Mark C Field

Summary

Affiliation: University of Cambridge
Country: UK

Publications

  1. doi request reprint First and last ancestors: reconstructing evolution of the endomembrane system with ESCRTs, vesicle coat proteins, and nuclear pore complexes
    Mark C Field
    University of Cambridge, Tennis Court Road, Cambridge, CB2 1QP, UK
    Curr Opin Cell Biol 21:4-13. 2009
  2. pmc An automated graphics tool for comparative genomics: the Coulson plot generator
    Helen I Field
    LGC Genomics Ltd, Pindar Road, Hoddesdon, Hertfordshire EN11 0WZ, UK
    BMC Bioinformatics 14:141. 2013
  3. pmc Telomeres, tethers and trypanosomes
    Mark C Field
    Department of Pathology University of Cambridge, Cambridge, UK
    Nucleus 3:478-86. 2012
  4. doi request reprint Proteomics on the rims: insights into the biology of the nuclear envelope and flagellar pocket of trypanosomes
    Mark C Field
    Department of Pathology, University of Cambridge, Cambridge, UK
    Parasitology 139:1158-67. 2012
  5. doi request reprint Drug screening by crossing membranes: a novel approach to identification of trypanocides
    Mark C Field
    Department of Pathology, University of Cambridge, Tennis Court Road, Cambridge CB2 1QT, UK
    Biochem J 419:e1-3. 2009
  6. doi request reprint The trypanosome flagellar pocket
    Mark C Field
    Department of Pathology, University of Cambridge, Tennis Court Road, Cambridge, UK
    Nat Rev Microbiol 7:775-86. 2009
  7. ncbi request reprint Signalling the genome: the Ras-like small GTPase family of trypanosomatids
    Mark C Field
    The Molteno Building, Department of Pathology, University of Cambridge, Tennis Court Road, Cambridge, UK, CB2 1QP
    Trends Parasitol 21:447-50. 2005
  8. pmc Chaperone requirements for biosynthesis of the trypanosome variant surface glycoprotein
    Mark C Field
    Department of Pathology, University of Cambridge, Cambridge, United Kingdom
    PLoS ONE 5:e8468. 2010
  9. pmc Rab23 is a flagellar protein in Trypanosoma brucei
    Jennifer H Lumb
    Cambridge Institute for Medical Research, MRC Wellcome Trust Building, Addenbrooke s Hospital, Hills Road, Cambridge, CB2 0XY, UK
    BMC Res Notes 4:190. 2011
  10. ncbi request reprint Intracellular trafficking in the trypanosomatids
    Mark C Field
    Department of Pathology, University of Cambridge, Tennis Court Road, Cambridge, CB2 1QP, UK
    Traffic 8:629-39. 2007

Collaborators

Detail Information

Publications83

  1. doi request reprint First and last ancestors: reconstructing evolution of the endomembrane system with ESCRTs, vesicle coat proteins, and nuclear pore complexes
    Mark C Field
    University of Cambridge, Tennis Court Road, Cambridge, CB2 1QP, UK
    Curr Opin Cell Biol 21:4-13. 2009
    ..These studies, plus the realization of a role for the ESCRT complex as an alternate, but equally ancient, system for membrane deformation are providing insight into the earliest stages of endomembrane evolution...
  2. pmc An automated graphics tool for comparative genomics: the Coulson plot generator
    Helen I Field
    LGC Genomics Ltd, Pindar Road, Hoddesdon, Hertfordshire EN11 0WZ, UK
    BMC Bioinformatics 14:141. 2013
    ..However, representation of such information in a standard spreadsheet is a poor visual means from which to extract patterns within a dataset...
  3. pmc Telomeres, tethers and trypanosomes
    Mark C Field
    Department of Pathology University of Cambridge, Cambridge, UK
    Nucleus 3:478-86. 2012
    ..We discuss these and other recent findings on the organization of nuclei in diverse organisms, and the implications of these findings for the evolutionary origin of eukaryotes...
  4. doi request reprint Proteomics on the rims: insights into the biology of the nuclear envelope and flagellar pocket of trypanosomes
    Mark C Field
    Department of Pathology, University of Cambridge, Cambridge, UK
    Parasitology 139:1158-67. 2012
    ....
  5. doi request reprint Drug screening by crossing membranes: a novel approach to identification of trypanocides
    Mark C Field
    Department of Pathology, University of Cambridge, Tennis Court Road, Cambridge CB2 1QT, UK
    Biochem J 419:e1-3. 2009
    ..These observations may present interesting new leads in the fight against trypanosomiasis, and potentially identify a new target that can be explored for therapeutic potential...
