M D Houslay

Summary

Affiliation: University of Glasgow
Country: UK

Publications

  1. pmc The SH3 domain of Src tyrosyl protein kinase interacts with the N-terminal splice region of the PDE4A cAMP-specific phosphodiesterase RPDE-6 (RNPDE4A5)
    J C O'Connell
    Division of Biochemistry and Molecular Biology, I B L S, University of Glasgow, Scotland, U K
    Biochem J 318:255-61. 1996
  2. ncbi request reprint Spatial organisation of AKAP18 and PDE4 isoforms in renal collecting duct principal cells
    Theresa McSorley
    Forschungsinstitut fur Molekulare Pharmakologie, Campus Berlin Buch, Robert Rossle Strasse 10, D 13125 Berlin, Germany
    Eur J Cell Biol 85:673-8. 2006
  3. pmc Delineation of RAID1, the RACK1 interaction domain located within the unique N-terminal region of the cAMP-specific phosphodiesterase, PDE4D5
    Graeme B Bolger
    Veterans Affairs Medical Center, Huntsman Cancer Institute, Division of Oncology, Department of Medicine, University of Utah Health Sciences Center, Salt Lake City, UT 84148, USA
    BMC Biochem 3:24. 2002
  4. ncbi request reprint The long and short of vascular smooth muscle phosphodiesterase-4 as a putative therapeutic target
    Miles D Houslay
    Molecular Pharmacology Group, Division of Biochemistry and Molecular Biology, IBLS, Wolfson Building, University of Glasgow, Glasgow G12 8QQ, Scotland, UK
    Mol Pharmacol 68:563-7. 2005
  5. doi request reprint Arrestin times for developing antipsychotics and beta-blockers
    Miles D Houslay
    Neuroscience and Molecular Pharmacology, Wolfson and Davidson Buildings, University of Glasgow, Glasgow G12 8QQ, Scotland, UK
    Sci Signal 2:pe22. 2009
  6. ncbi request reprint Keynote review: phosphodiesterase-4 as a therapeutic target
    Miles D Houslay
    Division of Biochemistry and Molecular Biology, IBLS, Wolfson Link Building, University of Glasgow, University Avenue, Glasgow, G12 8QQ, Scotland, UK
    Drug Discov Today 10:1503-19. 2005
  7. ncbi request reprint The role of ERK2 docking and phosphorylation of PDE4 cAMP phosphodiesterase isoforms in mediating cross-talk between the cAMP and ERK signalling pathways
    M D Houslay
    Molecular Pharmacology Group, Division of Biochemistry and Molecular Biology, Wolfson Building, Institute of Biomedical and Life Sciences, University of Glasgow, Glasgow G12 8QQ, Scotland, U K
    Biochem Soc Trans 31:1186-90. 2003
  8. ncbi request reprint Beta-arrestin-recruited phosphodiesterase-4 desensitizes the AKAP79/PKA-mediated switching of beta2-adrenoceptor signalling to activation of ERK
    M D Houslay
    Division of Biochemistry and Molecular Biology, IBLS, Wolfson Building, University of Glasgow, Glasgow G12 8QQ, Scotland, UK
    Biochem Soc Trans 33:1333-6. 2005
  9. ncbi request reprint Tailoring cAMP-signalling responses through isoform multiplicity
    M D Houslay
    Division of Biochemistry and Molecular Biology, University of Glasgow, Scotland, UK
    Trends Biochem Sci 22:217-24. 1997
  10. ncbi request reprint A RSK(y) relationship with promiscuous PKA
    Miles D Houslay
    Molecular Pharmacology Group, Division of Biochemistry and Molecular Biology, Institute of Biomedical and Life Sciences, Wolfson Building, University of Glasgow, Glasgow G12 8QQ, Scotland, UK
    Sci STKE 2006:pe32. 2006

Detail Information

Publications106 found, 100 shown here

  1. pmc The SH3 domain of Src tyrosyl protein kinase interacts with the N-terminal splice region of the PDE4A cAMP-specific phosphodiesterase RPDE-6 (RNPDE4A5)
    J C O'Connell
    Division of Biochemistry and Molecular Biology, I B L S, University of Glasgow, Scotland, U K
    Biochem J 318:255-61. 1996
    ....
  2. ncbi request reprint Spatial organisation of AKAP18 and PDE4 isoforms in renal collecting duct principal cells
    Theresa McSorley
    Forschungsinstitut fur Molekulare Pharmakologie, Campus Berlin Buch, Robert Rossle Strasse 10, D 13125 Berlin, Germany
    Eur J Cell Biol 85:673-8. 2006
    ..AKAP-anchored PKA has been shown to be involved in AQP2 shuttling. Here, AKAP18 isoforms and members of the PDE4 family of PDEs are shown to be differentially localised in renal principal cells...
  3. pmc Delineation of RAID1, the RACK1 interaction domain located within the unique N-terminal region of the cAMP-specific phosphodiesterase, PDE4D5
    Graeme B Bolger
    Veterans Affairs Medical Center, Huntsman Cancer Institute, Division of Oncology, Department of Medicine, University of Utah Health Sciences Center, Salt Lake City, UT 84148, USA
    BMC Biochem 3:24. 2002
    ..Two-hybrid analysis of truncation and mutant constructs of the unique N-terminal region of the cAMP-specific phosphodiesterase, PDE4D5 were used to define a domain conferring interaction with the signaling scaffold protein, RACK1...
  4. ncbi request reprint The long and short of vascular smooth muscle phosphodiesterase-4 as a putative therapeutic target
    Miles D Houslay
    Molecular Pharmacology Group, Division of Biochemistry and Molecular Biology, IBLS, Wolfson Building, University of Glasgow, Glasgow G12 8QQ, Scotland, UK
    Mol Pharmacol 68:563-7. 2005
    ....
  5. doi request reprint Arrestin times for developing antipsychotics and beta-blockers
    Miles D Houslay
    Neuroscience and Molecular Pharmacology, Wolfson and Davidson Buildings, University of Glasgow, Glasgow G12 8QQ, Scotland, UK
    Sci Signal 2:pe22. 2009
    ....
  6. ncbi request reprint Keynote review: phosphodiesterase-4 as a therapeutic target
    Miles D Houslay
    Division of Biochemistry and Molecular Biology, IBLS, Wolfson Link Building, University of Glasgow, University Avenue, Glasgow, G12 8QQ, Scotland, UK
    Drug Discov Today 10:1503-19. 2005
    ..Here, we delineate the range of PDE4 isoforms, their role in signaling, their structural biology and related preclinical and clinical pharmacology...
  7. ncbi request reprint The role of ERK2 docking and phosphorylation of PDE4 cAMP phosphodiesterase isoforms in mediating cross-talk between the cAMP and ERK signalling pathways
    M D Houslay
    Molecular Pharmacology Group, Division of Biochemistry and Molecular Biology, Wolfson Building, Institute of Biomedical and Life Sciences, University of Glasgow, Glasgow G12 8QQ, Scotland, U K
    Biochem Soc Trans 31:1186-90. 2003
    ..Recruited PDE4 thus desensitizes the ability of the beta(2)-adrenoceptor to activate ERK via G(i)...
