Nicholas D E Greene

Summary

Affiliation: University College London
Country: UK

Publications

  1. pmc Over-expression of Grhl2 causes spina bifida in the Axial defects mutant mouse
    Madeleine R Brouns
    Department of Anatomy and Embryology, Maastricht University Medical Center, University of Maastricht, PO Box 616, 6200 MD, Maastricht, The Netherlands
    Hum Mol Genet 20:1536-46. 2011
  2. ncbi request reprint Mouse models of neural tube defects: investigating preventive mechanisms
    Nicholas D E Greene
    Neural Development Unit, Institute of Child Health, University College London, UK
    Am J Med Genet C Semin Med Genet 135:31-41. 2005
  3. ncbi request reprint Development of the vertebrate central nervous system: formation of the neural tube
    Nicholas D E Greene
    Neural Development Unit, UCL Institute of Child Health, London, UK
    Prenat Diagn 29:303-11. 2009
  4. ncbi request reprint Differential protein expression at the stage of neural tube closure in the mouse embryo
    Nicholas D E Greene
    Neural Development Unit, Institute of Child Health, University College London, United Kingdom
    J Biol Chem 277:41645-51. 2002
  5. ncbi request reprint Proteome changes associated with hippocampal MRI abnormalities in the lithium pilocarpine-induced model of convulsive status epilepticus
    Nicholas D E Greene
    Neural Development Unit, UCL Institute of Child Health, University College London, London, UK
    Proteomics 7:1336-44. 2007
  6. ncbi request reprint Homocysteine is embryotoxic but does not cause neural tube defects in mouse embryos
    Nicholas D E Greene
    Neural Development Unit, Institute of Child Health, 30 Guilford Street, London, WC1N 1EH, UK
    Anat Embryol (Berl) 206:185-91. 2003
  7. ncbi request reprint Understanding the causes and prevention of neural tube defects: Insights from the splotch mouse model
    Nicholas D E Greene
    Neural Development Unit, Institute of Child Health, University College London, London, United Kingdom
    Birth Defects Res A Clin Mol Teratol 85:322-30. 2009
  8. pmc Neural tube defects induced by folate deficiency in mutant curly tail (Grhl3) embryos are associated with alteration in folate one-carbon metabolism but are unlikely to result from diminished methylation
    Sandra C P de Castro
    Neural Development Unit, UCL Institute of Child Health, London, UK
    Birth Defects Res A Clin Mol Teratol 88:612-8. 2010
  9. pmc The genetic background of the curly tail strain confers susceptibility to folate-deficiency-induced exencephaly
    Katie A Burren
    Neural Development Unit, UCL Institute of Child Health, London, UK
    Birth Defects Res A Clin Mol Teratol 88:76-83. 2010
  10. pmc Lamin b1 polymorphism influences morphology of the nuclear envelope, cell cycle progression, and risk of neural tube defects in mice
    Sandra C P de Castro
    Neural Development Unit, UCL Institute of Child Health, University College London, London, United Kingdom
    PLoS Genet 8:e1003059. 2012

Collaborators

Detail Information

Publications51

  1. pmc Over-expression of Grhl2 causes spina bifida in the Axial defects mutant mouse
    Madeleine R Brouns
    Department of Anatomy and Embryology, Maastricht University Medical Center, University of Maastricht, PO Box 616, 6200 MD, Maastricht, The Netherlands
    Hum Mol Genet 20:1536-46. 2011
    ..Overall, our data suggest that defects in Axd mutant embryos result from over-expression of Grhl2...
  2. ncbi request reprint Mouse models of neural tube defects: investigating preventive mechanisms
    Nicholas D E Greene
    Neural Development Unit, Institute of Child Health, University College London, UK
    Am J Med Genet C Semin Med Genet 135:31-41. 2005
    ..Overall, the data from mouse models suggests that a broad-based in utero therapy may offer scope for prevention of a greater proportion of NTD than is currently possible...
