C J Hewitt

Summary

Affiliation: University of Birmingham
Country: UK

Publications

  1. ncbi request reprint The application of multi-parameter flow cytometry to the study of recombinant Escherichia coli batch fermentation processes
    Gareth Lewis
    School of Engineering Chemical Engineering, The University of Birmingham, B15 2TT, Edgbaston, UK
    J Ind Microbiol Biotechnol 31:311-22. 2004
  2. ncbi request reprint The use of flow cytometry to study the impact of fluid mechanical stress on Escherichia coli W3110 during continuous cultivation in an agitated bioreactor
    C J Hewitt
    Centre for Bioprocess Engineering, School of Chemical Engineering, The University of Birmingham, Edgbaston, B15 2TT, United Kingdom
    Biotechnol Bioeng 59:612-20. 1998
  3. ncbi request reprint Use of multi-staining flow cytometry to characterise the physiological state of Escherichia coli W3110 in high cell density fed-batch cultures
    C J Hewitt
    Centre for Bioprocess Engineering, School of Chemical Engineering, The University of Birmingham, Edgbaston, B15 2TT, U K
    Biotechnol Bioeng 63:705-11. 1999
  4. ncbi request reprint The use of multi-parameter flow cytometry to compare the physiological response of Escherichia coli W3110 to glucose limitation during batch, fed-batch and continuous culture cultivations
    C J Hewitt
    Centre for Bioprocess Engineering, School of Chemical Engineering, University of Birmingham, Edgbaston, UK
    J Biotechnol 75:251-64. 1999
  5. ncbi request reprint An industrial application of multiparameter flow cytometry: assessment of cell physiological state and its application to the study of microbial fermentations
    C J Hewitt
    Centre for Bioprocess Engineering, School of Chemical Engineering, The University of Birmingham, Edgbaston, United Kingdom
    Cytometry 44:179-87. 2001
  6. ncbi request reprint A study into the anti-microbial properties of an amino functionalised polymer using multi-parameter flow cytometry
    Christopher J Hewitt
    School of Engineering Chemical Engineering, Biochemical Engineering, The University of Birmingham, Edgbaston, B15 2TT, UK
    Biotechnol Lett 26:549-57. 2004
  7. ncbi request reprint The application of multi-parameter flow cytometry to monitor individual microbial cell physiological state
    Christopher J Hewitt
    Centre for Formulation Engineering, Biochemical Engineering, School of Engineering Chemical Engineering, The University of Birmingham, Edgbaston B15 2TT, UK
    Adv Biochem Eng Biotechnol 89:197-223. 2004
  8. ncbi request reprint Characterization of Acanthamoeba-microsphere association by multiparameter flow cytometry and confocal microscopy
    Edward A G Elloway
    Life and Health Sciences, Aston University, Birmingham, United Kingdom
    Cytometry A 69:266-72. 2006
  9. ncbi request reprint Monitoring and quantification of inclusion body formation in Escherichia coli by multi-parameter flow cytometry
    Fredrik Wallberg
    Department of Biotechnology, Royal Institute of Technology, Roslagstullsbacken 21, S 10691 Stockholm, Sweden
    Biotechnol Lett 27:919-26. 2005
  10. ncbi request reprint The impact of fluid mechanical stress on Corynebacterium glutamicum during continuous cultivation in an agitated bioreactor
    Saethawat Chamsartra
    BBERG Bangsaen Biochemical Engineering Research Group, Department of Biology, Faculty of Science, Burapha University, Chon Buri, 20131, Thailand
    Biotechnol Lett 27:693-700. 2005

Collaborators

  • Fredrik Wallberg
  • Heléne Sundström
  • Alvin W Nienow
  • Edward A G Elloway
  • Alberto Reis
  • Saethawat Chamsartra
  • Erika Ledung
  • Sven Olof Enfors
  • Gareth Lewis
  • Helen Onyeaka
  • Steven L Kelly
  • Roger A Bird
  • Stephen N Smith
  • Teresa Lopes da Silva
  • Maria Kosseva
  • J Carlos Roseiro
  • Christopher A Kent
  • Bo Norrman
  • Ian W Taylor

