Jennifer Hirst

Summary

Affiliation: University of Cambridge
Country: UK

Publications

  1. pmc Interaction between AP-5 and the hereditary spastic paraplegia proteins SPG11 and SPG15
    Jennifer Hirst
    Cambridge Institute for Medical Research, University of Cambridge, Cambridge CB2 0XY, United Kingdom
    Mol Biol Cell 24:2558-69. 2013
  2. doi request reprint Adaptor protein complexes AP-4 and AP-5: new players in endosomal trafficking and progressive spastic paraplegia
    Jennifer Hirst
    Cambridge Institute for Medical Research, University of Cambridge, Wellcome Trust MRC Building, Cambridge, CB2 0XY, UK
    Traffic 14:153-64. 2013
  3. pmc Distinct and overlapping roles for AP-1 and GGAs revealed by the "knocksideways" system
    Jennifer Hirst
    University of Cambridge, Cambridge Institute for Medical Research, Cambridge CB2 0XY, UK
    Curr Biol 22:1711-6. 2012
  4. ncbi request reprint Clathrin and adaptors
    J Hirst
    Department of Clinical Biochemistry, University of Cambridge, Addenbrooke s Hospital, Hills Road, Cambridge CB2 2QR, UK
    Biochim Biophys Acta 1404:173-93. 1998
  5. pmc Auxilin depletion causes self-assembly of clathrin into membraneless cages in vivo
    Jennifer Hirst
    Cambridge Institute for Medical Research, University of Cambridge, Wellcome Trust, Addenbrooke s Hospital, Cambridge, UK
    Traffic 9:1354-71. 2008
  6. ncbi request reprint Spatial and functional relationship of GGAs and AP-1 in Drosophila and HeLa cells
    Jennifer Hirst
    University of Cambridge, Cambridge Institute for Medical Research, Cambridge CB2 0XY, UK
    Traffic 10:1696-710. 2009
  7. pmc A potential role for the clathrin adaptor GGA in Drosophila spermatogenesis
    Jennifer Hirst
    University of Cambridge, Cambridge Institute for Medical Research, Cambridge, UK
    BMC Cell Biol 12:22. 2011
  8. pmc The aftiphilin/p200/gamma-synergin complex
    Jennifer Hirst
    Department of Clinical Biochemistry, Cambridge Institute for Medical Research, University of Cambridge, Cambridge CB2 2XY, United Kingdom
    Mol Biol Cell 16:2554-65. 2005
  9. pmc The role of cargo proteins in GGA recruitment
    Jennifer Hirst
    Cambridge Institute for Medical Research, University of Cambridge, Cambridge CB2 0XY, UK
    Traffic 8:594-604. 2007
  10. pmc Functional analysis of AP-2 alpha and mu2 subunits
    Alison M Motley
    Cambridge Institute for Medical Research, University of Cambridge, Cambridge CB2 2XY, United Kingdom
    Mol Biol Cell 17:5298-308. 2006

