Walter Kolch

Summary

Affiliation: University of Glasgow
Country: UK

Publications

  1. ncbi request reprint The Parkinson disease causing LRRK2 mutation I2020T is associated with increased kinase activity
    Christian Johannes Gloeckner
    GSF National Research Center for Environment and Health, Institute of Human Genetics, Munich Neuherberg, Germany
    Hum Mol Genet 15:223-32. 2006
  2. doi request reprint Mutations of beta-arrestin 2 that limit self-association also interfere with interactions with the beta2-adrenoceptor and the ERK1/2 MAPKs: implications for beta2-adrenoceptor signalling via the ERK1/2 MAPKs
    Tian Rui Xu
    Sir Henry Welcome Functional Genomics Facility, Division of Biochemistry and Molecular Biology, Institute of Biomedical and Life Sciences, University of Glasgow, Glasgow G12 8QQ, Scotland, UK
    Biochem J 413:51-60. 2008
  3. pmc Meaningful relationships: the regulation of the Ras/Raf/MEK/ERK pathway by protein interactions
    W Kolch
    The Beatson Institute for Cancer Research, CRC Beatson Laboratories, Garscube Estate, Switchback Road, Bearsden, Glasgow G61 1BD, U K
    Biochem J 351:289-305. 2000
  4. ncbi request reprint Coordinating ERK/MAPK signalling through scaffolds and inhibitors
    Walter Kolch
    Beatson Institute for Cancer Research, Garscube Estate, Switchback Road, Glasgow, G61 1BD, UK
    Nat Rev Mol Cell Biol 6:827-37. 2005
  5. ncbi request reprint When kinases meet mathematics: the systems biology of MAPK signalling
    Walter Kolch
    Sir Henry Wellcome Functional Genomics Facility, Institute of Biomedical and Life Sciences, University of Glasgow, Glasgow G12 8QQ, UK
    FEBS Lett 579:1891-5. 2005
  6. ncbi request reprint The molecular make-up of a tumour: proteomics in cancer research
    Walter Kolch
    Sir Henry Wellcome Functional Genomics Facility, Joseph Black Building, University of Glasgow, Glasgow G12 8QQ, UK
    Clin Sci (Lond) 108:369-83. 2005
  7. ncbi request reprint The role of Raf kinases in malignant transformation
    Walter Kolch
    Institute for Biomedical and Life Sciences, University of Glasgow, University Avenue, Glasgow, G12 8QQ, UK
    Expert Rev Mol Med 4:1-18. 2002
  8. doi request reprint Defining systems biology: through the eyes of a biochemist
    W Kolch
    Beatson Institute for Cancer Research and Institute for Biomedical and Life Sciences, University of Glasgow, Glasgow, UK
    IET Syst Biol 2:5-7. 2008
  9. ncbi request reprint Erbin: sorting out ErbB2 receptors or giving Ras a break?
    Walter Kolch
    Garscube Estate, Cancer Research UK, Beatson Laboratories, Switchback Road, Glasgow G61 1BD, UK
    Sci STKE 2003:pe37. 2003
  10. ncbi request reprint Ras/Raf signalling and emerging pharmacotherapeutic targets
    Walter Kolch
    The Beatson Institute for Cancer Research, Cancer Research UK Beatson Laboratories, Garscube Estate, Glasgow, UK
    Expert Opin Pharmacother 3:709-18. 2002

Detail Information

Publications75

  1. ncbi request reprint The Parkinson disease causing LRRK2 mutation I2020T is associated with increased kinase activity
    Christian Johannes Gloeckner
    GSF National Research Center for Environment and Health, Institute of Human Genetics, Munich Neuherberg, Germany
    Hum Mol Genet 15:223-32. 2006
    ..This suggests that the pathology of PD caused by the I2020T mutation is associated with an increase rather than a loss in LRRK2 kinase activity...
  2. doi request reprint Mutations of beta-arrestin 2 that limit self-association also interfere with interactions with the beta2-adrenoceptor and the ERK1/2 MAPKs: implications for beta2-adrenoceptor signalling via the ERK1/2 MAPKs
    Tian Rui Xu
    Sir Henry Welcome Functional Genomics Facility, Division of Biochemistry and Molecular Biology, Institute of Biomedical and Life Sciences, University of Glasgow, Glasgow G12 8QQ, Scotland, UK
    Biochem J 413:51-60. 2008
    ..Regulation of beta-arrestin 2 self-association may therefore control beta-arrestin 2-mediated beta2-adrenoceptor-ERK1/2 MAPK signalling...
