Research Topics
| S H GillespieSummaryAffiliation: University College London Country: UK Publications
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Detail Information
Publications
Early bactericidal activity of a moxifloxacin and isoniazid combination in smear-positive pulmonary tuberculosisStephen H Gillespie
Centre for Medical Microbiology, Hampstead Campus, Royal Free and University College Medical School, Rowland Hill Street, London NW3 2PF, UK
J Antimicrob Chemother 56:1169-71. 2005..In vitro and animal studies have shown that moxifloxacin-containing combinations may improve the bactericidal efficacy of antituberculosis regimens...- Tuberculosis: evolution in millennia and minutesS H Gillespie
Centre for Medical Microbiology, Hampstead Campus, Rowland Hill Street, London NW3 2PF, U K
Biochem Soc Trans 35:1317-20. 2007..M. tuberculosis is a highly effective pathogen that has caused disease in human populations for millennia. We are now starting to understand some of the genetic mechanisms behind this phenomenon... 
Approaches to measure the fitness of Burkholderia cepacia complex isolatesC F Pope
Centre for Clinical Microbiology, University College London, Rowland Hill Street, London NW3 2QG, UK
J Med Microbiol 59:679-86. 2010..Unlike in Pseudomonas aeruginosa where hypermutability has been detected in strains isolated from CF patients, we were unable to demonstrate hypermutability in this panel of Burkholderia cenocepacia and Burkholderia multivorans isolates...
Mycobacterium tuberculosis DNA repair in response to subinhibitory concentrations of ciprofloxacinD M O'Sullivan
Centre for Medical Microbiology, Department of Infection, Royal Free Campus, University College London, Rowland Hill Street, Hampstead, London NW3 2PF, UK
J Antimicrob Chemother 62:1199-202. 2008..To investigate how the SOS response, an error-prone DNA repair pathway, is expressed following subinhibitory quinolone treatment of Mycobacterium tuberculosis...- Mycobacterium tuberculosis lineage: a naming of the partsT D McHugh
Department of Infection, Centre for Medical Microbiology, University College London, Royal Free Campus, Rowland Hill Street, Hampstead, London NW3 2PF, UK
Tuberculosis (Edinb) 85:127-36. 2005..Such a framework will permit associations between a lineage and clinical or bacterial phenomenon to be tested objectively. This definition will also enable new putative lineages to be objectively tested... - A multicentre comparison of a novel surrogate marker for determining the specific potency of anti-tuberculosis drugsRoly D Gosling
Department of Medical Microbiology, University College London, Royal Free Campus, Rowland Hill Street, London NW3 2PF, UK
J Antimicrob Chemother 52:473-6. 2003..From this, we conclude that the reiterative exponential decay model permits comparison between the data obtained in different centres and would allow the activity of a new drug to be compared with that of the currently available agents... - The effect of oxidative stress on the mutation rate of Mycobacterium tuberculosis with impaired catalase/peroxidase functionD M O'Sullivan
Centre for Medical Microbiology, Department of Infection, Hampstead Campus, University College London, Rowland Hill Street, Hampstead, London NW3 2PF, UK
J Antimicrob Chemother 62:709-12. 2008..To determine the effect of oxidative stress on isoniazid-resistant Mycobacterium tuberculosis deficient in catalase/peroxidase activity to varying degrees through mutation in katG... - Clinical application of a rapid lung-orientated immunoassay in individuals with possible tuberculosisR A M Breen
Department of Immunology, Royal Free and University College Medical School, London, UK
Thorax 63:67-71. 2008..Lung sampling enables combined microbiological and immunological testing and uses higher frequency antigen-specific responses than in blood... - The interpretation of nucleic acid amplification tests for tuberculosis: do rapid tests change treatment decisions?S J Conaty
Department of Primary Care and Population Science, UCL Centre for Infectious Disease Epidemiology, Royal Free and University College Medical School, Royal Free Campus, London NW3 2PF, UK
J Infect 50:187-92. 2005..To describe changes in treatment decisions after receipt of nucleic acid amplification (NAA) test for the diagnosis of M. tuberculosis... - Antibiotic resistance in the absence of selective pressureS H Gillespie
Royal Free and University College Medical School, Royal Free Campus, Rowland Hill Street, London NW3 2PF, UK
Int J Antimicrob Agents 17:171-6. 2001..Ultimately, we must look again to new drug discovery to improve our therapeutic armoury... - Prospective evaluation of BDProbeTec strand displacement amplification (SDA) system for diagnosis of tuberculosis in non-respiratory and respiratory samplesT D McHugh
