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Genomes and Genes | E Helen KempSummaryAffiliation: University of Sheffield Country: UK Publications
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Publications
Low frequency of pendrin autoantibodies detected using a radioligand binding assay in patients with autoimmune thyroid diseaseE Helen Kemp
Department of Human Metabolism, The Medical School, University of Sheffield, Beech Hill Road, Sheffield S10 2RX, United Kingdom
J Clin Endocrinol Metab 98:E309-13. 2013..These autoantibodies are therefore unlikely to be a useful marker for disease diagnosis, although the role that pendrin may play as an autoantigen in the initiation or maintenance of thyroid autoimmunity remains to be established...
HLA-G does not have a pathophysiological role in Graves' diseaseE H Kemp
Division of Clinical Sciences North, University of Sheffield, UK
J Clin Pathol 56:475-7. 2003..The aim of the current study was to analyse thyroid tissue and thyrocytes obtained from individuals with autoimmune thyroid disease for the expression of HLA-G...
Detection and localization of chemokine gene expression in autoimmune thyroid diseaseE Helen Kemp
Division of Clinical Sciences North, University of Sheffield, Northern General Hospital, UK
Clin Endocrinol (Oxf) 59:207-13. 2003..The aim of this study was to analyse chemokine gene expression in autoimmune thyroid tissue and in cultured thyroid follicular cells (TFC)...- Autoantibodies as diagnostic and predictive markers of vitiligoE Helen Kemp
Division of Clinical Sciences (North, University of Sheffield, Northern General Hospital, Herries Road, Sheffield S5 7AU, UK
Autoimmunity 37:287-90. 2004 - Autoantibody responses to melanocytes in the depigmenting skin disease vitiligoE Helen Kemp
Section of Endocrinology and Reproduction, School of Medicine and Biomedical Sciences, University of Sheffield, Sheffield, UK
Autoimmun Rev 6:138-42. 2007.... - Melanin-concentrating hormone and melanin-concentrating hormone receptors in mammalian skin physiopathologyE Helen Kemp
Department of Human Metabolism, University of Sheffield, United Kingdom
Peptides 30:2071-5. 2009..Recent findings with regard to humoral immune responses to the MCH-R1 in patients with the skin depigmenting disease vitiligo are also discussed... - Mapping of human autoantibody binding sites on the calcium-sensing receptorE Helen Kemp
Department of Human Metabolism, School of Medicine, University of Sheffield, Sheffield S10 2JF, United Kingdom
J Bone Miner Res 25:132-40. 2010..The present work has demonstrated the successful use of phage-display technology in the discovery of CaSR-specific epitopes targeted by human anti-CaSR antibodies... - Activating autoantibodies against the calcium-sensing receptor detected in two patients with autoimmune polyendocrine syndrome type 1E Helen Kemp
Department of Human Metabolism, School of Medicine, University of Sheffield, Royal Hallamshire Hospital, Glossop Road, Sheffield S10 2JF, United Kingdom
J Clin Endocrinol Metab 94:4749-56. 2009..Anti-CaSR binding antibodies have previously been detected in patients with APS1... - Autoantibodies against tyrosine hydroxylase in patients with non-segmental (generalised) vitiligoE Helen Kemp
Department of Human Metabolism, School of Medicine, University of Sheffield, Sheffield, UK
Exp Dermatol 20:35-40. 2011..009). Overall, the results indicate that TH is an antibody target in non-segmental but not in segmental vitiligo and that TH antibodies appear to be more frequent in patients with active vitiligo... - Immunoscreening of phage-displayed cDNA-encoded polypeptides identifies B cell targets in autoimmune diseaseE Helen Kemp
Division of Clinical Sciences North, University of Sheffield, S5 7AU, Sheffield, United Kingdom
Biochem Biophys Res Commun 298:169-77. 2002..Overall, cDNA phage-display technology appears to be applicable to the identification of autoantigens in autoimmune disease... - The melanin-concentrating hormone receptor 1, a novel target of autoantibody responses in vitiligoE Helen Kemp
Division of Clinical Sciences North, University of Sheffield, Sheffield, United Kingdom
J Clin Invest 109:923-30. 2002..Inhibition of MCH binding to its receptor by IgG from vitiligo patients was also shown... - Autoantibodies to tyrosinase-related protein-1 detected in the sera of vitiligo patients using a quantitative radiobinding assayE H Kemp
Section of Medicine, Division of Clinical Sciences Northern General Hospital, University of Sheffield, Sheffield S5 7AU, U K
Br J Dermatol 139:798-805. 1998..The results indicate that autoantibodies to TRP-1 cross-react with tyrosinase and TRP-2, suggesting one or more common epitopes between the three proteins... - Identification of epitopes on tyrosinase which are recognized by autoantibodies from patients with vitiligoE H Kemp
Division of Clinical Sciences, Northern General Hospital, University of Sheffield, U K
