H J Field

Summary

Affiliation: University of Cambridge
Country: UK

Publications

  1. doi request reprint Substrate specificity and molecular modelling of the feline herpesvirus-1 thymidine kinase
    Islam T M Hussein
    Department of Veterinary Medicine, Cambridge University, Cambridge, UK
    Arch Virol 153:495-505. 2008
  2. doi request reprint Penciclovir is a potent inhibitor of feline herpesvirus-1 with susceptibility determined at the level of virus-encoded thymidine kinase
    Islam T M Hussein
    Department of Veterinary Medicine, University of Cambridge, Madingley Road, Cambridge CB3 0ES, UK
    Antiviral Res 78:268-74. 2008
  3. doi request reprint Development of a quantitative real-time TaqMan PCR assay for testing the susceptibility of feline herpesvirus-1 to antiviral compounds
    Islam T M Hussein
    Department of Veterinary Medicine, University of Cambridge, Madingley Road, Cambridge CB3 0ES, UK
    J Virol Methods 152:85-90. 2008
  4. doi request reprint Recent developments in anti-herpesvirus drugs
    Hugh J Field
    Queens College, Cambridge, UK
    Br Med Bull 106:213-49. 2013
  5. doi request reprint The helicase-primase complex as a target for effective herpesvirus antivirals
    Hugh J Field
    Queens College, Cambridge, UK
    Adv Exp Med Biol 767:145-59. 2013
  6. ncbi request reprint Combinations of antiviral and anti-inflammatory preparations for the topical treatment of herpes simplex virus assessed using a murine zosteriform infection model
    A R Awan
    Centre for Veterinary Science, University of Cambridge, UK
    Antivir Chem Chemother 9:19-24. 1998
  7. ncbi request reprint Herpes simplex virus antiviral drug resistance--current trends and future prospects
    H J Field
    Centre for Veterinary Science, Cambridge University, Madingley Road, CB3 0ES, Cambridge, UK
    J Clin Virol 21:261-9. 2001
  8. doi request reprint Antiviral drug resistance and helicase-primase inhibitors of herpes simplex virus
    Hugh J Field
    Department of Veterinary Medicine, University of Cambridge, Madingley Road, Cambridge CB30ES, United Kingdom
    Drug Resist Updat 14:45-51. 2011
  9. ncbi request reprint Herpesvirus latency and therapy--from a veterinary perspective
    Hugh J Field
    Centre for Veterinary Science, Cambridge University Veterinary School, Madingley Road, Cambridge CB3 0ES, UK
    Antiviral Res 71:127-33. 2006
  10. doi request reprint Effects of therapy using a helicase-primase inhibitor (HPI) in mice infected with deliberate mixtures of wild-type HSV-1 and an HPI-resistant UL5 mutant
    Soumi Sukla
    Department of Veterinary Medicine, University of Cambridge, United Kingdom
    Antiviral Res 87:67-73. 2010

