John S Fletcher

Summary

Affiliation: University of Manchester
Country: UK

Publications

  1. ncbi request reprint TOF-SIMS 3D biomolecular imaging of Xenopus laevis oocytes using buckminsterfullerene (C60) primary ions
    John S Fletcher
    Manchester Interdisciplinary Biocentre, Centre for Instrumentation and Analytical Science, School of Chemical Engineering and Analytical Science, The University of Manchester, Manchester, M1 7ND, UK John Fletcher manchester ac uk
    Anal Chem 79:2199-206. 2007
  2. ncbi request reprint TOF-SIMS analysis using C60. Effect of impact energy on yield and damage
    John S Fletcher
    Surface Analysis Research Centre, School of Chemical Engineering and Analytical Science, The University of Manchester, Manchester M60 1QD, UK
    Anal Chem 78:1827-31. 2006
  3. pmc Label free biochemical 2D and 3D imaging using secondary ion mass spectrometry
    John S Fletcher
    School of Chemical Engineering and Analytical Science, Manchester Interdisciplinary Biocentre, University of Manchester, Manchester M1 7DN, UK
    Curr Opin Chem Biol 15:733-40. 2011
  4. doi request reprint Three-dimensional mass spectral imaging of HeLa-M cells--sample preparation, data interpretation and visualisation
    John S Fletcher
    Manchester Interdisciplinary Biocentre, School of Chemical Engineering and Analytical Science, University of Manchester, 131 Princess Street, Manchester M1 7DN, UK
    Rapid Commun Mass Spectrom 25:925-32. 2011
  5. doi request reprint A new dynamic in mass spectral imaging of single biological cells
    John S Fletcher
    Manchester Interdisciplinary Biocentre, School of Chemical Engineering and Analytical Science, University of Manchester, Manchester, UK
    Anal Chem 80:9058-64. 2008
  6. ncbi request reprint Cellular imaging with secondary ion mass spectrometry
    John S Fletcher
    Manchester Interdisciplinary Biocentre, University of Manchester, Manchester, UK
    Analyst 134:2204-15. 2009
  7. ncbi request reprint Explanatory multivariate analysis of ToF-SIMS spectra for the discrimination of bacterial isolates
    Seetharaman Vaidyanathan
    School of Chemical Engineering and Analytical Science, Manchester Interdisciplinary Biocentre, University of Manchester, 131 Princess Street, Manchester, UK M1 7ND
    Analyst 134:2352-60. 2009
  8. doi request reprint Effects of cryogenic sample analysis on molecular depth profiles with TOF-secondary ion mass spectrometry
    Alan M Piwowar
    Surface Analysis Research Centre, Manchester Interdisciplinary Biocentre, School of Chemical Engineering and Analytical Science, The University of Manchester, Manchester M13 9PL, United Kingdom
    Anal Chem 82:8291-9. 2010
  9. doi request reprint TOF-SIMS with argon gas cluster ion beams: a comparison with C60+
    Sadia Rabbani
    Manchester Interdisciplinary Biocentre, School of Chemical Engineering and Analytical Science, University of Manchester, 131 Princess Street, Manchester, M13 9PL, UK
    Anal Chem 83:3793-800. 2011
  10. doi request reprint Subsurface biomolecular imaging of Streptomyces coelicolor using secondary ion mass spectrometry
    Seetharaman Vaidyanathan
    School of Chemical Engineering and Analytical Sciences, and School of Chemistry, Manchester Interdisciplinary Biocentre, The University of Manchester, 131 Princess Street, Manchester M1 7DN, UK
    Anal Chem 80:1942-51. 2008

