Stephen E Kearsey

Summary

Affiliation: University of Oxford
Country: UK

Publications

  1. pmc Rapid regulation of protein activity in fission yeast
    Cathrine A Bøe
    Department of Cell Biology, Rikshospitalet Radiumhospitalet Medical Centre, Montebello, 0310 Oslo, Norway
    BMC Cell Biol 9:23. 2008
  2. ncbi request reprint The role of MCM proteins in the cell cycle control of genome duplication
    S E Kearsey
    Department of Zoology, University of Oxford, England
    Bioessays 18:183-90. 1996
  3. ncbi request reprint Enigmatic variations: divergent modes of regulating eukaryotic DNA replication
    Stephen E Kearsey
    Department of Zoology, South Parks Road, Oxford OX1 3PS, United Kingdom
    Mol Cell 12:1067-75. 2003
  4. pmc Surveying genome replication
    Stephen Kearsey
    Department of Zoology, University of Oxford, South Parks Road, Oxford OX1 3PS, UK
    Genome Biol 3:REVIEWS1016. 2002
  5. pmc Chromatin binding of the fission yeast replication factor mcm4 occurs during anaphase and requires ORC and cdc18
    S E Kearsey
    Department of Zoology, South Parks Road, Oxford OX1 3PS, UK
    EMBO J 19:1681-90. 2000
  6. pmc GINS inactivation phenotypes reveal two pathways for chromatin association of replicative alpha and epsilon DNA polymerases in fission yeast
    Chen Chun Pai
    Department of Zoology, University of Oxford, Oxford OX1 3PS, United Kingdom
    Mol Biol Cell 20:1213-22. 2009
  7. doi request reprint Ribonucleotide reductase activity is coupled to DNA synthesis via proliferating cell nuclear antigen
    Israel Salguero
    Department of Zoology, University of Oxford, Oxford OX1 3PS, UK
    Curr Biol 22:720-6. 2012
  8. pmc Fission yeast Cdc23/Mcm10 functions after pre-replicative complex formation to promote Cdc45 chromatin binding
    Juraj Gregan
    Department of Zoology, University of Oxford, Oxford, OX1 3PS, United Kingdom
    Mol Biol Cell 14:3876-87. 2003
  9. doi request reprint Conditional inactivation of replication proteins in fission yeast using hormone-binding domains
    Chen Chun Pai
    Department of Zoology, University of Oxford, Oxford, UK
    Methods 57:227-33. 2012
  10. ncbi request reprint Analysis of Mcm2-7 chromatin binding during anaphase and in the transition to quiescence in fission yeast
    Mandana Namdar
    Department of Zoology, University of Oxford, South Parks Road, Oxford, OX1 3PS, UK
    Exp Cell Res 312:3360-9. 2006