Collaborators

Detail Information

Publications32

  1. doi request reprint Substrate specificity and molecular modelling of the feline herpesvirus-1 thymidine kinase
    Islam T M Hussein
    Department of Veterinary Medicine, Cambridge University, Cambridge, UK
    Arch Virol 153:495-505. 2008
    ..A double substitution of Y29H/F144Y resulted in a threefold increase in the ACV phosphorylation rate...
  2. doi request reprint Penciclovir is a potent inhibitor of feline herpesvirus-1 with susceptibility determined at the level of virus-encoded thymidine kinase
    Islam T M Hussein
    Department of Veterinary Medicine, University of Cambridge, Madingley Road, Cambridge CB3 0ES, UK
    Antiviral Res 78:268-74. 2008
    ..Taken together, these data provided direct evidence that PCV is a potent selective inhibitor of FHV-1 and that the virus-encoded TK is an important determinant of the virus susceptibility to nucleoside analogues...
  3. doi request reprint Development of a quantitative real-time TaqMan PCR assay for testing the susceptibility of feline herpesvirus-1 to antiviral compounds
    Islam T M Hussein
    Department of Veterinary Medicine, University of Cambridge, Madingley Road, Cambridge CB3 0ES, UK
    J Virol Methods 152:85-90. 2008
    ..Therefore, it is suitable for routine anti-FHV-1 drug susceptibility testing in veterinary clinics...
  4. doi request reprint Recent developments in anti-herpesvirus drugs
    Hugh J Field
    Queens College, Cambridge, UK
    Br Med Bull 106:213-49. 2013
    ....
  5. doi request reprint The helicase-primase complex as a target for effective herpesvirus antivirals
    Hugh J Field
    Queens College, Cambridge, UK
    Adv Exp Med Biol 767:145-59. 2013
    ..Finally, herpesvirus latency remains as the most important barrier to a therapeutic cure. Whether or not helicase primase inhibitors alone or in combination with nucleoside analogues can impact on this elusive goal remains to be seen...
  6. ncbi request reprint Combinations of antiviral and anti-inflammatory preparations for the topical treatment of herpes simplex virus assessed using a murine zosteriform infection model
    A R Awan
    Centre for Veterinary Science, University of Cambridge, UK
    Antivir Chem Chemother 9:19-24. 1998
    ..These results are discussed in relation to the inflammation and discomfort experienced by patients and a possible role for anti-inflammatory formulations in the treatment of HSV reactivation episodes in man...
  7. ncbi request reprint Herpes simplex virus antiviral drug resistance--current trends and future prospects
    H J Field
    Centre for Veterinary Science, Cambridge University, Madingley Road, CB3 0ES, Cambridge, UK
    J Clin Virol 21:261-9. 2001
    ..In this review it is argued that the rapid establishment of neuronal latency in the normal pathogenesis of HSV is the key to the low incidence of resistance development and leads to some optimism concerning future trends...
  8. doi request reprint Antiviral drug resistance and helicase-primase inhibitors of herpes simplex virus
    Hugh J Field
    Department of Veterinary Medicine, University of Cambridge, Madingley Road, Cambridge CB30ES, United Kingdom
    Drug Resist Updat 14:45-51. 2011
    ..The possibility of acquired drug-resistance to HPI will then become an issue of great practical importance...
  9. ncbi request reprint Herpesvirus latency and therapy--from a veterinary perspective
    Hugh J Field
    Centre for Veterinary Science, Cambridge University Veterinary School, Madingley Road, Cambridge CB3 0ES, UK
    Antiviral Res 71:127-33. 2006
    ..The review briefly focuses on herpes infections in the horse and cat where some progress has already been achieved in the veterinary antiviral field...
  10. doi request reprint Effects of therapy using a helicase-primase inhibitor (HPI) in mice infected with deliberate mixtures of wild-type HSV-1 and an HPI-resistant UL5 mutant
    Soumi Sukla
    Department of Veterinary Medicine, University of Cambridge, United Kingdom
    Antiviral Res 87:67-73. 2010
    ..All mice inoculated with mixtures remained responsive to BAY 57-1293-therapy with no increase in clinical signs compared to treatment of wt-infected mice...
  11. ncbi request reprint High frequency of spontaneous helicase-primase inhibitor (BAY 57-1293) drug-resistant variants in certain laboratory isolates of HSV-1
    Subhajit Biswas
    Centre for Veterinary Science, Cambridge University Veterinary School, Cambridge, UK
    Antivir Chem Chemother 18:13-23. 2007
