S P Jackson

Summary

Affiliation: University of Cambridge
Country: UK

Publications

  1. ncbi request reprint ATM- and cell cycle-dependent regulation of ATR in response to DNA double-strand breaks
    Ali Jazayeri
    The Wellcome Trust and Cancer Research UK Gurdon Institute, and Department of Zoology, Cambridge University, Tennis Court Road, Cambridge, CB2 1QN, UK
    Nat Cell Biol 8:37-45. 2006
  2. ncbi request reprint MDC1 directly binds phosphorylated histone H2AX to regulate cellular responses to DNA double-strand breaks
    Manuel Stucki
    The Wellcome Trust Cancer Research UK Gurdon Institute and Department of Zoology, Cambridge University, Tennis Court Road, Cambridge CB2 1QN, United Kingdom
    Cell 123:1213-26. 2005
  3. ncbi request reprint Detecting, signalling and repairing DNA double-strand breaks
    S P Jackson
    The Wellcome Trust and Cancer Research Campaign, Institute of Cancer and Developmental Biology, University of Cambridge, Tennis Court Road, Cambridge CB2 1QR, UK
    Biochem Soc Trans 29:655-61. 2001
  4. ncbi request reprint The recognition of DNA damage
    S P Jackson
    Department of Zoology, Cambridge University, UK
    Curr Opin Genet Dev 6:19-25. 1996
  5. ncbi request reprint Sensing and repairing DNA double-strand breaks
    Stephen P Jackson
    Wellcome Trust and Cancer Research UK Institute of Cancer and Developmental Biology, Tennis Court Road, Cambridge CB2 1QR, UK
    Carcinogenesis 23:687-96. 2002
  6. pmc Screening the yeast genome for new DNA-repair genes
    Ali Jazayeri
    Wellcome Cancer Research UK Institute of Cancer and Developmental Biology and Department of Zoology, University of Cambridge, Tennis Court Road, Cambridge CB2 1QR, UK
    Genome Biol 3:REVIEWS1009. 2002
  7. pmc Telomerase subunit overexpression suppresses telomere-specific checkpoint activation in the yeast yku80 mutant
    S H Teo
    Wellcome Trust and Cancer Research Campaign, Institute of Cancer and Developmental Biology, University of Cambridge, Cambridge CB2 1QR, UK
    EMBO Rep 2:197-202. 2001
  8. ncbi request reprint Effects of DNA nonhomologous end-joining factors on telomere length and chromosomal stability in mammalian cells
    F d'Adda di Fagagna
    Wellcome CRC Institute, University of Cambridge, Tennis Court Road, Cambridge, CB2 1QR, United Kingdom
    Curr Biol 11:1192-6. 2001
  9. ncbi request reprint DNA damage triggers disruption of telomeric silencing and Mec1p-dependent relocation of Sir3p
    A D McAinsh
    Wellcome Trust Cancer Research Campaign Institute, Department of Zoology, University of Cambridge, Tennis Court Road, Cambridge, CB2 1QR, UK
    Curr Biol 9:963-6. 1999
  10. pmc The yeast Xrs2 complex functions in S phase checkpoint regulation
    D D'Amours
    Wellcome Trust and Cancer Research Campaign Institute of Cancer and Developmental Biology, and Department of Zoology, University of Cambridge, CB2 1QR Cambridge, UK
    Genes Dev 15:2238-49. 2001

Detail Information

Publications89

  1. ncbi request reprint ATM- and cell cycle-dependent regulation of ATR in response to DNA double-strand breaks
    Ali Jazayeri
    The Wellcome Trust and Cancer Research UK Gurdon Institute, and Department of Zoology, Cambridge University, Tennis Court Road, Cambridge, CB2 1QN, UK
    Nat Cell Biol 8:37-45. 2006
    ..Thus, in response to DSBs, ATR activation is regulated by ATM in a cell-cycle dependent manner...
  2. ncbi request reprint MDC1 directly binds phosphorylated histone H2AX to regulate cellular responses to DNA double-strand breaks
    Manuel Stucki
    The Wellcome Trust Cancer Research UK Gurdon Institute and Department of Zoology, Cambridge University, Tennis Court Road, Cambridge CB2 1QN, United Kingdom
    Cell 123:1213-26. 2005
    ..Thus, binding of MDC1/NFBD1 to gammaH2AX plays a central role in the mammalian response to DNA damage...
  3. ncbi request reprint Detecting, signalling and repairing DNA double-strand breaks
    S P Jackson
    The Wellcome Trust and Cancer Research Campaign, Institute of Cancer and Developmental Biology, University of Cambridge, Tennis Court Road, Cambridge CB2 1QR, UK
    Biochem Soc Trans 29:655-61. 2001
    ..I also explain how defects in the proteins that function in these pathways are associated with a variety of human pathological states...
  4. ncbi request reprint The recognition of DNA damage
    S P Jackson
    Department of Zoology, Cambridge University, UK
    Curr Opin Genet Dev 6:19-25. 1996
    ..This work also suggests how deficiencies in DNA damage detection systems can result in genetic instability and cancer...
  5. ncbi request reprint Sensing and repairing DNA double-strand breaks
    Stephen P Jackson
    Wellcome Trust and Cancer Research UK Institute of Cancer and Developmental Biology, Tennis Court Road, Cambridge CB2 1QR, UK
    Carcinogenesis 23:687-96. 2002
    ..An increased knowledge of DSB repair and of other DNA DSB responses may therefore provide opportunities for developing more effective treatments for cancer...
