Research Topics
| Douglas B KellSummaryAffiliation: University of Manchester Country: UK Publications
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Detail Information
Publications
The promiscuous binding of pharmaceutical drugs and their transporter-mediated uptake into cells: what we (need to) know and how we can do soDouglas B Kell
School of Chemistry, The University of Manchester, 131 Princess St, Manchester M1 7DN, UK
Drug Discov Today 18:218-39. 2013..This needs to be acquired in cells that contain the relevant proteins, and we highlight an experimental system for simultaneous genome-wide assessment of carrier-mediated uptake in a eukaryotic cell (yeast)...
The genetic control of growth rate: a systems biology study in yeastPinar Pir
Cambridge Systems Biology Centre and Department of Biochemistry, University of Cambridge, Sanger Building, 80 Tennis Court Road, Cambridge CB2 1GA, UK
BMC Syst Biol 6:4. 2012..It has not been determined whether the set of HFC genes is the same at all growth rates or whether it is the same in conditions of nutrient limitation or excess...
Large-scale sequestration of atmospheric carbon via plant roots in natural and agricultural ecosystems: why and howDouglas B Kell
School of Chemistry and Manchester Interdisciplinary Biocentre, University of Manchester, 131 Princess St, Manchester M1 7DN, UK
Philos Trans R Soc Lond B Biol Sci 367:1589-97. 2012..These bring additional benefits related to improvements in soil structure and in the usage of other nutrients and water...
Scientific discovery as a combinatorial optimisation problem: how best to navigate the landscape of possible experiments?Douglas B Kell
School of Chemistry and Manchester Interdisciplinary Biocentre, The University of Manchester, Manchester, Lancs, UK
Bioessays 34:236-44. 2012..This analysis fits comfortably with an emerging epistemology that sees scientific reasoning, the search for solutions, and scientific discovery as Bayesian processes...
Interactions among oscillatory pathways in NF-kappa B signalingYunjiao Wang
Mathematical Biosciences Institute, The Ohio State University, Jennings Hall, Columbus, Ohio 43210, USA
BMC Syst Biol 5:23. 2011..Many authors have suggested that this core oscillator should couple to other oscillatory pathways...
The markup is the model: reasoning about systems biology models in the Semantic Web eraDouglas B Kell
School of Chemistry, The University of Manchester, 131 Princess Street, Manchester M1 7DN, UK
J Theor Biol 252:538-43. 2008..Optimization is at the core of many scientific and biotechnological activities, and Reinhart made many major contributions in this area, stimulating our own activities in the use of the methods of evolutionary computing for optimization...
Metabolomics, machine learning and modelling: towards an understanding of the language of cellsD B Kell
School of Chemistry, The University of Manchester, Faraday Building, Sackville Street, P O Box 88, Manchester M60 1QD, UK
Biochem Soc Trans 33:520-4. 2005..The language of cells is much richer than we had supposed, and we are now well placed to decode it...
Towards a unifying, systems biology understanding of large-scale cellular death and destruction caused by poorly liganded iron: Parkinson's, Huntington's, Alzheimer's, prions, bactericides, chemical toxicology and others as examplesDouglas B Kell
School of Chemistry and The Manchester Interdisciplinary Biocentre, The University of Manchester, Manchester M1 7DN, UK
Arch Toxicol 84:825-89. 2010..A variety of systems biology approaches, that I summarise, can predict both the mechanisms involved in these cell death pathways and the optimal sites of action for nutritional or pharmacological interventions...
The virtual human: towards a global systems biology of multiscale, distributed biochemical network modelsDouglas B Kell
School of Chemistry and The Manchester Centre for Integrative Systems Biology, The Manchester Interdisciplinary Biocentre, The University of Manchester, Manchester, UK
IUBMB Life 59:689-95. 2007..This kind of architecture permits the distributed yet integrated goal of an evolving 'digital human' model to be realized...
