A Geerlof

Summary

Affiliation: University of Leicester
Country: UK

Publications

  1. ncbi Purification and characterization of phosphopantetheine adenylyltransferase from Escherichia coli
    A Geerlof
    Department of Microbiology and Immunology, University of Leicester, Leicester LE1 9HN, United Kingdom
    J Biol Chem 274:27105-11. 1999
  2. pmc The crystal structure of a novel bacterial adenylyltransferase reveals half of sites reactivity
    T Izard
    Department of Biochemistry, University of Leicester, Leicester LE1
    EMBO J 18:2021-30. 1999
  3. ncbi Cubic crystals of phosphopantetheine adenylyltransferase from Escherichia coli
    T Izard
    Department of Biochemistry, University of Leicester, Leicester LE1 7RH, England
    Acta Crystallogr D Biol Crystallogr 55:1226-8. 1999
  4. ncbi Implementation of semi-automated cloning and prokaryotic expression screening: the impact of SPINE
    Pedro M Alzari
    Unité de Biochimie Structurale, Institut Pasteur, 25 28 Rue du Dr Roux, Paris Cedex 15, France
    Acta Crystallogr D Biol Crystallogr 62:1103-13. 2006

Collaborators

  • T Izard
  • Pedro M Alzari
  • M J Fogg
  • N S Berrow
  • V Campanacci
  • M P A Luna-Vargas
  • S Macieira
  • S Eiler
  • P Nordlund
  • M Willmanns
  • M Velarde
  • A Perrakis
  • M D Herman
  • H van Tilbeurgh
  • E Christodoulou
  • D Hart
  • C Cambillau
  • A Haouz
  • E Blagova
  • Raymond J Owens
  • T Unger
  • H Berglund
  • S Quevillon-Cheruel
  • D Busso
  • F Tarandeau
  • G Folkers

Detail Information

Publications4

  1. ncbi Purification and characterization of phosphopantetheine adenylyltransferase from Escherichia coli
    A Geerlof
    Department of Microbiology and Immunology, University of Leicester, Leicester LE1 9HN, United Kingdom
    J Biol Chem 274:27105-11. 1999
    ..Since purified PPAT lacks dephospho-CoA kinase activity, the two final steps of CoA biosynthesis in E. coli must be catalyzed by separate enzymes...
  2. pmc The crystal structure of a novel bacterial adenylyltransferase reveals half of sites reactivity
    T Izard
    Department of Biochemistry, University of Leicester, Leicester LE1
    EMBO J 18:2021-30. 1999
    ..The homologous active site attachment of ATP and the structural distribution of predicted sequence-binding motifs in PPAT classify the enzyme as belonging to the nucleotidyltransferase superfamily...
  3. ncbi Cubic crystals of phosphopantetheine adenylyltransferase from Escherichia coli
    T Izard
    Department of Biochemistry, University of Leicester, Leicester LE1 7RH, England
    Acta Crystallogr D Biol Crystallogr 55:1226-8. 1999
    ..5 A. The crystals diffract to better than 1.8 A resolution on a Cu Kalpha rotating-anode generator. The asymmetric unit is likely to contain two molecules, corresponding to a packing density of 2.9 A3 Da-1...
  4. ncbi Implementation of semi-automated cloning and prokaryotic expression screening: the impact of SPINE
    Pedro M Alzari
    Unité de Biochimie Structurale, Institut Pasteur, 25 28 Rue du Dr Roux, Paris Cedex 15, France
    Acta Crystallogr D Biol Crystallogr 62:1103-13. 2006
    ..In addition to the implementation of HTP cloning and expression screening, the development of two novel technologies is described, namely library-based screening for soluble constructs and parallel small-scale high-density fermentation...