Keith Gull

Summary

Affiliation: University of Oxford
Country: UK

Publications

  1. pmc Evolution of tubulin gene arrays in Trypanosomatid parasites: genomic restructuring in Leishmania
    Andrew P Jackson
    Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridgeshire, CB10 1SA, UK
    BMC Genomics 7:261. 2006
  2. ncbi request reprint Host-parasite interactions and trypanosome morphogenesis: a flagellar pocketful of goodies
    Keith Gull
    Sir William Dunn School of Pathology, University of Oxford, South Parks Road, Oxford OX1 3RE, UK
    Curr Opin Microbiol 6:365-70. 2003
  3. pmc The expanded Kinesin-13 repertoire of trypanosomes contains only one mitotic Kinesin indicating multiple extra-nuclear roles
    Bill Wickstead
    Sir William Dunn School of Pathology, University of Oxford, Oxford, United Kingdom
    PLoS ONE 5:e15020. 2010
  4. doi request reprint Functional studies of an evolutionarily conserved, cytochrome b5 domain protein reveal a specific role in axonemal organisation and the general phenomenon of post-division axonemal growth in trypanosomes
    Helen Farr
    Sir William Dunn School of Pathology, University of Oxford, South Parks Road, Oxford, United Kingdom
    Cell Motil Cytoskeleton 66:24-35. 2009
  5. pmc Basal body movements orchestrate membrane organelle division and cell morphogenesis in Trypanosoma brucei
    Sylvain Lacomble
    Sir William Dunn School of Pathology, University of Oxford, South Parks Road, Oxford, OX1 3RE, UK
    J Cell Sci 123:2884-91. 2010
  6. pmc Combining RNA interference mutants and comparative proteomics to identify protein components and dependences in a eukaryotic flagellum
    Neil Portman
    Sir William Dunn School of Pathology and Oxford Centre for Integrative Systems Biology, University of Oxford, South Parks Road, Oxford OX1 3RE, United Kingdom
    J Biol Chem 284:5610-9. 2009
  7. ncbi request reprint Basal body and flagellum mutants reveal a rotational constraint of the central pair microtubules in the axonemes of trypanosomes
    Catarina Gadelha
    Sir William Dunn School of Pathology, University of Oxford, South Parks Road, Oxford, OX1 3RE, UK
    J Cell Sci 119:2405-13. 2006
  8. ncbi request reprint Cell morphogenesis of Trypanosoma brucei requires the paralogous, differentially expressed calpain-related proteins CAP5.5 and CAP5.5V
    Sofia Olego-Fernandez
    Sir William Dunn School of Pathology, University of Oxford, South Parks Road, Oxford OX1 3RE, UK
    Protist 160:576-90. 2009
  9. pmc Detailed interrogation of trypanosome cell biology via differential organelle staining and automated image analysis
    Richard J Wheeler
    The Sir William Dunn School of Pathology, University of Oxford, South Parks Road, Oxford, OX1 3RE, UK
    BMC Biol 10:1. 2012
  10. doi request reprint The evolution of land plant cilia
    Matthew E Hodges
    Department of Plant Sciences, South Parks Road, University of Oxford, Oxford OX1 3RB, UK
    New Phytol 195:526-40. 2012

Detail Information

Publications80

  1. pmc Evolution of tubulin gene arrays in Trypanosomatid parasites: genomic restructuring in Leishmania
    Andrew P Jackson
    Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridgeshire, CB10 1SA, UK
    BMC Genomics 7:261. 2006
    ..brucei. Polycistronic transcription in these organisms makes the chromosomal arrangement of tubulin genes important with respect to gene expression...
  2. ncbi request reprint Host-parasite interactions and trypanosome morphogenesis: a flagellar pocketful of goodies
    Keith Gull
    Sir William Dunn School of Pathology, University of Oxford, South Parks Road, Oxford OX1 3RE, UK
    Curr Opin Microbiol 6:365-70. 2003
    ..Recent work has highlighted the molecules and morphogenesis of these cytoskeletal/flagellum structures and how dynamic events, occurring in the flagellar pocket and kinetoplast, are critical for host-parasite interactions...
  3. pmc The expanded Kinesin-13 repertoire of trypanosomes contains only one mitotic Kinesin indicating multiple extra-nuclear roles
    Bill Wickstead
    Sir William Dunn School of Pathology, University of Oxford, Oxford, United Kingdom
    PLoS ONE 5:e15020. 2010
    ....
