Berthold Gottgens

Summary

Affiliation: University of Cambridge
Country: UK

Publications

  1. pmc The transcriptional programme controlled by Runx1 during early embryonic blood development
    Yosuke Tanaka
    Laboratory for Stem Cell Biology, RIKEN Center for Developmental Biology, Kobe, Japan
    Dev Biol 366:404-19. 2012
  2. pmc Esrrb is a pivotal target of the Gsk3/Tcf3 axis regulating embryonic stem cell self-renewal
    Graziano Martello
    Wellcome Trust Medical Research Council Stem Cell Institute, University of Cambridge, Cambridge UK
    Cell Stem Cell 11:491-504. 2012
  3. pmc A new RNASeq-based reference transcriptome for sugar beet and its application in transcriptome-scale analysis of vernalization and gibberellin responses
    Effie S Mutasa-Göttgens
    Rothamsted Research Broom s Barn, Department of Applied Crop Science, Higham, Bury St Edmunds, Suffolk IP26 6NP, UK
    BMC Genomics 13:99. 2012
  4. pmc Fast and sensitive multiple alignment of large genomic sequences
    Michael Brudno
    Department of Computer Science, Stanford University, Stanford, CA 94305, USA
    BMC Bioinformatics 4:66. 2003
  5. pmc Establishing the transcriptional programme for blood: the SCL stem cell enhancer is regulated by a multiprotein complex containing Ets and GATA factors
    Berthold Gottgens
    University of Cambridge Department of Haematology, Cambridge Institute for Medical Research, Hills Road, Cambridge CB2 2XY, UK
    EMBO J 21:3039-50. 2002
  6. ncbi Transcriptional regulation of haematopoiesis
    B Gottgens
    Department of Haematology, Cambridge University, Cambridge Institute for Medical Research, Hills Road, Cambridge CB2 2XY, UK
    Vox Sang 87:15-9. 2004
  7. pmc The scl +18/19 stem cell enhancer is not required for hematopoiesis: identification of a 5' bifunctional hematopoietic-endothelial enhancer bound by Fli-1 and Elf-1
    Berthold Gottgens
    Department of Hematology, Cambridge Institute for Medical Research, University of Cambridge, United Kingdom
    Mol Cell Biol 24:1870-83. 2004
  8. pmc Transcriptional regulation of the stem cell leukemia gene (SCL)--comparative analysis of five vertebrate SCL loci
    Berthold Gottgens
    Cambridge Institute for Medical Research, Cambridge University, Cambridge, CB2 2XY, United Kingdom
    Genome Res 12:749-59. 2002
  9. pmc Long-range comparison of human and mouse SCL loci: localized regions of sensitivity to restriction endonucleases correspond precisely with peaks of conserved noncoding sequences
    B Gottgens
    The Wellcome Trust Centre for Molecular Mechanisms in Disease, Cambridge Institute for Medical Research, Addenbrooke s Hospital Site, Cambridge CB2 2XY, UK
    Genome Res 11:87-97. 2001
  10. ncbi Fli1, Elf1, and Ets1 regulate the proximal promoter of the LMO2 gene in endothelial cells
    Josette Renee Landry
    Department of Haematology, Cambridge Institute for Medical Research, Cambridge University, Hills Rd, Cambridge, CB2 2XY, United Kingdom
    Blood 106:2680-7. 2005

Detail Information

Publications74

  1. pmc The transcriptional programme controlled by Runx1 during early embryonic blood development
    Yosuke Tanaka
    Laboratory for Stem Cell Biology, RIKEN Center for Developmental Biology, Kobe, Japan
    Dev Biol 366:404-19. 2012
    ..Moreover, the concept of interplay between sequentially deployed core regulators is likely to represent a design principle widely applicable to the transcriptional control of mammalian development...
  2. pmc Esrrb is a pivotal target of the Gsk3/Tcf3 axis regulating embryonic stem cell self-renewal
    Graziano Martello
    Wellcome Trust Medical Research Council Stem Cell Institute, University of Cambridge, Cambridge UK
    Cell Stem Cell 11:491-504. 2012
    ..These findings highlight a key role for Esrrb in regulating the naive pluripotent state and illustrate compensation among the core pluripotency factors...
  3. pmc A new RNASeq-based reference transcriptome for sugar beet and its application in transcriptome-scale analysis of vernalization and gibberellin responses
    Effie S Mutasa-Göttgens
    Rothamsted Research Broom s Barn, Department of Applied Crop Science, Higham, Bury St Edmunds, Suffolk IP26 6NP, UK
    BMC Genomics 13:99. 2012
    ..In this study, we demonstrate the application of an mRNA-Seq based strategy for this purpose...
