T P Fleming

Summary

Affiliation: University of Southampton
Country: UK

Publications

  1. pmc Mouse embryo culture induces changes in postnatal phenotype including raised systolic blood pressure
    Adam J Watkins
    School of Biological Sciences, University of Southampton, Bassett Crescent East, Southampton SO16 7PX, UK
    Proc Natl Acad Sci U S A 104:5449-54. 2007
  2. pmc Metabolic induction and early responses of mouse blastocyst developmental programming following maternal low protein diet affecting life-long health
    Judith J Eckert
    Faculty of Medicine, University of Southampton, Southampton General Hospital, Southampton, United Kingdom
    PLoS ONE 7:e52791. 2012
  3. ncbi request reprint Nutrition of females during the peri-conceptional period and effects on foetal programming and health of offspring
    T P Fleming
    School of Biological Sciences, University of Southampton, Southampton General Hospital, United Kingdom
    Anim Reprod Sci 130:193-7. 2012
  4. ncbi request reprint Adaptive responses of the embryo to maternal diet and consequences for post-implantation development
    Tom P Fleming
    School of Biological Sciences, University of Southampton, Mailpoint 840, Southampton General Hospital, Tremona Road, Southampton SO16 6YD, UK
    Reprod Fertil Dev 24:35-44. 2011
  5. pmc Mouse maternal systemic inflammation at the zygote stage causes blunted cytokine responsiveness in lipopolysaccharide-challenged adult offspring
    Charlotte L Williams
    School of Biological Sciences, University of Southampton, Mailpoint 840, Level D Laboratories and Pathology Block, Southampton General Hospital, Tremona Road, Southampton SO16 6YD, UK
    BMC Biol 9:49. 2011
  6. ncbi request reprint The embryo and its future
    Tom P Fleming
    School of Biological Sciences, University of Southampton, Southampton SO16 7PX, United Kingdom
    Biol Reprod 71:1046-54. 2004
  7. ncbi request reprint Society for Reproductive Biology Founders' Lecture 2003. The making of an embryo: short-term goals and long-term implications
    Tom P Fleming
    Division of Cell Sciences, School of Biological Sciences, University of Southampton, Bassett Crescent East, Southampton, UK
    Reprod Fertil Dev 16:325-37. 2004
  8. ncbi request reprint Assembly of tight junctions during early vertebrate development
    T P Fleming
    Division of Cell Sciences, School of Biological Sciences, University of Southampton, Bassett Crescent East, Southampton SO16 7PX, UK
    Semin Cell Dev Biol 11:291-9. 2000
  9. ncbi request reprint Cell adhesion in the preimplantation mammalian embryo and its role in trophectoderm differentiation and blastocyst morphogenesis
    T P Fleming
    Division of Cell Sciences, School of Biological Sciences, University of Southampton, Basett Crescent East, Southampton SO16 7PX, UK
    Front Biosci 6:D1000-7. 2001
  10. ncbi request reprint Tight junction assembly during mouse blastocyst formation is regulated by late expression of ZO-1 alpha+ isoform
    B Sheth
    Division of Cell Sciences, School of Biological Sciences, University of Southampton, UK
    Development 124:2027-37. 1997

