E Helen Kemp

Summary

Affiliation: University of Sheffield
Country: UK

Publications

  1. doi request reprint Low frequency of pendrin autoantibodies detected using a radioligand binding assay in patients with autoimmune thyroid disease
    E Helen Kemp
    Department of Human Metabolism, The Medical School, University of Sheffield, Beech Hill Road, Sheffield S10 2RX, United Kingdom
    J Clin Endocrinol Metab 98:E309-13. 2013
  2. ncbi request reprint Autoantibodies to tyrosinase-related protein-1 detected in the sera of vitiligo patients using a quantitative radiobinding assay
    E H Kemp
    Section of Medicine, Division of Clinical Sciences Northern General Hospital, University of Sheffield, Sheffield S5 7AU, U K
    Br J Dermatol 139:798-805. 1998
  3. pmc HLA-G does not have a pathophysiological role in Graves' disease
    E H Kemp
    Division of Clinical Sciences North, University of Sheffield, UK
    J Clin Pathol 56:475-7. 2003
  4. ncbi request reprint Detection and localization of chemokine gene expression in autoimmune thyroid disease
    E Helen Kemp
    Division of Clinical Sciences North, University of Sheffield, Northern General Hospital, UK
    Clin Endocrinol (Oxf) 59:207-13. 2003
  5. doi request reprint Autoantibodies against tyrosine hydroxylase in patients with non-segmental (generalised) vitiligo
    E Helen Kemp
    Department of Human Metabolism, School of Medicine, University of Sheffield, Sheffield, UK
    Exp Dermatol 20:35-40. 2011
  6. pmc Activating autoantibodies against the calcium-sensing receptor detected in two patients with autoimmune polyendocrine syndrome type 1
    E Helen Kemp
    Department of Human Metabolism, School of Medicine, University of Sheffield, Royal Hallamshire Hospital, Glossop Road, Sheffield S10 2JF, United Kingdom
    J Clin Endocrinol Metab 94:4749-56. 2009
  7. ncbi request reprint Autoantibodies as diagnostic and predictive markers of vitiligo
    E Helen Kemp
    Division of Clinical Sciences North, University of Sheffield, Northern General Hospital, Herries Road, Sheffield S5 7AU, UK
    Autoimmunity 37:287-90. 2004
  8. pmc Mapping of human autoantibody binding sites on the calcium-sensing receptor
    E Helen Kemp
    Department of Human Metabolism, School of Medicine, University of Sheffield, Sheffield S10 2JF, United Kingdom
    J Bone Miner Res 25:132-40. 2010
  9. ncbi request reprint Melanin-concentrating hormone and melanin-concentrating hormone receptors in mammalian skin physiopathology
    E Helen Kemp
    Department of Human Metabolism, University of Sheffield, United Kingdom
    Peptides 30:2071-5. 2009
  10. ncbi request reprint Autoantibody responses to melanocytes in the depigmenting skin disease vitiligo
    E Helen Kemp
    Section of Endocrinology and Reproduction, School of Medicine and Biomedical Sciences, University of Sheffield, Sheffield, UK
    Autoimmun Rev 6:138-42. 2007

Collaborators

Detail Information

Publications38

  1. doi request reprint Low frequency of pendrin autoantibodies detected using a radioligand binding assay in patients with autoimmune thyroid disease
    E Helen Kemp
    Department of Human Metabolism, The Medical School, University of Sheffield, Beech Hill Road, Sheffield S10 2RX, United Kingdom
    J Clin Endocrinol Metab 98:E309-13. 2013
    ..However, a subsequent study failed to verify this in autoimmune thyroid disease (ATD) patients of Tunisian origin...
  2. ncbi request reprint Autoantibodies to tyrosinase-related protein-1 detected in the sera of vitiligo patients using a quantitative radiobinding assay
    E H Kemp
    Section of Medicine, Division of Clinical Sciences Northern General Hospital, University of Sheffield, Sheffield S5 7AU, U K
    Br J Dermatol 139:798-805. 1998
    ..The results indicate that autoantibodies to TRP-1 cross-react with tyrosinase and TRP-2, suggesting one or more common epitopes between the three proteins...
