Research Topics
Genomes and Genes
| Berthold GottgensSummaryAffiliation: University of Cambridge Country: UK Publications
| Collaborators
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Detail Information
Publications
A new RNASeq-based reference transcriptome for sugar beet and its application in transcriptome-scale analysis of vernalization and gibberellin responsesEffie S Mutasa-Göttgens
Rothamsted Research Broom s Barn, Department of Applied Crop Science, Higham, Bury St Edmunds, Suffolk IP26 6NP, UK
BMC Genomics 13:99. 2012..In this study, we demonstrate the application of an mRNA-Seq based strategy for this purpose...
Evolution of candidate transcriptional regulatory motifs since the human-chimpanzee divergenceIan J Donaldson
Department of Haematology, Cambridge Institute for Medical Research, University of Cambridge, Cambridge, CB2 2XY, UK
Genome Biol 7:R52. 2006..Moreover, genes associated with mutated candidate transcription-factor binding sites highlight potential pathways underlying human-chimpanzee divergence...- Fast and sensitive multiple alignment of large genomic sequencesMichael Brudno
Department of Computer Science, Stanford University, Stanford, CA 94305, USA
BMC Bioinformatics 4:66. 2003..In a first step, a fast search program identifies a chain of strong local sequence similarities. In a second step, regions between these anchor points are aligned using a slower but more accurate method... 
Transcriptional regulation of haematopoiesisB Gottgens
Department of Haematology, Cambridge University, Cambridge Institute for Medical Research, Hills Road, Cambridge CB2 2XY, UK
Vox Sang 87:15-9. 2004- Fli1, Elf1, and Ets1 regulate the proximal promoter of the LMO2 gene in endothelial cellsJosette Renee Landry
Department of Haematology, Cambridge Institute for Medical Research, Cambridge University, Hills Rd, Cambridge, CB2 2XY, United Kingdom
Blood 106:2680-7. 2005..Together, these results suggest that the conserved proximal promoter is central to LMO2 transcription in hematopoietic and endothelial cells, where it is regulated by Ets factors... - The scl +18/19 stem cell enhancer is not required for hematopoiesis: identification of a 5' bifunctional hematopoietic-endothelial enhancer bound by Fli-1 and Elf-1Berthold Gottgens
Department of Hematology, Cambridge Institute for Medical Research, University of Cambridge, United Kingdom
Mol Cell Biol 24:1870-83. 2004.... - Establishing the transcriptional programme for blood: the SCL stem cell enhancer is regulated by a multiprotein complex containing Ets and GATA factorsBerthold Gottgens
University of Cambridge Department of Haematology, Cambridge Institute for Medical Research, Hills Road, Cambridge CB2 2XY, UK
EMBO J 21:3039-50. 2002..Our results suggest that these transcription factors are key components of an enhanceosome responsible for activating SCL transcription and establishing the transcriptional programme required for HSC formation... - Transcriptional regulation of the stem cell leukemia gene (SCL)--comparative analysis of five vertebrate SCL lociBerthold Gottgens
Cambridge Institute for Medical Research, Cambridge University, Cambridge, CB2 2XY, United Kingdom
Genome Res 12:749-59. 2002..Analysis of the SCL promoter/enhancer revealed five motifs, which were conserved from zebrafish to mammals, and each of which is essential for the appropriate pattern or level of SCL transcription... - Long-range comparison of human and mouse SCL loci: localized regions of sensitivity to restriction endonucleases correspond precisely with peaks of conserved noncoding sequencesB Gottgens
The Wellcome Trust Centre for Molecular Mechanisms in Disease, Cambridge Institute for Medical Research, Addenbrooke s Hospital Site, Cambridge CB2 2XY, UK
Genome Res 11:87-97. 2001.... - Transcriptional regulation of haematopoietic transcription factorsNicola K Wilson
University of Cambridge Department of Haematology, Cambridge Institute for Medical Research, Hills Road, Cambridge, CB2 0XY, UK
Stem Cell Res Ther 2:6. 2011..We also explore how changes in technology will impact on this area of research in the future... - Tdr2, a new zebrafish transposon of the Tc1 familyB Gottgens
Department of Haematology, Cambridge University, MRC Centre, Hills Road, Cambridge, UK