Research Grants

Collaborators

Detail Information

Publications42

  1. pmc Mouse embryo culture induces changes in postnatal phenotype including raised systolic blood pressure
    Adam J Watkins
    School of Biological Sciences, University of Southampton, Bassett Crescent East, Southampton SO16 7PX, UK
    Proc Natl Acad Sci U S A 104:5449-54. 2007
    ..These animal data indicate that postnatal physiological criteria important in cardiovascular and metabolic health may be more sensitive to routine ART procedures than growth...
  2. pmc Metabolic induction and early responses of mouse blastocyst developmental programming following maternal low protein diet affecting life-long health
    Judith J Eckert
    Faculty of Medicine, University of Southampton, Southampton General Hospital, Southampton, United Kingdom
    PLoS ONE 7:e52791. 2012
    ..Moreover, this induction step associates with changes in extra-embryonic trophectoderm behaviour occurring as early compensatory responses leading to later nutrient recovery...
  3. ncbi request reprint Nutrition of females during the peri-conceptional period and effects on foetal programming and health of offspring
    T P Fleming
    School of Biological Sciences, University of Southampton, Southampton General Hospital, United Kingdom
    Anim Reprod Sci 130:193-7. 2012
    ..The concept that adult disease risk may owe its origin to the quality of peri-conceptional maternal nutrition is one, which merits further research for mechanistic understanding and devising preventive strategies...
  4. ncbi request reprint Adaptive responses of the embryo to maternal diet and consequences for post-implantation development
    Tom P Fleming
    School of Biological Sciences, University of Southampton, Mailpoint 840, Southampton General Hospital, Tremona Road, Southampton SO16 6YD, UK
    Reprod Fertil Dev 24:35-44. 2011
    ..This review assesses the field of PC nutrition in vivo on short- and long-term developmental consequences in rodent and ruminant models and considers the implications for human health...
  5. pmc Mouse maternal systemic inflammation at the zygote stage causes blunted cytokine responsiveness in lipopolysaccharide-challenged adult offspring
    Charlotte L Williams
    School of Biological Sciences, University of Southampton, Mailpoint 840, Level D Laboratories and Pathology Block, Southampton General Hospital, Tremona Road, Southampton SO16 6YD, UK
    BMC Biol 9:49. 2011
    ....
  6. ncbi request reprint The embryo and its future
    Tom P Fleming
    School of Biological Sciences, University of Southampton, Southampton SO16 7PX, United Kingdom
    Biol Reprod 71:1046-54. 2004
    ..We conclude that a complex of different mechanisms may operate to associate early embryo environment with future health...
  7. ncbi request reprint Society for Reproductive Biology Founders' Lecture 2003. The making of an embryo: short-term goals and long-term implications
    Tom P Fleming
    Division of Cell Sciences, School of Biological Sciences, University of Southampton, Bassett Crescent East, Southampton, UK
    Reprod Fertil Dev 16:325-37. 2004
    ..Some current concepts of embryo-environment interactions, which may impact on future health, are also reviewed...
  8. ncbi request reprint Assembly of tight junctions during early vertebrate development
    T P Fleming
    Division of Cell Sciences, School of Biological Sciences, University of Southampton, Bassett Crescent East, Southampton SO16 7PX, UK
    Semin Cell Dev Biol 11:291-9. 2000
    ....
  9. ncbi request reprint Cell adhesion in the preimplantation mammalian embryo and its role in trophectoderm differentiation and blastocyst morphogenesis
    T P Fleming
    Division of Cell Sciences, School of Biological Sciences, University of Southampton, Basett Crescent East, Southampton SO16 7PX, UK
    Front Biosci 6:D1000-7. 2001
    ....
  10. ncbi request reprint Tight junction assembly during mouse blastocyst formation is regulated by late expression of ZO-1 alpha+ isoform
    B Sheth
    Division of Cell Sciences, School of Biological Sciences, University of Southampton, UK
    Development 124:2027-37. 1997
    ....
  11. ncbi request reprint Differentiation of the epithelial apical junctional complex during mouse preimplantation development: a role for rab13 in the early maturation of the tight junction
    B Sheth
    Division of Cell Sciences, School of Biological Sciences, University of Southampton, Bassett Crescent East, SO16 7PX, Southampton, UK
    Mech Dev 97:93-104. 2000
    ..Moreover, we propose a role for rab13 in the specification of the AJC site and the formation and segregation of the TJ...