  3. pmc HLA-G does not have a pathophysiological role in Graves' disease
    E H Kemp
    Division of Clinical Sciences North, University of Sheffield, UK
    J Clin Pathol 56:475-7. 2003
    ..The aim of the current study was to analyse thyroid tissue and thyrocytes obtained from individuals with autoimmune thyroid disease for the expression of HLA-G...
  4. ncbi request reprint Detection and localization of chemokine gene expression in autoimmune thyroid disease
    E Helen Kemp
    Division of Clinical Sciences North, University of Sheffield, Northern General Hospital, UK
    Clin Endocrinol (Oxf) 59:207-13. 2003
    ..The aim of this study was to analyse chemokine gene expression in autoimmune thyroid tissue and in cultured thyroid follicular cells (TFC)...
  5. doi request reprint Autoantibodies against tyrosine hydroxylase in patients with non-segmental (generalised) vitiligo
    E Helen Kemp
    Department of Human Metabolism, School of Medicine, University of Sheffield, Sheffield, UK
    Exp Dermatol 20:35-40. 2011
    ..009). Overall, the results indicate that TH is an antibody target in non-segmental but not in segmental vitiligo and that TH antibodies appear to be more frequent in patients with active vitiligo...
  6. pmc Activating autoantibodies against the calcium-sensing receptor detected in two patients with autoimmune polyendocrine syndrome type 1
    E Helen Kemp
    Department of Human Metabolism, School of Medicine, University of Sheffield, Royal Hallamshire Hospital, Glossop Road, Sheffield S10 2JF, United Kingdom
    J Clin Endocrinol Metab 94:4749-56. 2009
    ..Anti-CaSR binding antibodies have previously been detected in patients with APS1...
  7. ncbi request reprint Autoantibodies as diagnostic and predictive markers of vitiligo
    E Helen Kemp
    Division of Clinical Sciences North, University of Sheffield, Northern General Hospital, Herries Road, Sheffield S5 7AU, UK
    Autoimmunity 37:287-90. 2004
  8. pmc Mapping of human autoantibody binding sites on the calcium-sensing receptor
    E Helen Kemp
    Department of Human Metabolism, School of Medicine, University of Sheffield, Sheffield S10 2JF, United Kingdom
    J Bone Miner Res 25:132-40. 2010
    ..The present work has demonstrated the successful use of phage-display technology in the discovery of CaSR-specific epitopes targeted by human anti-CaSR antibodies...
  9. ncbi request reprint Melanin-concentrating hormone and melanin-concentrating hormone receptors in mammalian skin physiopathology
    E Helen Kemp
    Department of Human Metabolism, University of Sheffield, United Kingdom
    Peptides 30:2071-5. 2009
    ..Recent findings with regard to humoral immune responses to the MCH-R1 in patients with the skin depigmenting disease vitiligo are also discussed...
  10. ncbi request reprint Autoantibody responses to melanocytes in the depigmenting skin disease vitiligo
    E Helen Kemp
    Section of Endocrinology and Reproduction, School of Medicine and Biomedical Sciences, University of Sheffield, Sheffield, UK
    Autoimmun Rev 6:138-42. 2007
    ....
  11. ncbi request reprint Immunoscreening of phage-displayed cDNA-encoded polypeptides identifies B cell targets in autoimmune disease
    E Helen Kemp
    Division of Clinical Sciences North, University of Sheffield, S5 7AU, Sheffield, United Kingdom
    Biochem Biophys Res Commun 298:169-77. 2002
    ..Overall, cDNA phage-display technology appears to be applicable to the identification of autoantigens in autoimmune disease...
  12. pmc The melanin-concentrating hormone receptor 1, a novel target of autoantibody responses in vitiligo
    E Helen Kemp
    Division of Clinical Sciences North, University of Sheffield, Sheffield, United Kingdom
    J Clin Invest 109:923-30. 2002
    ..Inhibition of MCH binding to its receptor by IgG from vitiligo patients was also shown...
