Affiliation: University of Glasgow
- [Plasmodium protein-kinases and control of cellular proliferation: molecular targets for new antimalarial drugs]Dominique Dorin
Pathol Biol (Paris) 50:223-6. 2002
- Protein kinases as targets for antimalarial intervention: Kinomics, structure-based design, transmission-blockade, and targeting host cell enzymesChristian Doerig
INSERM U609, Wellcome Centre for Molecular Parasitology, University of Glasgow, 120 Glasgow University Place, Glasgow G12 8TA, Scotland, UK
Biochim Biophys Acta 1754:132-50. 2005....
- Plasmodium falciparum liver stage antigen-1 is cross-linked by tissue transglutaminaseWilliam S Nicoll
USMMVP, Malaria Department, NMRC, Silver Spring, MD 20910, USA
Malar J 10:14. 2011..The LSA-1 protein has been described as a flocculent mass, but its role in parasite development has not been determined...
- Antimalarial drug discovery: targeting protein kinasesChristian Doerig
INSERM U609, Wellcome Centre for Molecular Parasitology, Biomedical Research Centre, University of Glasgow, 120 University Place, Glasgow, UK
Expert Opin Ther Targets 11:279-90. 2007..The authors also discuss the possibility of interfering with: i) Plasmodium PKs regulating transmission to the mosquito vector; and ii) host PKs that may be required for parasite survival...
- Protein kinases of malaria parasites: an updateChristian Doerig
INSERM U609, Wellcome Centre for Molecular Parasitology, University of Glasgow Biomedical Research Centre, Glasgow G12 8TA, Scotland, UK
Trends Parasitol 24:570-7. 2008..Here, the authors of most of these communications join to propose an integrated update of the development of the rapidly expanding field of Plasmodium kinomics...
- Protein kinases as targets for anti-parasitic chemotherapyChristian Doerig
Wellcome Centre for Molecular Parasitology, University of Glasgow, INSERM, 56 Dumbarton Road, Glasgow G11 6NU, Scotland, UK
Biochim Biophys Acta 1697:155-68. 2004..The recent resolution of the structure of a Plasmodium protein kinase complexed with small inhibitory molecules opens the way to a rational approach towards the design of anti-parasitic drugs based on kinase inhibition...
- An essential role for the Plasmodium Nek-2 Nima-related protein kinase in the sexual development of malaria parasitesLuc Reininger
INSERM U609 Wellcome Centre for Molecular Parasitology, Biomedical Research Centre, Faculty of Biomedical and Life Sciences, University of Glasgow, 120 University Place, Glasgow G12 8TA, Scotland
J Biol Chem 284:20858-68. 2009..This has implications (i) in our understanding of sexual development of malaria parasites and (ii) in the context of control strategies aimed at interfering with malaria transmission...
- A NIMA-related protein kinase is essential for completion of the sexual cycle of malaria parasitesLuc Reininger
INSERM U609, Wellcome Centre for Molecular Parasitology, University of Glasgow, Glasgow G11 6NU, Scotland, United Kingdom
J Biol Chem 280:31957-64. 2005..Cell cycle progression in the zygote is identified as a likely precondition for its morphological transition to the ookinete and for the successful establishment of a malaria infection in the mosquito...
- Malaria: targeting parasite and host cell kinomesChristian Doerig
INSERM U609 Wellcome Centre for Molecular Parasitology, University of Glasgow, 120 University Place, Glasgow G12 8TA, Scotland, UK
Biochim Biophys Acta 1804:604-12. 2010..We also briefly allude to the possibility of attacking Plasmodium through the inhibition of human PKs that are required for survival of this obligatory intracellular parasite, and which are targets for other human diseases...
- SAM domain-dependent activity of PfTKL3, an essential tyrosine kinase-like kinase of the human malaria parasite Plasmodium falciparumAbdirahman Abdi
INSERM U609, Wellcome Trust Centre for Molecular Parasitology, Glasgow Biomedical Research Centre, 120 University Place, Glasgow, Scotland G12 8TA, UK
Cell Mol Life Sci 67:3355-69. 2010..This study identifies PfTKL3 as a validated drug target amenable to high-throughput screening...