  3. pmc Meaningful relationships: the regulation of the Ras/Raf/MEK/ERK pathway by protein interactions
    W Kolch
    The Beatson Institute for Cancer Research, CRC Beatson Laboratories, Garscube Estate, Switchback Road, Bearsden, Glasgow G61 1BD, U K
    Biochem J 351:289-305. 2000
    ....
  4. ncbi request reprint Coordinating ERK/MAPK signalling through scaffolds and inhibitors
    Walter Kolch
    Beatson Institute for Cancer Research, Garscube Estate, Switchback Road, Glasgow, G61 1BD, UK
    Nat Rev Mol Cell Biol 6:827-37. 2005
    ..Computational modelling indicates that, together, these modulators can determine the dynamic biological behaviour of the pathway...
  5. ncbi request reprint When kinases meet mathematics: the systems biology of MAPK signalling
    Walter Kolch
    Sir Henry Wellcome Functional Genomics Facility, Institute of Biomedical and Life Sciences, University of Glasgow, Glasgow G12 8QQ, UK
    FEBS Lett 579:1891-5. 2005
    ..Systems biology tries to decode this complexity through mathematical modelling and prediction in order to gain a deeper insight into the inner works of signalling networks...
  6. ncbi request reprint The molecular make-up of a tumour: proteomics in cancer research
    Walter Kolch
    Sir Henry Wellcome Functional Genomics Facility, Joseph Black Building, University of Glasgow, Glasgow G12 8QQ, UK
    Clin Sci (Lond) 108:369-83. 2005
    ..A major challenge will be the integration of proteomics with genomics and metabolomics data and their functional interpretation in conjunction with clinical results and epidemiology...
  7. ncbi request reprint The role of Raf kinases in malignant transformation
    Walter Kolch
    Institute for Biomedical and Life Sciences, University of Glasgow, University Avenue, Glasgow, G12 8QQ, UK
    Expert Rev Mol Med 4:1-18. 2002
    ..The molecular mechanisms underlying the role of Raf kinase in tumourigenesis and the opportunities for therapeutic intervention are reviewed in this article...
  8. doi request reprint Defining systems biology: through the eyes of a biochemist
    W Kolch
    Beatson Institute for Cancer Research and Institute for Biomedical and Life Sciences, University of Glasgow, Glasgow, UK
    IET Syst Biol 2:5-7. 2008
  9. ncbi request reprint Erbin: sorting out ErbB2 receptors or giving Ras a break?
    Walter Kolch
    Garscube Estate, Cancer Research UK, Beatson Laboratories, Switchback Road, Glasgow G61 1BD, UK
    Sci STKE 2003:pe37. 2003
    ..It also highlights the complexity of cellular signaling networks in which the tasks of individual components are determined by the specific functional context...
  10. ncbi request reprint Ras/Raf signalling and emerging pharmacotherapeutic targets
    Walter Kolch
    The Beatson Institute for Cancer Research, Cancer Research UK Beatson Laboratories, Garscube Estate, Glasgow, UK
    Expert Opin Pharmacother 3:709-18. 2002
    ..However, it is not clear which components of this pathway should be targeted and how maximum efficacy can be achieved. This paper reviews the current efforts with an emphasis on new mechanistic and conceptual approaches...
  11. ncbi request reprint To be or not to be: a question of B-Raf?
    W Kolch
    Institute for Biomedical and Life Sciences, University of Glasgow, Glasgow, UK
    Trends Neurosci 24:498-500. 2001
    ..This finding sheds new light on the control of neuronal development and poses new questions with regard to Raf-isozyme-specific functions...
  12. pmc A Raf-1 mutant that dissociates MEK/extracellular signal-regulated kinase activation from malignant transformation and differentiation but not proliferation
    Amardeep S Dhillon
    The Beatson Institute for Cancer Research, CR UK Beatson Laboratories, Garscube Estate, Bearsden, Glasgow G61 1BD, Scotland, UK
    Mol Cell Biol 23:1983-93. 2003
    ..The results further suggest that RafS259 mutants inhibit signaling pathways required to promote these biological processes...
