Edmund R S Kunji

Summary

Affiliation: University of Cambridge
Country: UK

Publications

  1. doi request reprint Determination of the molecular mass and dimensions of membrane proteins by size exclusion chromatography
    Edmund R S Kunji
    The Medical Research Council, Dunn Human Nutrition Unit, Hills Road, Cambridge CB2 0XY, UK
    Methods 46:62-72. 2008
  2. ncbi request reprint The conserved substrate binding site of mitochondrial carriers
    Edmund R S Kunji
    The Medical Research Council, Dunn Human Nutrition Unit, CB2 2XY, Cambridge, UK
    Biochim Biophys Acta 1757:1237-48. 2006
  3. ncbi request reprint Eukaryotic membrane protein overproduction in Lactococcus lactis
    Edmund R S Kunji
    The Medical Research Council, Dunn Human Nutrition Unit, Hills Road, Wellcome Trust MRC Building, Cambridge CB2 2XY, United Kingdom
    Curr Opin Biotechnol 16:546-51. 2005
  4. ncbi request reprint The role and structure of mitochondrial carriers
    Edmund R S Kunji
    MRC Dunn Human Nutrition Unit, Hills Road, Cambridge CB2 2XY, UK
    FEBS Lett 564:239-44. 2004
  5. ncbi request reprint Projection structure of the atractyloside-inhibited mitochondrial ADP/ATP carrier of Saccharomyces cerevisiae
    Edmund R S Kunji
    Medical Research Council, Dunn Human Nutrition Unit, Hills Road, Cambridge CB2 2XY, United Kingdom
    J Biol Chem 278:36985-8. 2003
  6. ncbi request reprint Lactococcus lactis as host for overproduction of functional membrane proteins
    Edmund R S Kunji
    MRC Dunn Human Nutrition Unit, Hills Road, CB2 2XY Cambridge, UK
    Biochim Biophys Acta 1610:97-108. 2003
  7. pmc Functional expression of eukaryotic membrane proteins in Lactococcus lactis
    Magnus Monné
    The Medical Research Council, Dunn Human Nutrition Unit, Hills Road, Wellcome Trust MRC Building, Cambridge CB2 2XY, UK
    Protein Sci 14:3048-56. 2005
  8. ncbi request reprint Yeast mitochondrial ADP/ATP carriers are monomeric in detergents as demonstrated by differential affinity purification
    Lisa Bamber
    The Medical Research Council, Dunn Human Nutrition Unit, Hills Road, CB2 2XY, Cambridge, UK
    J Mol Biol 371:388-95. 2007
  9. pmc Yeast mitochondrial ADP/ATP carriers are monomeric in detergents
    Lisa Bamber
    Dunn Human Nutrition Unit and Laboratory of Molecular Biology, Medical Research Council, Hills Road, Cambridge CB2 2XY, United Kingdom
    Proc Natl Acad Sci U S A 103:16224-9. 2006
  10. doi request reprint The substrate specificity of the human ADP/ATP carrier AAC1
    John Mifsud
    The Medical Research Council, Mitochondrial Biology Unit, Cambridge, UK
    Mol Membr Biol 30:160-8. 2013

Collaborators

  • Bert Poolman
  • Dirk Jan Slotboom
  • Alan J Robinson
  • Paul G Crichton
  • Airlie J McCoy
  • Eva Pebay-Peyroula
  • Mark van der Giezen
  • T Martin Embley
  • Jorge Tovar
  • David S Horner
  • F Palmieri
  • Gang Ping Xue
  • Shane M Palmer
  • Lisa Bamber
  • Magnus Monné
  • Marilyn Harding
  • Jonathan J Ruprecht
  • John Mifsud
  • Ka Wai Chan
  • Anastasios D Tsaousis
  • Suzanne Floyd
  • Anna R Cappello
  • Italo Stipani
  • Rosita Curcio
  • Anna Rita Cappello
  • Sebastian M J Klaus
  • Alex M Hellawell
  • Stephanie Ravaud
  • Francois Dehez
  • Eva Maria Krammer
  • Chris Chipot
  • Robert P Hirt
  • John M Lucocq
  • Alina V Goldberg
  • Ian M Fearnley
  • Rosemary O'Connor
  • Lucia Daddabbo
  • Daniela V Miniero
  • Christoph Boes
  • Michael E Harbour
  • Alexander Marx
  • Madeline Leahy
  • Anna Ludovico
  • Carlo M T Marobbio
  • Katie O'Callaghan
  • Francesco M Lasorsa
  • Gary Loughran
  • Philipp Stroebel
  • Cedric Favre
  • Giuseppe Trigiante
  • P Jonathan G Butler
  • Daniela Valeria Miniero
  • Stéphane Ravanel
  • Olivier Fabre
  • Fabrice Rebeille
  • Sian Cox
  • Jesse F Gregory
  • Rocío Díaz de la Garza
  • Gilles J C Basset
  • Andrew D Hanson
  • Gloria León-Avila
  • Alexandre Noiriel
  • Gale G Bozzo

