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Species | K T JonesSummaryAffiliation: University of Newcastle Country: UK Publications
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Publications
Intracellular calcium in the fertilization and development of mammalian eggsKeith T Jones
Institute for Cell and Molecular Biosciences, The Medical School, Framlington Place, University of Newcastle, Newcastle, UK
Clin Exp Pharmacol Physiol 34:1084-9. 2007..The nature of the signals remains little explored, but their importance is clear and so warrants further investigation...- Turning it on and off: M-phase promoting factor during meiotic maturation and fertilizationKeith T Jones
Cell and Developmental Physiology Research Group, School of Cell and Molecular Biosciences, The Medical School, Framlington Place, University of Newcastle, Newcastle NE2 4HH, UK
Mol Hum Reprod 10:1-5. 2004..An understanding of how oocytes switch it on and turn it off underpins much of the basic cell biology of oocyte maturation... 
Different Ca2+-releasing abilities of sperm extracts compared with tissue extracts and phospholipase C isoforms in sea urchin egg homogenate and mouse eggsK T Jones
Department of Anatomy, University College, Gower Street, London, WC1E 6BT, UK
Biochem J 346:743-9. 2000..In addition these PLCs were not able to cause Ca(2+) oscillations following microinjection into mouse eggs. These results imply that the sperm PLC possesses distinct properties that allow it to hydrolyse PtdIns(4,5)P(2) in eggs...- Sperm-induced Ca(2+) oscillations in mouse oocytes and eggs can be mimicked by photolysis of caged inositol 1,4,5-trisphosphate: evidence to support a continuous low level production of inositol 1, 4,5-trisphosphate during mammalian fertilizationK T Jones
Department of Physiological Sciences, The Medical School, University of Newcastle, Framlington Place, Newcastle, NE2 4HH, United Kingdom
Dev Biol 225:1-12. 2000..It is proposed that a sperm Ca(2+)-releasing factor operates by generating a continuous small amount of InsP(3) over an extended period of time, consistent with the evidence for the involvement of a phospholipase C... - Meiosis in oocytes: predisposition to aneuploidy and its increased incidence with ageKeith T Jones
Institute for Cell and Molecular Biosciences, The Medical School, University of Newcastle, Framlington Place, Newcastle, NE2 4HH, UK
Hum Reprod Update 14:143-58. 2008.... - Composition of sea urchin egg homogenate determines its potency to inositol trisphosphate and cyclic ADPRibose-induced Ca2+ releaseKeith T Jones
Institute for Cell and Molecular Biosciences, The Medical School, Framlington Place, University of Newcastle, Newcastle NE2 4HH, UK
Biochem Biophys Res Commun 360:815-20. 2007..Conversely the response to cADPR was diminished, whilst NAADP was unaffected. Therefore modifying media composition may be an important consideration in using homogenate to study Ca2+ release for future studies... - Ca2+ oscillations and the cell cycle at fertilisation of mammalian and ascidian eggsV L Nixon
Department of Physiological Sciences, The Medical School, University of Newcastle upon Tyne, UK
Biol Cell 92:187-96. 2000..Here, we explore the nature of the sperm Ca2+-releasing factor and examine the relationship between cell cycle resumption and the control of Ca2+ oscillations at fertilisation... - Simultaneous measurement of intracellular nitric oxide and free calcium levels in chordate eggs demonstrates that nitric oxide has no role at fertilizationL A Hyslop
Department of Physiological Sciences, The Medical School, Framlington Place, The University of Newcastle, Newcastle, NE2 4HH, England, UK
Dev Biol 234:216-30. 2001..Therefore, we suggest that the current data provide evidence that NO has no role in the fertilization of these two chordate eggs... - A mammalian sperm cytosolic phospholipase C activity generates inositol trisphosphate and causes Ca2+ release in sea urchin egg homogenatesK T Jones
Department of Anatomy and Developmental Biology, University College, London, UK
FEBS Lett 437:297-300. 1998..These data indicate that sperm extracts contain an InsP3-generating phospholipase C which may play a role in Ca2+ release at fertilisation... - Ca(2+) oscillations promote APC/C-dependent cyclin B1 degradation during metaphase arrest and completion of meiosis in fertilizing mouse eggsVictoria L Nixon