  8. ncbi request reprint Beta-arrestin-recruited phosphodiesterase-4 desensitizes the AKAP79/PKA-mediated switching of beta2-adrenoceptor signalling to activation of ERK
    M D Houslay
    Division of Biochemistry and Molecular Biology, IBLS, Wolfson Building, University of Glasgow, Glasgow G12 8QQ, Scotland, UK
    Biochem Soc Trans 33:1333-6. 2005
    ....
  9. ncbi request reprint Tailoring cAMP-signalling responses through isoform multiplicity
    M D Houslay
    Division of Biochemistry and Molecular Biology, University of Glasgow, Scotland, UK
    Trends Biochem Sci 22:217-24. 1997
    ..The ability to breach the PKA activation threshold can depend upon either or both the activation of adenylate cyclase and inhibition of specific PDE isoforms...
  10. ncbi request reprint A RSK(y) relationship with promiscuous PKA
    Miles D Houslay
    Molecular Pharmacology Group, Division of Biochemistry and Molecular Biology, Institute of Biomedical and Life Sciences, Wolfson Building, University of Glasgow, Glasgow G12 8QQ, Scotland, UK
    Sci STKE 2006:pe32. 2006
    ....
  11. ncbi request reprint cAMP-Specific phosphodiesterase-4 enzymes in the cardiovascular system: a molecular toolbox for generating compartmentalized cAMP signaling
    Miles D Houslay
    Molecular Pharmacology Group, Division of Biochemistry and Molecular Biology, Institute of Biomedical and Life Sciences, University of Glasgow, Glasgow G12 8QQ, Scotland, UK
    Circ Res 100:950-66. 2007
    ....
  12. pmc PDE4 cAMP phosphodiesterases: modular enzymes that orchestrate signalling cross-talk, desensitization and compartmentalization
    Miles D Houslay
    Molecular Pharmacology Group, Division of Biochemistry and Molecular Biology, Davidson Building, Institute of Biomedical and Life Sciences, University of Glasgow, Glasgow G12 8QQ, Scotland, UK
    Biochem J 370:1-18. 2003
    ..PDE4 enzymes stand at a crossroads that allows them to integrate various signalling pathways with that of cAMP in spatially distinct compartments...
  13. doi request reprint Underpinning compartmentalised cAMP signalling through targeted cAMP breakdown
    Miles D Houslay
    Neuroscience and Molecular Pharmacology, FBLS, University of Glasgow, University Avenue, Glasgow, G12 8QQ, Scotland, UK
    Trends Biochem Sci 35:91-100. 2010
    ....
  14. doi request reprint p62 (SQSTM1) forms part of a novel, reversible aggregate containing a specific conformer of the cAMP degrading phosphodiesterase, PDE4A4
    Miles D Houslay
    Molecular Pharmacology Group, Wolfson Link and Davidson Buildings, Institute of Neuroscience and Psychology, College of Medical, Veterinary and Life Sciences, University of Glasgow, Glasgow, Scotand, UK
    Autophagy 6:1198-200. 2010
    ..This results in PDE4A4 becoming sequestered away from signaling proteins that normally sequester it, providing a means of reprogramming compartmentalized cAMP signaling in cells...
  15. pmc Disrupting specific PDZ domain-mediated interactions for therapeutic benefit
    Miles D Houslay
    Neuroscience and Molecular Pharmacology, Faculty of Biomedical and Life Sciences, University of Glasgow, Glasgow, UK
    Br J Pharmacol 158:483-5. 2009
    ....
  16. ncbi request reprint Molecular cloning, genomic positioning, promoter identification, and characterization of the novel cyclic amp-specific phosphodiesterase PDE4A10
    G Rena
    Molecular Pharmacology Group, Division of Biochemistry and Molecular Biology, Institute of Biomedical and Life Sciences, University of Glasgow, Glasgow, Scotland, United Kingdom
    Mol Pharmacol 59:996-1011. 2001
    ..We suggest that the unique N-terminal regions of PDE4A isoforms confer distinct properties upon them...
  17. ncbi request reprint Association with the SRC family tyrosyl kinase LYN triggers a conformational change in the catalytic region of human cAMP-specific phosphodiesterase HSPDE4A4B. Consequences for rolipram inhibition
    I McPhee
    Division of Biochemistry and Molecular Biology, IBLS, Davidson Building, University of Glasgow, Glasgow G12 8QQ, Scotland, United Kingdom
    J Biol Chem 274:11796-810. 1999
    ..Interaction between pde46 and SRC family tyrosyl kinases highlights a potentially novel regulatory system and point of signaling system cross-talk...
  18. pmc cAMP-specific phosphodiesterase HSPDE4D3 mutants which mimic activation and changes in rolipram inhibition triggered by protein kinase A phosphorylation of Ser-54: generation of a molecular model
    R Hoffmann
    Molecular Pharmacology Group, Division of Biochemistry and Molecular Biology, Davidson and Wolfson Buildings, IBLS, University of Glasgow, Glasgow G12 8QQ, Scotland, U K
    Biochem J 333:139-49. 1998
    ..The increase in susceptibility to inhibition by rolipram upon PKA-mediated phosphorylation is suggested to involve the disruption of a hydrogen-bond involving the side-chain hydroxy group of Ser-54...
  19. ncbi request reprint The unique N-terminal domain of the cAMP phosphodiesterase PDE4D4 allows for interaction with specific SH3 domains
    M B Beard
    Molecular Pharmacology Group, Division of Biochemistry and Molecular Biology, University of Glasgow, UK
    FEBS Lett 460:173-7. 1999
    ..PDE4D4 did not bind to WW domains. We suggest that an important function of the unique N-terminal region of PDE4D4 may be to allow for association with certain SH3 domain-containing proteins...
  20. ncbi request reprint ERK2 mitogen-activated protein kinase binding, phosphorylation, and regulation of the PDE4D cAMP-specific phosphodiesterases. The involvement of COOH-terminal docking sites and NH2-terminal UCR regions
    S J Mackenzie
    Molecular Pharmacology Group, Division of Biochemistry and Molecular Biology, Davidson Bldg, IBLS, University of Glasgow, Glasgow G12 8QQ, Scotland, United Kingdom
    J Biol Chem 275:16609-17. 2000
    ..The PDE4D gene thus encodes a series of isoenzymes that are either inhibited or activated by ERK2 phosphorylation and thereby offers the potential for ERK2 activation either to increase or decrease cAMP levels in cellular compartments...
  21. pmc The MAP kinase ERK2 inhibits the cyclic AMP-specific phosphodiesterase HSPDE4D3 by phosphorylating it at Ser579
    R Hoffmann
    Molecular Pharmacology Group, Division of Biochemistry and Molecular Biology, Davidson and Wolfson Buildings, IBLS, University of Glasgow, Glasgow G12 8QQ, UK
    EMBO J 18:893-903. 1999
    ..We identify a novel means of cross-talk between the cAMP and ERK signalling pathways whereby cell stimuli that lead to ERK2 activation may modulate cAMP signalling...
