Richard H Ffrench-Constant

Summary

Affiliation: University of Exeter
Country: UK

Publications

  1. ncbi request reprint Which came first: insecticides or resistance?
    Richard H Ffrench-Constant
    Centre for Ecology and Conservation, School of Biosciences, University of Exeter, Cornwall Campus, Tremough, Cornwall TR10 9EZ, UK
    Trends Genet 23:1-4. 2007
  2. ncbi request reprint Insecticidal toxins from Photorhabdus bacteria and their potential use in agriculture
    Richard H Ffrench-Constant
    Center for Ecology and Conservation, School of Biological Sciences, University of Exeter in Cornwall, Penryn, Cornwall TR10 9EZ, UK
    Toxicon 49:436-51. 2007
  3. ncbi request reprint A nematode symbiont sheds light on invertebrate immunity
    Richard H Ffrench-Constant
    School of Biological Sciences, University of Exeter in Cornwall, Penryn, UK
    Trends Parasitol 23:514-7. 2007
  4. ncbi request reprint Evaluating the insecticide resistance potential of eight Drosophila melanogaster cytochrome P450 genes by transgenic over-expression
    Phillip J Daborn
    Centre for Environmental Stress and Adaptation Research CESAR, Department of Genetics, Bio21 Molecular and Biotechnology Institute, The University of Melbourne, VIC 3010, Australia
    Insect Biochem Mol Biol 37:512-9. 2007
  5. ncbi request reprint A Drosophila systems approach to xenobiotic metabolism
    Jingli Yang
    Division of Molecular Genetics, University of Glasgow, Glasgow, United Kingdom
    Physiol Genomics 30:223-31. 2007
  6. pmc Photorhabdus adhesion modification protein (Pam) binds extracellular polysaccharide and alters bacterial attachment
    Robert T Jones
    Department of Biology and Biochemistry, University of Bath, Claverton Down, Bath BA27AY, UK
    BMC Microbiol 10:141. 2010
  7. pmc Pyrosequencing the transcriptome of the greenhouse whitefly, Trialeurodes vaporariorum reveals multiple transcripts encoding insecticide targets and detoxifying enzymes
    Nikos Karatolos
    Rothamsted Research, Harpenden, Hertfordshire, UK
    BMC Genomics 12:56. 2011
  8. pmc Highly conserved gene order and numerous novel repetitive elements in genomic regions linked to wing pattern variation in Heliconius butterflies
    Riccardo Papa
    Department of Biology, University of Puerto Rico Rio Piedras, San Juan, Puerto Rico
    BMC Genomics 9:345. 2008
  9. pmc Next generation transcriptomes for next generation genomes using est2assembly
    Alexie Papanicolaou
    Department of Entomology, Max Planck Institute for Chemical Ecology, Jena, Germany
    BMC Bioinformatics 10:447. 2009
  10. doi request reprint New plasmids and putative virulence factors from the draft genome of an Australian clinical isolate of Photorhabdus asymbiotica
    Paul Wilkinson
    School of Biosciences, University of Exeter in Cornwall, Penryn, UK
    FEMS Microbiol Lett 309:136-43. 2010

Collaborators

  • Mathieu Joron
  • Chris D Jiggins
  • Julian T Dow
  • J Gerrard
  • James Mallet
  • Paul Wilkinson
  • Yannick Pauchet
  • Andrea J Dowling
  • Alexander S Mikheyev
  • Isabella Vlisidou
  • Maria Sanchez-Contreras
  • Ioannis Eleftherianos
  • Richard W Titball
  • Stephen D Bentley
  • A Buckling
  • Nicholas R Waterfield
  • Phillip J Daborn
  • Caroline McCart
  • Melanie R Smee
  • Nikos Karatolos
  • Robert T Jones
  • Alexie Papanicolaou
  • Riccardo Papa
  • Philip Batterham
  • Henry Chung
  • Jingli Yang
  • Guowei Yang
  • Judit Marokhazi
  • Carlos P Silva
  • Michael C Singer
  • David J Hodgson
  • Brian Wee
  • Ian Denholm
  • David R Nelson
  • Martin S Williamson
  • Chris Bass
  • Kevin Gorman
  • Ritika Chauhan
  • A Toby A Jenkins
  • Matthew R Amos
  • Ursula J Potter
  • Todd A Ciche
  • Susan A Joyce
  • Xavier Muñoz-Berbel
  • Abhishek Upadhyay
  • Stefan Bagby
  • Remo Stierli
  • David G Heckel
  • Brian A Counterman
  • Clayton M Morrison
  • Nicola Chamberlain
  • Laura Ferguson
  • James R Walters
  • William O McMillan
  • Rui Chen
  • Robert D Reed
  • Durrell D Kapan
  • Georg Halder
  • Alex Andrianopoulos
  • Michael R Bogwitz
  • Wayn Wong
  • Karen G Greenwood
  • Debra J Woods
  • Adrian Boey
  • Selim Terhzaz
  • Christopher Lumb
  • Brendan W Wren
  • Michelle Hares
  • Edward Feil
  • Andras Fodor
  • Nicholas Waterfield
  • Jason Hinds
  • Richard Stabler
  • Gaelle LeGoff
  • Paul Dean
  • Sadhana Sharma
  • Stuart E Reynolds
  • Candy P Y Au
  • Ursula Potter
  • Timothy Chilver