    ..Variants resistant to BAY 57-1293 retained sensitivity to the nucleoside analogue, ACV...
  12. ncbi request reprint Detection of HSV-1 variants highly resistant to the helicase-primase inhibitor BAY 57-1293 at high frequency in 2 of 10 recent clinical isolates of HSV-1
    Subhajit Biswas
    Department of Veterinary Medicine, University of Cambridge, and Addenbrooke s Hospital, Cambridge CB3 0ES, UK
    J Antimicrob Chemother 60:274-9. 2007
    ..In contrast, we have shown elsewhere that some laboratory isolates contain resistant variants at higher frequency (10(-4)). Therefore, we screened 10 recent clinical isolates of HSV-1 for BAY 57-1293-resistant virions...
  13. pmc Mismatch primer-based PCR reveals that helicase-primase inhibitor resistance mutations pre-exist in herpes simplex virus type 1 clinical isolates and are not induced during incubation with the inhibitor
    Soumi Sukla
    Department of Veterinary Medicine, University of Cambridge, Madingley Road, Cambridge CB3 0ES, UK
    J Antimicrob Chemother 65:1347-52. 2010
    ..The objective of this study was to provide proof that HPI resistance mutations pre-exist at relatively high frequency in some clinical isolates obtained from individuals naive to HPIs...
  14. ncbi request reprint Single amino acid substitutions in the HSV-1 helicase protein that confer resistance to the helicase-primase inhibitor BAY 57-1293 are associated with increased or decreased virus growth characteristics in tissue culture
    S Biswas
    Centre for Veterinary Science, Cambridge University Veterinary School, Cambridge, U K
    Arch Virol 152:1489-500. 2007
    ..We present evidence that single mutations close to a predicted functional domain of an essential HSV-1 replication enzyme (helicase) are associated with drug resistance and virus growth characteristics...
  15. doi request reprint A single drug-resistance mutation in HSV-1 UL52 primase points to a difference between two helicase-primase inhibitors in their mode of interaction with the antiviral target
    Subhajit Biswas
    Department of Veterinary Medicine, University of Cambridge, Madingley Road, Cambridge CB3 0ES, UK
    J Antimicrob Chemother 61:1044-7. 2008
    ..To investigate the mechanism of action of the helicase-primase inhibitors (HPIs) BAY 57-1293 and BILS 22 BS by selection and characterization of drug-resistant herpes simplex virus (HSV)-1 mutants...
  16. ncbi request reprint The helicase primase inhibitor, BAY 57-1293 shows potent therapeutic antiviral activity superior to famciclovir in BALB/c mice infected with herpes simplex virus type 1
    Subhajit Biswas
    Centre for Veterinary Science, Cambridge University Veterinary School, Madingley Road, Cambridge, CB3 0ES, UK
    Antiviral Res 75:30-5. 2007
    ..Consistent with these findings, BAY 57-1293 also showed a potent antiviral effect in an experiment involving a small number of severely immunocompromised athymic-nude BALB/c mice...
  17. doi request reprint A mutation in helicase motif IV of herpes simplex virus type 1 UL5 that results in reduced growth in vitro and lower virulence in a murine infection model is related to the predicted helicase structure
    Subhajit Biswas
    Department of Veterinary Medicine, University of Cambridge, Cambridge, UK
    J Gen Virol 90:1937-42. 2009
    ..Slower growth and moderately reduced virulence suggest that this mutation might also interfere with the helicase-primase activity...
  18. doi request reprint Mutations close to functional motif IV in HSV-1 UL5 helicase that confer resistance to HSV helicase-primase inhibitors, variously affect virus growth rate and pathogenicity
    Subhajit Biswas
    Department of Veterinary Medicine, University of Cambridge, Cambridge, United Kingdom
    Antiviral Res 80:81-5. 2008
    ..Another BAY 57-1293-resistant UL5 mutant (Lys356Gln), which showed faster growth characteristics in cell culture, however, was at least equally virulent compared to the parent strain...
  19. ncbi request reprint Herpes simplex virus helicase-primase inhibitors: recent findings from the study of drug resistance mutations
    Subhajit Biswas
    Department of Veterinary Medicine, University of Cambridge, Cambridge, UK
    Antivir Chem Chemother 19:1-6. 2008
    ..This article draws attention to the major observations on HPI resistance reported by others and to our own recently published observations that have extended this expanding area of antiviral research...
  20. ncbi request reprint Study of the protective immunity of co-expressed glycoprotein H and L of equine herpesvirus-1 in a murine intranasal infection model
    A Kukreja
