Paul Hunt

Summary

Affiliation: University of Edinburgh
Country: UK

Publications

  1. ncbi request reprint Numerous, robust genetic markers for Plasmodium chabaudi by the method of amplified fragment length polymorphism
    Katrina Grech
    University of Edinburgh, Division of Biological Sciences, ICAPB, Ashworth Laboratory, West Mains Road, Edinburgh EH9 3JT, UK
    Mol Biochem Parasitol 123:95-104. 2002
  2. ncbi request reprint Chloroquine resistance in Plasmodium chabaudi: are chloroquine-resistance transporter (crt) and multi-drug resistance (mdr1) orthologues involved?
    Paul Hunt
    Institute of Cell, Animal and Population Biology, University of Edinburgh, EH9 3JT, Scotland, UK
    Mol Biochem Parasitol 133:27-35. 2004
  3. pmc Artemisinin resistance in rodent malaria--mutation in the AP2 adaptor μ-chain suggests involvement of endocytosis and membrane protein trafficking
    Gisela Henriques
    Centro de Malaria and Doenças Tropicais, LA IHMT Universidade Nova de Lisboa, Lisbon, Portugal
    Malar J 12:118. 2013
  4. pmc Experimental evolution, genetic analysis and genome re-sequencing reveal the mutation conferring artemisinin resistance in an isogenic lineage of malaria parasites
    Paul Hunt
    Institute for Immunology and Infection Research, School of Biological Sciences, University of Edinburgh, Edinburgh, UK
    BMC Genomics 11:499. 2010
  5. pmc Gene encoding a deubiquitinating enzyme is mutated in artesunate- and chloroquine-resistant rodent malaria parasites
    Paul Hunt
    Institute for Immunology and Infection Research, School of Biological Sciences, University of Edinburgh, Ashworth Laboratory, Kings Buildings, Edinburgh EH9 3JT, UK
    Mol Microbiol 65:27-40. 2007
  6. ncbi request reprint Gene synteny and chloroquine resistance in Plasmodium chabaudi
    Paul Hunt
    Institute of Cell, Animal and Population Biology, University of Edinburgh, West Mains Road, Edinburgh EH9 3JT, Scotland, UK
    Mol Biochem Parasitol 136:157-64. 2004
  7. ncbi request reprint Estimating SNP proportions in populations of malaria parasites by sequencing: validation and applications
    Paul Hunt
    Institute for Immunity and Infection Research, School of Biological Science, University of Edinburgh, West Mains Road, Edinburgh EH9 3JT, Scotland, UK
    Mol Biochem Parasitol 143:173-82. 2005
  8. pmc Malaria genomics meets drug-resistance phenotyping in the field
    Paul Hunt
    Institute for Immunology and Infection Research, University of Edinburgh, Ashworth Laboratory, Kings Buildings, Edinburgh EH9 3JT, UK
    Genome Biol 10:314. 2009
  9. pmc Quantitative genome re-sequencing defines multiple mutations conferring chloroquine resistance in rodent malaria
    Katarzyna Kinga Modrzynska
    Institute for Immunology and Infection Research, University of Edinburgh, UK
    BMC Genomics 13:106. 2012
  10. pmc An AFLP-based genetic linkage map of Plasmodium chabaudi chabaudi
    Axel Martinelli
    Institute for Immunology and Infection Research, School of Biological Science, University of Edinburgh, Ashworth Laboratories, King s Buildings, West Mains Road, Edinburgh EH9 3JT, UK
    Malar J 4:11. 2005