Collaborators

Detail Information

Publications22

  1. pmc Interaction between AP-5 and the hereditary spastic paraplegia proteins SPG11 and SPG15
    Jennifer Hirst
    Cambridge Institute for Medical Research, University of Cambridge, Cambridge CB2 0XY, United Kingdom
    Mol Biol Cell 24:2558-69. 2013
    ....
  2. doi request reprint Adaptor protein complexes AP-4 and AP-5: new players in endosomal trafficking and progressive spastic paraplegia
    Jennifer Hirst
    Cambridge Institute for Medical Research, University of Cambridge, Wellcome Trust MRC Building, Cambridge, CB2 0XY, UK
    Traffic 14:153-64. 2013
    ..The unexpected phenotypic parallels between AP-4 and AP-5 patients may in turn suggest a functional relationship of the two APs in vesicle trafficking...
  3. pmc Distinct and overlapping roles for AP-1 and GGAs revealed by the "knocksideways" system
    Jennifer Hirst
    University of Cambridge, Cambridge Institute for Medical Research, Cambridge CB2 0XY, UK
    Curr Biol 22:1711-6. 2012
    ..Our study shows that knocksideways and proteomics are a powerful combination for investigating protein function, which can potentially be used on many different types of proteins...
  4. ncbi request reprint Clathrin and adaptors
    J Hirst
    Department of Clinical Biochemistry, University of Cambridge, Addenbrooke s Hospital, Hills Road, Cambridge CB2 2QR, UK
    Biochim Biophys Acta 1404:173-93. 1998
    ..Genetic studies indicate that AP-3 plays a role in the sorting of proteins to lysosomes and lysosome-related organelles...
  5. pmc Auxilin depletion causes self-assembly of clathrin into membraneless cages in vivo
    Jennifer Hirst
    Cambridge Institute for Medical Research, University of Cambridge, Wellcome Trust, Addenbrooke s Hospital, Cambridge, UK
    Traffic 9:1354-71. 2008
    ..Together, these findings indicate that the two auxilins have overlapping functions and that they not only facilitate the uncoating of CCVs but also prevent the formation of nonproductive clathrin cages in the cytosol...
  6. ncbi request reprint Spatial and functional relationship of GGAs and AP-1 in Drosophila and HeLa cells
    Jennifer Hirst
    University of Cambridge, Cambridge Institute for Medical Research, Cambridge CB2 0XY, UK
    Traffic 10:1696-710. 2009
    ..Thus, our results indicate that the two adaptors can function independently of each other...
  7. pmc A potential role for the clathrin adaptor GGA in Drosophila spermatogenesis
    Jennifer Hirst
    University of Cambridge, Cambridge Institute for Medical Research, Cambridge, UK
    BMC Cell Biol 12:22. 2011
    ..Thus Drosophila melanogaster is a useful model system to study tissue expression profiles and knockdown phenotypes as there is a single GGA ortholog...
  8. pmc The aftiphilin/p200/gamma-synergin complex
    Jennifer Hirst
    Department of Clinical Biochemistry, Cambridge Institute for Medical Research, University of Cambridge, Cambridge CB2 2XY, United Kingdom
    Mol Biol Cell 16:2554-65. 2005
    ....
  9. pmc The role of cargo proteins in GGA recruitment
    Jennifer Hirst
    Cambridge Institute for Medical Research, University of Cambridge, Cambridge CB2 0XY, UK
    Traffic 8:594-604. 2007
    ..Together, these results demonstrate that cargo proteins contribute to the recruitment of GGAs onto membranes and to the formation of GGA-positive CCVs...
  10. pmc Functional analysis of AP-2 alpha and mu2 subunits
    Alison M Motley
    Cambridge Institute for Medical Research, University of Cambridge, Cambridge CB2 2XY, United Kingdom
    Mol Biol Cell 17:5298-308. 2006
    ..Finally, they establish a new model system that can be used both for additional structure-function analyses, and as a way of testing tagged constructs for function in vivo...
  11. pmc EpsinR is an adaptor for the SNARE protein Vti1b
    Jennifer Hirst
    University of Cambridge, Cambridge Institute for Medical Research, Cambridge CB2 2XY, United Kingdom
    Mol Biol Cell 15:5593-602. 2004
    ..These findings provide in vivo evidence that epsinR is an adaptor for vti1b, and they also show that CCV isolation can be used as an assay for adaptor function...
  12. pmc The fifth adaptor protein complex
    Jennifer Hirst
    University of Cambridge, Cambridge Institute for Medical Research, Cambridge, UK
    PLoS Biol 9:e1001170. 2011
    ..Thus, AP-5 is an evolutionarily ancient complex, which is involved in endosomal sorting, and which has links with hereditary spastic paraplegia...
  13. pmc EpsinR: an ENTH domain-containing protein that interacts with AP-1