  3. ncbi request reprint Development of the vertebrate central nervous system: formation of the neural tube
    Nicholas D E Greene
    Neural Development Unit, UCL Institute of Child Health, London, UK
    Prenat Diagn 29:303-11. 2009
    ..We describe the process of neural tube formation, discuss the cellular mechanisms involved and highlight recent findings that provide links between molecular signaling pathways and morphogenetic tissue movements...
  4. ncbi request reprint Differential protein expression at the stage of neural tube closure in the mouse embryo
    Nicholas D E Greene
    Neural Development Unit, Institute of Child Health, University College London, United Kingdom
    J Biol Chem 277:41645-51. 2002
    ..Additional proteins of unknown function were identified, such as Copine 1 and PICOT, whose developmental regulation was previously unsuspected...
  5. ncbi request reprint Proteome changes associated with hippocampal MRI abnormalities in the lithium pilocarpine-induced model of convulsive status epilepticus
    Nicholas D E Greene
    Neural Development Unit, UCL Institute of Child Health, University College London, London, UK
    Proteomics 7:1336-44. 2007
    ..In summary, the hippocampus of rats subject to pilocarpine-induced SE exhibits specific changes in protein abundance, which likely relate to pathogenic, neuroprotective and neurogenic responses...
  6. ncbi request reprint Homocysteine is embryotoxic but does not cause neural tube defects in mouse embryos
    Nicholas D E Greene
    Neural Development Unit, Institute of Child Health, 30 Guilford Street, London, WC1N 1EH, UK
    Anat Embryol (Berl) 206:185-91. 2003
    ..These observations suggest that homocysteine is unlikely to be a direct cause of NTD in humans. Rather, the elevated levels of homocysteine in human NTD pregnancies may reflect a disturbance in folate-related metabolism...
  7. ncbi request reprint Understanding the causes and prevention of neural tube defects: Insights from the splotch mouse model
    Nicholas D E Greene
    Neural Development Unit, Institute of Child Health, University College London, London, United Kingdom
    Birth Defects Res A Clin Mol Teratol 85:322-30. 2009
    ....
  8. pmc Neural tube defects induced by folate deficiency in mutant curly tail (Grhl3) embryos are associated with alteration in folate one-carbon metabolism but are unlikely to result from diminished methylation
    Sandra C P de Castro
    Neural Development Unit, UCL Institute of Child Health, London, UK
    Birth Defects Res A Clin Mol Teratol 88:612-8. 2010
    ....
  9. pmc The genetic background of the curly tail strain confers susceptibility to folate-deficiency-induced exencephaly
    Katie A Burren
    Neural Development Unit, UCL Institute of Child Health, London, UK
    Birth Defects Res A Clin Mol Teratol 88:76-83. 2010
    ..The purpose of the present study was to determine whether folate deficiency can induce NTDs in mice with a permissive genetic background which do not normally exhibit defects...
  10. pmc Lamin b1 polymorphism influences morphology of the nuclear envelope, cell cycle progression, and risk of neural tube defects in mice
    Sandra C P de Castro
    Neural Development Unit, UCL Institute of Child Health, University College London, London, United Kingdom
    PLoS Genet 8:e1003059. 2012
    ....
  11. ncbi request reprint Abnormal folate metabolism in foetuses affected by neural tube defects
    Louisa P E Dunlevy
    Neural Development Unit, Institute of Child Health, University College London, UK
    Brain 130:1043-9. 2007
    ..These data suggest that as yet unrecognized genetic variants result in embryonic abnormalities of folate cycling that may be causally related to NTDs...
  12. ncbi request reprint Neural plate morphogenesis during mouse neurulation is regulated by antagonism of Bmp signalling
    Patricia Ybot-Gonzalez
    Neural Development Unit, Institute of Child Health, University College London, 30 Guilford Street, London WC1N 1EH, UK
    Development 134:3203-11. 2007
    ..Our findings reveal a molecular mechanism based on antagonism of Bmp signalling that underlies the regulation of DLHP formation during mouse spinal neural tube closure...