Detail Information

Publications29

  1. doi request reprint Determination of the molecular mass and dimensions of membrane proteins by size exclusion chromatography
    Edmund R S Kunji
    The Medical Research Council, Dunn Human Nutrition Unit, Hills Road, Cambridge CB2 0XY, UK
    Methods 46:62-72. 2008
    ..Determining the dimensions of protein-detergent micelles may facilitate membrane protein purification and crystallization by defining the accessibility of the protein surface...
  2. ncbi request reprint The conserved substrate binding site of mitochondrial carriers
    Edmund R S Kunji
    The Medical Research Council, Dunn Human Nutrition Unit, CB2 2XY, Cambridge, UK
    Biochim Biophys Acta 1757:1237-48. 2006
    ..The trigger for the translocation event is likely to be the substrate-induced perturbation of the salt bridge network at the bottom of the cavity...
  3. ncbi request reprint Eukaryotic membrane protein overproduction in Lactococcus lactis
    Edmund R S Kunji
    The Medical Research Council, Dunn Human Nutrition Unit, Hills Road, Wellcome Trust MRC Building, Cambridge CB2 2XY, United Kingdom
    Curr Opin Biotechnol 16:546-51. 2005
    ..Recent advances in the development of the Lactococcus lactis expression system have opened the way for the high-throughput functional expression of eukaryotic membrane proteins...
  4. ncbi request reprint The role and structure of mitochondrial carriers
    Edmund R S Kunji
    MRC Dunn Human Nutrition Unit, Hills Road, Cambridge CB2 2XY, UK
    FEBS Lett 564:239-44. 2004
    ..The projection structure of the yeast ADP/ATP carrier by electron crystallography shows that carriers could form homo-dimers in the membrane...
  5. ncbi request reprint Projection structure of the atractyloside-inhibited mitochondrial ADP/ATP carrier of Saccharomyces cerevisiae
    Edmund R S Kunji
    Medical Research Council, Dunn Human Nutrition Unit, Hills Road, Cambridge CB2 2XY, United Kingdom
    J Biol Chem 278:36985-8. 2003
    ..The AAC3 molecules in the crystal are arranged in symmetrical homo-dimers, but the translocation pore for adenine nucleotides lies in the center of the molecule and not along the dyad axis of the dimer...
  6. ncbi request reprint Lactococcus lactis as host for overproduction of functional membrane proteins
    Edmund R S Kunji
    MRC Dunn Human Nutrition Unit, Hills Road, CB2 2XY Cambridge, UK
    Biochim Biophys Acta 1610:97-108. 2003
    ..These eukaryotic membrane proteins were expressed in a functional form and at levels amenable to structural work...
  7. pmc Functional expression of eukaryotic membrane proteins in Lactococcus lactis
    Magnus Monné
    The Medical Research Council, Dunn Human Nutrition Unit, Hills Road, Wellcome Trust MRC Building, Cambridge CB2 2XY, UK
    Protein Sci 14:3048-56. 2005
    ..The lactococcal expression system may be used for the high-throughput functional characterization of eukaryotic membrane proteins and structural genomics...
  8. ncbi request reprint Yeast mitochondrial ADP/ATP carriers are monomeric in detergents as demonstrated by differential affinity purification
    Lisa Bamber
    The Medical Research Council, Dunn Human Nutrition Unit, Hills Road, CB2 2XY, Cambridge, UK
    J Mol Biol 371:388-95. 2007
    ..Therefore, transport mechanisms need to be considered in which the carrier is operational as a monomer...
  9. pmc Yeast mitochondrial ADP/ATP carriers are monomeric in detergents
    Lisa Bamber
    Dunn Human Nutrition Unit and Laboratory of Molecular Biology, Medical Research Council, Hills Road, Cambridge CB2 2XY, United Kingdom
    Proc Natl Acad Sci U S A 103:16224-9. 2006
    ..Therefore, models of substrate transport have to be considered in which the carrier operates as a monomer rather than as a dimer...
  10. doi request reprint The substrate specificity of the human ADP/ATP carrier AAC1
    John Mifsud
    The Medical Research Council, Mitochondrial Biology Unit, Cambridge, UK
    Mol Membr Biol 30:160-8. 2013
    ..AMP is unlikely to engage fully with all contact points of the substrate binding site, suggesting that it cannot trigger translocation...