  4. pmc Fast and sensitive multiple alignment of large genomic sequences
    Michael Brudno
    Department of Computer Science, Stanford University, Stanford, CA 94305, USA
    BMC Bioinformatics 4:66. 2003
    ..In a first step, a fast search program identifies a chain of strong local sequence similarities. In a second step, regions between these anchor points are aligned using a slower but more accurate method...
  5. pmc Establishing the transcriptional programme for blood: the SCL stem cell enhancer is regulated by a multiprotein complex containing Ets and GATA factors
    Berthold Gottgens
    University of Cambridge Department of Haematology, Cambridge Institute for Medical Research, Hills Road, Cambridge CB2 2XY, UK
    EMBO J 21:3039-50. 2002
    ..Our results suggest that these transcription factors are key components of an enhanceosome responsible for activating SCL transcription and establishing the transcriptional programme required for HSC formation...
  6. ncbi Transcriptional regulation of haematopoiesis
    B Gottgens
    Department of Haematology, Cambridge University, Cambridge Institute for Medical Research, Hills Road, Cambridge CB2 2XY, UK
    Vox Sang 87:15-9. 2004
  7. pmc The scl +18/19 stem cell enhancer is not required for hematopoiesis: identification of a 5' bifunctional hematopoietic-endothelial enhancer bound by Fli-1 and Elf-1
    Berthold Gottgens
    Department of Hematology, Cambridge Institute for Medical Research, University of Cambridge, United Kingdom
    Mol Cell Biol 24:1870-83. 2004
    ....
  8. pmc Transcriptional regulation of the stem cell leukemia gene (SCL)--comparative analysis of five vertebrate SCL loci
    Berthold Gottgens
    Cambridge Institute for Medical Research, Cambridge University, Cambridge, CB2 2XY, United Kingdom
    Genome Res 12:749-59. 2002
    ..Analysis of the SCL promoter/enhancer revealed five motifs, which were conserved from zebrafish to mammals, and each of which is essential for the appropriate pattern or level of SCL transcription...
  9. pmc Long-range comparison of human and mouse SCL loci: localized regions of sensitivity to restriction endonucleases correspond precisely with peaks of conserved noncoding sequences
    B Gottgens
    The Wellcome Trust Centre for Molecular Mechanisms in Disease, Cambridge Institute for Medical Research, Addenbrooke s Hospital Site, Cambridge CB2 2XY, UK
    Genome Res 11:87-97. 2001
    ....
  10. ncbi Fli1, Elf1, and Ets1 regulate the proximal promoter of the LMO2 gene in endothelial cells
    Josette Renee Landry
    Department of Haematology, Cambridge Institute for Medical Research, Cambridge University, Hills Rd, Cambridge, CB2 2XY, United Kingdom
    Blood 106:2680-7. 2005
    ..Together, these results suggest that the conserved proximal promoter is central to LMO2 transcription in hematopoietic and endothelial cells, where it is regulated by Ets factors...
  11. ncbi Tdr2, a new zebrafish transposon of the Tc1 family
    B Gottgens
    Department of Haematology, Cambridge University, MRC Centre, Hills Road, Cambridge, UK
    Gene 239:373-9. 1999
    ..Tdr2 transposons may facilitate the development of novel transposon-based tools for the genetic analysis of zebrafish...
  12. pmc Transcriptional regulation of haematopoietic transcription factors
    Nicola K Wilson
    University of Cambridge Department of Haematology, Cambridge Institute for Medical Research, Hills Road, Cambridge, CB2 0XY, UK
    Stem Cell Res Ther 2:6. 2011
    ..We also explore how changes in technology will impact on this area of research in the future...
  13. pmc Integration of Elf-4 into stem/progenitor and erythroid regulatory networks through locus-wide chromatin studies coupled with in vivo functional validation
    Aileen M Smith
    University of Cambridge Department of Haematology, Cambridge Institute for Medical Research, Cambridge, United Kingdom
    Mol Cell Biol 32:763-73. 2012
    ....
