A P Halestrap

Summary

Affiliation: University of Bristol
Country: UK

Publications

  1. pmc The role of charged residues in the transmembrane helices of monocarboxylate transporter 1 and its ancillary protein basigin in determining plasma membrane expression and catalytic activity
    Christine Manoharan
    Department of Biochemistry, University of Bristol, School of Medical Sciences, University Walk, Bristol, UK
    Mol Membr Biol 23:486-98. 2006
  2. pmc The inhibition of monocarboxylate transporter 2 (MCT2) by AR-C155858 is modulated by the associated ancillary protein
    Matthew J Ovens
    Department of Biochemistry, School of Medical Sciences, University of Bristol, University Walk, Bristol, UK
    Biochem J 431:217-25. 2010
  3. pmc AR-C155858 is a potent inhibitor of monocarboxylate transporters MCT1 and MCT2 that binds to an intracellular site involving transmembrane helices 7-10
    Matthew J Ovens
    Department of Biochemistry, School of Medical Sciences, University of Bristol, University Walk, Bristol BS8 1TD, UK
    Biochem J 425:523-30. 2010
  4. pmc Studies on the DIDS-binding site of monocarboxylate transporter 1 suggest a homology model of the open conformation and a plausible translocation cycle
    Marieangela C Wilson
    Department of Biochemistry, University of Bristol, School of Medical Sciences, Bristol BS8 1TD, United Kingdom
    J Biol Chem 284:20011-21. 2009
  5. pmc Extent of mitochondrial hexokinase II dissociation during ischemia correlates with mitochondrial cytochrome c release, reactive oxygen species production, and infarct size on reperfusion
    Philippe Pasdois
    School of Biochemistry and The Bristol Heart Institute, University of Bristol, Bristol, UK
    J Am Heart Assoc 2:e005645. 2013
  6. pmc The role of oxidized cytochrome c in regulating mitochondrial reactive oxygen species production and its perturbation in ischaemia
    Philippe Pasdois
    School of Biochemistry and The Bristol Heart Institute, University of Bristol, Bristol BS8 1TD, UK
    Biochem J 436:493-505. 2011
  7. doi The SLC16 gene family - structure, role and regulation in health and disease
    Andrew P Halestrap
    School of Biochemistry, Medical Sciences Building, University of Bristol, Bristol BS8 1TD, UK
    Mol Aspects Med 34:337-49. 2013
  8. doi The mitochondrial pyruvate carrier: has it been unearthed at last?
    Andrew P Halestrap
    School of Biochemistry, Medical Sciences Building, University Walk, Bristol BS8 1TD, UK
    Cell Metab 16:141-3. 2012
  9. pmc The roles of phosphate and the phosphate carrier in the mitochondrial permeability transition pore
    Pinadda Varanyuwatana
    School of Biochemistry and The Bristol Heart Institute, Medical Sciences Building, University of Bristol, Bristol BS8 1TD, UK
    Mitochondrion 12:120-5. 2012
  10. doi What is the mitochondrial permeability transition pore?
    Andrew P Halestrap
    Department of Biochemistry and Bristol Heart Institute, University of Bristol, School of Medical Sciences, University Walk, Bristol, UK
    J Mol Cell Cardiol 46:821-31. 2009

Collaborators

Detail Information

Publications65

  1. pmc The role of charged residues in the transmembrane helices of monocarboxylate transporter 1 and its ancillary protein basigin in determining plasma membrane expression and catalytic activity
    Christine Manoharan
    Department of Biochemistry, University of Bristol, School of Medical Sciences, University Walk, Bristol, UK
    Mol Membr Biol 23:486-98. 2006
    ....
  2. pmc The inhibition of monocarboxylate transporter 2 (MCT2) by AR-C155858 is modulated by the associated ancillary protein
    Matthew J Ovens
    Department of Biochemistry, School of Medical Sciences, University of Bristol, University Walk, Bristol, UK
    Biochem J 431:217-25. 2010
    ..The C-terminus of MCT2 was found to be essential for its expression with endogenous basigin...
