Stefan Howorka

Summary

Affiliation: University College London
Country: UK

Publications

  1. doi request reprint Microarrays and single molecules: an exciting combination
    Stefan Howorka
    Department of Chemistry, Institute for Structural and Molecular Biology, University College London, London WC1H 0AJ, UK
    Soft Matter 10:931-41. 2014
  2. doi request reprint DNA nanoarchitectonics: assembled DNA at interfaces
    Stefan Howorka
    Department of Chemistry, Institute of Structural Molecular Biology, University College London, London, England, United Kingdom
    Langmuir 29:7344-53. 2013
  3. doi request reprint Rationally engineering natural protein assemblies in nanobiotechnology
    Stefan Howorka
    Department of Chemistry, University College London, London WC1H 0AJ, UK
    Curr Opin Biotechnol 22:485-91. 2011
  4. doi request reprint Nanopore analytics: sensing of single molecules
    Stefan Howorka
    Department of Chemistry, Institute of Structural Molecular Biology, University College London, London, UK WC1H 0AJ
    Chem Soc Rev 38:2360-84. 2009
  5. pmc Diene-modified nucleotides for the Diels-Alder-mediated functional tagging of DNA
    Vinciane Borsenberger
    Department of Chemistry, Institute of Structural Molecular Biology, University College London, London WC1H 0AJ, UK
    Nucleic Acids Res 37:1477-85. 2009
  6. doi request reprint Identifying assembly-inhibiting and assembly-tolerant sites in the SbsB S-layer protein from Geobacillus stearothermophilus
    Helen Kinns
    Department of Chemistry, University College London, 20 Gordon Street, London WC1H 0AJ, UK
    J Mol Biol 395:742-53. 2010
  7. doi request reprint Chemically labeled nucleotides and oligonucleotides encode DNA for sensing with nanopores
    Vinciane Borsenberger
    Department of Chemistry, University College London, Christopher Ingold Building, 20 Gordon Street, London WC1H OAJ, UK
    J Am Chem Soc 131:7530-1. 2009
  8. doi request reprint Electrically sensing protease activity with nanopores
    Mikiembo Kukwikila
    Department of Chemistry, University College London, London WC1H 0AJ, UK
    J Phys Condens Matter 22:454103. 2010
  9. doi request reprint Chemical tags facilitate the sensing of individual DNA strands with nanopores
    Nick Mitchell
    Department of Chemistry, University College London, 20 Gordon Street, London WC1H OAJ, UK
    Angew Chem Int Ed Engl 47:5565-8. 2008
  10. ncbi request reprint Stochastic detection of motor protein-RNA complexes by single-channel current recording
    Yann Astier
    Department of Chemistry, Chemistry Research Laboratory, University of Oxford, UK
    Chemphyschem 8:2189-94. 2007

Collaborators

  • Hagan Bayley
  • Eugen Stulz
  • Daren J Caruana
  • Vinciane Borsenberger
  • Helen Kinns
  • Yann Astier
  • Nick Mitchell
  • Mikiembo Kukwikila
  • Jonathan R Burns
  • Hugh Martin
  • Eva Maria Egelseer
  • Uwe B Sleytr
  • Helga Badelt-Lichtblau
  • Denis E Kainov
  • Rethi Madathil
  • Roman Tuma