  22. pmc Molecular cloning and transient expression in COS7 cells of a novel human PDE4B cAMP-specific phosphodiesterase, HSPDE4B3
    E Huston
    Division of Biochemistry and Molecular Biology, Wolfson Building, University of Glasgow, Glasgow G12 8QQ, Scotland, U K
    Biochem J 328:549-58. 1997
    ..It is suggested that these alternatively spliced regions determine changes in the maximal catalytic activity of the isoforms, their susceptibility to inhibition by rolipram and mode of interaction with particulate fractions...
  23. pmc Growth hormone decreases the response to anti-lipolytic agonists and decreases the levels of Gi2 in rat adipocytes
    R Doris
    Hannah Research Institute, Ayr, Scotland, U K
    Biochem J 297:41-5. 1994
    ..It is concluded that GH down-regulates the amount of Gi2 alpha-subunit in adipocyte membranes, resulting in a decrease in the sensitivity of the cells to anti-lipolytic agonists...
  24. ncbi request reprint The RACK1 signaling scaffold protein selectively interacts with the cAMP-specific phosphodiesterase PDE4D5 isoform
    S J Yarwood
    Molecular Pharmacology Group, Division of Biochemistry and Molecular Biology, Institute of Biology and Life Sciences, University of Glasgow, Davidson Building, Glasgow G12 8QQ, Scotland, United Kingdom
    J Biol Chem 274:14909-17. 1999
    ..We suggest that RACK1 may act as a scaffold protein to recruit PDE4D5 and other proteins into a signaling complex...
  25. ncbi request reprint The human cyclic AMP-specific phosphodiesterase PDE-46 (HSPDE4A4B) expressed in transfected COS7 cells occurs as both particulate and cytosolic species that exhibit distinct kinetics of inhibition by the antidepressant rolipram
    E Huston
    Molecular Pharmacology Group, Division of Biochemistry and Molecular Biology, IBLS, Wolfson Link Building, University of Glasgow, Glasgow G12 8QQ, Scotland, United Kingdom
    J Biol Chem 271:31334-44. 1996
    ..6 microM) but as a partial competitive inhibitor of the particulate enzyme (Ki = 0.037 microM; Ki' = 2.3 microM). Particulate PDE-46 thus showed a approximately 60-fold higher affinity for rolipram than cytosolic PDE-46...
  26. pmc Identification and characterization of the human homologue of the short PDE4A cAMP-specific phosphodiesterase RD1 (PDE4A1) by analysis of the human HSPDE4A gene locus located at chromosome 19p13.2
    M Sullivan
    Molecular Pharmacology Group, Division of Biochemistry and Molecular Biology, IBLS, Davidson and Wolfson Buildings, University of Glasgow, Glasgow G12 8QQ, Scotland, UK
    Biochem J 333:693-703. 1998
    ....
  27. ncbi request reprint Regulation of the GTP-binding protein-based antilipolytic system of sheep adipocytes by growth hormone
    R A Doris
    Hannah Research Institute, Ayr, Scotland, UK
    J Endocrinol 158:295-303. 1998
    ..Thus the attenuation of the inhibition of lipolysis by PIA by chronic exposure of adipocytes to GH appears to be due to an impairment in the interaction between adenylate cyclase and the alpha subunit of one or more isoforms of Gi...
  28. ncbi request reprint Genomic organisation of the human cyclic AMP-specific phosphodiesterase PDE4C gene and its chromosomal localisation to 19p13.1, between RAB3A and JUND
    M Sullivan
    Division of Biochemistry and Molecular Biology, Institute of Biomedical and Life Sciences, University of Glasgow, UK
    Cell Signal 11:735-42. 1999
    ..7 kb separate PDE4C and RAB3A. The three genes share the same orientation of transcription and are arranged in the order cen- 5'- JUND-PDE4C-RAB3A-3'-tel...
  29. ncbi request reprint cAMP-specific phosphodiesterase-4D5 (PDE4D5) provides a paradigm for understanding the unique non-redundant roles that PDE4 isoforms play in shaping compartmentalized cAMP cell signalling
    M J Lynch
    Division of Biochemistry and Molecular Biology, IBLS Institute of Biomedical and Life Sciences, Wolfson Building, University of Glasgow, Glasgow G12 8QQ, Scotland, U K
    Biochem Soc Trans 35:938-41. 2007
    ....
  30. doi request reprint Disruption of the cyclic AMP phosphodiesterase-4 (PDE4)-HSP20 complex attenuates the β-agonist induced hypertrophic response in cardiac myocytes
    Y Y Sin
    Molecular Pharmacology Group, Wolfson Link and Davidson Buildings, Institute for Psychology and Neurosciences, College of Medical, Veterinary and Life Sciences, University of Glasgow, Glasgow G12 8QQ, Scotland, UK
    J Mol Cell Cardiol 50:872-83. 2011
    ....
  31. ncbi request reprint Phorbol 12-myristate 13-acetate triggers the protein kinase A-mediated phosphorylation and activation of the PDE4D5 cAMP phosphodiesterase in human aortic smooth muscle cells through a route involving extracellular signal regulated kinase (ERK)
    G Baillie
    Molecular Pharmacology Group, Division of Biochemistry and Molecular Biology, Institute of Biomedical and Life Sciences, University of Glasgow, Glasgow, Scotland, United Kingdom
    Mol Pharmacol 60:1100-11. 2001
    ..This involves PGE(2) generation, which causes activation of adenylyl cyclase, allowing PKA to elicit net activation of PDE4D5 by phosphorylation at Ser126...
  32. pmc β-Arrestin 1 inhibits the GTPase-activating protein function of ARHGAP21, promoting activation of RhoA following angiotensin II type 1A receptor stimulation
    D F Anthony
    Neuroscience and Molecular Pharmacology, Division of Integrative Biology, IBLS, Wolfson Building, University of Glasgow, Glasgow G12 8QQ, Scotland, United Kingdom
    Mol Cell Biol 31:1066-75. 2011
    ....
  33. pmc A simple method for sequencing small DNAs by introducing precise overlapping ends into restriction digestion fragments
    G Rena
    Division of Biochemistry and Molecular Biology, Davidson Building, IBLS, University of Glasgow, Glasgow G12 8QQ, UK
    Nucleic Acids Res 26:3867-8. 1998
    ..This is a simple approach to ordering digestion fragments without necessarily performing restriction mapping. It is envisaged that this technique will be useful for sequencing cDNAs and small genomic fragments...
  34. ncbi request reprint In resting COS1 cells a dominant negative approach shows that specific, anchored PDE4 cAMP phosphodiesterase isoforms gate the activation, by basal cyclic AMP production, of AKAP-tethered protein kinase A type II located in the centrosomal region
    Angela McCahill
    Molecular Pharmacology Group, Division of Biochemistry and Molecular Biology, IBLS, Wolfson Link Building, University of Glasgow, Glasgow G12 8QQ, Scotland, UK
    Cell Signal 17:1158-73. 2005
    ..We propose that a novel role for PDE4D3 and PDE4C2 is to gate the activation of AKAP450-tethered PKA type-II localised in the perinuclear region under conditions of basal cAMP generation in resting cells...
