Research Topics
| M Helen GrantSummaryAffiliation: University of Strathclyde Country: UK Publications
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Detail Information
Publications
The effect of serum from liver cancer patients on the growth and function of primary and immortalised hepatocytesM H Grant
Bioengineering Unit, University of Strathclyde, Glasgow, Scotland
Int J Artif Organs 24:807-13. 2001..The serum may have to be pretreated with adsorbants to remove toxins prior to BAL treatment...- The viability and function of primary rat hepatocytes cultured on polymeric membranes developed for hybrid artificial liver devicesM Helen Grant
Bioengineering Unit, Strathclyde University, Wolfson Centre, Glasgow, UK
J Biomed Mater Res A 73:367-75. 2005..Improving the chemistry of membranes for artificial liver devices will enhance the phenotypic stability of the cells, enabling us to prolong treatment times for patients... 
Inactivation of microorganisms within collagen gel biomatrices using pulsed electric field treatmentSarah Griffiths
Bioengineering Unit, University of Strathclyde, Wolfson Centre, Glasgow, UK
J Mater Sci Mater Med 23:507-15. 2012..Further investigations are required to optimise the microbial inactivation kinetics associated with PEF treatment of collagen gel biomatrices...
Acute inflammatory response to cobalt chromium orthopaedic wear debris in a rodent air-pouch modelMoeed Akbar
Bioengineering Unit, University of Strathclyde, Wolfson Centre, Glasgow, UK
J R Soc Interface 9:2109-19. 2012..The data suggest that inflammatory responses to CoCr debris induce a specific acute process in which the recruitment of monocytes/macrophages is key...- Comparative chronic in vitro toxicity of hexavalent chromium to osteoblasts and monocytesVijay Krishna Raghunathan
Bioengineering Unit, Wolfson Centre, University of Strathclyde, Glasgow, Scotland
J Biomed Mater Res A 88:543-50. 2009..Data suggest that both cell types mount an adaptive response to chronic exposure to Cr VI, but this is more potent in osteoblasts, and results in the relative resilience of this cell type to the effects of Cr VI on cell viability... - Distribution of metal released from cobalt-chromium alloy orthopaedic wear particles implanted into air pouches in miceGrace A Afolaranmi
Bioengineering Unit, University of Strathclyde, Wolfson Center, Glasgow G4 0NW, United Kingdom
J Biomed Mater Res A 100:1529-38. 2012.... - Changes in protein expression associated with chronic in vitro exposure of hexavalent chromium to osteoblasts and monocytes: a proteomic approachVijay Krishna Raghunathan
Bioengineering Unit, Wolfson Centre, 106 Rottenrow, University of Strathclyde, Glasgow, G4 0NW, United Kingdom
J Biomed Mater Res A 92:615-25. 2010..The results highlight the complex molecular changes that occur in both cell types with long-term exposure to Cr and may be useful in establishing a series of clinically useful biomarkers to monitor long-term use of metallic implants... - Effect of chromium and cobalt ions on phase I and phase II enzymatic activities in vitro in freshly isolated rat hepatocytesGrace A Afolaranmi
Bioengineering Unit, University of Strathclyde, Wolfson Centre, Glasgow, United Kingdom
Toxicol In Vitro 25:125-30. 2011..High concentrations of these metal ions in the livers of patients with loose or worn metal implants may act synergistically, and have consequences for the metabolism of xenobiotics... - Metabolic studies of hepatocytes cultured on collagen substrata modified to contain glycosaminoglycansMargarita Kataropoulou
Bioengineering Unit, University of Strathclyde, Glasgow, UK
Tissue Eng 11:1263-73. 2005..This approach may increase the expression of hepatocyte-specific functions in monolayers cultured on membranes in flat plate bioreactors... - Effect of chromium and cobalt ions on primary human lymphocytes in vitroMoeed Akbar
Bioengineering Unit, University of Strathclyde, Wolfson Centre, Glasgow G4 0NW, UK
J Immunotoxicol 8:140-9. 2011..Hence, this may contribute to altered immune system function in patients with Co-Cr implants... - The effect of betacyclodextrin and hydroxypropyl betacyclodextrin incorporation into plasticized poly(vinyl chloride) on its compatibility with human U937 cellsSusan M George