  13. doi request reprint The RKIP (Raf-1 Kinase Inhibitor Protein) conserved pocket binds to the phosphorylated N-region of Raf-1 and inhibits the Raf-1-mediated activated phosphorylation of MEK
    Oliver Rath
    The Beatson Institute for Cancer Research, Cancer Research UK Garscube Estate, Bearsden, Glasgow G61 1BD, UK
    Cell Signal 20:935-41. 2008
    ..Our results therefore suggest that the RKIP conserved pocket may constitute a novel phosphoamino-acid binding motif and is absolutely required for RKIP function...
  14. doi request reprint The mammalian MAPK/ERK pathway exhibits properties of a negative feedback amplifier
    Oliver E Sturm
    Department of Computing Science, University of Glasgow, Glasgow G12 8QQ, UK
    Sci Signal 3:ra90. 2010
    ..These properties determine biological behavior, including activation kinetics and the response to drugs...
  15. doi request reprint Inferring signaling pathway topologies from multiple perturbation measurements of specific biochemical species
    Tian Rui Xu
    Faculty of Biomedical and Life Sciences, University of Glasgow, Glasgow G128QQ, UK
    Sci Signal 3:ra20. 2010
    ..Thus, our formal methodology rationally infers evidentially supported pathway topologies even when a limited number of biochemical and kinetic measurements are available...
  16. pmc Regulation and role of Raf-1/B-Raf heterodimerization
    Linda K Rushworth
    Signalling and Proteomics Laboratory, Beatson Institute for Cancer Research, Garscube Estate, Glasgow G61 1BD, United Kingdom
    Mol Cell Biol 26:2262-72. 2006
    ....
  17. pmc Eukaryotic translation initiation factor 3, subunit a, regulates the extracellular signal-regulated kinase pathway
    Tian Rui Xu
    Institute of Neuroscience and Psychology, College of Medical, Veterinary and Life Sciences, University of Glasgow, Glasgow, United Kingdom
    Mol Cell Biol 32:88-95. 2012
    ..Thus, eIF3a is a negative modulator of ERK pathway activation and its biological effects...
  18. ncbi request reprint The kinase domain of MEKK1 induces apoptosis by dysregulation of MAP kinase pathways
    Simone Boldt
    Beatson Institute for Cancer Research, Cancer Research UK, Garscube Estate, Switchback Road, Bearsden, Glasgow G61 1BD, UK
    Exp Cell Res 283:80-90. 2003
    ..This suggests that MEKK1 induces apoptosis by causing a general deregulation of MAP kinase signaling rather than by the activation of a single pathway...
  19. pmc MST kinases monitor actin cytoskeletal integrity and signal via c-Jun N-terminal kinase stress-activated kinase to regulate p21Waf1/Cip1 stability
    Ruth M Densham
    The Beatson Institute for Cancer Research, Garscube Estate, Switchback Road, Glasgow G61 1BD, United Kingdom
    Mol Cell Biol 29:6380-90. 2009
    ....
  20. pmc RASSF1A elicits apoptosis through an MST2 pathway directing proapoptotic transcription by the p73 tumor suppressor protein
    David Matallanas
    Signaling and Proteomics Laboratory, The Beatson Institute for Cancer Research, Garscube Estate, Switchback Road, Bearsden, Glasgow G61 1BD, UK
    Mol Cell 27:962-75. 2007
    ..Our results describe an MST2-dependent effector pathway for RASSF1A proapoptotic signaling and indicate that silencing of RASSF1A in tumors removes a proapoptotic signal emanating from p73...
  21. pmc Regulation of human myoblast differentiation by PEBP4
    Reynaldo Garcia
    Signalling and Proteomics Laboratory, The Beatson Institute for Cancer Research, Garscube Estate, Switchback Road, Bearsden, Glasgow G61 1BD, UK
    EMBO Rep 10:278-84. 2009
    ..Thus, PEBP4 participates in the control of muscle cell differentiation by modulating the activity of MEK and ERK...
  22. ncbi request reprint Regulation of the Raf-MEK-ERK pathway by protein phosphatase 5
    Alex von Kriegsheim
    The Beatson Institute for Cancer Research, Garscube Estate, Switchback Road, Bearsden, Glasgow G61 1BD, UK
    Nat Cell Biol 8:1011-6. 2006
    ..Furthermore, depletion of endogenous PP5 increased cellular phospho-Ser 338 levels. Our results suggest that PP5 is a physiological regulator of Raf-1 signalling pathways...