J Invest Dermatol 113:267-71. 1999..Thus, the epitopes on tyrosinase recognized by vitiligo patient sera are heterogeneous and include a region with homology to two related proteins which may explain the cross-reactivity previously noted between these antigens... - Analysis of a microsatellite polymorphism of the cytotoxic T-lymphocyte antigen-4 gene in patients with vitiligoE H Kemp
Section of Medicine, Division of Clinical Sciences Northern General Hospital, University of Sheffield, Northern General Hospital, Sheffield S5 7AU, U K
Br J Dermatol 140:73-8. 1999..These results indicate that there is no association between the 106-bp allele and vitiligo, at least when the disorder occurs in the absence of an autoimmune disease... - A cytotoxic T lymphocyte antigen-4 (CTLA-4) gene polymorphism is associated with autoimmune Addison's disease in English patientsE H Kemp
Section of Medicine, Northern General Hospital, University of Sheffield, UK
Clin Endocrinol (Oxf) 49:609-13. 1998..The aim of the present study was to determine whether the same polymorphism of the CTLA-4 gene was associated with autoimmune Addison's disease... - Autoantibodies to the flavoprotein subunit of succinate dehydrogenase: analysis of specificity in autoimmune thyroid diseaseE H Kemp
Division of Clinical Sciences, Northern General Hospital, University of Sheffield, Sheffield, UK
Clin Endocrinol (Oxf) 53:291-9. 2000.... - Autoimmune aspects of vitiligoE H Kemp
Division of Clinical Sciences, Northern General Hospital, University of Sheffield, UK
Autoimmunity 34:65-77. 2001.... - Molecular mapping of epitopes on melanocyte-specific protein Pmel17 which are recognized by autoantibodies in patients with vitiligoE H Kemp
Division of Clinical Sciences, Northern General Hospital, University of Sheffield, Sheffield, UK
Clin Exp Immunol 124:509-15. 2001..It also suggests that the IgG response to Pmel17 is distinct from the antibody response to the other melanocyte-specific antigens... - Identification of antigenic domains on the human sodium-iodide symporter which are recognized by autoantibodies from patients with autoimmune thyroid diseaseE H Kemp
Division of Clinical Sciences, Northern General Hospital, University of Sheffield, Sheffield, UK
Clin Exp Immunol 124:377-85. 2001..No correlation between the recognition of specific epitopes on the human symporter and the type of autoimmune thyroid disease was demonstrated... - Demonstration of autoantibodies against tyrosine hydroxylase in patients with alopecia areataE H Kemp
Department of Human Metabolism, The Medical School, University of Sheffield, Sheffield S10 2RX, UK
Br J Dermatol 165:1236-43. 2011..In addition, melanocyte-specific proteins, gp100 and MelanA, are putative T-cell autoantigens in AA and so may also represent targets of the humoral immune response... - Mapping of melanin-concentrating hormone receptor 1 B cell epitopes predicts two major binding sites for vitiligo patient autoantibodiesNikos G Gavalas
School of Medicine and Biomedical Sciences, University of Sheffield, Sheffield, UK
Exp Dermatol 18:454-63. 2009..Antibodies with MCHR1 function-blocking activity were determined to recognise epitope 254-260, this being the first epitope to be reported as a target site for antibodies that block the function of the receptor... - The calcium-sensing receptor is a target of autoantibodies in patients with autoimmune polyendocrine syndrome type 1Nikos G Gavalas
Section of Endocrinology and Reproduction, School of Medicine and Biomedical Sciences, University of Sheffield, Royal Hallamshire Hospital, Glossop Road, Sheffield, UK
J Clin Endocrinol Metab 92:2107-14. 2007..However, the detection of CaSR antibodies in APS1 remains controversial, with some studies disputing the relevance of the receptor as an autoantigen... - Autoantibodies in vitiligo patients recognize multiple domains of the melanin-concentrating hormone receptorRaju V S R K Gottumukkala
Division of Clinical Sciences North, University of Sheffield, Sheffield, UK
J Invest Dermatol 121:765-70. 2003..Computer prediction of the potential B cell epitopes on melanin-concentrating hormone receptor 1 revealed that the epitope domains identified overlapped, at least in part, with regions predicted to be highly antigenic... - Autoantigens in vitiligo identified by the serological selection of a phage-displayed melanocyte cDNA expression libraryElizabeth A Waterman
Department of Human Metabolism, School of Medicine, University of Sheffield, Sheffield, UK
J Invest Dermatol 130:230-40. 2010..Overall, the study indicated that the targets of autoantibodies in vitiligo patients can be revealed by employing the methodology of phage display... - An insertion/deletion polymorphism in the gene encoding angiotensin converting enzyme is not associated with generalised vitiligo in an English populationSamia Akhtar
Division of Clinical Sciences North, University of Sheffield, Northern General Hospital, Herries Road, Sheffield S5 7AU, UK
