R C Hardie

Summary

Affiliation: University of Cambridge
Country: UK

Publications

  1. ncbi Vision: dynamic platforms
    Roger C Hardie
    Nature 450:37-9. 2007
  2. ncbi Functional equivalence of native light-sensitive channels in the Drosophila trp301 mutant and TRPL cation channels expressed in a stably transfected Drosophila cell line
    R C Hardie
    Department of Anatomy, Cambridge University, UK
    Cell Calcium 21:431-40. 1997
  3. ncbi Activation of heterologously expressed Drosophila TRPL channels: Ca2+ is not required and InsP3 is not sufficient
    R C Hardie
    Department of Anatomy, Cambridge University, UK
    Cell Calcium 24:153-63. 1998
  4. ncbi Phototransduction: shedding light on translocation
    Roger C Hardie
    Cambridge University Department of Anatomy, Downing Street, Cambridge CB2 3DY, UK
    Curr Biol 13:R775-7. 2003
  5. ncbi In vivo light-induced and basal phospholipase C activity in Drosophila photoreceptors measured with genetically targeted phosphatidylinositol 4,5-bisphosphate-sensitive ion channels (Kir2.1)
    Roger C Hardie
    Department of Anatomy, Cambridge University, Downing St, Cambridge CB2 3DY, United Kingdom
    J Biol Chem 279:47773-82. 2004
  6. ncbi Calcium influx via TRP channels is required to maintain PIP2 levels in Drosophila photoreceptors
    R C Hardie
    Department of Anatomy, Cambridge, United Kingdom
    Neuron 30:149-59. 2001
  7. ncbi Visual transduction in Drosophila
    R C Hardie
    Department of Anatomy, University of Cambridge, Downing Street, Cambridge CB2 3DY, UK
    Nature 413:186-93. 2001
  8. ncbi Phototransduction in Drosophila melanogaster
    R C Hardie
    Cambridge University, Department of Anatomy, Downing Street, Cambridge CB2 3DY, UK
    J Exp Biol 204:3403-9. 2001
  9. ncbi TRP channels in Drosophila photoreceptors: the lipid connection
    Roger C Hardie
    Department of Anatomy, Cambridge University, Downing Street, Cambridge CB2 3DY, UK
    Cell Calcium 33:385-93. 2003
  10. ncbi Regulation of TRP channels via lipid second messengers
    Roger C Hardie
    Department of Anatomy, Cambridge University, Downing St Cambridge CB2 3DY, United Kingdom
    Annu Rev Physiol 65:735-59. 2003

Collaborators

Detail Information

Publications53

  1. ncbi Vision: dynamic platforms
    Roger C Hardie
    Nature 450:37-9. 2007
  2. ncbi Functional equivalence of native light-sensitive channels in the Drosophila trp301 mutant and TRPL cation channels expressed in a stably transfected Drosophila cell line
    R C Hardie
    Department of Anatomy, Cambridge University, UK
    Cell Calcium 21:431-40. 1997
    ..These findings indicate that channels encoded by the trpl gene can completely account for the component of the light-sensitive conductance remaining in the trp mutant...
  3. ncbi Activation of heterologously expressed Drosophila TRPL channels: Ca2+ is not required and InsP3 is not sufficient
    R C Hardie
    Department of Anatomy, Cambridge University, UK
    Cell Calcium 24:153-63. 1998
    ..The results suggest that, as in situ, TRPL activation involves G-protein and PLC; that Ca2+ can both facilitate and in some cases inhibit TRPL channels, but that neither Ca2+ nor InsP3 is the primary activator of the channel...
  4. ncbi Phototransduction: shedding light on translocation
    Roger C Hardie
    Cambridge University Department of Anatomy, Downing Street, Cambridge CB2 3DY, UK
    Curr Biol 13:R775-7. 2003
    ..New work has identified a phosphoinositide lipid binding domain in Drosophila arrestin and implicates PIP(3) in control of arrestin translocation...
  5. ncbi In vivo light-induced and basal phospholipase C activity in Drosophila photoreceptors measured with genetically targeted phosphatidylinositol 4,5-bisphosphate-sensitive ion channels (Kir2.1)
    Roger C Hardie
    Department of Anatomy, Cambridge University, Downing St, Cambridge CB2 3DY, United Kingdom
    J Biol Chem 279:47773-82. 2004
    ....
  6. ncbi Calcium influx via TRP channels is required to maintain PIP2 levels in Drosophila photoreceptors
    R C Hardie
    Department of Anatomy, Cambridge, United Kingdom
    Neuron 30:149-59. 2001
    ..Without this feedback one photon appears sufficient to deplete the phosphoinositide pool of approximately 4 microvilli...
