Andrew M Fry

Summary

Affiliation: University of Leicester
Country: UK

Publications

  1. ncbi request reprint Structure and regulation of the human Nek2 centrosomal kinase
    Peter Rellos
    Structural Genomics Consortium, Botnar Research Centre, University of Oxford, Oxford OX3 7LD, United Kingdom
    J Biol Chem 282:6833-42. 2007
  2. pmc An undecided coiled coil: the leucine zipper of Nek2 kinase exhibits atypical conformational exchange dynamics
    Rebecca Croasdale
    Department of Biochemistry, University of Leicester, Leicester LE1 9HN, United Kingdom
    J Biol Chem 286:27537-47. 2011
  3. pmc The Nek8 protein kinase, mutated in the human cystic kidney disease nephronophthisis, is both activated and degraded during ciliogenesis
    Detina Zalli
    Department of Biochemistry, University of Leicester, Lancaster Road, Leicester LE1 9HN, UK
    Hum Mol Genet 21:1155-71. 2012
  4. pmc Cell cycle regulation by the NEK family of protein kinases
    Andrew M Fry
    Department of Biochemistry, University of Leicester, Lancaster Road, Leicester LE1 9HN, UK
    J Cell Sci 125:4423-33. 2012
  5. ncbi request reprint APC/C-mediated degradation in early mitosis: how to avoid spindle assembly checkpoint inhibition
    Andrew M Fry
    Department of Biochemistry, University of Leicester, Leicester, UK
    Cell Cycle 5:1487-91. 2006
  6. doi request reprint Under arrest in mitosis: Cdc20 dies twice
    Andrew M Fry
    Department of Biochemistry, University of Leicester, Leicester LE1 9HN, UK
    Nat Cell Biol 10:1385-7. 2008
  7. pmc Mitotic regulation by NIMA-related kinases
    Laura O'regan
    Department of Biochemistry, University of Leicester, Leicester, UK
    Cell Div 2:25. 2007
  8. ncbi request reprint The Nek2 protein kinase: a novel regulator of centrosome structure
    Andrew M Fry
    Department of Biochemistry, University of Leicester, Leicester LE1 7RH, UK
    Oncogene 21:6184-94. 2002
  9. ncbi request reprint The NIMA-related kinase X-Nek2B is required for efficient assembly of the zygotic centrosome in Xenopus laevis
    A M Fry
    Department of Biochemistry, University of Leicester, Adrian Building, University Road, Leicester LE1 7RH, UK
    J Cell Sci 113:1973-84. 2000
  10. ncbi request reprint Activity of the human centrosomal kinase, Nek2, depends on an unusual leucine zipper dimerization motif
    A M Fry
    Department of Molecular Biology, Sciences II, University of Geneva, 30, Quai Ernest Ansermet, CH 1211 Geneva 4, Switzerland
    J Biol Chem 274:16304-10. 1999

Collaborators

Detail Information

Publications40

  1. ncbi request reprint Structure and regulation of the human Nek2 centrosomal kinase
    Peter Rellos
    Structural Genomics Consortium, Botnar Research Centre, University of Oxford, Oxford OX3 7LD, United Kingdom
    J Biol Chem 282:6833-42. 2007
    ....
  2. pmc An undecided coiled coil: the leucine zipper of Nek2 kinase exhibits atypical conformational exchange dynamics
    Rebecca Croasdale
    Department of Biochemistry, University of Leicester, Leicester LE1 9HN, United Kingdom
    J Biol Chem 286:27537-47. 2011
    ..As a result, the leucine zipper of Nek2 kinase is the first example where the frameshift of coiled-coil heptad repeats has been directly observed experimentally...
  3. pmc The Nek8 protein kinase, mutated in the human cystic kidney disease nephronophthisis, is both activated and degraded during ciliogenesis
    Detina Zalli
    Department of Biochemistry, University of Leicester, Lancaster Road, Leicester LE1 9HN, UK
    Hum Mol Genet 21:1155-71. 2012
    ..Taken together, these findings reveal important insights into the mechanisms through which Nek8 activity and localization are regulated during ciliogenesis...