  14. pmc Transcriptional regulation of Elf-1: locus-wide analysis reveals four distinct promoters, a tissue-specific enhancer, control by PU.1 and the importance of Elf-1 downregulation for erythroid maturation
    Fernando J Calero-Nieto
    Department of Haematology, Cambridge Institute for Medical Research, Cambridge University, Hills Road, Cambridge CB2 0XY, UK
    Nucleic Acids Res 38:6363-74. 2010
    ..1 controls myeloid-erythroid differentiation. Our findings therefore not only represent the first report of Elf-1 regulation but also enhance our understanding of the wider regulatory networks that control haematopoiesis...
  15. pmc The SCL transcriptional network and BMP signaling pathway interact to regulate RUNX1 activity
    John E Pimanda
    Cambridge Institute for Medical Research, University of Cambridge, Cambridge CB2 2XY, United Kingdom
    Proc Natl Acad Sci U S A 104:840-5. 2007
    ..Taken together, our results integrate three key determinants of HSC development; the Scl transcriptional network, Runx1 activity, and the Bmp4/Smad signaling pathway...
  16. doi The transcriptional program controlled by the stem cell leukemia gene Scl/Tal1 during early embryonic hematopoietic development
    Nicola K Wilson
    University of Cambridge, Department of Haematology, Cambridge Institute for Medical Research, Cambridge, United Kingdom
    Blood 113:5456-65. 2009
    ....
  17. doi Runx genes are direct targets of Scl/Tal1 in the yolk sac and fetal liver
    Josette Renee Landry
    Department of Haematology, Cambridge Institute for Medical Research, Cambridge University, Cambridge, United Kingdom
    Blood 111:3005-14. 2008
    ..Together, our data provide a key component of the transcriptional network of early hematopoiesis by identifying downstream targets of Scl that can explain key aspects of the early Scl(-/-) phenotype...
  18. pmc Endoglin expression in blood and endothelium is differentially regulated by modular assembly of the Ets/Gata hemangioblast code
    John E Pimanda
    Department of Haematology, Cambridge Institute for Medical Research, University of Cambridge, Cambridge, United Kingdom
    Blood 112:4512-22. 2008
    ..This modular assembly of regulators gives blood and endothelial cells the regulatory freedom to independently fine-tune gene expression and emphasizes the role of regulatory divergence in driving functional divergence...
  19. ncbi Genome-wide identification of cis-regulatory sequences controlling blood and endothelial development
    Ian J Donaldson
    Department of Haematology, Cambridge Institue for Medical Research, Cambridge University, UK
    Hum Mol Genet 14:595-601. 2005
    ....
  20. ncbi Transgenic analysis of the stem cell leukemia +19 stem cell enhancer in adult and embryonic hematopoietic and endothelial cells
    Lev Silberstein
    Department of Hematology, Cambridge Institute for Medical Research, Cambridge University, Hills Road, Cambridge CB2 2XY, UK
    Stem Cells 23:1378-88. 2005
    ..Moreover, activity in a proportion of thymocytes and other SCL-negative cell types suggests the existence of a silencer elsewhere in the SCL locus...
  21. pmc Mapping and functional characterisation of a CTCF-dependent insulator element at the 3' border of the murine Scl transcriptional domain
    George A Follows
    Department of Haematology, Cambridge Institute for Medical Research, University of Cambridge, Cambridge, United Kingdom
    PLoS ONE 7:e31484. 2012
    ....
  22. pmc The transcriptional coactivator Cbp regulates self-renewal and differentiation in adult hematopoietic stem cells
    Wai In Chan
    Department of Haematology, University of Cambridge, Cambridge Institute for Medical Research, Cambridge CB2 0XY, United Kingdom
    Mol Cell Biol 31:5046-60. 2011
    ..Our data demonstrate that Cbp plays a role in adult HSC homeostasis by maintaining the balance between different HSC fate decisions, and our findings identify a putative HSC-specific transcriptional network coordinated by Cbp...
  23. ncbi The paralogous hematopoietic regulators Lyl1 and Scl are coregulated by Ets and GATA factors, but Lyl1 cannot rescue the early Scl-/- phenotype
    Wan Y I Chan
    Department of Haematology, Cambridge Institute for Medical Research, University of Cambridge, United Kingdom
    Blood 109:1908-16. 2007
    ..Furthermore, coregulation of Scl and Lyl1 later during development may explain the mild phenotype of Scl-/- adult HSCs...
  24. ncbi TFBScluster: a resource for the characterization of transcriptional regulatory networks
    Ian John Donaldson
    Department of Haematology, Cambridge Institute for Medical Research, University of Cambridge Hills Road, Cambridge CB2 2XY, UK
    Bioinformatics 21:3058-9. 2005
    ..TFBScluster, therefore, has the potential to contribute to deciphering transcriptional networks that regulate a wide range of mammalian developmental processes...