Iron behaving badly: inappropriate iron chelation as a major contributor to the aetiology of vascular and other progressive inflammatory and degenerative diseasesDouglas B Kell
School of Chemistry and Manchester Interdisciplinary Biocentre, The University of Manchester, 131 Princess St, Manchester, M1 7DN, UK
BMC Med Genomics 2:2. 2009..Understanding these requires an integrative, systems-level approach that may lead to novel therapeutic targets...
Pharmaceutical drug transport: the issues and the implications that it is essentially carrier-mediated onlyDouglas B Kell
School of Chemistry, The University of Manchester, 131 Princess St, Manchester, M1 7DN, UK
Drug Discov Today 16:704-14. 2011..The recognition that drugs necessarily require carriers for uptake into cells provides many opportunities for improving the effectiveness of the drug discovery process...
Systems biology, metabolic modelling and metabolomics in drug discovery and developmentDouglas B Kell
School of Chemistry, Faraday Building, The University of Manchester PO Box 88, Manchester, M60 1QD, UK
Drug Discov Today 11:1085-92. 2006....
Breeding crop plants with deep roots: their role in sustainable carbon, nutrient and water sequestrationDouglas B Kell
School of Chemistry and Manchester Interdisciplinary Biocentre, University of Manchester, Manchester M1 7DN, UK
Ann Bot 108:407-18. 2011..This sets an important research agenda, and the breeding of plants with improved and deep rooting habits and architectures is a goal well worth pursuing...
Theodor Bücher Lecture. Metabolomics, modelling and machine learning in systems biology - towards an understanding of the languages of cells. Delivered on 3 July 2005 at the 30th FEBS Congress and the 9th IUBMB conference in BudapestDouglas B Kell
School of Chemistry, Faraday Building, The University of Manchester, UK
FEBS J 273:873-94. 2006..These include fields such as chemical genomics, synthetic biology, distributed computational environments for biological data and modelling, single cell diagnostics/bionanotechnology, and computational linguistics/text mining...
Metabolic footprinting and systems biology: the medium is the messageDouglas B Kell
School of Chemistry, University of Manchester, Faraday Building, PO Box 88, Sackville Street, Manchester M60 1QD, UK
Nat Rev Microbiol 3:557-65. 2005..Here, we review the principles, experimental approaches and scientific outcomes that have been obtained with this useful and convenient strategy...
Genome-wide assessment of the carriers involved in the cellular uptake of drugs: a model system in yeastKarin Lanthaler
School of Chemistry, University of Manchester, Manchester, UK
BMC Biol 9:70. 2011..The recent recognition that drug uptake is mostly carrier-mediated raises the question of which drugs use which carriers...
Absolute quantification of the glycolytic pathway in yeast: deployment of a complete QconCAT approachKathleen M Carroll
Manchester Centre for Integrative Systems Biology, Manchester Interdisciplinary Biocentre, University of Manchester, Manchester, United Kingdom
Mol Cell Proteomics 10:M111.007633. 2011..In addition, the same proteins were quantified by intensity-based label-free analysis, and both sets of data were compared with other quantification methods...
MeMo: a hybrid SQL/XML approach to metabolomic data management for functional genomicsIrena Spasic
School of Chemistry, Faraday Building, The University of Manchester, Manchester, M60 1QD, UK
BMC Bioinformatics 7:281. 2006..The metabolomic data produced need to be structured and annotated in a machine-usable form to facilitate the exploration of the hidden links between the genes and their functions...
Calling International Rescue: knowledge lost in literature and data landslide!Teresa K Attwood
School of Chemistry, The University of Manchester, Oxford Road, Manchester M13 9PL, UK
Biochem J 424:317-33. 2009..We ask you, please, to read the instructions carefully. The time has come: you may turn over your papers.....
Bayesian inference of the sites of perturbations in metabolic pathways via Markov chain Monte CarloBayu Jayawardhana
Manchester Interdisciplinary Biocentre, The University of Manchester, Manchester M1 7DN, UK
Bioinformatics 24:1191-7. 2008..In this regard, the ability to identify target and off-target effects of a specific compound or gene therapy is both a major challenge and critical in drug discovery...