  6. pmc Screening the yeast genome for new DNA-repair genes
    Ali Jazayeri
    Wellcome Cancer Research UK Institute of Cancer and Developmental Biology and Department of Zoology, University of Cambridge, Tennis Court Road, Cambridge CB2 1QR, UK
    Genome Biol 3:REVIEWS1009. 2002
    ..The availability of the complete genome sequence of Saccharomyces cerevissiae has greatly facilitated the discovery of new genes important for DNA repair...
  7. pmc Telomerase subunit overexpression suppresses telomere-specific checkpoint activation in the yeast yku80 mutant
    S H Teo
    Wellcome Trust and Cancer Research Campaign, Institute of Cancer and Developmental Biology, University of Cambridge, Cambridge CB2 1QR, UK
    EMBO Rep 2:197-202. 2001
    ....
  8. ncbi request reprint Effects of DNA nonhomologous end-joining factors on telomere length and chromosomal stability in mammalian cells
    F d'Adda di Fagagna
    Wellcome CRC Institute, University of Cambridge, Tennis Court Road, Cambridge, CB2 1QR, United Kingdom
    Curr Biol 11:1192-6. 2001
    ..We also observe higher genomic instability in Ku-deficient cells than in XRCC4-null cells. This suggests that chromosomal instability of Ku-deficient cells results from a combination of compromised telomere stability and defective NHEJ...
  9. ncbi request reprint DNA damage triggers disruption of telomeric silencing and Mec1p-dependent relocation of Sir3p
    A D McAinsh
    Wellcome Trust Cancer Research Campaign Institute, Department of Zoology, University of Cambridge, Tennis Court Road, Cambridge, CB2 1QR, UK
    Curr Biol 9:963-6. 1999
    ....
  10. pmc The yeast Xrs2 complex functions in S phase checkpoint regulation
    D D'Amours
    Wellcome Trust and Cancer Research Campaign Institute of Cancer and Developmental Biology, and Department of Zoology, University of Cambridge, CB2 1QR Cambridge, UK
    Genes Dev 15:2238-49. 2001
    ..These results indicate that the Tel1p/ATM signaling pathway is conserved from yeast to humans and suggest that the Xrs2p/Nbs1 complexes act as signal modifiers...
  11. pmc Components of the Ku-dependent non-homologous end-joining pathway are involved in telomeric length maintenance and telomeric silencing
    S J Boulton
    Wellcome CRC Institute, Tennis Court Road, Cambridge CB2 1QR, UK
    EMBO J 17:1819-28. 1998
    ..These data provide important insights into DNA DSB repair and the linkage of this process to telomere length homeostasis and transcriptional silencing...
  12. ncbi request reprint A role for Saccharomyces cerevisiae histone H2A in DNA repair
    J A Downs
    The Wellcome Trust and Cancer Research Campaign, Institute of Cancer and Developmental Biology, and Department of Zoology, University of Cambridge, UK
    Nature 408:1001-4. 2000
    ..In addition, the motif has a role in determining higher order chromatin structure. Thus, phosphorylation of a core histone in response to DNA damage may cause an alteration of chromatin structure that facilitates DNA repair...
  13. pmc Mapping of protein-protein interactions within the DNA-dependent protein kinase complex
    D Gell
    Wellcome CRC Institute, Tennis Court Road, Cambridge CB2 1QR, UK
    Nucleic Acids Res 27:3494-502. 1999
    ..These data suggest that Ku has evolved to become part of the DNA-PK holo-enzyme by acquisition of a protein-protein interaction motif at the C-terminus of Ku80...
  14. ncbi request reprint The complex matter of DNA double-strand break detection
    J M Bradbury
    The Wellcome Trust Cancer Research UK Institute of Cancer and Developmental Biology, Tennis Court Road, Cambridge CB2 1QR, UK
    Biochem Soc Trans 31:40-4. 2003
    ....
  15. pmc LCD1: an essential gene involved in checkpoint control and regulation of the MEC1 signalling pathway in Saccharomyces cerevisiae
    J Rouse
    Wellcome Trust and Cancer Research Campaign, Institute of Cancer and Developmental Biology, University of Cambridge, Tennis Court Road, Cambridge CB2 1QR, UK
    EMBO J 19:5801-12. 2000
    ..These results indicate that Lcd1p is a pivotal checkpoint regulator, involved in both the essential and checkpoint functions of the Mec1p pathway...
  16. ncbi request reprint The ataxia-telangiectasia related protein ATR mediates DNA-dependent phosphorylation of p53
    N D Lakin
    Wellcome Trust Cancer Research Campaign Institute of Cancer and Developmental Biology, Department of Zoology, Cambridge University, UK
    Oncogene 18:3989-95. 1999
    ..These data suggest that ATR may function upstream of p53 in a signal transduction cascade initiated upon DNA damage and provide a biochemical assay system for ATR activity...
  17. pmc Identification of Saccharomyces cerevisiae DNA ligase IV: involvement in DNA double-strand break repair
    S H Teo
    Wellcome CRC Institute and Department of Zoology, University of Cambridge, UK
    EMBO J 16:4788-95. 1997
    ..Furthermore, they provide insights into mechanisms of DNA repair and suggest that the NHEJ pathway is highly conserved throughout the eukaryotic kingdom...