  25. pmc Characterization of transcriptional networks in blood stem and progenitor cells using high-throughput single-cell gene expression analysis
    Victoria Moignard
    Department of Haematology, Wellcome Trust and MRC Cambridge Stem Cell Institute and Cambridge Institute for Medical Research, University of Cambridge, Cambridge CB2 0XY, UK
    Nat Cell Biol 15:363-72. 2013
    ....
  26. pmc Gata2, Fli1, and Scl form a recursively wired gene-regulatory circuit during early hematopoietic development
    John E Pimanda
    Department of Haematology, Cambridge Institute for Medical Research, University of Cambridge, Cambridge CB2 0XY, United Kingdom
    Proc Natl Acad Sci U S A 104:17692-7. 2007
    ....
  27. pmc Gfi1 expression is controlled by five distinct regulatory regions spread over 100 kilobases, with Scl/Tal1, Gata2, PU.1, Erg, Meis1, and Runx1 acting as upstream regulators in early hematopoietic cells
    Nicola K Wilson
    University of Cambridge, Department of Haematology, Cambridge Institute for Medical Research, Cambridge, United Kingdom
    Mol Cell Biol 30:3853-63. 2010
    ....
  28. pmc Analysis of multiple genomic sequence alignments: a web resource, online tools, and lessons learned from analysis of mammalian SCL loci
    Michael A Chapman
    Cambridge Institute for Medical Research, Cambridge, CB2 2XY, UK
    Genome Res 14:313-8. 2004
    ..In the search for individual transcription factor binding sites, multiple alignments markedly increase the signal-to-noise ratio compared to pairwise alignments...
  29. ncbi Comparative and functional analyses of LYL1 loci establish marsupial sequences as a model for phylogenetic footprinting
    Michael A Chapman
    Department of Haematology, Cambridge Institute for Medical Research, Cambridge University, Hills Road, Cambridge CB2 2XY, UK
    Genomics 81:249-59. 2003
    ..This study represents the first large-scale comparative analysis involving marsupial genomic sequence and demonstrates that such comparisons provide a powerful approach to characterizing mammalian regulatory elements...
  30. doi Combinatorial transcriptional control in blood stem/progenitor cells: genome-wide analysis of ten major transcriptional regulators
    Nicola K Wilson
    University of Cambridge Department of Haematology, Cambridge Institute for Medical Research, Hills Road, Cambridge, CB2 0XY, UK
    Cell Stem Cell 7:532-44. 2010
    ..Taken together, this study demonstrates the power of genome-wide analysis in generating novel functional insights into the transcriptional control of stem and progenitor cells...
  31. doi Expression of the leukemia oncogene Lmo2 is controlled by an array of tissue-specific elements dispersed over 100 kb and bound by Tal1/Lmo2, Ets, and Gata factors
    Josette Renee Landry
    Department of Haematology, Cambridge Institute for Medical Research, Cambridge University, Cambridge, United Kingdom
    Blood 113:5783-92. 2009
    ..Finally, Sfpi1/Pu.1, Fli1, Gata2, Tal1/Scl, and Lmo2 were shown to bind to and transactivate Lmo2 hematopoietic enhancers, thus identifying key upstream regulators and positioning Lmo2 within hematopoietic regulatory networks...
  32. pmc Gene set control analysis predicts hematopoietic control mechanisms from genome-wide transcription factor binding data
    Anagha Joshi
    Department of Hematology, Cambridge Institute for Medical Research and Wellcome Trust and MRC Cambridge Stem Cell Institute, Cambridge University, Hills Road, Cambridge, UK Electronic address
    Exp Hematol 41:354-366.e14. 2013
    ..To facilitate access to the wider research community, we have implemented GSCA as a freely accessible web tool (http://bioinformatics.cscr.cam.ac.uk/GSCA/GSCA.html)...
  33. pmc Transcriptional link between blood and bone: the stem cell leukemia gene and its +19 stem cell enhancer are active in bone cells
    John E Pimanda
    Department of Haematology, Cambridge Institute for Medical Research, University of Cambridge, Cambridge CB2 2XY, United Kingdom
    Mol Cell Biol 26:2615-25. 2006
    ....
  34. ncbi The role of the stem cell leukemia (SCL) gene in hematopoietic and endothelial lineage specification
    Adrian J C Bloor
    Cambridge University Department of Haematology, Cambridge Institute for Medical Research, Hills Road, Cambridge, CB2 2XY, UK
    J Hematother Stem Cell Res 11:195-206. 2002
    ....