Gene 239:373-9. 1999..Tdr2 transposons may facilitate the development of novel transposon-based tools for the genetic analysis of zebrafish... - Integration of Elf-4 into stem/progenitor and erythroid regulatory networks through locus-wide chromatin studies coupled with in vivo functional validationAileen M Smith
University of Cambridge Department of Haematology, Cambridge Institute for Medical Research, Cambridge, United Kingdom
Mol Cell Biol 32:763-73. 2012.... - Transcriptional regulation of Elf-1: locus-wide analysis reveals four distinct promoters, a tissue-specific enhancer, control by PU.1 and the importance of Elf-1 downregulation for erythroid maturationFernando J Calero-Nieto
Department of Haematology, Cambridge Institute for Medical Research, Cambridge University, Hills Road, Cambridge CB2 0XY, UK
Nucleic Acids Res 38:6363-74. 2010..1 controls myeloid-erythroid differentiation. Our findings therefore not only represent the first report of Elf-1 regulation but also enhance our understanding of the wider regulatory networks that control haematopoiesis... - The SCL transcriptional network and BMP signaling pathway interact to regulate RUNX1 activityJohn E Pimanda
Cambridge Institute for Medical Research, University of Cambridge, Cambridge CB2 2XY, United Kingdom
Proc Natl Acad Sci U S A 104:840-5. 2007..Taken together, our results integrate three key determinants of HSC development; the Scl transcriptional network, Runx1 activity, and the Bmp4/Smad signaling pathway... 
Runx genes are direct targets of Scl/Tal1 in the yolk sac and fetal liverJosette Renee Landry
Department of Haematology, Cambridge Institute for Medical Research, Cambridge University, Cambridge, United Kingdom
Blood 111:3005-14. 2008..Together, our data provide a key component of the transcriptional network of early hematopoiesis by identifying downstream targets of Scl that can explain key aspects of the early Scl(-/-) phenotype...- Endoglin expression in blood and endothelium is differentially regulated by modular assembly of the Ets/Gata hemangioblast codeJohn E Pimanda
Department of Haematology, Cambridge Institute for Medical Research, University of Cambridge, Cambridge, United Kingdom
Blood 112:4512-22. 2008..This modular assembly of regulators gives blood and endothelial cells the regulatory freedom to independently fine-tune gene expression and emphasizes the role of regulatory divergence in driving functional divergence... - The transcriptional program controlled by the stem cell leukemia gene Scl/Tal1 during early embryonic hematopoietic developmentNicola K Wilson
University of Cambridge, Department of Haematology, Cambridge Institute for Medical Research, Cambridge, United Kingdom
Blood 113:5456-65. 2009.... - Mapping and functional characterisation of a CTCF-dependent insulator element at the 3' border of the murine Scl transcriptional domainGeorge A Follows
Department of Haematology, Cambridge Institute for Medical Research, University of Cambridge, Cambridge, United Kingdom
PLoS ONE 7:e31484. 2012.... - The transcriptional coactivator Cbp regulates self-renewal and differentiation in adult hematopoietic stem cellsWai In Chan
Department of Haematology, University of Cambridge, Cambridge Institute for Medical Research, Cambridge CB2 0XY, United Kingdom
Mol Cell Biol 31:5046-60. 2011..Our data demonstrate that Cbp plays a role in adult HSC homeostasis by maintaining the balance between different HSC fate decisions, and our findings identify a putative HSC-specific transcriptional network coordinated by Cbp... - The paralogous hematopoietic regulators Lyl1 and Scl are coregulated by Ets and GATA factors, but Lyl1 cannot rescue the early Scl-/- phenotypeWan Y I Chan
Department of Haematology, Cambridge Institute for Medical Research, University of Cambridge, United Kingdom
Blood 109:1908-16. 2007..Furthermore, coregulation of Scl and Lyl1 later during development may explain the mild phenotype of Scl-/- adult HSCs... - Gfi1 expression is controlled by five distinct regulatory regions spread over 100 kilobases, with Scl/Tal1, Gata2, PU.1, Erg, Meis1, and Runx1 acting as upstream regulators in early hematopoietic cellsNicola K Wilson
University of Cambridge, Department of Haematology, Cambridge Institute for Medical Research, Cambridge, United Kingdom
Mol Cell Biol 30:3853-63. 2010.... - Gata2, Fli1, and Scl form a recursively wired gene-regulatory circuit during early hematopoietic developmentJohn E Pimanda