  12. ncbi request reprint Post-translational control of occludin membrane assembly in mouse trophectoderm: a mechanism to regulate timing of tight junction biogenesis and blastocyst formation
    B Sheth
    Division of Cell Sciences, School of Biological Sciences, University of Southampton, Bassett Crescent East, Southampton SO16 7PX, UK
    Development 127:831-40. 2000
    ....
  13. ncbi request reprint Tight junction protein cingulin is expressed by maternal and embryonic genomes during early mouse development
    Q Javed
    Department of Biology, University of Southampton, UK
    Development 117:1145-51. 1993
    ..Taken together, the results suggest that (i) cingulin may have a role during oogenesis and (ii) cell-cell contact patterns regulate cingulin biosynthesis during early morphogenesis, contributing to lineage-specific epithelial maturation...
  14. ncbi request reprint Tight junction protein ZO-2 expression and relative function of ZO-1 and ZO-2 during mouse blastocyst formation
    Bhavwanti Sheth
    School of Biological Sciences, University of Southampton, Bassett Crescent East, Southampton SO167PX, UK
    Exp Cell Res 314:3356-68. 2008
    ..In contrast, ZO-1 knockdown, or combined ZO-1 and ZO-2 knockdown, generated a more severe inhibition of blastocoel formation indicating distinct roles for ZO proteins in blastocyst morphogenesis...
  15. ncbi request reprint Cloning, sequence analysis and expression pattern of mouse desmocollin 2 (DSC2), a cadherin-like adhesion molecule
    J E Lorimer
    Cancer Research Campaign Epithelial Morphogenesis Research Group, School of Biological Sciences, University of Manchester, UK
    Mol Membr Biol 11:229-36. 1994
    ..In situ hybridization shows that the gene is most strongly expressed suprabasally in stratified epithelia, similar to the expression of bovine DSC2...
  16. ncbi request reprint Gene expression regulating epithelial intercellular junction biogenesis during human blastocyst development in vitro
    M Reza Ghassemifar
    Division of Cell Sciences, School of Biological Sciences, University of Southampton, Bassett Crescent East, Southampton SO16 7PX, UK
    Mol Hum Reprod 9:245-52. 2003
    ..Immunoconfocal microscopy analysis of selected adhesion and TJ proteins in human embryos indicated poor membrane assembly compared with mouse blastocysts, which may further affect embryo viability...
  17. doi request reprint Tissue-specific selection of reference genes is required for expression studies in the mouse model of maternal protein undernutrition
    E S Lucas
    School of Biological Sciences, University of Southampton, Southampton General Hospital, Southampton, UK
    Theriogenology 76:558-69. 2011
    ..Therefore, we recommend that reference gene selection for gene expression studies should be thoroughly validated for each tissue of interest...
  18. ncbi request reprint Statistical analysis of data in support of the Barker hypothesis, advantages of using random effects regression model in hierarchical data
    C Osmond
    MRC Environmental Epidemiology Unit, University of Southampton, Southampton General Hospital, Southampton SO16 6YD, UK
    Reprod Biomed Online 10:152-3. 2005
    ..Hierarchical data merits multilevel analysis using a 'random effects' model to enable estimation of variances at different levels and easy assessment of other parameters in a complex data structure...
  19. ncbi request reprint Human embryos developing in vitro are susceptible to impaired epithelial junction biogenesis correlating with abnormal metabolic activity
    Judith J Eckert
    School of Biological Sciences, University of Southampton, Bassett Crescent East, Southampton, UK
    Hum Reprod 22:2214-24. 2007
    ..Whether morphological and metabolic criteria can be linked to poor TE differentiation thereby underpinning developmental predictions mechanistically remains unknown...
  20. pmc Low protein diet fed exclusively during mouse oocyte maturation leads to behavioural and cardiovascular abnormalities in offspring
    Adam J Watkins
    School of Biological Sciences, University of Southampton, Bassett Crescent East, Southampton SO16 7PX, UK
    J Physiol 586:2231-44. 2008
    ..Similar and distinct postnatal outcomes were observed here compared with maternal LPD treatment during post-fertilization preimplantation development which may reflect the relative contribution of the paternal genome...
  21. pmc Blastocyst environment and its influence on offspring cardiovascular health: the heart of the matter
    Adam J Watkins
    School of Biological Sciences, University of Southampton, Bassett Crescent East, Southampton, UK
