Stephen C Hyde

Summary

Affiliation: University of Oxford
Country: UK

Publications

  1. doi request reprint Ignoring the nonsense: a phase II trial in cystic fibrosis
    Stephen C Hyde
    UK Cystic Fibrosis Gene Therapy Consortium and Nuffield Department of Clinical Laboratory Sciences, John Radcliffe Hospital, Oxford OX3 9DU, UK
    Lancet 372:691-2. 2008
  2. doi request reprint CpG-free plasmids confer reduced inflammation and sustained pulmonary gene expression
    Stephen C Hyde
    Gene Medicine Research Group, Nuffield Department of Clinical Laboratory Sciences, University of Oxford, Oxford OX3 9DU, UK
    Nat Biotechnol 26:549-51. 2008
  3. ncbi request reprint Strategies for long-term expression of transgenes in the respiratory epithelium
    Deborah R Gill
    University of Oxford, Gene Medicine Research Group, Nuffield Department of Clinical Laboratory Sciences, John Radcliffe Hospital, Headington, Oxford, OX3 9DU, UK
    Curr Opin Mol Ther 12:386-93. 2010
  4. doi request reprint CpG-free plasmid expression cassettes for cystic fibrosis gene therapy
    Ian A Pringle
    Gene Medicine Research Group, NDCLS, John Radcliffe Hospital, University of Oxford, Oxford OX3 9DU, UK
    Biomaterials 33:6833-42. 2012
  5. pmc Inefficient cationic lipid-mediated siRNA and antisense oligonucleotide transfer to airway epithelial cells in vivo
    Uta Griesenbach
    Department of Gene Therapy, Faculty of Medicine, National Heart and Lung Institute, Imperial College, London, UK
    Respir Res 7:26. 2006
  6. ncbi request reprint Enhanced lung gene expression after aerosol delivery of concentrated pDNA/PEI complexes
    Lee A Davies
    Nuffield Department of Clinical Laboratory Sciences, University of Oxford, John Radcliffe Hospital, Oxford, UK
    Mol Ther 16:1283-90. 2008
  7. doi request reprint The use of CpG-free plasmids to mediate persistent gene expression following repeated aerosol delivery of pDNA/PEI complexes
    Lee A Davies
    Gene Medicine Research Group, Nuffield Department of Clinical Laboratory Sciences, John Radcliffe Hospital, University of Oxford, Oxford, UK
    Biomaterials 33:5618-27. 2012
  8. doi request reprint Rapid identification of novel functional promoters for gene therapy
    Ian A Pringle
    GeneMedicine Research Group, NDCLS, John Radcliffe Hospital, University of Oxford, OX3 9DU Oxford, UK
    J Mol Med (Berl) 90:1487-96. 2012
  9. doi request reprint Adenovirus-mediated in utero expression of CFTR does not improve survival of CFTR knockout mice
    Lee A Davies
    Nuffield Department of Clinical Laboratory Sciences, John Radcliffe Hospital, University of Oxford, Oxford, UK
    Mol Ther 16:812-8. 2008
  10. ncbi request reprint Electroporation enhances reporter gene expression following delivery of naked plasmid DNA to the lung
    Ian A Pringle
    GeneMedicine Research Group, Nuffield Department of Clinical Laboratory Sciences, University of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, UK
    J Gene Med 9:369-80. 2007