  7. ncbi Visual transduction in Drosophila
    R C Hardie
    Department of Anatomy, University of Cambridge, Downing Street, Cambridge CB2 3DY, UK
    Nature 413:186-93. 2001
    ..Analysis in Drosophila has revealed many of the underlying molecular strategies, leading to the discovery and characterization of signalling molecules of widespread importance...
  8. ncbi Phototransduction in Drosophila melanogaster
    R C Hardie
    Cambridge University, Department of Anatomy, Downing Street, Cambridge CB2 3DY, UK
    J Exp Biol 204:3403-9. 2001
    ....
  9. ncbi TRP channels in Drosophila photoreceptors: the lipid connection
    Roger C Hardie
    Department of Anatomy, Cambridge University, Downing Street, Cambridge CB2 3DY, UK
    Cell Calcium 33:385-93. 2003
    ..The rdgA mutation leads to constitutive activation of both TRP and TRPL channels and dramatically increases sensitivity to light in hypomorphic mutations of PLC and G protein...
  10. ncbi Regulation of TRP channels via lipid second messengers
    Roger C Hardie
    Department of Anatomy, Cambridge University, Downing St Cambridge CB2 3DY, United Kingdom
    Annu Rev Physiol 65:735-59. 2003
    ..Recent evidence indicates that a growing number of mammalian TRP homologues are also regulated by lipid messengers, including DAG, arachidonic acid, and PIP2...
  11. ncbi Mechanisms of light adaptation in Drosophila photoreceptors
    Yuchun Gu
    Department of Anatomy, Cambridge University, Downing Street, Cambridge CB2 3DY, United Kingdom
    Curr Biol 15:1228-34. 2005
    ....
  12. ncbi Rescue of light responses in the Drosophila "null" phospholipase C mutant, norpAP24, by the diacylglycerol kinase mutant, rdgA, and by metabolic inhibition
    Roger C Hardie
    Cambridge University Department of Anatomy, Cambridge CB2 3DY, United Kingdom
    J Biol Chem 278:18851-8. 2003
    ....
  13. ncbi Inhibition of phospholipase C activity in Drosophila photoreceptors by 1,2-bis(2-aminophenoxy)ethane N,N,N',N'-tetraacetic acid (BAPTA) and di-bromo BAPTA
    Roger C Hardie
    Cambridge University, Department of Anatomy, Downing St, Cambridge CB2 3DY, UK
    Cell Calcium 38:547-56. 2005
    ..The results indicate that both BAPTA and DBB inhibit PLC activity independently of their role as Ca2+ chelators, whilst non-physiologically low (<100 nM) levels of Ca2+ suppress both basal and light-induced PLC activity...
  14. ncbi TRP channels and lipids: from Drosophila to mammalian physiology
    Roger C Hardie
    Department of Physiology Development and Neuroscience, Cambridge University, Downing Street, Cambridge CB2 3DY, UK
    J Physiol 578:9-24. 2007
    ..Although compelling evidence for a direct interaction of DAG with the TRPC channels is lacking, mutagenesis studies have identified putative PIP2-interacting domains in the C-termini of several TRPV and TRPM channels...
  15. ncbi Molecular basis of amplification in Drosophila phototransduction: roles for G protein, phospholipase C, and diacylglycerol kinase
    R C Hardie
    Department of Anatomy, Cambridge University, Downing Street, Cambridge, United Kingdom
    Neuron 36:689-701. 2002
    ....
  16. ncbi Polyunsaturated fatty acids activate the Drosophila light-sensitive channels TRP and TRPL
    S Chyb
    Cambridge University Department of Anatomy, UK
    Nature 397:255-9. 1999
    ..As arachidonic acid may not be found in Drosophila, we suggest that another polyunsaturated fatty acid, such as linolenic acid, may be a messenger of excitation in Drosophila photoreceptors...
  17. ncbi Thapsigargin and receptor-mediated activation of Drosophila TRPL channels stably expressed in a Drosophila S2 cell line
    S Yagodin
    Babraham Institute Laboratory of Molecular Signalling, Department of Zoology, University of Cambridge, UK
    Cell Calcium 23:219-28. 1998
    ....
  18. ncbi In vivo analysis of the drosophila light-sensitive channels, TRP and TRPL
    H Reuss
    Department of Anatomy, Cambridge University, United Kingdom
    Neuron 19:1249-59. 1997
    ..The results indicate that the wild-type light-activated conductance is composed of two separate channels, with the properties of TRP- and TRPL-dependent channels as determined in the respective mutants...