Department of Haematology, Cambridge Institute for Medical Research, University of Cambridge, Cambridge CB2 0XY, United Kingdom
Proc Natl Acad Sci U S A 104:17692-7. 2007.... - Expression of the leukemia oncogene Lmo2 is controlled by an array of tissue-specific elements dispersed over 100 kb and bound by Tal1/Lmo2, Ets, and Gata factorsJosette Renee Landry
Department of Haematology, Cambridge Institute for Medical Research, Cambridge University, Cambridge, United Kingdom
Blood 113:5783-92. 2009..Finally, Sfpi1/Pu.1, Fli1, Gata2, Tal1/Scl, and Lmo2 were shown to bind to and transactivate Lmo2 hematopoietic enhancers, thus identifying key upstream regulators and positioning Lmo2 within hematopoietic regulatory networks... - A novel mode of enhancer evolution: the Tal1 stem cell enhancer recruited a MIR element to specifically boost its activityAileen M Smith
University of Cambridge Department of Haematology, Cambridge Institute for Medical Research, Cambridge CB2 2XY, United Kingdom
Genome Res 18:1422-32. 2008..Our data demonstrate a previously unrecognized mechanism whereby enhancer activity is modulated by a transposon exerting a "booster" function which would go undetected by conventional transgenic approaches... - Combinatorial transcriptional control in blood stem/progenitor cells: genome-wide analysis of ten major transcriptional regulatorsNicola K Wilson
University of Cambridge Department of Haematology, Cambridge Institute for Medical Research, Hills Road, Cambridge, CB2 0XY, UK
Cell Stem Cell 7:532-44. 2010..Taken together, this study demonstrates the power of genome-wide analysis in generating novel functional insights into the transcriptional control of stem and progenitor cells... - Transcriptional link between blood and bone: the stem cell leukemia gene and its +19 stem cell enhancer are active in bone cellsJohn E Pimanda
Department of Haematology, Cambridge Institute for Medical Research, University of Cambridge, Cambridge CB2 2XY, United Kingdom
Mol Cell Biol 26:2615-25. 2006.... - Deciphering transcriptional control mechanisms in hematopoiesis:the impact of high-throughput sequencing technologiesNicola K Wilson
Department of Haematology, University of Cambridge, Cambridge Institute for Medical Research, UK
Exp Hematol 39:961-8. 2011..By focusing on successful insights from multiple different approaches, we hope to provide stimulating reading for anyone wanting to utilize ChIP-Seq technology within their particular research field... - cis-Regulatory remodeling of the SCL locus during vertebrate evolutionBerthold Gottgens
Department of Haematology, Cambridge Institute for Medical Research, University of Cambridge, Cambridge, United Kingdom
Mol Cell Biol 30:5741-51. 2010..Our data demonstrate remarkable cis-regulatory remodelling across the SCL locus and indicate that stable patterns of expression can mask extensive regulatory change. These insights illuminate our understanding of vertebrate evolution... - JAK2 phosphorylates histone H3Y41 and excludes HP1alpha from chromatinMark A Dawson
Cambridge Institute for Medical Research and Department of Haematology, University of Cambridge, Hills Road, Cambridge CB2 0XY, UK
Nature 461:819-22. 2009..Tauhese results identify a previously unrecognized nuclear role for JAK2 in the phosphorylation of H3Y41 and reveal a direct mechanistic link between two genes, jak2 and lmo2, involved in normal haematopoiesis and leukaemia... - Deletion of the Scl +19 enhancer increases the blood stem cell compartment without affecting the formation of mature blood lineagesDominik Spensberger
Department of Haematology, Cambridge Institute for Medical Research, University of Cambridge, Cambridge, UK
Exp Hematol 40:588-598.e1. 2012.... - LIF-independent JAK signalling to chromatin in embryonic stem cells uncovered from an adult stem cell diseaseDean S Griffiths
Department of Haematology and Cambridge Institute for Medical Research, University of Cambridge, Cambridge CB2 0XY, UK
Nat Cell Biol 13:13-21. 2011..Taken together, these results uncover a previously unrecognized role for direct signalling to chromatin by JAK2 as an important mediator of ES cell self-renewal... - Genome-wide analysis of simultaneous GATA1/2, RUNX1, FLI1, and SCL binding in megakaryocytes identifies hematopoietic regulatorsMarloes R Tijssen
