Florian Hollfelder

Summary

Affiliation: University of Cambridge
Country: UK

Publications

  1. ncbi request reprint SNAP Display - an In Vitro Method for the Selection of Protein Binders
    Gillian Houlihan
    Department of Biochemistry, University of Cambridge, 80 Tennis Court Road, Cambridge CB2 1GA, UK
    Curr Pharm Des 19:5421-8. 2013
  2. pmc A single mutation in the core domain of the lac repressor reduces leakiness
    Pietro Gatti-Lafranconi
    Department of Biochemistry, University of Cambridge, Cambridge CB2 1GA, UK
    Microb Cell Fact 12:67. 2013
  3. ncbi request reprint A simple method to evaluate the biochemical compatibility of oil/surfactant mixtures for experiments in microdroplets
    Miriam Kaltenbach
    Department of Biochemistry, University of Cambridge, 80 Tennis Court Road, CB2 1GA, Cambridge, UK
    Lab Chip 12:4185-92. 2012
  4. doi request reprint Microdroplets: a sea of applications?
    Ansgar Huebner
    Department of Chemistry, Lensfield Road, Cambridge, UKCB2 1EW
    Lab Chip 8:1244-54. 2008
  5. doi request reprint Controlling the contents of microdroplets by exploiting the permeability of PDMS
    Jung uk Shim
    Department of Chemistry, University of Cambridge, Cambridge, UK
    Lab Chip 11:1132-7. 2011

Collaborators

  • Ansgar Huebner
  • David R Spring
  • Tom L Blundell
  • Gillian Houlihan
  • Pietro Gatti-Lafranconi
  • Sean R A Devenish
  • Miriam Kaltenbach
  • Jung uk Shim
  • Willem P Dijkman
  • David Lowe
  • Martin Welch
  • Santoshkumar N Patil
  • Steven D Bowden
  • Chris Abell
  • Wilhelm T S Huck
  • James T Hodgkinson

Detail Information

Publications5

  1. ncbi request reprint SNAP Display - an In Vitro Method for the Selection of Protein Binders
    Gillian Houlihan
    Department of Biochemistry, University of Cambridge, 80 Tennis Court Road, Cambridge CB2 1GA, UK
    Curr Pharm Des 19:5421-8. 2013
    ..This review describes the principles of SNAP display and extrapolates its scope for in vitro selections. ..
  2. pmc A single mutation in the core domain of the lac repressor reduces leakiness
    Pietro Gatti-Lafranconi
    Department of Biochemistry, University of Cambridge, Cambridge CB2 1GA, UK
    Microb Cell Fact 12:67. 2013
    ..However, the leakiness of LacI that is essential for the natural function of the lac operon leads to an increased energetic burden, and potentially toxicity, in heterologous protein production...
  3. ncbi request reprint A simple method to evaluate the biochemical compatibility of oil/surfactant mixtures for experiments in microdroplets
    Miriam Kaltenbach
    Department of Biochemistry, University of Cambridge, 80 Tennis Court Road, CB2 1GA, Cambridge, UK
    Lab Chip 12:4185-92. 2012
    ..This methodology will serve as a quantitative test for the rapid optimization of droplet-based experiments such as in vitro protein expression or enzymatic assays...
  4. doi request reprint Microdroplets: a sea of applications?
    Ansgar Huebner
    Department of Chemistry, Lensfield Road, Cambridge, UKCB2 1EW
    Lab Chip 8:1244-54. 2008
    ..Furthermore, we discuss key fields of use in which such systems may make a significant impact, with particular emphasis on novel applications in the biological and physical sciences...
  5. doi request reprint Controlling the contents of microdroplets by exploiting the permeability of PDMS
    Jung uk Shim
    Department of Chemistry, University of Cambridge, Cambridge, UK
    Lab Chip 11:1132-7. 2011
    ..Likewise quorum sensing in bacteria was triggered by delivery of the auto-inducer N-(3-oxododecanoyl)-l-homoserine lactone (OdDHL) through the PDMS membrane of the device...