Detail Information

Publications31

  1. ncbi request reprint Which came first: insecticides or resistance?
    Richard H Ffrench-Constant
    Centre for Ecology and Conservation, School of Biosciences, University of Exeter, Cornwall Campus, Tremough, Cornwall TR10 9EZ, UK
    Trends Genet 23:1-4. 2007
    ..This finding has numerous implications for our understanding of the prevalence of resistance to new compounds. It also indicates that pre-existing resistance alleles might not carry the fitness cost that is associated with new mutations...
  2. ncbi request reprint Insecticidal toxins from Photorhabdus bacteria and their potential use in agriculture
    Richard H Ffrench-Constant
    Center for Ecology and Conservation, School of Biological Sciences, University of Exeter in Cornwall, Penryn, Cornwall TR10 9EZ, UK
    Toxicon 49:436-51. 2007
    ..The likely biological reasons for the massive functional redundancy in Photorhabdus insecticidal toxins are discussed...
  3. ncbi request reprint A nematode symbiont sheds light on invertebrate immunity
    Richard H Ffrench-Constant
    School of Biological Sciences, University of Exeter in Cornwall, Penryn, UK
    Trends Parasitol 23:514-7. 2007
    ..By using RNA interference, it is now also possible to dissect this complex tripartite interaction in a range of both model and non-model hosts...
  4. ncbi request reprint Evaluating the insecticide resistance potential of eight Drosophila melanogaster cytochrome P450 genes by transgenic over-expression
    Phillip J Daborn
    Centre for Environmental Stress and Adaptation Research CESAR, Department of Genetics, Bio21 Molecular and Biotechnology Institute, The University of Melbourne, VIC 3010, Australia
    Insect Biochem Mol Biol 37:512-9. 2007
    ..No increased survival on any insecticide was detected for flies over-expressing either Cyp6a2, Cyp6a8, Cyp6t3, Cyp6a19 or Cyp6a23...
  5. ncbi request reprint A Drosophila systems approach to xenobiotic metabolism
    Jingli Yang
    Division of Molecular Genetics, University of Glasgow, Glasgow, United Kingdom
    Physiol Genomics 30:223-31. 2007
    ..This shows that, although detoxification enzymes are widely distributed, baseline protection against DDT resides primarily in the insect excretory system, corresponding to less than 0.1% of the mass of the organism...
  6. pmc Photorhabdus adhesion modification protein (Pam) binds extracellular polysaccharide and alters bacterial attachment
    Robert T Jones
    Department of Biology and Biochemistry, University of Bath, Claverton Down, Bath BA27AY, UK
    BMC Microbiol 10:141. 2010
    ..We investigated the secreted proteome of a clinical isolate of P. asymbiotica at different temperatures in order to identify proteins relevant to the infection of the two different hosts...
  7. pmc Pyrosequencing the transcriptome of the greenhouse whitefly, Trialeurodes vaporariorum reveals multiple transcripts encoding insecticide targets and detoxifying enzymes
    Nikos Karatolos
    Rothamsted Research, Harpenden, Hertfordshire, UK
    BMC Genomics 12:56. 2011
    ..This 'unigene set' will form a critical reference point for quantitation of over-expressed messages via digital transcriptomics...
  8. pmc Highly conserved gene order and numerous novel repetitive elements in genomic regions linked to wing pattern variation in Heliconius butterflies
    Riccardo Papa
    Department of Biology, University of Puerto Rico Rio Piedras, San Juan, Puerto Rico
    BMC Genomics 9:345. 2008
    ..melpomene. To this end we have undertaken comparative mapping and targeted genomic sequencing in both species. This paper reports on a comparative analysis of genomic sequences linked to color pattern mimicry genes in Heliconius...
  9. pmc Next generation transcriptomes for next generation genomes using est2assembly
    Alexie Papanicolaou
    Department of Entomology, Max Planck Institute for Chemical Ecology, Jena, Germany
    BMC Bioinformatics 10:447. 2009
    ..This rapid growth in data has outstripped the ability of researchers working on non-model species to analyze and mine transcriptome data efficiently...
  10. doi request reprint New plasmids and putative virulence factors from the draft genome of an Australian clinical isolate of Photorhabdus asymbiotica
    Paul Wilkinson
    School of Biosciences, University of Exeter in Cornwall, Penryn, UK
    FEMS Microbiol Lett 309:136-43. 2010
    ..The genome of the Kingscliff strain also contains several genomic differences from the US isolate, whose potential significance in virulence against both humans and insects is discussed...