  4. doi request reprint Functional studies of an evolutionarily conserved, cytochrome b5 domain protein reveal a specific role in axonemal organisation and the general phenomenon of post-division axonemal growth in trypanosomes
    Helen Farr
    Sir William Dunn School of Pathology, University of Oxford, South Parks Road, Oxford, United Kingdom
    Cell Motil Cytoskeleton 66:24-35. 2009
    ..Our results provide evidence for TAX-2 function within the axoneme, where we suggest that it is involved in processes linking the outer doublet microtubules and the central pair...
  5. pmc Basal body movements orchestrate membrane organelle division and cell morphogenesis in Trypanosoma brucei
    Sylvain Lacomble
    Sir William Dunn School of Pathology, University of Oxford, South Parks Road, Oxford, OX1 3RE, UK
    J Cell Sci 123:2884-91. 2010
    ..This rotation and subsequent posterior movements results in division of the flagellar pocket and ultimately sets parameters for subsequent daughter cell morphogenesis...
  6. pmc Combining RNA interference mutants and comparative proteomics to identify protein components and dependences in a eukaryotic flagellum
    Neil Portman
    Sir William Dunn School of Pathology and Oxford Centre for Integrative Systems Biology, University of Oxford, South Parks Road, Oxford OX1 3RE, United Kingdom
    J Biol Chem 284:5610-9. 2009
    ..We extended the RNA interference mutant/comparative proteomic analysis to individual novel components of our PFR proteome, showing that the approach has the power to reveal dependences between subgroups within the cohort...
  7. ncbi request reprint Basal body and flagellum mutants reveal a rotational constraint of the central pair microtubules in the axonemes of trypanosomes
    Catarina Gadelha
    Sir William Dunn School of Pathology, University of Oxford, South Parks Road, Oxford, OX1 3RE, UK
    J Cell Sci 119:2405-13. 2006
    ..This demonstrated that orientation is independent of flagellum attachment and beating, but is influenced by constraints along its length and is entirely dependent on correct positioning at the basal plate...
  8. ncbi request reprint Cell morphogenesis of Trypanosoma brucei requires the paralogous, differentially expressed calpain-related proteins CAP5.5 and CAP5.5V
    Sofia Olego-Fernandez
    Sir William Dunn School of Pathology, University of Oxford, South Parks Road, Oxford OX1 3RE, UK
    Protist 160:576-90. 2009
    ..5 RNAi mutants. By comparison to recently described phenotypes for other procyclic trypanosome RNAi mutants, likely functions for CAP5.5 and CAP5.5V are discussed...
  9. pmc Detailed interrogation of trypanosome cell biology via differential organelle staining and automated image analysis
    Richard J Wheeler
    The Sir William Dunn School of Pathology, University of Oxford, South Parks Road, Oxford, OX1 3RE, UK
    BMC Biol 10:1. 2012
    ..This presents a technical challenge. Accurate identification and quantitation of the DNA content of these organelles is a central requirement of any automated analysis method...
  10. doi request reprint The evolution of land plant cilia
    Matthew E Hodges
    Department of Plant Sciences, South Parks Road, University of Oxford, Oxford OX1 3RB, UK
    New Phytol 195:526-40. 2012
    ....
  11. pmc Conservation of ciliary proteins in plants with no cilia
    Matthew E Hodges
    Department of Plant Sciences, University of Oxford, South Parks Rd, Oxford OX1 3RB, UK
    BMC Plant Biol 11:185. 2011
    ..During these evolutionary losses, proteins with ancestral ciliary functions may be lost or co-opted into different functions...
  12. ncbi request reprint Flagellar and ciliary beating in trypanosome motility
    Catarina Gadelha
    Sir William Dunn School of Pathology, University of Oxford, Oxford, United Kingdom
    Cell Motil Cytoskeleton 64:629-43. 2007
    ....
  13. pmc Nesprin-2 interacts with meckelin and mediates ciliogenesis via remodelling of the actin cytoskeleton
    Helen R Dawe
    Sir William Dunn School of Pathology, University of Oxford, South Parks Road, Oxford OX1 3RE, UK
    J Cell Sci 122:2716-26. 2009
    ....
  14. pmc A Trypanosoma brucei protein required for maintenance of the flagellum attachment zone and flagellar pocket ER domains
    Sylvain Lacomble
    Sir William Dunn School of Pathology, University of Oxford, Oxford OX1 3RE, United Kingdom
    Protist 163:602-15. 2012
    ..Nevertheless, depletion of TbVAP did not affect cell viability or cell cycle progression...
