Research Topics
Genomes and Genes
| Nicholas D E GreeneSummaryAffiliation: University College London Country: UK Publications
| Collaborators
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Detail Information
Publications
Mouse models of neural tube defects: investigating preventive mechanismsNicholas D E Greene
Neural Development Unit, Institute of Child Health, University College London, UK
Am J Med Genet C Semin Med Genet 135:31-41. 2005..Overall, the data from mouse models suggests that a broad-based in utero therapy may offer scope for prevention of a greater proportion of NTD than is currently possible...
Development of the vertebrate central nervous system: formation of the neural tubeNicholas D E Greene
Neural Development Unit, UCL Institute of Child Health, London, UK
Prenat Diagn 29:303-11. 2009..We describe the process of neural tube formation, discuss the cellular mechanisms involved and highlight recent findings that provide links between molecular signaling pathways and morphogenetic tissue movements...- Differential protein expression at the stage of neural tube closure in the mouse embryoNicholas D E Greene
Neural Development Unit, Institute of Child Health, University College London, United Kingdom
J Biol Chem 277:41645-51. 2002..Additional proteins of unknown function were identified, such as Copine 1 and PICOT, whose developmental regulation was previously unsuspected... - Understanding the causes and prevention of neural tube defects: Insights from the splotch mouse modelNicholas D E Greene
Neural Development Unit, Institute of Child Health, University College London, London, United Kingdom
Birth Defects Res A Clin Mol Teratol 85:322-30. 2009.... - Homocysteine is embryotoxic but does not cause neural tube defects in mouse embryosNicholas D E Greene
Neural Development Unit, Institute of Child Health, 30 Guilford Street, London, WC1N 1EH, UK
Anat Embryol (Berl) 206:185-91. 2003..These observations suggest that homocysteine is unlikely to be a direct cause of NTD in humans. Rather, the elevated levels of homocysteine in human NTD pregnancies may reflect a disturbance in folate-related metabolism... - Proteome changes associated with hippocampal MRI abnormalities in the lithium pilocarpine-induced model of convulsive status epilepticusNicholas D E Greene
Neural Development Unit, UCL Institute of Child Health, University College London, London, UK
Proteomics 7:1336-44. 2007..In summary, the hippocampus of rats subject to pilocarpine-induced SE exhibits specific changes in protein abundance, which likely relate to pathogenic, neuroprotective and neurogenic responses... - Neural tube defects induced by folate deficiency in mutant curly tail (Grhl3) embryos are associated with alteration in folate one-carbon metabolism but are unlikely to result from diminished methylationSandra C P de Castro
Neural Development Unit, UCL Institute of Child Health, London, UK
Birth Defects Res A Clin Mol Teratol 88:612-8. 2010.... - Abnormal folate metabolism in foetuses affected by neural tube defectsLouisa P E Dunlevy
Neural Development Unit, Institute of Child Health, University College London, UK
Brain 130:1043-9. 2007..These data suggest that as yet unrecognized genetic variants result in embryonic abnormalities of folate cycling that may be causally related to NTDs... 