  4. pmc Cell cycle regulation by the NEK family of protein kinases
    Andrew M Fry
    Department of Biochemistry, University of Leicester, Lancaster Road, Leicester LE1 9HN, UK
    J Cell Sci 125:4423-33. 2012
    ..Here, we review the functions of the human NEKs, with particular emphasis on those family members that are involved in cell cycle control, and consider their potential as therapeutic targets in cancer...
  5. ncbi request reprint APC/C-mediated degradation in early mitosis: how to avoid spindle assembly checkpoint inhibition
    Andrew M Fry
    Department of Biochemistry, University of Leicester, Leicester, UK
    Cell Cycle 5:1487-91. 2006
    ..The direct interaction of substrates with core subunits of the APC/C could allow their degradation to proceed unhindered even when the SAC is active...
  6. doi request reprint Under arrest in mitosis: Cdc20 dies twice
    Andrew M Fry
    Department of Biochemistry, University of Leicester, Leicester LE1 9HN, UK
    Nat Cell Biol 10:1385-7. 2008
    ..Degradation of the APC/C activator Cdc20 seems to be an essential and conserved mechanism to maintain this checkpoint in the presence of chromosomes that are not attached to the mitotic spindle...
  7. pmc Mitotic regulation by NIMA-related kinases
    Laura O'regan
    Department of Biochemistry, University of Leicester, Leicester, UK
    Cell Div 2:25. 2007
    ..Furthermore, we highlight their potential as novel chemotherapeutic targets...
  8. ncbi request reprint The Nek2 protein kinase: a novel regulator of centrosome structure
    Andrew M Fry
    Department of Biochemistry, University of Leicester, Leicester LE1 7RH, UK
    Oncogene 21:6184-94. 2002
    ..Recent studies in lower organisms have raised the possibility that kinases related to Nek2 may have conserved functions in MTOC organization, as well as in other aspects of mitotic progression...
  9. ncbi request reprint The NIMA-related kinase X-Nek2B is required for efficient assembly of the zygotic centrosome in Xenopus laevis
    A M Fry
    Department of Biochemistry, University of Leicester, Adrian Building, University Road, Leicester LE1 7RH, UK
    J Cell Sci 113:1973-84. 2000
    ..Hence, these studies demonstrate that X-Nek2B is required for efficient assembly of a functional zygotic centrosome and highlight the possibility of multiple roles for vertebrate Nek2 kinases in the centrosome cycle...
  10. ncbi request reprint Activity of the human centrosomal kinase, Nek2, depends on an unusual leucine zipper dimerization motif
    A M Fry
    Department of Molecular Biology, Sciences II, University of Geneva, 30, Quai Ernest Ansermet, CH 1211 Geneva 4, Switzerland
    J Biol Chem 274:16304-10. 1999
    ..Thus, this study reveals not only an important mechanism for the regulation of the Nek2 kinase but, furthermore, highlights an unusual organization of a leucine zipper dimerization motif...
  11. pmc Coordinate regulation of the mother centriole component nlp by nek2 and plk1 protein kinases
    Joseph Rapley
    Department of Biochemistry, University of Leicester, University Rd, Leicester LE1 7RH, United Kingdom
    Mol Cell Biol 25:1309-24. 2005
    ..We also propose that phosphorylation by Nek2 may prime Nlp for phosphorylation by Plk1...
  12. ncbi request reprint Pix1 and Pix2 are novel WD40 microtubule-associated proteins that colocalize with mitochondria in Xenopus germ plasm and centrosomes in human cells
    Rebecca S Hames
    Department of Biochemistry, University of Leicester, University Road, Leicester LE1 9HN, UK
    Exp Cell Res 314:574-89. 2008
    ..Based on these data, we propose that Pix1 and Pix2 are microtubule-associated adaptor proteins that likely contribute to a range of developmental and cell division processes...