  15. pmc The trypanosomatid-specific N terminus of RPA2 is required for RNA polymerase I assembly, localization, and function
    Jan Peter Daniels
    Sir William Dunn School of Pathology, University of Oxford, Oxford, United Kingdom
    Eukaryot Cell 11:662-72. 2012
    ....
  16. pmc RNA interference mutant induction in vivo demonstrates the essential nature of trypanosome flagellar function during mammalian infection
    Samantha Griffiths
    Sir William Dunn School of Pathology, University of Oxford, South Parks Road, Oxford OX1 3RE, United Kingdom
    Eukaryot Cell 6:1248-50. 2007
    ..This validation provides a paradigm for the flagellum as a target for future assays and interventions against this human pathogen...
  17. pmc Three-dimensional cellular architecture of the flagellar pocket and associated cytoskeleton in trypanosomes revealed by electron microscope tomography
    Sylvain Lacomble
    Sir William Dunn School of Pathology, University of Oxford, Oxford OX1 3RE, UK
    J Cell Sci 122:1081-90. 2009
    ..Overall, our studies reveal a highly organised, yet dynamic, area of cytoplasm and will be informative in understanding its function...
  18. doi request reprint Bioinformatic insights to the ESAG5 and GRESAG5 gene families in kinetoplastid parasites
    Amy R Barker
    Sir William Dunn School of Pathology, University of Oxford, South Parks Road, Oxford OX1 3RE, UK
    Mol Biochem Parasitol 162:112-22. 2008
    ..Together, these results provide insights into the structure and evolution of an important extended gene family, and present a number of testable hypotheses which will aid in elucidating the function of ESAG5...
  19. pmc Reconstructing the evolutionary history of the centriole from protein components
    Matthew E Hodges
    Sir William Dunn School of Pathology, University of Oxford, South Parks Road, Oxford OX1 3RE, UK
    J Cell Sci 123:1407-13. 2010
    ..Finally, we demonstrate a correlation between the presence of specific centriolar proteins and eye evolution. This correlation is used to predict proteins with functions in the development of ciliary, but not rhabdomeric, eyes...
  20. pmc Evolutionary cell biology of chromosome segregation: insights from trypanosomes
    Bungo Akiyoshi
    Sir William Dunn School of Pathology, University of Oxford, Oxford OX1 3RE, UK
    Open Biol 3:130023. 2013
    ..brucei should reveal fundamental requirements for the eukaryotic segregation machinery, and may also provide hints about the origin and evolution of the segregation apparatus...
  21. pmc Cryptic paraflagellar rod in endosymbiont-containing kinetoplastid protozoa
    Catarina Gadelha
    Sir William Dunn School of Pathology, University of Oxford, South Parks Road, Oxford OX1 3RE, United Kingdom
    Eukaryot Cell 4:516-25. 2005
    ..Moreover, although PFR proteins have been conserved in evolution, primary sequence differences contribute to particular PFR morphotypes characteristic of different kinetoplastid species...
  22. pmc The kinetoplast duplication cycle in Trypanosoma brucei is orchestrated by cytoskeleton-mediated cell morphogenesis
    Eva Gluenz
    Sir William Dunn School of Pathology, University of Oxford, South Parks Road, Oxford OX1 3RE, United Kingdom
    Mol Cell Biol 31:1012-21. 2011
    ..This new view of the complexities of kinetoplast duplication emphasizes the dependencies between the dynamic remodelling of the cytoskeleton and the inheritance of the mitochondrial genome...
  23. pmc The hydrocephalus inducing gene product, Hydin, positions axonemal central pair microtubules
    Helen R Dawe
    Sir William Dunn School of Pathology, University of Oxford, South Parks Road, Oxford, OX1 3RE, UK
    BMC Biol 5:33. 2007
    ..Mutations in hydin in hy3 mice cause lethal communicating hydrocephalus with early onset. Hydin was recently identified as an axonemal protein; however, its function is as yet unknown...
  24. pmc Beyond 9+0: noncanonical axoneme structures characterize sensory cilia from protists to humans
    Eva Gluenz
    Sir William Dunn School of Pathology, University of Oxford, South Parks Road, Oxford OX1 3RE, UK
    FASEB J 24:3117-21. 2010
    ..We propose that the main function of the amastigote flagellum is to act as a sensory organelle with important functions in host-parasite interactions and signaling in the intracellular stage of the L. mexicana life cycle...