    Jennifer Hirst
    University of Cambridge, Department of Clinical Biochemistry, Cambridge Institute for Medical Research, United Kingdom
    Mol Biol Cell 14:625-41. 2003
    ..This indicates that although epsinR is likely to be an important component of the AP-1 network, it is not necessary for the sorting of lysosomal enzymes...
  14. pmc Binding partners for the COOH-terminal appendage domains of the GGAs and gamma-adaptin
    Winnie W Y Lui
    Department of Clinical Biochemistry, Cambridge Institute for Medical Research, University of Cambridge, United Kingdom
    Mol Biol Cell 14:2385-98. 2003
    ..By overexpressing wild-type GGA and gamma appendage domains in cells, we can drive p56 and gamma-synergin, respectively, into the cytosol, suggesting a possible mechanism for selectively disrupting the two pathways...
  15. pmc Multivariate proteomic profiling identifies novel accessory proteins of coated vesicles
    Georg H H Borner
    Cambridge Institute for Medical Research, Wellcome Trust Medical Research Council Building, University of Cambridge, Cambridge CB2 0XY, England, UK
    J Cell Biol 197:141-60. 2012
    ..The profiling approach can be adapted to address related cell and systems biological questions...
  16. ncbi request reprint Rapid inactivation of proteins by knocksideways
    Margaret S Robinson
    Cambridge Institute for Medical Research, University of Cambridge, Cambridge, United Kingdom
    Curr Protoc Cell Biol 61:15.20.1-7. 2013
    ..Curr. Protoc. Cell Biol. 61:15.20.1-15.20.7. © 2013 by John Wiley & Sons, Inc. ..
  17. pmc GGAs: roles of the different domains and comparison with AP-1 and clathrin
    J Hirst
    University of Cambridge, Department of Clinical Biochemistry, Wellcome Trust Centre for the Study of Molecular Mechanisms in Disease, Cambridge CB2 2XY, United Kingdom
    Mol Biol Cell 12:3573-88. 2001
    ..Together with results from other studies, our findings suggest that the GGAs act as monomeric adaptors, with the four domains involved in cargo selection, membrane localization, clathrin binding, and accessory protein recruitment...
  18. ncbi request reprint Energy transduction by respiratory complex I--an evaluation of current knowledge
    J Hirst
    Medical Research Council Dunn Human Nutrition Unit, Wellcome Trust MRC Building, Hills Road, Cambridge CB2 2XY, UK
    Biochem Soc Trans 33:525-9. 2005
    ..Here, I aim to describe and examine data relating to the mechanism of complex I and to build a framework to facilitate the discussion of possible conclusions and mechanistic models...
  19. ncbi request reprint GRIM-19, a cell death regulatory gene product, is a subunit of bovine mitochondrial NADH:ubiquinone oxidoreductase (complex I)
    I M Fearnley
    Medical Research Council Dunn Human Nutrition Unit, Wellcome Trust Medical Research Council Building, Hills Road, Cambridge CB2 2XY, United Kingdom
    J Biol Chem 276:38345-8. 2001
    ..6) is the bovine homolog of GRIM-19, the product of a cell death regulatory gene induced by interferon-beta and retinoic acid, thus providing a new link between the mitochondrion and its electron-transport chain and apoptotic cell death...
  20. ncbi request reprint Atomically defined mechanism for proton transfer to a buried redox centre in a protein
    K Chen
    Department of Molecular Biology and Biochemistry, University of California, Irvine 92612, USA
    Nature 405:814-7. 2000
    ..Our analysis defines the structural, dynamic and energetic requirements for proton courier groups in redox-driven proton-pumping enzymes...
  21. pmc A family of proteins with gamma-adaptin and VHS domains that facilitate trafficking between the trans-Golgi network and the vacuole/lysosome
    J Hirst
    University of Cambridge, Department of Clinical Biochemistry, Wellcome Trust Centre for the Study of Molecular Mechanisms in Disease, Cambridge CB2 2XY, United Kingdom
    J Cell Biol 149:67-80. 2000
    ..These results indicate that the function of the GGAs is to facilitate the trafficking of proteins between the TGN and the vacuole, or its mammalian equivalent, the lysosome...
  22. pmc Characterization of a fourth adaptor-related protein complex
    J Hirst
    University of Cambridge, Department of Clinical Biochemistry and Cambridge Institute for Medical Research, Cambridge CB2 2XY, England
    Mol Biol Cell 10:2787-802. 1999
    ..AP-4 is of relatively low abundance, but it is expressed ubiquitously, suggesting that it participates in a specialized trafficking pathway but one that is required in all cell types...