  23. pmc Extracellular signal-regulated kinase regulates RhoA activation and tumor cell plasticity by inhibiting guanine exchange factor H1 activity
    Anne von Thun
    The Beatson Institute for Cancer Research, Glasgow, United Kingdom
    Mol Cell Biol 33:4526-37. 2013
    ..Thus, we identified GEF-H1 as a critical ERK effector that regulates motility, cell morphology, and invasiveness. ..
  24. ncbi request reprint Taming the Hippo: Raf-1 controls apoptosis by suppressing MST2/Hippo
    Eric O'Neill
    The Beatson Institute for Cancer Research, Glasgow, UK
    Cell Cycle 4:365-7. 2005
    ..Thus, by linking mitogenic and apoptotic signalling the MST:Raf-1 complex may serve as a safeguard against unlicensed proliferation...
  25. ncbi request reprint A Hippo in the ointment: MST signalling beyond the fly
    David Matallanas
    Signalling and Proteomics Laboratory, The Beatson Institute for Cancer Research, Bearsden, Glasgow, United Kingdom
    Cell Cycle 7:879-84. 2008
    ....
  26. doi request reprint The Bcr-Abl kinase regulates the actin cytoskeleton via a GADS/Slp-76/Nck1 adaptor protein pathway
    Christian Preisinger
    Beatson Institute for Cancer Research, Glasgow, UK
    Cell Signal 22:848-56. 2010
    ..These findings highlight the importance of protein interaction dependent adaptor protein pathways in oncogenic kinase signaling...
  27. pmc Cyclic AMP-dependent kinase regulates Raf-1 kinase mainly by phosphorylation of serine 259
    Amardeep S Dhillon
    The Beatson Institute for Cancer Research, Bearsden, Glasgow G61 1BD, United Kingdom
    Mol Cell Biol 22:3237-46. 2002
    ..Using RafS259 mutants we also demonstrate that Raf-1 is the sole target for PKA inhibition of ERK and ERK-induced gene expression, and that Raf-1 inhibition is mediated mainly through serine 259 phosphorylation...
  28. ncbi request reprint Role of the kinase MST2 in suppression of apoptosis by the proto-oncogene product Raf-1
    Eric O'Neill
    The Beatson Institute for Cancer Research, Garscube Estate, Switchback Road, Glasgow G61 1BD, UK
    Science 306:2267-70. 2004
    ..Conversely, depletion of Raf-1 from Raf-1+/+ mouse or human cells led to MST2 activation and apoptosis. The concomitant depletion of both Raf-1 and MST2 prevented apoptosis...
  29. ncbi request reprint Raf kinase inhibitor protein regulation of raf and MAPK signaling
    Suzanne Hagan
    The Beatson Institute for Cancer Research, Signalling and Proteomics Laboratory, Glasgow, United Kingdom
    Methods Enzymol 407:248-59. 2006
    ..More recently, RKIP has emerged as an important suppressor of metastasis. Here, we review the functions of RKIP and present methods to detect and measure RKIP expression and activity in cells and tissues...
  30. ncbi request reprint Untying the regulation of the Raf-1 kinase
    Amardeep S Dhillon
    The Beatson Institute for Cancer Research, Cancer Research UK Beatson Laboratories Garscube Estate, Glasgow, UK
    Arch Biochem Biophys 404:3-9. 2002
    ..KSR is a dynamic scaffold whose function and localization is regulated by phosphorylation...
  31. pmc Computational modelling of cancerous mutations in the EGFR/ERK signalling pathway
    Richard J Orton
    Institute of Comparative Medicine, Faculty of Veterinary Medicine, University of Glasgow, Glasgow, UK
    BMC Syst Biol 3:100. 2009
    ..In this study, we have used computational modelling techniques to investigate what effects various cancerous alterations have on the signalling flow through the ERK pathway...
  32. ncbi request reprint Raf-1 and B-Raf promote protein kinase C theta interaction with BAD
    Alison Hindley
    Signalling and Proteomics Laboratory, The Beatson Institute for Cancer Research, Garscube Estate, Switchback Road, Glasgow G61 1BD, UK
    Cell Signal 19:547-55. 2007
    ....
  33. doi request reprint Proteomics and phosphoproteomics for the mapping of cellular signalling networks
    Christian Preisinger
    The Beatson Institute for Cancer Research, Glasgow, UK
    Proteomics 8:4402-15. 2008
    ....