  25. pmc Discovery of unconventional kinetochores in kinetoplastids
    Bungo Akiyoshi
    Sir William Dunn School of Pathology, University of Oxford, Oxford OX1 3RE, UK Department of Biochemistry, University of Oxford, Oxford OX1 3QU, UK Electronic address
    Cell 156:1247-58. 2014
    ..We propose that kinetoplastids build kinetochores using a distinct set of proteins. These findings provide important insights into the longstanding problem of the position of the root of the eukaryotic tree of life...
  26. pmc Functional characterization of cohesin subunit SCC1 in Trypanosoma brucei and dissection of mutant phenotypes in two life cycle stages
    Eva Gluenz
    Sir William Dunn School of Pathology, University of Oxford, South Parks Road, Oxford OX1 3RE, UK
    Mol Microbiol 69:666-80. 2008
    ..In contrast, cytokinesis was incomplete in bloodstream forms, where cleavage was initiated but cells failed to progress to abscission. Kinetoplast duplication was uninterrupted resulting in cells with multiple kinetoplasts and flagella...
  27. pmc Membrane domains and flagellar pocket boundaries are influenced by the cytoskeleton in African trypanosomes
    Catarina Gadelha
    Sir William Dunn School of Pathology, University of Oxford, Oxford OX1 3RE, United Kingdom
    Proc Natl Acad Sci U S A 106:17425-30. 2009
    ..These microtubules traverse a distinct boundary and associate with a channel that connects the FP lumen to the extracellular space, suggesting that the channel is the major transport route into the FP...
  28. pmc The cell cycle of Leishmania: morphogenetic events and their implications for parasite biology
    Richard J Wheeler
    The Sir William Dunn School of Pathology, University of Oxford, South Parks Road, Oxford OX1 3RE, UK
    Mol Microbiol 79:647-62. 2011
    ..This data set therefore provides a platform which will be of use for post-genomic analyses of Leishmania cell biology in relation to differentiation and infection...
  29. pmc Comparative genomics and concerted evolution of beta-tubulin paralogs in Leishmania spp
    Andrew P Jackson
    Sir William Dunn School of Pathology, University of Oxford, South Parks Road, Oxford, OX1 3RE, UK
    BMC Genomics 7:137. 2006
    ..infantum and L. braziliensis. Untranscribed regions and coding sequences for each isoform were compared within and between species in relation to the known diversity of beta-tubulin transcripts in Leishmania spp...
  30. ncbi request reprint Trypanosome IFT mutants provide insight into the motor location for mobility of the flagella connector and flagellar membrane formation
    Jacqueline A Davidge
    Sir William Dunn School of Pathology, University of Oxford, South Parks Road, Oxford, OX1 3RE, UK
    J Cell Sci 119:3935-43. 2006
    ..Movement of the FC in the apparent absence of intraflagellar transport raises the possibility of a new form of motility within a eukaryotic flagellum...
  31. ncbi request reprint Intracellular positioning of isoforms explains an unusually large adenylate kinase gene family in the parasite Trypanosoma brucei
    Michael L Ginger
    Sir William Dunn School of Pathology, University of Oxford, South Parks Road, Oxford, OX1 3RE, United Kingdom
    J Biol Chem 280:11781-9. 2005
    ..The anchoring of specific adenylate kinases within two distinct flagellar structures provides a paradigm for metabolic organization and efficiency in other flagellates...
  32. ncbi request reprint WCB is a C2 domain protein defining the plasma membrane - sub-pellicular microtubule corset of kinetoplastid parasites
    Andrea Baines
    Sir William Dunn School of Pathology, University of Oxford, South Parks Road, Oxford OX1 3RE, UK
    Protist 159:115-25. 2008
    ..Failures in cytokinesis and zoid production are also evident. Furthermore, electron microscopy reveals that RNAi-induced trypanosomes lose local plasma membrane to microtubule corset integrity...
  33. doi request reprint An expanded family of proteins with BPI/LBP/PLUNC-like domains in trypanosome parasites: an association with pathogenicity?
    Eva Gluenz
    Sir William Dunn School of Pathology, University of Oxford, South Parks Road, Oxford OX1 3RE, UK
    Biochem Soc Trans 39:966-70. 2011
    ..Current work focuses on the elucidation of possible roles for this gene family in infection. This is likely to provide novel insights into the evolution of the BPI/LBP/PLUNC-like domains...