  13. pmc Gene-environment interactions in the causation of neural tube defects: folate deficiency increases susceptibility conferred by loss of Pax3 function
    Katie A Burren
    Neural Development Unit, UCL Institute of Child Health, University College London, London, UK
    Hum Mol Genet 17:3675-85. 2008
    ..Hence, folate deficiency increases the risk of NTDs in genetically predisposed splotch embryos, probably via embryonic growth retardation...
  14. pmc Is LMNB1 a susceptibility gene for neural tube defects in humans?
    Alexis Robinson
    Neural Development Unit and Newlife Birth Defects Research Centre, Institute of Child Health, University College London, United Kingdom
    Birth Defects Res A Clin Mol Teratol 97:398-402. 2013
    ..In mice, polymorphism in Lmnb1 has been shown to modify risk of neural tube defects (NTDs), malformations of the central nervous system that result from incomplete closure of the neural folds...
  15. pmc Convergent extension, planar-cell-polarity signalling and initiation of mouse neural tube closure
    Patricia Ybot-Gonzalez
    Neural Development Unit, Institute of Child Health, University College London, 30 Guilford Street, London WC1N 1EH, UK
    Development 134:789-99. 2007
    ..These findings identify a cell-autonomous defect of convergent extension, requiring PCP signalling via RhoA-Rho kinase, during the development of severe neural tube defects in the mouse...
  16. pmc Nucleotide precursors prevent folic acid-resistant neural tube defects in the mouse
    Kit Yi Leung
    Neural Development Unit and Birth Defects Research Centre, Institute of Child Health, University College London, UK
    Brain 136:2836-41. 2013
    ..These findings may provide an impetus for future investigations of supplemental nucleotides as a means to prevent a greater proportion of human neural tube defects than can be achieved by folic acid alone. ..
  17. ncbi request reprint Integrity of the methylation cycle is essential for mammalian neural tube closure
    Louisa P E Dunlevy
    Neural Development Unit, Institute of Child Health, University College London, United Kingdom
    Birth Defects Res A Clin Mol Teratol 76:544-52. 2006
    ..Closure of the cranial neural tube during embryogenesis is a crucial process in development of the brain. Failure of this event results in the severe neural tube defect (NTD) exencephaly, the developmental forerunner of anencephaly...
  18. ncbi request reprint Increased expression of Grainyhead-like-3 rescues spina bifida in a folate-resistant mouse model
    Peter Gustavsson
    Neural Development Unit, Institute of Child Health, University College London, Guilford Street, London WC1N 1EH, UK
    Hum Mol Genet 16:2640-6. 2007
    ..This study provides evidence for a critical role of diminished Grhl3 expression in causing spinal NTDs in the curly tail mouse model...
  19. pmc Convergent extension analysis in mouse whole embryo culture
    Sophie E Pryor
    Neural Development Unit, Institute of Child Health, University College London, London, UK
    Methods Mol Biol 839:133-46. 2012
    ..We describe analytical methods that can reveal the abnormalities that characterise neurulation-stage embryos with defective planar cell polarity signalling, in particular the loop-tail (Lp; Vangl2) mutant...
  20. pmc In vivo genetic ablation by Cre-mediated expression of diphtheria toxin fragment A
    Anna Ivanova
    Neural Development Unit, Institute of Child Health, University College London, United Kingdom
    Genesis 43:129-35. 2005
    ..We observed loss of the precise tissues in which Nkx2.5 and Wnt1 are expressed. Apart from being a general GFP reporter, the ROSA26-GFP-DTA mouse line should provide a useful resource for genetic ablation of specific groups of cells...
  21. ncbi request reprint Specific isoforms of protein kinase C are essential for prevention of folate-resistant neural tube defects by inositol
    Patricia Cogram
    Neural Development Unit, Institute of Child Health, 30 Guilford Street, London WC1N 1EH, UK
    Hum Mol Genet 13:7-14. 2004
    ..Hindgut cell proliferation was stimulated specifically by inositol, an effect that required activation of PKCbetaI. Our findings reveal an essential role of specific PKC isoforms in mediating the prevention of mouse NTDs by inositol...