Collaborators

Detail Information

Publications26

  1. pmc Rapid regulation of protein activity in fission yeast
    Cathrine A Bøe
    Department of Cell Biology, Rikshospitalet Radiumhospitalet Medical Centre, Montebello, 0310 Oslo, Norway
    BMC Cell Biol 9:23. 2008
    ....
  2. ncbi request reprint The role of MCM proteins in the cell cycle control of genome duplication
    S E Kearsey
    Department of Zoology, University of Oxford, England
    Bioessays 18:183-90. 1996
    ..This review will discuss in particular the role of proteins in the minichromosome maintenance (MCM) family, which may hold the key to understanding how DNA is replicated once, and only once, per cell cycle...
  3. ncbi request reprint Enigmatic variations: divergent modes of regulating eukaryotic DNA replication
    Stephen E Kearsey
    Department of Zoology, South Parks Road, Oxford OX1 3PS, United Kingdom
    Mol Cell 12:1067-75. 2003
    ..In spite of this common origin, recent findings have revealed surprising variations in how replication initiation is controlled, implying that a conserved mechanism has not necessarily resulted in regulatory conservation...
  4. pmc Surveying genome replication
    Stephen Kearsey
    Department of Zoology, University of Oxford, South Parks Road, Oxford OX1 3PS, UK
    Genome Biol 3:REVIEWS1016. 2002
    ..Two recent studies have added microarrays to the toolkit used to analyze the origins of replication in yeast chromosomes, providing a fuller picture of how genomic DNA replication is organized...
  5. pmc Chromatin binding of the fission yeast replication factor mcm4 occurs during anaphase and requires ORC and cdc18
    S E Kearsey
    Department of Zoology, South Parks Road, Oxford OX1 3PS, UK
    EMBO J 19:1681-90. 2000
    ..Upon overexpressing cdc18, we show that mcm4 is required for re-replication of the genome in the absence of mitosis and is associated with chromatin in cells undergoing re-replication...
  6. pmc GINS inactivation phenotypes reveal two pathways for chromatin association of replicative alpha and epsilon DNA polymerases in fission yeast
    Chen Chun Pai
    Department of Zoology, University of Oxford, Oxford OX1 3PS, United Kingdom
    Mol Biol Cell 20:1213-22. 2009
    ..We suggest that GINS functions in a pathway that involves Cdc45 and is necessary for DNA polymerase epsilon chromatin binding, but that a separate pathway sets up the chromatin association of DNA polymerase alpha...
  7. doi request reprint Ribonucleotide reductase activity is coupled to DNA synthesis via proliferating cell nuclear antigen
    Israel Salguero
    Department of Zoology, University of Oxford, Oxford OX1 3PS, UK
    Curr Biol 22:720-6. 2012
    ..This mechanism thus provides a direct link between DNA synthesis and RNR regulation...
  8. pmc Fission yeast Cdc23/Mcm10 functions after pre-replicative complex formation to promote Cdc45 chromatin binding
    Juraj Gregan
    Department of Zoology, University of Oxford, Oxford, OX1 3PS, United Kingdom
    Mol Biol Cell 14:3876-87. 2003
    ..On return to growth, Cdc23 chromatin binding is rapidly reestablished independently from pre-RC formation, suggesting that chromatin association of Cdc23 provides a link between proliferation and competence to execute DNA replication...
  9. doi request reprint Conditional inactivation of replication proteins in fission yeast using hormone-binding domains
    Chen Chun Pai
    Department of Zoology, University of Oxford, Oxford, UK
    Methods 57:227-33. 2012
    ..In this article, we describe the application of this method in fission yeast, specifically with regard to analysis of the function of GINS, an essential component of the eukaryotic replicative helicase, the CMG complex...
  10. ncbi request reprint Analysis of Mcm2-7 chromatin binding during anaphase and in the transition to quiescence in fission yeast
    Mandana Namdar
    Department of Zoology, University of Oxford, South Parks Road, Oxford, OX1 3PS, UK
    Exp Cell Res 312:3360-9. 2006
    ..We also examined Mcm2-7 chromatin association when cells enter a G0-like quiescent state. Chromatin binding is lost in this transition in a process that does not require DNA replication or the selective degradation of specific subunits...
  11. pmc Nuclear distribution and chromatin association of DNA polymerase alpha-primase is affected by TEV protease cleavage of Cdc23 (Mcm10) in fission yeast
    Xiaowen Yang
    Department of Zoology, University of Oxford, South Parks Road, Oxford OX13PS, UK
    BMC Mol Biol 6:13. 2005
    ..Cdc23/Mcm10 is required for the initiation and elongation steps of DNA replication but its biochemical function is unclear. Here, we probe its function using a novel approach in fission yeast, involving Cdc23 cleavage by the TEV protease...
  12. pmc Break-induced ATR and Ddb1-Cul4(Cdt)² ubiquitin ligase-dependent nucleotide synthesis promotes homologous recombination repair in fission yeast
    Jennifer Moss
    Department of Oncology, Cancer Research UK Medical Research Council Gray Institute for Radiation Oncology and Biology, University of Oxford, United Kingdom
    Genes Dev 24:2705-16. 2010
    ..Our findings support a model in which break-induced Rad3 and Ddb1-Cul4(Cdt)² ubiquitin ligase-dependent Spd1 degradation and RNR activation promotes postsynaptic ssDNA gap filling during HR repair...