  35. pmc A novel mode of enhancer evolution: the Tal1 stem cell enhancer recruited a MIR element to specifically boost its activity
    Aileen M Smith
    University of Cambridge Department of Haematology, Cambridge Institute for Medical Research, Cambridge CB2 2XY, United Kingdom
    Genome Res 18:1422-32. 2008
    ..Our data demonstrate a previously unrecognized mechanism whereby enhancer activity is modulated by a transposon exerting a "booster" function which would go undetected by conventional transgenic approaches...
  36. pmc JAK2 phosphorylates histone H3Y41 and excludes HP1alpha from chromatin
    Mark A Dawson
    Cambridge Institute for Medical Research and Department of Haematology, University of Cambridge, Hills Road, Cambridge CB2 0XY, UK
    Nature 461:819-22. 2009
    ..Tauhese results identify a previously unrecognized nuclear role for JAK2 in the phosphorylation of H3Y41 and reveal a direct mechanistic link between two genes, jak2 and lmo2, involved in normal haematopoiesis and leukaemia...
  37. doi Deciphering transcriptional control mechanisms in hematopoiesis:the impact of high-throughput sequencing technologies
    Nicola K Wilson
    Department of Haematology, University of Cambridge, Cambridge Institute for Medical Research, UK
    Exp Hematol 39:961-8. 2011
    ..By focusing on successful insights from multiple different approaches, we hope to provide stimulating reading for anyone wanting to utilize ChIP-Seq technology within their particular research field...
  38. pmc cis-Regulatory remodeling of the SCL locus during vertebrate evolution
    Berthold Gottgens
    Department of Haematology, Cambridge Institute for Medical Research, University of Cambridge, Cambridge, United Kingdom
    Mol Cell Biol 30:5741-51. 2010
    ..Our data demonstrate remarkable cis-regulatory remodelling across the SCL locus and indicate that stable patterns of expression can mask extensive regulatory change. These insights illuminate our understanding of vertebrate evolution...
  39. pmc TFBScluster web server for the identification of mammalian composite regulatory elements
    Ian J Donaldson
    Department of Haematology, Cambridge Institute for Medical Research, University of Cambridge, Hills Road, Cambridge, CB2 2XY, UK
    Nucleic Acids Res 34:W524-8. 2006
    ..TFBScluster is publicly available at http://hscl.cimr.cam.ac.uk/TFBScluster_genome_portal.html...
  40. pmc Impaired in vitro erythropoiesis following deletion of the Scl (Tal1) +40 enhancer is largely compensated for in vivo despite a significant reduction in expression
    Rita Ferreira
    Department of Haematology, Cambridge Institute for Medical Research and Wellcome Trust and MRC Stem Cell Institute, University of Cambridge, Cambridge, United Kingdom
    Mol Cell Biol 33:1254-66. 2013
    ..Moreover, deletion of the +40 enhancer causes a novel erythroid phenotype, which can be rescued by ectopic expression of Scl but not Map17...
  41. pmc Deletion of the Scl +19 enhancer increases the blood stem cell compartment without affecting the formation of mature blood lineages
    Dominik Spensberger
    Department of Haematology, Cambridge Institute for Medical Research, University of Cambridge, Cambridge, UK
    Exp Hematol 40:588-598.e1. 2012
    ....
  42. pmc LIF-independent JAK signalling to chromatin in embryonic stem cells uncovered from an adult stem cell disease
    Dean S Griffiths
    Department of Haematology and Cambridge Institute for Medical Research, University of Cambridge, Cambridge CB2 0XY, UK
    Nat Cell Biol 13:13-21. 2011
    ..Taken together, these results uncover a previously unrecognized role for direct signalling to chromatin by JAK2 as an important mediator of ES cell self-renewal...
  43. pmc Genome-wide analysis of simultaneous GATA1/2, RUNX1, FLI1, and SCL binding in megakaryocytes identifies hematopoietic regulators
    Marloes R Tijssen
    Department of Haematology, Cambridge Institute for Medical Research, University of Cambridge, Cambridge CB2 0XY, UK
    Dev Cell 20:597-609. 2011
    ..Multifactor ChIP-Seq analysis in primary human cells coupled with a high-throughput in vivo perturbation screen therefore offers a powerful strategy to identify essential regulators of complex mammalian differentiation processes...