  34. ncbi request reprint Engineering the serine/threonine protein kinase Raf-1 to utilise an orthogonal analogue of ATP substituted at the N6 position
    Alison D Hindley
    Beatson Institute for Cancer Research, G61 1BD, Glasgow, UK
    FEBS Lett 556:26-34. 2004
    ..Importantly we show that our approach can be used to tag putative direct substrates of Raf-1 kinase with (32)P-N(6)(2-phenethyl) ATP in cell lysates...
  35. doi request reprint Mapping of signaling pathways by functional interaction proteomics
    Alex von Kriegsheim
    Cancer Research Beatson Laboratories, Glasgow, UK
    Methods Mol Biol 484:177-92. 2008
    ..The second is the differential analysis of binding partners of the C-terminal SH3 domain of the two small adaptor proteins Grb2 and GRAP...
  36. pmc Phosphodiesterase-8A binds to and regulates Raf-1 kinase
    Kim M Brown
    Institute of Cardiovascular and Medical Science, Cellular and Systems Biology, College of Medical, Veterinary and Life Sciences, University of Glasgow, Glasgow G12 8QQ, Scotland, United Kingdom
    Proc Natl Acad Sci U S A 110:E1533-42. 2013
    ..Similarly, PDE8 deletion in Drosophila melanogaster reduced basal ERK activation and sensitized flies to stress-induced death. We propose that PDE8A is a physiological regulator of Raf-1 signaling in some cells...
  37. pmc Unique reporter-based sensor platforms to monitor signalling in cells
    Meesbah Jiwaji
    Institute of Molecular, Cell and Systems Biology, College of Medical, Veterinary and Life Sciences, University of Glasgow, Glasgow, United Kingdom
    PLoS ONE 7:e50521. 2012
    ..However, few multiplexed methodologies are available to study cell signalling directly at the transcription factor level...
  38. ncbi request reprint Phosphatase and feedback regulation of Raf-1 signaling
    Amardeep S Dhillon
    Signaling and Proteomics Group, Beatson Institute for Cancer Research, Glasgow, UK
    Cell Cycle 6:3-7. 2007
    ..Both these events require dynamic changes in Raf-1 phosphorylation. Here, we discuss the critical role of phosphatases and feedback phosphorylation during activation and inactivation of Raf-1 signaling...
  39. ncbi request reprint Transcriptional repression of telomerase RNA gene expression by c-Jun-NH2-kinase and Sp1/Sp3
    Alan E Bilsland
    Centre for Oncology and Applied Pharmacology, University of Glasgow, Scotland, UK
    Cancer Res 66:1363-70. 2006
    ..No corresponding changes in Sp1/Sp3 protein levels were detected. Thus, JNK represses hTR promoter activity and expression, apparently by enhancing repression through Sp3...
  40. doi request reprint Chip-based dynamic real-time quantification of drug-induced cytotoxicity in human tumor cells
    Donald Wlodkowic
    The Bioelectronics Research Center, Department of Electronics and Electrical Engineering, University of Glasgow, G12 8LT Glasgow, UK
    Anal Chem 81:6952-9. 2009
    ....
  41. doi request reprint ERK2 drives tumour cell migration in three-dimensional microenvironments by suppressing expression of Rab17 and liprin-β2
    Anne von Thun
    Beatson Institute for Cancer Research, Glasgow, G61 1BD, UK
    J Cell Sci 125:1465-77. 2012
    ..Importantly, knockdown of either Rab17 or liprin-β2 restores invasiveness of ERK2-depleted cells, indicating that ERK2 drives invasion of MDA-MB-231 cells by suppressing expression of these genes...
  42. pmc Cell fate decisions are specified by the dynamic ERK interactome
    Alex von Kriegsheim
    Signalling and Proteomics Laboratory, The Beatson Institute for Cancer Research, Glasgow G61 1BD, UK
    Nat Cell Biol 11:1458-64. 2009
    ..Integrating these data with a mathematical model confirmed that ERK dynamics and differentiation are regulated by distributed control mechanisms rather than by a single master switch...
  43. pmc Regulation of Raf-1 activation and signalling by dephosphorylation
    Amardeep S Dhillon
    The Beatson Institute for Cancer Research, CRC Beatson Laboratories, Garscube Estate, Switchback Road, Bearsden, Glasgow G61 1BD, UK
    EMBO J 21:64-71. 2002
    ..They also suggest a new model for Raf-1 activation where the release of repression through Ser259 dephosphorylation is the pivotal step...