  6. doi request reprint The trypanosome flagellar pocket
    Mark C Field
    Department of Pathology, University of Cambridge, Tennis Court Road, Cambridge, UK
    Nat Rev Microbiol 7:775-86. 2009
    ..In this Review we discuss the contribution of the flagellar pocket to protein trafficking, immune evasion and other processes...
  7. ncbi request reprint Signalling the genome: the Ras-like small GTPase family of trypanosomatids
    Mark C Field
    The Molteno Building, Department of Pathology, University of Cambridge, Tennis Court Road, Cambridge, UK, CB2 1QP
    Trends Parasitol 21:447-50. 2005
    ..brucei with T. cruzi and L. major indicate a high degree of conservation among the species. These analyses provide a framework for the functional investigation of small-GTPase-mediated signalling processes in trypanosomes...
  8. pmc Chaperone requirements for biosynthesis of the trypanosome variant surface glycoprotein
    Mark C Field
    Department of Pathology, University of Cambridge, Cambridge, United Kingdom
    PLoS ONE 5:e8468. 2010
    ..As the variant surface glycoprotein (VSG) represents approximately 90% of trypanosome plasma membrane protein, it is possible that VSG has evolved to fold efficiently to minimize ER folding burden...
  9. pmc Rab23 is a flagellar protein in Trypanosoma brucei
    Jennifer H Lumb
    Cambridge Institute for Medical Research, MRC Wellcome Trust Building, Addenbrooke s Hospital, Hills Road, Cambridge, CB2 0XY, UK
    BMC Res Notes 4:190. 2011
    ..These data may suggest the presence of a Rab23-mediated signaling mechanism in trypanosomes...
  10. ncbi request reprint Intracellular trafficking in the trypanosomatids
    Mark C Field
    Department of Pathology, University of Cambridge, Tennis Court Road, Cambridge, CB2 1QP, UK
    Traffic 8:629-39. 2007
    ....
  11. ncbi request reprint Reconstructing the evolution of the endocytic system: insights from genomics and molecular cell biology
    Mark C Field
    The Molteno Building, Department of Pathology, University of Cambridge, Tennis Court Road, Cambridge CB2 1QP, UK
    Adv Exp Med Biol 607:84-96. 2007
  12. pmc Evolution: On a bender--BARs, ESCRTs, COPs, and finally getting your coat
    Mark C Field
    Department of Pathology, University of Cambridge, Cambridge CB2 1QT, England, UK
    J Cell Biol 193:963-72. 2011
    ..These new insights have had a profound impact on our understanding of the evolutionary origins of the internal architecture of the eukaryotic cell...
  13. pmc Developmental variation in Rab11-dependent trafficking in Trypanosoma brucei
    Belinda S Hall
    Department of Pathology, University of Cambridge, Tennis Court Road, Cambridge CB2 1QP, United Kingdom
    Eukaryot Cell 4:971-80. 2005
    ..These data demonstrate that TbRAB11 controls recycling and is essential in both life stages of T. brucei but that its primary role is subject to developmental variation...
  14. pmc Evidence that low endocytic activity is not directly responsible for human serum resistance in the insect form of African trypanosomes
    Senthil Ka Natesan
    Department of Pathology, Tennis Court Road, University of Cambridge, Cambridge, CB2 1QP, UK
    BMC Res Notes 3:63. 2010
    ..Here we asked if endocytic down-regulation in the procyclic form was associated with resistance to innate trypanolytic immune factors in the blood meal or tsetse fly midgut...
  15. doi request reprint How complex is GTPase signaling in trypanosomes?
    Mark C Field
    The Molteno Building, Department of Pathology, University of Cambridge, Tennis Court Road, Cambridge, CB2 1QP, UK
    Trends Parasitol 24:253-7. 2008
    ..Here, we ask how complex are these small GTPase signaling pathways in trypanosomes? We seek to address this issue by comparisons with the representation of both the GTPase molecules and their accessory factors in several genomes...
  16. pmc NUP-1 Is a large coiled-coil nucleoskeletal protein in trypanosomes with lamin-like functions
    Kelly N DuBois
    Department of Pathology, University of Cambridge, Cambridge, United Kingdom
    PLoS Biol 10:e1001287. 2012
    ..Thus, analogous to vertebrate lamins, NUP-1 is a major component of the nucleoskeleton with key roles in organization of the nuclear periphery, heterochromatin, and epigenetic control of developmentally regulated loci...