  34. pmc The evolution of the cytoskeleton
    Bill Wickstead
    Sir William Dunn School of Pathology, University of Oxford, Oxford OX1 3RE, England, UK
    J Cell Biol 194:513-25. 2011
    ..Much of this complexity evolved before the last common ancestor of eukaryotes. The distribution of cytoskeletal filaments puts constraints on the likely prokaryotic line that made this leap of eukaryogenesis...
  35. ncbi request reprint Centriole/basal body morphogenesis and migration during ciliogenesis in animal cells
    Helen R Dawe
    Sir William Dunn School of Pathology, University of Oxford, South Parks Road, Oxford, OX1 3RE, UK
    J Cell Sci 120:7-15. 2007
    ....
  36. ncbi request reprint The Meckel-Gruber Syndrome proteins MKS1 and meckelin interact and are required for primary cilium formation
    Helen R Dawe
    Sir William Dunn School of Pathology, University of Oxford, South Parks Road, Oxford OX1 3RE, UK
    Hum Mol Genet 16:173-86. 2007
    ..These results suggest that MKS proteins mediate a fundamental developmental stage of ciliary formation and epithelial morphogenesis...
  37. pmc Dyneins across eukaryotes: a comparative genomic analysis
    Bill Wickstead
    Sir William Dunn School of Pathology, University of Oxford, South Parks Road, Oxford, OX1 3RE, UK
    Traffic 8:1708-21. 2007
    ..One diatom species builds motile axonemes without any inner-arm dyneins (IAD), and the unexpected conservation of IAD I1 in non-flagellate algae and LC8 (DYNLL1/2) in all lineages reveals a surprising fluidity to dynein function...
  38. ncbi request reprint A repetitive protein essential for the flagellum attachment zone filament structure and function in Trypanosoma brucei
    Sue Vaughan
    Sir William Dunn School of Pathology, University of Oxford, South Parks Road, Oxford OX1 3RE, UK
    Protist 159:127-36. 2008
    ..The cytokinesis defects provide further evidence for the role of an attached flagellum in cellular morphogenesis in these trypanosomes...
  39. pmc The limits on trypanosomatid morphological diversity
    Richard John Wheeler
    Sir William Dunn School of Pathology, University of Oxford, Oxford, United Kingdom
    PLoS ONE 8:e79581. 2013
    ....
  40. doi request reprint Cytokinesis in Trypanosoma brucei differs between bloodstream and tsetse trypomastigote forms: implications for microtubule-based morphogenesis and mutant analysis
    Richard J Wheeler
    Sir William Dunn School of Pathology, University of Oxford, South Parks Road, Oxford, OX1 3RE, UK
    Mol Microbiol 90:1339-55. 2013
    ..These discoveries are important tools to explain differences between bloodstream and procyclic form RNAi phenotypes involving organelle mis-positioning during cell division and cytokinesis defects. ..
  41. doi request reprint Cytokinesis in trypanosomes
    Helen Farr
    Sir William Dunn School of Pathology, University of Oxford, South Parks Road, Oxford OX1 3RE, United Kingdom
    Cytoskeleton (Hoboken) 69:931-41. 2012
    ..Recent advances in three-dimensional imaging techniques have improved our understanding of the mechanisms driving cytokinesis and are likely to yield further insights in the future...
  42. pmc Patterns of kinesin evolution reveal a complex ancestral eukaryote with a multifunctional cytoskeleton
    Bill Wickstead
    University of Oxford, UK
    BMC Evol Biol 10:110. 2010
    ..Here, we have used the kinesin motor repertoire of 45 extant eukaryotes to infer the ancestral state of this superfamily in the last common eukaryotic ancestor (LCEA)...
  43. ncbi request reprint The Parkin co-regulated gene product, PACRG, is an evolutionarily conserved axonemal protein that functions in outer-doublet microtubule morphogenesis
    Helen R Dawe
    Sir William Dunn School of Pathology, University of Oxford, South Parks Road, Oxford, OX1 3RE, UK
    J Cell Sci 118:5421-30. 2005
    ..Our results provide the first evidence for PACRG function within the axoneme, where we suggest that PACRG acts to maintain functional stability of the axonemal outer doublets of both motile and sensory cilia and flagella...
  44. doi request reprint Ultrastructural investigation methods for Trypanosoma brucei
    Johanna L Höög
    Sir William Dunn School of Pathology, University of Oxford, Oxford OX13RE, United Kingdom
    Methods Cell Biol 96:175-96. 2010
    ..We also introduce a novel high-pressure freezing protocol, which followed by rapid freeze substitution and HM20 embedding generates T. brucei samples displaying good cell morphology, which are suitable for immunocytochemistry...