  35. pmc beta-Arrestin-mediated PDE4 cAMP phosphodiesterase recruitment regulates beta-adrenoceptor switching from Gs to Gi
    George S Baillie
    Molecular Pharmacology Group, Division of Biochemistry and Molecular Biology, Institute for Biomedical and Life Sciences, University of Glasgow, Glasgow G12 8QQ, United Kingdom
    Proc Natl Acad Sci U S A 100:940-5. 2003
    ..Thus, receptor-stimulated beta-arrestin-mediated recruitment of PDE4 plays a central role in the regulation of G protein switching by the beta(2)AR in a physiological system, the cardiac myocyte...
  36. pmc Long PDE4 cAMP specific phosphodiesterases are activated by protein kinase A-mediated phosphorylation of a single serine residue in Upstream Conserved Region 1 (UCR1)
    Simon J Mackenzie
    Molecular Pharmacology Group, Division of Biochemistry and Molecular Biology, Davidson and Wolfson Buildings, Institute of Biomedical and Life Sciences, University of Glasgow, Glasgow G12 8QQ, Scotland, UK
    Br J Pharmacol 136:421-33. 2002
    ..This leads to an increase in their activity and may thus contribute to cellular desensitization processes in cells where these isoforms are selectively expressed...
  37. ncbi request reprint cAMP phosphodiesterase-4A1 (PDE4A1) has provided the paradigm for the intracellular targeting of phosphodiesterases, a process that underpins compartmentalized cAMP signalling
    E Huston
    Molecular Pharmacology Group, Division of Biochemistry and Molecular Biology, Institute of Biomedical and Life Sciences, University of Glasgow, Glasgow G12 8QQ, Scotland, UK
    Biochem Soc Trans 34:504-9. 2006
    ..The irreversible, Ca(2+)-dependent insertion of the N-terminal region of PDE4A1 into membranes provides 'long-term' memory of cell activation...
  38. pmc The role of the PDE4D cAMP phosphodiesterase in the regulation of glucagon-like peptide-1 release
    W K Ong
    Strathclyde Institute of Pharmacy, Cell Biology Group, University of Strathclyde, Glasgow, UK
    Br J Pharmacol 157:633-44. 2009
    ..As cAMP is hydrolysed by cAMP phosphodiesterases (PDEs), we determined the role of PDEs and particularly PDE4 in regulating GLP-1 release...
  39. ncbi request reprint Interaction of caspase-3 with the cyclic GMP binding cyclic GMP specific phosphodiesterase (PDE5a1)
    Mhairi J Frame
    Department of Physiology and Pharmacology, Strathclyde Institute for Biomedical Sciences, University of Strathclyde, 27 Taylor Street, Glasgow G4 0NR, Scotland, UK
    Eur J Biochem 270:962-70. 2003
    ..From this, we can predict that a caspase-3-mediated cleavage of the [778]DQGD[781] motif would result in removal of the C-terminal tail containing Q807 and F810, which are potentially important amino acids required for substrate binding...
  40. doi request reprint Ndel1 alters its conformation by sequestering cAMP-specific phosphodiesterase-4D3 (PDE4D3) in a manner that is dynamically regulated through Protein Kinase A (PKA)
    Daniel M Collins
    Neuroscience and Molecular Pharmacology, Faculty of Biomedical and Life Sciences, Wolfson Link and Davidson Buildings, University of Glasgow, University Avenue, Glasgow, G12 8QQ, Scotland, UK
    Cell Signal 20:2356-69. 2008
    ..We propose that Ser13 may act as a redistribution trigger in PDE4D3, allowing it to dynamically re-shape cAMP gradients in distinct intracellular locales upon its phosphorylation by PKA...
  41. ncbi request reprint Identification and characterization of PDE4A11, a novel, widely expressed long isoform encoded by the human PDE4A cAMP phosphodiesterase gene
    Derek A Wallace
    Molecular Pharmacology Group, Division of Biochemistry and Molecular Biology, Institute of Biomedical and Life Sciences, Wolfson Building, University Avenue, University of Glasgow, Glasgow G12 8QQ, Scotland, UK
    Mol Pharmacol 67:1920-34. 2005
    ....
  42. ncbi request reprint Isoform-selective susceptibility of DISC1/phosphodiesterase-4 complexes to dissociation by elevated intracellular cAMP levels
    Hannah Murdoch
    Molecular Pharmacology Group, Division of Biochemistry and Molecular Biology, Institute of Biomedical and Life Sciences, University of Glasgow, Glasgow G12 8QQ, United Kingdom
    J Neurosci 27:9513-24. 2007
    ..Thus, genetic variation in DISC1 and PDE4 that influence either isoform expression or docking site functioning may directly affect psychopathology...
  43. pmc Receptor-mediated stimulation of lipid signalling pathways in CHO cells elicits the rapid transient induction of the PDE1B isoform of Ca2+/calmodulin-stimulated cAMP phosphodiesterase
    S Spence
    Division of Biochemistry and Molecular Biology, University of Glasgow, Scotland, U K
    Biochem J 321:157-63. 1997
    ....
  44. pmc A novel role for a Drosophila homologue of cGMP-specific phosphodiesterase in the active transport of cGMP
    Jonathan P Day
    Institute of Biomedical and Life Sciences, Division of Molecular Genetics, University of Glasgow, Glasgow G11 6NU, UK
    Biochem J 393:481-8. 2006
    ..We provide the first demonstration of a novel role for a cG-PDE in modulating cGMP transport and efflux...
  45. pmc EPAC and PKA allow cAMP dual control over DNA-PK nuclear translocation
    Elaine Huston
    Neuroscience and Molecular Pharmacology, Faculty of Biomedical and Life Sciences, University of Glasgow, Glasgow G12 8QQ, Scotland, UK
    Proc Natl Acad Sci U S A 105:12791-6. 2008
    ..Through this signaling system EPAC activation can thereby impact on the Ser-473 phosphorylation status of PKB/Akt and the repair of etoposide-induced DSBs...
  46. ncbi request reprint Hypoxia-induced remodelling of PDE4 isoform expression and cAMP handling in human pulmonary artery smooth muscle cells
    Jennifer Millen
    Division of Neuroscience and Biomedical Systems, Institute of Biomedical and Life Sciences, University of Glasgow, Glasgow G12 8QQ, Scotland, UK
    Eur J Cell Biol 85:679-91. 2006
    ..The hypoxia-induced increase in cAMP may represent a compensatory protective mechanism against hypoxia-induced mitogens such as endothelin-1 and serotonin...