  17. pmc The trypanosome Rab-related proteins RabX1 and RabX2 play no role in intracellular trafficking but may be involved in fly infectivity
    Senthil Kumar A Natesan
    Department of Pathology, University of Cambridge, Cambridge, United Kingdom
    PLoS ONE 4:e7217. 2009
    ..We wished to determine whether RabX1 and RabX2 are involved in vesicle transport or other cellular processes...
  18. doi request reprint Ubiquitylation is required for degradation of transmembrane surface proteins in trypanosomes
    Wei Lian Chung
    Department of Pathology, University of Cambridge, Cambridge, UK
    Traffic 9:1681-97. 2008
    ..Hence, ubiquitylation is an evolutionarily conserved mechanism for internalization of surface proteins, but aspects of the machinery differ substantially between the major eukaryotic lineages...
  19. doi request reprint The single ENTH-domain protein of trypanosomes; endocytic functions and evolutionary relationship with epsin
    Carme Gabernet-Castello
    Department of Pathology, University of Cambridge, Tennis Court Road, Cambridge, CB2 1QP, UK
    Traffic 10:894-911. 2009
    ..We suggest that recruitment of a UIM to the ENTH-domain proteins was not essential for participation in endocytosis of ubiquitylated molecules, and is presumably a specific innovation restricted to higher eukaryotes...
  20. pmc Trypanosoma brucei: trypanosome-specific endoplasmic reticulum proteins involved in variant surface glycoprotein expression
    Ya nan Wang
    Department of Pathology, University of Cambridge, Tennis Court Road, Cambridge, UK
    Exp Parasitol 125:208-21. 2010
    ..Therefore, ERAP32 and ERAP18 are trypanosome-specific ER-localized proteins with a major role in VSG protein export and, contrary to current paradigms, VSG is not saturated on the cell surface...
  21. pmc Evidence for recycling of invariant surface transmembrane domain proteins in African trypanosomes
    V Lila Koumandou
    Department of Pathology, University of Cambridge, Cambridge, United Kingdom
    Eukaryot Cell 12:330-42. 2013
    ....
  22. pmc The trypanosome transcriptome is remodelled during differentiation but displays limited responsiveness within life stages
    V Lila Koumandou
    The Molteno Building, Department of Pathology, University of Cambridge, Tennis Court Road, Cambridge, CB2 1QP, UK
    BMC Genomics 9:298. 2008
    ....
  23. pmc Ubiquitylation and developmental regulation of invariant surface protein expression in trypanosomes
    Ka Fai Leung
    Department of Pathology, University of Cambridge, Tennis Court Road, Cambridge, United Kingdom
    Eukaryot Cell 10:916-31. 2011
    ..We suggest that ubiquitylation may be a general mechanism for regulating trans-membrane domain surface proteins in trypanosomes...
  24. pmc Evolution of Tre-2/Bub2/Cdc16 (TBC) Rab GTPase-activating proteins
    Carme Gabernet-Castello
    Department of Pathology, University of Cambridge, Cambridge CB2 1QP, United Kingdom
    Mol Biol Cell 24:1574-83. 2013
    ..The reconstruction also shows conservation of ancestral TBC subfamilies, continuing evolution of new TBCs, and frequent secondary losses. These patterns give additional insights into the sculpting of the endomembrane system...
  25. doi request reprint Evolution of the multivesicular body ESCRT machinery; retention across the eukaryotic lineage
    Ka Fai Leung
    Department of Pathology, University of Cambridge, Tennis Court Road, Cambridge, UK
    Traffic 9:1698-716. 2008
    ..Thus the ESCRT system is an ancient and well-conserved feature of eukaryotic cells but with key differences between diverse lineages...
  26. pmc Activation of endocytosis as an adaptation to the mammalian host by trypanosomes
    Senthil Kumar A Natesan
    The Molteno Building, Department of Pathology, Tennis Court Road, University of Cambridge, Cambridge CB2 1QP, United Kingdom
    Eukaryot Cell 6:2029-37. 2007
    ..These data suggest that high-level endocytosis in Trypanosoma brucei is an adaptation required for viability in the mammalian host...
  27. doi request reprint Analysis of small GTPase function in trypanosomes
    Mark C Field
    Department of Pathology, University of Cambridge, Cambridge, United Kingdom
    Methods Enzymol 438:57-76. 2008
    ....
  28. pmc Rab28 function in trypanosomes: interactions with retromer and ESCRT pathways
    Jennifer H Lumb
    Department of Pathology, University of Cambridge, Tennis Court Road, Cambridge CB2 1QP, UK
    J Cell Sci 124:3771-83. 2011
    ..We suggest that Rab28 coordinates the activity of retromer-dependent trafficking and ESCRT-mediated degradative pathways...