  45. pmc Variant surface glycoprotein RNA interference triggers a precytokinesis cell cycle arrest in African trypanosomes
    Karen Sheader
    Peter Medawar Building for Pathogen Research, University of Oxford, South Parks Road, Oxford OX1 3SY, United Kingdom
    Proc Natl Acad Sci U S A 102:8716-21. 2005
    ....
  46. pmc The paraflagellar rod of kinetoplastid parasites: from structure to components and function
    Neil Portman
    Sir William Dunn School of Pathology, University of Oxford, Oxford OX13RE, UK
    Int J Parasitol 40:135-48. 2010
    ....
  47. pmc Cell biology of the trypanosome genome
    Jan Peter Daniels
    University of Oxford, Oxford, United Kingdom
    Microbiol Mol Biol Rev 74:552-69. 2010
    ..We also compare this nuclear organization to those in other systems in order to shed light on the evolution of nuclear architecture in eukaryotes...
  48. pmc Functional genomics in Trypanosoma brucei: a collection of vectors for the expression of tagged proteins from endogenous and ectopic gene loci
    Steven Kelly
    Sir William Dunn School of Pathology, University of Oxford, South Parks Road, Oxford OX1 3RE, UK
    Mol Biochem Parasitol 154:103-9. 2007
  49. pmc Identification of a crenarchaeal orthologue of Elf1: implications for chromatin and transcription in Archaea
    Jan Peter Daniels
    Sir William Dunn School of Pathology, University of Oxford, South Parks Road, Oxford, OX1 3RE, UK
    Biol Direct 4:24. 2009
    ..Our discovery has implications for the relationship of chromatin and transcription in Archaea and the evolution of these processes in eukaryotes...
  50. pmc Protein targeting of an unusual, evolutionarily conserved adenylate kinase to a eukaryotic flagellum
    Timothy J Pullen
    Sir William Dunn School of Pathology, University of Oxford, Oxford OX1 3RE, United Kingdom
    Mol Biol Cell 15:3257-65. 2004
    ....
  51. pmc A "holistic" kinesin phylogeny reveals new kinesin families and predicts protein functions
    Bill Wickstead
    Sir William Dunn School of Pathology, University of Oxford, Oxford OX1 3RE, United Kingdom
    Mol Biol Cell 17:1734-43. 2006
    ..Finally, we present a set of hidden Markov models that can reliably place most new kinesin sequences into families, even when from an organism at a great evolutionary distance from those in the analysis...
  52. ncbi request reprint Relationships between the major kinetoplastid paraflagellar rod proteins: a consolidating nomenclature
    Catarina Gadelha
    Sir William Dunn School of Pathology, University of Oxford, UK
    Mol Biochem Parasitol 136:113-5. 2004
  53. doi request reprint The structural mechanics of cell division in Trypanosoma brucei
    Sue Vaughan
    Sir William Dunn School of Pathology, University of Oxford, South Parks Road, Oxford OX1 3RE, UK
    Biochem Soc Trans 36:421-4. 2008
    ..Here we outline our current understanding of some of the structural aspects of cell division in this fascinating organism...
  54. pmc Structural asymmetry and discrete nucleic acid subdomains in the Trypanosoma brucei kinetoplast
    Eva Gluenz
    Sir William Dunn School of Pathology, University of Oxford, South Parks Road, Oxford OX1 3RE, UK
    Mol Microbiol 64:1529-39. 2007
    ..We also describe a hitherto unrecognized, intramitochondrial, filamentous structure rich in basic proteins that links the kDNA discs during their segregation and is maintained between them for an extended period of the cell cycle...
  55. ncbi request reprint Molecular evolution of FtsZ protein sequences encoded within the genomes of archaea, bacteria, and eukaryota
    Sue Vaughan
    Sir William Dunn School of Pathology, University of Oxford, South Parks Road, Oxford OX1 3RE, UK
    J Mol Evol 58:19-29. 2004
    ..In addition, we identify "FtsZ-like" sequences from Bacteria and Archaea that, while showing significant sequence similarity to FtsZs, are unlikely to bind and hydrolyze GTP...
  56. doi request reprint Proteomics and the Trypanosoma brucei cytoskeleton: advances and opportunities
    Neil Portman
    The Sir William Dunn School of Pathology and Oxford Centre for Integrative Systems Biology, University of Oxford, Oxford, OX1 3RE, UK
    Parasitology 139:1168-77. 2012
    ....