Centre for Medical Microbiology, Department of Infection, Royal Free and University College Medical School, Pond Street, London NW3 2PF, UK
J Med Microbiol 53:1215-9. 2004..Specimen type did not predict likelihood of a sample being in the low-positive zone. Although the manufacturers do not describe the concept of a low-positive zone, we have found that it aids clinical diagnosis... - A novel method for evaluating the antimicrobial activity of tuberculosis treatment regimensS H Gillespie
Department of Medical Microbiology, University College London, London, United Kingdom
Int J Tuberc Lung Dis 7:684-9. 2003..To evaluate the clinical response to antituberculosis chemotherapy rapidly... - A comparison of the bactericidal activity of quinolone antibiotics in a Mycobacterium fortuitum modelS H Gillespie
Royal Free and University College Medical School, London
J Med Microbiol 50:565-70. 2001..These data indicate that moxifloxacin was the most bactericidal of the fluoroquinolones tested. Such methods provide a simple in-vitro measure that correlates with in-vivo models... - Evolutionary barriers to quinolone resistance in Streptococcus pneumoniaeStephen H Gillespie
Department of Medical Microbiology, Royal Free and University College Medical School, London, UK
Microb Drug Resist 8:79-84. 2002..16 (95% C.I. +/- 0.17), 0.99 (95% C.I. +/- 0.05), and 0.95 (95% C.I. +/- 0.05), respectively. These data suggest that mutation in the parC and gyrA genes may, on some occasions, not be associated with a physiological deficit... - Novel PCR-restriction fragment length polymorphism method for determining serotypes or serogroups of Streptococcus pneumoniae isolatesSarah L Batt
Centre for Medical Microbiology, University College London, Royal Free Campus, Rowland Hill St, London NW3 2PF, United Kingdom
J Clin Microbiol 43:2656-61. 2005..Once our database contains all 90 serotypes, this technique should be fully portable, cost-effective, and useful in any laboratory with sufficient molecular experience... - A reiterative method for calculating the early bactericidal activity of antituberculosis drugsStephen H Gillespie
Academic Department of Medical Microbiology, Royal Free and University College Medical School, London, United Kingdom
Am J Respir Crit Care Med 166:31-5. 2002.... - Microbes sans frontièresStephen H Gillespie
Department of Medical Microbiology, University College London, Royal Free Campus, NW3 2PF, London, UK
Lancet 359:93-5. 2002 - Multiple drug-resistant Mycobacterium tuberculosis: evidence for changing fitness following passage through human hostsStephen H Gillespie
Department of Medical Microbiology, Royal Free and University College Medical School, London NW3 2PF, UK
Microb Drug Resist 8:273-9. 2002..This study provides the first evidence that multiple drug resistant M. tuberculosis strains adapt to the environment of their human host... - Fluoroquinolone resistance in Streptococcus pneumoniae: evidence that gyrA mutations arise at a lower rate and that mutation in gyrA or parC predisposes to further mutationStephen H Gillespie
Department of Medical Microbiology, Royal Free and University College Medical School, London NW3 2PF, UK
Microb Drug Resist 9:17-24. 2003..3 x 10(-8) for parC Serine 79 Tyrosine and 7.2 x 10(-9) for gyrA Serine 81 Phenylalanine, 12 and 450 times higher, respectively, than for first-step rates, suggesting that mutation in either gene readies the genome for further mutation... - Evolution of drug resistance in Mycobacterium tuberculosis: clinical and molecular perspectiveStephen H Gillespie
Royal Free and University College Medical School, University College London, London NW3 2PF, United Kingdom
Antimicrob Agents Chemother 46:267-74. 2002 - New tricks from an old dog: streptococcal necrotising soft-tissue infectionsStephen H Gillespie
University College London, Royal Free Campus, London NW3 2PF, UK
Lancet 363:672-3. 2004 - Biomarkers of treatment response in clinical trials of novel antituberculosis agentsFelicity M R Perrin
Centre of Medical Microbiology, Royal Free and University College Medical School, London, UK
Lancet Infect Dis 7:481-90. 2007..Finally, we propose methods to assess candidate markers, and how these candidate markers could be implemented in future clinical trials... - Resuscitation-promoting factors are expressed in Mycobacterium tuberculosis-infected human tissueA P Davies
Centre for Medical Microbiology, Department of Infection, Royal Free and University College Medical School, London, UK
Tuberculosis (Edinb) 88:462-8. 2008..The work presented here demonstrates that Rpf expression can be detected in human tissues infected with M. tuberculosis and describes the pattern of this expression using immunocytochemistry with anti-Rpf antibodies... - Fluoroquinolone-resistant mutants of Burkholderia cepaciaC F Pope
Centre for Medical Microbiology, Royal Free and University College Medical School, Rowland Hill Street, London NW3 2PF, United Kingdom