  47. ncbi request reprint RNA silencing identifies PDE4D5 as the functionally relevant cAMP phosphodiesterase interacting with beta arrestin to control the protein kinase A/AKAP79-mediated switching of the beta2-adrenergic receptor to activation of ERK in HEK293B2 cells
    Martin J Lynch
    Division of Biochemistry and Molecular Biology, Institute of Biomedical and Life Sciences, Wolfson Building, University of Glasgow, Glasgow G12 8QQ, Scotland, United Kingdom
    J Biol Chem 280:33178-89. 2005
    ..The ability to observe a cellular phenotype upon PDE4D5 knockdown demonstrates that other PDE4 isoforms, expressed at endogenous levels, are unable to afford rescue in HEK293B2 cells...
  48. ncbi request reprint The dg2 (for) gene confers a renal phenotype in Drosophila by modulation of cGMP-specific phosphodiesterase
    Matthew R MacPherson
    Institute of Biomedical and Life Sciences, Division of Molecular Genetics, University of Glasgow, Glasgow G11 6NU, UK
    J Exp Biol 207:2769-76. 2004
    ....
  49. ncbi request reprint PDE4-regulated cAMP degradation controls the assembly of integrin-dependent actin adhesion structures and REF52 cell migration
    Yvonne M Fleming
    Institute of Biological and Life Sciences, Davidson and Wolfson Buildings, University of Glasgow, Glasgow G12 8QQ, UK
    J Cell Sci 117:2377-88. 2004
    ..Our data demonstrate that PDE4 activity is a key modulator of integrin-induced actin assembly at the cell periphery which, in turn, controls cell migration...
  50. ncbi request reprint Phosphodiesterase-4 gates the ability of protein kinase A to phosphorylate G-protein receptor kinase-2 and influence its translocation
    M D Houslay
    Molecular Pharmacology Group, Division of Biochemistry and Molecular Biology, IBLS, Wolfson Link Building, University of Glasgow, University Avenue, Glasgow G12 8QQ, Scotland, UK
    Biochem Soc Trans 34:474-5. 2006
    ..We propose that PDE4 activity protects GRK2 from inappropriate phosphorylation by PKA in resting cells that might have occurred through fluctuations in basal cAMP levels. Thus PDE4 gates the action of PKA to phosphorylate GRK2...
  51. doi request reprint In cardiac myocytes, cAMP elevation triggers the down-regulation of transcripts and promoter activity for cyclic AMP phosphodiesterase-4A10 (PDE4A10)
    Angela McCahill
    Neuroscience and Molecular Pharmacology, Wolfson Link and Davidson Buildings, Faculty of Biomedical and Life Sciences, University of Glasgow, University Avenue, Glasgow G12 8QQ, Scotland, UK
    Cell Signal 20:2071-83. 2008
    ....
  52. doi request reprint Mutations of beta-arrestin 2 that limit self-association also interfere with interactions with the beta2-adrenoceptor and the ERK1/2 MAPKs: implications for beta2-adrenoceptor signalling via the ERK1/2 MAPKs
    Tian Rui Xu
    Sir Henry Welcome Functional Genomics Facility, Division of Biochemistry and Molecular Biology, Institute of Biomedical and Life Sciences, University of Glasgow, Glasgow G12 8QQ, Scotland, UK
    Biochem J 413:51-60. 2008
    ..Regulation of beta-arrestin 2 self-association may therefore control beta-arrestin 2-mediated beta2-adrenoceptor-ERK1/2 MAPK signalling...
  53. pmc Phosphodiesterase-4 influences the PKA phosphorylation status and membrane translocation of G-protein receptor kinase 2 (GRK2) in HEK-293beta2 cells and cardiac myocytes
    Xiang Li
    Molecular Pharmacology Group, Division of Biochemistry and Molecular Biology, IBLS, Wolfson Link Building, University of Glasgow, University Avenue, Glasgow G12 8QQ, Scotland, UK
    Biochem J 394:427-35. 2006
    ....
  54. pmc Mdm2 directs the ubiquitination of beta-arrestin-sequestered cAMP phosphodiesterase-4D5
    Xiang Li
    Neuroscience and Molecular Pharmacology, Wolfson and Davidson Buildings, Faculty of Biomedical and Life Sciences, University of Glasgow, Glasgow G12 8QQ, Scotland, United Kingdom
    J Biol Chem 284:16170-82. 2009
    ....
  55. ncbi request reprint Human PDE4A8, a novel brain-expressed PDE4 cAMP-specific phosphodiesterase that has undergone rapid evolutionary change
    Kirsty F MacKenzie
    Molecular Pharmacology Group, Division of Biochemistry and Molecular Biology, Institute of Biomedical and Life Sciences, University of Glasgow, Glasgow G12 8QQ, Scotland, UK
    Biochem J 411:361-9. 2008
    ..The unique tissue distribution of PDE4A8, combined with the evolutionary divergence of its N-terminus, suggest that this isoform may have a specific function in regulating cAMP levels in human skeletal muscle and brain...
  56. pmc Cyclic nucleotide phosphodiesterases in Drosophila melanogaster
    Jonathan P Day
    Division of Molecular Genetics, Institute of Biomedical and Life Sciences, University of Glasgow, Glasgow G11 6NU, UK
    Biochem J 388:333-42. 2005
    ..12+/-0.06 microM for PDE11). We provide the first characterization of a cGMP-specific PDE and two dual-specificity PDEs in Drosophila, and show a high degree of similarity in structure and function between human and Drosophila PDEs...
  57. ncbi request reprint Compartmentalisation of phosphodiesterases and protein kinase A: opposites attract
    George S Baillie
    Molecular Pharmacology Group, Division of Biochemistry and Molecular Biology, IBLS, Wolfson Building, University of Glasgow, Glasgow G12 8QQ, Scotland, UK
    FEBS Lett 579:3264-70. 2005
    ..Compartmentalisation of PDEs not only provides an elegant means to control PKA activation by monitoring the local cAMP flux, but also serves to concentrate and segregate the action of these important regulatory enzymes...
  58. ncbi request reprint Investigation of extracellular signal-regulated kinase 2 mitogen-activated protein kinase phosphorylation and regulation of activity of PDE4 cyclic adenosine monophosphate-specific phosphodiesterases
    Elaine V Hill
    Molecular Pharmacology Group, Division of Biochemistry and Molecular Biology, Lund University, Biomedical Center, Sweden
    Methods Mol Biol 307:225-37. 2005
    ..We detail some of the methods that have been crucial in elucidating these important discoveries that represent a novel point of cross talk between the cAMP signaling system and the ERK mitogen-activated protein kinase cascade...
  59. ncbi request reprint Arrestin times for compartmentalised cAMP signalling and phosphodiesterase-4 enzymes
    George S Baillie
    Molecular Pharmacology Group, Division of Biochemistry and Molecular Biology, Institute of Biomedical and Life Sciences, Wolfson Building, University Avenue, University of Glasgow, Glasgow, G12 8QQ, Scotland, UK
    Curr Opin Cell Biol 17:129-34. 2005
    ..The co-receptor CD28 enhances signalling through the T-cell receptor by recruiting a PDE4/beta-arrestin complex, which then attenuates PKA phosphorylation of Csk...