  13. pmc Sequential steps in DNA replication are inhibited to ensure reduction of ploidy in meiosis
    Hui Hua
    Department of Zoology, University of Oxford, Oxford OX1 3PS, United Kingdom
    Mol Biol Cell 24:578-87. 2013
    ..In addition, DNA replication or damage during the meiosis I-II interval fails to arrest meiotic progress, suggesting absence of checkpoint regulation of meiosis II entry...
  14. ncbi request reprint In situ assay for analyzing the chromatin binding of proteins in fission yeast
    Stephen E Kearsey
    Department of Zoology, University of Oxford, Oxford, UK
    Methods Mol Biol 296:181-8. 2005
    ..This method allows the chromatin association of proteins to be correlated with other cell cycle events without the need for cell synchronization...
  15. pmc Cdt1 proteolysis is promoted by dual PIP degrons and is modulated by PCNA ubiquitylation
    Estrella Guarino
    Department of Zoology, University of Oxford, South Parks Road, Oxford OX1 3PS, UK
    Nucleic Acids Res 39:5978-90. 2011
    ..This provides a mechanism for fine-tuning the activity of the CRL4(Cdt2) pathway towards Cdt1, allowing Cdt1 proteolysis to be more efficient in S phase than after DNA damage...
  16. doi request reprint Using the DHFR heat-inducible degron for protein inactivation in Schizosaccharomyces pombe
    Stephen E Kearsey
    Department of Zoology, University of Oxford, Oxford, UK
    Methods Mol Biol 521:483-92. 2009
    ..We describe here the application of this technique in the fission yeast, Schizosaccharomyces pombe...
  17. ncbi request reprint A yeast model for the study of human DFNA5, a gene mutated in nonsyndromic hearing impairment
    Juraj Gregan
    Department of Zoology, University of Oxford, South Parks Road, Oxford OX1 3PS, UK
    Biochim Biophys Acta 1638:179-86. 2003
    ..Genetic interactions between heterologously expressed DFNA5 and a fission yeast cdc23 (mcm10) mutant support a possible link between DFNA5 and Mcm10 proteins...
  18. pmc Essential role of MCM proteins in premeiotic DNA replication
    Karola Lindner
    Department of Zoology, University of Oxford, Oxford, OX1 3PS United Kingdom
    Mol Biol Cell 13:435-44. 2002
    ..Together, these results emphasize similarity between replication mechanisms in mitotic and meiotic cell cycles...
  19. pmc Fission yeast Cut8 is required for the repair of DNA double-strand breaks, ribosomal DNA maintenance, and cell survival in the absence of Rqh1 helicase
    Stephen E Kearsey
    Department of Zoology, South Parks Road, Oxford OX1 3PS, United Kingdom
    Mol Cell Biol 27:1558-67. 2007
    ..pombe...
  20. pmc DNA damage induces Cdt1 proteolysis in fission yeast through a pathway dependent on Cdt2 and Ddb1
    Emma Ralph
    Department of Zoology, University of Oxford, South Parks Road, Oxford OX1 3PS, UK
    EMBO Rep 7:1134-9. 2006
    ....
  21. pmc Germline mutations affecting the proofreading domains of POLE and POLD1 predispose to colorectal adenomas and carcinomas
    Claire Palles
    Molecular and Population Genetics Laboratory, Wellcome Trust Centre for Human Genetics, University of Oxford, Oxford, UK
    Nat Genet 45:136-44. 2013
    ..Further analysis of published data showed that the recently described group of hypermutant, microsatellite-stable CRCs is likely to be caused by somatic POLE mutations affecting the exonuclease domain...
  22. pmc Monitoring DNA replication in fission yeast by incorporation of 5-ethynyl-2'-deoxyuridine
    Hui Hua
    Department of Zoology, University of Oxford, South Parks Road, Oxford, OX1 3PS, UK
    Nucleic Acids Res 39:e60. 2011
    ..Thus EdU should be useful for detecting early stages of S phase, or DNA synthesis associated with DNA repair and recombination...
  23. pmc The C-terminal zinc finger of the catalytic subunit of DNA polymerase delta is responsible for direct interaction with the B-subunit
    Javier Sanchez Garcia
    Wellcome Trust Centre for Cell Biology, University of Edinburgh, Michael Swann Building, King s Buildings, Mayfield Road, Edinburgh EH9 3JR, UK
    Nucleic Acids Res 32:3005-16. 2004
    ..Finally, evidence is presented suggesting that the ZnF region is post-translationally modified in fission yeast cells...
  24. pmc Global role for polyadenylation-assisted nuclear RNA degradation in posttranscriptional gene silencing
    Shao Win Wang
    Division of Molecular and Genomic Medicine, National Health Research Institutes, 35 Keyan Road, Zhunan Town, Miaoli County 350, Taiwan
    Mol Cell Biol 28:656-65. 2008
    ....
  25. pmc The kinetochore proteins Pcs1 and Mde4 and heterochromatin are required to prevent merotelic orientation
    Juraj Gregan
    Max F Perutz Laboratories, Department of Chromosome Biology, University of Vienna, Dr Bohr Gasse 1, 1030 Vienna, Austria
    Curr Biol 17:1190-200. 2007
    ..A protein called Pcs1 has been implicated in preventing merotelic attachment in mitosis and meiosis II in the fission yeast S. pombe...
  26. pmc A novel checkpoint mechanism regulating the G1/S transition
    Tonje Tvegård
    Department of Cell Biology, Rikshospitalet Radiumhospitalet Medical Centre and University of Oslo, Montebello, 0310 Oslo, Norway
    Genes Dev 21:649-54. 2007
    ..The cell cycle delay represents a novel checkpoint with a novel mechanism of action that is not activated by ionizing radiation...