    Centre for Veterinary Science, University of Cambridge, UK
    Vet Microbiol 60:1-11. 1998
    ....
  21. ncbi request reprint Temporal pattern of herpes simplex virus type 1 infection and cell death in the mouse brain stem: influence of guanosine nucleoside analogues
    Margaret M Shaw
    University of Cambridge Centre for Veterinary Science, Madingley Road, CB30ES, Cambridge, UK
    J Virol Methods 102:93-102. 2002
    ....
  22. ncbi request reprint Antiviral activity of CTC-8 against herpes simplex virus (HSV-1) in cell culture: evidence for a selective antiviral effect via a cellular mechanism
    Tim Fitzmaurice
    Centre for Veterinary Science, University of Cambridge, Cambridge, UK
    Antivir Chem Chemother 14:217-23. 2003
    ..These results encourage further research into the therapeutic potential of this series of compounds...
  23. ncbi request reprint A serological study of canine herpes virus-1 infection in the English dog population
    M J Reading
    Department of Clinical Veterinary Medicine, Centre for Veterinary Science, Cambridge University
    Arch Virol 143:1477-88. 1998
    ..In contrast with published results from other parts of the world, canine herpes virus-1 infection was shown to be common among the domestic dog population of England...
  24. ncbi request reprint Ganciclovir and penciclovir, but not acyclovir, induce apoptosis in herpes simplex virus thymidine kinase-transformed baby hamster kidney cells
    M M Shaw
    Centre for Veterinary Science, University of Cambridge, Cambridge, UK
    Antivir Chem Chemother 12:175-86. 2001
    ..The current theories regarding apoptosis or necrosis as the preferred form of cell death in prodrug gene therapy are considered and the suitability of PCV or ACV as potential alternatives to GCV in the HSVTK system is discussed...
  25. ncbi request reprint Antiviral Chemistry & Chemotherapy's current antiviral agents FactFile (2nd edition): DNA viruses
    Hugh J Field
    Department of Veterinary Medicine, University of Cambridge, Cambridge, UK
    Antivir Chem Chemother 19:51-62. 2008
    ..Here, we review the current 'state of the art' with old compounds ready to rotate off and new compounds eagerly awaiting to appear on the continuously evolving scene of antiviral drug development...
  26. ncbi request reprint ME-609: a treatment for recurrent herpes simplex virus infections
    Johan G Harmenberg
    Medivir AB, Huddinge, Sweden
    Antivir Chem Chemother 14:205-15. 2003
    ..ME-609 represents a novel treatment principle of recurrent HSV infections and the present paper summarizes the preclinical and early clinical experience of ME-609...
  27. ncbi request reprint Antiviral Chemistry & Chemotherapy's current antiviral agents FactFile (2nd edition): retroviruses and hepadnaviruses
    Erik De Clercq
    Rega Institute for Medical Research, Leuven, Belgium
    Antivir Chem Chemother 19:75-105. 2008
    ..The logic of this new division being the enzymatic similarity between the reverse transcriptase of HIV and hepatitis B virus; the strategies for the development of antiviral agents to combat them have much in common...
  28. ncbi request reprint Antiviral Chemistry & Chemotherapy's current antiviral agents FactFile 2008 (2nd edition): RNA viruses
    Erik De Clercq
    Rega Institute for Medical Research, Leuven, Belgium
    Antivir Chem Chemother 19:63-74. 2008
    ....
  29. ncbi request reprint Antiviral Chemistry & Chemotherapy's current antiviral agents FactFile (2nd edition)
    Hugh J Field
    Antivir Chem Chemother 19:49-50. 2008
  30. pmc Antiviral prodrugs - the development of successful prodrug strategies for antiviral chemotherapy
    Erik De Clercq
    Rega Institute for Medical Research, K U Leuven, Minderbroedersstraat 10, B 3000 Leuven, Belgium
    Br J Pharmacol 147:1-11. 2006
    ..This review will chart the origins and development of the most important of the antiviral prodrugs to date...
  31. ncbi request reprint Antiviral Chemistry & Chemotherapy's current antiviral agents FactFile 2006 (1st edition)
    Hugh J Field
    Antivir Chem Chemother 17:111-2. 2006
  32. ncbi request reprint Antiviral Chemistry & Chemotherapy's current antiviral agents FactFile 2006 (1st edition)
    Erik De Clercq
    Rega Institute for Medical Research, Leuven, Belgium
    Antivir Chem Chemother 17:113-66. 2006
    ..The compounds are grouped by virus targets; thus, the list is sub-divided into inhibitors of DNA viruses, RNA viruses, and retroviruses. The authors welcome comments and suggestions to be incorporated in future editions of the FactFile...