  29. pmc Rab11 function in Trypanosoma brucei: identification of conserved and novel interaction partners
    Carme Gabernet-Castello
    Department of Pathology, University of Cambridge, Tennis Court Road, Cambridge CB2 1QP, United Kingdom
    Eukaryot Cell 10:1082-94. 2011
    ..These data suggest that Rab11 functions are mediated by evolutionarily conserved (i.e., AZI1 and Sec15) and potentially lineage-specific (RBP74) interactions essential for the integration of the endomembrane system...
  30. doi request reprint Evolution of specificity in the eukaryotic endomembrane system
    Joel B Dacks
    The Molteno Building, Department of Pathology, University of Cambridge, Tennis Court Road, Cambridge CB2 1QP, UK
    Int J Biochem Cell Biol 41:330-40. 2009
    ....
  31. pmc A cell-surface phylome for African trypanosomes
    Andrew P Jackson
    Pathogen Genomics Group, Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge, England, United Kingdom
    PLoS Negl Trop Dis 7:e2121. 2013
    ..All data can be accessed at: http://www.genedb.org/Page/trypanosoma_surface_phylome...
  32. doi request reprint Proteomic analysis of clathrin interactions in trypanosomes reveals dynamic evolution of endocytosis
    Vincent O Adung'a
    Department of Pathology, University of Cambridge, Tennis Court Road, Cambridge, CB2 1QP, UK
    Traffic 14:440-57. 2013
    ....
  33. pmc A novel Rho-like protein TbRHP is involved in spindle formation and mitosis in trypanosomes
    Kanwal Abbasi
    Department of Pathology, University of Cambridge, Cambridge, United Kingdom
    PLoS ONE 6:e26890. 2011
    ..160.4180) is annotated, contrasting with the >70 Rho GAP proteins from Homo sapiens. We wished to establish the function(s) of TbRHP and if Tb09.160.4180 is a potential GAP for this protein...
  34. pmc Control systems for membrane fusion in the ancestral eukaryote; evolution of tethering complexes and SM proteins
    V Lila Koumandou
    Department of Pathology, University of Cambridge, Tennis Court Road, Cambridge, CB2 1QP, UK
    BMC Evol Biol 7:29. 2007
    ..This pattern is also likely for Rabs and various other components of the membrane trafficking machinery...
  35. pmc Phylogeny of endocytic components yields insight into the process of nonendosymbiotic organelle evolution
    Joel B Dacks
    Department of Pathology, University of Cambridge, Tennis Court Road, Cambridge CB2 1QP, United Kingdom
    Proc Natl Acad Sci U S A 105:588-93. 2008
    ..This finding provides an example of a basic cellular system that was simpler in the LCEA than in many extant eukaryotes and yields insight into nonendosymbiotic organelle evolution...
  36. ncbi request reprint Evolution of the eukaryotic membrane-trafficking system: origin, tempo and mode
    Joel B Dacks
    Department of Pathology, University of Cambridge, Tennis Court Road, Cambridge, CB2 1QP, UK
    J Cell Sci 120:2977-85. 2007
    ..The descendents of this common ancestor have undergone further modification of the trafficking machinery; unicellular simplicity and multicellular complexity are the prevailing trend, but there are some remarkable counter-examples...
  37. pmc Differential localization of the two T. brucei poly(A) binding proteins to the nucleus and RNP granules suggests binding to distinct mRNA pools
    Susanne Kramer
    Department of Biochemistry, University of Cambridge, Cambridge, United Kingdom
    PLoS ONE 8:e54004. 2013
    ..The grouping of translation initiation as either colocalizing with PABP1 or with PABP2 can be used to complement interaction studies to further define the translation initiation complexes in kinetoplastids...
  38. pmc TbFRP, a novel FYVE-domain containing phosphoinositide-binding Ras-like GTPase from trypanosomes
    Vincent O Adung'a
    Department of Pathology, University of Cambridge, Tennis Court Road, Cambridge CB2 1QP, UK
    Exp Parasitol 133:255-64. 2013
    ..Together, these data suggest that TbFRP is an endocytic GTPase with a highly unusual mechanism of action that involves proteolysis of the nascent protein and membrane targeting via PI3P...
  39. pmc An evolutionarily conserved coiled-coil protein implicated in polycystic kidney disease is involved in basal body duplication and flagellar biogenesis in Trypanosoma brucei
    Gareth W Morgan
    Department of Pathology, University of Cambridge, Tennis Court Road, Cambridge, United Kingdom
    Mol Cell Biol 25:3774-83. 2005
    ..These data further underscore the role of aberrant flagellar biogenesis as a disease mechanism...
