S H Gillespie

Summary

Affiliation: University College London
Country: UK

Publications

  1. ncbi request reprint Early bactericidal activity of a moxifloxacin and isoniazid combination in smear-positive pulmonary tuberculosis
    Stephen H Gillespie
    Centre for Medical Microbiology, Hampstead Campus, Royal Free and University College Medical School, Rowland Hill Street, London NW3 2PF, UK
    J Antimicrob Chemother 56:1169-71. 2005
  2. ncbi request reprint Tuberculosis: evolution in millennia and minutes
    S H Gillespie
    Centre for Medical Microbiology, Hampstead Campus, Rowland Hill Street, London NW3 2PF, U K
    Biochem Soc Trans 35:1317-20. 2007
  3. doi request reprint Approaches to measure the fitness of Burkholderia cepacia complex isolates
    C F Pope
    Centre for Clinical Microbiology, University College London, Rowland Hill Street, London NW3 2QG, UK
    J Med Microbiol 59:679-86. 2010
  4. pmc Mycobacterium tuberculosis DNA repair in response to subinhibitory concentrations of ciprofloxacin
    D M O'Sullivan
    Centre for Medical Microbiology, Department of Infection, Royal Free Campus, University College London, Rowland Hill Street, Hampstead, London NW3 2PF, UK
    J Antimicrob Chemother 62:1199-202. 2008
  5. ncbi request reprint Mycobacterium tuberculosis lineage: a naming of the parts
    T D McHugh
    Department of Infection, Centre for Medical Microbiology, University College London, Royal Free Campus, Rowland Hill Street, Hampstead, London NW3 2PF, UK
    Tuberculosis (Edinb) 85:127-36. 2005
  6. ncbi request reprint A multicentre comparison of a novel surrogate marker for determining the specific potency of anti-tuberculosis drugs
    Roly D Gosling
    Department of Medical Microbiology, University College London, Royal Free Campus, Rowland Hill Street, London NW3 2PF, UK
    J Antimicrob Chemother 52:473-6. 2003
  7. doi request reprint The effect of oxidative stress on the mutation rate of Mycobacterium tuberculosis with impaired catalase/peroxidase function
    D M O'Sullivan
    Centre for Medical Microbiology, Department of Infection, Hampstead Campus, University College London, Rowland Hill Street, Hampstead, London NW3 2PF, UK
    J Antimicrob Chemother 62:709-12. 2008
  8. ncbi request reprint Clinical application of a rapid lung-orientated immunoassay in individuals with possible tuberculosis
    R A M Breen
    Department of Immunology, Royal Free and University College Medical School, London, UK
    Thorax 63:67-71. 2008
  9. ncbi request reprint The interpretation of nucleic acid amplification tests for tuberculosis: do rapid tests change treatment decisions?
    S J Conaty
    Department of Primary Care and Population Science, UCL Centre for Infectious Disease Epidemiology, Royal Free and University College Medical School, Royal Free Campus, London NW3 2PF, UK
    J Infect 50:187-92. 2005
  10. ncbi request reprint Antibiotic resistance in the absence of selective pressure
    S H Gillespie
    Royal Free and University College Medical School, Royal Free Campus, Rowland Hill Street, London NW3 2PF, UK
    Int J Antimicrob Agents 17:171-6. 2001