Bioengineering Unit, Wolfson Centre, Strathclyde University, 106 Rottenrow, Glasgow, G4 0NW UK
J Biomed Mater Res B Appl Biomater 96:310-5. 2011..However, the HPBCD modified plasticized PVC materials caused a reduction in the production in TNF-α induced by LPS which may have implications for the inflammatory potential of these biomaterials... - The effect of anticoagulants on the distribution of chromium VI in blood fractionsGrace A Afolaranmi
Strathclyde Institute of Pharmacy and Biomedical Sciences, University of Strathclyde, Glasgow, UK
J Arthroplasty 25:118-20. 2010..05). The extent of accumulation in red blood cells is influenced by the anticoagulant used to collect the blood, with EDTA giving a lower partitioning into red cells compared with sodium citrate and sodium heparin... - Effect of 405-nm high-intensity narrow-spectrum light on fibroblast-populated collagen lattices: an in vitro model of wound healingRichard McDonald
University of Strathclyde, Bioengineering Unit, Wolfson Centre, 106 Rottenrow, Glasgow, Strathclyde G4 0NW, United Kingdom
J Biomed Opt 16:048003. 2011..These results show that HINS light has potential for disinfection applications without adversely influencing wound healing... - Metabolism of two new benzodiazepine-type anti-leishmanial agents in rat hepatocytes and hepatic microsomes and their interaction with glutathione in macrophagesM Dao Duong Thi
Strathclyde Institute of Pharmacy and Biomedical Sciences, University of Strathclyde, Glasgow, UK
J Pharm Pharmacol 61:399-406. 2009.... - Cr (VI) inhibits DNA, RNA and protein syntheses in hepatocytes: involvement of glutathione reductase, reduced glutathione and DT-diaphoraseMekala Gunaratnam
Bioengineering Unit, University of Strathclyde, Wolfson Centre, Glasgow G4 0NW, UK
Toxicol In Vitro 22:879-86. 2008..The concentrations of Cr (VI) found to inhibit macromolecular syntheses in this study are clinically relevant: it is therefore important to develop implants with minimum wear potential... - The toxicity of opiates and their metabolites in HepG2 cellsMark Jairaj
Department of Pharmaceutical Sciences, John Arbuthnott Building, University of Strathclyde, Glasgow G4 0NR, UK
Chem Biol Interact 146:121-9. 2003.... - Elucidation of the Phase I and Phase II metabolic pathways of (±)-4'-methylmethcathinone (4-MMC) and (±)-4'-(trifluoromethyl)methcathinone (4-TFMMC) in rat liver hepatocytes using LC-MS and LC-MS(2)Osama I G Khreit
Strathclyde Institute of Pharmacy and Biomedical Sciences SIPBS, University of Strathclyde, 161 Cathedral Street, Glasgow G4 0RE, UK
J Pharm Biomed Anal 72:177-85. 2013..9min and for 4-TFMMC=203.8min] and this data may aid in the understanding of in vivo metabolism and the likely pharmacokinetic effects of chemical/structural modifications within this class of controlled substances... - Pulsed electric field as a potential new method for microbial inactivation in scaffold materials for tissue engineering: the effect on collagen as a scaffoldSharon Smith
Bioengineering Unit, University of Strathclyde, Wolfson Centre, 106 Rottenrow, Glasgow G40NW, United Kingdom
J Biomed Mater Res A 90:844-51. 2009..PEF treatment, therefore, shows great potential to be used as a sterilization method for collagen-based biomaterials... - The mechanical strength of collagen gels containing glycosaminoglycans and populated with fibroblastsZiad A Saddiq
University of Strathclyde, Wolfson Centre, Glasgow, UK
J Biomed Mater Res A 89:697-706. 2009..With most parameters, the effect of the cells was independent of gel composition, and the presence of crosslinkers or GAG did not impart resistance to the cell-induced decrease in strength... - The effect of ascorbic acid on the distribution of soluble Cr and Co ions in the blood and organs of ratsGrace A Afolaranmi
Bioengineering Unit, University of Strathclyde, Wolfson Centre, Glasgow, UK
J Appl Toxicol 33:220-6. 2013..AA may only be effective in increasing elimination of Cr VI at high concentrations when plasma reduction is saturated, and may be of limited therapeutic use in patients with orthopaedic implants... - Confined compression of collagen hydrogelsGrahame A Busby