  44. ncbi request reprint The role of MAPK pathways in the action of chemotherapeutic drugs
    Simone Boldt
    The Beatson Institute for Cancer Research, Cancer Research UK, Bearsden, Glasgow G61 1BD, UK
    Carcinogenesis 23:1831-8. 2002
    ..These results suggest that the modulation of MAPK pathways to enhance the efficacy of chemotherapeutic drugs is of limited value unless it is tailored to the specific combination of drug and cancer...
  45. ncbi request reprint Oncogenic K-RAS is required to maintain changes in cytoskeletal organization, adhesion, and motility in colon cancer cells
    Claire B Pollock
    The Beatson Institute for Cancer Research, Garscube Estate, Bearsden, United Kingdom
    Cancer Res 65:1244-50. 2005
    ..Our findings suggest that mutated K-RAS oncogenes are essential for maintenance of the transformed and invasive phenotype of human colon cancer cells...
  46. ncbi request reprint Targeting MAPK signalling: Prometheus' fire or Pandora's box?
    Simone Boldt
    Beatson Institute for Cancer Research, Garscube Estate, Switchback Road, Bearsden Glasgow G61 1BD, UK
    Curr Pharm Des 10:1885-905. 2004
    ..Further, we critically discuss the rationale behind current strategies of using MAPK pathways as drug targets and suggest new approaches that take the complexity of MAPK signalling networks into account...
  47. ncbi request reprint Reduction of Raf-1 kinase inhibitor protein expression correlates with breast cancer metastasis
    Suzanne Hagan
    Beatson Institute for Cancer Research, Signaling and Proteomics Laboratory, Glasgow, UK
    Clin Cancer Res 11:7392-7. 2005
    ..Therefore, we investigated whether RKIP expression is altered in clinical specimens of human primary breast cancers and their lymph node metastases...
  48. ncbi request reprint Oncogenic B-Raf mutations: crystal clear at last
    Amardeep S Dhillon
    The Beatson Institute for Cancer Research, Garscube Estate, Switchback Road, Bearsden, Glasgow G61 1BD, United Kingdom
    Cancer Cell 5:303-4. 2004
    ..A recent study in Cell by Wan et al. reports the crystal structure of the B-Raf kinase domain, providing important new insights into these questions...
  49. pmc Addressing the challenge of defining valid proteomic biomarkers and classifiers
    Mohammed Dakna
    Mosaiques Diagnostics and Therapeutics, Hannover, Germany
    BMC Bioinformatics 11:594. 2010
    ..The second urine of the morning was collected from apparently healthy male and female volunteers (aged 21-40) in the course of the routine medical check-up before recruitment at the Hannover Medical School...
  50. pmc Lab-on-a-chip technologies for proteomic analysis from isolated cells
    H Sedgwick
    Department of Electronics and Electrical Engineering, University of Glasgow, Glasgow G12 8QQ, UK
    J R Soc Interface 5:S123-30. 2008
    ..Using these same trapping electrodes, on-chip electroporation was performed, resulting in cell lysis. Protein release was monitored by confocal fluorescence microscopy...
  51. ncbi request reprint The Raf-1 kinase associates with vimentin kinases and regulates the structure of vimentin filaments
    P Janosch
    The Beatson Institute for Cancer Research, Garscube Estate, Glasgow G61 1BD, U K
    FASEB J 14:2008-21. 2000
    ..The rearrangement of the vimentin network occurred independently of the MEK/ERK pathway. These data identify a new branch point in Raf-1 signaling, which links Raf-1 to changes in the cytoskeletal architecture...
  52. pmc Conferring specificity on the ubiquitous Raf/MEK signalling pathway
    E O'Neill
    Beatson Institute for Cancer Research, Switchback Road, Glasgow G61 1BD, UK
    Br J Cancer 90:283-8. 2004
    ..Here we review how Raf isozyme diversity contributes to the specification of functional diversity, in particular regarding the role of Raf isozymes in cancer...
  53. ncbi request reprint MAP kinase signalling pathways in cancer
    A S Dhillon
    The Beatson Institute for Cancer Research, Bearsden, Glasgow, UK
    Oncogene 26:3279-90. 2007
    ..The balance and integration between these signals may widely vary in different tumours, but are important for the outcome and the sensitivity to drug therapy...