Facilitating the development of controlled vocabularies for metabolomics technologies with text miningIrena Spasic
Manchester Centre for Integrative Systems Biology, The University of Manchester, 131 Princess Street, Manchester, M1 7ND, UK
BMC Bioinformatics 9:S5. 2008..However, it is time-consuming and non trivial to construct these resources manually...
Information management for high content live cell imagingDaniel Jameson
Manchester Centre for Integrative Systems Biology, School of Chemistry, and Manchester Interdisciplinary Biocentre, University of Manchester, 131, Princess St, Manchester, M1 7DN, UK
BMC Bioinformatics 10:226. 2009..There is therefore a requirement for an information management solution that facilitates the indexing of experimental metadata and results of high content live cell imaging experiments...
Performing statistical analyses on quantitative data in Taverna workflows: an example using R and maxdBrowse to identify differentially-expressed genes from microarray dataPeter Li
Manchester Centre for Integrative Systems Biology and School of Chemistry, Manchester Interdisciplinary Biocentre, University of Manchester, 131 Princess St, Manchester, M1 7DN, UK
BMC Bioinformatics 9:334. 2008..Furthermore, this transfer of data may require a reconciliation step in order for there to be interoperability between computational tools...
Improving metabolic flux predictions using absolute gene expression dataDAVE LEE
Manchester Institute of Biotechnology, University of Manchester, 131 Princess Street, Manchester, M1 7DN, UK
BMC Syst Biol 6:73. 2012..Moreover, for the purposes of biotechnological applications, it is normally the flux to a specific metabolite or product that is of interest rather than the rate of production of biomass per se...
Further developments towards a genome-scale metabolic model of yeastPaul D Dobson
School of Chemistry, The University of Manchester, Manchester M13 9PL, UK
BMC Syst Biol 4:145. 2010....
Detection and identification of novel metabolomic biomarkers in preeclampsiaLouise C Kenny
Maternal and Fetal Health Research Centre, University of Manchester, St Mary s Hospital, Manchester, United Kingdom
Reprod Sci 15:591-7. 2008..The identity of these metabolites provides new insights into the pathology of this condition and raises the possibility of the development of a predictive test...
Automated manipulation of systems biology models using libSBML within Taverna workflowsPeter Li
School of Chemistry and Manchester Centre for Integrative Systems Biology, Manchester Interdisciplinary Biocentre, University of Manchester, UK
Bioinformatics 24:287-9. 2008..AVAILABILITY: Taverna and the API Consumer application can be freely downloaded from http://taverna.sourceforge.net..
Analysis of a complete DNA-protein affinity landscapeWilliam Rowe
Manchester Interdisciplinary Biocentre, University of Manchester, 131 Princess Street, Manchester M1 7DN, UK
J R Soc Interface 7:397-408. 2010..These metrics show that the landscape is rugged, with many local optima, and that this arises from a combination of experimental variation and the natural structural properties of the oligonucleotides...
Procedures for large-scale metabolic profiling of serum and plasma using gas chromatography and liquid chromatography coupled to mass spectrometryWarwick B Dunn
Manchester Centre for Integrative Systems Biology, Manchester Interdisciplinary Biocentre, University of Manchester, Manchester, UK
Nat Protoc 6:1060-83. 2011..Methods for quality control-based robust LOESS signal correction to provide signal correction and integration of data from multiple analytical batches are also described...
Metabolomics by numbers: acquiring and understanding global metabolite dataRoyston Goodacre
Department of Chemistry, UMIST, P.O. Box 88, Sackville Street, Manchester M60 1QD, UK
Trends Biotechnol 22:245-52. 2004
Enzyme kinetics informatics: from instrument to browserNeil Swainston
Manchester Centre for Integrative Systems Biology, University of Manchester, Manchester, UK
FEBS J 277:3769-79. 2010..The system is designed to be extensible, allowing integration with other manufacturer instruments covering a range of analytical techniques...
Predictive models for population performance on real biological fitness landscapesWilliam Rowe
Faculty of Life Sciences, The University of Manchester, Michael Smith Building, UK
Bioinformatics 26:2145-52. 2010..A modification to the standard LSM also proves accurate at predicting the effects of recombination on the evolution...