Collaborators

Detail Information

Publications37

  1. ncbi request reprint Early bactericidal activity of a moxifloxacin and isoniazid combination in smear-positive pulmonary tuberculosis
    Stephen H Gillespie
    Centre for Medical Microbiology, Hampstead Campus, Royal Free and University College Medical School, Rowland Hill Street, London NW3 2PF, UK
    J Antimicrob Chemother 56:1169-71. 2005
    ..In vitro and animal studies have shown that moxifloxacin-containing combinations may improve the bactericidal efficacy of antituberculosis regimens...
  2. ncbi request reprint Tuberculosis: evolution in millennia and minutes
    S H Gillespie
    Centre for Medical Microbiology, Hampstead Campus, Rowland Hill Street, London NW3 2PF, U K
    Biochem Soc Trans 35:1317-20. 2007
    ..M. tuberculosis is a highly effective pathogen that has caused disease in human populations for millennia. We are now starting to understand some of the genetic mechanisms behind this phenomenon...
  3. doi request reprint Approaches to measure the fitness of Burkholderia cepacia complex isolates
    C F Pope
    Centre for Clinical Microbiology, University College London, Rowland Hill Street, London NW3 2QG, UK
    J Med Microbiol 59:679-86. 2010
    ..Unlike in Pseudomonas aeruginosa where hypermutability has been detected in strains isolated from CF patients, we were unable to demonstrate hypermutability in this panel of Burkholderia cenocepacia and Burkholderia multivorans isolates...
  4. pmc Mycobacterium tuberculosis DNA repair in response to subinhibitory concentrations of ciprofloxacin
    D M O'Sullivan
    Centre for Medical Microbiology, Department of Infection, Royal Free Campus, University College London, Rowland Hill Street, Hampstead, London NW3 2PF, UK
    J Antimicrob Chemother 62:1199-202. 2008
    ..To investigate how the SOS response, an error-prone DNA repair pathway, is expressed following subinhibitory quinolone treatment of Mycobacterium tuberculosis...
  5. ncbi request reprint Mycobacterium tuberculosis lineage: a naming of the parts
    T D McHugh
    Department of Infection, Centre for Medical Microbiology, University College London, Royal Free Campus, Rowland Hill Street, Hampstead, London NW3 2PF, UK
    Tuberculosis (Edinb) 85:127-36. 2005
    ..Such a framework will permit associations between a lineage and clinical or bacterial phenomenon to be tested objectively. This definition will also enable new putative lineages to be objectively tested...
  6. ncbi request reprint A multicentre comparison of a novel surrogate marker for determining the specific potency of anti-tuberculosis drugs
    Roly D Gosling
    Department of Medical Microbiology, University College London, Royal Free Campus, Rowland Hill Street, London NW3 2PF, UK
    J Antimicrob Chemother 52:473-6. 2003
    ..From this, we conclude that the reiterative exponential decay model permits comparison between the data obtained in different centres and would allow the activity of a new drug to be compared with that of the currently available agents...
  7. doi request reprint The effect of oxidative stress on the mutation rate of Mycobacterium tuberculosis with impaired catalase/peroxidase function
    D M O'Sullivan
    Centre for Medical Microbiology, Department of Infection, Hampstead Campus, University College London, Rowland Hill Street, Hampstead, London NW3 2PF, UK
    J Antimicrob Chemother 62:709-12. 2008
    ..To determine the effect of oxidative stress on isoniazid-resistant Mycobacterium tuberculosis deficient in catalase/peroxidase activity to varying degrees through mutation in katG...
  8. ncbi request reprint Clinical application of a rapid lung-orientated immunoassay in individuals with possible tuberculosis
    R A M Breen
    Department of Immunology, Royal Free and University College Medical School, London, UK
    Thorax 63:67-71. 2008
    ..Lung sampling enables combined microbiological and immunological testing and uses higher frequency antigen-specific responses than in blood...
  9. ncbi request reprint The interpretation of nucleic acid amplification tests for tuberculosis: do rapid tests change treatment decisions?
    S J Conaty
    Department of Primary Care and Population Science, UCL Centre for Infectious Disease Epidemiology, Royal Free and University College Medical School, Royal Free Campus, London NW3 2PF, UK
    J Infect 50:187-92. 2005
    ..To describe changes in treatment decisions after receipt of nucleic acid amplification (NAA) test for the diagnosis of M. tuberculosis...
  10. ncbi request reprint Antibiotic resistance in the absence of selective pressure
    S H Gillespie
    Royal Free and University College Medical School, Royal Free Campus, Rowland Hill Street, London NW3 2PF, UK
    Int J Antimicrob Agents 17:171-6. 2001
    ..Ultimately, we must look again to new drug discovery to improve our therapeutic armoury...
  11. ncbi request reprint Prospective evaluation of BDProbeTec strand displacement amplification (SDA) system for diagnosis of tuberculosis in non-respiratory and respiratory samples
    T D McHugh
    Centre for Medical Microbiology, Department of Infection, Royal Free and University College Medical School, Pond Street, London NW3 2PF, UK
    J Med Microbiol 53:1215-9. 2004