The genetic background of the curly tail strain confers susceptibility to folate-deficiency-induced exencephalyKatie A Burren
Neural Development Unit, UCL Institute of Child Health, London, UK
Birth Defects Res A Clin Mol Teratol 88:76-83. 2010..The purpose of the present study was to determine whether folate deficiency can induce NTDs in mice with a permissive genetic background which do not normally exhibit defects...- Neural plate morphogenesis during mouse neurulation is regulated by antagonism of Bmp signallingPatricia Ybot-Gonzalez
Neural Development Unit, Institute of Child Health, University College London, 30 Guilford Street, London WC1N 1EH, UK
Development 134:3203-11. 2007..Our findings reveal a molecular mechanism based on antagonism of Bmp signalling that underlies the regulation of DLHP formation during mouse spinal neural tube closure... - Gene-environment interactions in the causation of neural tube defects: folate deficiency increases susceptibility conferred by loss of Pax3 functionKatie A Burren
Neural Development Unit, UCL Institute of Child Health, University College London, London, UK
Hum Mol Genet 17:3675-85. 2008..Hence, folate deficiency increases the risk of NTDs in genetically predisposed splotch embryos, probably via embryonic growth retardation... - Integrity of the methylation cycle is essential for mammalian neural tube closureLouisa P E Dunlevy
Neural Development Unit, Institute of Child Health, University College London, United Kingdom
Birth Defects Res A Clin Mol Teratol 76:544-52. 2006..We hypothesize that inhibition of the methylation cycle causes NTDs due to disruption of crucial reactions involving methylation of DNA, proteins or other biomolecules... - Convergent extension, planar-cell-polarity signalling and initiation of mouse neural tube closurePatricia Ybot-Gonzalez
Neural Development Unit, Institute of Child Health, University College London, 30 Guilford Street, London WC1N 1EH, UK
Development 134:789-99. 2007..These findings identify a cell-autonomous defect of convergent extension, requiring PCP signalling via RhoA-Rho kinase, during the development of severe neural tube defects in the mouse... - Convergent extension analysis in mouse whole embryo cultureSophie E Pryor
Neural Development Unit, Institute of Child Health, University College London, London, UK
Methods Mol Biol 839:133-46. 2012..We describe analytical methods that can reveal the abnormalities that characterise neurulation-stage embryos with defective planar cell polarity signalling, in particular the loop-tail (Lp; Vangl2) mutant... - In vivo genetic ablation by Cre-mediated expression of diphtheria toxin fragment AAnna Ivanova
Neural Development Unit, Institute of Child Health, University College London, United Kingdom
Genesis 43:129-35. 2005..We observed loss of the precise tissues in which Nkx2.5 and Wnt1 are expressed. Apart from being a general GFP reporter, the ROSA26-GFP-DTA mouse line should provide a useful resource for genetic ablation of specific groups of cells... - Increased expression of Grainyhead-like-3 rescues spina bifida in a folate-resistant mouse modelPeter Gustavsson
Neural Development Unit, Institute of Child Health, University College London, Guilford Street, London WC1N 1EH, UK
Hum Mol Genet 16:2640-6. 2007..This study provides evidence for a critical role of diminished Grhl3 expression in causing spinal NTDs in the curly tail mouse model... - Mutations in the planar cell polarity genes CELSR1 and SCRIB are associated with the severe neural tube defect craniorachischisisAlexis Robinson
UCL Institute of Child Health, London WC1N 1EH, United Kingdom
Hum Mutat 33:440-7. 2012..We conclude that missense variants in CELSR1 and SCRIB may represent a cause of CRN in humans, as in mice, with defective PCP protein trafficking to the plasma membrane a likely pathogenic mechanism... - Protein deiminases: new players in the developmentally regulated loss of neural regenerative abilitySigrun Lange
Developmental Biology Unit, UCL Institute of Child Health, London WC1N 1EH, UK
Dev Biol 355:205-14. 2011..Altogether, our findings identify PADs and deimination as developmentally regulated modulators of secondary injury response, and suggest that PADs might be valuable therapeutic targets for spinal cord injury... - Post-translational regulation of Crmp in developing and regenerating chick spinal cordStefanie Gögel