Arch Dermatol Res 297:94-8. 2005..42) and that this was also the case for patients with and without associated autoimmunity (P=0.40 and P=0.62, respectively)... - Analysis of allelic variants in the catalase gene in patients with the skin depigmenting disorder vitiligoNikos G Gavalas
Division of Clinical Sciences North, University of Sheffield, UK
Biochem Biophys Res Commun 345:1586-91. 2006.... - The non-synonymous C1858T substitution in the PTPN22 gene is associated with susceptibility to the severe forms of alopecia areataE Helen Kemp
Division of Clinical Sciences North, University of Sheffield, Sheffield, S5 7AU, UK
Hum Immunol 67:535-9. 2006..05). These results suggest that the non-synonymous C1858T substitution in the PTPN22 gene may have an influence on the severity of alopecia areata and provide further evidence for autoimmunity as an aetiological factor in this disorder... - Detection of binding and blocking autoantibodies to the human sodium-iodide symporter in patients with autoimmune thyroid diseaseR A Ajjan
Division of Clinical Sciences, Northern General Hospital, University of Sheffield, United Kingdom
J Clin Endocrinol Metab 85:2020-7. 2000..This system offers the advantage of studying antibody reactivity against conformational epitopes and will be useful in understanding the role of NIS autoreactivity in the pathogenesis of ATD... - Structural insights into autoreactive determinants in thyroid peroxidase composed of discontinuous and multiple key contact amino acid residues contributing to epitopes recognized by patients' autoantibodiesMarlena Dubska
Department of Biochemistry and Molecular Biology, Medical Centre of Postgraduate Education, Marymoncka 99, 01 813 Warsaw, Poland, and Division of Clinical Sciences, North, University of Sheffield, Northern General Hospital, UK
Endocrinology 147:5995-6003. 2006..This identification of key residues within the autoreactive epitopes will help in understanding the structural basis for the breakdown of immune tolerance to TPO in thyroid autoimmune disease... - Real-time detection of stress in 3D tissue-engineered constructs using NF-kappaB activation in transiently transfected human dermal fibroblast cellsIrene Canton
Department of Engineering Materials, Kroto Research Institute, University of Sheffield, and Northern General Hospital, Sheffield, United Kingdom
Tissue Eng 13:1013-24. 2007.... - Autoantibodies in vitiligo patients are not directed to the melanocyte differentiation antigen MelanA/MART1E A Waterman
Division of Clinical Sciences (North, University of Sheffield, UK
Clin Exp Immunol 129:527-32. 2002..It is therefore possible that MelanA only induces cellular rather than humoral autoreactivity in vitiligo... - A single-nucleotide polymorphism in the gene encoding lymphoid protein tyrosine phosphatase (PTPN22) confers susceptibility to generalised vitiligoI Canton
Division of Clinical Sciences North, University of Sheffield, Sheffield, UK
Genes Immun 6:584-7. 2005..17-2.82). The results indicate that the LYP missense R620W polymorphism may have an influence on the development of generalised vitiligo and provide further evidence for autoimmunity as an aetiological factor with respect to this disease... - alpha-Melanocyte-stimulating hormone, MSH 11-13 KPV and adrenocorticotropic hormone signalling in human keratinocyte cellsRichard J Elliott
Department of Engineering Materials, University of Sheffield, Mappin Street, Sheffield, UK
J Invest Dermatol 122:1010-9. 2004..Stable transfection of Chinese hamster ovary cells with the MC-1 receptor showed that alpha-MSH and the KPV peptides elevated intracellular calcium... - Melanoma cell migration is upregulated by tumour necrosis factor-alpha and suppressed by alpha-melanocyte-stimulating hormoneN Zhu
Section of Human Metabolism, Division of Clinical Sciences North, Northern General Hospital, Sheffield S5 7AU, UK
Br J Cancer 90:1457-63. 2004..The data also support a fundamental role of the beta(1)-integrin receptor in melanoma cell migration... - Localization of GerAA and GerAC germination proteins in the Bacillus subtilis sporeK D Hudson
Department of Molecular Biology and Biotechnology, University of Sheffield, Sheffield S10 2TN, United Kingdom
J Bacteriol 183:4317-22. 2001..The results provide support for a model of germination in which, in order to initiate germination, germinant has to permeate the coat and cortex of the spore and bind to a germination receptor located in the inner membrane... - CTLA4 polymorphisms are associated with vitiligo, in patients with concomitant autoimmune diseasesAnne Blomhoff
Institute and Department of Medical Genetics, Ulleval University Hospital, University of Oslo, Oslo, Norway
Pigment Cell Res 18:55-8. 2005..This raises the possibility that there are two distinct forms of vitiligo where only a subgroup of patients may have a disease caused by the autoimmune destruction of melanocytes...