  3. pmc AR-C155858 is a potent inhibitor of monocarboxylate transporters MCT1 and MCT2 that binds to an intracellular site involving transmembrane helices 7-10
    Matthew J Ovens
    Department of Biochemistry, School of Medical Sciences, University of Bristol, University Walk, Bristol BS8 1TD, UK
    Biochem J 425:523-30. 2010
    ..Biol. Chem. 284, 20011-20021]...
  4. pmc Studies on the DIDS-binding site of monocarboxylate transporter 1 suggest a homology model of the open conformation and a plausible translocation cycle
    Marieangela C Wilson
    Department of Biochemistry, University of Bristol, School of Medical Sciences, Bristol BS8 1TD, United Kingdom
    J Biol Chem 284:20011-21. 2009
    ..The cross-linking data have also been used to model a structure of MCT1 bound to embigin that is consistent with published data...
  5. pmc Extent of mitochondrial hexokinase II dissociation during ischemia correlates with mitochondrial cytochrome c release, reactive oxygen species production, and infarct size on reperfusion
    Philippe Pasdois
    School of Biochemistry and The Bristol Heart Institute, University of Bristol, Bristol, UK
    J Am Heart Assoc 2:e005645. 2013
    ..Here we investigate whether and how mitochondria-bound hexokinase 2 (mtHK2) may exert part of the cardioprotective effects of IP...
  6. pmc The role of oxidized cytochrome c in regulating mitochondrial reactive oxygen species production and its perturbation in ischaemia
    Philippe Pasdois
    School of Biochemistry and The Bristol Heart Institute, University of Bristol, Bristol BS8 1TD, UK
    Biochem J 436:493-505. 2011
    ..We further suggest that in hypoxia, production of H₂O₂ to activate signalling pathways may be also mediated by decreased oxidized cytochrome c and less superoxide scavenging...
  7. doi The SLC16 gene family - structure, role and regulation in health and disease
    Andrew P Halestrap
    School of Biochemistry, Medical Sciences Building, University of Bristol, Bristol BS8 1TD, UK
    Mol Aspects Med 34:337-49. 2013
    ..It is suggested that the development of other drugs specifically targeting different MCT isoforms may provide a novel approach to cancer chemotherapy...
  8. doi The mitochondrial pyruvate carrier: has it been unearthed at last?
    Andrew P Halestrap
    School of Biochemistry, Medical Sciences Building, University Walk, Bristol BS8 1TD, UK
    Cell Metab 16:141-3. 2012
    ..Two recent papers in Science (Herzig et al., 2012; Bricker et al., 2012) implicate three newly identified inner mitochondrial membrane proteins as MPC components...
  9. pmc The roles of phosphate and the phosphate carrier in the mitochondrial permeability transition pore
    Pinadda Varanyuwatana
    School of Biochemistry and The Bristol Heart Institute, Medical Sciences Building, University of Bristol, Bristol BS8 1TD, UK
    Mitochondrion 12:120-5. 2012
    ..Using siRNA in HeLa cells we could reduce PiC expression by 65-80% but this inhibited neither mitochondrial calcium accumulation nor MPTP opening...
  10. doi What is the mitochondrial permeability transition pore?
    Andrew P Halestrap
    Department of Biochemistry and Bristol Heart Institute, University of Bristol, School of Medical Sciences, University Walk, Bristol, UK
    J Mol Cell Cardiol 46:821-31. 2009
    ..Agents that modulate pore opening may act on either or both the PiC and the ANT. However, knockdown and reconstitution studies are awaited to confirm or refute this model...
  11. pmc The role of mitochondria in protection of the heart by preconditioning
    Andrew P Halestrap
    Department of Biochemistry and Bristol Heart Institute, University of Bristol, School of Medical Sciences, University Walk, Bristol BS8 1TD, UK
    Biochim Biophys Acta 1767:1007-31. 2007
    ..This phase of protection may involve survival kinase pathways such as Akt and glycogen synthase kinase 3 (GSK3) either increasing ROS removal or reducing mitochondrial ROS production...