In silico modelling of directed evolution: Implications for experimental design and stepwise evolutionDavid C Wedge
Manchester Interdisciplinary Biocentre, University of Manchester, 131 Princess Street, Manchester, M1 7ND, UK
J Theor Biol 257:131-41. 2009..Overall, we find that purely evolutionary techniques fare better than do model-based approaches across all but the smoothest landscapes...
A systematic approach to modeling, capturing, and disseminating proteomics experimental dataChris F Taylor
School of Biological Sciences, University of Manchester, Oxford Road, Manchester M13 9PL, UK
Nat Biotechnol 21:247-54. 2003..These make explicit what data might be most usefully captured about proteomics experiments and provide complementary routes toward the implementation of a proteome repository...
Optimal construction of a fast and accurate polarisable water potential based on multipole moments trained by machine learningChris M Handley
Manchester Interdisciplinary Biocentre, 131 Princess Street, Manchester, UK
Phys Chem Chem Phys 11:6365-76. 2009..Compared to the Kriging results, combinations are found that are no less accurate (at the 90th energy error percentile), yet are 58% faster for the dimer, and 26% faster for the pentamer...
A GC-TOF-MS study of the stability of serum and urine metabolomes during the UK Biobank sample collection and preparation protocolsWarwick B Dunn
Bioanalytical Sciences Group, School of Chemistry, Manchester Interdiscplinary Biocentre, University of Manchester, 131 Princess Street, Manchester, M1 7ND, UK
Int J Epidemiol 37:i23-30. 2008....
Automated workflows for accurate mass-based putative metabolite identification in LC/MS-derived metabolomic datasetsMarie Brown
School of Biomedicine, The University of Manchester, Manchester M13 9PT, UK
Bioinformatics 27:1108-12. 2011..One of the limiting factors is that of chemically identifying metabolites from mass spectrometric signals present in complex datasets...
Array-based evolution of DNA aptamers allows modelling of an explicit sequence-fitness landscapeChristopher G Knight
Manchester Interdisciplinary Biocentre, The University of Manchester, Manchester, UK
Nucleic Acids Res 37:e6. 2009..We demonstrate an extension to the approach by incorporating prior knowledge into CLADE, resulting in some of the tightest binding sequences...
Analysis of aptamer sequence activity relationshipsMark Platt
Manchester Interdisciplinary Biocentre, The University of Manchester, UK
Integr Biol (Camb) 1:116-22. 2009....
KiPar, a tool for systematic information retrieval regarding parameters for kinetic modelling of yeast metabolic pathwaysIrena Spasic
Manchester Centre for Integrative Systems Biology, The University of Manchester, Manchester, UK
Bioinformatics 25:1404-11. 2009..It is also suitable for large-scale mining, since multiple reactions and their kinetic parameters can be specified in a single search request, rather than one reaction at a time, which is unsuitable given the size of genome-scale models...
Multiobjective optimization in bioinformatics and computational biologyJulia Handl
School of Chemistry, The University of Manchester, Manchester, UK
IEEE/ACM Trans Comput Biol Bioinform 4:279-92. 2007....
Systematic integration of experimental data and models in systems biologyPeter Li
School of Chemistry, The University of Manchester, Manchester M13 9PL, UK
BMC Bioinformatics 11:582. 2010..Automating the assembly and use of systems biology models is dependent upon data integration processes involving the interoperation of data and analytical resources...
Visualising biological data: a semantic approach to tool and database integrationSteve Pettifer
School of Computer Science, University of Manchester, Manchester, M13 9PL, UK
BMC Bioinformatics 10:S19. 2009....
'Metabolite-likeness' as a criterion in the design and selection of pharmaceutical drug librariesPaul D Dobson
School of Chemistry and The Manchester Interdisciplinary Biocentre, The University of Manchester, 131 Princess St, Manchester M1 7DN, UK
Drug Discov Today 14:31-40. 2009....