  22. pmc Folate metabolite profiling of different cell types and embryos suggests variation in folate one-carbon metabolism, including developmental changes in human embryonic brain
    Kit Yi Leung
    Neural Development Unit and Newlife Birth Defects Research Centre, Institute of Child Health, University College London, London, UK
    Mol Cell Biochem 378:229-36. 2013
    ..These cell type-specific and developmental changes in folate profile may indicate differential requirements for the various outputs of folate metabolism...
  23. doi request reprint Mutations in the planar cell polarity genes CELSR1 and SCRIB are associated with the severe neural tube defect craniorachischisis
    Alexis Robinson
    UCL Institute of Child Health, London WC1N 1EH, United Kingdom
    Hum Mutat 33:440-7. 2012
    ..We conclude that missense variants in CELSR1 and SCRIB may represent a cause of CRN in humans, as in mice, with defective PCP protein trafficking to the plasma membrane a likely pathogenic mechanism...
  24. pmc Neural tube defects--disorders of neurulation and related embryonic processes
    Andrew J Copp
    Neural Development Unit, Institute of Child Health, University College London, London, UK
    Wiley Interdiscip Rev Dev Biol 2:213-27. 2013
    ..Not all NTDs respond to FA, however, and adjunct therapies are required for prevention of this FA-resistant category...
  25. doi request reprint Protein deiminases: new players in the developmentally regulated loss of neural regenerative ability
    Sigrun Lange
    Developmental Biology Unit, UCL Institute of Child Health, London WC1N 1EH, UK
    Dev Biol 355:205-14. 2011
    ..Altogether, our findings identify PADs and deimination as developmentally regulated modulators of secondary injury response, and suggest that PADs might be valuable therapeutic targets for spinal cord injury...
  26. pmc Regional differences in the expression of laminin isoforms during mouse neural tube development
    Andrew J Copp
    Neural Development Unit, Institute of Child Health, University College London, UK
    Matrix Biol 30:301-9. 2011
    ..This information paves the way towards a mechanistic analysis of basement membrane laminin function during early neural tube development in mammals...
  27. pmc Apoptosis is not required for mammalian neural tube closure
    Valentina Massa
    Neural Development Unit, University College London Institute of Child Health, London, United Kingdom
    Proc Natl Acad Sci U S A 106:8233-8. 2009
    ..We conclude that apoptosis is not required for neural tube closure in the mouse embryo...
  28. ncbi request reprint Post-translational regulation of Crmp in developing and regenerating chick spinal cord
    Stefanie Gögel
    Developmental Biology Unit, UCL Institute of Child Health, London, United Kingdom
    Dev Neurobiol 70:456-71. 2010
    ....
  29. ncbi request reprint The genetic basis of mammalian neurulation
    Andrew J Copp
    Neural Development Unit, Institute of Child Health, University College London, London WC1N 1EH, UK
    Nat Rev Genet 4:784-93. 2003
    ..Mutant mice also offer an opportunity to unravel the mechanisms by which folic acid prevents neural tube defects, and to develop new therapies for folate-resistant defects...
  30. ncbi request reprint Excess methionine suppresses the methylation cycle and inhibits neural tube closure in mouse embryos
    Louisa P E Dunlevy
    Neural Development Unit, Institute of Child Health, University College London, 30 Guilford Street, London WC1N 1EA, UK
    FEBS Lett 580:2803-7. 2006
    ..Thus, reduced cranial mesenchyme density and impairment of critical methylation reactions may contribute to development of methionine-induced NTD...
  31. pmc Neural tube defects: recent advances, unsolved questions, and controversies
    Andrew J Copp
    Neural Development Unit and Newlife Birth Defects Research Centre, UCL Institute of Child Health, London, UK
    Lancet Neurol 12:799-810. 2013
    ..Other malformations, such as encephalocele, are likely to be postneurulation disorders. ..