    ..Specimen type did not predict likelihood of a sample being in the low-positive zone. Although the manufacturers do not describe the concept of a low-positive zone, we have found that it aids clinical diagnosis...
  12. ncbi request reprint A novel method for evaluating the antimicrobial activity of tuberculosis treatment regimens
    S H Gillespie
    Department of Medical Microbiology, University College London, London, United Kingdom
    Int J Tuberc Lung Dis 7:684-9. 2003
    ..To evaluate the clinical response to antituberculosis chemotherapy rapidly...
  13. ncbi request reprint A comparison of the bactericidal activity of quinolone antibiotics in a Mycobacterium fortuitum model
    S H Gillespie
    Royal Free and University College Medical School, London
    J Med Microbiol 50:565-70. 2001
    ..These data indicate that moxifloxacin was the most bactericidal of the fluoroquinolones tested. Such methods provide a simple in-vitro measure that correlates with in-vivo models...
  14. ncbi request reprint Evolutionary barriers to quinolone resistance in Streptococcus pneumoniae
    Stephen H Gillespie
    Department of Medical Microbiology, Royal Free and University College Medical School, London, UK
    Microb Drug Resist 8:79-84. 2002
    ..16 (95% C.I. +/- 0.17), 0.99 (95% C.I. +/- 0.05), and 0.95 (95% C.I. +/- 0.05), respectively. These data suggest that mutation in the parC and gyrA genes may, on some occasions, not be associated with a physiological deficit...
  15. pmc Novel PCR-restriction fragment length polymorphism method for determining serotypes or serogroups of Streptococcus pneumoniae isolates
    Sarah L Batt
    Centre for Medical Microbiology, University College London, Royal Free Campus, Rowland Hill St, London NW3 2PF, United Kingdom
    J Clin Microbiol 43:2656-61. 2005
    ..Once our database contains all 90 serotypes, this technique should be fully portable, cost-effective, and useful in any laboratory with sufficient molecular experience...
  16. ncbi request reprint A reiterative method for calculating the early bactericidal activity of antituberculosis drugs
    Stephen H Gillespie
    Academic Department of Medical Microbiology, Royal Free and University College Medical School, London, United Kingdom
    Am J Respir Crit Care Med 166:31-5. 2002
    ....
  17. ncbi request reprint Microbes sans frontières
    Stephen H Gillespie
    Department of Medical Microbiology, University College London, Royal Free Campus, NW3 2PF, London, UK
    Lancet 359:93-5. 2002
  18. ncbi request reprint Multiple drug-resistant Mycobacterium tuberculosis: evidence for changing fitness following passage through human hosts
    Stephen H Gillespie
    Department of Medical Microbiology, Royal Free and University College Medical School, London NW3 2PF, UK
    Microb Drug Resist 8:273-9. 2002
    ..This study provides the first evidence that multiple drug resistant M. tuberculosis strains adapt to the environment of their human host...
  19. ncbi request reprint Fluoroquinolone resistance in Streptococcus pneumoniae: evidence that gyrA mutations arise at a lower rate and that mutation in gyrA or parC predisposes to further mutation
    Stephen H Gillespie
    Department of Medical Microbiology, Royal Free and University College Medical School, London NW3 2PF, UK
    Microb Drug Resist 9:17-24. 2003
    ..3 x 10(-8) for parC Serine 79 Tyrosine and 7.2 x 10(-9) for gyrA Serine 81 Phenylalanine, 12 and 450 times higher, respectively, than for first-step rates, suggesting that mutation in either gene readies the genome for further mutation...
  20. pmc Evolution of drug resistance in Mycobacterium tuberculosis: clinical and molecular perspective
    Stephen H Gillespie
    Royal Free and University College Medical School, University College London, London NW3 2PF, United Kingdom
    Antimicrob Agents Chemother 46:267-74. 2002
  21. ncbi request reprint New tricks from an old dog: streptococcal necrotising soft-tissue infections
    Stephen H Gillespie
    University College London, Royal Free Campus, London NW3 2PF, UK
    Lancet 363:672-3. 2004
  22. ncbi request reprint Biomarkers of treatment response in clinical trials of novel antituberculosis agents
    Felicity M R Perrin
    Centre of Medical Microbiology, Royal Free and University College Medical School, London, UK
    Lancet Infect Dis 7:481-90. 2007
    ..Finally, we propose methods to assess candidate markers, and how these candidate markers could be implemented in future clinical trials...
  23. doi request reprint Resuscitation-promoting factors are expressed in Mycobacterium tuberculosis-infected human tissue
    A P Davies
    Centre for Medical Microbiology, Department of Infection, Royal Free and University College Medical School, London, UK
    Tuberculosis (Edinb) 88:462-8. 2008
    ..The work presented here demonstrates that Rpf expression can be detected in human tissues infected with M. tuberculosis and describes the pattern of this expression using immunocytochemistry with anti-Rpf antibodies...
  24. pmc Fluoroquinolone-resistant mutants of Burkholderia cepacia
    C F Pope
    Centre for Medical Microbiology, Royal Free and University College Medical School, Rowland Hill Street, London NW3 2PF, United Kingdom