  11. pmc Mitochondrial carriers in the cytoplasmic state have a common substrate binding site
    Alan J Robinson
    Dunn Human Nutrition Unit, Medical Research Council, Hills Road, Cambridge CB2 2XY, United Kingdom
    Proc Natl Acad Sci U S A 103:2617-22. 2006
    ..It enables the substrate specificity of uncharacterized mitochondrial carriers to be predicted...
  12. pmc The yeast mitochondrial ADP/ATP carrier functions as a monomer in mitochondrial membranes
    Lisa Bamber
    Medical Research Council, Dunn Human Nutrition Unit, Hills Road, Cambridge CB2 2XY, United Kingdom
    Proc Natl Acad Sci U S A 104:10830-4. 2007
    ..Again, the residual transport activity in the presence of MTSES depended linearly on the amount of cysteine-less carrier. Thus, the entire transport cycle for ADP/ATP exchange is carried out by the monomer...
  13. pmc The mechanism of transport by mitochondrial carriers based on analysis of symmetry
    Alan J Robinson
    Medical Research Council, Dunn Human Nutrition Unit, Hills Road, Cambridge CB2 0XY, United Kingdom
    Proc Natl Acad Sci U S A 105:17766-71. 2008
    ..The interaction energies of the networks allow members of the transporter family to be classified as strict exchangers or uniporters...
  14. doi request reprint Mitochondrial carriers function as monomers
    Edmund R S Kunji
    The Medical Research Council, Mitochondrial Biology Unit, Hills Road, Cambridge, CB2 0XY, UK
    Biochim Biophys Acta 1797:817-31. 2010
    ..We propose a transport model based on the monomer, in which access to a single substrate binding site is controlled by two flanking salt bridge networks, explaining uniport and strict exchange of substrates...
  15. pmc Lipid, detergent, and Coomassie Blue G-250 affect the migration of small membrane proteins in blue native gels: mitochondrial carriers migrate as monomers not dimers
    Paul G Crichton
    Mitochondrial Biology Unit, Medical Research Council, Hills Road, Cambridge CB2 0XY, United Kingdom
    J Biol Chem 288:22163-73. 2013
    ....
  16. pmc The mimivirus genome encodes a mitochondrial carrier that transports dATP and dTTP
    Magnus Monné
    Dunn Human Nutrition Unit, Medical Research Council, Hills Road, Cambridge CB2 2XY, United Kingdom
    J Virol 81:3181-6. 2007
    ..As the 1.2-Mb double-stranded DNA mimivirus genome is rich in A and T residues, we speculate that the virus is using this protein to target the host mitochondria as a source of deoxynucleotides for its replication...
  17. ncbi request reprint A novel ADP/ATP transporter in the mitosome of the microaerophilic human parasite Entamoeba histolytica
    Ka Wai Chan
    Dunn Human Nutrition Unit, Medical Research Council, Cambridge, UK
    Curr Biol 15:737-42. 2005
    ..Because many functions of yeast and human mitochondria rely on solutes transported by specialized members of this family, the Entamoeba mitosome must contain only a small subset of these processes requiring adenine nucleotide exchange...
  18. doi request reprint Mitochondrial carrier homolog 2 (MTCH2): the recruitment and evolution of a mitochondrial carrier protein to a critical player in apoptosis
    Alan J Robinson
    The Medical Research Council, Mitochondrial Biology Unit, Cambridge, UK
    Exp Cell Res 318:1316-23. 2012
    ....
  19. doi request reprint Coupling of proton and substrate translocation in the transport cycle of mitochondrial carriers
    Edmund R S Kunji
    The Medical Research Council, Mitochondrial Biology Unit, Hills Road, Cambridge CB2 0XY, United Kingdom
    Curr Opin Struct Biol 20:440-7. 2010
    ..In the case of proton-substrate symporters the substrate binding sites contain also negatively charged residues that are proposed to be involved in proton transport...
  20. doi request reprint Structures of yeast mitochondrial ADP/ATP carriers support a domain-based alternating-access transport mechanism
    Jonathan J Ruprecht
    Mitochondrial Biology Unit, Medical Research Council, Cambridge CB2 0XY, United Kingdom
    Proc Natl Acad Sci U S A 111:E426-34. 2014