  19. ncbi Constitutive activity of the light-sensitive channels TRP and TRPL in the Drosophila diacylglycerol kinase mutant, rdgA
    P Raghu
    Department of Anatomy, Cambridge University, United Kingdom
    Neuron 26:169-79. 2000
    ..The findings suggest that early degeneration in rdgA is caused by uncontrolled Ca2+ influx and support the proposal that diacylglycerol or its metabolites are messengers of excitation in Drosophila photoreceptors...
  20. ncbi Protein kinase C is required for light adaptation in Drosophila photoreceptors
    R C Hardie
    Department of Zoology, Cambridge University, UK
    Nature 363:634-7. 1993
    ..Furthermore, we show that in the absence of PKC individual bumps fail to terminate normally, an effect that can account for the pleiotropic manifestations of the inaC phenotype...
  21. ncbi Excitation of Drosophila photoreceptors by BAPTA and ionomycin: evidence for capacitative Ca2+ entry?
    R C Hardie
    Department of Anatomy, University of Cambridge, UK
    Cell Calcium 20:315-27. 1996
    ..However, activation by Ca-BAPTA and ionomycin had an absolute requirement for cytosolic Ca2+ as no currents could be activated by ionomycin in cells loaded with BAPTA and no Ca2+...
  22. ncbi Single photon responses in Drosophila photoreceptors and their regulation by Ca2+
    S R Henderson
    Cambridge University, Department of Anatomy, Downing Street, Cambridge CB2 3DY, UK
    J Physiol 524:179-94. 2000
    ..In InaDP215 mutants, in which the association of the TRP channels with the INAD scaffolding molecule is disrupted, bumps showed a defect in quantum bump termination, but their amplitudes and latencies were near normal...
  23. ncbi Modulation of the light response by cAMP in Drosophila photoreceptors
    S Chyb
    Department of Anatomy, Cambridge University, Cambridge CB2 3DY, United Kingdom
    J Neurosci 19:8799-807. 1999
    ..These results indicate the existence of a G-protein-coupled adenylyl cyclase pathway in Drosophila photoreceptors, which modulates the phospholipase C-based phototransduction cascade...
  24. ncbi Photolysis of caged Ca2+ facilitates and inactivates but does not directly excite light-sensitive channels in Drosophila photoreceptors
    R C Hardie
    Cambridge University, Department of Zoology, United Kingdom
    J Neurosci 15:889-902. 1995
    ..In conclusion, caged Ca2+ failed to activate any channels in Drosophila photoreceptors but profoundly affected the light-dependent channels once they have been activated...
  25. ncbi Novel Ca2+ channels underlying transduction in Drosophila photoreceptors: implications for phosphoinositide-mediated Ca2+ mobilization
    R C Hardie
    Dept of Zoology, University of Cambridge, UK
    Trends Neurosci 16:371-6. 1993
    ....
  26. ncbi Phosphoinositide-mediated phototransduction in Drosophila photoreceptors: the role of Ca2+ and trp
    R C Hardie
    Department of Anatomy, University of Cambridge, UK
    Cell Calcium 18:256-74. 1995
    ....
  27. ncbi Normal phototransduction in Drosophila photoreceptors lacking an InsP(3) receptor gene
    P Raghu
    Department of Anatomy, Cambridge University, United Kingdom
    Mol Cell Neurosci 15:429-45. 2000
    ..These results indicate that InsP(3)R-mediated store depletion does not underlie TRP and TRPL activation in Drosophila photoreceptors...
  28. ncbi Calcium-dependent inactivation of light-sensitive channels in Drosophila photoreceptors
    R C Hardie
    Department of Zoology, Cambridge University, United Kingdom
    J Gen Physiol 103:409-27. 1994
    ....
  29. ncbi Calcium signalling: setting store by calcium channels
    R C Hardie
    Cambridge University, Department of Anatomy, UK
    Curr Biol 6:1371-3. 1996
    ..Recent work, stemming from genetic studies of phototransduction in Drosophila, has identified a set of homologous channel proteins that appear to mediate capacitative Ca2+ entry in species from worms and files to humans...
  30. ncbi Magnesium-dependent block of the light-activated and trp-dependent conductance in Drosophila photoreceptors
    R C Hardie
    Department of Zoology, Cambridge University, United Kingdom
    J Neurophysiol 74:2590-9. 1995
    ..The concentration and voltage dependence of the Mg2+ block suggest it plays an important physiological role in determining the gain, kinetics, and signal-to-noise of transduction...