    Antimicrob Agents Chemother 52:1201-3. 2008
    ..Growth rate, biofilm formation, and survival in water and during drying were not impaired in strains containing single gyrA mutations. Double mutants were impaired only in growth rate (0.85, relative to the susceptible parent)...
  25. ncbi request reprint Think TB! Is the diagnosis of pulmonary tuberculosis delayed by the use of antibiotics?
    S E Craig
    Department of Respiratory Medicine, Royal Free Hospital, and Department of Medical Statistics and Epidemiology, Royal Free and University College Medical School, London, UK
    Int J Tuberc Lung Dis 13:208-13. 2009
    ..Effective tuberculosis (TB) control requires prompt diagnosis of infectious cases through early suspicion of pulmonary TB in all subjects with suspected respiratory infection...
  26. ncbi request reprint Analysis of rpoB and pncA mutations in the published literature: an insight into the role of oxidative stress in Mycobacterium tuberculosis evolution?
    Denise M O'Sullivan
    Centre for Medical Microbiology, Department of Infection, Royal Free and University College Medical School, Royal Free Campus, University College London, Rowland Hill Street, London NW3 2PF, UK
    J Antimicrob Chemother 55:674-9. 2005
    ..Various mutations are thought to arise as a consequence, including the oxidation of guanine residues, leading to G?C-->T?A substitution, and oxidation of cytosine resulting in a G?C-->A?T substitution...
  27. pmc Estimation of the rate of unrecognized cross-contamination with mycobacterium tuberculosis in London microbiology laboratories
    M Ruddy
    Department of Medical Microbiology, Royal Free and University College Medical School, London NW3 2PF, United Kingdom
    J Clin Microbiol 40:4100-4. 2002
    ..1 and 65%. These data indicate that laboratory cross-contamination has not been a common problem in routine practice in the London area, but in several incidents patients did receive full courses of therapy that were probably unnecessary...
  28. ncbi request reprint Effect of subinhibitory concentrations of ciprofloxacin on Mycobacterium fortuitum mutation rates
    Stephen H Gillespie
    Centre for Medical Microbiology, University College London, Hampstead Campus, Rowland Hill St, London NW3 2PF, UK
    J Antimicrob Chemother 56:344-8. 2005
    ..The purpose of this study is to extend this observation to mycobacteria and to quantify mutation rates...
  29. pmc A practical guide to measuring mutation rates in antibiotic resistance
    Cassie F Pope
    Centre for Medical Microbiology, Royal Free and University College Medical School, Rowland Hill Street, London NW3 2PF, United Kingdom
    Antimicrob Agents Chemother 52:1209-14. 2008
  30. ncbi request reprint Studies of early bactericidal activity: new insights into isoniazid pharmacokinetics
    Stephen H Gillespie
    Clin Infect Dis 39:1431-2. 2004
  31. pmc Evolutionary relationships among strains of Mycobacterium tuberculosis with few copies of IS6110
    Jeremy W Dale
    School of Biomedical and Life Sciences, University of Surrey, Guildford, Surrey GU2 7XH, United Kingdom
    J Bacteriol 185:2555-62. 2003
    ..These lineages appear to be quite distinct from M. tuberculosis isolates with multiple copies of IS6110...
  32. ncbi request reprint The bactericidal activity of moxifloxacin in patients with pulmonary tuberculosis
    Roly D Gosling
    Department of Medical Microbiology, Royal Free and University London College Medical Schools, Royal Free Campus, London, UK
    Am J Respir Crit Care Med 168:1342-5. 2003
    ..Moxifloxacin has an activity similar to rifampin in human subjects with pulmonary tuberculosis, suggesting that it should undergo further assessment as part of a short course regimen for the treatment of drug-susceptible tuberculosis...
  33. ncbi request reprint Direct detection of heteroresistance in Mycobacterium tuberculosis using molecular techniques
    Mairi M Cullen
    J Med Microbiol 55:1157-8. 2006
  34. ncbi request reprint Origins and properties of Mycobacterium tuberculosis isolates in London
    Jeremy W Dale
    School of Biomedical and Molecular Sciences, University of Surrey, Guildford GU2 7XH, UK
    J Med Microbiol 54:575-82. 2005
    ....
  35. pmc Impact of azithromycin administration for trachoma control on the carriage of antibiotic-resistant Streptococcus pneumoniae
    Sarah L Batt
    Department of Medical Microbiology, University College London, Royal Free Campus, London NW3 2PF
    Antimicrob Agents Chemother 47:2765-9. 2003
    ..0001, Fisher's exact). These data suggest that in communities where macrolide resistance is rare, azithromycin distribution for trachoma control is unlikely to increase the prevalence of resistant organisms...
  36. doi request reprint The ethics of non-inferiority trials
    Andrew J Nunn
    Lancet 371:895; author reply 896-7. 2008
  37. ncbi request reprint The bacteriology of pleural infection by genetic and standard methods and its mortality significance
    Nick A Maskell
    Department of Respiratory Medicine, Southmead Hospital, North Bristol NHS Trust, UK
    Am J Respir Crit Care Med 174:817-23. 2006
    ..Antibiotic choices for pleural infection are uncertain as its bacteriology is poorly described...