Antimicrob Agents Chemother 52:1201-3. 2008..Growth rate, biofilm formation, and survival in water and during drying were not impaired in strains containing single gyrA mutations. Double mutants were impaired only in growth rate (0.85, relative to the susceptible parent)... - Think TB! Is the diagnosis of pulmonary tuberculosis delayed by the use of antibiotics?S E Craig
Department of Respiratory Medicine, Royal Free Hospital, and Department of Medical Statistics and Epidemiology, Royal Free and University College Medical School, London, UK
Int J Tuberc Lung Dis 13:208-13. 2009..Effective tuberculosis (TB) control requires prompt diagnosis of infectious cases through early suspicion of pulmonary TB in all subjects with suspected respiratory infection... - Analysis of rpoB and pncA mutations in the published literature: an insight into the role of oxidative stress in Mycobacterium tuberculosis evolution?Denise M O'Sullivan
Centre for Medical Microbiology, Department of Infection, Royal Free and University College Medical School, Royal Free Campus, University College London, Rowland Hill Street, London NW3 2PF, UK
J Antimicrob Chemother 55:674-9. 2005..Various mutations are thought to arise as a consequence, including the oxidation of guanine residues, leading to G?C-->T?A substitution, and oxidation of cytosine resulting in a G?C-->A?T substitution... - Estimation of the rate of unrecognized cross-contamination with mycobacterium tuberculosis in London microbiology laboratoriesM Ruddy
Department of Medical Microbiology, Royal Free and University College Medical School, London NW3 2PF, United Kingdom
J Clin Microbiol 40:4100-4. 2002..1 and 65%. These data indicate that laboratory cross-contamination has not been a common problem in routine practice in the London area, but in several incidents patients did receive full courses of therapy that were probably unnecessary... - Effect of subinhibitory concentrations of ciprofloxacin on Mycobacterium fortuitum mutation ratesStephen H Gillespie
Centre for Medical Microbiology, University College London, Hampstead Campus, Rowland Hill St, London NW3 2PF, UK
J Antimicrob Chemother 56:344-8. 2005.... - A practical guide to measuring mutation rates in antibiotic resistanceCassie F Pope
Centre for Medical Microbiology, Royal Free and University College Medical School, Rowland Hill Street, London NW3 2PF, United Kingdom
Antimicrob Agents Chemother 52:1209-14. 2008 - Studies of early bactericidal activity: new insights into isoniazid pharmacokineticsStephen H Gillespie
Clin Infect Dis 39:1431-2. 2004 - Evolutionary relationships among strains of Mycobacterium tuberculosis with few copies of IS6110Jeremy W Dale
School of Biomedical and Life Sciences, University of Surrey, Guildford, Surrey GU2 7XH, United Kingdom
J Bacteriol 185:2555-62. 2003..These lineages appear to be quite distinct from M. tuberculosis isolates with multiple copies of IS6110... - The bactericidal activity of moxifloxacin in patients with pulmonary tuberculosisRoly D Gosling
Department of Medical Microbiology, Royal Free and University London College Medical Schools, Royal Free Campus, London, UK
Am J Respir Crit Care Med 168:1342-5. 2003..Moxifloxacin has an activity similar to rifampin in human subjects with pulmonary tuberculosis, suggesting that it should undergo further assessment as part of a short course regimen for the treatment of drug-susceptible tuberculosis... - Direct detection of heteroresistance in Mycobacterium tuberculosis using molecular techniquesMairi M Cullen
J Med Microbiol 55:1157-8. 2006 - Origins and properties of Mycobacterium tuberculosis isolates in LondonJeremy W Dale
School of Biomedical and Molecular Sciences, University of Surrey, Guildford GU2 7XH, UK
J Med Microbiol 54:575-82. 2005.... - Impact of azithromycin administration for trachoma control on the carriage of antibiotic-resistant Streptococcus pneumoniaeSarah L Batt
Department of Medical Microbiology, University College London, Royal Free Campus, London NW3 2PF
Antimicrob Agents Chemother 47:2765-9. 2003..0001, Fisher's exact). These data suggest that in communities where macrolide resistance is rare, azithromycin distribution for trachoma control is unlikely to increase the prevalence of resistant organisms... - The ethics of non-inferiority trialsAndrew J Nunn
Lancet 371:895; author reply 896-7. 2008 - The bacteriology of pleural infection by genetic and standard methods and its mortality significanceNick A Maskell
Department of Respiratory Medicine, Southmead Hospital, North Bristol NHS Trust, UK
Am J Respir Crit Care Med 174:817-23. 2006..Antibiotics for community-acquired infection should treat aerobic and anaerobic bacteria. Hospital-acquired, gram-negative S. aureus and mixed aerobic infections have a high mortality rate...