  18. ncbi request reprint Lif1p targets the DNA ligase Lig4p to sites of DNA double-strand breaks
    S H Teo
    The Wellcome Trust and Cancer Research Campaign, Institute of Cancer and Developmental Biology, University of Cambridge, Cambridge, CB2 1QR, UK
    Curr Biol 10:165-8. 2000
    ..Furthermore, this targeting requires another key NHEJ protein, Ku...
  19. ncbi request reprint Ku, a DNA repair protein with multiple cellular functions?
    C Featherstone
    Wellcome Cancer Research Campaign Institute, Cambridge University, UK
    Mutat Res 434:3-15. 1999
    ..Recent findings have implicated yeast Ku in telomeric structure in addition to NHEJ. Some of the phenotypes of the Ku-knockout mice may indicate a similar role for Ku at mammalian telomeres...
  20. ncbi request reprint A role for TAF3B2 in the repression of human RNA polymerase III transcription in nonproliferating cells
    K Eichhorn
    Wellcome Trust and Cancer Research Campaign Institute of Cancer and Developmental Biology and the Department of Zoology, University of Cambridge, Tennis Court Road, Cambridge CB2 1QR, United Kingdom
    J Biol Chem 276:21158-65. 2001
    ..These findings provide a novel mechanism of Pol III regulation and yield insights into how cellular biosynthetic capacity and growth status can be coordinated...
  21. ncbi request reprint DNA repair: how Ku makes ends meet
    A J Doherty
    Cambridge Institute for Medical Research, Department of Haematology, University of Cambridge, Hills Road, Cambridge CB2 2XY, UK
    Curr Biol 11:R920-4. 2001
    ..The recently determined crystal structure of the Ku heterodimer, in both DNA-bound and unbound forms, has shed new light on the mechanism by which this protein fulfills its key role in the repair of DNA double-strand breaks...
  22. ncbi request reprint DNA-PK, ATM and ATR as sensors of DNA damage: variations on a theme?
    D Durocher
    Wellcome Trust and Cancer Research Campaign Institute of Cancer and Developmental Biology, University of Cambridge, Tennis Court Road, CB2 1QR, Cambridge, UK
    Curr Opin Cell Biol 13:225-31. 2001
    ..Recent work has indicated that the human ATM and ATR proteins, and their yeast homologues, are intimately involved in sensing DNA damage, suggesting parallels with the DNA double-strand break repair enzyme DNA-PK...
  23. ncbi request reprint Basal and regulated transcription in Archaea
    S D Bell
    Wellcome Trust and Cancer Research Campaign Institute of Cancer and Developmental Biology, Tennis Court Road, Cambridge, CB2 1QR, UK
    Biochem Soc Trans 29:392-5. 2001
    ..The mode of action of two such regulators has been characterized to determine how these 'bacterial-like' regulators impinge on the 'eucaryal-like' basal machinery...
  24. pmc Purification and DNA binding properties of the ataxia-telangiectasia gene product ATM
    G C Smith
    Wellcome Trust, Institute of Cancer, Department of Zoology, Tennis Court Road, Cambridge CB2 1QR, United Kingdom
    Proc Natl Acad Sci U S A 96:11134-9. 1999
    ....
  25. pmc Human and mouse homologs of Schizosaccharomyces pombe rad1(+) and Saccharomyces cerevisiae RAD17: linkage to checkpoint control and mammalian meiosis
    R Freire
    Wellcome Trust Cancer Research Campaign Institute of Cancer and Developmental Biology, and Department of Zoology, Cambridge University, Cambridge CB2 1QR, UK
    Genes Dev 12:2560-73. 1998
    ..Together, these data imply an important role for hRad1 both in the mitotic DNA damage checkpoint and in meiotic checkpoint mechanisms, and suggest that these events are highly conserved from yeast to humans...
  26. ncbi request reprint Regulation of p53 in response to DNA damage
    N D Lakin
    Wellcome Trust Cancer Research Campaign, Institute of Cancer and Developmental Biology, Cambridge University, Tennis Court Road, Cambridge CB2 1QR, UK
    Oncogene 18:7644-55. 1999
    ..Here, we discuss the nature of these modifications, the enzymes that bring them about, and how changes in p53 modification lead to p53 activation...
  27. ncbi request reprint Identification of a conserved archaeal RNA polymerase subunit contacted by the basal transcription factor TFB
    C P Magill
    Wellcome Trust and Cancer Research Campaign Institute of Cancer and Developmental Biology, Tennis Court Road, Cambridge CB2 1QR, United Kingdom
    J Biol Chem 276:46693-6. 2001
    ..Intriguingly, homologues of RpoK are found in all three nuclear RNA polymerases (Rpb6) and also in the bacterial RNA polymerase (omega-subunit)...
  28. pmc Identification of a Saccharomyces cerevisiae Ku80 homologue: roles in DNA double strand break rejoining and in telomeric maintenance
    S J Boulton
    Wellcome CRC Institute and Department of Zoology, Cambridge University, UK
    Nucleic Acids Res 24:4639-48. 1996
    ..These findings raise the possibility that Ku protects chromosomal termini from nucleolytic attack and functions as part of a telomeric length sensing system...