Information-theoretic sensitivity analysis: a general method for credit assignment in complex networksNiklas Lüdtke
School of Chemistry, University of Manchester, 131 Princess Street, Manchester M1 7DN, UK
J R Soc Interface 5:223-35. 2008..Unlike variance-based approaches, our novel methodology can easily accommodate correlated inputs...
Development and performance of a gas chromatography-time-of-flight mass spectrometry analysis for large-scale nontargeted metabolomic studies of human serumPaul Begley
Bioanalytical Sciences Group, School of Chemistry, Manchester Interdisciplinary Biocentre, University of Manchester, M1 7DN, UK
Anal Chem 81:7038-46. 2009..This methodology allows the continuous acquisition and application of data acquired over many months in long-term metabolomic studies, including the HUSERMET project (http://www.husermet.org/)...
Development of a robust and repeatable UPLC-MS method for the long-term metabolomic study of human serumEva Zelena
Bioanalytical Sciences Group, School of Chemistry, Manchester Interdisciplinary Biocentre, University of Manchester, M1 7DN, UK
Anal Chem 81:1357-64. 2009..This method allows the acquisition of data and subsequent comparison of data collected across many months or years...
Metabolic profiling of serum using Ultra Performance Liquid Chromatography and the LTQ-Orbitrap mass spectrometry systemWarwick B Dunn
Manchester Centre for Integrative Systems Biology, School of Chemistry, The Manchester Interdisciplinary Biocentre, The University of Manchester, 131 Princess Street, Manchester, M1 7DN, UK
J Chromatogr B Analyt Technol Biomed Life Sci 871:288-98. 2008..The data were used to indicate possible biomarkers of pre-eclampsia and showed both the instruments and XCMS to be applicable to the reproducible and valid detection of disease biomarkers present in serum...
maxdLoad2 and maxdBrowse: standards-compliant tools for microarray experimental annotation, data management and disseminationDavid Hancock
School of Computer Science, The University of Manchester, Kilburn Building, Oxford Road, Manchester, UK
BMC Bioinformatics 6:264. 2005..maxdBrowse is a PHP web-application that makes contents of maxdLoad2 databases accessible via web-browser, the command-line and web-service environments. It thus acts as both a dissemination and data-mining tool...
Carrier-mediated cellular uptake of pharmaceutical drugs: an exception or the rule?Paul D Dobson
School of Chemistry and Manchester Interdisciplinary Biocentre, University of Manchester, 131 Princess Street, Manchester M1 7DN, UK
Nat Rev Drug Discov 7:205-20. 2008....
Efficient discovery of anti-inflammatory small-molecule combinations using evolutionary computingBen G Small
Doctoral Training Centre, Integrative Systems Biology Molecules to Life, Manchester Centre for Integrative Systems Biology, Manchester Interdisciplinary Biocentre, The University of Manchester, Manchester, UK
Nat Chem Biol 7:902-8. 2011..Evolutionary searches provide a powerful and general approach to the discovery of new combinations of pharmacological agents with therapeutic indices potentially greater than those of single drugs...
Predicting the points of interaction of small molecules in the NF-κB pathwayYogendra Patel
Manchester Interdisciplinary Biocentre, University of Manchester, 131 Princess Street, Manchester M1 7DN, UK
BMC Syst Biol 5:32. 2011..Here we show it can be applied to identify the interactions of small molecules within the NF-κB signalling pathway...
Identification and characterization of high-flux-control genes of yeast through competition analyses in continuous culturesDaniela Delneri
Faculty of Life Sciences, The University of Manchester, Michael Smith Building, Oxford Road, Manchester M13 9PT, UK
Nat Genet 40:113-7. 2008..This chromosome determines a yeast's mating type, and the concentration of haploinsufficient genes there may be a mechanism to prevent its loss...
Selective detection of proteins in mixtures using electrospray ionization mass spectrometry: influence of instrumental settings and implications for proteomicsSeetharaman Vaidyanathan
Department of Chemistry, Faraday Building, Sackville Street, UMIST, P O Box 88, Manchester M60 1QD, UK
Anal Chem 76:5024-32. 2004....