Department of Haematology, Cambridge Institute for Medical Research, University of Cambridge, Cambridge CB2 0XY, UK
Dev Cell 20:597-609. 2011..Multifactor ChIP-Seq analysis in primary human cells coupled with a high-throughput in vivo perturbation screen therefore offers a powerful strategy to identify essential regulators of complex mammalian differentiation processes... - Rescue of the lethal scl(-/-) phenotype by the human SCL locusAngus M Sinclair
University of Cambridge, Department of Haematology, Cambridge Institute for Medical Research, Hills Road, Cambridge, CB2 2XY, United Kingdom
Blood 99:3931-8. 2002..In addition, our results demonstrate that the human SCL YAC contains the chromosomal domain necessary to direct expression to the erythroid lineage and to all other tissues in which SCL performs a nonredundant essential function... - Endoglin expression in the endothelium is regulated by Fli-1, Erg, and Elf-1 acting on the promoter and a -8-kb enhancerJohn E Pimanda
Department of Hematology, Cambridge Institute of Medical Research, University of Cambridge, Cambridge CB2 2XY, UK
Blood 107:4737-45. 2006..This study therefore establishes the transcriptional framework within which endoglin functions during angiogenesis... - Genome-wide analysis of transcriptional reprogramming in mouse models of acute myeloid leukaemiaNicolas Bonadies
Department of Haematology, Cambridge Institute for Medical Research, Cambridge University, Cambridge, United Kingdom
PLoS ONE 6:e16330. 2011.... - Maximum parsimony analysis of gene expression profiles permits the reconstruction of developmental cell lineage treesAnagha Joshi
Department of Haematology, Cambridge Institute for Medical Research, University of Cambridge, Hills Road, Cambridge, CB2 0XY, UK
Dev Biol 353:440-7. 2011.... - A HaemAtlas: characterizing gene expression in differentiated human blood cellsNicholas A Watkins
Department of Haematology, University of Cambridge, National Health Service Blood and Transplant, Cambridge, United Kingdom
Blood 113:e1-9. 2009..The data, which are freely accessible, will be invaluable for future studies on hematopoiesis and the role of specific genes and will also aid the understanding of the recent genome-wide association studies... - Three distinct patterns of histone H3Y41 phosphorylation mark active genesMark A Dawson
Gurdon Institute and Department of Pathology, Cambridge, UK
Cell Rep 2:470-7. 2012..Together, these data extend our understanding of this conserved and essential signaling pathway and provide insight into the mechanisms by which extracellular stimuli may lead to the coordinated regulation of transcription... - From genes to cells to tissues--modelling the haematopoietic systemSamuel D Foster
Haematopoietic Stem Cell Laboratory, Cambridge Institute for Medical Research, Wellcome Trust MRC Building, Hills Rd, Cambridge, CB2 0XY
Mol Biosyst 5:1413-20. 2009..Also discussed will be examples of how stochastic modelling may be applied to multi cell systems such as those seen in normal and malignant haematopoiesis... - Ontogeny of haematopoiesis: recent advances and open questionsKatrin Ottersbach
Department of Haematology, Cambridge Institute for Medical Research, University of Cambridge, Cambridge, UK
Br J Haematol 148:343-55. 2010..In light of these rapid recent advances, research into the embryonic origins of the haematopoietic system should remain one of the most vibrant disciplines within the wider field of haematology for the foreseeable future... - ID1 promotes expansion and survival of primary erythroid cells and is a target of JAK2V617F-STAT5 signalingAndrew D Wood
Department of Hematology, Cambridge Institute for Medical Research, Addenbrooke s Hospital, Cambridge, United Kingdom
Blood 114:1820-30. 2009..Furthermore, our findings contribute to an increasing body of evidence implicating ID proteins in a wider range of cellular functions than initially appreciated... - A compendium of genome-wide hematopoietic transcription factor maps supports the identification of gene regulatory control mechanismsRebecca Hannah
University of Cambridge, Department of Haematology, Cambridge Institute for Medical Research, Hills Road, Cambridge, UK