    J Anat 215:52-9. 2009
    ..Here, we review the relationship between preimplantation embryo environment and postnatal cardiovascular disease risk, and consider biochemical, molecular, genetic and physiological pathways implicated in this association...
  22. ncbi request reprint Maternal low-protein diet during mouse pre-implantation development induces vascular dysfunction and altered renin-angiotensin-system homeostasis in the offspring
    Adam J Watkins
    School of Biological Sciences, University of Southampton, Bassett Crescent East, Southampton, UK
    Br J Nutr 103:1762-70. 2010
    ....
  23. ncbi request reprint Occludin TM4(-): an isoform of the tight junction protein present in primates lacking the fourth transmembrane domain
    M Reza Ghassemifar
    Division of Cell Sciences, School of Biological Sciences, University of Southampton, Bassett Crescent East, Southampton SO16 7PX, UK Department of Biology, University of York, PO Box 373, York YO10 5YW, UK
    J Cell Sci 115:3171-80. 2002
    ..These data suggest that the TM4(-) isoform may be translated at low levels in specific conditions and may contribute to regulation of occludin function...
  24. doi request reprint Tight junction biogenesis during early development
    Judith J Eckert
    School of Biological Sciences, University of Southampton, Bassett Crescent East, Southampton, SO16 7PX, UK
    Biochim Biophys Acta 1778:717-28. 2008
    ....
  25. ncbi request reprint Maternal low protein diet restricted to the preimplantation period induces a gender-specific change on hepatic gene expression in rat fetuses
    Wing Yee Kwong
    School of Biological Sciences, University of Southampton, Bassett Crescent East, Southampton, UK
    Mol Reprod Dev 74:48-56. 2007
    ..The present findings show that the long term potential of the preimplantation embryo is sensitive to maternal LPD such that basal levels of hepatic gene expression in day 20 fetuses are altered in a gender-specific manner...
  26. ncbi request reprint Imprinted gene expression in the rat embryo-fetal axis is altered in response to periconceptional maternal low protein diet
    Wing Yee Kwong
    School of Biological Sciences, University of Southampton, Bassett Crescent East, Southampton SO16 7PX, UK
    Reproduction 132:265-77. 2006
    ....
  27. ncbi request reprint Specific PKC isoforms regulate blastocoel formation during mouse preimplantation development
    Judith J Eckert
    Division of Cell Sciences, School of Biological Sciences, University of Southampton, Southampton SO16 7PX, UK
    Dev Biol 274:384-401. 2004
    ..Our results demonstrate for the first time that distinct PKC isotypes contribute to the regulation of cavitation in preimplantation embryos via target proteins including Na+/K+ ATPase...
  28. ncbi request reprint Contribution of JAM-1 to epithelial differentiation and tight-junction biogenesis in the mouse preimplantation embryo
    Fay C Thomas
    School of Biological Sciences, University of Southampton, Bassett Crescent East, Southampton, SO16 7PX, UK
    J Cell Sci 117:5599-608. 2004
    ....
  29. ncbi request reprint PKC signalling regulates tight junction membrane assembly in the pre-implantation mouse embryo
    Judith J Eckert
    University of Southampton, Division of Cell Sciences, School of Biological Sciences, Bassett Crescent East, Southampton SO16 7PX, UK
    Reproduction 127:653-67. 2004
    ....
  30. pmc The influence of mouse Ped gene expression on postnatal development
    Adam Watkins
    School of Biological Sciences, Developmental and Cell Biology Group, University of Southampton, Bassett Crescent East, Southampton SO16 7PX, UK
    J Physiol 571:211-20. 2006
    ..K1 females having a higher ratio for lungs than B6. K2 females (P = 0.014). These data provide evidence of an association between the rate of preimplantation embryo development, postnatal growth and later cardiovascular function...
  31. ncbi request reprint Relative contribution of cell contact pattern, specific PKC isoforms and gap junctional communication in tight junction assembly in the mouse early embryo
    Judith J Eckert
    University of Southampton, School of Biological Sciences, Bassett Crescent East, Southampton, SO16 7PX, UK
    Dev Biol 288:234-47. 2005
    ..Our data suggest that the dynamics of cell contact patterns coordinate the spatial organization of TJ formation via specific PKC signaling pathways during blastocyst biogenesis...
  32. ncbi request reprint The preimplantation embryo: handle with care
    Adam J Watkins
    School of Biological Sciences, University of Southampton, Bassett Crescent East, Southampton, United Kingdom