  29. pmc DNA end-independent activation of DNA-PK mediated via association with the DNA-binding protein C1D
    U Yavuzer
    Wellcome Trust Cancer Research Campaign Institute of Cancer and Developmental Biology and Department of Zoology, Cambridge University, Cambridge CB2 1QR, UK
    Genes Dev 12:2188-99. 1998
    ..Finally, we establish that C1D directs the activation of DNA-PK in a manner that does not require DNA termini. Therefore, these studies provide a function for C1D and suggest novel mechanisms for DNA-PK activation in vivo...
  30. ncbi request reprint Crystal structure of an Xrcc4-DNA ligase IV complex
    B L Sibanda
    Department of Biochemistry, Cambridge University, Tennis Court Road, Cambridge CB2 1GA, UK
    Nat Struct Biol 8:1015-9. 2001
    ..The strong conservation of residues at the interface between the two proteins provides evidence that the observed mode of interaction has been maintained in NHEJ throughout evolution...
  31. ncbi request reprint Mechanism and regulation of transcription in archaea
    S D Bell
    Wellcome Trust and Cancer Research Campaign Institute of Cancer and Developmental Biology and Department of Zoology, University of Cambridge, Tennis Court Road, CB2 1QR, Cambridge, UK
    Curr Opin Microbiol 4:208-13. 2001
    ..The presence of these shared bacterial-archaeal regulators has given insight into the evolution of gene regulatory processes in all three domains of life...
  32. ncbi request reprint Repression of RNA polymerase III transcription by the retinoblastoma protein
    R J White
    Department of Zoology, University of Cambridge, UK
    Nature 382:88-90. 1996
    ..These results implicate repression of pol III transcription as a mechanism for Rb-induced growth arrest, and suggest that restraining protein biosynthesis may be important in the prevention of tumour development...
  33. ncbi request reprint Functions of poly(ADP-ribose) polymerase in controlling telomere length and chromosomal stability
    F d'Adda di Fagagna
    Wellcome CRC Institute, Tennis Court Road, Cambridge, CB2 1QR, UK
    Nat Genet 23:76-80. 1999
    ..This study therefore reveals an unanticipated role for PARP in telomere length regulation and provides insights into its functions in maintaining genomic integrity...
  34. ncbi request reprint Conserved functional domains of the RNA polymerase III general transcription factor BRF
    B Khoo
    Wellcome CRC Institute, Cambridge University, UK
    Genes Dev 8:2879-90. 1994
    ..These observations provide insights into the roles performed by BRF in Pol III transcription complex assembly...
  35. ncbi request reprint hnRNP K: an HDM2 target and transcriptional coactivator of p53 in response to DNA damage
    Abdeladim Moumen
    The Wellcome Trust and Cancer Research UK Gurdon Institute and Department of Zoology, Cambridge University, Tennis Court Road, Cambridge CB2 1QN, United Kingdom
    Cell 123:1065-78. 2005
    ..These findings establish hnRNP K as a new HDM2 target and show that, by serving as a cofactor for p53, hnRNP K plays key roles in coordinating transcriptional responses to DNA damage...
  36. ncbi request reprint Rapid PIKK-dependent release of Chk1 from chromatin promotes the DNA-damage checkpoint response
    Veronique A J Smits
    The Wellcome Trust and Cancer Research UK Gurdon Institute, Tennis Court Road, Cambridge CB2 1QN, United Kingdom
    Curr Biol 16:150-9. 2006
    ..Within these pathways, the effector kinase Chk1 plays a central role in mediating cell-cycle arrest in response to DNA damage, and it does so by phosphorylating key cell-cycle regulators...
  37. ncbi request reprint Structure of an Xrcc4-DNA ligase IV yeast ortholog complex reveals a novel BRCT interaction mode
    Andrew S Doré
    Department of Biochemistry, University of Cambridge, 80 Tennis Court Road, Cambridge, CB2 1GA, UK
    DNA Repair (Amst) 5:362-8. 2006
    ..The structure reveals a novel mode of protein recognition by a tandem BRCT repeat, and in addition provides a molecular basis for a human LIG4 syndrome clinical condition...
  38. ncbi request reprint XLF interacts with the XRCC4-DNA ligase IV complex to promote DNA nonhomologous end-joining
    Peter Ahnesorg
    The Gurdon Institute, Cambridge University, UK
    Cell 124:301-13. 2006
    ..XLF thus constitutes a novel core component of the mammalian NHEJ apparatus...
  39. ncbi request reprint Suppression of HIV-1 infection by a small molecule inhibitor of the ATM kinase
    Alan Lau
    KuDOS Pharmaceuticals Limited, 327 Cambridge Science Park, Milton Road, Cambridge CB4 0WG, UK
    Nat Cell Biol 7:493-500. 2005
    ..Consistent with these observations, we demonstrate that a novel and specific small molecule inhibitor of ATM kinase activity, KU-55933, is capable of suppressing the replication of both wild-type and drug-resistant HIV-1...
  40. ncbi request reprint Targeting the DNA repair defect in BRCA mutant cells as a therapeutic strategy
    Hannah Farmer
    Cancer Research UK Gene Function and Regulation Group, London, UK
    Nature 434:917-21. 2005
    ..These results illustrate how different pathways cooperate to repair damage, and suggest that the targeted inhibition of particular DNA repair pathways may allow the design of specific and less toxic therapies for cancer...