Exp Hematol 39:531-41. 2011..However, transcription factors operate in a combinatorial fashion suggesting that integrated analysis of genome-wide maps for multiple transcription factors will be essential to fully exploit these new genome-scale data sets... - CoMoDis: composite motif discovery in mammalian genomesIan J Donaldson
Department of Haematology, Cambridge Institute for Medical Research, University of Cambridge, Hills Road, Cambridge CB2 2XY, UK
Nucleic Acids Res 35:e1. 2007..CoMoDis is available at http://hscl.cimr.cam.ac.uk/CoMoDis_portal.html... - The SCL gene specifies haemangioblast development from early mesodermM Gering
University of Cambridge, Department of Haematology, MRC Centre, Hills Road, Cambridge CB2 2QH, UK
EMBO J 17:4029-45. 1998..Our data also underline the striking similarities between the role of SCL in haematopoiesis/vasculogenesis and the function of other bHLH proteins in muscle and neural development... - Molecular basis of histone H3K36me3 recognition by the PWWP domain of Brpf1Alessandro Vezzoli
MRC Centre for Protein Engineering, Cambridge, UK
Nat Struct Mol Biol 17:617-9. 2010..We have identified the PWWP domain of bromo and plant homeodomain (PHD) finger-containing protein 1 (BRPF1) as a H3K36me3 binding module and have determined the structure of this domain in complex with an H3K36me3-derived peptide... - Real-time PCR mapping of DNaseI-hypersensitive sites using a novel ligation-mediated amplification techniqueGeorge A Follows
Department of Haematology, Cambridge Institute for Medical Research, Addenbrooke s Hospital, University of Cambridge, Cambridge CB2 2XY, UK
Nucleic Acids Res 35:e56. 2007..Presented examples include comparative DHS mapping of known TAL1/SCL regulatory elements between human embryonic stem cells and K562 cells... - Identifying gene regulatory elements by genomic microarray mapping of DNaseI hypersensitive sitesGeorge A Follows
Department of Haematology, Cambridge Institute for Medical Research, University of Cambridge, Cambridge, CB2 2XY, United Kingdom
Genome Res 16:1310-9. 2006..ADHM provides a powerful approach to identifying DNaseI hypersensitive sites across large genomic regions... - BloodExpress: a database of gene expression in mouse haematopoiesisDiego Miranda-Saavedra
Department of Haematology, Cambridge Institute for Medical Research, University of Cambridge, Hills Road, Cambridge CB2 0XY, UK
Nucleic Acids Res 37:D873-9. 2009..BloodExpress thus constitutes a platform for the discovery of novel gene functions across the haematopoietic tree. BloodExpress is freely accessible at http://hscl.cimr.cam.ac.uk/bloodexpress/... - Transcriptional regulatory networks in haematopoiesisDiego Miranda-Saavedra
Department of Haematology, Cambridge Institute for Medical Research, University of Cambridge, Cambridge, UK
Curr Opin Genet Dev 18:530-5. 2008..In this review, we outline how the integration of experimental and computational approaches as applied to haematopoiesis has resulted in some of the most advanced models of transcriptional regulatory networks in mammals... - An SCL 3' enhancer targets developing endothelium together with embryonic and adult haematopoietic progenitorsM Sanchez
University of Cambridge, Department of Haematology, MRC Centre, Hills Road, Cambridge CB2 2QH, UK
Development 126:3891-904. 1999..This enhancer provides a potent tool for the manipulation of haematopoiesis and vasculogenesis in vivo... - Distinct 5' SCL enhancers direct transcription to developing brain, spinal cord, and endothelium: neural expression is mediated by GATA factor binding sitesA M Sinclair
Department of Haematology, University of Cambridge, MRC Centre, Hills Road, Cambridge, CB2 2QH, United Kingdom
Dev Biol 209:128-42. 1999..These data suggest that SCL performs critical functions in neural development. The regulatory elements identified here provide important tools for analyzing these functions... - Analysis of vertebrate SCL loci identifies conserved enhancersB Gottgens
University of Cambridge, Department of Haematology, MRC Centre, Hills Road, Cambridge CB2 2QH, UK
Nat Biotechnol 18:181-6. 2000..This combination of long-range comparative sequence analysis with a high-throughput transgenic bioassay provides a powerful strategy for identifying and characterizing developmentally important enhancers... - Establishing the stem cell state: insights from regulatory network analysis of blood stem cell developmentJudith Schütte
