Harminder S Dua

..To investigate the efficacy of alcohol delamination in the management of recurrent corneal erosions (RCEs)...

..To evaluate the effect of gravity on corneal epithelial cell migration in vitro...

..To describe in vivo confocal microscopy (IVCM) findings in patients with limbal stem cell deficiency (LSCD)...

..The authors undertook to re-evaluate the microanatomy of the limbus in order to identify possible sites that may represent a stem cell niche...

..Despite its complexities limbal transplantation does significantly improve vision related quality of life. Autografts give the best results and living related donor grafts are next best. Majority of cadaver grafts fail in 5 years...

..Conjunctivalisation and vascularisation of the corneal surface and persistent or recurring epithelial defects are hallmarks of limbal deficiency...

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..To compare the endothelial protection of sodium hyaluronate and hydroxypropylmethylcellulose against endothelial damage induced by irrigation...

..With the latter option, systemic immunosuppression is required. Amniotic membrane transplantation is a useful adjunct to the above procedures in some instances...

..To evaluate the histologic changes occurring in the transplanted amniotic membrane in human eyes...

..To evaluate the long-term results of limbal stem cell transplantation (LSCT) in patients with ocular surface (OS) disease...

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..We compare the effects of the main denuding procedures, and propose a highly effective standardized alternative...

..To report in vivo confocal microscopic (IVCM) features in corneal edema supported by histopathologic correlation...

..These play an important role in infection and inflammation. In the present study, the authors studied the gene expression levels of hBD3 and hBD9 in healthy subjects and during and after healing of infectious keratitis...

..The purpose of this microarray study was to characterise the transcriptional profile of the LEC and compare with other ocular surface epithelial regions to support our hypothesis that LEC preferentially harbours stem cells (SC)...

..To examine by histopathology the degree of nerve regeneration in human corneal grafts and to determine the anatomic organization and morphology of the regenerated nerves...

..Evidence suggests that pathogen recognition by TLR releases AMPs, altering AMP-TLR profiles in pathological states. This study investigated ocular surface expression of AMP-TLRs in health and disease...

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..The purpose of this study was to further evaluate the distribution, immunophenotypical, and ultra structural characteristics of the LEC as a putative niche of stem cells...

..To describe in vivo confocal microscopy (IVCM) features of the limbus in normal eyes as related to the palisades of Vogt's...

..To study the morphologic characteristics of corneal nerves in patients with advanced keratoconus using the acetylcholinesterase technique in corneal whole mounts...

..Manual dissection is then required to complete the procedure. The aim of the study is to compare these two groups, successful BB versus failed bubble (FB) dissection to determine whether the clinical outcomes were different...

..Herein, the authors' results of amniotic membrane transplantation (AMT) in ocular surface reconstruction against defined outcome measures are reported...

..To investigate the safety and efficacy of alcohol delamination of the corneal epithelium for recalcitrant recurrent corneal erosion syndrome (RCE) in a prospective non-comparative interventional case series...

..This study investigated EGF content in AM in relation to intra- and inter-donor variations and the effect of processing and preservation (handling)...

..To evaluate the early effect of standard and transepithelial collagen cross-linking on human corneal nerves in donor eyes by ex vivo confocal microscopy and acetylcholinesterase staining...

..To report a previously unrecognized benign bilateral widespread conjunctival and corneal proliferative condition with a potential to affect vision...

..To study the long-term changes at donor sites and safety implications for donor eyes used for harvesting tissue for autologous and living-related donor limbal transplants...

..Wetzlar, Germany) and to investigate alteration in corneal density during active and healed stages of bacterial keratitis...

..In the present study, a beta-defensin AMP gene was isolated from the ocular surface for the first time, and its expression was characterized in the presence of ocular inflammation and/or infection...

..To compare the efficacy, predictability, safety, and induced higher-order aberrations (HOAs) between wavefront-guided and non-wavefront-guided ablations...

..However, RNase-7 induction by IL-1β was not affected by inhibiting the NF-κB signalling pathway. In conclusion, our results indicate that RNase-7 expression is specifically mediated via MAPKs but not NF-κB signalling pathways...

..FINANCIAL DISCLOSURE: No author has a financial or proprietary interest in any material or method mentioned...

..To comprehensively study the gross anatomy of human corneal innervation...

..To study the thickness profile of the normal cornea in order to establish any correlation between central and peripheral points...

..Without standardization, there will be inter-membrane variation, which may compromise the desired therapeutic effect of transplant ready amniotic membrane...

..To study the corneal nerves in patients with chronic bullous keratopathy...

..The findings has significant implications both for the understanding of the normal homeostasis of mucosal surfaces and for antimicrobial and anti-inflammatory therapies...