  54. ncbi request reprint Raf kinase inhibitor protein expression combined with peritoneal involvement and lymphovascular invasion predicts prognosis in Dukes' B colorectal cancer patients
    Brendan Doyle
    Beatson Institute for Cancer Research, Glasgow, UK
    Histopathology 62:505-10. 2013
    ..New markers, identifying high-risk Dukes' B patients, are needed. Here, we examine the utility of Raf kinase inhibitor protein (RKIP) as such a marker and promoter methylation as a mechanism of RKIP down-regulation...
  55. doi request reprint Microfluidic single cell arrays to interrogate signalling dynamics of individual, patient-derived hematopoietic stem cells
    Shannon L Faley
    Bioelectronics Research Centre, University of Glasgow, Oakfield Avenue, Glasgow, UK G12 8LT
    Lab Chip 9:2659-64. 2009
    ..Further studies show that dasatinib does, however, markedly reduce CML stem/progenitor cell migration in situ...
  56. doi request reprint On-chip immunoprecipitation for protein purification
    Mairi E Sandison
    Integrative and Systems Biology, FBLS, University of Glasgow, Glasgow, G12 8QQ, UK
    Lab Chip 10:2805-13. 2010
    ..The optimised µIP method was used to obtain purified fractions of affinity-tagged protein from a bacterial lysate...
  57. pmc Computational modelling of the receptor-tyrosine-kinase-activated MAPK pathway
    Richard J Orton
    Bioinformatics Research Centre, Department of Computing Science, University of Glasgow, Glasgow G12 8QQ, Scotland, UK
    Biochem J 392:249-61. 2005
    ..Focusing on the MAPK pathway, we introduce the features and functions of the pathway itself before comparing the available models and describing what new biological insights they have led to...
  58. ncbi request reprint Extracellular signal regulated kinase (ERK)/mitogen activated protein kinase (MAPK)-independent functions of Raf kinases
    Alison Hindley
    The Beatson Institute for Cancer Research, CRC Beatson Laboratories, Glasgow, G61 1BD, UK
    J Cell Sci 115:1575-81. 2002
    ..Other studies have suggested that Raf has kinase-independent functions and may act as a scaffold protein...
  59. ncbi request reprint Mammalian sterile 20-like kinases in tumor suppression: an emerging pathway
    Eric E O'Neill
    Beatson Insitute for Cancer Research, University of Glasgow, Glasgow, United Kingdom
    Cancer Res 65:5485-7. 2005
    ....
  60. pmc The Renilla luciferase gene as a reference gene for normalization of gene expression in transiently transfected cells
    Meesbah Jiwaji
    Institute of Molecular, Cell and Systems Biology, College of Medical, Veterinary and Life Sciences, University of Glasgow, Glasgow, G12 8QQ, UK
    BMC Mol Biol 11:103. 2010
    ..We have developed and validated a normalization method for qPCR using a co-transfected plasmid...
  61. ncbi request reprint Capillary electrophoresis-mass spectrometry as a powerful tool in clinical diagnosis and biomarker discovery
    Walter Kolch
    The Beatson Institute for Cancer Research, Garscube Estate, Switchback Road, Bearsden, Glasgow G61 1BD, United Kingdom
    Mass Spectrom Rev 24:959-77. 2005
    ..Finally, the benefits and limitations of CE-MS for the analysis of proteins in clinical samples are discussed against the background of alternative technologies...
  62. doi request reprint Investigating the correspondence between transcriptomic and proteomic expression profiles using coupled cluster models
    Simon Rogers
    Department of Computing Science, University of Glasgow, Glasgow G128QQ, UK
    Bioinformatics 24:2894-900. 2008
    ..A major open question is whether transcriptome and proteome expression is linked and how it is coordinated...
  63. ncbi request reprint Proteomic analysis of phosphorylation, oxidation and nitrosylation in signal transduction
    Corinne M Spickett
    Department of Bioscience, University of Strathclyde, Glasgow G1 1XQ, UK
    Biochim Biophys Acta 1764:1823-41. 2006
    ....
  64. pmc Regulation of RKIP binding to the N-region of the Raf-1 kinase
    Sungdae Park
    Medical University of Ohio, Department of Biochemistry and Cancer Biology, 3035 Arlington Avenue, Toledo, OH 43614 5804, USA
    FEBS Lett 580:6405-12. 2006
    ....