  60. pmc Stimulation of p70S6 kinase via a growth hormone-controlled phosphatidylinositol 3-kinase pathway leads to the activation of a PDE4A cyclic AMP-specific phosphodiesterase in 3T3-F442A preadipocytes
    S J Mackenzie
    Division of Biochemistry, Davidson and Wolfson Buildings, University of Glasgow, Glasgow G12 8QQ, Scotland, United Kingdom
    Proc Natl Acad Sci U S A 95:3549-54. 1998
    ..It is suggested that activation of PDE4A5 by growth hormone serves as a brake on the differentiation processes...
  61. pmc Hormonal regulation of Gi2 alpha-subunit phosphorylation in intact hepatocytes
    M Bushfield
    Department of Biochemistry, University of Glasgow, Scotland, U K
    Biochem J 268:449-57. 1990
    ..We suggest that there are two possible sites for the phosphorylation of alpha-Gi2; one for C-kinase and the other for an unidentified kinase whose action is triggered by A-kinase activation...
  62. pmc Mapping binding sites for the PDE4D5 cAMP-specific phosphodiesterase to the N- and C-domains of beta-arrestin using spot-immobilized peptide arrays
    George S Baillie
    Molecular Pharmacology Group, Division of Biochemistry and Molecular Biology, Institute of Biomedical and Life Sciences, University of Glasgow, Glasgow G12 8QQ, Scotland, UK
    Biochem J 404:71-80. 2007
    ..These data show that the interaction of PDE4D5 with both the N- and C-domains of beta-arrestin 2 are essential for beta2-AR regulation...
  63. ncbi request reprint PDE4B5, a novel, super-short, brain-specific cAMP phosphodiesterase-4 variant whose isoform-specifying N-terminal region is identical to that of cAMP phosphodiesterase-4D6 (PDE4D6)
    York Fong Cheung
    Molecular Pharmacology Group, Division of Biochemistry and Molecular Biology, Institute of Biomedical and Life Sciences, University of Glasgow, Glasgow, Scotland, United Kingdom
    J Pharmacol Exp Ther 322:600-9. 2007
    ..Understanding the distinct tissue specificity of PDE4 isoforms will be important for understanding phosphodiesterase biology and opportunities for therapeutic intervention...
  64. ncbi request reprint Oxidative stress employs phosphatidyl inositol 3-kinase and ERK signalling pathways to activate cAMP phosphodiesterase-4D3 (PDE4D3) through multi-site phosphorylation at Ser239 and Ser579
    Elaine V Hill
    Molecular Pharmacology Group, Division of Biochemistry and Molecular Biology, Institute of Biomedical and Life Sciences, University of Glasgow, Glasgow G12 8QQ, Scotland, UK
    Cell Signal 18:2056-69. 2006
    ..We identify a novel process through which reactive oxygen species activate long PDE4 isoforms so as to reduce cAMP levels and thereby promote inflammatory responses...
  65. doi request reprint Regulation of a Drosophila melanogaster cGMP-specific phosphodiesterase by prenylation and interaction with a prenyl-binding protein
    Jonathan P Day
    Institute of Biomedical and Life Sciences, Division of Molecular Genetics, University of Glasgow, Glasgow G11 6NU, Scotland, UK
    Biochem J 414:363-74. 2008
    ..This suggests that, in addition to prenylation and interaction with DmPrBP/delta, further functional membrane-targeting signals exist within DmPDE5/6...
  66. doi request reprint Selective SUMO modification of cAMP-specific phosphodiesterase-4D5 (PDE4D5) regulates the functional consequences of phosphorylation by PKA and ERK
    Xiang Li
    Neuroscience and Molecular Pharmacology, Division of Integrative Biology, IBLS, Wolfson Building, University of Glasgow, Glasgow G12 8QQ, Scotland, UK
    Biochem J 428:55-65. 2010
    ..These results highlight a new means whereby cells might achieve the selective regulation of the activity of cAMP-specific PDE4 enyzmes...
  67. pmc Molecular cloning and subcellular distribution of the novel PDE4B4 cAMP-specific phosphodiesterase isoform
    Malcolm Shepherd
    Molecular Pharmacology Group, Division of Biochemistry and Molecular Biology, Institute of Biology and Life Sciences, University of Glasgow, Davidson Building, Glasgow G12 8QQ, Scotland, UK
    Biochem J 370:429-38. 2003
    ..The unique tissue distribution and intracellular targeting of PDE4B4 suggests that this isoform may have a distinct functional role in regulating cAMP levels in specific cell types...
  68. ncbi request reprint TAPAS-1, a novel microdomain within the unique N-terminal region of the PDE4A1 cAMP-specific phosphodiesterase that allows rapid, Ca2+-triggered membrane association with selectivity for interaction with phosphatidic acid
    George S Baillie
    Molecular Pharmacology Group, Division of Biochemistry and Molecular Biology, Wolfson Building, Institute of Biomedical and Life Sciences IBLS, University of Glasgow, Glasgow G12 8QQ, Scotland, United Kingdom
    J Biol Chem 277:28298-309. 2002
    ....
  69. doi request reprint A scanning peptide array approach uncovers association sites within the JNK/beta arrestin signalling complex
    Xiang Li
    Neuroscience and Molecular Pharmacology, Faculty of Biomedical and Life Sciences, Wolfson Building, Glasgow University, Glasgow G12 8QQ, United Kingdom
    FEBS Lett 583:3310-6. 2009
    ....
  70. pmc Expression, intracellular distribution and basis for lack of catalytic activity of the PDE4A7 isoform encoded by the human PDE4A cAMP-specific phosphodiesterase gene
    Lee Ann Johnston
    Molecular Pharmacology Group, Division of Biochemistry and Molecular Biology, Wolfson Building, Institute of Biomedical and Life Sciences, University of Glasgow, Glasgow G12 8QQ, Scotland, UK
    Biochem J 380:371-84. 2004
    ..Three functional regions in PDE4A isoforms are identified, which influence catalytic activity, subcellular targeting and conformational status...
  71. ncbi request reprint The novel long PDE4A10 cyclic AMP phosphodiesterase shows a pattern of expression within brain that is distinct from the long PDE4A5 and short PDE4A1 isoforms
    I McPhee
    Molecular Pharmacology Group, Division of Biochemistry and Molecular Biology, University of Glasgow, Wolfson Building, IBLS, Glasgow G12 8QQ, Scotland, UK
    Cell Signal 13:911-8. 2001
    ..We suggest that specific PDE4A isoforms may have distinct functional roles in the brain, indicating that PDE4A isoform-selective inhibitors may have specific therapeutic and pharmacologic properties...
  72. pmc Molecular cloning of a novel splice variant of human type IVA (PDE-IVA) cyclic AMP phosphodiesterase and localization of the gene to the p13.2-q12 region of human chromosome 19 [corrected]
    Y M Horton
    Division of Biochemistry and Molecular Biology, IBLS, University of Glasgow, U K
    Biochem J 308:683-91. 1995
    ....