Detail Information

Publications14

  1. ncbi request reprint The application of multi-parameter flow cytometry to the study of recombinant Escherichia coli batch fermentation processes
    Gareth Lewis
    School of Engineering Chemical Engineering, The University of Birmingham, B15 2TT, Edgbaston, UK
    J Ind Microbiol Biotechnol 31:311-22. 2004
    ..Further, we discuss the possibility of optimising inclusion body formation by manipulating the fermentation conditions based on these rapid "real-time" measurements...
  2. ncbi request reprint The use of flow cytometry to study the impact of fluid mechanical stress on Escherichia coli W3110 during continuous cultivation in an agitated bioreactor
    C J Hewitt
    Centre for Bioprocess Engineering, School of Chemical Engineering, The University of Birmingham, Edgbaston, B15 2TT, United Kingdom
    Biotechnol Bioeng 59:612-20. 1998
    ..coli cell line due to variations in agitation or aeration intensity or scale of operation cannot be attributed to fluid dynamic stresses associated with the turbulence generated by impellers or with bursting bubbles...
  3. ncbi request reprint Use of multi-staining flow cytometry to characterise the physiological state of Escherichia coli W3110 in high cell density fed-batch cultures
    C J Hewitt
    Centre for Bioprocess Engineering, School of Chemical Engineering, The University of Birmingham, Edgbaston, B15 2TT, U K
    Biotechnol Bioeng 63:705-11. 1999
    ....
  4. ncbi request reprint The use of multi-parameter flow cytometry to compare the physiological response of Escherichia coli W3110 to glucose limitation during batch, fed-batch and continuous culture cultivations
    C J Hewitt
    Centre for Bioprocess Engineering, School of Chemical Engineering, University of Birmingham, Edgbaston, UK
    J Biotechnol 75:251-64. 1999
    ..Such effects were not found in either batch or continuous culture cultivations. The possibility of using these findings for improved process control using 'on-line' flow cytometry are discussed...
  5. ncbi request reprint An industrial application of multiparameter flow cytometry: assessment of cell physiological state and its application to the study of microbial fermentations
    C J Hewitt
    Centre for Bioprocess Engineering, School of Chemical Engineering, The University of Birmingham, Edgbaston, United Kingdom
    Cytometry 44:179-87. 2001
    ..Therefore, analytical techniques, decoupled from postsampling growth, are desirable to rapidly monitor individual cell physiologic states during microbial fermentations...
  6. ncbi request reprint A study into the anti-microbial properties of an amino functionalised polymer using multi-parameter flow cytometry
    Christopher J Hewitt
    School of Engineering Chemical Engineering, Biochemical Engineering, The University of Birmingham, Edgbaston, B15 2TT, UK
    Biotechnol Lett 26:549-57. 2004
    ..The microbiocidal activity of this polymer may be similar to that of substances referred to as polycationic, amphipathic compounds (peptides, peptide derivatives and other polyamines)...
  7. ncbi request reprint The application of multi-parameter flow cytometry to monitor individual microbial cell physiological state
    Christopher J Hewitt
    Centre for Formulation Engineering, Biochemical Engineering, School of Engineering Chemical Engineering, The University of Birmingham, Edgbaston B15 2TT, UK
    Adv Biochem Eng Biotechnol 89:197-223. 2004
    ....
  8. ncbi request reprint Characterization of Acanthamoeba-microsphere association by multiparameter flow cytometry and confocal microscopy
    Edward A G Elloway
    Life and Health Sciences, Aston University, Birmingham, United Kingdom
    Cytometry A 69:266-72. 2006
    ..Acanthamoebae, in common with other protozoa, readily endocytose particulate material, which in turn may lead to the spread of infectious disease...
  9. ncbi request reprint Monitoring and quantification of inclusion body formation in Escherichia coli by multi-parameter flow cytometry
    Fredrik Wallberg
    Department of Biotechnology, Royal Institute of Technology, Roslagstullsbacken 21, S 10691 Stockholm, Sweden
    Biotechnol Lett 27:919-26. 2005
    ....
  10. ncbi request reprint The impact of fluid mechanical stress on Corynebacterium glutamicum during continuous cultivation in an agitated bioreactor
    Saethawat Chamsartra
    BBERG Bangsaen Biochemical Engineering Research Group, Department of Biology, Faculty of Science, Burapha University, Chon Buri, 20131, Thailand
    Biotechnol Lett 27:693-700. 2005
    ....
  11. ncbi request reprint Monitoring population dynamics of the thermophilic Bacillus licheniformis CCMI 1034 in batch and continuous cultures using multi-parameter flow cytometry
    Alberto Reis
    Instituto Nacional de Engenharia e Tecnologia Industrial, Departamento de Biotecnologia, Estrada do Paco do Lumiar, 22, 1649 038 Lisboa codex, Portugal
    J Biotechnol 115:199-210. 2005
    ..Such information could be used to enhance process efficiency...
  12. ncbi request reprint Segregation to non-dividing cells in recombinant Escherichia coli fed-batch fermentation processes
    Heléne Sundström
    Department of Biotechnology, Royal Institute of Technology, Roslagstullsbacken 21, S 10691 Stockholm, Sweden
    Biotechnol Lett 26:1533-9. 2004
    ..Thus, the drop in the number of c.f.u. ml(-1) probably reflects a loss of cell division capability rather than cell death...
  13. ncbi request reprint Further studies related to the scale-up of high cell density Escherichia coli fed-batch fermentations: the additional effect of a changing microenvironment when using aqueous ammonia to control pH
    Helen Onyeaka
    Biochemical Engineering, Centre for Formulation Engineering, School of Engineering Chemical Engineering, The University of Birmingham, Edgbaston B15 2TT, UK
    Biotechnol Bioeng 84:474-84. 2003
    ....
  14. ncbi request reprint A comparison of high cell density fed-batch fermentations involving both induced and non-induced recombinant Escherichia coli under well-mixed small-scale and simulated poorly mixed large-scale conditions
    Christopher J Hewitt
    Biochemical Engineering, Centre for Formulation Engineering, School of Engineering Chemical Engineering, The University of Birmingham, Edgbaston B15 2TT, United Kingdom
    Biotechnol Bioeng 96:495-505. 2007
    ....