  12. ncbi The SLC16 gene family-from monocarboxylate transporters (MCTs) to aromatic amino acid transporters and beyond
    Andrew P Halestrap
    Department of Biochemistry, University of Bristol, BS8 1TD, Bristol, UK
    Pflugers Arch 447:619-28. 2004
    ..The properties of the different MCT isoforms and their tissue distribution and regulation reflect these roles...
  13. ncbi The adenine nucleotide translocase: a central component of the mitochondrial permeability transition pore and key player in cell death
    Andrew P Halestrap
    Department of Biochemistry, School of Medical Sciences, University of Bristol, Bristol BS8 1TD, UK
    Curr Med Chem 10:1507-25. 2003
    ..Thus the ANT may provide a molecular link between physiopathological mechanisms of infection and the regulation of MPTP function and so represents a potential therapeutic target...
  14. ncbi Mitochondrial permeability: dual role for the ADP/ATP translocator?
    Andrew P Halestrap
    Department of Biochemistry, University of Bristol, Bristol BS8 1TD, UK
    Nature 430:1 p following 983. 2004
    ..These results, which contradict previous evidence and cast doubt on a widely accepted model for the mtPTP (ref. 1), warrant scrutiny and call for a fundamental reappraisal of the role of the ANT in liver metabolism...
  15. ncbi Calcium, mitochondria and reperfusion injury: a pore way to die
    A P Halestrap
    Department of Biochemistry, University of Bristol, School of Medical Sciences, University Walk, Bristol BS8 1TD, UK
    Biochem Soc Trans 34:232-7. 2006
    ..However, the CyP-D-knockout mice develop normally and show no protection against a range of apoptotic stimuli, suggesting that the MPTP does not play a role in most forms of apoptosis...
  16. doi The role of the mitochondrial permeability transition pore in heart disease
    Andrew P Halestrap
    Department of Biochemistry and Bristol Heart Institute, University of Bristol, School of Medical Sciences, University Walk, Bristol BS8 1TD, UK
    Biochim Biophys Acta 1787:1402-15. 2009
    ..This confirms the mPTP as a promising drug target in human cardiovascular disease. Indeed, the first clinical trials have shown CsA treatment improves recovery after treatment of a coronary thrombosis with angioplasty...
  17. doi Mitochondria and reperfusion injury of the heart--a holey death but not beyond salvation
    Andrew P Halestrap
    The Department of Biochemistry and The Bristol Heart Institute, School of Medical Sciences, University of Bristol, University Walk, Bristol, BS8 1TD, UK
    J Bioenerg Biomembr 41:113-21. 2009
    ..Our own studies have implicated a calcium-triggered conformational change of the mitochondrial phosphate carrier that is facilitated by CyP-D and modulated by the conformation of the ANT...
  18. doi The monocarboxylate transporter family--Structure and functional characterization
    Andrew P Halestrap
    School of Biochemistry, Medical Sciences Building, University of Bristol, Bristol, UK
    IUBMB Life 64:1-9. 2012
    ..MCT1, MCT3, and MCT4 bind preferentially to basigin and MCT2 to embigin. The choice of binding partner does not affect substrate specificity or kinetics but can influence inhibitor specificity...
  19. pmc The proton-linked monocarboxylate transporter (MCT) family: structure, function and regulation
    A P Halestrap
    Department of Biochemistry, School of Medical Sciences, University of Bristol, Bristol BS8 1TD, U K
    Biochem J 343:281-99. 1999
    ..In the future it will be interesting to explore the linkage of genetic diseases to particular MCTs through their chromosomal location...
  20. doi The monocarboxylate transporter family--role and regulation
    Andrew P Halestrap
    School of Biochemistry, Medical Sciences Building, University of Bristol, Bristol, UK
    IUBMB Life 64:109-19. 2012
    ..The recent discovery of potent and specific MCT1 inhibitors that prevent proliferation of T-lymphocytes confirms that MCTs may be promising pharmacological targets including for cancer chemotherapy...