  13. pmc The Nek6 and Nek7 protein kinases are required for robust mitotic spindle formation and cytokinesis
    Laura O'regan
    Department of Biochemistry, University of Leicester, Lancaster Road, Leicester LE1 9HN, United Kingdom
    Mol Cell Biol 29:3975-90. 2009
    ..Together, these data lead us to propose that Nek6 and Nek7 play independent roles not only in robust mitotic spindle formation but also potentially in cytokinesis...
  14. pmc Dynamic recruitment of Nek2 kinase to the centrosome involves microtubules, PCM-1, and localized proteasomal degradation
    Rebecca S Hames
    Department of Biochemistry, University of Leicester, UK
    Mol Biol Cell 16:1711-24. 2005
    ..Together, these data highlight multiple processes involved in regulating the abundance of Nek2 kinase at the centrosome including microtubule binding, the centriolar satellite component PCM-1, and localized protein degradation...
  15. pmc Poc1A and Poc1B act together in human cells to ensure centriole integrity
    Magali Venoux
    Department of Biochemistry, University of Leicester, Lancaster Road, Leicester LE1 9HN, UK
    J Cell Sci 126:163-75. 2013
    ..Hence, Poc1A and Poc1B play redundant, but essential, roles in generation of stable centrioles, but Poc1B may have additional independent functions during cell cycle progression...
  16. pmc Molecular dissection of the centrosome overduplication pathway in S-phase-arrested cells
    Suzanna L Prosser
    Department of Biochemistry, University of Leicester, Leicester LE1 9HN, United Kingdom
    Mol Cell Biol 29:1760-73. 2009
    ..Furthermore, this study defines a molecular pathway that may be targeted to prevent centrosome overduplication in S-phase-arrested cancer cells...
  17. ncbi request reprint Early mitotic degradation of Nek2A depends on Cdc20-independent interaction with the APC/C
    Michelle J Hayes
    Department of Biochemistry, University of Leicester, Leicester, Leics LE1 7RH, UK
    Nat Cell Biol 8:607-14. 2006
    ..Thus, although Cdc20 is required for the degradation of Nek2A, it is not required for its recruitment and this renders its degradation insensitive to the mitotic checkpoint...
  18. ncbi request reprint Oscillation of APC/C activity during cell cycle arrest promotes centrosome amplification
    Suzanna L Prosser
    Department of Biochemistry, University of Leicester, Lancaster Road, Leicester LE1 9HN, UK
    J Cell Sci 125:5353-68. 2012
    ..Thus, oscillation of APC/C activity during cell cycle arrest promotes both centrosome amplification and genome instability...
  19. ncbi request reprint Sealed with a Kiz: How Plk1 ensures spindle pole integrity
    Andrew M Fry
    Department of Biochemistry, University of Leicester, Lancaster Road, Leicester LE1 9HN, United Kingdom
    Dev Cell 11:431-2. 2006
    ..2006). Phosphorylation of Kiz ensures the integrity of spindle poles in the face of severe pulling forces exerted by the chromosome-attached spindle microtubules...
  20. pmc SUN1 interacts with nuclear lamin A and cytoplasmic nesprins to provide a physical connection between the nuclear lamina and the cytoskeleton
    Farhana Haque
    Department of Genetics, University of Leicester, University Road, Leicester LE1 7RH, United Kingdom
    Mol Cell Biol 26:3738-51. 2006
    ..Our data provide evidence of a physical nuclear-cytoskeletal connection that is likely to be a key mechanism in nuclear-cytoplasmic communication and regulation of nuclear position...
  21. ncbi request reprint Nek2 kinase in chromosome instability and cancer
    Daniel G Hayward
    Department of Biochemistry, University of Leicester, Adrian Building, University Road, Leicester LE1 7RH, UK
    Cancer Lett 237:155-66. 2006
    ....