  32. pmc Quantitative analysis of myo-inositol in urine, blood and nutritional supplements by high-performance liquid chromatography tandem mass spectrometry
    Kit Yi Leung
    Neural Development Unit, Institute of Child Health, University College London, 30 Guilford Street, London, WC1N 1EH, United Kingdom
    J Chromatogr B Analyt Technol Biomed Life Sci 879:2759-63. 2011
    ..1% and 3.5% respectively, while intra-assay CV was 2.3% and 3.6%. Urine and blood samples from normal individuals were analysed...
  33. pmc Vangl-dependent planar cell polarity signalling is not required for neural crest migration in mammals
    Sophie E Pryor
    Newlife Birth Defects Research Centre, Institute of Child Health, University College London, 30 Guilford Street, London, WC1N 1EH, UK
    Development 141:3153-8. 2014
    ..Hence, PCP signalling is not essential for NC migration in mammals, in contrast to its essential role in neural tube closure. PCP mutations are thus unlikely to mediate NC-related birth defects in humans. ..
  34. ncbi request reprint Zic4, a zinc-finger transcription factor, is expressed in the developing mouse nervous system
    Carles Gaston-Massuet
    Neural Development Unit, Institute of Child Health, University College London, United Kingdom
    Dev Dyn 233:1110-5. 2005
    ..We conclude that Zic4 has an expression pattern distinct from, but partly overlapping with, other members of the Zic gene family...
  35. ncbi request reprint A coming of age: advanced imaging technologies for characterising the developing mouse
    Francesca C Norris
    University College London UCL Centre for Advanced Biomedical Imaging, Division of Medicine, UCL, London, UK Centre for Mathematics and Physics in the Life Sciences and Experimental Biology CoMPLEX, UCL, London, UK
    Trends Genet 29:700-11. 2013
    ....
  36. pmc Metformin retards aging in C. elegans by altering microbial folate and methionine metabolism
    Filipe Cabreiro
    Institute of Healthy Ageing, and G E E, University College London, London WC1E 6BT, UK
    Cell 153:228-39. 2013
    ..Thus, metformin-induced alteration of microbial metabolism could contribute to therapeutic efficacy-and also to its side effects, which include folate deficiency and gastrointestinal upset...
  37. pmc Genetics of human neural tube defects
    Nicholas D E Greene
    Neural Development Unit, UCL Institute of Child Health, London, UK
    Hum Mol Genet 18:R113-29. 2009
    ....
  38. pmc Genetics and development of neural tube defects
    Andrew J Copp
    Neural Development Unit, UCL Institute of Child Health, London WC1N 1EH, UK
    J Pathol 220:217-30. 2010
    ..Folic acid-resistant cases can be prevented by inositol supplementation in mice, raising the possibility that this could lead to an additional preventive strategy for human NTDs in future...
  39. ncbi request reprint D-chiro-inositol is more effective than myo-inositol in preventing folate-resistant mouse neural tube defects
    Patricia Cogram
    Neural Development Unit, Institute of Child Health, University College London, UK
    Hum Reprod 17:2451-8. 2002
    ..Here, the effectiveness and safety during pregnancy of two isomers, myo- and D-chiro-inositol, in preventing mouse NTDs was compared...
  40. ncbi request reprint Expression pattern of glypican-4 suggests multiple roles during mouse development
    Patricia Ybot-Gonzalez
    Neural Development Unit, Institute of Child Health, University College London, United Kingdom
    Dev Dyn 233:1013-7. 2005
    ..Glypican-4 expression is not detected in the midline of the embryo at the stage of initiation of neural tube closure, suggesting that glypican-4 is unlikely to play an essential role in convergent extension in the mouse...
  41. ncbi request reprint Quantitative analysis of s-adenosylmethionine and s-adenosylhomocysteine in neurulation-stage mouse embryos by liquid chromatography tandem mass spectrometry
    Katie A Burren
    Neural Development Unit, Institute of Child Health, University College London, 30 Guilford Street, London WC1N 1EH, United Kingdom
    J Chromatogr B Analyt Technol Biomed Life Sci 844:112-8. 2006
    ..Calibration curves suitable for the analysis of neurulation-stage mouse embryos (SAM 0.02-25.0microM, SAH 0.01-10.0microM) were linear (r(2)>0.997) with limits of detection for SAM and SAH of 10 and 2.5nmol/L, respectively...
