo evidence demonstrating that Sm protein sDMA modification is required for snRNP biogenesis in human cells...
  71. ncbi request reprint Nucleolar proteome dynamics
    Jens S Andersen
    Department of Biochemistry and Molecular Biology, Campusvej 55, DK 5230 Odense M, Denmark
    Nature 433:77-83. 2005
    ....
  72. pmc Condensin and Repo-Man-PP1 co-operate in the regulation of chromosome architecture during mitosis
    Paola Vagnarelli
    Wellcome Trust Centre for Cell Biology, Institute of Cell Biology, University of Edinburgh, Swann Building, King s Buildings, Mayfield Road, Edinburgh EH9 3JR, UK
    Nat Cell Biol 8:1133-42. 2006
    ..This activity, provisionally termed 'regulator of chromosome architecture' (RCA), cooperates with condensin to preserve the characteristic chromosome architecture during mitosis...
  73. pmc Large-scale proteomic analysis of the human spliceosome
    Juri Rappsilber
    Protein Interaction Laboratory in the Center of Experimental Bioinformatics, Department of Biochemistry and Molecular Biology, University of Southern Denmark, DK 5230 Odense M, Denmark
    Genome Res 12:1231-45. 2002
    ..This investigation provides the most detailed inventory of human spliceosome-associated factors to date, and the data indicate a number of interesting links coordinating splicing with other steps in the gene expression pathway...
  74. ncbi request reprint Characterization of hCINAP, a novel coilin-interacting protein encoded by a transcript from the transcription factor TAFIID32 locus
    Niovi Santama
    Department of Biological Sciences, University of Cyprus and Cyprus Institute of Neurology and Genetics, P O Box 20537, 1678 Nicosia, Cyprus
    J Biol Chem 280:36429-41. 2005
    ..However, hCINAP and TAFIID32 mRNAs are translated from different ATG codons and use distinct reading frames, resulting in them having no identity in their respective protein sequences...
  75. pmc SNEV is an evolutionarily conserved splicing factor whose oligomerization is necessary for spliceosome assembly
    Johannes Grillari
    Institute of Applied Microbiology, University of Natural Resources and Applied Life Sciences Vienna, Austria Muthgasse 18, A 1190 Vienna
    Nucleic Acids Res 33:6868-83. 2005
    ....
  76. ncbi request reprint RNA polymerase II transcription in living color
    Angus I Lamond
    Nat Struct Mol Biol 14:788-90. 2007
  77. ncbi request reprint Cajal bodies: a long history of discovery
    Mario Cioce
    IRBM Merck Research Laboratories Rome, Rome, Italy
    Annu Rev Cell Dev Biol 21:105-31. 2005
    ..We speculate on the relationship between CB function and molecular disease...
  78. pmc In vivo identification of human small ubiquitin-like modifier polymerization sites by high accuracy mass spectrometry and an in vitro to in vivo strategy
    Ivan Matic
    Department of Proteomics and Signal Transduction, Max Planck Institute of Biochemistry, Am Klopferspitz 18, D 82152 Martinsried, Germany
    Mol Cell Proteomics 7:132-44. 2008
    ..The developed methodology is generic and can be adapted for the identification of other sumoylation sites in complex samples...
  79. ncbi request reprint Distinct and overlapping sets of SUMO-1 and SUMO-2 target proteins revealed by quantitative proteomics
    Alfred C O Vertegaal
    Department of Molecular Cell Biology, Leiden University Medical Center, 2300 RC Leiden, The Netherlands
    Mol Cell Proteomics 5:2298-310. 2006
    ....
  80. ncbi request reprint 3D3/lyric: a novel transmembrane protein of the endoplasmic reticulum and nuclear envelope, which is also present in the nucleolus
    Heidi G E Sutherland
    MRC Human Genetics Unit, Western General Hospital, Edinburgh EH4 2XU, UK
    Exp Cell Res 294:94-105. 2004
    ..Furthermore, 3D3/lyric is also found in the nucleolus and is therefore a rare example of a protein that suggests a possible connection between this compartment and the endoplasmic reticulum...
  81. pmc Interaction of U-box E3 ligase SNEV with PSMB4, the beta7 subunit of the 20 S proteasome
    Marlies Löscher
    Institute of Applied Microbiology, University of Natural Resources and Applied Life Sciences, Muthgasse 18, A 1190 Vienna, Austria
    Biochem J 388:593-603. 2005
    ..Additionally, our results provide the first evidence for a physical link between components of the ubiquitin-proteasome system and the spliceosome...
  82. doi request reprint Guidelines for reporting the use of gel electrophoresis in proteomics
    Frank Gibson
    Nat Biotechnol 26:863-4. 2008