  65. ncbi request reprint Induction of apoptosis by protein kinase C delta is independent of its kinase activity
    Axel Goerke
    Medical School Hanover, Department of Nephrology, Carl Neuberg Str 1, 30625 Hannover, Germany
    J Biol Chem 277:32054-62. 2002
    ..Our results indicate that distinct protein kinase C isozymes induce apoptosis in vascular smooth muscle cells. More importantly, they show that some protein kinase C effector functions are independent of the catalytic activity...
  66. ncbi request reprint LPS resistance in monocytic cells caused by reverse signaling through transmembrane TNF (mTNF) is mediated by the MAPK/ERK pathway
    Silvia Kirchner
    Department of Hematology, University of Regensburg, Regensburg, Germany
    J Leukoc Biol 75:324-31. 2004
    ..Thus, reverse signaling achieves LPS resistance in monocytic cells through interference with key signal-transduction pathways...
  67. ncbi request reprint High-precision FLIM-FRET in fixed and living cells reveals heterogeneity in a simple CFP-YFP fusion protein
    Michael Millington
    Collaborative Optical Spectroscopy, Micromanipulation and Imaging Centre COSMIC, The University of Edinburgh, King s Buildings, Edinburgh EH9 3JZ, UK
    Biophys Chem 127:155-64. 2007
    ..Our results for this simple control system indicate that the in vivo FLIM-FRET studies of more complex protein-protein interactions would benefit greatly from such quantitative measurements...
  68. ncbi request reprint Reduction in Raf kinase inhibitor protein expression is associated with increased Ras-extracellular signal-regulated kinase signaling in melanoma cell lines
    Marion M Schuierer
    Institute of Pathology, Medical School of the University of Regensburg, Franz Josef Strauss Allee 11, D 93053 Regensburg, Germany
    Cancer Res 64:5186-92. 2004
    ..Taken together, our results suggest that loss of RKIP in malignant melanoma contributes to enhanced invasiveness of transformed cells and therefore to progression of the disease...
  69. ncbi request reprint Mutationally activated K-ras 4A and 4B both mediate lung carcinogenesis
    Charles E Patek
    Sir Alastair Currie Cancer Research UK Laboratories, Molecular Medicine Centre, The University of Edinburgh, Western General Hospital, Edinburgh, UK
    Exp Cell Res 314:1105-14. 2008
    ....
  70. ncbi request reprint Clinical proteomics: a question of technology
    Walter Kolch
    Rapid Commun Mass Spectrom 18:2365-6. 2004
  71. ncbi request reprint Modeling and simulation of intracellular dynamics: choosing an appropriate framework
    Olaf Wolkenhauer
    Systems Biology and Bioinformatics Group, University of Rostock, Rostock 18051, Germany
    IEEE Trans Nanobioscience 3:200-7. 2004
    ....
  72. ncbi request reprint Microarray-formatted clinical biomarker assay development using peptide aptamers to anterior gradient-2
    Euan Murray
    CRUK p53 Signal Transduction Group, Cell Signalling Unit, Institute of Genetics and Molecular Medicine, University of Edinburgh, Edinburgh EH4 2XR, United Kingdom
    Biochemistry 46:13742-51. 2007
    ....
  73. ncbi request reprint Raf kinase inhibitor protein expression in a survival analysis of colorectal cancer patients
    Fahd Al-Mulla
    Kuwait University, Faculty of Medicine, Safat, Kuwait
    J Clin Oncol 24:5672-9. 2006
    ..We examined whether RKIP expression in primary colorectal cancers (CRCs) correlates with the risk of metastasis and overall survival...
  74. ncbi request reprint The International Consortium on Systems Biology of Receptor Tyrosine Kinase Regulatory Networks
    Yoshiyuki Sakaki
    Syst Biol (Stevenage) 152:53-4. 2005
  75. doi request reprint Giving space to cell signaling
    Boris N Kholodenko
    Department of Pathology, Anatomy, and Cell Biology, Thomas Jefferson University, Philadelphia, PA 19107, USA
    Cell 133:566-7. 2008
    ..In this issue, Neves et al. (2008) report how the propagation of spatial information is controlled by the shape of hippocampal neurons and feedback and feed-forward motifs of the beta-adrenergic receptor signaling pathway...