  42. doi request reprint Defining a PARticular pathway of neural tube closure
    Andrew J Copp
    UCL Institute of Child Health, 30 Guilford Street, London WC1N 1EH, UK
    Dev Cell 18:1-2. 2010
    ....
  43. ncbi request reprint Dishevelled: linking convergent extension with neural tube closure
    Andrew J Copp
    Neural Development Unit, Institute of Child Health, University College, London WC1N 1EH, UK
    Trends Neurosci 26:453-5. 2003
    ..Although bilateral neural folds form, they are abnormally far apart and cannot achieve the apposition necessary for neural tube closure...
  44. doi request reprint EphrinA-EphA receptor interactions in mouse spinal neurulation: implications for neural fold fusion
    Noraishah M Abdul-Aziz
    Neural Development Unit, UCL Institute of Child Health, London WC1N 1EH, UK
    Int J Dev Biol 53:559-68. 2009
    ....
  45. doi request reprint Magnetic resonance virtual histology for embryos: 3D atlases for automated high-throughput phenotyping
    Jon O Cleary
    Centre for Advanced Biomedical Imaging, Department of Medicine, University College London, London, UK
    Neuroimage 54:769-78. 2011
    ..Our work demonstrates the feasibility of combining high-throughput embryo MRI with automated analysis techniques to distinguish novel mouse phenotypes...
  46. pmc The emerging role of epigenetic mechanisms in the etiology of neural tube defects
    Nicholas D E Greene
    Neural Development Unit, Institute of Child Health, UCL, London, UK
    Epigenetics 6:875-83. 2011
    ..Alterations in the epigenetic status can be directly modified by various environmental insults or maternal dietary factors...
  47. ncbi request reprint Neural tube defects
    Nicholas D E Greene
    Newlife Birth Defects Research Center, Institute of Child Health, University College London, WC1N 1EH, United Kingdom email
    Annu Rev Neurosci 37:221-42. 2014
    ..Such models also provide an opportunity to investigate potential risk factors and to develop novel preventive therapies. ..
  48. ncbi request reprint Planar cell polarity genes and neural tube closure
    Naoto Ueno
    Department of Developmental Biology, National Institute for Basic Biology, and SOKENDAI, Okazaki, Japan
    Birth Defects Res C Embryo Today 69:318-24. 2003
    ..Therefore, understanding the mechanisms underlying the establishment of cell polarity in Drosophila may provide important clues to the molecular basis of NTDs...
  49. ncbi request reprint Folic acid prevents exencephaly in Cited2 deficient mice
    Juan Pedro Martinez Barbera
    MRC Centre for Developmental Neurobiology, 4th floor New Hunt s House, King s College London, Guy s Campus, London Bridge, London SE1 1UL, UK
    Hum Mol Genet 11:283-93. 2002
    ..To our knowledge, the Cited2 mouse represents the first genetic model in which folic acid can prevent a defect in neural tube closure by a mechanism other than the neutralization of a defect in folate homeostasis...
  50. ncbi request reprint Grainyhead genes and mammalian neural tube closure
    Peter Gustavsson
    Department of Molecular Medicine and Surgery, Karolinska Institute, Stockholm, Sweden
    Birth Defects Res A Clin Mol Teratol 82:728-35. 2008
    ..However, the developmental and cellular basis of NTDs in curly tail mutants is well established, involving a proliferation defect in the hindgut endoderm...
  51. ncbi request reprint Retinal pathology and function in a Cln3 knockout mouse model of juvenile Neuronal Ceroid Lipofuscinosis (batten disease)
    Gail M Seigel
    Department of Ophthalmology, University of Rochester School of Medicine, New York, USA
    Mol Cell Neurosci 19:515-27. 2002
    ....