  44. ncbi Rescue of the lethal scl(-/-) phenotype by the human SCL locus
    Angus M Sinclair
    University of Cambridge, Department of Haematology, Cambridge Institute for Medical Research, Hills Road, Cambridge, CB2 2XY, United Kingdom
    Blood 99:3931-8. 2002
    ..In addition, our results demonstrate that the human SCL YAC contains the chromosomal domain necessary to direct expression to the erythroid lineage and to all other tissues in which SCL performs a nonredundant essential function...
  45. pmc Evolution of candidate transcriptional regulatory motifs since the human-chimpanzee divergence
    Ian J Donaldson
    Department of Haematology, Cambridge Institute for Medical Research, University of Cambridge, Cambridge, CB2 2XY, UK
    Genome Biol 7:R52. 2006
    ..Alteration in transcriptional regulatory mechanisms represents an important platform for evolutionary change, suggesting that a significant proportion of functional human-chimpanzee sequence differences may affect regulatory elements...
  46. pmc Three distinct patterns of histone H3Y41 phosphorylation mark active genes
    Mark A Dawson
    Gurdon Institute and Department of Pathology, Cambridge, UK
    Cell Rep 2:470-7. 2012
    ..Together, these data extend our understanding of this conserved and essential signaling pathway and provide insight into the mechanisms by which extracellular stimuli may lead to the coordinated regulation of transcription...
  47. pmc A GWAS sequence variant for platelet volume marks an alternative DNM3 promoter in megakaryocytes near a MEIS1 binding site
    Sylvia T Nürnberg
    Department of Haematology, University of Cambridge and National Health Service Blood and Transplant, Cambridge, United Kingdom
    Blood 120:4859-68. 2012
    ..The importance of dynamin activity to the latter stages of thrombopoiesis was confirmed by the observation that the inhibitor Dynasore reduced murine proplatelet for-mation in vitro...
  48. pmc Genome-wide analysis of transcriptional reprogramming in mouse models of acute myeloid leukaemia
    Nicolas Bonadies
    Department of Haematology, Cambridge Institute for Medical Research, Cambridge University, Cambridge, United Kingdom
    PLoS ONE 6:e16330. 2011
    ....
  49. ncbi Endoglin expression in the endothelium is regulated by Fli-1, Erg, and Elf-1 acting on the promoter and a -8-kb enhancer
    John E Pimanda
    Department of Hematology, Cambridge Institute of Medical Research, University of Cambridge, Cambridge CB2 2XY, UK
    Blood 107:4737-45. 2006
    ..This study therefore establishes the transcriptional framework within which endoglin functions during angiogenesis...
  50. doi Maximum parsimony analysis of gene expression profiles permits the reconstruction of developmental cell lineage trees
    Anagha Joshi
    Department of Haematology, Cambridge Institute for Medical Research, University of Cambridge, Hills Road, Cambridge, CB2 0XY, UK
    Dev Biol 353:440-7. 2011
    ....
  51. pmc A HaemAtlas: characterizing gene expression in differentiated human blood cells
    Nicholas A Watkins
    Department of Haematology, University of Cambridge, National Health Service Blood and Transplant, Cambridge, United Kingdom
    Blood 113:e1-9. 2009
    ..The data, which are freely accessible, will be invaluable for future studies on hematopoiesis and the role of specific genes and will also aid the understanding of the recent genome-wide association studies...
  52. pmc CoMoDis: composite motif discovery in mammalian genomes
    Ian J Donaldson
    Department of Haematology, Cambridge Institute for Medical Research, University of Cambridge, Hills Road, Cambridge CB2 2XY, UK
    Nucleic Acids Res 35:e1. 2007
    ..CoMoDis is available at http://hscl.cimr.cam.ac.uk/CoMoDis_portal.html...
  53. doi ID1 promotes expansion and survival of primary erythroid cells and is a target of JAK2V617F-STAT5 signaling
    Andrew D Wood
    Department of Hematology, Cambridge Institute for Medical Research, Addenbrooke s Hospital, Cambridge, United Kingdom
    Blood 114:1820-30. 2009
    ..Furthermore, our findings contribute to an increasing body of evidence implicating ID proteins in a wider range of cellular functions than initially appreciated...
  54. doi From genes to cells to tissues--modelling the haematopoietic system
    Samuel D Foster
    Haematopoietic Stem Cell Laboratory, Cambridge Institute for Medical Research, Wellcome Trust MRC Building, Hills Rd, Cambridge, CB2 0XY
    Mol Biosyst 5:1413-20. 2009
    ..Also discussed will be examples of how stochastic modelling may be applied to multi cell systems such as those seen in normal and malignant haematopoiesis...