  40. doi request reprint Specializations in a successful parasite: what makes the bloodstream-form African trypanosome so deadly?
    Catarina Gadelha
    Department of Pathology, University of Cambridge, Cambridge, UK
    Mol Biochem Parasitol 179:51-8. 2011
    ....
  41. pmc Evolutionary reconstruction of the retromer complex and its function in Trypanosoma brucei
    V Lila Koumandou
    Department of Pathology, University of Cambridge, Cambridge CB2 1QT, UK
    J Cell Sci 124:1496-509. 2011
    ....
  42. pmc Evolution of the karyopherin-β family of nucleocytoplasmic transport factors; ancient origins and continued specialization
    Amanda J O'Reilly
    Department of Pathology, University of Cambridge, Tennis Court Road, Cambridge, United Kingdom
    PLoS ONE 6:e19308. 2011
    ....
  43. pmc Trypanosoma brucei brucei: endocytic recycling is important for mouse infectivity
    Senthil Kumar A Natesan
    Department of Pathology, Tennis Court Road, University of Cambridge, Cambridge CB2 1QP, UK
    Exp Parasitol 127:777-83. 2011
    ..These data indicate that trypanosomes likely tolerate the effects of partly decreased clathrin expression and alterations in early endocytosis, but are more sensitive to alterations in the recycling arm of the pathway...
  44. pmc Adaptin evolution in kinetoplastids and emergence of the variant surface glycoprotein coat in African trypanosomatids
    Paul T Manna
    Department of Pathology, University of Cambridge, Tennis Court Road, Cambridge CB2 1QP, UK
    Mol Phylogenet Evol 67:123-8. 2013
    ..brucei and closest relatives. Further, loss of AP2 correlates precisely with the presence of VSG genes, supporting a model whereby these two adaptations may function synergistically in immune evasion...
  45. pmc Rab protein evolution and the history of the eukaryotic endomembrane system
    Andrew Brighouse
    Department of Pathology, University of Cambridge, Tennis Court Road, Cambridge, UK
    Cell Mol Life Sci 67:3449-65. 2010
    ....
  46. doi request reprint Macromolecular trafficking and immune evasion in african trypanosomes
    Mark C Field
    Department of Pathology, University of Cambridge, Cambridge CB2 1QT, United Kingdom
    Int Rev Cell Mol Biol 278:1-67. 2009
    ..Here, we discuss current understanding of intracellular trafficking pathways of trypanosomes, and relate these processes to immune evasion strategies by the parasite and avoidance of immune responses from the host...
  47. ncbi request reprint Cytoplasmic targeting signals in transmembrane invariant surface glycoproteins of trypanosomes
    Wei Lien Chung
    Department of Pathology, University of Cambridge, Cambridge CB2 1QP, UK
    J Biol Chem 279:54887-95. 2004
    ..Taken together, these data demonstrate the presence of a lysine-dependent endocytosis signal in the ISG65 family...
  48. ncbi request reprint Dileucine signal-dependent and AP-1-independent targeting of a lysosomal glycoprotein in Trypanosoma brucei
    Clare L Allen
    The Molteno Building, Department of Pathology, Tennis Court Road, University of Cambridge, Cambridge CB2 1QP, UK
    Mol Biochem Parasitol 156:175-90. 2007
    ..These data suggest that p67 is targeted to the lysosome by dileucine-dependent but AP-1-independent mechanisms...
  49. pmc Cell density-dependent ectopic expression in bloodstream form Trypanosoma brucei
    Moazzam Ali
    Department of Pathology, University of Cambridge, Tennis Court Road, Cambridge CB2 1QP, UK
    Exp Parasitol 134:249-55. 2013
    ....
  50. doi request reprint Implications of the new eukaryotic systematics for parasitologists
    Joel B Dacks
    The Molteno Building, Department of Pathology, University of Cambridge, Tennis Court Road, Cambridge CB2 1QP, UK
    Parasitol Int 57:97-104. 2008
    ..Referring to the best evidence-based models for eukaryotic evolution will allow parasitologists to make more accurate and reliable inferences about pathogens that cause significant morbidity and mortality...
  51. doi request reprint The Ancient Small GTPase Rab21 Functions in Intermediate Endocytic Steps in Trypanosomes
    Moazzam Ali
    Department of Pathology, University of Cambridge, Cambridge, United Kingdom
    Eukaryot Cell 13:304-19. 2014
    ..These data suggest that TbRab21 acts downstream of TbRab5A and functions in intimate connection with the trypanosome ESCRT system. ..