Collaborators

  • Eric W F W Alton
  • Gerry McLachlan
  • Deborah R Gill
  • Syahril Abdullah
  • C M Gordon
  • Ralph A Schmid
  • David N Sheppard
  • U Griesenbach
  • J C Davies
  • N S Yew
  • Margarida D Amaral
  • N J Caplen
  • Stephen Tate
  • Felix M Munkonge
  • Lee A Davies
  • Ian A Pringle
  • Stephanie G Sumner-Jones
  • Anna E Lawton
  • Seng H Cheng
  • Graciela Nunez-Alonso
  • David J Porteous
  • Reto P Bazzani
  • Graciela A Nunez-Alonso
  • Anusha Varathalingam
  • David D S Collie
  • Alison Baker
  • Mary M Connolly
  • Henry L Hebel
  • Peter Tennant
  • Uz Stammberger
  • Heather Davidson
  • Amiq Gazdhar
  • Reza Tavakoli
  • Mathias Gugger
  • A Christopher Boyd
  • Michael Emerson
  • Bihui Xu
  • Ronald K Scheule
  • Rebekka Harding-Smith
  • Dominique McCormick
  • Thomas C Roberts
  • Anne Marie Hewitt
  • Ying Cai
  • Ron K Scheule
  • Hazel Painter
  • Christina Vrettou
  • Eilidh Baker
  • Mario Chan
  • David Ferguson
  • Jie Zhu
  • Claire Seguela
  • Richard Blundell
  • Abigail Wilson
  • Jaroslaw Pierog
  • Rebecca Coles
  • Michele Genoni
  • Deborah Penque
  • Gordon MacGregor
  • Murat Bilici
  • Jurg Hamacher
  • Ann Doherty
  • Lee Davies
  • Hazel A Painter
  • Anna Bogdanova
  • Alistair Pirrie
  • Neville Davies
  • Kevin Hannavy
  • Ronald A Coffee
  • Louise Renwick
  • Susan Rhind
  • D David S Collie
  • Elspeth Milne
  • Hazel Alsop Painter