- Plasmodium falciparum regulatory subunit of cAMP-dependent PKA and anion channel conductanceAnais Merckx
INSERM U609, Wellcome Center for Molecular Parasitology, Glasgow Biomedical Research Centre, Glasgow, Scotland, United Kingdom
PLoS Pathog 4:e19. 2008..These findings may also provide an avenue for the development of new intervention strategies targeting this important anion channel and its regulation...
- Disruption of the PfPK7 gene impairs schizogony and sporogony in the human malaria parasite Plasmodium falciparumDominique Dorin-Semblat
INSERM U609, Wellcome Centre for Molecular Parasitology, University of Glasgow, Glasgow G12 8TA, Scotland, United Kingdom
Eukaryot Cell 7:279-85. 2008..Hence, PfPK7 is involved in a pathway that regulates parasite proliferation and development...
- PfeIK1, a eukaryotic initiation factor 2alpha kinase of the human malaria parasite Plasmodium falciparum, regulates stress-response to amino-acid starvationClare Fennell
INSERM U609, Wellcome Centre for Molecular Parasitology, Biomedical Research Centre University of Glasgow, 120 University Place, Glasgow, G12 8TA, UK
Malar J 8:99. 2009....
- PfPK7, an atypical MEK-related protein kinase, reflects the absence of classical three-component MAPK pathways in the human malaria parasite Plasmodium falciparumDominique Dorin
INSERM U609, Wellcome Centre for Molecular Parasitology, Anderson College, 56 Dumbarton Road, Glasgow G11 6NU, Scotland, UK
Mol Microbiol 55:184-96. 2005....
- Functional characterization of both MAP kinases of the human malaria parasite Plasmodium falciparum by reverse geneticsDominique Dorin-Semblat
INSERM U609, Wellcome Centre for Molecular Parasitology, University of Glasgow, Glasgow, Scotland, UK
Mol Microbiol 65:1170-80. 2007..However, Pfmap-2 protein levels are elevated in pfmap-1(-) parasites, suggesting that Pfmap-1 fulfils an important function in asexual parasites that necessitates compensatory adaptation in parasites lacking this enzyme...
- Identification and initial characterization of three novel cyclin-related proteins of the human malaria parasite Plasmodium falciparumAnais Merckx
INSERM U511 team, Wellcome Centre for Molecular Parasitology, Anderson College, 56 Dumbarton Road, Glasgow G11 6NU, Scotland, United Kingdom
J Biol Chem 278:39839-50. 2003..This raises the possibility that "cryptic" cell cycle regulators may be found among the 50% of the open reading frames in the P. falciparum genome that display no homology to any known proteins...
- Protein kinases of the human malaria parasite Plasmodium falciparum: the kinome of a divergent eukaryotePauline Ward
Wellcome Centre for Molecular Parasitology, University of Glasgow, 56 Dumbarton Road, Glasgow G11 6NU, Scotland, UK
BMC Genomics 5:79. 2004..We report an exhaustive analysis of the P. falciparum genomic database (PlasmoDB) aimed at identifying and classifying all ePKs in this organism...
- The cell cycle of parasitic protozoa: potential for chemotherapeutic exploitationTansy C Hammarton
Wellcome Centre for Molecular Parasitology, Anderson College, University of Glasgow, 56 Dumbarton Road, Glasgow, G11 6NU, UK
Prog Cell Cycle Res 5:91-101. 2003..Potential targets include protein kinases from the cyclin-dependent kinase, cAMP-dependent kinase and mitogen activated protein kinase families...
- A comprehensive model of purine uptake by the malaria parasite Plasmodium falciparum: identification of four purine transport activities in intraerythrocytic parasitesNeils B Quashie
Institute of Biomedical and Life Sciences, Division of Infection and Immunity, Glasgow G12 8TA, Scotland, UK
Biochem J 411:287-95. 2008..We thus propose a model for purine salvage in P. falciparum, based on the highly efficient uptake of hypoxanthine by PfNT1 and a high capacity for purine nucleoside uptake by a lower affinity carrier...