  13. ncbi request reprint Autoimmune aspects of vitiligo
    E H Kemp
    Division of Clinical Sciences, Northern General Hospital, University of Sheffield, UK
    Autoimmunity 34:65-77. 2001
    ....
  14. pmc Molecular mapping of epitopes on melanocyte-specific protein Pmel17 which are recognized by autoantibodies in patients with vitiligo
    E H Kemp
    Division of Clinical Sciences, Northern General Hospital, University of Sheffield, Sheffield, UK
    Clin Exp Immunol 124:509-15. 2001
    ..It also suggests that the IgG response to Pmel17 is distinct from the antibody response to the other melanocyte-specific antigens...
  15. ncbi request reprint A cytotoxic T lymphocyte antigen-4 (CTLA-4) gene polymorphism is associated with autoimmune Addison's disease in English patients
    E H Kemp
    Section of Medicine, Northern General Hospital, University of Sheffield, UK
    Clin Endocrinol (Oxf) 49:609-13. 1998
    ..The aim of the present study was to determine whether the same polymorphism of the CTLA-4 gene was associated with autoimmune Addison's disease...
  16. pmc Identification of antigenic domains on the human sodium-iodide symporter which are recognized by autoantibodies from patients with autoimmune thyroid disease
    E H Kemp
    Division of Clinical Sciences, Northern General Hospital, University of Sheffield, Sheffield, UK
    Clin Exp Immunol 124:377-85. 2001
    ..No correlation between the recognition of specific epitopes on the human symporter and the type of autoimmune thyroid disease was demonstrated...
  17. ncbi request reprint Autoantibodies to the flavoprotein subunit of succinate dehydrogenase: analysis of specificity in autoimmune thyroid disease
    E H Kemp
    Division of Clinical Sciences, Northern General Hospital, University of Sheffield, Sheffield, UK
    Clin Endocrinol (Oxf) 53:291-9. 2000
    ....
  18. ncbi request reprint Analysis of a microsatellite polymorphism of the cytotoxic T-lymphocyte antigen-4 gene in patients with vitiligo
    E H Kemp
    Section of Medicine, Division of Clinical Sciences Northern General Hospital, University of Sheffield, Northern General Hospital, Sheffield S5 7AU, U K
    Br J Dermatol 140:73-8. 1999
    ..These results indicate that there is no association between the 106-bp allele and vitiligo, at least when the disorder occurs in the absence of an autoimmune disease...
  19. ncbi request reprint Identification of epitopes on tyrosinase which are recognized by autoantibodies from patients with vitiligo
    E H Kemp
    Division of Clinical Sciences, Northern General Hospital, University of Sheffield, U K
    J Invest Dermatol 113:267-71. 1999
    ..Thus, the epitopes on tyrosinase recognized by vitiligo patient sera are heterogeneous and include a region with homology to two related proteins which may explain the cross-reactivity previously noted between these antigens...
  20. doi request reprint Demonstration of autoantibodies against tyrosine hydroxylase in patients with alopecia areata
    E H Kemp
    Department of Human Metabolism, The Medical School, University of Sheffield, Sheffield S10 2RX, UK
    Br J Dermatol 165:1236-43. 2011
    ..In addition, melanocyte-specific proteins, gp100 and MelanA, are putative T-cell autoantigens in AA and so may also represent targets of the humoral immune response...
  21. doi request reprint Mapping of melanin-concentrating hormone receptor 1 B cell epitopes predicts two major binding sites for vitiligo patient autoantibodies
    Nikos G Gavalas
    School of Medicine and Biomedical Sciences, University of Sheffield, Sheffield, UK
    Exp Dermatol 18:454-63. 2009
    ..Antibodies with MCHR1 function-blocking activity were determined to recognise epitope 254-260, this being the first epitope to be reported as a target site for antibodies that block the function of the receptor...