  31. ncbi Regulation of Drosophila TRP channels by lipid messengers
    Roger C Hardie
    Cambridge University Department of Anatomy, Downing Street, Cambridge CB2 3DY, UK
    Novartis Found Symp 258:160-7; discussion 167-71, 263-6. 2004
    ..Together with evidence from several mammalian TRP homologues, these results suggest that regulation by lipids may be a defining feature of many TRP channels...
  32. ncbi Effects of intracellular Ca2+ chelation on the light response in Drosophila photoreceptors
    R C Hardie
    Cambridge University, Department of Zoology, UK
    J Comp Physiol A 177:707-21. 1995
    ..However, its properties did not resemble those the light-activated conductance, and thus do not support the hypothesis that Ca2+ is sufficient for excitation...
  33. ncbi INDO-1 measurements of absolute resting and light-induced Ca2+ concentration in Drosophila photoreceptors
    R C Hardie
    Cambridge University, Department of Anatomy, United Kingdom
    J Neurosci 16:2924-33. 1996
    ..It is concluded that any light-induced release from internal stores amounts to <20 nM...
  34. ncbi Light adaptation in Drosophila photoreceptors: II. Rising temperature increases the bandwidth of reliable signaling
    M Juusola
    Physiological Laboratory, University of Cambridge, Cambridge CB2 3EG, UK
    J Gen Physiol 117:27-42. 2001
    ..Because faster neural processing allows faster behavioral responses, this improved performance of Drosophila photoreceptors suggests that a suitably high body temperature offers significant advantages in visual performance...
  35. ncbi Spontaneous activation of light-sensitive channels in Drosophila photoreceptors
    R C Hardie
    Department of Zoology, Cambridge University, United Kingdom
    J Gen Physiol 103:389-407. 1994
    ..2 ms). This implies that the noise in the WT RDC may actually be dominated by non-trp-dependent channels and that the trp-dependent channels may be of even lower unit conductance...
  36. ncbi Functional INAD complexes are required to mediate degeneration in photoreceptors of the Drosophila rdgA mutant
    Plamen Georgiev
    The Inositide Laboratory, Babraham Institute, Babraham Research Campus, Cambridge, CB2 4AT, UK
    J Cell Sci 118:1373-84. 2005
    ..They also suggest a key role for the lipid products of phosphoinositide hydrolysis in the activation of TRP channels in vivo...
  37. ncbi Light adaptation in Drosophila photoreceptors: I. Response dynamics and signaling efficiency at 25 degrees C
    M Juusola
    Physiological Laboratory, University of Cambridge, Cambridge CB2 3EG, United Kingdom
    J Gen Physiol 117:3-25. 2001
    ....
  38. ncbi The contribution of Shaker K+ channels to the information capacity of Drosophila photoreceptors
    Jeremy E Niven
    Physiological Laboratory, University of Cambridge, Cambridge CB2 3EG, UK
    Nature 421:630-4. 2003
    ....
  39. ncbi Ca2+-dependent metarhodopsin inactivation mediated by calmodulin and NINAC myosin III
    Che Hsiung Liu
    Department of Physiology, Development and Neuroscience, Cambridge University, Cambridge CB23DY, UK
    Neuron 59:778-89. 2008
    ..Our results indicate that this strategy promotes quantum efficiency, temporal resolution, and fidelity of visual signaling...
  40. ncbi Lipids in Ca2+ signalling--an introduction
    Roger C Hardie
    Department of Physiology Development and Neuroscience, Cambridge University, Downing St, Cambridge, United Kingdom
    Cell Calcium 45:517-20. 2009
    ..Although appreciated individually, it seems timely to highlight the overall impact of lipids as signalling molecules and their role in Ca(2+) signalling, and this is the aim of this special issue of Cell Calcium...
  41. ncbi In vivo identification and manipulation of the Ca2+ selectivity filter in the Drosophila transient receptor potential channel
    Che H Liu
    Department of Physiology Development and Neuroscience, Cambridge University, Cambridge CB2 3DY, United Kingdom
    J Neurosci 27:604-15. 2007
    ..We found that Asp621 mutations profoundly affected several key aspects of the light response and caused light-dependent retinal degeneration...
  42. ncbi A brief history of trp: commentary and personal perspective
    Roger C Hardie
    Department of Physiology, Development and Neuroscience, Cambridge University, Downing Street, Cambridge, CB2 3DY, UK
    Pflugers Arch 461:493-8. 2011
    ..Three years later, in 1995, the labs of Montell and Birnbaumer independently cloned TRPC1, the first of 29 vertebrate TRP isoforms distributed amongst seven subfamilies...