- Functional analysis of protein kinase CK2 of the human malaria parasite Plasmodium falciparumZoe Holland
INSERM U609, Wellcome Centre for Molecular Parasitology, Glasgow Biomedical Research Centre, University of Glasgow, Glasgow G12 8TA, Scotland, United Kingdom
Eukaryot Cell 8:388-97. 2009..falciparum CK2alpha enzymes to a small molecule inhibitor. Taken together, our data identify PfCK2alpha as a potential target for antimalarial chemotherapeutic intervention...
- Cyclin-dependent kinase homologues of Plasmodium falciparumChristian Doerig
INSERM U511 team, Wellcome Centre for Molecular Parasitology, The Anderson College, Glasgow, Scotland, UK
Int J Parasitol 32:1575-85. 2002..This review focuses on the current state-of-knowledge of Plasmodium falciparum cyclin-dependent kinase-like kinases and their regulators...
- The genes encoding cAMP-dependent protein kinase catalytic subunit homologues of the microsporidia Encephalitozoon intestinalis and E. cuniculi: molecular characterisation and phylogenetic analysisLeila Equinet
INSERM U609, Wellcome Centre for Molecular Parasitology, University of Glasgow, 56 Dumbarton Road, Glasgow G11 6NU, Scotland, UK
Parasitol Int 53:277-85. 2004..cuniculi orthologue (EcPKAc) is a single copy gene. RT-PCR data showed that the EiPKAc gene is expressed in at least one of the intracellular stages during infection of the mammalian host cell by E. intestinalis...
- The complement of protein kinases of the microsporidium Encephalitozoon cuniculi in relation to those of Saccharomyces cerevisiae and Schizosaccharomyces pombeDiego Miranda-Saavedra
College of Life Sciences, University of Dundee, Dow St, Dundee DD1 5EH, Scotland, UK
BMC Genomics 8:309. 2007..cuniculi genomic database aimed at identifying and classifying all protein kinases of this organism with reference to the kinomes of two highly-divergent yeast species, Saccharomyces cerevisiae and Schizosaccharomyces pombe...
- The protein-phosphatome of the human malaria parasite Plasmodium falciparumJonathan M Wilkes
INSERM U609, Wellcome Centre for Molecular Parasitology, University of Glasgow, Glasgow, Scotland, UK
BMC Genomics 9:412. 2008..We report an exhaustive analysis of the P. falciparum genomic database (PlasmoDB) aimed at identifying and classifying all protein phosphatases (PP) in this organism...
- Structures of P. falciparum protein kinase 7 identify an activation motif and leads for inhibitor designAnais Merckx
The Laboratory of Molecular Biophysics, Department of Biochemistry, University of Oxford, South Parks Road, Oxford OX1 3QU, United Kingdom
Structure 16:228-38. 2008..We identify two series of PFPK7 ATP-competitive inhibitors and suggest further developments for the design of selective and potent PFPK7 lead compounds as potential antimalarials...
- Drugging the Plasmodium kinome: the benefits of academia-industry synergyDidier Leroy
Merck Serono International S A, Geneva Research Center, 9, Chemin des Mines, Case postale 54, CH 1211 Genève 20, Switzerland
Trends Pharmacol Sci 29:241-9. 2008..Early characterisation of distribution, metabolism, pharmacokinetic (DMPK) and toxicology parameters are considered as well...
- Alisiaquinones and alisiaquinol, dual inhibitors of Plasmodium falciparum enzyme targets from a New Caledonian deep water spongeDenis Desoubzdanne
UMS Pierre Fabre CNRS 2587 and 2646, Institut de Sciences et Technologies du Medicament de Toulouse, 31432 Toulouse, France
J Nat Prod 71:1189-92. 2008..Alisiaquinone C displayed a submicromolar activity on P. falciparum and a competitive selectivity index on the different plasmodial strains...
