Research Topics
| D T DrydenSummaryAffiliation: University of Edinburgh Country: UK Publications
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Detail Information
Publications
The in vitro assembly of the EcoKI type I DNA restriction/modification enzyme and its in vivo implicationsD T Dryden
Institute of Cell and Molecular Biology, University of Edinburgh, U K
Biochemistry 36:1065-76. 1997....
The Orf18 gene product from conjugative transposon Tn916 is an ArdA antirestriction protein that inhibits type I DNA restriction-modification systemsDimitra Serfiotis-Mitsa
School of Chemistry, The University of Edinburgh, The King s Buildings, Edinburgh EH9 3JJ, UK
J Mol Biol 383:970-81. 2008..Our results suggest that ArdA can overcome the restriction barrier following conjugation and so helps increase the spread of antibiotic resistance genes by horizontal gene transfer...
Dissection of the DNA mimicry of the bacteriophage T7 Ocr protein using chemical modificationAugoustinos S Stephanou
EaStCHEM School of Chemistry, University of Edinburgh, Edinburgh EH9 3JJ, UK
J Mol Biol 391:565-76. 2009..Our results show that the electrostatic mimicry of Ocr increases the binding affinity for its target enzyme by up to approximately 800-fold...- Fusion of GFP to the M.EcoKI DNA methyltransferase produces a new probe of Type I DNA restriction and modification enzymesKai Chen
School of Chemistry, University of Edinburgh, The King s Buildings, Edinburgh, EH9 3JJ, UK
Biochem Biophys Res Commun 398:254-9. 2010..EcoKI enzyme. Distances determined from the energy transfer experiments corroborate the structural model of M.EcoKI... - DNA translocation by type III restriction enzymes: a comparison of current models of their operation derived from ensemble and single-molecule measurementsDavid T F Dryden
School of Chemistry, The King s Buildings, The University of Edinburgh, Edinburgh, EH9 3JJ, UK
Nucleic Acids Res 39:4525-31. 2011..We also attempt to reconcile the different models and indicate areas for further experimentation to elucidate the mechanism of these enzymes... - DNA mimicry by proteinsD T F Dryden
School of Chemistry, The University of Edinburgh, The King s Buildings, Edinburgh EH9 3JJ, UK
Biochem Soc Trans 34:317-9. 2006..The unusual properties of these protein DNA mimics may provide a foundation for the design of targeted inhibitors of DNA-binding proteins... - DNA mimicry by proteins and the control of enzymatic activity on DNADavid T F Dryden
School of Chemistry, The University of Edinburgh, The King s Buildings, Edinburgh EH9 3JJ, UK
Trends Biotechnol 24:378-82. 2006..This review highlights the roles of DNA mimics in the control of DNA-binding proteins, suggests other possible candidate proteins using DNA mimicry, and puts forward a range of potential uses of DNA mimics... - How much of protein sequence space has been explored by life on Earth?David T F Dryden
School of Chemistry, University of Edinburgh, The King s Buildings, Edinburgh EH9 3JJ, UK
J R Soc Interface 5:953-6. 2008.... - Nucleoside triphosphate-dependent restriction enzymesD T Dryden
Department of Chemistry, University of Edinburgh, Joseph Black Building, The King s Buildings, Mayfield Road, Edinburgh EH9 3JJ, UK
Nucleic Acids Res 29:3728-41. 2001..The only well-documented GTP-dependent restriction enzyme, McrBC, requires methylated target sequences for the initiation of phosphodiester bond cleavage... - Characterisation of the structure of ocr, the gene 0.3 protein of bacteriophage T7C Atanasiu
Institute of Cell and Molecular Biology, The King's Buildings, University of Edinburgh, Edinburgh EH9 3JR, UK
Nucleic Acids Res 29:3059-68. 2001..This charge distribution may be complementary to the charge distribution within the DNA binding site of type I DNA restriction and modification enzymes... - Central modules of the vaccinia virus complement control protein are not in extensive contactM D Kirkitadze
Edinburgh Centre for Protein Technology, Department of Chemistry, University of Edinburgh, West Mains Road, Edinburgh EH9 3JJ, Scotland, U K
Biochem J 344:167-75. 1999.... - Shape of Ocr, the gene 0.3 protein of bacteriophage T7: modeling based on light scattering experimentsJ J Blackstock
Department of Physics and Astronomy, The University of Edinburgh, Edinburgh EH9 3JZ, UK
Biochemistry 40:9944-9. 2001..4 nm by 2.6 nm. The size and shape predicted by this model are consistent with ocr acting as a mimic of the DNA structure bound by type Iota restriction enzymes... - DNA recognition by the methyl-CpG binding domain of MeCP2A Free
Institute of Cell and Molecular Biology, University of Edinburgh, Edinburgh, EH9 3JR, United Kingdom
J Biol Chem 276:3353-60. 2001..The majority of missense mutations within the MBD found in the human genetic disorder Rett syndrome were shown or predicted to affect folding of the domain rather than the DNA recognition event directly... - Tyrosine 27 of the specificity polypeptide of EcoKI can be UV crosslinked to a bromodeoxyuridine-substituted DNA target sequenceA Chen
Institute of Cell and Molecular Biology, University of Edinburgh, UK
Nucleic Acids Res 23:1177-83. 1995..This suggests that tyrosine 27 may make a similar DNA contact in these other enzymes... - Strong physical constraints on sequence-specific target location by proteins on DNA moleculesHenrik Flyvbjerg
School of Chemistry, The King's Buildings, The University of Edinburgh, Edinburgh, EH9 3JJ, UK
Nucleic Acids Res 34:2550-7. 2006..This effectively rules out any significant role in target location for mechanisms invoking one-dimensional, linear diffusion along DNA... - Crystallization and preliminary X-ray analysis of ocr, the product of gene 0.3 of bacteriophage T7S S Sturrock