..To determine the distribution of cell membrane proteins and extracellular matrix proteins around the limbal epithelial crypt (LEC) compared with adjacent limbus and corneal epithelium...

..To ascertain in vivo confocal microscopic features of corneal/conjunctival intraepithelial neoplasia (CIN) and correlate these with histology of the same lesions...

..Disagreement between refractive and topographic determination of the astigmatic axis can be considered a prognostic factor for LASIK correction of myopic astigmatism...

..To determine the refractive outcomes of wavefront-optimized laser in situ keratomileusis (LASIK) treatments, in particular to measure corneal densitometry after LASIK using the densitometry function of the Pentacam Scheimpflug system...

..Samples were either cup-loaded onto rehydrated HED-containing strips or rebuffered into HED-containing buffer followed by in-gel rehydration...

..This pilot study seeks to demonstrate activation of human peripheral effector T cells in response to the uveitis candidate autoantigen, retinal S antigen (SAg), using cytokine flow cytometry (CFC)...

..119 to -0.004, p = 0.037). Time in organ culture had little effect on CEPP. CONCLUSIONS: Preselected organ cultured CSL rims from eye banks may offer a viable alternative tissue source for use in allo-limbal transplantation...

..To report a case of malignant glaucoma after diode laser cyclophotocoagulation...

..The positive staining for L-selectin found by immunohistochemistry but not by Western blotting or PCR would indicate the presence of either another ligand from the selectin family or a cross-reactive epitope on corneal keratocytes...

..It leaves behind a very smooth surface, which is ideal for PRK. It also allows for an intact epithelial flap to be lifted as a sheet from the corneal surface and hence is ideally suited for the LASEK technique...

..This method may provide a distinct alternative for the visualisation of vitreous whilst eliminating the pharmacological effects of triamcinolone acetonide suspension...

..Eight patients remain on treatment, and all have clear grafts. CONCLUSIONS: Tacrolimus (FK506) is effective in prevention of rejection in patients with high-risk corneal and limbal grafts...

..These cells play an active part in ocular surface defence and disease. Our aim was to study the migration of immune cells in the human conjunctiva, across the basement membrane and to characterize their phenotypes...

..CONCLUSIONS: Apoptosis is one of the mechanisms that is involved in PVR pathogenesis. Different apoptosis markers suggest different pathways occur in PVR, including Fas/FasL, TRAIL, and TGF-beta2 mediated processes...

..Further studies will undoubtedly reveal the true potential of the membrane, its mechanism(s) of action, and the effective use of this tissue in ophthalmology...

..However, patients should be thoroughly screened for tuberculosis before treatment and followed up closely during and after therapy with infliximab...

..There is no information available to date on the fate of epithelial cells on such explants. This study provides some insight into this and suggests that an expansion of the stem cell pool or its progeny may occur in limbal explants...

..This inadequacy of the classification system probably also explains the difference between outcomes of management of grade IV burns (with AMT) in this series, compared with others...

..The OBF pneumotonometer was found to be most affected by variation in CCT. For all three instruments, the relation between IOP and CCT depended on the corneal pathology and was greatest for FED...

..Major ophthalmic centres should develop and introduce this technique into routine clinical practice. This is best achieved with a team approach including the ophthalmologist, pathologist, microbiologist, and the immunologist...

..To evaluate the results of laser in situ keratomileusis (LASIK) re-treatment for under correction or regression after primary LASIK procedures for myopia and myopic astigmatism...

..CONCLUSION: Erosion of the drainage tube following shunt surgery is a potentially serious problem. It can be successfully managed using a double layer of amniotic membrane...

..The variability in the outcome may reflect the levels of antibodies gaining access to the corneal cells and not the mismatching alone...

..Systemic immunosuppression reduces the risk of rejection in high-risk corneal grafts...

..Extracapsular cataract extraction with a posterior chamber IOL sutured to the posterior surface of the iris and anterior capsule is a useful option in patients with anterior megalophthalmos and cataract...

..The aim of the study was to comprehensively study the gene expression of the ocular AMPs in human corneal limbal epithelial cells stimulated with Acanthamoeba castellanii (AC)...

..Further studies are required to establish the long term efficacy and safety of sirolimus alone or in combination with other steroid sparing immunosuppressants...

..CONCLUSION: This study shows that autologous serum drops can be safely used in an inpatient setting, under a strict protocol of preparation and storage, without significant risk of bacterial contamination and consequent infection...

..The purpose of this review is to describe the most recent and relevant clinical and experimental data about the use of amniotic membrane in ophthalmology...

..The short and long-term outcomes of the various modalities reported in the literature are also described...

..A degree of intermembrane and intramembrane variation is modified by handling. Unless a standardized protocol is adopted that delivers a membrane with consistent constituents, clinical outcomes may vary and comparisons may be invalid...