Johannes Hohlbein

Summary

Affiliation: University of Oxford
Country: UK

Publications

  1. doi request reprint Three-dimensional orientation determination of the emission dipoles of single molecules: the shot-noise limit
    Johannes Hohlbein
    Max Planck Institute of Microstructure Physics, Weinberg 2, 06120 Halle, Germany
    J Chem Phys 129:094703. 2008
  2. doi request reprint Surfing on a new wave of single-molecule fluorescence methods
    Johannes Hohlbein
    Department of Physics, Biological Physics Research Group, University of Oxford, Oxford, UK
    Phys Biol 7:031001. 2010
  3. pmc Identifying molecular dynamics in single-molecule FRET experiments with burst variance analysis
    Joseph P Torella
    Department of Physics and Biological Physics Research Group, University of Oxford, Oxford, United Kingdom
    Biophys J 100:1568-77. 2011
  4. pmc Characterization of dark quencher chromophores as nonfluorescent acceptors for single-molecule FRET
    Ludovic Le Reste
    Biological Physics Research Group, Department of Physics, University of Oxford, Oxford, United Kingdom
    Biophys J 102:2658-68. 2012
  5. pmc Conformational landscapes of DNA polymerase I and mutator derivatives establish fidelity checkpoints for nucleotide insertion
    Johannes Hohlbein
    Biological Physics Research Group, Department of Physics, Clarendon Laboratory, University of Oxford, Parks Road, Oxford OX1 3PU, UK
    Nat Commun 4:2131. 2013
  6. pmc Defining the limits of single-molecule FRET resolution in TIRF microscopy
    Seamus J Holden
    Biological Physics Research Group, Department of Physics, University of Oxford, Oxford, United Kingdom
    Biophys J 99:3102-11. 2010
  7. pmc Conformational transitions in DNA polymerase I revealed by single-molecule FRET
    Yusdi Santoso
    Biological Physics Research Group, Department of Physics, Clarendon Laboratory, University of Oxford, Oxford OX1 3PU, United Kingdom
    Proc Natl Acad Sci U S A 107:715-20. 2010

Collaborators

Detail Information

Publications7

  1. doi request reprint Three-dimensional orientation determination of the emission dipoles of single molecules: the shot-noise limit
    Johannes Hohlbein
    Max Planck Institute of Microstructure Physics, Weinberg 2, 06120 Halle, Germany
    J Chem Phys 129:094703. 2008
    ..The simulations and experimental data highlight the potential of our method and give practical guidelines for its application...
  2. doi request reprint Surfing on a new wave of single-molecule fluorescence methods
    Johannes Hohlbein
    Department of Physics, Biological Physics Research Group, University of Oxford, Oxford, UK
    Phys Biol 7:031001. 2010
    ....
  3. pmc Identifying molecular dynamics in single-molecule FRET experiments with burst variance analysis
    Joseph P Torella
    Department of Physics and Biological Physics Research Group, University of Oxford, Oxford, United Kingdom
    Biophys J 100:1568-77. 2011
    ....
  4. pmc Characterization of dark quencher chromophores as nonfluorescent acceptors for single-molecule FRET
    Ludovic Le Reste
    Biological Physics Research Group, Department of Physics, University of Oxford, Oxford, United Kingdom
    Biophys J 102:2658-68. 2012
    ....
  5. pmc Conformational landscapes of DNA polymerase I and mutator derivatives establish fidelity checkpoints for nucleotide insertion
    Johannes Hohlbein
    Biological Physics Research Group, Department of Physics, Clarendon Laboratory, University of Oxford, Parks Road, Oxford OX1 3PU, UK
    Nat Commun 4:2131. 2013
    ..The mutator phenotype of the low-fidelity derivatives correlates well with reduced affinity for complementary dNTPs and highlights the partially closed conformation as a primary checkpoint for nucleotide selection. ..
  6. pmc Defining the limits of single-molecule FRET resolution in TIRF microscopy
    Seamus J Holden
    Biological Physics Research Group, Department of Physics, University of Oxford, Oxford, United Kingdom
    Biophys J 99:3102-11. 2010
    ..the mechanisms of which are often characterized by distance changes on the scale of one DNA basepair...
  7. pmc Conformational transitions in DNA polymerase I revealed by single-molecule FRET
    Yusdi Santoso
    Biological Physics Research Group, Department of Physics, Clarendon Laboratory, University of Oxford, Oxford OX1 3PU, United Kingdom
    Proc Natl Acad Sci U S A 107:715-20. 2010
    ..Ternary complexes formed in the presence of mismatched dNTPs or complementary ribonucleotides show unique FRET species, which we suggest are relevant to kinetic checkpoints that discriminate against these incorrect substrates...