  52. doi request reprint Terminal galactosylation of glycoconjugates in Plasmodium falciparum asexual blood stages and Trypanosoma brucei bloodstream trypomastigotes
    Ranjan Ramasamy
    Department of Pathology, University of Cambridge, Cambridge, UK
    Exp Parasitol 130:314-20. 2012
    ..The findings confirm the presence of α-gal in a spectrum of T. brucei bloodstream trypomastigote glycoproteins and glycolipids and indicate its relative absence from P. falciparum asexual blood stage glycoconjugates...
  53. ncbi request reprint Trypanosoma brucei: TbRAB4 regulates membrane recycling and expression of surface proteins in procyclic forms
    Belinda S Hall
    Department of Biological Sciences, Imperial College, London SW7 2AY, UK
    Exp Parasitol 111:160-71. 2005
    ..The conservation of function of Rab4 throughout eukaryotic evolution demonstrated here indicates that the Rab4-mediated trafficking pathway is an extremely ancient component of the endocytic system...
  54. ncbi request reprint Rab4 is an essential regulator of lysosomal trafficking in trypanosomes
    Belinda S Hall
    Department of Biological Sciences, Imperial College, London SW7 2AY, United Kingdom
    J Biol Chem 279:45047-56. 2004
    ..brucei but not in receptor-mediated endocytosis or recycling. These data have implications for the role of Rab4 in other cell systems...
  55. ncbi request reprint The single dynamin-like protein of Trypanosoma brucei regulates mitochondrial division and is not required for endocytosis
    Gareth W Morgan
    Wellcome Trust Laboratories for Molecular Parasitology, Department of Biological Sciences, Imperial College, Exhibition Road, London SW7 2AY, United Kingdom
    J Biol Chem 279:10692-701. 2004
    ..The evolutionary implications of these findings are discussed...
  56. ncbi request reprint The mechanism of oxidative stress stabilization of the thromboxane receptor in COS-7 cells
    François Valentin
    Department of Biological Sciences, Imperial College, Exhibition Road, London SW7 2AZ, United Kingdom
    J Biol Chem 279:8316-24. 2004
    ..Stabilization of the TXA(2)Rbeta by translocation from a degradative compartment, i.e. the ER, can account for the augmentation of receptor density observed in vivo...
  57. pmc Endocytosis of a glycosylphosphatidylinositol-anchored protein via clathrin-coated vesicles, sorting by default in endosomes, and exocytosis via RAB11-positive carriers
    Christoph G Grünfelder
    Max Planck Institut fur Biologie, Abteilung Membranbiochemie, D 72076 Tubingen, Germany
    Mol Biol Cell 14:2029-40. 2003
    ....
  58. ncbi request reprint Leishmania major: clathrin and adaptin complexes of an intra-cellular parasite
    Paul W Denny
    Department of Biological Sciences, Centre for Molecular Microbiology and Infection, Imperial College, London SW7 2AY, UK
    Exp Parasitol 109:33-7. 2005
    ..Clathrin relocalises in amastigotes relative to promastigotes, consistent with developmental alterations to the morphology of the endo-membrane system...
  59. pmc Rab5 and Rab11 mediate transferrin and anti-variant surface glycoprotein antibody recycling in Trypanosoma brucei
    Arun Pal
    Wellcome Trust Laboratories for Molecular Parasitology, Department of Biological Sciences and Centre for Molecular Microbiology and Infection, Imperial College London, London, SW7 2AY, UK
    Biochem J 374:443-51. 2003
    ..The presence of an efficient mechanism for the removal of IgG bound to the external surface of T. brucei and its subsequent proteolysis within the recycling system suggests a role for this pathway in immune evasion...
  60. ncbi request reprint RNAit: an automated web-based tool for the selection of RNAi targets in Trypanosoma brucei
    Seth Redmond
    Department of Biological Sciences, Centre for Molecular Microbiology and Infection, Imperial College London, Exhibition Road, London SW7 2AY, UK
    Mol Biochem Parasitol 128:115-8. 2003
  61. ncbi request reprint The endocytic apparatus of the kinetoplastida. Part II: machinery and components of the system
    Gareth W Morgan
    Wellcome Trust Laboratories for Molecular Parasitology, Dept of Biological Sciences, Imperial College of Science, Technology and Medicine, London, UK
    Trends Parasitol 18:540-6. 2002
    ..We also consider novel mechanisms for sorting that have been proposed to operate in trypanosomes...