  22. ncbi request reprint The calcium-sensing receptor is a target of autoantibodies in patients with autoimmune polyendocrine syndrome type 1
    Nikos G Gavalas
    Section of Endocrinology and Reproduction, School of Medicine and Biomedical Sciences, University of Sheffield, Royal Hallamshire Hospital, Glossop Road, Sheffield, UK
    J Clin Endocrinol Metab 92:2107-14. 2007
    ..However, the detection of CaSR antibodies in APS1 remains controversial, with some studies disputing the relevance of the receptor as an autoantigen...
  23. ncbi request reprint Autoantibodies in vitiligo patients recognize multiple domains of the melanin-concentrating hormone receptor
    Raju V S R K Gottumukkala
    Division of Clinical Sciences North, University of Sheffield, Sheffield, UK
    J Invest Dermatol 121:765-70. 2003
    ..Computer prediction of the potential B cell epitopes on melanin-concentrating hormone receptor 1 revealed that the epitope domains identified overlapped, at least in part, with regions predicted to be highly antigenic...
  24. ncbi request reprint An insertion/deletion polymorphism in the gene encoding angiotensin converting enzyme is not associated with generalised vitiligo in an English population
    Samia Akhtar
    Division of Clinical Sciences North, University of Sheffield, Northern General Hospital, Herries Road, Sheffield S5 7AU, UK
    Arch Dermatol Res 297:94-8. 2005
    ..42) and that this was also the case for patients with and without associated autoimmunity (P=0.40 and P=0.62, respectively)...
  25. ncbi request reprint Analysis of allelic variants in the catalase gene in patients with the skin depigmenting disorder vitiligo
    Nikos G Gavalas
    Division of Clinical Sciences North, University of Sheffield, UK
    Biochem Biophys Res Commun 345:1586-91. 2006
    ....
  26. doi request reprint Autoantigens in vitiligo identified by the serological selection of a phage-displayed melanocyte cDNA expression library
    Elizabeth A Waterman
    Department of Human Metabolism, School of Medicine, University of Sheffield, Sheffield, UK
    J Invest Dermatol 130:230-40. 2010
    ..Overall, the study indicated that the targets of autoantibodies in vitiligo patients can be revealed by employing the methodology of phage display...
  27. ncbi request reprint The non-synonymous C1858T substitution in the PTPN22 gene is associated with susceptibility to the severe forms of alopecia areata
    E Helen Kemp
    Division of Clinical Sciences North, University of Sheffield, Sheffield, S5 7AU, UK
    Hum Immunol 67:535-9. 2006
    ..05). These results suggest that the non-synonymous C1858T substitution in the PTPN22 gene may have an influence on the severity of alopecia areata and provide further evidence for autoimmunity as an aetiological factor in this disorder...
  28. ncbi request reprint Detection of binding and blocking autoantibodies to the human sodium-iodide symporter in patients with autoimmune thyroid disease
    R A Ajjan
    Division of Clinical Sciences, Northern General Hospital, University of Sheffield, United Kingdom
    J Clin Endocrinol Metab 85:2020-7. 2000
    ..This system offers the advantage of studying antibody reactivity against conformational epitopes and will be useful in understanding the role of NIS autoreactivity in the pathogenesis of ATD...
  29. ncbi request reprint Prevalence and clinical associations of calcium-sensing receptor and NALP5 autoantibodies in Finnish APECED patients
    E Helen Kemp
    Department of Human Metabolism E H K, M H, H K S, A P W, The Medical School, University of Sheffield, Sheffield S10 2RX, United Kingdom Department of Dermatology, Allergology, and Venereology N K, A R, Institute of Clinical Medicine, University of Helsinki and Helsinki University Central Hospital, 00290 Helsinki, Finland and Clinical Research Institute K J E K, HUCH Ltd, 00029 HUS Helsinki, Finland
    J Clin Endocrinol Metab 99:1064-71. 2014
    ..However, although NALP5 antibodies have been associated with the occurrence of hypoparathyroidism (HP) in APECED, it is unclear whether CaSR antibodies are a specific or sensitive marker for APECED-associated HP...