  55. doi A compendium of genome-wide hematopoietic transcription factor maps supports the identification of gene regulatory control mechanisms
    Rebecca Hannah
    University of Cambridge, Department of Haematology, Cambridge Institute for Medical Research, Hills Road, Cambridge, UK
    Exp Hematol 39:531-41. 2011
    ..However, transcription factors operate in a combinatorial fashion suggesting that integrated analysis of genome-wide maps for multiple transcription factors will be essential to fully exploit these new genome-scale data sets...
  56. doi Ontogeny of haematopoiesis: recent advances and open questions
    Katrin Ottersbach
    Department of Haematology, Cambridge Institute for Medical Research, University of Cambridge, Cambridge, UK
    Br J Haematol 148:343-55. 2010
    ..In light of these rapid recent advances, research into the embryonic origins of the haematopoietic system should remain one of the most vibrant disciplines within the wider field of haematology for the foreseeable future...
  57. pmc The SCL gene specifies haemangioblast development from early mesoderm
    M Gering
    University of Cambridge, Department of Haematology, MRC Centre, Hills Road, Cambridge CB2 2QH, UK
    EMBO J 17:4029-45. 1998
    ..Our data also underline the striking similarities between the role of SCL in haematopoiesis/vasculogenesis and the function of other bHLH proteins in muscle and neural development...
  58. pmc Signaling from the sympathetic nervous system regulates hematopoietic stem cell emergence during embryogenesis
    Simon R Fitch
    Department of Haematology, Cambridge Institute for Medical Research, Wellcome Trust Medical Research Council Stem Cell Institute, University of Cambridge, Cambridge CB2 0XY, UK
    Cell Stem Cell 11:554-66. 2012
    ....
  59. doi Molecular basis of histone H3K36me3 recognition by the PWWP domain of Brpf1
    Alessandro Vezzoli
    MRC Centre for Protein Engineering, Cambridge, UK
    Nat Struct Mol Biol 17:617-9. 2010
    ..We have identified the PWWP domain of bromo and plant homeodomain (PHD) finger-containing protein 1 (BRPF1) as a H3K36me3 binding module and have determined the structure of this domain in complex with an H3K36me3-derived peptide...
  60. pmc BloodExpress: a database of gene expression in mouse haematopoiesis
    Diego Miranda-Saavedra
    Department of Haematology, Cambridge Institute for Medical Research, University of Cambridge, Hills Road, Cambridge CB2 0XY, UK
    Nucleic Acids Res 37:D873-9. 2009
    ..BloodExpress thus constitutes a platform for the discovery of novel gene functions across the haematopoietic tree. BloodExpress is freely accessible at http://hscl.cimr.cam.ac.uk/bloodexpress/...
  61. doi Transcriptional regulatory networks in haematopoiesis
    Diego Miranda-Saavedra
    Department of Haematology, Cambridge Institute for Medical Research, University of Cambridge, Cambridge, UK
    Curr Opin Genet Dev 18:530-5. 2008
    ..In this review, we outline how the integration of experimental and computational approaches as applied to haematopoiesis has resulted in some of the most advanced models of transcriptional regulatory networks in mammals...
  62. ncbi An SCL 3' enhancer targets developing endothelium together with embryonic and adult haematopoietic progenitors
    M Sanchez
    University of Cambridge, Department of Haematology, MRC Centre, Hills Road, Cambridge CB2 2QH, UK
    Development 126:3891-904. 1999
    ..This enhancer provides a potent tool for the manipulation of haematopoiesis and vasculogenesis in vivo...
  63. ncbi Distinct 5' SCL enhancers direct transcription to developing brain, spinal cord, and endothelium: neural expression is mediated by GATA factor binding sites
    A M Sinclair
    Department of Haematology, University of Cambridge, MRC Centre, Hills Road, Cambridge, CB2 2QH, United Kingdom
    Dev Biol 209:128-42. 1999
    ..These data suggest that SCL performs critical functions in neural development. The regulatory elements identified here provide important tools for analyzing these functions...
  64. ncbi Analysis of vertebrate SCL loci identifies conserved enhancers
    B Gottgens
    University of Cambridge, Department of Haematology, MRC Centre, Hills Road, Cambridge CB2 2QH, UK
    Nat Biotechnol 18:181-6. 2000
    ..This combination of long-range comparative sequence analysis with a high-throughput transgenic bioassay provides a powerful strategy for identifying and characterizing developmentally important enhancers...