Detail Information

Publications16

  1. doi request reprint Microarrays and single molecules: an exciting combination
    Stefan Howorka
    Department of Chemistry, Institute for Structural and Molecular Biology, University College London, London WC1H 0AJ, UK
    Soft Matter 10:931-41. 2014
    ..The topic of this Highlight integrates several disciplines and is written for interested chemists, biophysicists and nanotechnologists. ..
  2. doi request reprint DNA nanoarchitectonics: assembled DNA at interfaces
    Stefan Howorka
    Department of Chemistry, Institute of Structural Molecular Biology, University College London, London, England, United Kingdom
    Langmuir 29:7344-53. 2013
    ..Possible future research developments are discussed at the end of the Perspective...
  3. doi request reprint Rationally engineering natural protein assemblies in nanobiotechnology
    Stefan Howorka
    Department of Chemistry, University College London, London WC1H 0AJ, UK
    Curr Opin Biotechnol 22:485-91. 2011
    ....
  4. doi request reprint Nanopore analytics: sensing of single molecules
    Stefan Howorka
    Department of Chemistry, Institute of Structural Molecular Biology, University College London, London, UK WC1H 0AJ
    Chem Soc Rev 38:2360-84. 2009
    ..Apart from being an analytical tool, nanopores have developed into a general platform technology to investigate the biophysics, physicochemistry, and chemistry of individual molecules (critical review, 310 references)...
  5. pmc Diene-modified nucleotides for the Diels-Alder-mediated functional tagging of DNA
    Vinciane Borsenberger
    Department of Chemistry, Institute of Structural Molecular Biology, University College London, London WC1H 0AJ, UK
    Nucleic Acids Res 37:1477-85. 2009
    ..Based on these advantages, diene- and cyclohexene-based nucleotide triphosphates are expected to find wider use in the area of nucleic acid chemistry...
  6. doi request reprint Identifying assembly-inhibiting and assembly-tolerant sites in the SbsB S-layer protein from Geobacillus stearothermophilus
    Helen Kinns
    Department of Chemistry, University College London, 20 Gordon Street, London WC1H 0AJ, UK
    J Mol Biol 395:742-53. 2010
    ..Our study marks an important step in the analysis of the S-layer structure. In addition, the approach of concerted insertion and cysteine mutagenesis can likely be applied for other supramolecular assemblies...
  7. doi request reprint Chemically labeled nucleotides and oligonucleotides encode DNA for sensing with nanopores
    Vinciane Borsenberger
    Department of Chemistry, University College London, Christopher Ingold Building, 20 Gordon Street, London WC1H OAJ, UK
    J Am Chem Soc 131:7530-1. 2009
    ..By being independent of pore engineering, the new approach can potentially enhance the sensing repertoire of durable solid-state nanopores for which alternative sensing strategies developed for protein pores are not easily accessible...
  8. doi request reprint Electrically sensing protease activity with nanopores
    Mikiembo Kukwikila
    Department of Chemistry, University College London, London WC1H 0AJ, UK
    J Phys Condens Matter 22:454103. 2010
    ..Owing to its simplicity, this approach to sense enzymatic activity may be applied to other proteases...
  9. doi request reprint Chemical tags facilitate the sensing of individual DNA strands with nanopores
    Nick Mitchell
    Department of Chemistry, University College London, 20 Gordon Street, London WC1H OAJ, UK
    Angew Chem Int Ed Engl 47:5565-8. 2008
  10. ncbi request reprint Stochastic detection of motor protein-RNA complexes by single-channel current recording
    Yann Astier
    Department of Chemistry, Chemistry Research Laboratory, University of Oxford, UK
    Chemphyschem 8:2189-94. 2007
    ..This study shows that single-channel current recordings can be used to monitor RNA-protein complex formation, thus opening up a new means to examine the motor activity of RNA- or DNA-processing enzymes...
  11. ncbi request reprint Nanoscale protein pores modified with PAMAM dendrimers
    Hugh Martin
    Department of Chemistry, University College London, London WC1H 0AJ, England, United Kingdom
    J Am Chem Soc 129:9640-9. 2007
    ..The alteration of pore properties with charged and hyperbranched dendrimers is a new approach and might be extended to inorganic nanopores with applications in sensing and separation technology...
  12. ncbi request reprint Self-assembled DNA nanopores that span lipid bilayers
    Jonathan R Burns
    Department of Chemistry, Institute of Structural Molecular Biology, University College London, London WC1H 0AJ, England, United Kingdom
    Nano Lett 13:2351-6. 2013
    ....
  13. ncbi request reprint Protein components for nanodevices
    Yann Astier
    Department of Chemistry, University of Oxford, OX1 3TA, England, UK
    Curr Opin Chem Biol 9:576-84. 2005
    ....
  14. doi request reprint Biosensors and biofuel cells with engineered proteins
    Daren J Caruana
    Department of Chemistry, University College London, London, WC1H 0AJ, UK
    Mol Biosyst 6:1548-56. 2010
    ..The focus of this review is to discuss the role of enzymes and highlight how protein engineering and immobilization can enhance device performance...
  15. doi request reprint The surface location of individual residues in a bacterial S-layer protein
    Helen Kinns
    Department of Chemistry, University College London, Christopher Ingold Building, 20 Gordon Street, London WC1H 0AJ, England, UK
    J Mol Biol 377:589-604. 2008
    ..Our approach of identifying the surface location of residues is relevant to other planar supramolecular protein assemblies...
  16. doi request reprint Synthesis and enzymatic incorporation of modified deoxyuridine triphosphates
    Vinciane Borsenberger
    University College London, Department of Chemistry, 20 Gordon Street, London, UKWC1H OAJ
    Org Biomol Chem 7:3826-35. 2009
    ..Both polymerases had difficulties incorporating 5-(3-amino-prop-1-ynyl)-2'-deoxyuridine triphosphate. A molecular model of the active site of the polymerase was used to rationalise why this nucleotide was not accepted as a substrate...