Collaborators

Detail Information

Publications19

  1. ncbi request reprint Numerous, robust genetic markers for Plasmodium chabaudi by the method of amplified fragment length polymorphism
    Katrina Grech
    University of Edinburgh, Division of Biological Sciences, ICAPB, Ashworth Laboratory, West Mains Road, Edinburgh EH9 3JT, UK
    Mol Biochem Parasitol 123:95-104. 2002
    ..6 x 10(-4) per base pair. This is consistent with the sequence diversity found between alleles of candidate drug resistance genes from P. c. chabaudi clones AS and AJ identified and sequenced in this laboratory...
  2. ncbi request reprint Chloroquine resistance in Plasmodium chabaudi: are chloroquine-resistance transporter (crt) and multi-drug resistance (mdr1) orthologues involved?
    Paul Hunt
    Institute of Cell, Animal and Population Biology, University of Edinburgh, EH9 3JT, Scotland, UK
    Mol Biochem Parasitol 133:27-35. 2004
    ..This showed that neither of these genes, nor genes closely linked to them, were determinants of the chloroquine resistance in the P. chabaudi mutants...
  3. pmc Artemisinin resistance in rodent malaria--mutation in the AP2 adaptor μ-chain suggests involvement of endocytosis and membrane protein trafficking
    Gisela Henriques
    Centro de Malaria and Doenças Tropicais, LA IHMT Universidade Nova de Lisboa, Lisbon, Portugal
    Malar J 12:118. 2013
    ..Genetic markers of resistance are therefore required urgently. Previously, a mutation in a de-ubiquitinating enzyme was shown to confer artemisinin resistance in the rodent malaria parasite Plasmodium chabaudi...
  4. pmc Experimental evolution, genetic analysis and genome re-sequencing reveal the mutation conferring artemisinin resistance in an isogenic lineage of malaria parasites
    Paul Hunt
    Institute for Immunology and Infection Research, School of Biological Sciences, University of Edinburgh, Edinburgh, UK
    BMC Genomics 11:499. 2010
    ..Such genetic markers will further the investigation of resistance and its control in natural infections of the human malaria, P. falciparum...
  5. pmc Gene encoding a deubiquitinating enzyme is mutated in artesunate- and chloroquine-resistant rodent malaria parasites
    Paul Hunt
    Institute for Immunology and Infection Research, School of Biological Sciences, University of Edinburgh, Ashworth Laboratory, Kings Buildings, Edinburgh EH9 3JT, UK
    Mol Microbiol 65:27-40. 2007
    ..No mutations in the UBP-1 gene encoding the P. falciparum orthologue of the deubiquitinating enzyme were observed. The possible significance of these mutations in parasite responses to chloroquine or artemisinin is discussed...
  6. ncbi request reprint Gene synteny and chloroquine resistance in Plasmodium chabaudi
    Paul Hunt
    Institute of Cell, Animal and Population Biology, University of Edinburgh, West Mains Road, Edinburgh EH9 3JT, Scotland, UK
    Mol Biochem Parasitol 136:157-64. 2004
    ..The genetic order of the markers in P. chabaudi is co-linear with the physical linkage represented in the P. falciparum genome database. The findings provide evidence for extensive conservation of synteny between the two species...
  7. ncbi request reprint Estimating SNP proportions in populations of malaria parasites by sequencing: validation and applications
    Paul Hunt
    Institute for Immunity and Infection Research, School of Biological Science, University of Edinburgh, West Mains Road, Edinburgh EH9 3JT, Scotland, UK
    Mol Biochem Parasitol 143:173-82. 2005
    ..This technique can be used in the mapping of genetic loci underlying phenotypic traits and in the evaluation of the effects of different alleles upon the reproductive success (fitness) of parasites...
  8. pmc Malaria genomics meets drug-resistance phenotyping in the field
    Paul Hunt
    Institute for Immunology and Infection Research, University of Edinburgh, Ashworth Laboratory, Kings Buildings, Edinburgh EH9 3JT, UK
    Genome Biol 10:314. 2009
    ..A report of the 2nd Wellcome Trust Conference on Genomic Epidemiology of Malaria, Hinxton, UK, 14-17 June 2009...
  9. pmc Quantitative genome re-sequencing defines multiple mutations conferring chloroquine resistance in rodent malaria
    Katarzyna Kinga Modrzynska
    Institute for Immunology and Infection Research, University of Edinburgh, UK
    BMC Genomics 13:106. 2012