  73. ncbi request reprint Determination of the structure of the N-terminal splice region of the cyclic AMP-specific phosphodiesterase RD1 (RNPDE4A1) by 1H NMR and identification of the membrane association domain using chimeric constructs
    K J Smith
    Molecular Pharmacology Group, Division of Biochemistry and Molecular Biology, Institute of Life and Biomedical Sciences, University of Glasgow, Glasgow G12 8QQ, Scotland
    J Biol Chem 271:16703-11. 1996
    ....
  74. pmc Disrupted in schizophrenia 1 and phosphodiesterase 4B: towards an understanding of psychiatric illness
    J Kirsty Millar
    University of Edinburgh, Medical Genetics Section, Molecular Medicine Centre, Crewe Road, Edinburgh EH4 2XU, Scotland, UK
    J Physiol 584:401-5. 2007
    ..Dysregulated cAMP signalling in specific cellular compartments may therefore be a predisposing factor for major mental illness...
  75. doi request reprint p62 (SQSTM1) and cyclic AMP phosphodiesterase-4A4 (PDE4A4) locate to a novel, reversible protein aggregate with links to autophagy and proteasome degradation pathways
    Frank Christian
    Neuroscience and Molecular Pharmacology, Wolfson and Davidson Buildings, Faculty of Biomedical and Life Sciences, University of Glasgow, Glasgow, G12 8QQ, Scotland, United Kingdom
    Cell Signal 22:1576-96. 2010
    ....
  76. ncbi request reprint Intracellular targeting of phosphodiesterase-4 underpins compartmentalized cAMP signaling
    Martin J Lynch
    Division of Biochemistry and Molecular Biology, IBLS, Wolfson Building University of Glasgow, Glasgow G12 8QQ, Scotland, United Kingdom
    Curr Top Dev Biol 75:225-59. 2006
    ..The cAMP cascade has long been linked to cellular growth and embryogenesis and with this comes the implication that PDE4 may play considerable roles in the regulation of progeny development in maturing cells and tissues...
  77. pmc MEK1 binds directly to betaarrestin1, influencing both its phosphorylation by ERK and the timing of its isoprenaline-stimulated internalization
    Dong Meng
    Neuroscience and Molecular Pharmacology, Faculty of Biomedical and Life Sciences, Wolfson Building, University of Glasgow, Glasgow G12 8QQ, Scotland, United Kingdom
    J Biol Chem 284:11425-35. 2009
    ..In addition, the MEK disruptor peptide promoted the ability of betaarrestin1 to co-immunoprecipitate with endogenous c-Src and clathrin, facilitating the isoprenaline-stimulated internalization of the beta(2)-adrenergic receptor...
  78. ncbi request reprint Helix-1 of the cAMP-specific phosphodiesterase PDE4A1 regulates its phospholipase-D-dependent redistribution in response to release of Ca2+
    Elaine Huston
    Molecular Pharmacology Group, Division of Biochemistry and Molecular Biology, Institute of Biomedical and Life Sciences, University of Glasgow, Scotland, UK
    J Cell Sci 119:3799-810. 2006
    ..This study provides the first evidence for Ca2+-triggered relocalisation of a cAMP phosphodiesterase and indicates a potential means for allowing cross-talk between the cAMP, phospholipase D and Ca2+-signalling pathways...
  79. ncbi request reprint In addition to the SH3 binding region, multiple regions within the N-terminal noncatalytic portion of the cAMP-specific phosphodiesterase, PDE4A5, contribute to its intracellular targeting
    Matthew B Beard
    Molecular Pharmacology Group, Division of Biochemistry and Molecular Biology, Davidson Building, Institute of Biomedical and Life Sciences, University of Glasgow, G12 8QQ, Scotland, Glasgow, UK
    Cell Signal 14:453-65. 2002
    ..This suggests that certain of these anchor sites may not only determine intracellular targeting but may also transduce regulatory effects on PDE4A5 activity...
  80. doi request reprint Derivation of endothelial cells from human embryonic stem cells by directed differentiation: analysis of microRNA and angiogenesis in vitro and in vivo
    Nicole M Kane
    British Heart Foundation Glasgow Cardiovascular Research Centre, Faculty of Medicine, University of Glasgow, 126 University Place, Glasgow G12 8TA, UK
    Arterioscler Thromb Vasc Biol 30:1389-97. 2010
    ....
  81. doi request reprint Inferring signaling pathway topologies from multiple perturbation measurements of specific biochemical species
    Tian Rui Xu
    Faculty of Biomedical and Life Sciences, University of Glasgow, Glasgow G128QQ, UK
    Sci Signal 3:ra20. 2010
    ..Thus, our formal methodology rationally infers evidentially supported pathway topologies even when a limited number of biochemical and kinetic measurements are available...
  82. pmc Remodelling of the PDE4 cAMP phosphodiesterase isoform profile upon monocyte-macrophage differentiation of human U937 cells
    Malcolm C Shepherd
    Molecular Pharmacology Group, Division of Biochemistry and Molecular Biology, Institute of Biomedical and Life Sciences, Wolfson Building, University Avenue, University of Glasgow, Glasgow G12 8QQ, Scotland
    Br J Pharmacol 142:339-51. 2004
    ....
  83. ncbi request reprint Fluorescence resonance energy transfer-based analysis of cAMP dynamics in live neonatal rat cardiac myocytes reveals distinct functions of compartmentalized phosphodiesterases
    Marco Mongillo
    Dulbecco Telethon Institute, Padova, Italy
    Circ Res 95:67-75. 2004
    ..PDE3 and PDE4 localize to distinct compartments and this may underpin their different functional roles. Our findings indicate the importance of distinctly localized PDE isoenzymes in determining compartmentalized cAMP signaling...
  84. ncbi request reprint Differential expression of PDE4 cAMP phosphodiesterase isoforms in inflammatory cells of smokers with COPD, smokers without COPD, and nonsmokers
    Rachael Barber
    Respiratory Disease Area, Novartis Horsham Research Center, Wimblehurst Road, Horsham RH12 5AB, United Kingdom
    Am J Physiol Lung Cell Mol Physiol 287:L332-43. 2004
    ..The data obtained suggest that PDE4A4 may be relevant as a macrophage-specific anti-inflammatory target for COPD...
  85. ncbi request reprint The unique amino-terminal region of the PDE4D5 cAMP phosphodiesterase isoform confers preferential interaction with beta-arrestins
    Graeme B Bolger
    University of Alabama at Birmingham, Comprehensive Cancer Center, Birmingham, Alabama 35294 3300, USA
    J Biol Chem 278:49230-8. 2003
    ..This specificity appears likely to account for the preferential recruitment of PDE4D5, compared with PDE4D3, to membranes of Hek-B2 cells and cardiac myocytes upon challenge with isoproterenol...
  86. ncbi request reprint Occupancy of the catalytic site of the PDE4A4 cyclic AMP phosphodiesterase by rolipram triggers the dynamic redistribution of this specific isoform in living cells through a cyclic AMP independent process
    Robert Terry
    BioImage A S, Moerkhoej Bygade 28, Soeborg DK 2860, Denmark
    Cell Signal 15:955-71. 2003
    ..Foci formation of PDE4A4 may be of use in probing for conformational changes in this isoform and for sub-categorising PDE4 selective inhibitors...