  65. pmc Real-time PCR mapping of DNaseI-hypersensitive sites using a novel ligation-mediated amplification technique
    George A Follows
    Department of Haematology, Cambridge Institute for Medical Research, Addenbrooke s Hospital, University of Cambridge, Cambridge CB2 2XY, UK
    Nucleic Acids Res 35:e56. 2007
    ..Presented examples include comparative DHS mapping of known TAL1/SCL regulatory elements between human embryonic stem cells and K562 cells...
  66. pmc Identifying gene regulatory elements by genomic microarray mapping of DNaseI hypersensitive sites
    George A Follows
    Department of Haematology, Cambridge Institute for Medical Research, University of Cambridge, Cambridge, CB2 2XY, United Kingdom
    Genome Res 16:1310-9. 2006
    ..ADHM provides a powerful approach to identifying DNaseI hypersensitive sites across large genomic regions...
  67. doi Establishing the stem cell state: insights from regulatory network analysis of blood stem cell development
    Judith Schütte
    Department of Haematology, Cambridge Institute for Medical Research, University of Cambridge, Cambridge, UK
    Wiley Interdiscip Rev Syst Biol Med 4:285-95. 2012
    ..Moreover, lessons learned from regulatory network analysis in the hematopoietic system are likely to inform research on less experimentally tractable tissues...
  68. ncbi Discordant regulation of SCL/TAL-1 mRNA and protein during erythroid differentiation
    A M Murrell
    University of Cambridge, Department of Haematology, MRC Centre, UK
    Oncogene 11:131-9. 1995
    ..Up-regulation of transcription therefore accounted for most of the increase in SCL mRNA levels during erythroid differentiation...
  69. pmc The SCL +40 enhancer targets the midbrain together with primitive and definitive hematopoiesis and is regulated by SCL and GATA proteins
    S Ogilvy
    Department of Haematology, Cambridge Institute for Medical Research, University of Cambridge, Hills Road, Cambridge CB2 0XY, United Kingdom
    Mol Cell Biol 27:7206-19. 2007
    ....
  70. ncbi Distinct mechanisms direct SCL/tal-1 expression in erythroid cells and CD34 positive primitive myeloid cells
    E O Bockamp
    University of Cambridge, Department of Haematology, Medical Research Council Centre, Hills Road, Cambridge CB2 2QH, United Kingdom
    J Biol Chem 272:8781-90. 1997
    ..Our data therefore indicate that additional regulatory elements were necessary for both SCL promoters to overcome chromatin-mediated repression...
  71. pmc Long-range DNA looping and gene expression analyses identify DEXI as an autoimmune disease candidate gene
    Lucy J Davison
    Juvenile Diabetes Research Foundation Wellcome Trust Diabetes and Inflammation Laboratory, Department of Medical Genetics, NIHR Biomedical Research Centre, University of Cambridge, Cambridge, UK
    Hum Mol Genet 21:322-33. 2012
    ....
  72. ncbi Lineage-restricted regulation of the murine SCL/TAL-1 promoter
    E O Bockamp
    University of Cambridge, Department of Haematology, MRC Centre, UK
    Blood 86:1502-14. 1995
    ..These results suggest that inactivity of SCL promoter 1a in T cells reflected the absence of GATA-1 rather than the presence of trans-dominant negative regulators...
  73. doi A previously unrecognized promoter of LMO2 forms part of a transcriptional regulatory circuit mediating LMO2 expression in a subset of T-acute lymphoblastic leukaemia patients
    S H Oram
    Department of Haematology, Cambridge Institute for Medical Research, University of Cambridge, Cambridge, UK
    Oncogene 29:5796-808. 2010
    ..Our data therefore suggest that a self-sustaining triad of LMO2/ERG/FLI1 stabilizes the expression of important mediators of the leukaemic phenotype such as HHEX/PRH...
  74. pmc A tripartite transcription factor network regulates primordial germ cell specification in mice
    Erna Magnusdottir
    1 Wellcome Trust Cancer Research UK Gurdon Institute, University of Cambridge, Tennis Court Road, Cambridge CB2 1QN, UK 2 Department of Physiology, Development and Neuroscience, University of Cambridge, Downing Street, Cambridge CB2 3DY, UK 3
    Nat Cell Biol 15:905-15. 2013
    ..We also demonstrate that, in principle, BLIMP1, AP2γ and PRDM14 are sufficient for PGC specification, and the unprecedented resetting of the epigenome towards a basal state. ..