Department of Biomedical Engineering, University of Strathclyde, Wolfson Centre, 106 Rottenrow, Glasgow G4 0NW, UK
J Biomech 46:837-40. 2013..This study suggests that confined compression, together with biphasic theory, is a suitable technique for assessing the mechanical properties of collagen hydrogels... - Response to chronic exposure to hexavalent chromium in human monocytesVijay Krishna Raghunathan
Bioengineering Unit, Wolfson Centre, University of Strathclyde, Glasgow G4 0NW, United Kingdom
Toxicol In Vitro 23:647-52. 2009..Such direct toxicity of Cr ions may contribute to the effects of metal implants on lymphocyte populations in vivo... - The role of glutathione reductase in the cytotoxicity of chromium (VI) in isolated rat hepatocytesM Gunaratnam
Bioengineering Unit, Strathclyde University, Wolfson Centre, 106 Rottenrow, G4 0NW, Glasgow, UK
Chem Biol Interact 134:191-202. 2001..Glutathione reductase appears to play an important role in the enzymatic reduction of Cr(VI) as inhibition of this enzyme by carmustine (BCNU) markedly protected the cells from cytotoxicity... - Metabolism of troglitazone in hepatocytes isolated from experimentally induced diabetic ratsA J Meechan
Department of Pharmaceutical Sciences, University of Strathclyde, 27 Taylor Street, Glasgow G4 0NR, Scotland, UK
J Pharm Pharmacol 58:1359-65. 2006.... - Cryopreservation of rat hepatocyte monolayers: cell viability and cytochrome P450 content in post-thaw culturesG C McKay
Bioengineering Unit, Strathclyde University, Wolfson Centre, Glasgow G4 0NW, UK
Toxicol In Vitro 16:71-9. 2002..Cytochrome P450 content was retained in post-thaw culture to a similar extent as in non-frozen cultures. Cryopreserved rat hepatocyte monolayers may provide a useful in vitro model for studying xenobiotic metabolism and toxicity... - Pretreatment of rats with the inducing agents phenobarbitone and 3-methylcholanthrene ameliorates the toxicity of chromium (VI) in hepatocytesM Gunaratnam
Bioengineering Unit, University of Strathclyde, Wolfson Centre, 106 Rottenrow, Glasgow G4 0NW, Scotland, UK
Toxicol In Vitro 16:509-16. 2002..Exposure to environmental inducing agents in vivo may also alter the response of human tissues to Cr(VI)... - Measurement of the intracellular distribution of reduced glutathione in cultured rat hepatocytes using monochlorobimane and confocal laser scanning microscopyD Stevenson
Bioengineering Unit, University of Strathclyde, Wolfson Centre, 106 Rottenrow, Glasgow G4 0NW, Scotland, UK
Toxicol In Vitro 16:609-19. 2002..The combination of a specific stain, a novel laser light source and confocal microscopy provide a valuable system for mechanistic studies of intracellular GSH distribution in toxicology studies... - The effect of inducing agents on the metabolism of trypanocidal diamidines by isolated rat hepatocytesC Atsriku
Department of Pharmaceutical Sciences, University of Strathclyde, 27 Taylor Street, Glasgow, G4 0NR, UK
Chem Biol Interact 146:297-305. 2003.... - Cryopreservation of viable hepatocyte monolayers in cryoprotectant media with high serum content: metabolism of testosterone and kaempherol post-cryopreservationD J Stevenson
Bioengineering Unit, University of Strathclyde, Wolfson Centre, 106 Rottenrow, Glasgow G4 0NW, UK
Cryobiology 49:97-113. 2004..This method of cryopreservation may provide a simple, convenient means of long-term storage of hepatocytes for in vitro metabolism studies... - The interaction of chromium (VI) with macrophages: depletion of glutathione and inhibition of glutathione reductaseA Lalaouni
Bioengineering Unit, University of Strathclyde, Wolfson Centre, Glasgow G4 0NW, United Kingdom
Toxicology 236:76-81. 2007..Prior inhibition of glutathione reductase activity protects against the toxicity of Cr VI to a significant extent, suggesting that it reduces Cr VI to a toxic metabolite... - The role of reduced glutathione and glutathione reductase in the cytotoxicity of chromium (VI) in osteoblastsJ Ning
Bioengineering Unit, Strathclyde University, Wolfson Centre, G4 0NW, Glasgow, UK
Toxicol In Vitro 14:329-35. 2000..The results indicate that glutathione reductase plays an important role in the intracellular reduction of Cr VI in osteoblasts...