Collaborators

Detail Information

Publications15

  1. ncbi request reprint TOF-SIMS 3D biomolecular imaging of Xenopus laevis oocytes using buckminsterfullerene (C60) primary ions
    John S Fletcher
    Manchester Interdisciplinary Biocentre, Centre for Instrumentation and Analytical Science, School of Chemical Engineering and Analytical Science, The University of Manchester, Manchester, M1 7ND, UK John Fletcher manchester ac uk
    Anal Chem 79:2199-206. 2007
    ..g., m/z 255) in the negative ion mode. To our knowledge, this is the first demonstration of the 3D biomolecular imaging within an actual biological system using TOF-SIMS...
  2. ncbi request reprint TOF-SIMS analysis using C60. Effect of impact energy on yield and damage
    John S Fletcher
    Surface Analysis Research Centre, School of Chemical Engineering and Analytical Science, The University of Manchester, Manchester M60 1QD, UK
    Anal Chem 78:1827-31. 2006
    ..Depth profiling of organic samples demonstrates that when using C60, there does not appear to be any increase in damage evident in the mass spectra as the impact energy is increased...
  3. pmc Label free biochemical 2D and 3D imaging using secondary ion mass spectrometry
    John S Fletcher
    School of Chemical Engineering and Analytical Science, Manchester Interdisciplinary Biocentre, University of Manchester, Manchester M1 7DN, UK
    Curr Opin Chem Biol 15:733-40. 2011
    ....
  4. doi request reprint Three-dimensional mass spectral imaging of HeLa-M cells--sample preparation, data interpretation and visualisation
    John S Fletcher
    Manchester Interdisciplinary Biocentre, School of Chemical Engineering and Analytical Science, University of Manchester, 131 Princess Street, Manchester M1 7DN, UK
    Rapid Commun Mass Spectrom 25:925-32. 2011
    ..Insights into data interpretation are included and we present methods for 3D reconstruction of the data using multivariate analysis techniques...
  5. doi request reprint A new dynamic in mass spectral imaging of single biological cells
    John S Fletcher
    Manchester Interdisciplinary Biocentre, School of Chemical Engineering and Analytical Science, University of Manchester, Manchester, UK
    Anal Chem 80:9058-64. 2008
    ..We also demonstrate large area mapping and the MS/MS capability of the instrument...
  6. ncbi request reprint Cellular imaging with secondary ion mass spectrometry
    John S Fletcher
    Manchester Interdisciplinary Biocentre, University of Manchester, Manchester, UK
    Analyst 134:2204-15. 2009
    ..Additionally, sample preparation and handling, and data manipulation and visualisation in SIMS are discussed...
  7. ncbi request reprint Explanatory multivariate analysis of ToF-SIMS spectra for the discrimination of bacterial isolates
    Seetharaman Vaidyanathan
    School of Chemical Engineering and Analytical Science, Manchester Interdisciplinary Biocentre, University of Manchester, 131 Princess Street, Manchester, UK M1 7ND
    Analyst 134:2352-60. 2009
    ..A database search of the identified signals enabled the biochemical origins of some these signals to be postulated...
  8. doi request reprint Effects of cryogenic sample analysis on molecular depth profiles with TOF-secondary ion mass spectrometry
    Alan M Piwowar
    Surface Analysis Research Centre, Manchester Interdisciplinary Biocentre, School of Chemical Engineering and Analytical Science, The University of Manchester, Manchester M13 9PL, United Kingdom
    Anal Chem 82:8291-9. 2010
    ..0 ± 0.4 × 10(-14) cm(2) at 300 K to 2.0 ± 0.3 × 10(-14) cm(2) at 80 K. The role of water as the facilitator for this reduction is explored through the use of H(2)O and D(2)O dosing experiments at 80 K...
  9. doi request reprint TOF-SIMS with argon gas cluster ion beams: a comparison with C60+
    Sadia Rabbani
    Manchester Interdisciplinary Biocentre, School of Chemical Engineering and Analytical Science, University of Manchester, 131 Princess Street, Manchester, M13 9PL, UK
    Anal Chem 83:3793-800. 2011
    ..However, there also appears to be a reduction in the ionization of secondary species as the size of the Ar cluster increases...
  10. doi request reprint Subsurface biomolecular imaging of Streptomyces coelicolor using secondary ion mass spectrometry
    Seetharaman Vaidyanathan
    School of Chemical Engineering and Analytical Sciences, and School of Chemistry, Manchester Interdisciplinary Biocentre, The University of Manchester, 131 Princess Street, Manchester M1 7DN, UK
    Anal Chem 80:1942-51. 2008
    ....
  11. ncbi request reprint Properties of C84 and C24H12 molecular ion sources for routine TOF-SIMS analysis
    Gregory X Biddulph
    Manchester Interdisciplinary Biocentre, University of Manchester, Manchester, M1 7DN, UK
    Anal Chem 79:7259-66. 2007
    ..The C60 was found to ameliorate this effect, possibly due to the increase in protonation in polyatomic sputtering, and coronene was found to further reduce suppression showing evidence of a chemical effect...
  12. pmc Enhancing secondary ion yields in time of flight-secondary ion mass spectrometry using water cluster primary beams
    Sadia Sheraz Née Rabbani
    Manchester Institute of Biotechnology, The University of Manchester, Manchester, United Kingdom
    Anal Chem 85:5654-8. 2013
    ..These results provide encouragement that further developments of the water cluster beam to higher energies and larger cluster sizes will provide the ion yield enhancements necessary for the future development of TOF-SIMS...
  13. doi request reprint A new SIMS paradigm for 2D and 3D molecular imaging of bio-systems
    John S Fletcher
    Manchester Interdisciplinary Biocentre, School of Chemical Engineering and Analytical Science, The University of Manchester, Manchester, M1 7DN, UK
    Anal Bioanal Chem 396:85-104. 2010
    ..Early results from these instruments suggest that sub-micron analysis and imaging with high mass resolution and good ion yields are now realizable, although the low ion yield issue still needs to be solved...
  14. doi request reprint Developments in molecular SIMS depth profiling and 3D imaging of biological systems using polyatomic primary ions
    John S Fletcher
    Manchester Interdisciplinary Biocentre, School of Chemical Engineering and Analytical Science, University of Manchester, Manchester M60 1QD, UK
    Mass Spectrom Rev 30:142-74. 2011
    ..However, although prospects are good, the review highlights the continuing challenges presented by the low ionization efficiency and the complications that arise from matrix effects...
  15. doi request reprint The inherent problem of transflection-mode infrared spectroscopic microscopy and the ramifications for biomedical single point and imaging applications
    Paul Bassan
    Manchester Institute of Biotechnology, University of Manchester, 131 Princess Street, Manchester, M1 7DN, UK
    Analyst 138:144-57. 2013
    ..We show that spectra can be distorted such that classification fails leading to inaccurate tissue segmentation which may have subsequent implications for disease diagnostics applications...