  87. ncbi request reprint Chemoresistant KM12C colon cancer cells are addicted to low cyclic AMP levels in a phosphodiesterase 4-regulated compartment via effects on phosphoinositide 3-kinase
    David G McEwan
    The Beatson Institute for Cancer Research, Cancer Research UK Beatson Laboratories, Glasgow, United Kingdom
    Cancer Res 67:5248-57. 2007
    ..Well-tolerated doses of PDE4 inhibitors that are already in clinical development for other therapeutic indications may provide an exciting new strategy for the treatment of colon cancer...
  88. ncbi request reprint Attenuation of the activity of the cAMP-specific phosphodiesterase PDE4A5 by interaction with the immunophilin XAP2
    Graeme B Bolger
    Veterans Affairs Medical Center, Huntsman Cancer Institute, Department of Medicine, Division of Oncology, University of Utah Health Sciences Center, Salt Lake City, Utah 84148, USA
    J Biol Chem 278:33351-63. 2003
    ..We suggest that XAP2 functionally interacts with PDE4A5 in cells...
  89. ncbi request reprint Targeting of cyclic AMP degradation to beta 2-adrenergic receptors by beta-arrestins
    Stephen J Perry
    Howard Hughes Medical Institute, Department of Medicine, Duke University Medical Center, Durham, NC 27710, USA
    Science 298:834-6. 2002
    ....
  90. doi request reprint Protein kinase A type I and type II define distinct intracellular signaling compartments
    Giulietta Di Benedetto
    Dulbecco Telethon Institute, Venetian Institute of Molecular Medicine, Padova, Italy
    Circ Res 103:836-44. 2008
    ..The selective activation of individual PKA isoforms thus leads to phosphorylation of unique subsets of downstream targets...
  91. ncbi request reprint Compartmentalized phosphodiesterase-2 activity blunts beta-adrenergic cardiac inotropy via an NO/cGMP-dependent pathway
    Marco Mongillo
    Dulbecco Telethon Institute, Venetian Institute of Molecular Medicine, Padova, Italy
    Circ Res 98:226-34. 2006
    ..Our study illustrates the key role of compartmentalized PDE2 in the control of catecholamine-generated cAMP and furthers our understanding of localized cAMP signaling...
  92. pmc p75 neurotrophin receptor regulates tissue fibrosis through inhibition of plasminogen activation via a PDE4/cAMP/PKA pathway
    Benjamin D Sachs
    Department of Pharmacology, University of California, San Diego, La Jolla, CA 92093, USA
    J Cell Biol 177:1119-32. 2007
    ..Our results reveal a novel pathogenic mechanism by which p75(NTR) regulates degradation of cAMP and perpetuates scar formation after injury...
  93. ncbi request reprint Compartmentalization of cAMP-dependent signaling by phosphodiesterase-4D is involved in the regulation of vasopressin-mediated water reabsorption in renal principal cells
    Eduard Stefan
    Leibniz Institut fur Molekulare Pharmakologie, Berlin, Germany
    J Am Soc Nephrol 18:199-212. 2007
    ..Taken together, a novel, compartmentalized, and physiologically relevant cAMP-dependent signal transduction module on AQP2-bearing vesicles, comprising anchored PDE4D, AKAP18delta, and PKA, has been identified...
  94. pmc PGE(1) stimulation of HEK293 cells generates multiple contiguous domains with different [cAMP]: role of compartmentalized phosphodiesterases
    Anna Terrin
    Dulbecco Telethon Institute, Venetian Institute of Molecular Medicine, 35129 Padova, Italy
    J Cell Biol 175:441-51. 2006
    ....
  95. ncbi request reprint 1H NMR structural and functional characterisation of a cAMP-specific phosphodiesterase-4D5 (PDE4D5) N-terminal region peptide that disrupts PDE4D5 interaction with the signalling scaffold proteins, beta-arrestin and RACK1
    K John Smith
    School of Biosciences, University of Birmingham, Edgbaston, Birmingham, PO Box 363, B15 2TT, UK
    Cell Signal 19:2612-24. 2007
    ....
  96. ncbi request reprint Reduced PDE4 expression and activity contributes to enhanced catecholamine-induced cAMP accumulation in adipocytes from FOXC2 transgenic mice
    Line M Grønning
    Biotechnology Centre of Oslo, University of Oslo, P O Box 1125 Blindern, 0317 Oslo, Norway
    FEBS Lett 580:4126-30. 2006
    ..Thus, reduced PDE4 activity in adipocytes from FOXC2 transgenic mice contributes to amplified beta-AR induced cAMP responses observed in these cells...
  97. ncbi request reprint Dynamic regulation, desensitization, and cross-talk in discrete subcellular microdomains during beta2-adrenoceptor and prostanoid receptor cAMP signaling
    Debbie Willoughby
    Department of Pharmacology, University of Cambridge, Tennis Court Road, Cambridge, UK
    J Biol Chem 282:34235-49. 2007
    ....
  98. pmc Constitutive activation of the G-protein subunit Galphas within forebrain neurons causes PKA-dependent alterations in fear conditioning and cortical Arc mRNA expression
    Michele P Kelly
    Department of Biology, University of Pennsylvania, Philadelphia, Pennsylvania 19104, USA
    Learn Mem 15:75-83. 2008
    ..Our results imply that chronically stimulating targets upstream of cAMP may detrimentally affect cognition...
  99. pmc Scanning peptide array analyses identify overlapping binding sites for the signalling scaffold proteins, beta-arrestin and RACK1, in cAMP-specific phosphodiesterase PDE4D5
    Graeme B Bolger
    Comprehensive Cancer Center, University of Alabama at Birmingham, Birmingham, AL 35294 3300, USA
    Biochem J 398:23-36. 2006
    ..In this fashion, alterations in the level of RACK1 expression may act to modulate signal transduction mediated by the beta2AR...
  100. ncbi request reprint Phosphorylation-dependent interactions between ADAM15 cytoplasmic domain and Src family protein-tyrosine kinases
    Zaruhi Poghosyan
    School of Biological Sciences, University of East Anglia, Norwich NR4 7TJ, United Kingdom
    J Biol Chem 277:4999-5007. 2002
    ..These data demonstrate selective, phosphorylation-dependent interactions of ADAM15 with Src family PTKs and Grb2, which highlight the potential for integration of ADAM functions and cellular signaling...
  101. ncbi request reprint DISC1 and PDE4B are interacting genetic factors in schizophrenia that regulate cAMP signaling
    J Kirsty Millar
    Medical Genetics Section, Molecular Medicine Centre, University of Edinburgh, Edinburgh EH4 2XU, UK
    Science 310:1187-91. 2005
    ..We propose a mechanistic model whereby DISC1 sequesters PDE4B in resting cells and releases it in an activated state in response to elevated cAMP...