Department of Haematology, Cambridge Institute for Medical Research, University of Cambridge, Cambridge, UK
Wiley Interdiscip Rev Syst Biol Med 4:285-95. 2012..Moreover, lessons learned from regulatory network analysis in the hematopoietic system are likely to inform research on less experimentally tractable tissues... - Impaired In Vitro Erythropoiesis following Deletion of the Scl (Tal1) +40 Enhancer Is Largely Compensated for In Vivo despite a Significant Reduction in ExpressionRita Ferreira
Department of Haematology, Cambridge Institute for Medical Research and Wellcome Trust and MRC Stem Cell Institute, University of Cambridge, Cambridge, United Kingdom
Mol Cell Biol 33:1254-66. 2013..Moreover, deletion of the +40 enhancer causes a novel erythroid phenotype, which can be rescued by ectopic expression of Scl but not Map17... - Discordant regulation of SCL/TAL-1 mRNA and protein during erythroid differentiationA M Murrell
University of Cambridge, Department of Haematology, MRC Centre, UK
Oncogene 11:131-9. 1995..Up-regulation of transcription therefore accounted for most of the increase in SCL mRNA levels during erythroid differentiation... - The SCL +40 enhancer targets the midbrain together with primitive and definitive hematopoiesis and is regulated by SCL and GATA proteinsS Ogilvy
Department of Haematology, Cambridge Institute for Medical Research, University of Cambridge, Hills Road, Cambridge CB2 0XY, United Kingdom
Mol Cell Biol 27:7206-19. 2007.... - Distinct mechanisms direct SCL/tal-1 expression in erythroid cells and CD34 positive primitive myeloid cellsE O Bockamp
University of Cambridge, Department of Haematology, Medical Research Council Centre, Hills Road, Cambridge CB2 2QH, United Kingdom
J Biol Chem 272:8781-90. 1997..Our data therefore indicate that additional regulatory elements were necessary for both SCL promoters to overcome chromatin-mediated repression... - Signaling from the sympathetic nervous system regulates hematopoietic stem cell emergence during embryogenesisSimon R Fitch
Department of Haematology, Cambridge Institute for Medical Research, Wellcome Trust Medical Research Council Stem Cell Institute, University of Cambridge, Cambridge CB2 0XY, UK
Cell Stem Cell 11:554-66. 2012.... - Long-range DNA looping and gene expression analyses identify DEXI as an autoimmune disease candidate geneLucy J Davison
Juvenile Diabetes Research Foundation Wellcome Trust Diabetes and Inflammation Laboratory, Department of Medical Genetics, NIHR Biomedical Research Centre, University of Cambridge, Cambridge, UK
Hum Mol Genet 21:322-33. 2012.... - Lineage-restricted regulation of the murine SCL/TAL-1 promoterE O Bockamp
University of Cambridge, Department of Haematology, MRC Centre, UK
Blood 86:1502-14. 1995..These results suggest that inactivity of SCL promoter 1a in T cells reflected the absence of GATA-1 rather than the presence of trans-dominant negative regulators... - Esrrb is a pivotal target of the Gsk3/Tcf3 axis regulating embryonic stem cell self-renewalGraziano Martello
Wellcome Trust Medical Research Council Stem Cell Institute, University of Cambridge, Cambridge UK
Cell Stem Cell 11:491-504. 2012..These findings highlight a key role for Esrrb in regulating the naive pluripotent state and illustrate compensation among the core pluripotency factors... - A previously unrecognized promoter of LMO2 forms part of a transcriptional regulatory circuit mediating LMO2 expression in a subset of T-acute lymphoblastic leukaemia patientsS H Oram
Department of Haematology, Cambridge Institute for Medical Research, University of Cambridge, Cambridge, UK
Oncogene 29:5796-808. 2010..Our data therefore suggest that a self-sustaining triad of LMO2/ERG/FLI1 stabilizes the expression of important mediators of the leukaemic phenotype such as HHEX/PRH... - A GWAS sequence variant for platelet volume marks an alternative DNM3 promoter in megakaryocytes near a MEIS1 binding siteSylvia T Nürnberg
Department of Haematology, University of Cambridge and National Health Service Blood and Transplant, Cambridge, United Kingdom
Blood 120:4859-68. 2012..The importance of dynamin activity to the latter stages of thrombopoiesis was confirmed by the observation that the inhibitor Dynasore reduced murine proplatelet for-mation in vitro...