  41. pmc Saccharomyces cerevisiae histone H2A Ser122 facilitates DNA repair
    Anne C Harvey
    Department of Biochemistry, University of Cambridge, UK
    Genetics 170:543-53. 2005
    ....
  42. ncbi request reprint Conserved modes of recruitment of ATM, ATR and DNA-PKcs to sites of DNA damage
    Jacob Falck
    The Wellcome Trust and Cancer Research UK Gurdon Institute, and Department of Zoology, Cambridge University, Tennis Court Road, Cambridge CB2 1QN, UK
    Nature 434:605-11. 2005
    ....
  43. ncbi request reprint Yeast Nhp6A/B and mammalian Hmgb1 facilitate the maintenance of genome stability
    Sabrina Giavara
    The Wellcome Trust and Cancer Research UK Gurdon Institute and Department of Zoology, University of Cambridge, Tennis Court Road, Cambridge CB2 1QN, United Kingdom
    Curr Biol 15:68-72. 2005
    ..Taken together, these data indicate that Nhp6A/B and Hmgb1 protect DNA from damaging agents and thus guard against the generation of genomic aberrations...
  44. ncbi request reprint Specific association of mouse MDC1/NFBD1 with NBS1 at sites of DNA-damage
    Alicia C Lee
    Experimental Immunology Branch, National Cancer Institute, National Institutes of Health, Bethesda, Maryland, USA
    Cell Cycle 4:177-82. 2005
    ..These data suggest that mMDC1 is a marker for both exogenously and endogenously generated DNA double-stranded breaks and that its interaction with the MRN complex is initiated exclusively by DNA damage...
  45. ncbi request reprint Binding of chromatin-modifying activities to phosphorylated histone H2A at DNA damage sites
    Jessica A Downs
    The Wellcome Trust Cancer Research UK Gurdon Institute and Department of Zoology, University of Cambridge, Tennis Court Road, Cambridge, CB2 1QR, United Kingdom
    Mol Cell 16:979-90. 2004
    ..Thus, phosphorylation of H2A at DNA damage sites creates a mark recognized by different chromatin modifiers. This interaction leads to stepwise chromatin reconfiguration, allowing efficient DNA repair...
  46. ncbi request reprint gammaH2AX and MDC1: anchoring the DNA-damage-response machinery to broken chromosomes
    Manuel Stucki
    Institute of Veterinary Biochemistry and Molecular Biology, University of Zurich, Winterthurerstrasse 190, 8057 Zurich, Switzerland
    DNA Repair (Amst) 5:534-43. 2006
    ....
  47. ncbi request reprint Double-strand breaks trigger MRX- and Mec1-dependent, but Tel1-independent, checkpoint activation
    Muriel Grenon
    Wellcome Trust and Cancer Research UK Gurdon Institute, Cambridge UK
    FEMS Yeast Res 6:836-47. 2006
    ..The existence of this novel mode of checkpoint activation explains why several previous studies had reported that mutations in the MRX complex did not abrogate DSB-induced checkpoint activation in asynchronous cells...
  48. pmc Distinct roles of chromatin-associated proteins MDC1 and 53BP1 in mammalian double-strand break repair
    Anyong Xie
    Department of Medicine, Harvard Medical School and Beth Israel Deaconess Medical Center, 330 Brookline Avenue, Boston, MA 02215, USA
    Mol Cell 28:1045-57. 2007
    ..These results suggest a specialized adaptation of the "histone code" in which distinct histone tail-protein interactions promote engagement of distinct DSBR pathways...
  49. pmc Crystal structure of human XLF/Cernunnos reveals unexpected differences from XRCC4 with implications for NHEJ
    Yi Li
    Department of Biochemistry, University of Cambridge, Cambridge, UK
    EMBO J 27:290-300. 2008
    ..Our data are most consistent with head-to-head interactions in a 2:2:1 XRCC4:XLF:Ligase IV complex...
  50. pmc Orchestration of the DNA-damage response by the RNF8 ubiquitin ligase
    Nadine K Kolas
    Samuel Lunenfeld Research Institute, Mount Sinai Hospital, 600 University Avenue, Toronto M5G1X5, Ontario, Canada
    Science 318:1637-40. 2007
    ..These results demonstrate how the DNA-damage response is orchestrated by ATM-dependent phosphorylation of MDC1 and RNF8-mediated ubiquitination...
  51. pmc Human CtIP promotes DNA end resection
    Alessandro A Sartori
    The Wellcome Trust and Cancer Research UK Gurdon Institute, and Department of Zoology, University of Cambridge, Tennis Court Road, Cambridge CB2 1QN, UK
    Nature 450:509-14. 2007
    ..These findings establish evolutionarily conserved roles for CtIP-like proteins in controlling DSB resection, checkpoint signalling and homologous recombination...
  52. pmc Rad9 BRCT domain interaction with phosphorylated H2AX regulates the G1 checkpoint in budding yeast
    Andrew Hammet
    Wellcome Trust and Cancer Research UK Gurdon Institute and Department of Zoology, University of Cambridge, Tennis Court Road, Cambridge CB2 1QN, UK
    EMBO Rep 8:851-7. 2007
    ..These findings indicate that constitutive Tudor domain-mediated and damage-specific BRCT domain-phospho-H2A-dependent interactions of Rad9 with chromatin cooperate to establish G1 checkpoint arrest...