Computational cluster validation in post-genomic data analysisJulia Handl
School of Chemistry, University of Manchester, Faraday Building, Sackville Street, PO Box 88, Manchester M60 1QD, UK
Bioinformatics 21:3201-12. 2005..Suitable computational cluster validation techniques are available in the general data-mining literature, but have been given only a fraction of the same attention in bioinformatics...
Implications of the dominant role of transporters in drug uptake by cellsPaul D Dobson
School of Chemistry and Manchester Interdisciplinary Biocentre, The University of Manchester, 131 Princess Street, Manchester M17DN, UK
Curr Top Med Chem 9:163-81. 2009..Finally, the central importance of computational modelling of transporter substrate preferences by structure-activity relationships is discussed...
Proximate parameter tuning for biochemical networks with uncertain kinetic parametersStephen J Wilkinson
School of Chemistry, Princess St, Manchester, UK
Mol Biosyst 4:74-97. 2008....
Metabolomics and systems biology: making sense of the soupDouglas B Kell
Department of Chemistry, UMIST, Faraday Building, Sackville St, PO Box 88, Manchester M60 1QD, UK
Curr Opin Microbiol 7:296-307. 2004....
Event extraction for systems biology by text mining the literatureSophia Ananiadou
University of Manchester, Manchester M13 9PL, UK
Trends Biotechnol 28:381-90. 2010..The approaches described will be of considerable value in associating particular pathways and their components with higher-order physiological properties, including disease states...
Arcadia: a visualization tool for metabolic pathwaysAlice C Villéger
School of Chemistry and Manchester Interdisciplinary Biocentre, University of Manchester, 131 Princess Street, Manchester M1 7DN, UK
Bioinformatics 26:1470-1. 2010..AVAILABILITY AND IMPLEMENTATION: Arcadia is written in C++. The source code is available (along with Mac OS and Windows binaries) under the GPL from http://arcadiapathways.sourceforge.net/...
Aptamer evolution for array-based diagnosticsMark Platt
Manchester Interdisciplinary Biocenter, University of Manchester, Manchester M1 7DN, UK
Anal Biochem 390:203-5. 2009..The best aptameric sequence was void of the set of four guanine repeats typifying thrombin aptamers and, thus, highlights the benefits of evolution performed in an environment closely mimicking the final diagnostic application...
Convergent evolution to an aptamer observed in small populations on DNA microarraysW Rowe
Manchester Interdisciplinary Biocentre, The University of Manchester, Manchester, UK
Phys Biol 7:036007. 2010..Regime specific differences in the evolutions, such as speed of convergence, could also be observed...
Mass spectrometry tools and metabolite-specific databases for molecular identification in metabolomicsM Brown
Bioanalytical Sciences Group, School of Chemistry, Manchester Interdisciplinary Biocentre, University of Manchester, UK M1 7DN
Analyst 134:1322-32. 2009..To provide definitive identification metabolite-specific mass spectral libraries for UPLC-MS and GC-MS have been constructed for 1,065 commercially available authentic standards. The MMD data are available at http://dbkgroup.org/MMD/...
Utopia documents: linking scholarly literature with research dataT K Attwood
School of Computer Science, Faculty of Life Sciences, University of Manchester, Manchester, UK
Bioinformatics 26:i568-74. 2010..The need for intelligent tools to bridge this gap, to rescue the knowledge being systematically isolated in literature and data silos, is now widely acknowledged...
A systematic survey of the response of a model NF-κB signalling pathway to TNFα stimulationYunjiao Wang
Manchester Interdisciplinary Biocentre, University of Manchester, Manchester M1 7DN, UK
J Theor Biol 297:137-47. 2012....
Growth control of the eukaryote cell: a systems biology study in yeastJuan I Castrillo
Faculty of Life Sciences, Michael Smith Building, University of Manchester, Oxford Road, Manchester M13 9PT, UK
J Biol 6:4. 2007..Comprehensive studies at the transcriptional, proteomic and metabolic levels under defined controlled conditions are currently lacking...