  62. ncbi request reprint The kinetoplastida endocytic apparatus. Part I: a dynamic system for nutrition and evasion of host defences
    Gareth W Morgan
    Wellcome Trust Laboratories for Molecular Parasitology, Dept of Biological Sciences, Imperial College of Science, Technology and Medicine, Exhibition Road, London, UK
    Trends Parasitol 18:491-6. 2002
    ..In Part 1, the endocytic system is considered in context of the surface molecules and their potential roles in virulence...
  63. ncbi request reprint Leishmania RAB7: characterisation of terminal endocytic stages in an intracellular parasite
    Paul W Denny
    Wellcome Trust Laboratory for Molecular Parasitology and Centre for Molecular Microbiology and Infection, Department of Biological Sciences, Imperial College of Science, Technology and Medicine, London SW7 2AZ, UK
    Mol Biochem Parasitol 123:105-13. 2002
    ....
  64. ncbi request reprint TbRAB18, a developmentally regulated Golgi GTPase from Trypanosoma brucei
    Tim R Jeffries
    Department of Biological Sciences and Centre for Molecular Microbiology and Infection, Wellcome Trust Laboratories for Molecular Parasitology, Imperial College of Science, Technology and Medicine, Exhibition Road, London, UK
    Mol Biochem Parasitol 121:63-74. 2002
    ..Our data indicate further complexity in the evolution of small GTPases, and highlight the need for robust functional data prior to assignment of members of complex gene families...
  65. ncbi request reprint Differential endocytic functions of Trypanosoma brucei Rab5 isoforms reveal a glycosylphosphatidylinositol-specific endosomal pathway
    Arun Pal
    Wellcome Trust Laboratories for Molecular Parasitology, Department of Biological Sciences, Imperial College of Science, London SW7 2AY, United Kingdom
    J Biol Chem 277:9529-39. 2002
    ..brucei. A developmentally regulated GPI-specific endosomal pathway in the bloodstream form suggests that specialized transport of GPI-anchored proteins is required for survival in the mammalian host...
  66. pmc Chromosome-wide analysis of gene function by RNA interference in the african trypanosome
    Chandra Subramaniam
    School of Biological Sciences, University of Manchester, Oxford Road, Manchester, United Kingdom
    Eukaryot Cell 5:1539-49. 2006
    ....
  67. ncbi request reprint TbRAB23; a nuclear-associated Rab protein from Trypanosoma brucei
    Vivek Dhir
    Department of Biological Sciences, Imperial College London, London SW7 2AY, UK
    Mol Biochem Parasitol 136:297-301. 2004
  68. ncbi request reprint TbVps34, the trypanosome orthologue of Vps34, is required for Golgi complex segregation
    Belinda S Hall
    Department of Biological Sciences, Imperial College of Science, Technology and Medicine, London SW7 2AY, UK
    J Biol Chem 281:27600-12. 2006
    ..These observations implicate TbVps34 as having a role in coordinating segregation of the Golgi complex at cell division...
  69. ncbi request reprint Identification of a very large Rab GTPase family in the parasitic protozoan Trichomonas vaginalis
    Kalpana Lal
    Department of Zoology, The Natural History Museum, South Kensington, London SW7 5BD, UK
    Mol Biochem Parasitol 143:226-35. 2005
    ..vaginalis both an amoeboid life cycle stage and very large Rab gene families...
  70. ncbi request reprint The genome of the African trypanosome Trypanosoma brucei
    Matthew Berriman
    Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton CB10 1SA, UK
    Science 309:416-22. 2005
    ..brucei and the greatest in L. major. Horizontal transfer of genes of bacterial origin has contributed to some of the metabolic differences in these parasites, and a number of novel potential drug targets have been identified...
  71. ncbi request reprint A bioinformatic analysis of the RAB genes of Trypanosoma brucei
    John P Ackers
    Department of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, Keppel Street, London WC1E 7HT, UK
    Mol Biochem Parasitol 141:89-97. 2005
    ....
  72. ncbi request reprint Perturbation of local endogenous expression by insertion of Pol I expression constructs into the genome of Trypanosoma brucei
    Vivek Dhir
    Department of Biological Sciences, Imperial College, London SW7 2AY, UK
    Exp Parasitol 109:198-200. 2005
    ..These data suggest that local perturbation of the genome by insertion of DNA constructs can have wider impacts on gene expression, which need to be monitored...
  73. ncbi request reprint TbRAB1 and TbRAB2 mediate trafficking through the early secretory pathway of Trypanosoma brucei
    Vivek Dhir
    Department of Biological Sciences, Imperial College, London, SW7 2AY, UK
    Mol Biochem Parasitol 137:253-65. 2004
    ..These data indicate conservation of function for these proteins between trypanosomes and crown eukaryotes...