  53. pmc Spreading of mammalian DNA-damage response factors studied by ChIP-chip at damaged telomeres
    Andreas Meier
    The Wellcome Trust and Cancer Research UK Gurdon Institute, Department of Zoology, University of Cambridge, Cambridge, UK
    EMBO J 26:2707-18. 2007
    ..Finally, we observe weak gammaH2AX signals at telomeres of proliferating cells, but not in hTERT immortalised cells, suggesting that low telomerase activity leads to telomere uncapping and senescence in proliferating primary cells...
  54. pmc Distinct domains in Nbs1 regulate irradiation-induced checkpoints and apoptosis
    Simone Difilippantonio
    Experimental Immunology Branch and 3Genetics Branch, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA
    J Exp Med 204:1003-11. 2007
    ..However, the C-terminal region regulates irradiation-induced apoptosis. These studies provide insight into the complex interplay between Nbs1 and ATM in the DNA damage response...
  55. ncbi request reprint Regulation of histone H3 lysine 56 acetylation in Schizosaccharomyces pombe
    Blerta Xhemalce
    Unite de la Dynamique du Génome, Institut Pasteur, 25 rue du Dr Roux, 75724, Paris Cedex 15, France
    J Biol Chem 282:15040-7. 2007
    ....
  56. pmc Yeast Rtt109 promotes genome stability by acetylating histone H3 on lysine 56
    Robert Driscoll
    Wellcome Trust and Cancer Research U K Gurdon Institute and the Department of Zoology, University of Cambridge, Tennis Court Road, Cambridge CB2 1QN, UK
    Science 315:649-52. 2007
    ..These data establish Rtt109p as a member of a new class of histone acetyltransferases and show that its actions are critical for cell survival in the presence of DNA damage during S phase...
  57. pmc The non-homologous end-joining protein Nej1p is a target of the DNA damage checkpoint
    Peter Ahnesorg
    Wellcome Trust and Cancer Research UK Gurdon Institute, University of Cambridge, Tennis Court Road, Cambridge CB2 1QN, United Kingdom
    DNA Repair (Amst) 6:190-201. 2007
    ....
  58. ncbi request reprint EDD mediates DNA damage-induced activation of CHK2
    Michelle J Henderson
    Cancer Research Program, Garvan Institute of Medical Research, 384 Victoria St, Darlinghurst, New South Wales 2010, Australia
    J Biol Chem 281:39990-40000. 2006
    ..These results identify EDD as a novel mediator in DNA damage signal transduction via CHK2 and emphasize the potential importance of EDD in cancer...
  59. ncbi request reprint Evolutionary and functional conservation of the DNA non-homologous end-joining protein, XLF/Cernunnos
    Pierre Hentges
    Genome Damage and Stability Centre, University of Sussex, Brighton BN1 9RQ, United Kingdom
    J Biol Chem 281:37517-26. 2006
    ..We therefore conclude that XLF family proteins interact with the ligase IV-XRCC4 complex to constitute the evolutionarily conserved enzymatic core of the NHEJ machinery...
  60. pmc CDK targets Sae2 to control DNA-end resection and homologous recombination
    Pablo Huertas
    The Wellcome Trust and Cancer Research UK Gurdon Institute, and Department of Zoology, University of Cambridge, Tennis Court Road, Cambridge CB2 1QN, UK
    Nature 455:689-92. 2008
    ..These findings therefore provide a mechanistic basis for cell-cycle control of DSB repair and highlight the importance of regulating DSB resection...
  61. ncbi request reprint Identification and characterization of a novel and specific inhibitor of the ataxia-telangiectasia mutated kinase ATM
    Ian Hickson
    KuDOS Pharmaceuticals Ltd, Cambridge Science Park, Milton Road, Cambridge, UK
    Cancer Res 64:9152-9. 2004
    ..We conclude that KU-55933 is a novel, specific, and potent inhibitor of the ATM kinase...
  62. ncbi request reprint A pathway of double-strand break rejoining dependent upon ATM, Artemis, and proteins locating to gamma-H2AX foci
    Enriqueta Riballo
    Genome Damage and Stability Centre, University of Sussex, East Sussex, BN1 9RQ, United Kingdom
    Mol Cell 16:715-24. 2004
    ..The significant radiosensitivity of Artemis-deficient cells demonstrates the importance of this component of DSB repair to survival...
  63. ncbi request reprint Suppression of homologous recombination by the Saccharomyces cerevisiae linker histone
    Jessica A Downs
    The Wellcome Trust Cancer Research UK Institute, University of Cambridge, Tennis Court Road, Cambridge CB2 1QR, United Kingdom
    Mol Cell 11:1685-92. 2003
    ..Finally, we show that Hho1p is inhibitory to the recombination-dependent mechanism of telomere maintenance. The role of linker histones in genome stability, aging, and tumorigenesis is discussed...