Institute of Cell and Molecular Biology, University of Edinburgh, Edinburgh, Scotland
Acta Crystallogr D Biol Crystallogr 57:1652-4. 2001..8 A. The presence of caesium was found to be required for good crystal growth. Anomalous X-ray data was used to identify possible positions for Se and Cs atoms in the unit cell... - Alleviation of restriction by DNA condensation and non-specific DNA binding ligandsSteven A Keatch
School of Chemistry, The University of Edinburgh, The King s Buildings, Edinburgh, EH9 3JJ, UK
Nucleic Acids Res 32:5841-50. 2004.... - The structure of the KlcA and ArdB proteins reveals a novel fold and antirestriction activity against Type I DNA restriction systems in vivo but not in vitroDimitra Serfiotis-Mitsa
EaStCHEM School of Chemistry, University of Edinburgh, The King s Buildings, Edinburgh, EH9 3JJ, UK
Nucleic Acids Res 38:1723-37. 2010..We also present the structure determined by NMR spectroscopy of the pBP136 KlcA protein. The structure shows a novel protein fold and it is clearly not a DNA structural mimic... - A mutational analysis of DNA mimicry by ocr, the gene 0.3 antirestriction protein of bacteriophage T7Augoustinos S Stephanou
EaStCHEM School of Chemistry, The University of Edinburgh, The King s Buildings, Edinburgh EH9 3JJ, UK
Biochem Biophys Res Commun 378:129-32. 2009..Contrary to expectations, all of the single and double site mutations of ocr constructed were active as anti-R/M proteins in vivo and in vitro indicating that the mimicry of DNA by ocr is very resistant to change... - The biology of restriction and anti-restrictionMark R Tock
School of Chemistry, The King's Buildings, The University of Edinburgh, Edinburgh, EH9 3JJ, UK
Curr Opin Microbiol 8:466-72. 2005..Recently, there have been several studies that have shed light on the still developing field of restriction-modification and on the newly re-emerging field of anti-restriction... - Time-resolved fluorescence of 2-aminopurine as a probe of base flipping in M.HhaI-DNA complexesRobert K Neely
School of Chemistry, The University of Edinburgh, West Mains Road, Edinburgh EH9 3JJ, UK
Nucleic Acids Res 33:6953-60. 2005..Analysis of the AP fluorescence decay function reveals conformational heterogeneity in the DNA-enzyme complexes that cannot be discerned from the present X-ray structures... - Assembly of EcoKI DNA methyltransferase requires the C-terminal region of the HsdM modification subunitLynn M Powell
Institute of Cell and Molecular Biology, The King's Buildings, University of Edinburgh, Edinburgh, Scotland EH9 3JR, UK
Biophys Chem 103:129-37. 2003..We conclude that the C-terminal region of the modification subunit of EcoKI is essential for the assembly of the EcoKI methyltransferase... - DNA bending by M.EcoKI methyltransferase is coupled to nucleotide flippingTsueu-Ju Su
School of Chemistry The King's Buildings The University of Edinburgh Edinburgh EH9 3JJ, UK
Nucleic Acids Res 33:3235-44. 2005..This energy is not stored in gapped duplexes. In this way, M.EcoKI can determine the methylation status of two adenine bases separated by a considerable distance in double-stranded DNA and select the required enzymatic response... - A nomenclature for restriction enzymes, DNA methyltransferases, homing endonucleases and their genesRichard J Roberts
New England Biolabs, Beverly, MA 01915, USA
Nucleic Acids Res 31:1805-12. 2003..It provides explicit categories for the many different Type II enzymes now identified and provides a system for naming the putative genes found by sequence analysis of microbial genomes... - DNA looping and translocation provide an optimal cleavage mechanism for the type III restriction enzymesNeal Crampton
Department of Pharmacology, University of Cambridge, Tennis Court Road, Cambridge, UK
EMBO J 26:3815-25. 2007..On the basis of these results, we present a model for restriction by type III restriction enzymes and highlight the similarities between this and other classes of restriction enzymes... - Fast-scan atomic force microscopy reveals that the type III restriction enzyme EcoP15I is capable of DNA translocation and loopingNeal Crampton
Department of Pharmacology, University of Cambridge, Tennis Court Road, Cambridge, United Kingdom
Proc Natl Acad Sci U S A 104:12755-60. 2007..On the basis of our results, we conclude that EcoP15I uses two distinct mechanisms to communicate between two recognition sites: diffusive DNA loop formation and ATPase-driven translocation of the intervening DNA contour... - Reeling in the basesDavid T F Dryden
Nat Struct Mol Biol 11:804-6. 2004 - Continuous assays for DNA translocation using fluorescent triplex dissociation: application to type I restriction endonucleasesSarah E McClelland
DNA-Protein Interactions Group, Department of Biochemistry, University of Bristol, Bristol BS8 1TD, UK
J Mol Biol 348:895-915. 2005..Furthermore, we demonstrated that enzymes deficient in DNA cleavage but with maximal ATPase activity showed initiation and translocation rates identical to wild-type, confirming that DNA strand breaks are not a pre-requisite of motion... - Differential stabilization of reaction intermediates: specificity checkpoints for M.EcoRI revealed by transient fluorescence and fluorescence lifetime studiesBen Youngblood
Program in Biomolecular Science and Engineering, University of California, Santa Barbara, CA 93106 9510, USA
Nucleic Acids Res 36:2917-25. 2008..These results provide a plausible explanation of the way in which destabilization of reaction intermediates can lead to changes in substrate specificity...