  21. ncbi Mitochondrial permeability transition pore opening during myocardial reperfusion--a target for cardioprotection
    Andrew P Halestrap
    Department of Biochemistry and The Bristol Heart Institute, University of Bristol, Bristol BS8 1TD, UK
    Cardiovasc Res 61:372-85. 2004
    ..However, only if subsequent MPTP closure occurs will ATP levels be maintained, ensuring that cell death continues down an apoptotic, rather than a necrotic, pathway...
  22. ncbi Oxidative stress, thiol reagents, and membrane potential modulate the mitochondrial permeability transition by affecting nucleotide binding to the adenine nucleotide translocase
    A P Halestrap
    Department of Biochemistry, School of Medical Sciences, University of Bristol, Bristol BS8 1TD, United Kingdom
    J Biol Chem 272:3346-54. 1997
    ..Both mechanisms act to decrease the Ca2+ sensitivity of the pore. Thiol reagents and oxidative stress may modify two thiol groups on the ANT and thus stimulate pore opening by both means...
  23. ncbi The permeability transition pore complex: another view
    Andrew P Halestrap
    Department of Biochemistry, University of Bristol, Bristol BS8 1TD, UK
    Biochimie 84:153-66. 2002
    ..Adenine nucleotide binding to the ANT is inhibited by Cys(159) modification whilst oxidation of Cys(56) increases CyP-D binding to the ANT, probably at Pro(61)...
  24. pmc Cloning of the monocarboxylate transporter isoform MCT2 from rat testis provides evidence that expression in tissues is species-specific and may involve post-transcriptional regulation
    V N Jackson
    Department of Biochemistry, School of Medical Sciences, University of Bristol, Bristol BS8 1TD, U K
    Biochem J 324:447-53. 1997
    ..The different-sized MCT2 transcripts may arise from alternative splicing. Starvation of rats for up to 48 h did not lead to any change in MCT1 or MCT2 expression in the liver, as determined by either Northern or Western blotting...
  25. pmc Cloning and sequencing of four new mammalian monocarboxylate transporter (MCT) homologues confirms the existence of a transporter family with an ancient past
    N T Price
    Department of Biochemistry, School of Medical Sciences, University of Bristol, Bristol BS8 1TD, U K
    Biochem J 329:321-8. 1998
    ..Together these sequences constitute a new family of transporters with some strongly conserved sequence motifs, the possible functions of which are discussed...
  26. ncbi Lactic acid efflux from white skeletal muscle is catalyzed by the monocarboxylate transporter isoform MCT3
    M C Wilson
    Department of Biochemistry, School of Medical Sciences, University of Bristol, Bristol BS8 1TD, United Kingdom
    J Biol Chem 273:15920-6. 1998
    ..Differences in the properties of MCT1 and MCT3 are relatively modest, suggesting that the significance of the two isoforms may be related to their regulation rather than their intrinsic properties...
  27. pmc Characterisation of human monocarboxylate transporter 4 substantiates its role in lactic acid efflux from skeletal muscle
    J E Manning Fox
    Department of Biochemistry, School of Medical Sciences, University of Bristol, Bristol BS8 1TD, UK
    J Physiol 529:285-93. 2000
    ....
  28. ncbi Elucidating the molecular mechanism of the permeability transition pore and its role in reperfusion injury of the heart
    A P Halestrap
    Department of Biochemistry, University of Bristol, Bristol BS8 1TD
    Biochim Biophys Acta 1366:79-94. 1998
    ..Third we discuss how the MPT may be involved in determining whether cell death occurs by necrosis (extensive pore opening and ATP depletion) or apoptosis (transient pore opening with maintenance of ATP)...
  29. pmc Direct demonstration of a specific interaction between cyclophilin-D and the adenine nucleotide translocase confirms their role in the mitochondrial permeability transition
    K Woodfield
    Department of Biochemistry, School of Medical Sciences, University of Bristol, Bristol BS8 1TD, U K
    Biochem J 336:287-90. 1998
    ..Binding was prevented by pretreatment of the CyP-D with CsA, but not with cyclosporin H. Purified ANT also bound specifically to GST-CyP-D, but porin did not, even in the presence of ANT...