Developmental Biology Unit, UCL Institute of Child Health, London, United Kingdom
Dev Neurobiol 70:456-71. 2010.... - Apoptosis is not required for mammalian neural tube closureValentina Massa
Neural Development Unit, University College London Institute of Child Health, London, United Kingdom
Proc Natl Acad Sci U S A 106:8233-8. 2009..We conclude that apoptosis is not required for neural tube closure in the mouse embryo... - The genetic basis of mammalian neurulationAndrew J Copp
Neural Development Unit, Institute of Child Health, University College London, London WC1N 1EH, UK
Nat Rev Genet 4:784-93. 2003..Mutant mice also offer an opportunity to unravel the mechanisms by which folic acid prevents neural tube defects, and to develop new therapies for folate-resistant defects... - Specific isoforms of protein kinase C are essential for prevention of folate-resistant neural tube defects by inositolPatricia Cogram
Neural Development Unit, Institute of Child Health, 30 Guilford Street, London WC1N 1EH, UK
Hum Mol Genet 13:7-14. 2004..Hindgut cell proliferation was stimulated specifically by inositol, an effect that required activation of PKCbetaI. Our findings reveal an essential role of specific PKC isoforms in mediating the prevention of mouse NTDs by inositol... - Regional differences in the expression of laminin isoforms during mouse neural tube developmentAndrew J Copp
Neural Development Unit, Institute of Child Health, University College London, UK
Matrix Biol 30:301-9. 2011..This information paves the way towards a mechanistic analysis of basement membrane laminin function during early neural tube development in mammals... - Quantitative analysis of myo-inositol in urine, blood and nutritional supplements by high-performance liquid chromatography tandem mass spectrometryKit Yi Leung
Neural Development Unit, Institute of Child Health, University College London, 30 Guilford Street, London, WC1N 1EH, United Kingdom
J Chromatogr B Analyt Technol Biomed Life Sci 879:2759-63. 2011..1% and 3.5% respectively, while intra-assay CV was 2.3% and 3.6%. Urine and blood samples from normal individuals were analysed... - Excess methionine suppresses the methylation cycle and inhibits neural tube closure in mouse embryosLouisa P E Dunlevy
Neural Development Unit, Institute of Child Health, University College London, 30 Guilford Street, London WC1N 1EA, UK
FEBS Lett 580:2803-7. 2006..Thus, reduced cranial mesenchyme density and impairment of critical methylation reactions may contribute to development of methionine-induced NTD... - Lamin b1 polymorphism influences morphology of the nuclear envelope, cell cycle progression, and risk of neural tube defects in miceSandra C P de Castro
Neural Development Unit, UCL Institute of Child Health, University College London, London, United Kingdom
PLoS Genet 8:e1003059. 2012.... - Genetics and development of neural tube defectsAndrew J Copp
Neural Development Unit, UCL Institute of Child Health, London WC1N 1EH, UK
J Pathol 220:217-30. 2010..Folic acid-resistant cases can be prevented by inositol supplementation in mice, raising the possibility that this could lead to an additional preventive strategy for human NTDs in future... - Genetics of human neural tube defectsNicholas D E Greene
Neural Development Unit, UCL Institute of Child Health, London, UK
Hum Mol Genet 18:R113-29. 2009.... - Defining a PARticular pathway of neural tube closureAndrew J Copp
UCL Institute of Child Health, 30 Guilford Street, London WC1N 1EH, UK
Dev Cell 18:1-2. 2010.... - Dishevelled: linking convergent extension with neural tube closureAndrew J Copp
Neural Development Unit, Institute of Child Health, University College, London WC1N 1EH, UK
Trends Neurosci 26:453-5. 2003..Although bilateral neural folds form, they are abnormally far apart and cannot achieve the apposition necessary for neural tube closure... - Quantitative analysis of s-adenosylmethionine and s-adenosylhomocysteine in neurulation-stage mouse embryos by liquid chromatography tandem mass spectrometryKatie A Burren