    Semin Reprod Med 26:175-85. 2008
    ..We consider how fetal and postnatal phenotype may become susceptible to the plasticity of the preimplantation embryo and the risks for adult health and physiology...
  33. ncbi request reprint Adaptive responses by mouse early embryos to maternal diet protect fetal growth but predispose to adult onset disease
    Adam J Watkins
    School of Biological Sciences, University of Southampton, Southampton SO16 7PX, United Kingdom
    Biol Reprod 78:299-306. 2008
    ..However, activation of such responses may also lead to adult excess growth and cardiovascular and behavioral diseases...
  34. ncbi request reprint Tight junction messenger RNA expression levels in bovine embryos are dependent upon the ability to compact and in vitro culture methods
    Daniel J Miller
    Division of Cell Sciences, School of Biological Sciences, University of Southampton, Southampton SO16 7PX, United Kingdom
    Biol Reprod 68:1394-402. 2003
    ..We conclude that TJ mRNA expression levels are sensitive to environmental conditions that may influence the developmental potential of bovine blastocysts...
  35. doi request reprint 93 exposure time is critical in blastocysts adaptations to branch-chain amino-Acid depletion
    M A Velazquez
    University of Southampton, Southampton, United Kingdom
    Reprod Fertil Dev 25:194. 2012
    ..These adaptations depend on exposure time and duration of BCAA depletion, thus potentially contributing to triggering specific developmental consequences...
  36. pmc The alpha isoform of protein kinase C is involved in signaling the response of desmosomes to wounding in cultured epithelial cells
    S Wallis
    School of Biological Sciences, University of Manchester, Manchester, M13 9PT, United Kingdom
    Mol Biol Cell 11:1077-92. 2000
    ..These results show that PKCalpha participates in a novel signaling pathway that modulates desmosomal adhesion in response to wounding...
  37. ncbi request reprint Localisation of tight junction protein cingulin is temporally and spatially regulated during early mouse development
    T P Fleming
    Department of Biology, University of Southampton, UK
    Development 117:1135-44. 1993
    ..Collectively, our results indicate that (i) cingulin from nonjunctional sites does not contribute to tight junction assembly and (ii) the molecular maturation of the junction appears to occur progressively over at least two cell cycles...
  38. pmc Identification of a tight junction-associated guanine nucleotide exchange factor that activates Rho and regulates paracellular permeability
    Gaelle Benais-Pont
    Department of Cell Biology, University of Geneva, 1211 Geneva, Switzerland
    J Cell Biol 160:729-40. 2003
    ..These data indicate that GEF-H1/Lfc is a component of TJs and functions in the regulation of epithelial permeability...
  39. ncbi request reprint Expression of Wnt genes during mouse preimplantation development
    Susan Lloyd
    School of Medicine, Mailpoint 813, Southampton General Hospital, SO16 6YD Southampton, UK
    Gene Expr Patterns 3:309-12. 2003
    ..To our knowledge, expression of Wnt genes has not been previously described at such an early stage of mammalian development...
  40. ncbi request reprint Regulation of desmocollin transcription in mouse preimplantation embryos
    J E Collins
    Department of Biology, School of Biological Sciences, University of Southampton, UK
    Development 121:743-53. 1995
    ..Taken together, these results suggest that the presence of a contact-free cell surface activates DSC2 transcription in the mouse early embryo...
  41. ncbi request reprint Support for Barker hypothesis upheld in rat model of maternal undernutrition during the preimplantation period: application of integrated 'random effects' statistical model
    W Y Kwong
    School of Biological Sciences, University of Southampton, Bassett Crescent East, Southampton SO16 7PX, UK
    Reprod Biomed Online 8:574-6. 2004
    ....
  42. ncbi request reprint Appetite regulatory mechanisms and food intake in mice are sensitive to mismatch in diets between pregnancy and postnatal periods
    Dyan Sellayah
    Institute of Developmental Sciences, Developmental Origins of Health and Disease Division, University of Southampton School of Medicine, Southampton General Hospital, Southampton, UK
    Brain Res 1237:146-52. 2008
    ....

Research Grants1

  1. From embryo to adult: mechanisms of fetal programming
    Tom Fleming; Fiscal Year: 2006
    ..Our multidisciplinary approach has been designed to provide the first integrative assessment of dietary programming mechanisms, from embryo to adult. ..