Text mining and its potential applications in systems biologySophia Ananiadou
School of Computer Science, National Centre for Text Mining, The Manchester Interdisciplinary Biocentre, The University of Manchester, 131 Princess Street, Manchester M1 7ND, UK
Trends Biotechnol 24:571-9. 2006..By adding meaning to text, these techniques produce a more structured analysis of textual knowledge than simple word searches, and can provide powerful tools for the production and analysis of systems biology models...
Closed-loop, multiobjective optimization of two-dimensional gas chromatography/mass spectrometry for serum metabolomicsWarwick B Dunn
School of Chemistry, The University of Manchester, Faraday Building, Sackville Street, Manchester M60 1QD, UK
Anal Chem 79:464-76. 2007..A variety of computational methods served to explain the basis for the improvement. This closed-loop optimization strategy is a generic and powerful approach for the optimization of any analytical instrumentation...
Something from nothing: bridging the gap between constraint-based and kinetic modellingKieran Smallbone
Manchester Centre for Integrative Systems Biology, The University of Manchester, UK
FEBS J 274:5576-85. 2007..Moreover, using this particular methodology affords us analytical forms for steady state determination, stability analyses and studies of dynamical behaviour...
Deterministic mathematical models of the cAMP pathway in Saccharomyces cerevisiaeThomas Williamson
Faculty of Life Sciences, The University of Manchester, Manchester, M13 9PT, UK
BMC Syst Biol 3:70. 2009..A stochastic model of the pathway has been reported...
Closed-loop, multiobjective optimization of analytical instrumentation: gas chromatography/time-of-flight mass spectrometry of the metabolomes of human serum and of yeast fermentationsWarwick B Dunn
School of Chemistry, University of Manchester, Faraday Building, Sackville Street, P.O. Box 88, Manchester M60 1QD, U.K
Anal Chem 77:290-303. 2005..The evolutionary closed-loop machine learning strategy we describe is generic to any type of analytical optimization...
Insights into the behaviour of systems biology models from dynamic sensitivity and identifiability analysis: a case study of an NF-kappaB signalling pathwayHong Yue
School of Chemistry, University of Manchester, Sackville St, Manchester, UK
Mol Biosyst 2:640-9. 2006..The whole analysis scheme we describe provides efficient parameter estimation techniques for complex cell networks...
Defrosting the digital library: bibliographic tools for the next generation webDuncan Hull
School of Chemistry, The University of Manchester, Manchester, UK
PLoS Comput Biol 4:e1000204. 2008....
Automated tracking of gene expression in individual cells and cell compartmentsHailin Shen
School of Chemistry, The University of Manchester, Faraday Building, Sackville Street, PO Box 88, Manchester M60 1QD, UK
J R Soc Interface 3:787-94. 2006..This will lead to substantial benefits for time-lapse-based high-content screening...
Genotype-phenotype mapping: genes as computer programsDouglas B Kell
Dept of Chemistry, UMIST, Manchester, UK
Trends Genet 18:555-9. 2002..In this way, the encoding of cellular and higher-order activities by genes is seen as directly analogous to computer programs. This analogy is of utility in biological genetics and in problems of genotype-phenotype mapping...
Synergistic control of oscillations in the NF-kappaB signalling pathwayA E C Ihekwaba
School of Chemistry, The University of Manchester, UK
Syst Biol (Stevenage) 152:153-60. 2005....
Here is the evidence, now what is the hypothesis? The complementary roles of inductive and hypothesis-driven science in the post-genomic eraDouglas B Kell
Department of Chemistry, UMIST, Manchester, UK
Bioessays 26:99-105. 2004..Many fields are data-rich but hypothesis-poor. Here, computational methods of data analysis, which may be automated, provide the means of generating novel hypotheses, especially in the post-genomic era...
Explanatory optimization of protein mass spectrometry via genetic searchSeetharaman Vaidyanathan
Department of Chemistry, UMIST, P.O. Box 88, Sackville Street, Manchester M60 1QD, UK
Anal Chem 75:6679-86. 2003....