  22. pmc Nek2A kinase stimulates centrosome disjunction and is required for formation of bipolar mitotic spindles
    Alison J Faragher
    Department of Biochemistry, University of Leicester, Leicester LE1 7RH, United Kingdom
    Mol Biol Cell 14:2876-89. 2003
    ..However, they suggest that loss of centriole cohesion and C-Nap1 displacement may be distinct mitotic events...
  23. pmc Alternative splice variants of the human centrosome kinase Nek2 exhibit distinct patterns of expression in mitosis
    Rebecca S Hames
    Department of Biochemistry, University of Leicester, University Road, Leicester LE1 7RH, U K
    Biochem J 361:77-85. 2002
    ..This has important implications for our understanding of both Nek2 protein kinase regulation and the control of centrosome structure during mitosis...
  24. doi request reprint Fluorescence imaging of the centrosome cycle in mammalian cells
    Suzanna L Prosser
    Department of Biochemistry, University of Leicester, Leicester, UK
    Methods Mol Biol 545:165-83. 2009
    ....
  25. ncbi request reprint Nek2B stimulates zygotic centrosome assembly in Xenopus laevis in a kinase-independent manner
    Ciara Twomey
    Department of Biochemistry, University of Leicester, University Road, Leicester LE1 7RH, UK
    Dev Biol 265:384-98. 2004
    ..These results support a model in which maternal Nek2B acts to promote assembly of a functional zygotic centrosome in a kinase-independent manner...
  26. pmc Centriolar satellites are assembly points for proteins implicated in human ciliopathies, including oral-facial-digital syndrome 1
    Carla A M Lopes
    Department of Biochemistry, University of Leicester, Lancaster Road, Leicester LE1 9HN, UK
    J Cell Sci 124:600-12. 2011
    ..Furthermore, definition of how OFD1 and PCM-1 interact helps explain why different OFD1 mutations lead to clinically variable phenotypes...
  27. pmc A centrosomal function for the human Nek2 protein kinase, a member of the NIMA family of cell cycle regulators
    A M Fry
    Department of Molecular Biology, Sciences II, University of Geneva, Switzerland
    EMBO J 17:470-81. 1998
    ..Moreover, they indicate that one function of mammalian Nek2 relates to the centrosome cycle and thus provide a new perspective on the role of NIMA-related kinases...
  28. ncbi request reprint Multisite phosphorylation of C-Nap1 releases it from Cep135 to trigger centrosome disjunction
    Tara Hardy
    Department of Biochemistry, University of Leicester, Lancaster Road, Leicester LE1 9HN, UK
    J Cell Sci 127:2493-506. 2014
    ..We therefore propose that multisite phosphorylation of C-Nap1 by Nek2 perturbs both oligomerization and Cep135 interaction, and this precipitates centrosome disjunction at the onset of mitosis. ..
  29. ncbi request reprint Crystal structure of EML1 reveals the basis for Hsp90 dependence of oncogenic EML4-ALK by disruption of an atypical β-propeller domain
    Mark W Richards
    Department of Biochemistry, University of Leicester, Leicester LE1 9HN, United Kingdom
    Proc Natl Acad Sci U S A 111:5195-200. 2014
    ..The Hsp90-sensitive EML4-ALK variants are exceptions to the rule that oncogenic fusion proteins involve breakpoints in disordered regions of both partners. ..
  30. ncbi request reprint The centrosomal kinase Nek2 displays elevated levels of protein expression in human breast cancer
    Daniel G Hayward
    Department of Biochemistry, University of Leicester, Leicester, USA
    Cancer Res 64:7370-6. 2004
    ..These data highlight the Nek2 kinase as novel potential target for chemotherapeutic intervention in breast cancer...
  31. ncbi request reprint Identification by high-throughput screening of viridin analogs as biochemical and cell-based inhibitors of the cell cycle-regulated nek2 kinase
    Daniel G Hayward
    Department of Biochemistry, University of Leicester, Leicester, UK
    J Biomol Screen 15:918-27. 2010
    ....