Detail Information

Publications22

  1. doi request reprint Ignoring the nonsense: a phase II trial in cystic fibrosis
    Stephen C Hyde
    UK Cystic Fibrosis Gene Therapy Consortium and Nuffield Department of Clinical Laboratory Sciences, John Radcliffe Hospital, Oxford OX3 9DU, UK
    Lancet 372:691-2. 2008
  2. doi request reprint CpG-free plasmids confer reduced inflammation and sustained pulmonary gene expression
    Stephen C Hyde
    Gene Medicine Research Group, Nuffield Department of Clinical Laboratory Sciences, University of Oxford, Oxford OX3 9DU, UK
    Nat Biotechnol 26:549-51. 2008
    ..Using a CpG-free pDNA expression vector, we achieved sustained (>or=56 d) in vivo transgene expression in the absence of lung inflammation...
  3. ncbi request reprint Strategies for long-term expression of transgenes in the respiratory epithelium
    Deborah R Gill
    University of Oxford, Gene Medicine Research Group, Nuffield Department of Clinical Laboratory Sciences, John Radcliffe Hospital, Headington, Oxford, OX3 9DU, UK
    Curr Opin Mol Ther 12:386-93. 2010
    ..This review describes recent developments in achieving persistent transgene expression in the airways by specifically targeting the cells of the respiratory epithelium lining the nose and lung...
  4. doi request reprint CpG-free plasmid expression cassettes for cystic fibrosis gene therapy
    Ian A Pringle
    Gene Medicine Research Group, NDCLS, John Radcliffe Hospital, University of Oxford, Oxford OX3 9DU, UK
    Biomaterials 33:6833-42. 2012
    ..Systematic evaluation of each functional component of the plasmid has resulted in an improved design, exhibiting superior levels and duration of lung gene expression...
  5. pmc Inefficient cationic lipid-mediated siRNA and antisense oligonucleotide transfer to airway epithelial cells in vivo
    Uta Griesenbach
    Department of Gene Therapy, Faculty of Medicine, National Heart and Lung Institute, Imperial College, London, UK
    Respir Res 7:26. 2006
    ..The cationic lipid Genzyme lipid (GL) 67 is the current "gold-standard" for in vivo lung gene transfer. Here, we assessed, if GL67 mediated uptake of siRNAs and asODNs into airway epithelium in vivo...
  6. ncbi request reprint Enhanced lung gene expression after aerosol delivery of concentrated pDNA/PEI complexes
    Lee A Davies
    Nuffield Department of Clinical Laboratory Sciences, University of Oxford, John Radcliffe Hospital, Oxford, UK
    Mol Ther 16:1283-90. 2008
    ..It seems likely that similar benefits might be achievable with alternative delivery routes and with other nonviral formulations...
  7. doi request reprint The use of CpG-free plasmids to mediate persistent gene expression following repeated aerosol delivery of pDNA/PEI complexes
    Lee A Davies
    Gene Medicine Research Group, Nuffield Department of Clinical Laboratory Sciences, John Radcliffe Hospital, University of Oxford, Oxford, UK
    Biomaterials 33:5618-27. 2012
    ..Together these data indicate that pDNA/PEI aerosol formulations offer a versatile platform for gene delivery to the lung resulting in sustained transgene expression suitable for treatment of chronic lung diseases...
  8. doi request reprint Rapid identification of novel functional promoters for gene therapy
    Ian A Pringle
    GeneMedicine Research Group, NDCLS, John Radcliffe Hospital, University of Oxford, OX3 9DU Oxford, UK
    J Mol Med (Berl) 90:1487-96. 2012
    ..Furthermore, the database search criteria can be quickly adjusted to identify other novel promoter elements for a variety of transgene expression applications...
  9. doi request reprint Adenovirus-mediated in utero expression of CFTR does not improve survival of CFTR knockout mice
    Lee A Davies
    Nuffield Department of Clinical Laboratory Sciences, John Radcliffe Hospital, University of Oxford, Oxford, UK
    Mol Ther 16:812-8. 2008
    ....
  10. ncbi request reprint Electroporation enhances reporter gene expression following delivery of naked plasmid DNA to the lung
    Ian A Pringle
    GeneMedicine Research Group, Nuffield Department of Clinical Laboratory Sciences, University of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, UK
    J Gene Med 9:369-80. 2007
    ..Electroporation has been used to enhance gene transfer in a range of tissues. Here we assess the usefulness of electroporation for enhancing gene transfer in the lungs of mice and sheep...
  11. doi request reprint A novel mixing device for the reproducible generation of nonviral gene therapy formulations
    Lee A Davies
    Gene Medicine Group, Nuffield Department of Clinical Laboratory Sciences, University of Oxford, John Radcliffe Hospital, Oxford, UK
    Biotechniques 49:666-8. 2010
    ..Here we describe a simple pneumatic mixing device that enables the rapid and reproducible production of large volumes of nonviral gene therapy formulations and demonstrate its suitability for use with shear-sensitive pDNA...
  12. doi request reprint The significance of plasmid DNA preparations contaminated with bacterial genomic DNA on inflammatory responses following delivery of lipoplexes to the murine lung
    Reto P Bazzani