  30. ncbi Recent progress in elucidating the molecular mechanism of the mitochondrial permeability transition pore
    Anna W C Leung
    Department of Biochemistry and Bristol Heart Institute, University of Bristol, School of Medical Sciences, University Walk, Bristol BS8 1TD, UK
    Biochim Biophys Acta 1777:946-52. 2008
    ..We propose that this is enhanced by an association of the PiC with the "c" conformation of the ANT. Agents that modulate pore opening may act on either or both the PiC and the ANT...
  31. ncbi The mechanisms by which mild respiratory chain inhibitors inhibit hepatic gluconeogenesis
    M R Owen
    Department of Biochemistry, School of Medical Sciences, University of Bristol, UK
    Biochim Biophys Acta 1142:11-22. 1993
    ..1973) J. Biol. Chem. 248, 5272-5277) are similar to those caused by DCMU, supporting a mitochondrial locus of action for this hypoglycaemic agent...
  32. pmc Temperature preconditioning of isolated rat hearts--a potent cardioprotective mechanism involving a reduction in oxidative stress and inhibition of the mitochondrial permeability transition pore
    Igor Khaliulin
    Department of Biochemistry, Bristol Heart Institute, University of Bristol, UK
    J Physiol 581:1147-61. 2007
    ..The presence of N-(2-mercaptopropionyl) glycine during TP also abolished cardioprotection, indicating an involvement of free radicals in the signalling mechanism...
  33. ncbi Mitochondria and cell death
    A P Halestrap
    Department of Biochemistry, University of Bristol, UK
    Biochem Soc Trans 28:170-7. 2000
    ..Our own data suggest that this pathway may interact directly with the ANT in the inner membrane at contact sites...
  34. ncbi Basigin (CD147) is the target for organomercurial inhibition of monocarboxylate transporter isoforms 1 and 4: the ancillary protein for the insensitive MCT2 is EMBIGIN (gp70)
    Marieangela C Wilson
    Department of Biochemistry, University of Bristol, School of Medical Sciences, University Walk, Bristol BS8 1TD, United Kingdom
    J Biol Chem 280:27213-21. 2005
    ..We conclude that ancillary proteins are required to maintain the catalytic activity of MCTs as well as for their translocation to the plasma membrane...
  35. pmc The mitochondrial phosphate carrier interacts with cyclophilin D and may play a key role in the permeability transition
    Anna W C Leung
    Department of Biochemistry and The Bristol Heart Institute, University of Bristol, School of Medical Sciences, University Walk, Bristol BS8 1TD, United Kingdom
    J Biol Chem 283:26312-23. 2008
    ..An interaction of the PiC with the ANT may enable agents that bind to either transporter to modulate pore opening...
  36. ncbi cDNA cloning of MCT1, a monocarboxylate transporter from rat skeletal muscle
    V N Jackson
    Department of Biochemistry, University of Bristol, UK
    Biochim Biophys Acta 1238:193-6. 1995
    ..The cDNA derived amino acid sequence shows 94% and 86% identity to CHO and human MCT1, respectively...
  37. ncbi Cloning and sequencing of the monocarboxylate transporter from mouse Ehrlich Lettré tumour cell confirms its identity as MCT1 and demonstrates that glycosylation is not required for MCT1 function
    L Carpenter
    Department of Biochemistry, School of Medical Sciences, University of Bristol, UK
    Biochim Biophys Acta 1279:157-63. 1996
    ..These results are discussed in relation to current understanding of the roles of glycosylation of membrane proteins...
  38. ncbi Mitochondria: a target for myocardial protection
    M S Suleiman
    Bristol Heart Institute, University of Bristol, Bristol Royal Infirmary, Malborough Street, Bristol BS2 8HW, UK
    Pharmacol Ther 89:29-46. 2001
    ..Finally, we describe use of cardioplegic solutions in the clinical setting, and discuss how improved understanding of the aspects of mitochondrial function summarised above may lead to better protective strategies in the future...