  64. ncbi request reprint ATM and ATR
    Jane M Bradbury
    The Wellcome Trust Cancer Research UK Institute, Tennis Court Road, Cambridge CB2 1QR, UK
    Curr Biol 13:R468. 2003
  65. ncbi request reprint MDC1 is required for the intra-S-phase DNA damage checkpoint
    Michal Goldberg
    The Wellcome Trust Cancer Research UK Institute of Cancer and Developmental Biology and Department of Zoology, University of Cambridge, Cambridge CB2 1QR, UK
    Nature 421:952-6. 2003
    ..These findings reveal that MDC1-mediated focus formation by the MRE11 complex at sites of DNA damage is crucial for the efficient activation of the intra-S-phase checkpoint...
  66. ncbi request reprint A heterotrimeric PCNA in the hyperthermophilic archaeon Sulfolobus solfataricus
    Isabelle Dionne
    Medical Research Council Cancer Cell Unit, Hutchison MRC Research Centre, Hills Road, Cambridge CB2 2XZ, United Kingdom
    Mol Cell 11:275-82. 2003
    ..This provides a mechanism to tightly couple DNA synthesis and Okazaki fragment maturation. Additionally, unique subunit-specific interactions between components of the clamp loader, RFC, suggest a model for clamp loading of PCNA...
  67. pmc The Gam protein of bacteriophage Mu is an orthologue of eukaryotic Ku
    Fabrizio d'Adda di Fagagna
    The Wellcome Trust Cancer Research UK Institute of Cancer and Developmental Biology, University of Cambridge, Tennis Court Road, UK
    EMBO Rep 4:47-52. 2003
    ..These data reveal structural and functional parallels between bacteriophage Gam and eukaryotic Ku and suggest that their functions have been evolutionarily conserved...
  68. ncbi request reprint Identification of a DNA nonhomologous end-joining complex in bacteria
    Geoffrey R Weller
    Cambridge Institute for Medical Research and Department of Haematology, University of Cambridge, Hills Road, Cambridge CB2 2XY, UK
    Science 297:1686-9. 2002
    ....
  69. ncbi request reprint Interfaces between the detection, signaling, and repair of DNA damage
    John Rouse
    The Wellcome Trust Cancer Research UK Institute of Cancer and Developmental Biology, University of Cambridge, Tennis Court Road, Cambridge CB2 1QR, UK
    Science 297:547-51. 2002
    ..In contrast, it is still unclear how primary DNA damage is detected and how this interfaces with signal transduction and DNA repair proteins...
  70. ncbi request reprint Structural basis for the NAD-dependent deacetylase mechanism of Sir2
    Jeong Ho Chang
    National Creative Research Initiative Center for Structural Biology and Department of Life Science, Pohang University of Science and Technology, Hyo ja dong, San31, Pohang, Kyungbook 790 784, South Korea
    J Biol Chem 277:34489-98. 2002
    ..The crystal structures of wild type and mutant derivatives of Sir2, in conjunction with biochemical analyses of the mutants, provide novel insights into the reaction mechanism of Sir2-mediated deacetylation...
  71. ncbi request reprint Structural and functional versatility of the FHA domain in DNA-damage signaling by the tumor suppressor kinase Chk2
    Jiejin Li
    Division of Protein Structure, National Institute for Medical Research, The Ridgeway, Mill Hill, London, United Kingdom
    Mol Cell 9:1045-54. 2002
    ..Although phospho-dependent binding is important for Chk2 activity, previously uncharacterized phospho-independent FHA domain interactions appear to be the primary target of oncogenic lesions...
  72. ncbi request reprint The Mre11 complex: at the crossroads of dna repair and checkpoint signalling
    Damien D'Amours
    Wellcome Trust and Cancer Research, UK Institute of Cancer and Developmental Biology, University of Cambridge, Tennis Court Road, Cambridge CB2 1QR, UK
    Nat Rev Mol Cell Biol 3:317-27. 2002
    ..The molecular defect that underlies these phenotypes has long been thought to be related to a DNA repair deficiency. However, recent studies have uncovered functions for the Mre11 complex in checkpoint signalling and DNA replication...
  73. ncbi request reprint Lcd1p recruits Mec1p to DNA lesions in vitro and in vivo
    John Rouse
    Wellcome Trust and Cancer Research UK, Institute of Cancer and Developmental Biology, University of Cambridge, Cambridge CB2 1QR, United Kingdom
    Mol Cell 9:857-69. 2002
    ..Recruitment of Lcd1p to these lesions is independent of Mec1p and Rad9p/Rad24p. Thus, recruitment of Mec1p to DNA lesions by Lcd1p is crucial for the DNA damage response...
  74. ncbi request reprint The interaction of Alba, a conserved archaeal chromatin protein, with Sir2 and its regulation by acetylation
    Stephen D Bell
    Medical Research Council MRC Cancer Cell Unit, The Hutchison MRC Research Centre, Hills Road, Cambridge, CB2 2QH, UK
    Science 296:148-51. 2002
    ..These data provide a paradigm for how Sir2 family proteins influence transcription and suggest that modulation of chromatin structure by acetylation arose before the divergence of the archaeal and eukaryotic lineages...
  75. ncbi request reprint Cancer: protective packaging for DNA
    Jessica A Downs
    Nature 424:732-4. 2003
  76. ncbi request reprint Increased genome instability in aging yeast
    Serge Gravel
    The Wellcome Trust Cancer Research, UK
    Cell 115:1-2. 2003
    ..Furthermore, they show that this reflects an impaired ability to correctly detect and repair DNA double-strand breaks. These results provide insights into how aging can engender genomic instability in eukaryotic cells...