    ..These findings provide a structural description of transport by mitochondrial carrier proteins, consistent with an alternating-access mechanism. ..
  21. doi request reprint Monitoring the biomass accumulation of recombinant yeast cultures: offline estimations of dry cell mass and cell counts
    Shane M Palmer
    Mitochondrial Biology Unit, The Medical Research Council, Cambridge, UK
    Methods Mol Biol 866:157-63. 2012
    ..In this chapter, protocols are presented for determining these offline measures of the biomass yield of recombinant yeast cultures...
  22. doi request reprint Online monitoring of biomass accumulation in recombinant yeast cultures
    Shane M Palmer
    Mitochondrial Biology Unit, The Medical Research Council, Cambridge, UK
    Methods Mol Biol 866:165-79. 2012
    ..In this chapter, a range of protocols is presented for online determination of biomass yields of recombinant yeast cultures...
  23. doi request reprint Online analysis and process control in recombinant protein production (review)
    Shane M Palmer
    Mitochondrial Biology Unit, The Medical Research Council, Cambridge, UK
    Methods Mol Biol 866:129-55. 2012
    ....
  24. ncbi request reprint Higher plant plastids and cyanobacteria have folate carriers related to those of trypanosomatids
    Sebastian M J Klaus
    Horticultural Sciences, University of Florida, Gainesville, Florida 32611, USA
    J Biol Chem 280:38457-63. 2005
    ..These findings establish conservation of function among folate and biopterin transporter family proteins from three kingdoms of life...
  25. ncbi request reprint Functional and structural role of amino acid residues in the even-numbered transmembrane alpha-helices of the bovine mitochondrial oxoglutarate carrier
    Anna Rita Cappello
    Department of Pharmaco Biology, Laboratory of Biochemistry and Molecular Biology, University of Bari, Via E Orabona 4, 70125 Bari, Italy
    J Mol Biol 363:51-62. 2006
    ..The third cluster is at the midpoint of the membrane and consists of residues that are proposed to be involved in substrate binding...
  26. doi request reprint A novel route for ATP acquisition by the remnant mitochondria of Encephalitozoon cuniculi
    Anastasios D Tsaousis
    Institute for Cell and Molecular Biosciences, Catherine Cookson Building, Framlington Place, Newcastle University, Newcastle upon Tyne NE2 4HH, UK
    Nature 453:553-6. 2008
    ..cuniculi, by reductive evolution and analogous gene replacement. Instead of the mitosome providing the parasite cytosol with ATP, the parasite cytosol now seems to provide ATP for the organelle...
  27. ncbi request reprint Functional and structural role of amino acid residues in the odd-numbered transmembrane alpha-helices of the bovine mitochondrial oxoglutarate carrier
    Anna R Cappello
    Department of Pharmaco Biology, Laboratory of Biochemistry and Molecular Biology, University of Bari, Via E Orabona 4, 70125 Bari, Italy
    J Mol Biol 369:400-12. 2007
    ..Residues G32, A35, Q40, G130, G133, A134, G230, and S237 are potentially engaged in inter-helical interactions and they may be involved in the movements of the alpha-helices during translocation...
  28. pmc Conserved properties of hydrogenosomal and mitochondrial ADP/ATP carriers: a common origin for both organelles
    Mark van der Giezen
    Department of Zoology, The Natural History Museum, Cromwell Road, London SW7 5BD, UK
    EMBO J 21:572-9. 2002
    ..These experiments provide some of the strongest evidence yet that yeast mitochondria and Neocallimastix hydrogenosomes are but two manifestations of the same fundamental organelle...
  29. pmc The insulin-like growth factor-I-mTOR signaling pathway induces the mitochondrial pyrimidine nucleotide carrier to promote cell growth
    Suzanne Floyd
    Cell Biology Laboratory, Department of Biochemistry, Biosciences Institute, National University of Ireland, Cork, Ireland
    Mol Biol Cell 18:3545-55. 2007
    ..Overall the data indicate that PNC1 is a target of the IGF-I/mTOR pathway that is essential for mitochondrial activity in regulating cell growth and proliferation...