  39. pmc Ischaemic preconditioning inhibits opening of mitochondrial permeability transition pores in the reperfused rat heart
    Sabzali A Javadov
    Department of Biochemistry, University of Bristol, Bristol BS8 1TD, UK
    J Physiol 549:513-24. 2003
    ..Our data suggest that protection from reperfusion injury is better achieved by reducing factors that induce MPTP opening than by inhibiting the MPTP directly...
  40. pmc Inhibition of mitochondrial permeability transition pore opening by ischemic preconditioning is probably mediated by reduction of oxidative stress rather than mitochondrial protein phosphorylation
    Samantha J Clarke
    Department of Biochemistry and The Bristol Heart Institute, University of Bristol, Bristol BS8 1TD, United Kingdom
    Circ Res 102:1082-90. 2008
    ..The signaling pathways mediating this effect and maintaining it during reperfusion are discussed...
  41. pmc Role of critical thiol groups on the matrix surface of the adenine nucleotide translocase in the mechanism of the mitochondrial permeability transition pore
    Gavin P McStay
    Department of Biochemistry, School of Medical Sciences, University of Bristol, Bristol BS8 1TD, U K
    Biochem J 367:541-8. 2002
    ..This cross-linking stabilizes the "c" conformation of the ANT, reducing the reactivity of Cys(57), while enhancing CyP-D binding to the ANT and antagonizing ADP binding. The two effects together greatly sensitize the MPTP to [Ca(2+)]...
  42. pmc Purification and N-terminal sequencing of peptidyl-prolyl cis-trans-isomerase from rat liver mitochondrial matrix reveals the existence of a distinct mitochondrial cyclophilin
    C P Connern
    Department of Biochemistry, School of Medical Sciences, University of Bristol, U K
    Biochem J 284:381-5. 1992
    ..6. Parallel purification and N-terminal sequencing of rat cytosolic PPIase showed the two proteins to have significant differences, implying that they are probably products of separate genes...
  43. pmc Consecutive pharmacological activation of PKA and PKC mimics the potent cardioprotection of temperature preconditioning
    Igor Khaliulin
    Department of Biochemistry and The Bristol Heart Institute, University of Bristol, University Walk, Bristol BS8 1TD, UK
    Cardiovasc Res 88:324-33. 2010
    ..We investigated the interrelationship between activation of protein kinase A (PKA) and protein kinase C (PKC) in the signalling mechanisms of TP and developed a potent pharmacological intervention based on this mechanism...
  44. ncbi Sanglifehrin A acts as a potent inhibitor of the mitochondrial permeability transition and reperfusion injury of the heart by binding to cyclophilin-D at a different site from cyclosporin A
    Samantha J Clarke
    Department of Biochemistry, School of Medical Sciences, University of Bristol, Bristol BS8 1TD, United Kingdom
    J Biol Chem 277:34793-9. 2002
    ..Because SfA does not inhibit calcineurin activity, our data suggest that it may be more desirable than CsA for protecting tissues recovering from ischemic episodes and for studying the role of the MPTP in cell death...
  45. ncbi A pore way to die: the role of mitochondria in reperfusion injury and cardioprotection
    Andrew P Halestrap
    Department of Biochemistry and The Bristol Heart Institute, University of Bristol, School of Medical Sciences, University Walk, Bristol BS8 1TD, UK
    Biochem Soc Trans 38:841-60. 2010
    ..MPTP inhibition also protects against congestive heart failure in hypertensive animal models. Thus the MPTP is a very promising pharmacological target for clinical practice, especially once more specific drugs are developed...
  46. ncbi Identification and characterisation of a new class of highly specific and potent inhibitors of the mitochondrial pyruvate carrier
    John C W Hildyard
    Department of Biochemistry, School of Medical Sciences, University of Bristol, Bristol BS8 1TD, UK
    Biochim Biophys Acta 1707:221-30. 2005
    ..GW604714X and GW450863X inhibited l-lactate transport by the plasma membrane monocarboxylate transporter MCT1, but at concentrations more than four orders of magnitude greater than the MPC...