  74. ncbi request reprint Both of the Rab5 subfamily small GTPases of Trypanosoma brucei are essential and required for endocytosis
    Belinda Hall
    Department of Biological Sciences, Imperial College, Exhibition Road, London SW7 2AY, UK
    Mol Biochem Parasitol 138:67-77. 2004
    ..Taken together, these data indicate that both TbRAB5A and TbRAB5B are required for endocytosis in trypanosomes and demonstrate that there are multiple essential endocytic routes in this organism...
  75. ncbi request reprint Intracellular membrane transport systems in Trypanosoma brucei
    Mark C Field
    Department of Biological Sciences, Imperial College, London, UK
    Traffic 5:905-13. 2004
    ..Recent advances in the study of intracellular transport in T. brucei have been considerable, and a fuller picture of the complexity, function and role that the endomembrane system plays in trypanosomes is finally emerging...
  76. ncbi request reprint Dramatic reorganisation of Trichomonas endomembranes during amoebal transformation: a possible role for G-proteins
    Kalpana Lal
    Department of Zoology, The Natural History Museum, South Kensington, London, UK
    Mol Biochem Parasitol 148:99-102. 2006
  77. ncbi request reprint Evidence for a non-LDL-mediated entry route for the trypanocidal drug suramin in Trypanosoma brucei
    Arun Pal
    Wellcome Trust Laboratories for Molecular Parasitology, Department of Biological Sciences and Centre for Molecular Microbiology and Infection, Imperial College of Science, Technology and Medicine, Exhibition Road, SW7 2AY, London, UK
    Mol Biochem Parasitol 122:217-21. 2002
  78. ncbi request reprint ER-associated protein degradation is a common mechanism underpinning numerous monogenic diseases including Robinow syndrome
    Ying Chen
    Division of Biomedical Sciences, Faculty of Medicine, Imperial College, London SW7 2AZ, UK
    Hum Mol Genet 14:2559-69. 2005
    ..These data both uncover a major pathogenic factor for RRS and indicate that misfolding of secretory proteins is likely to significantly contribute to human disease and morbidity...
  79. ncbi request reprint New approaches to the microscopic imaging of Trypanosoma brucei
    Mark C Field
    Wellcome Trust Laboratories for Molecular Parasitology, Department of Biological Sciences, Imperial College, Exhibition Road, London SW7 2AY, UK
    Microsc Microanal 10:621-36. 2004
    ..brucei at the light and ultrastructural levels, an essential component of analysis of gene function and hence identification of therapeutic targets...
  80. pmc Essential roles for GPI-anchored proteins in African trypanosomes revealed using mutants deficient in GPI8
    Simon Lillico
    Wellcome Centre for Molecular Parasitology, University of Glasgow, The Anderson College, United Kingdom
    Mol Biol Cell 14:1182-94. 2003
    ..These data show that GPI-anchored proteins are essential for the viability of bloodstream form trypanosomes even in the absence of immune challenge and imply that GPI8 is important for proper cell cycle progression...
  81. pmc Clathrin-mediated endocytosis is essential in Trypanosoma brucei
    Clare L Allen
    Wellcome Trust Laboratories for Molecular Parasitology, Department of Biological Sciences, Imperial College, Exhibition Road, London SW7 2AY, UK
    EMBO J 22:4991-5002. 2003
    ..In contrast, a massive internal accumulation of vesicles and significant alterations to trafficking of a lysosomal protein were observed in the procyclic stage, indicating developmental variation in clathrin function in trypanosomes...
  82. ncbi request reprint The role of alternative splicing and C-terminal amino acids in thromboxane receptor stabilization
    François Valentin
    Division of Cell and Molecular Biology, Biochemistry Building, Imperial College, London SW7 2AZ, UK
    Biochem Biophys Res Commun 329:898-904. 2005
    ..These data identify a region of the thromboxane receptor that is responsible for responding to oxidative challenge and open the possibility of identification of the molecular machinery underpinning this response...
  83. ncbi request reprint Reconstitution of glycopeptide export in mixed detergent-solubilised and resealed microsomes depleted of lumenal components
    Bassam R S Ali
    Department of Biological Sciences, Imperial College, London, SW7 2AY, UK
    J Biochem Biophys Methods 62:1-12. 2005
    ..In addition, export competence was highly sensitive to the addition of external protease, indicating a role for protein factors with cytoplasmically exposed determinants...