Systematic functional analysis of the yeast genomeS G Oliver
Department of Biomolecular Sciences, UMIST, Manchester, UK
Trends Biotechnol 16:373-8. 1998..Systematic and comprehensive approaches to the elucidation of yeast gene function are discussed and the prospects for the functional genomics of eukaryotic organisms evaluated...
Metabolomics and machine learning: explanatory analysis of complex metabolome data using genetic programming to produce simple, robust rulesDouglas B Kell
Institute of Biological Sciences, University of Wales, Aberystwyth, UK
Mol Biol Rep 29:237-41. 2002
Schemes of flux control in a model of Saccharomyces cerevisiae glycolysisLeighton Pritchard
Institute of Biological Sciences, University of Wales, Aberystwyth, UK
Eur J Biochem 269:3894-904. 2002....
Monitoring of complex industrial bioprocesses for metabolite concentrations using modern spectroscopies and machine learning: application to gibberellic acid productionAoife C McGovern
Institute of Biological Sciences, Cledwyn Building, University of Wales, Aberystwyth, Ceredigion SY23 3DD, Wales, UK
Biotechnol Bioeng 78:527-38. 2002....
High-throughput classification of yeast mutants for functional genomics using metabolic footprintingJess Allen
Institute of Biological Sciences, Cledwyn Building, University of Wales, Aberystwyth, Aberystwyth SY23 3DD, UK
Nat Biotechnol 21:692-6. 2003..By using appropriate clustering and machine learning techniques, the latter based on genetic programming, we show that metabolic footprinting is an effective method to classify 'unknown' mutants by genetic defect...
Functional genomic hypothesis generation and experimentation by a robot scientistRoss D King
Department of Computer Science, University of Wales, Aberystwyth SY23 3DB, UK
Nature 427:247-52. 2004....
A proposed framework for the description of plant metabolomics experiments and their resultsHelen Jenkins
Department of Computer Science, University of Wales, Penglais, Aberystwyth, Ceredigion, Wales, UK
Nat Biotechnol 22:1601-6. 2004..armet.org/). We seek to advance discussion and community adoption of a standard for metabolomics, which would promote principled collection, storage and transmission of experiment data...
Metabolomic biomarkers: search, discovery and validationDouglas B Kell
Expert Rev Mol Diagn 7:329-33. 2007
Fast automatic registration of images using the phase of a complex wavelet transform: application to proteome gelsAndrew M Woodward
Institute of Biological Sciences, Cledwyn Building, University of Wales, Aberystwyth, Ceredigion, UKSY23 3DD
Analyst 129:542-52. 2004..The method compares favourably with others, since it is computationally rapid, effective and entirely automatic...
Discrimination of modes of action of antifungal substances by use of metabolic footprintingJess Allen
Department of Biological Sciences, University of Wales, Aberystwyth, UK
Appl Environ Microbiol 70:6157-65. 2004..Metabolic footprinting thus represents a rapid, convenient, and information-rich method for classifying the modes of action of antifungal substances...
Rapid and quantitative detection of the microbial spoilage of meat by fourier transform infrared spectroscopy and machine learningDavid I Ellis
Institute of Biological Sciences. Department of Computer Sciences, University of Wales, Aberystwyth, Ceredigion SY23 3DD, Wales, United Kingdom
Appl Environ Microbiol 68:2822-8. 2002..We believe this approach will aid in the Hazard Analysis Critical Control Point process for the assessment of the microbiological safety of food at the production, processing, manufacturing, packaging, and storage levels...
Flow-injection electrospray ionization mass spectrometry of crude cell extracts for high-throughput bacterial identificationSeetharaman Vaidyanathan
Institute of Biological Sciences, University of Wales, Aberystwyth, Ceredigion, United Kingdom
J Am Soc Mass Spectrom 13:118-28. 2002....
Adoption of the transiently non-culturable state--a bacterial survival strategy?Galina V Mukamolova
Institute of Biological Sciences, University of Wales, Aberystwyth, Ceredigion SY23 3DD, UK
Adv Microb Physiol 47:65-129. 2003....