  57. pmc The small chromosomes of Trypanosoma brucei involved in antigenic variation are constructed around repetitive palindromes
    Bill Wickstead
    Sir William Dunn School of Pathology, University of Oxford, Oxford, OX1 3RE, United Kingdom
    Genome Res 14:1014-24. 2004
    ..Moreover, palindromy appears to be a feature of (peri)centromeres in other species that can be easily overlooked. We propose that sequence inversion is one of the higher-order sequence motifs that confer chromosomal stability...
  58. ncbi request reprint The flagella connector of Trypanosoma brucei: an unusual mobile transmembrane junction
    Laura J Briggs
    Sir William Dunn School of Pathology, University of Oxford, South Parks Road, Oxford, OX1 3RE, UK
    J Cell Sci 117:1641-51. 2004
    ..The flagella connector represents a novel form of cellular junction that is both dynamic and mobile...
  59. pmc Repetitive elements in genomes of parasitic protozoa
    Bill Wickstead
    Sir William Dunn School of Pathology, University of Oxford, Oxford OX1 3RE, United Kingdom
    Microbiol Mol Biol Rev 67:360-75, table of contents. 2003
    ..The story which emerges is one of opportunism and upheaval which have been employed to add genetic diversity and genomic flexibility...
  60. ncbi request reprint The trypanosome flagellum
    Sue Vaughan
    Sir William Dunn School of Pathology, University of Oxford, South Parks Road, Oxford OX1 3RE, UK
    J Cell Sci 116:757-9. 2003
  61. ncbi request reprint The mitotic stability of the minichromosomes of Trypanosoma brucei
    Bill Wickstead
    Sir William Dunn School of Pathology, University of Oxford, South Parks Road, OX1 3RE, Oxford, UK
    Mol Biochem Parasitol 132:97-100. 2003
  62. pmc Chromosome-wide analysis of gene function by RNA interference in the african trypanosome
    Chandra Subramaniam
    School of Biological Sciences, University of Manchester, Oxford Road, Manchester, United Kingdom
    Eukaryot Cell 5:1539-49. 2006
    ....
  63. ncbi request reprint Gamma-tubulin functions in the nucleation of a discrete subset of microtubules in the eukaryotic flagellum
    Paul G McKean
    Department of Biological Sciences, Lancaster University, Lancaster, LA1 4YQ, Lancashire, United Kingdom
    Curr Biol 13:598-602. 2003
    ....
  64. ncbi request reprint More than one way to build a flagellum: comparative genomics of parasitic protozoa
    Laura J Briggs
    Curr Biol 14:R611-2. 2004
  65. pmc An evolutionarily conserved coiled-coil protein implicated in polycystic kidney disease is involved in basal body duplication and flagellar biogenesis in Trypanosoma brucei
    Gareth W Morgan
    Department of Pathology, University of Cambridge, Tennis Court Road, Cambridge, United Kingdom
    Mol Cell Biol 25:3774-83. 2005
    ..These data further underscore the role of aberrant flagellar biogenesis as a disease mechanism...
  66. ncbi request reprint The genome of the African trypanosome Trypanosoma brucei
    Matthew Berriman
    Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton CB10 1SA, UK
    Science 309:416-22. 2005
    ..brucei and the greatest in L. major. Horizontal transfer of genes of bacterial origin has contributed to some of the metabolic differences in these parasites, and a number of novel potential drug targets have been identified...
  67. ncbi request reprint Evolutionary relationships and protein domain architecture in an expanded calpain superfamily in kinetoplastid parasites
    Klaus Ersfeld
    Department of Biological Sciences, University of Hull, Hull, HU6 7RX, UK
    J Mol Evol 61:742-57. 2005
    ..The discovery of this surprisingly large family of calpain-like proteins in lower eukaryotes that combines novel and conserved sequence modules contributes to our understanding of the evolution of this abundant protein family...
  68. ncbi request reprint Flagellar motility is required for the viability of the bloodstream trypanosome
    Richard Broadhead
    Department of Biological Sciences, Lancaster University, Lancaster LA1 4YQ, UK
    Nature 440:224-7. 2006
    ..A postgenomic meta-analysis, comparing the evolutionarily ancient trypanosome with other eukaryotes including humans, identifies numerous trypanosome-specific flagellar proteins, suggesting new avenues for selective intervention...