  32. pmc APC/C-mediated destruction of the centrosomal kinase Nek2A occurs in early mitosis and depends upon a cyclin A-type D-box
    R S Hames
    Department of Biochemistry, University of Leicester, Leicester LE1 7RH, UK
    EMBO J 20:7117-27. 2001
    ..We propose that recognition of substrates by the APC/C-Cdc20 in early mitosis depends upon possession of an extended D-box motif...
  33. pmc C-Nap1, a novel centrosomal coiled-coil protein and candidate substrate of the cell cycle-regulated protein kinase Nek2
    A M Fry
    Department of Molecular Biology, Sciences II, University of Geneva, CH 1211 Geneva 4, Switzerland
    J Cell Biol 141:1563-74. 1998
    ..Based on these findings, we propose a model implicating both Nek2 and C-Nap1 in the regulation of centriole-centriole cohesion during the cell cycle...
  34. pmc The centrosomal protein C-Nap1 is required for cell cycle-regulated centrosome cohesion
    T Mayor
    Department of Molecular Biology, Sciences II, University of Geneva, CH 1211 Geneva, Switzerland
    J Cell Biol 151:837-46. 2000
    ..We conclude that C-Nap1 is a key component of a dynamic, cell cycle-regulated structure that mediates centriole-centriole cohesion...
  35. ncbi request reprint Histone deacetylase (HDAC) 1 and 2 are essential for accurate cell division and the pluripotency of embryonic stem cells
    Shereen Jamaladdin
    Department of Biochemistry, University of Leicester, Leicester LE1 9HN, United Kingdom and
    Proc Natl Acad Sci U S A 111:9840-5. 2014
    ..Our data indicate that HDAC1/2 have essential and pleiotropic roles in cellular proliferation and regulate stem cell self-renewal by maintaining expression of key pluripotent transcription factors. ..
  36. ncbi request reprint Protein kinases in control of the centrosome cycle
    T Mayor
    Department of Molecular Biology, University of Geneva, Switzerland
    FEBS Lett 452:92-5. 1999
    ..Here, we focus on two protein kinases, the polo-like kinase 1 and the NIMA-related kinase 2, for which recent data indicate key roles during the centrosome cycle...
  37. ncbi request reprint Polo-like kinase-2 is required for centriole duplication in mammalian cells
    Silke Warnke
    Cell Cycle Control and Carcinogenesis, F045, German Cancer Research Center DKFZ, Im Neuenheimer Feld 242, D 69120 Heidelberg, Germany
    Curr Biol 14:1200-7. 2004
    ..These results show that Plk2 is a physiological centrosomal protein and that its kinase activity is likely to be required for centriole duplication near the G1-to-S phase transition...
  38. pmc Xenopus polo-like kinase Plx1 regulates XErp1, a novel inhibitor of APC/C activity
    Andreas Schmidt
    Chemical Biology, Independent Research Group, Max Planck Institute of Biochemistry, 82152 Martinsried, Germany
    Genes Dev 19:502-13. 2005
    ..Plx1 phosphorylates XErp1 in vitro at a site that targets XErp1 for degradation upon CSF release. Thus, our data lead to a model of APC/C activation in Xenopus egg extract in which Plx1 targets the APC/C inhibitor XErp1 for degradation...
  39. ncbi request reprint Alternative splicing controls nuclear translocation of the cell cycle-regulated Nek2 kinase
    Wenjuan Wu
    Laboratório de Transdução de Sinais, Centro de Biologia Celular, Universidade de Aveiro, 3810 193 Aveiro, Portugal
    J Biol Chem 282:26431-40. 2007
    ..Thus, alternative splicing provides an unusual mechanism for modulating Nek2 localization, enabling it to have both nuclear and cytoplasmic functions...
  40. ncbi request reprint OFD1 is a centrosomal/basal body protein expressed during mesenchymal-epithelial transition in human nephrogenesis
    Leila Romio
    Nephro Urology Unit, Institute of Child Health, University College London, WC1N 1EH, UK
    J Am Soc Nephrol 15:2556-68. 2004
    ....