    Gene Medicine Research Group, NDCLS, John Radcliffe Hospital, University of Oxford, Oxford OX3 9DU, UK
    Biomaterials 32:9854-65. 2011
    ..These studies help define the acceptable limit of genomic DNA contamination that will impact FDA/EMEA regulatory guidelines defining clinical grade purity of plasmid DNA for human use in gene therapy and vaccination studies...
  13. pmc Lack of repeat transduction by recombinant adeno-associated virus type 5/5 vectors in the mouse airway
    Stephanie G Sumner-Jones
    Nuffield Department of Clinical Laboratory Sciences, John Radcliffe Hospital, University of Oxford, Oxford, UK
    J Virol 81:12360-7. 2007
    ..These results suggest that without the development of an effective and clinically acceptable immunosuppression strategy, treatments for chronic diseases that require repeated administration of rAAV5/5 vectors will be unsuccessful...
  14. ncbi request reprint Identification of transfected cell types following non-viral gene transfer to the murine lung
    Lee A Davies
    Gene Medicine Research Group, University of Oxford, John Radcliffe Hospital, Oxford, UK
    J Gene Med 9:184-96. 2007
    ..We have investigated the use of the green fluorescent protein (GFP) to identify transfected cells in a mouse lung model...
  15. ncbi request reprint Aerosol Delivery of DNA/Liposomes to the Lung for Cystic Fibrosis Gene Therapy
    Lee A Davies
    1 Gene Medicine Research Group, Nuffield Division of Clinical Laboratory Sciences, University of Oxford, John Radcliffe Hospital, Oxford, OX3 9DU, United Kingdom
    Hum Gene Ther Clin Dev 25:97-107. 2014
    ..On the basis of these results, we have selected the AeroEclipse II nebulizer for the delivery of pDNA/GL67A formulations to the lungs of CF patients as part of phase IIa/b clinical studies. ..
  16. ncbi request reprint Electrohydrodynamic comminution: a novel technique for the aerosolisation of plasmid DNA
    Lee A Davies
    GeneMedicine Research Group, Nuffield Department of Clinical Laboratory Sciences, University of Oxford, John Radcliffe Hospital, Oxford, OX3 9DU, UK
    Pharm Res 22:1294-304. 2005
    ..This study investigated the viability of an alternative nebulisation technique, electrohydrodynamic (EHD) comminution for the aerosol delivery of naked DNA in vivo...
  17. doi request reprint Assessment of CFTR function after gene transfer in vitro and in vivo
    Uta Griesenbach
    Department of Gene Therapy, Faculty of Medicine, Imperial College London, UK
    Methods Mol Biol 433:229-42. 2008
    ..Here, we describe pre-clinical methods related to assessing correction of the CF chloride transport defect...
  18. ncbi request reprint Optimizing aerosol gene delivery and expression in the ovine lung
    Gerry McLachlan
    Medical Genetics Section, School of Molecular and Clinical Medicine, University of Edinburgh, Western General Hospital, Edinburgh, UK
    Mol Ther 15:348-54. 2007
    ..Dose-related toxicity of GTA was reduced by aerosol administration compared to direct instillation. This large animal model will allow us to move toward clinical studies with greater confidence...
  19. ncbi request reprint Prolonged amelioration of acute lung allograft rejection by overexpression of human interleukin-10 under control of a long acting ubiquitin C promoter in rats
    Uz Stammberger
    Division of General Thoracic Surgery, University Hospital, Bern, Switzerland
    J Heart Lung Transplant 25:1474-9. 2006
    ..The prolonged effect of electroporation-mediated human interleukin-10 (hIL-10) overexpression in skeletal muscle under the control of the constitutional polyubiquitin C promoter (pUb hIL-10) on rat lung allograft rejection was evaluated...
  20. ncbi request reprint Human-specific cystic fibrosis transmembrane conductance regulator antibodies detect in vivo gene transfer to ovine airways
    Heather Davidson
    Medical Sciences Medical Genetics, University of Edinburgh, Molecular Medicine Centre, Western General Hospital, Edinburgh EH4 2XU, United Kingdom
    Am J Respir Cell Mol Biol 35:72-83. 2006
    ..The strategic use of hCFTR-specific antibodies supports the utility of the normal sheep as a model for hCFTR gene transfer studies...
  21. ncbi request reprint Electroporation-mediated interleukin-10 overexpression in skeletal muscle reduces acute rejection in rat cardiac allografts
    Reza Tavakoli
    Division of Cardiac Surgery, Triemli Hospital, Zurich, Switzerland
    J Gene Med 8:242-8. 2006
    ..This study was designed to investigate the effect of electroporation-mediated overexpression of hIL-10 on acute rejection of cardiac allografts in the rat...
  22. ncbi request reprint Transfection efficiency and toxicity following delivery of naked plasmid DNA and cationic lipid-DNA complexes to ovine lung segments
    Michael Emerson
    Medical Genetics Section, School of Molecular and Clinical Medicine, and Department of Veterinary Pathology, College of Medicine and Veterinary Medicine, University of Edinburg, Edinburgh EH8 9AG, United Kingdom
    Mol Ther 8:646-53. 2003
    ..The severity of the inflammatory response appeared to correlate with the administered dose of DNA and was generally more severe for pDNA:GL67...