  11. pmc Diversity of beetle genes encoding novel plant cell wall degrading enzymes
    Yannick Pauchet
    Biosciences, University of Exeter, Penryn, United Kingdom
    PLoS ONE 5:e15635. 2010
    ..Given the huge diversity of beetles alive today, and the diversity of their lifestyles and diets, we predict that beetle guts will emerge as an important new source of enzymes for use in biotechnology...
  12. pmc Genome-wide analysis reveals loci encoding anti-macrophage factors in the human pathogen Burkholderia pseudomallei K96243
    Andrea J Dowling
    Biosciences, University of Exeter, Penryn, United Kingdom
    PLoS ONE 5:e15693. 2010
    ..The detailed analysis of the remaining host of loci will facilitate genetic dissection of the interaction of this important pathogen with host macrophages and thus further elucidate this critical part of its infection cycle...
  13. pmc Microsatellites for the marsh fritillary butterfly: de novo transcriptome sequencing, and a comparison with amplified fragment length polymorphism (AFLP) markers
    Melanie R Smee
    Department of Biology, University of York, York, United Kingdom
    PLoS ONE 8:e54721. 2013
    ....
  14. doi request reprint Pyrosequencing of the midgut transcriptome of the poplar leaf beetle Chrysomela tremulae reveals new gene families in Coleoptera
    Yannick Pauchet
    University of Exeter in Cornwall, Penryn, UK
    Insect Biochem Mol Biol 39:403-13. 2009
    ..We also describe a wealth of new transcripts predicting rapidly evolving gene families involved in plant tissue digestion, which have no homologs in the genome of the stored product pest the Red Flour beetle, Tribolium castaneum...
  15. ncbi request reprint The Mcf1 toxin induces apoptosis via the mitochondrial pathway and apoptosis is attenuated by mutation of the BH3-like domain
    Andrea J Dowling
    Department of Biosciences, University of Exeter, Cornwall Campus, Penryn, Cornwall TR10 9EZ, UK
    Cell Microbiol 9:2470-84. 2007
    ..Together, these results show that Mcf1 mediates apoptosis via the mitochondrial pathway, and are consistent with the hypothesis that the BH3-like domain in Mcf1 is a functional requirement for the pro-apoptotic activity of Mcf1...
  16. pmc Comparative genomics of the emerging human pathogen Photorhabdus asymbiotica with the insect pathogen Photorhabdus luminescens
    Paul Wilkinson
    School of Biosciences, University of Exeter in Cornwall, Penryn TR10 9EZ, UK
    BMC Genomics 10:302. 2009
    ..Here we compare the complete genome of Pa ATCC43949 with that of the previously sequenced insect pathogen P. luminescens strain TT01 which was isolated from its entomopathogenic nematode vector collected from soil in Trinidad and Tobago...
  17. ncbi request reprint Prior infection of Manduca sexta with non-pathogenic Escherichia coli elicits immunity to pathogenic Photorhabdus luminescens: roles of immune-related proteins shown by RNA interference
    Ioannis Eleftherianos
    Department of Biology and Biochemistry, University of Bath, Bath BA2 7AY, UK
    Insect Biochem Mol Biol 36:517-25. 2006
    ..Given the continuous normal exposure of insects to environmental and symbiotic bacteria, we suggest that prior infection is likely to play a significant and underestimated role in determining the level of insect immunity found in nature...
  18. ncbi request reprint The insecticidal toxin makes caterpillars floppy 2 (Mcf2) shows similarity to HrmA, an avirulence protein from a plant pathogen
    Nicholas R Waterfield
    Department of Biology and Biochemistry, University of Bath BA2 7AY, UK
    FEMS Microbiol Lett 229:265-70. 2003
    ..The Mcf1 and Mcf2 toxins therefore belong to a family of high molecular mass toxins, differing at their N-termini, which encode different effector domains...
  19. ncbi request reprint DDT resistance in flies carries no cost
    Caroline McCart
    Curr Biol 15:R587-9. 2005
  20. ncbi request reprint Invertebrates as a source of emerging human pathogens
    Nicholas R Waterfield
    Centre for Molecular Microbiology, Department of Biology and Biochemistry, University of Bath, Bath BA2 7AY, UK
    Nat Rev Microbiol 2:833-41. 2004
    ..This article examines the available evidence for this concept by examining pairs of closely related bacteria, one of which is benign, but insect associated, and one of which is a human pathogen...
  21. ncbi request reprint The genetics and genomics of insecticide resistance
    Richard H Ffrench-Constant