Neural Development Unit, Institute of Child Health, University College London, 30 Guilford Street, London WC1N 1EH, United Kingdom
J Chromatogr B Analyt Technol Biomed Life Sci 844:112-8. 2006..Calibration curves suitable for the analysis of neurulation-stage mouse embryos (SAM 0.02-25.0microM, SAH 0.01-10.0microM) were linear (r(2)>0.997) with limits of detection for SAM and SAH of 10 and 2.5nmol/L, respectively... - Zic4, a zinc-finger transcription factor, is expressed in the developing mouse nervous systemCarles Gaston-Massuet
Neural Development Unit, Institute of Child Health, University College London, United Kingdom
Dev Dyn 233:1110-5. 2005..We conclude that Zic4 has an expression pattern distinct from, but partly overlapping with, other members of the Zic gene family... - Expression pattern of glypican-4 suggests multiple roles during mouse developmentPatricia Ybot-Gonzalez
Neural Development Unit, Institute of Child Health, University College London, United Kingdom
Dev Dyn 233:1013-7. 2005..Glypican-4 expression is not detected in the midline of the embryo at the stage of initiation of neural tube closure, suggesting that glypican-4 is unlikely to play an essential role in convergent extension in the mouse... - D-chiro-inositol is more effective than myo-inositol in preventing folate-resistant mouse neural tube defectsPatricia Cogram
Neural Development Unit, Institute of Child Health, University College London, UK
Hum Reprod 17:2451-8. 2002..This raises the possibility of using inositol as an adjunct therapy to folic acid for prevention of NTDs in humans... - EphrinA-EphA receptor interactions in mouse spinal neurulation: implications for neural fold fusionNoraishah M Abdul-Aziz
Neural Development Unit, UCL Institute of Child Health, London WC1N 1EH, UK
Int J Dev Biol 53:559-68. 2009.... - Magnetic resonance virtual histology for embryos: 3D atlases for automated high-throughput phenotypingJon O Cleary
Centre for Advanced Biomedical Imaging, Department of Medicine, University College London, London, UK
Neuroimage 54:769-78. 2011..Our work demonstrates the feasibility of combining high-throughput embryo MRI with automated analysis techniques to distinguish novel mouse phenotypes... - The emerging role of epigenetic mechanisms in the etiology of neural tube defectsNicholas D E Greene
Neural Development Unit, Institute of Child Health, UCL, London, UK
Epigenetics 6:875-83. 2011..Alterations in the epigenetic status can be directly modified by various environmental insults or maternal dietary factors... - Metformin retards aging in C. elegans by altering microbial folate and methionine metabolismFilipe Cabreiro
Institute of Healthy Ageing, and G E E, University College London, London WC1E 6BT, UK
Cell 153:228-39. 2013..Thus, metformin-induced alteration of microbial metabolism could contribute to therapeutic efficacy-and also to its side effects, which include folate deficiency and gastrointestinal upset... - Planar cell polarity genes and neural tube closureNaoto Ueno
Department of Developmental Biology, National Institute for Basic Biology, and SOKENDAI, Okazaki, Japan
Birth Defects Res C Embryo Today 69:318-24. 2003..Therefore, understanding the mechanisms underlying the establishment of cell polarity in Drosophila may provide important clues to the molecular basis of NTDs... - Folic acid prevents exencephaly in Cited2 deficient miceJuan Pedro Martinez Barbera
MRC Centre for Developmental Neurobiology, 4th floor New Hunt s House, King s College London, Guy s Campus, London Bridge, London SE1 1UL, UK
Hum Mol Genet 11:283-93. 2002..To our knowledge, the Cited2 mouse represents the first genetic model in which folic acid can prevent a defect in neural tube closure by a mechanism other than the neutralization of a defect in folate homeostasis... - Retinal pathology and function in a Cln3 knockout mouse model of juvenile Neuronal Ceroid Lipofuscinosis (batten disease)Gail M Seigel
Department of Ophthalmology, University of Rochester School of Medicine, New York, USA
Mol Cell Neurosci 19:515-27. 2002.... - Grainyhead genes and mammalian neural tube closurePeter Gustavsson
Department of Molecular Medicine and Surgery, Karolinska Institute, Stockholm, Sweden
Birth Defects Res A Clin Mol Teratol 82:728-35. 2008....