Department of Physiological Sciences, The Medical School, Framlington Place, University of Newcastle, Newcastle NE2 4HH, United Kingdom
Curr Biol 12:746-50. 2002..Therefore, the physiological need for a repetitive Ca(2+) signal in mammals is to ensure long-term cyclin destruction during a protracted exit from meiosis... - Potential role of a sperm-derived phospholipase C in triggering the egg-activating Ca2+ signal at fertilizationK Swann
Department of Anatomy and Developmental Biology, Gower Street, University College, London WC1E 6BT, UK
Reproduction 122:839-46. 2001..This evidence leads us to propose that, after gamete fusion, a sperm-derived phospholipase C causes production of inositol 1,4,5- trisphosphate, which then generates Ca2+ waves from within the egg cytoplasm... - Exploring the mechanism of action of the sperm-triggered calcium-wave pacemaker in ascidian zygotesMichael Carroll
Cell and Developmental Physiology Group, School of Cell and Molecular Biosciences, The Medical School, University of Newcastle upon Tyne, Framlington Place, Newcastle upon Tyne NE2 4HH, UK
J Cell Sci 116:4997-5004. 2003..We suggest that the Ca2+-releasing sperm factor might be tethered near or on the PM and that following the cortical contraction, it is translocated to the vegetal CP, thus making that site act as a Ca2+-wave pacemaker... - Degradation of APCcdc20 and APCcdh1 substrates during the second meiotic division in mouse eggsHeng Yu Chang
Cell and Developmental Physiology Research Group, Institute for Cell and Molecular Biosciences, The Medical School, Framlington Place, University of Newcastle, Newcastle, NE2 4HH, UK
J Cell Sci 117:6289-96. 2004..Interestingly, therefore, we propose that mammalian eggs undergo meiosis II with both APCcdc20 and APCcdh1, whereas eggs of other species so far described have APCcdc20 activity only... - Mammalian egg activation: from Ca2+ spiking to cell cycle progressionKeith T Jones
Institute for Cell and Molecular Biosciences, The Medical School, Framlington Place, University of Newcastle, Newcastle, NE2 4HH, UK
Reproduction 130:813-23. 2005..APC/C activation leads to securin and cyclin B1 degradation and in so doing allows sister chromatids to be segregated and to decondense... - A comparison of sperm- and IP3-induced Ca2+ release in activated and aging mouse oocytesK T Jones
Medical Research Council Experimental Embryology and Teratology Unit, St George s Hospital Medical School, London, United Kingdom
Dev Biol 178:229-37. 1996..The results suggest that factors within the oocyte, such as store size, are important in enabling sperm to generate repetitive Ca2+ transients. Also, the Ca2+ release processes decline as the oocyte ages as well as after activation... - Calmodulin-dependent protein kinase II, and not protein kinase C, is sufficient for triggering cell-cycle resumption in mammalian eggsSuzanne Madgwick
Institute for Cell and Molecular Biosciences, The Medical School, Framlington Place, University of Newcastle, Newcastle, NE2 4HH, UK
J Cell Sci 118:3849-59. 2005..These data, combined with the knowledge that CamKII activity increase at fertilization, suggest that mouse eggs undergo cell-cycle resumption through stimulation of CamKII... - Maintenance of sister chromatid attachment in mouse eggs through maturation-promoting factor activitySuzanne Madgwick
Cell and Developmental Physiology Research Group, Institute of Cell and Molecular Biosciences, The Medical School, Framlington Place, University of Newcastle, NE2 4HH, UK
Dev Biol 275:68-81. 2004..We present a model in which metaphase II arrest is maintained primarily by MPF levels only... - Ca(2+)-promoted cyclin B1 degradation in mouse oocytes requires the establishment of a metaphase arrestLouise A Hyslop
Cell and Developmental Physiology Research Group, School of Cell and Molecular Biosciences, The Medical School, University of Newcastle, Newcastle NE2 4HH, UK
Dev Biol 269:206-19. 2004..However, this stimulation occurs only in the presence of the ubiquitin ligase inhibitor CSF. We propose a model in which Ca2+ directly stimulates destruction of CSF during mammalian fertilisation... - A novel mechanism controls the Ca2+ oscillations triggered by activation of ascidian eggs and has an absolute requirement for Cdk1 activityMark Levasseur