  47. pmc Matrix volume measurements challenge the existence of diazoxide/glibencamide-sensitive KATP channels in rat mitochondria
    Manika Das
    Department of Biochemistry, University of Bristol, Bristol BS8 1TD, UK
    J Physiol 547:893-902. 2003
    ..2 nM), produced a 10-20 % increase in matrix volume that was readily detectable by both techniques. Our data argue against the existence of a sulphonylurea-inhibitable mitoKATP channel...
  48. pmc Tumour necrosis factor-alpha activation of protein kinase B in WEHI-164 cells is accompanied by increased phosphorylation of Ser473, but not Thr308
    S K Moule
    Department of Biochemistry, School of Medical Sciences, University of Bristol, Bristol BS8 1TD, UK
    Biochem J 359:119-27. 2001
    ..Possible explanations of these observations are discussed...
  49. ncbi Fluorescence resonance energy transfer studies on the interaction between the lactate transporter MCT1 and CD147 provide information on the topology and stoichiometry of the complex in situ
    Marieangela C Wilson
    Department of Biochemistry, School of Medical Sciences, University of Bristol, Bristol BS8 1TD, United Kingdom
    J Biol Chem 277:3666-72. 2002
    ..FRET may provide a non-invasive technique for measuring changes in these interactions in living cells...
  50. ncbi Intracellular ATP-sensitive K+ channels in mouse pancreatic beta cells: against a role in organelle cation homeostasis
    A Varadi
    Henry Wellcome Laboratories for Integrated Cell Signalling and Department of Biochemistry, School of Medical Sciences, University of Bristol, University Walk, Bristol, BS8 1TD, UK
    Diabetologia 49:1567-77. 2006
    ..The aim of this study was to explore these possibilities and to test the hypothesis that vesicle-resident channels play a role in the control of organellar Ca(2+) concentration or pH...
  51. ncbi The plasma membrane lactate transporter MCT4, but not MCT1, is up-regulated by hypoxia through a HIF-1alpha-dependent mechanism
    Mohammed S Ullah
    Department of Biochemistry, University of Bristol, School of Medical Sciences, University Walk, Bristol BS8 1TD, United Kingdom
    J Biol Chem 281:9030-7. 2006
    ..We conclude that MCT4, like other glycolytic enzymes, is up-regulated by hypoxia through a HIF-1alpha-mediated mechanism. This adaptive response allows the increased lactic acid produced during hypoxia to be rapidly lost from the cell...
  52. ncbi Glutamate loading protects freshly isolated and perfused adult cardiomyocytes against intracellular ROS generation
    Nicola King
    Bristol Heart Institute, University of Bristol, Bristol Royal Infirmary, Bristol BS2 8HW, UK
    J Mol Cell Cardiol 35:975-84. 2003
    ..These results provide new evidence to show that the cardioprotective effect of glutamate loading may be mediated through an enhanced ability to destroy ROS in the cell...
  53. ncbi Propofol is cardioprotective in a clinically relevant model of normothermic blood cardioplegic arrest and cardiopulmonary bypass
    Kelvin H H Lim
    Bristol Heart Institute, University of Bristol, Bristol Royal Infirmary, Bristol BS2 8HW, United Kingdom
    Exp Biol Med (Maywood) 230:413-20. 2005
    ..Propofol may therefore be a useful adjunct to cardioplegic solutions as well as being an appropriate anesthetic for cardiac surgery...
  54. pmc The effects of ischaemic preconditioning, diazoxide and 5-hydroxydecanoate on rat heart mitochondrial volume and respiration
    Kelvin H H Lim
    The Bristol Heart Institute, Bristol Royal Infirmary, Malborough Street, Bristol BS2 8HW, UK
    J Physiol 545:961-74. 2002
    ..These data highlight the dangers of using 5HD and diazoxide as specific modulators of mitoK(ATP) channels in the heart...