    ..chabaudi in which high level resistance to chloroquine has been progressively selected under laboratory conditions...
  10. pmc An AFLP-based genetic linkage map of Plasmodium chabaudi chabaudi
    Axel Martinelli
    Institute for Immunology and Infection Research, School of Biological Science, University of Edinburgh, Ashworth Laboratories, King s Buildings, West Mains Road, Edinburgh EH9 3JT, UK
    Malar J 4:11. 2005
    ..Despite the availability of some genome sequence data, an extensive genetic linkage map is needed for mapping the genes involved in certain traits...
  11. pmc Linkage group selection: rapid gene discovery in malaria parasites
    Richard Culleton
    Institute of Immunity and Infection Research, University of Edinburgh, Edinburgh EH9 3JT, United Kingdom
    Genome Res 15:92-7. 2005
    ..We show that molecular markers closely linked to dhfr, and only those linked to this gene, were reduced or removed by pyrimethamine treatment in accordance with the expectations of LGS...
  12. ncbi request reprint Amplified fragment length polymorphism measures proportions of malaria parasites carrying specific alleles in complex genetic mixtures
    Axel Martinelli
    University of Edinburgh, Division of Biological Sciences, ICAPB, Ashworth Laboratory, West Mains Road, Edinburgh EH9 3JT, UK
    Mol Biochem Parasitol 136:117-22. 2004
    ..The precision of the method is sufficient to detect reliably the effects of phenotypic selection at loci closely linked to a genetic locus under selection...
  13. pmc A genetic approach to the de novo identification of targets of strain-specific immunity in malaria parasites
    Axel Martinelli
    Institute of Immunology and Infection Research, Ashworth Laboratories, King s Buildings, University of Edinburgh, West Mains Road, Edinburgh EH9 3JT, United Kingdom
    Proc Natl Acad Sci U S A 102:814-9. 2005
    ..Another affected locus, unlinked to this selection valley, contained a member of the serine repeat antigen gene family...
  14. ncbi request reprint Validation of Pyrosequencing for accurate and high throughput estimation of allele frequencies in malaria parasites
    Sandra Cheesman
    Institute for Immunology and Infection Research, School of Biological Sciences, The University of Edinburgh, Ashworth Laboratories, The Kings Buildings, West Mains Road, Edinburgh EH9 3JT, UK
    Mol Biochem Parasitol 152:213-9. 2007
  15. ncbi request reprint Linkage Group Selection--a fast approach to the genetic analysis of malaria parasites
    Richard Carter
    University of Edinburgh, Institute of Immunology and Infection Research, School of Biological Sciences, West Mains Road, Edinburgh EH9 3JT, UK
    Int J Parasitol 37:285-93. 2007
    ..We have called the method Linkage Group Selection...
  16. ncbi request reprint Fitness of drug-resistant malaria parasites
    David Walliker
    Institute of Infection and Immunology Research, School of Biological Sciences, University of Edinburgh, Edinburgh EH9 3JT, UK
    Acta Trop 94:251-9. 2005
    ....
  17. pmc Genetics of mefloquine resistance in the rodent malaria parasite Plasmodium chabaudi
    Pedro V L Cravo
    Institute of Cell, Animal, and Population Biology, University of Edinburgh, Edinburgh EH9 3JT, United Kingdom
    Antimicrob Agents Chemother 47:709-18. 2003
    ..However, not all mefloquine-resistant progeny contained the duplicated gene, showing that at least one other gene was involved in resistance...
  18. ncbi request reprint Detection of mutations in the Plasmodium falciparum dihydrofolate reductase (dhfr) gene by dot-blot hybridization
    Abdel Muhsin A Abdel-Muhsin
    Institute of Cell, Animal and Population Biology, University of Edinburgh, Scotland, United Kingdom
    Am J Trop Med Hyg 67:24-7. 2002
    ..The method has been successfully extended for analysis of other point mutations in dhfr at residues 51 and 59, which are associated with a high level of pyrimethamine resistance...
  19. pmc Accurate and reliable high-throughput detection of copy number variation in the human genome
    Heike Fiegler
    The Wellcome Trust Sanger Institute, The Wellcome Trust Genome Campus, Hinxton, Cambridge CB10 1SA, United Kingdom
    Genome Res 16:1566-74. 2006
    ..Based on these studies, we developed a variance-based automatic copy number detection analysis process (CNVfinder) and have demonstrated its robustness by comparison with the SW-ARRAY method...