  69. ncbi request reprint A post-assembly structural modification to the lumen of flagellar microtubule doublets
    Sue Vaughan
    Curr Biol 16:R449-50. 2006
  70. ncbi request reprint Evidence for a sliding-resistance at the tip of the trypanosome flagellum
    David Woolley
    Department of Physiology, School of Medical Sciences, University of Bristol, Bristol, United Kingdom
    Cell Motil Cytoskeleton 63:741-6. 2006
    ..Thus, it can be assumed that in trypanosomes microtubule sliding at the flagellar tip is resisted sufficiently to permit bend formation...
  71. pmc Identification and characterization of two trypanosome TFIIS proteins exhibiting particular domain architectures and differential nuclear localizations
    Pierrick Uzureau
    Laboratoire de Parasitologie Moleculaire, ULB IBMM, rue des Pr Jeneer et Brachet 12, B 6041 Gosselies, Belgium
    Mol Microbiol 69:1121-36. 2008
    ..Repetitive failure to generate a double knockout of TbTFIIS1 and TbTFIIS2-1 strongly suggests synthetical lethality and thus an essential function shared by the two proteins in trypanosome growth...
  72. ncbi request reprint Integration of pZJM library plasmids into unexpected locations in the Trypanosoma brucei genome
    Shawn A Motyka
    Department of Biological Chemistry, Johns Hopkins School of Medicine, 725 N Wolfe Street, Baltimore, MD 21205, USA
    Mol Biochem Parasitol 134:163-7. 2004
  73. ncbi request reprint An essential quality control mechanism at the eukaryotic basal body prior to intraflagellar transport
    Angela Stephan
    Biomedical Sciences Unit, Department of Biological Sciences, Lancaster University, Lancaster, LA1 4YQ, UK
    Traffic 8:1323-30. 2007
    ....
  74. ncbi request reprint The genome sequence of Trypanosoma cruzi, etiologic agent of Chagas disease
    Najib M El-Sayed
    Department of Parasite Genomics, Institute for Genomic Research, Rockville, MD 20850, USA
    Science 309:409-15. 2005
    ....
  75. pmc Diversification of function by different isoforms of conventionally shared RNA polymerase subunits
    Sara Devaux
    Institute for Molecular Biology and Medicine, Universite Libre de Bruxelles, 6041 Gosselies, Belgium
    Mol Biol Cell 18:1293-301. 2007
    ....
  76. pmc The DNA sequence of chromosome I of an African trypanosome: gene content, chromosome organisation, recombination and polymorphism
    Neil Hall
    The Wellcome Trust Sanger Institute, The Wellcome Trust Genome Campus, Hinxton CB10 1SA, UK
    Nucleic Acids Res 31:4864-73. 2003
    ..Genetic polymorphisms are exceptionally rare in sequences located within and around the strand-switches between several gene clusters...
  77. pmc The frequency of gene targeting in Trypanosoma brucei is independent of target site copy number
    Bill Wickstead
    School of Biological Sciences, University of Manchester, 2 205 Stopford Building, Oxford Road, Manchester M13 9PT, UK
    Nucleic Acids Res 31:3993-4000. 2003
    ..It is also not seen in the related parasite Leishmania, a distinction that may be the consequence of the different usage of recombination within the mechanisms of pathogenicity in the two species...
  78. pmc A high-order trans-membrane structural linkage is responsible for mitochondrial genome positioning and segregation by flagellar basal bodies in trypanosomes
    Emmanuel O Ogbadoyi
    School of Biological Sciences, University of Manchester, England
    Mol Biol Cell 14:1769-79. 2003
    ..We suggest that this complex may represent an extreme form of a more generally occurring mitochondrion/cytoskeleton interaction...
  79. ncbi request reprint Targeting of a tetracycline-inducible expression system to the transcriptionally silent minichromosomes of Trypanosoma brucei
    Bill Wickstead
    School of Biological Sciences, University of Manchester, 2 205 Stopford Building, Oxford Road, Manchester M13 9PT, UK
    Mol Biochem Parasitol 125:211-6. 2002
  80. ncbi request reprint Asymmetric cell division as a route to reduction in cell length and change in cell morphology in trypanosomes
    Reuben Sharma
    Department of Biochemistry, University of Cambridge, 80 Tennis Court Road, Cambridge CB2 1GA, UK
    Protist 159:137-51. 2008
    ..Thus, the asymmetric cell division cycle provides a mechanism for a change in cell morphology and also an explanation for how a reduction in cell length can occur in a cell shaped by a stable microtubule array...