  43. ncbi Voltage-sensitive potassium channels in Drosophila photoreceptors
    R C Hardie
    Cambridge University, Department of Zoology, United Kingdom
    J Neurosci 11:3079-95. 1991
    ..Both IKs and IKf are present at the earliest pupal stages examined (approximately 60 hr), but IA was only first detected at approximately 76 hr.(ABSTRACT TRUNCATED AT 250 WORDS)..
  44. ncbi Regulation of Drosophila TRPC channels by lipid messengers
    Padinjat Raghu
    Inositide Laboratory, Babraham Institute, Cambridge, United Kingdom
    Cell Calcium 45:566-73. 2009
    ..Evidence on the role of these lipid elements in regulating TRP and TRPL activity is discussed in this review...
  45. ncbi Activation of TRP channels by protons and phosphoinositide depletion in Drosophila photoreceptors
    Jiehong Huang
    Department of Physiology, Development, and Neuroscience, Cambridge University, Cambridge CB2 3DY, UK
    Curr Biol 20:189-97. 2010
    ..The fact that hydrolysis of the phosphodiester bond in PIP(2) by PLC also releases a proton is seldom recognized and has neither been measured in vivo nor implicated previously in a signaling context...
  46. ncbi Distinct roles for two histamine receptors (hclA and hclB) at the Drosophila photoreceptor synapse
    Antonios Pantazis
    Department of Physiology, Development, and Neuroscience, University of Cambridge, Cambridge CB2 3DY, United Kingdom
    J Neurosci 28:7250-9. 2008
    ..Our results suggest that the native receptor at the LMC synapse is an HCLA homomer, whereas HCLB signaling via the lamina glia plays a previously unrecognized role in shaping the LMC postsynaptic response...
  47. ncbi Light-dependent modulation of Shab channels via phosphoinositide depletion in Drosophila photoreceptors
    Yani Krause
    University of Oulu, Department of Physical Sciences, Division of Biophysics, 90014 Oulun yliopisto, Finland
    Neuron 59:596-607. 2008
    ..The results indicate a functionally implemented mechanism of Kv channel modulation by PIP(2) in photoreceptors, which enables light-dependent regulation of signal processing by direct coupling to the phototransduction cascade...
  48. ncbi Feedback network controls photoreceptor output at the layer of first visual synapses in Drosophila
    Lei Zheng
    Department of Biomedical Science, University of Sheffield, UK
    J Gen Physiol 127:495-510. 2006
    ..These results highlight the benefits of feedback synapses for neural information processing, and suggest that similar coding strategies could be used in other nervous systems...
  49. ncbi Robustness of neural coding in Drosophila photoreceptors in the absence of slow delayed rectifier K+ channels
    Mikko Vähäsöyrinki
    Division of Biophysics and Biocenter Oulu, Department of Physical Sciences, University of Oulu, 90014 Oulun yliopisto, Finland
    J Neurosci 26:2652-60. 2006
    ..Our results highlight the role of different types of voltage-gated K+ channels in the performance of the photoreceptors and provide insight into functional robustness against the perturbation of specific ion channel composition...
  50. ncbi Transient receptor potential-like channels are essential for calcium signaling and fluid transport in a Drosophila epithelium
    Matthew R MacPherson
    IBLS Division of Molecular Genetics, University of Glasgow, UK
    Genetics 169:1541-52. 2005
    ..Finally, a scaffolding component of the TRPL/TRP-signaling complex, INAD, is not expressed in tubules, suggesting that inaD is not essential for TRPL/TRP function in Drosophila tubules...
  51. ncbi Light activation, adaptation, and cell survival functions of the Na+/Ca2+ exchanger CalX
    Tao Wang
    Department of Biological Chemistry, The Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
    Neuron 45:367-78. 2005
    ..These results illustrate the critical role of Ca(2+) for proper signaling and provide genetic evidence that Ca(2+) overload is responsible for a form of retinal degeneration resulting from defects in the TRP channel...
  52. ncbi The target of Drosophila photoreceptor synaptic transmission is a histamine-gated chloride channel encoded by ort (hclA)
    Chaoxian Gengs
    Department of Biological Sciences, Purdue University, West Lafayette, Indiana 47907, USA
    J Biol Chem 277:42113-20. 2002
    ..Moreover, the present work shows that this function of the HCLA (ORT) protein is its native function in vivo...
  53. ncbi Calnexin is essential for rhodopsin maturation, Ca2+ regulation, and photoreceptor cell survival
    Erica E Rosenbaum
    Department of Ophthalmology and Visual Sciences, Department of Genetics, University of Wisconsin, Madison, Wisconsin 53792, USA
    Neuron 49:229-41. 2006
    ..Our results illustrate a critical role for calnexin in Rh1 maturation and Ca2+ regulation and provide genetic evidence that defects in calnexin lead to retinal degeneration...