  77. ncbi request reprint Tudor domains track down DNA breaks
    Manuel Stucki
    Nat Cell Biol 6:1150-2. 2004
  78. ncbi request reprint p53 prevents the accumulation of double-strand DNA breaks at stalled-replication forks induced by UV in human cells
    Shoshana Squires
    Department of Zoology, University of Cambridge, Cambridge, UK
    Cell Cycle 3:1543-57. 2004
    ..We propose that a major mechanism by which p53 maintains genome stability is the prevention of DSB accumulation at long-lived ssDNA regions in stalled-replication forks...
  79. pmc Suppression of retroviral infection by the RAD52 DNA repair protein
    Alan Lau
    KuDOS Pharmaceuticals Limited, 327 Cambridge Science Park, Cambridge, UK
    EMBO J 23:3421-9. 2004
    ..Instead, the mechanism of attenuation of retroviral infection by RAD52 appears to be based upon competition between the RAD52 protein and active integration complexes for the retroviral cDNA genome...
  80. ncbi request reprint Functional links between telomeres and proteins of the DNA-damage response
    Fabrizio d'Adda di Fagagna
    IFOM Foundation The FIRC Institute of Molecular Oncology Foundation, 20139 Milan, Italy
    Genes Dev 18:1781-99. 2004
    ..In this review, we discuss the current knowledge of both the telomere and the DDR, and then propose an integrated model for the events associated with the metabolism of DNA ends in these two distinct physiological contexts...
  81. ncbi request reprint MDC1/NFBD1: a key regulator of the DNA damage response in higher eukaryotes
    Manuel Stucki
    Department of Zoology, The Wellcome Trust Cancer Research UK, Gurdon Institute of Cancer and Developmental Biology, University of Cambridge, Tennis Court Road, Cambridge CB2 1QR, UK
    DNA Repair (Amst) 3:953-7. 2004
    ..Thus, MDC1/NFBD1 appears to be a key regulator of the DNA damage response in mammalian cells...
  82. pmc Mdc1 couples DNA double-strand break recognition by Nbs1 with its H2AX-dependent chromatin retention
    Claudia Lukas
    Danish Cancer Society, Institute of Cancer Biology, Copenhagen, Denmark
    EMBO J 23:2674-83. 2004
    ....
  83. ncbi request reprint A means to a DNA end: the many roles of Ku
    Jessica A Downs
    Department of Biochemistry, University of Cambridge, 80 Tennis Court Road, Cambridge CB2 1GA, UK
    Nat Rev Mol Cell Biol 5:367-78. 2004
  84. ncbi request reprint Activation of the DNA damage response by telomere attrition: a passage to cellular senescence
    Philip M Reaper
    The Wellcome Trust Cancer Research UK Gurdon Institute, University of Cambridge, UK
    Cell Cycle 3:543-6. 2004
    ..We consider the identities of the key DNA damage response factors involved in senescence and discuss a model for the molecular events occurring in pre-senescent cells that ultimately lead to a permanent cell cycle arrest phenotype...
  85. pmc Saccharomyces cerevisiae Sin3p facilitates DNA double-strand break repair
    Ali Jazayeri
    Wellcome Trust Cancer Research UK Gurdon Institute and Department of Zoology, University of Cambridge, Tennis Court Road, Cambridge CB2 1QR, United Kingdom
    Proc Natl Acad Sci U S A 101:1644-9. 2004
    ..Taken together, these results define a role for the Sin3p/Rpd3p complex in the modulation of DNA repair...
  86. ncbi request reprint A DNA damage checkpoint response in telomere-initiated senescence
    Fabrizio d'Adda di Fagagna
    1 The Wellcome Trust Cancer Research UK Institute of Cancer and Developmental Biology, University of Cambridge, Cambridge CB2 1QR, UK 2 Present address IFOM FIRC Institute of Molecular Oncology, Via Adamello 16, 20139 Milan, Italy
    Nature 426:194-8. 2003
    ..Thus, we propose that telomere-initiated senescence reflects a DNA damage checkpoint response that is activated with a direct contribution from dysfunctional telomeres...
  87. ncbi request reprint Separation-of-function mutants of yeast Ku80 reveal a Yku80p-Sir4p interaction involved in telomeric silencing
    Rajat Roy
    Wellcome Trust Cancer Research UK Institute of Cancer and Developmental Biology, and Deparment of Zoology, University of Cambridge, Tennis Court Road, Cambridge CB2 1QR, UK
    J Biol Chem 279:86-94. 2004
    ..Taken together with other data, these findings indicate that the Yku80p-Sir4p interaction plays a vital role in the assembly of telomeric heterochromatin...
  88. ncbi request reprint The FHA domain
    Daniel Durocher
    Samuel Lumenfeld Research Institute, Mount Sinai Hospital, Toronto, ON, Canada M5G 1 X5
    FEBS Lett 513:58-66. 2002
    ..It is also found in a number of bacterial proteins. This suggests that FHA domain-mediated phospho-dependent assembly of protein complexes is an ancient and widespread regulatory mechanism...
  89. pmc Molecular cross-talk among chromosome fragility syndromes
    Jordi Surralles
    Group of Mutagenesis, Department of Genetics and Microbiology, Universitat Autonoma de Barcelona, 08193 Bellaterra, Barcelona, Spain
    Genes Dev 18:1359-70. 2004