  30. ncbi request reprint Structural insights into autoreactive determinants in thyroid peroxidase composed of discontinuous and multiple key contact amino acid residues contributing to epitopes recognized by patients' autoantibodies
    Marlena Dubska
    Department of Biochemistry and Molecular Biology, Medical Centre of Postgraduate Education, Marymoncka 99, 01 813 Warsaw, Poland, and Division of Clinical Sciences, North, University of Sheffield, Northern General Hospital, UK
    Endocrinology 147:5995-6003. 2006
    ..This identification of key residues within the autoreactive epitopes will help in understanding the structural basis for the breakdown of immune tolerance to TPO in thyroid autoimmune disease...
  31. ncbi request reprint Real-time detection of stress in 3D tissue-engineered constructs using NF-kappaB activation in transiently transfected human dermal fibroblast cells
    Irene Canton
    Department of Engineering Materials, Kroto Research Institute, University of Sheffield, and Northern General Hospital, Sheffield, United Kingdom
    Tissue Eng 13:1013-24. 2007
    ....
  32. pmc Autoantibodies in vitiligo patients are not directed to the melanocyte differentiation antigen MelanA/MART1
    E A Waterman
    Division of Clinical Sciences North, University of Sheffield, UK
    Clin Exp Immunol 129:527-32. 2002
    ..It is therefore possible that MelanA only induces cellular rather than humoral autoreactivity in vitiligo...
  33. ncbi request reprint A single-nucleotide polymorphism in the gene encoding lymphoid protein tyrosine phosphatase (PTPN22) confers susceptibility to generalised vitiligo
    I Canton
    Division of Clinical Sciences North, University of Sheffield, Sheffield, UK
    Genes Immun 6:584-7. 2005
    ..17-2.82). The results indicate that the LYP missense R620W polymorphism may have an influence on the development of generalised vitiligo and provide further evidence for autoimmunity as an aetiological factor with respect to this disease...
  34. doi request reprint Melanocyte antigen-specific antibodies cannot be used as markers for recent disease activity in patients with vitiligo
    M W Kroon
    Department of Dermatology, Netherlands Institute for Pigment Disorders, Academic Medical Center, University of Amsterdam, Amsterdam, The Netherlands
    J Eur Acad Dermatol Venereol 27:1172-5. 2013
    ..Melanocyte antigen-specific antibodies are frequently found in the sera of patients with vitiligo and the presence of these antibodies may correlate with disease activity...
  35. ncbi request reprint alpha-Melanocyte-stimulating hormone, MSH 11-13 KPV and adrenocorticotropic hormone signalling in human keratinocyte cells
    Richard J Elliott
    Department of Engineering Materials, University of Sheffield, Mappin Street, Sheffield, UK
    J Invest Dermatol 122:1010-9. 2004
    ..Stable transfection of Chinese hamster ovary cells with the MC-1 receptor showed that alpha-MSH and the KPV peptides elevated intracellular calcium...
  36. pmc Melanoma cell migration is upregulated by tumour necrosis factor-alpha and suppressed by alpha-melanocyte-stimulating hormone
    N Zhu
    Section of Human Metabolism, Division of Clinical Sciences North, Northern General Hospital, Sheffield S5 7AU, UK
    Br J Cancer 90:1457-63. 2004
    ..The data also support a fundamental role of the beta(1)-integrin receptor in melanoma cell migration...
  37. pmc Localization of GerAA and GerAC germination proteins in the Bacillus subtilis spore
    K D Hudson
    Department of Molecular Biology and Biotechnology, University of Sheffield, Sheffield S10 2TN, United Kingdom
    J Bacteriol 183:4317-22. 2001
    ..The results provide support for a model of germination in which, in order to initiate germination, germinant has to permeate the coat and cortex of the spore and bind to a germination receptor located in the inner membrane...
  38. ncbi request reprint CTLA4 polymorphisms are associated with vitiligo, in patients with concomitant autoimmune diseases
    Anne Blomhoff
    Institute and Department of Medical Genetics, Ulleval University Hospital, University of Oslo, Oslo, Norway
    Pigment Cell Res 18:55-8. 2005
    ..This raises the possibility that there are two distinct forms of vitiligo where only a subgroup of patients may have a disease caused by the autoimmune destruction of melanocytes...