    Department of Biology and Biochemistry, University of Bath, Bath BA2 7AY, UK
    Trends Genet 20:163-70. 2004
    ..These studies enable us to address three fundamental questions in the evolution of resistance. How many genes are involved? How many mutations are there within these genes? How often do these mutations arise in natural populations?..
  22. pmc Using a DNA microarray to investigate the distribution of insect virulence factors in strains of photorhabdus bacteria
    Judit Marokhazi
    Department of Biology and Biochemistry, University of Bath, Bath, BA2 7AY, United Kingdom
    J Bacteriol 185:4648-56. 2003
    ..Using a DNA microarray and comparative genomics, we show that oral toxicity is associated with toxin complex genes tcaABC and that this locus can be mobilized or deleted within different strains...
  23. ncbi request reprint Genomic islands in Photorhabdus
    Nicholas R Waterfield
    Dept of Biology and Biochemistry, University of Bath, South Building, UK
    Trends Microbiol 10:541-5. 2002
    ..Here, we describe novel genomic islands from Photorhabdus that are involved in symbiosis and pathogenicity, and discuss the inter-relationship between virulence factors used against invertebrates and vertebrates...
  24. pmc A conserved supergene locus controls colour pattern diversity in Heliconius butterflies
    Mathieu Joron
    Institute of Evolutionary Biology, School of Biological Sciences, University of Edinburgh, Edinburgh, United Kingdom
    PLoS Biol 4:e303. 2006
    ..We also show that adaptive evolution, both convergent and diversifying, can occur by the repeated involvement of the same genomic regions...
  25. ncbi request reprint RNAi suppression of recognition protein mediated immune responses in the tobacco hornworm Manduca sexta causes increased susceptibility to the insect pathogen Photorhabdus
    Ioannis Eleftherianos
    Department of Biology and Biochemistry, University of Bath, Bath BA2 7AY, UK
    Dev Comp Immunol 30:1099-107. 2006
    ..These results show not only that Photorhabdus is recognized by the Manduca sexta immune system but also that the insect's immune system plays an active, but ultimately ineffective, role in countering infection...
  26. pmc An antibiotic produced by an insect-pathogenic bacterium suppresses host defenses through phenoloxidase inhibition
    Ioannis Eleftherianos
    Department of Biology and Biochemistry, University of Bath, Bath BA2 7AY, United Kingdom
    Proc Natl Acad Sci U S A 104:2419-24. 2007
    ..Thus, ST has a dual function both as a PO inhibitor to counter host immune reactions and also as an antibiotic to exclude microbial competitors from the insect cadaver...
  27. ncbi request reprint Ground control for insect pests
    Richard H Ffrench-Constant
    Nat Biotechnol 24:660-1. 2006
  28. pmc Cis-regulatory elements in the Accord retrotransposon result in tissue-specific expression of the Drosophila melanogaster insecticide resistance gene Cyp6g1
    Henry Chung
    Centre for Environmental and Stress Adaptation Research, Bio21 Molecular Science and Biotechnology Institute, University of Melbourne, Melbourne, Victoria, Australia
    Genetics 175:1071-7. 2007
    ..This study provides a significant example of how changes in tissue-specific gene expression caused by transposable-element insertions can contribute to adaptation...
  29. pmc Nematode symbiont for Photorhabdus asymbiotica
    John G Gerrard
    Gold Coast Hospital, Southport, Queensland, Australia
    Emerg Infect Dis 12:1562-4. 2006
    ..Although the source of infection was previously unknown, we report that the bacterium is found in a symbiotic association with an insect-pathogenic soil nematode of the genus Heterorhabditis...
  30. ncbi request reprint The immunoglobulin family protein Hemolin mediates cellular immune responses to bacteria in the insect Manduca sexta
    Ioannis Eleftherianos
    Department of Biology and Biochemistry, University of Bath, Claverton Down, UK
    Cell Microbiol 9:1137-47. 2007
    ..In contrast, RNAi of PGRP had no effect on any of these cellular immune functions. These results demonstrate the vital role of Hemolin in Manduca cellular immune responses...
  31. ncbi request reprint Bacterial infection of a model insect: Photorhabdus luminescens and Manduca sexta
    Carlos P Silva
    Department of Biology and Biochemistry, University of Bath, Bath BA2 7AY, UK
    Cell Microbiol 4:329-39. 2002
    ..This close association of the bacteria with the gut, and the production of toxins and protease, triggers a massive programmed cell death of the midgut epithelium...