Institute for Cell and Molecular Biosciences, The Medical School, University of Newcastle, Newcastle upon Tyne, UK
J Cell Sci 120:1763-71. 2007..These findings suggest a new link between this cell cycle kinase and the Ins(1,4,5)P(3) pathway... - Mouse Emi2 is required to enter meiosis II by reestablishing cyclin B1 during interkinesisSuzanne Madgwick
Institute for Cell and Molecular Biosciences, The Medical School, University of Newcastle, Newcastle NE2 4HH, England, UK
J Cell Biol 174:791-801. 2006.... - Cell cycle-dependent repetitive Ca(2+ )waves induced by a cytosolic sperm extract in mature ascidian eggs mimic those observed at fertilizationA McDougall
Department of Physiological Sciences, The Medical School, University of Newcastle upon Tyne, Framlington Place, Newcastle upon Tyne NE2 4HH, UK
J Cell Sci 113:3453-62. 2000..We demonstrated that ascidian sperm contain a protein factor(s) that is regulated by the egg CDK activity and can trigger all the Ca(2+ )waves observed at fertilization with a spatial pattern that mimics those initiated by sperm... - Prometaphase APCcdh1 activity prevents non-disjunction in mammalian oocytesAlexandra Reis
Institute for Cell and Molecular Biosciences, The Medical School, Framlington Place, University of Newcastle, Newcastle NE2 4HH, UK
Nat Cell Biol 9:1192-8. 2007..These findings demonstrate a fundamentally different mechanism of control for the first meiotic division in mammalian oocytes that is not observed in meioses of other species... 
Securin and not CDK1/cyclin B1 regulates sister chromatid disjunction during meiosis II in mouse eggsIbtissem Nabti
Institute for Cell and Molecular Biosciences, The Medical School, Framlington Place, University of Newcastle, Newcastle, NE2 4HH, UK
Dev Biol 321:379-86. 2008..In contrast, securin morpholino knockdown specifically induced loss of sister attachment, that could be restored by securin cRNA rescue. During metII arrest separase appears primarily regulated by securin binding, not CDK1/cyclin B1...- The CRY box: a second APCcdh1-dependent degron in mammalian cdc20Alexandra Reis
Institute for Cell and Molecular Biosciences, The Medical School, University of Newcastle upon Tyne, Newcastle upon Tyne NE2 4HH, UK
EMBO Rep 7:1040-5. 2006..Thus, mammalian oocytes and embryos have the capacity to recognize two degrons in cdc20... - APCcdh1 activity in mouse oocytes prevents entry into the first meiotic divisionAlexandra Reis
Institute for Cell and Molecular Biosciences, The Medical School, Framlington Place, University of Newcastle-upon-Tyne, Newcastle, NE2 4HH, UK
Nat Cell Biol 8:539-40. 2006..Here, we find that APCcdh1 is active in mouse oocytes and is necessary to maintain prophase arrest... - Essential CDK1-inhibitory role for separase during meiosis I in vertebrate oocytesIngo H Gorr
Department of Molecular Cell Biology, Max Planck Institute of Biochemistry, Am Klopferspitz 18, 82152 Martinsried, Germany
Nat Cell Biol 8:1035-7. 2006..Importantly, proper meiotic maturation is rescued by chemical inhibition of Cdk1 or expression of Cdk1-binding separase fragments lacking cohesin-cleaving activity... - Calmodulin-dependent protein kinase II triggers mouse egg activation and embryo development in the absence of Ca2+ oscillationsJason G Knott
Center for Research on Reproduction and Women s Health, University of Pennsylvania, Philadelphia, PA 19104, USA
Dev Biol 296:388-95. 2006..These results strongly suggest that CaMKII is a major integrator of the Ca(2+) changes that occur following fertilization... - CaMKII can participate in but is not sufficient for the establishment of the membrane block to polyspermy in mouse eggsAllison J Gardner
Department of Biochemistry and Molecular Biology, Division of Reproductive Biology, Johns Hopkins University, Bloomberg School of Public Health, Baltimore, Maryland 21205, USA
J Cell Physiol 212:275-80. 2007.... - SIAH1 targets the alternative splicing factor T-STAR for degradation by the proteasomeJulian P Venables
Institute of Human Genetics, International Centre for Life, Newcastle upon Tyne, UK
Hum Mol Genet 13:1525-34. 2004..Collectively these data suggest that SIAH-mediated down regulation of alternative splicing may be an important developmental difference between otherwise highly conserved T-STAR proteins...