  55. doi Determination of the rate of K(+) movement through potassium channels in isolated rat heart and liver mitochondria
    Piotr Bednarczyk
    Department of Biophysics, Warsaw University Life of Science SGGW, Warsaw, Poland
    Biochim Biophys Acta 1777:540-8. 2008
    ....
  56. ncbi Does the mitochondrial permeability transition have a role in preconditioning?
    Andrew P Halestrap
    Circulation 110:e303; author reply e303. 2004
  57. ncbi Association between mutations in a thyroid hormone transporter and severe X-linked psychomotor retardation
    Edith C H Friesema
    Department of Internal Medicine, Erasmus MC, Rotterdam, Netherlands
    Lancet 364:1435-7. 2004
    ..We suggest that this novel syndrome of X-linked psychomotor retardation is due to a defect in T3 entry into neurons through MCT8, resulting in impaired T3 action and metabolism...
  58. ncbi Mitochondria and preconditioning: a connexin connection?
    Andrew P Halestrap
    Circ Res 99:10-2. 2006
  59. pmc Urocortin prevents mitochondrial permeability transition in response to reperfusion injury indirectly by reducing oxidative stress
    Paul A Townsend
    Human Genetics Division, University of Southampton, Southampton, UK
    Am J Physiol Heart Circ Physiol 293:H928-38. 2007
    ..Our data suggest that acute UCN treatment protects the heart by inhibiting MPTP opening. However, the mechanism appears to be indirect, involving a PKC-mediated reduction in oxidative stress...
  60. ncbi Mechanism of action of the antifibrogenic compound gliotoxin in rat liver cells
    James G Orr
    Department of Molecular and Cell Biology, Institute of Medical Sciences, University of Aberdeen, Aberdeen, UK
    Hepatology 40:232-42. 2004
    ..Further understanding of this mechanism of cell death will aid in finding therapeutics that specifically stimulate HSC apoptosis in the liver, a promising approach to antifibrotic therapy...
  61. ncbi Cellular and subcellular distribution of monocarboxylate transporters in cultured brain cells and in the adult brain
    Luc Pellerin
    Department of Physiology, University of Lausanne, 7 rue du Bugnon, 1005 Lausanne, Switzerland
    J Neurosci Res 79:55-64. 2005
    ....
  62. ncbi Identification of monocarboxylate transporter 8 as a specific thyroid hormone transporter
    Edith C H Friesema
    Department of Internal Medicine, Erasmus University Medical Center, Rotterdam 3015 GE, The Netherlands
    J Biol Chem 278:40128-35. 2003
    ..Immunohistochemistry showed high expression in liver, kidney, brain, and heart. In conclusion, we have identified MCT8 as a very active and specific thyroid hormone transporter...
  63. pmc Inhibition of human immunodeficiency virus type 1 replication in human cells by Debio-025, a novel cyclophilin binding agent
    Roger G Ptak
    Southern Research Institute, Frederick, Maryland 21701, USA
    Antimicrob Agents Chemother 52:1302-17. 2008
    ..Thus, Debio-025 seems to interfere with the function of CypA during the progression/completion of HIV-1 reverse transcription...
  64. pmc Physical exercise-induced hypoglycemia caused by failed silencing of monocarboxylate transporter 1 in pancreatic beta cells
    Timo Otonkoski
    Hospital for Children and Adolescents and Biomedicum Helsinki, University of Helsinki, Helsinki, Finland
    Am J Hum Genet 81:467-74. 2007
    ..These findings describe a novel disease mechanism based on the failure of cell-specific transcriptional silencing of a gene that is highly expressed in other tissues...
  65. pmc Enhanced expression of three monocarboxylate transporter isoforms in the brain of obese mice
    Karin Pierre
    Departement de Physiologie, Universite de Lausanne, Lausanne, Switzerland
    J Physiol 583:469-86. 2007
    ..This observation could be relevant in the context of obesity development and its consequences for brain function...