- Antimalarial potential of xestoquinone, a protein kinase inhibitor isolated from a Vanuatu marine sponge Xestospongia spDominique Laurent
UMR152 IRD Université Paul Sabatier Toulouse III, Pharmacochimie des Substances Naturelles et Pharmacophores Redox, Centre IRD, BPA5, Noumea, New Caledonia
Bioorg Med Chem 14:4477-82. 2006..falciparum strain with an IC(50) of 3 microM and a weak selectivity index (SI 7). Xestoquinone exhibited a weak in vivo activity at 5mg/kg in Plasmodium berghei NK65 infected mice and was toxic at higher doses...
- Protein kinases as drug targets in parasitic protozoaChristian Doerig
Institut National de la Sante et de la Recherche Medicale, U 511, CHU Pitie Salpetriere, 91 Bd de l Hopital, 75013 Paris, France
Trends Parasitol 18:366-71. 2002..In addition, screening chemical libraries with active recombinant protein kinases can identify lead compounds for drug design...
- Structures of P. falciparum PfPK5 test the CDK regulation paradigm and suggest mechanisms of small molecule inhibitionSimon Holton
Laboratory of Molecular Biophysics, Department of Biochemistry, South Parks Road, OX1 3QU, Oxford, United Kingdom
Structure 11:1329-37. 2003..Such compounds will be useful tools for P. falciparum cell cycle studies, and will provide lead compounds for antimalarial drug development...
- Plasmodium falciparum glycogen synthase kinase-3: molecular model, expression, intracellular localisation and selective inhibitorsEliane Droucheau
C N R S, Cell Cycle Group, Station Biologique, B P 74, 29682 Roscoff Cedex, Bretagne, France
Biochim Biophys Acta 1697:181-96. 2004..A series of GSK-3 beta inhibitors were tested on both PfGSK-3 and mammalian GSK-3beta. Remarkably these enzymes show a partially divergent sensitivity to the compounds, suggesting that PfGSK-3 selective compounds might be identified...
- A novel protein kinase family in Plasmodium falciparum is differentially transcribed and secreted to various cellular compartments of the host cellMarta C Nunes
Unité de Biologie des Interactions Hôte Parasite, Institut Pasteur, CNRS, 75724 Paris, France
Mol Microbiol 63:391-403. 2007..Our data suggest a role of FIKK proteins in the remodelling of the erythrocyte surface and reveal the existence of an adaptable parasite system able to sense intra- and possibly extracellular changes...
- A stretch-activated anion channel is up-regulated by the malaria parasite Plasmodium falciparumStéphane Egée
Centre National de la Recherche Scientifique, UPR 9042, Station Biologique, Place G Teissier, B P 74, 29682 Roscoff Cedex, France
J Physiol 542:795-801. 2002..The data are consistent with the presence of two endogenous anion channels in human RBCs, of which one (the linear conductance channel) is up-regulated by the malaria parasite P. falciparum...
- A Plasmodium falciparum homologue of the Ran binding protein 1, a protein involved in nucleocytoplasmic transportVandana Ramachandran
Department of Biological Sciences, National University of Singapore, Science Drive 4, Singapore 119764, Singapore
Mol Biochem Parasitol 123:67-71. 2002
- A mitogen-activated protein kinase regulates male gametogenesis and transmission of the malaria parasite Plasmodium bergheiRadha Rangarajan
Department of Immunology and Infectious Diseases, Harvard School of Public Health, 665 Huntington Avenue, Boston, Massachusetts 02115, USA
EMBO Rep 6:464-9. 2005..Consistent with this result, transmission of mutant parasites to mosquitoes is grossly impaired. This finding identifies a crucial role for a MAPK pathway in malaria transmission...
- Pre-replication complex organization in the atypical DNA replication cycle of Plasmodium falciparum: characterization of the mini-chromosome maintenance (MCM) complex formationShelley Patterson
Department of Molecular Biology and Microbiology, University of Central Florida, Orlando, FL 32826, USA
Mol Biochem Parasitol 145:50-9. 2006..No phosphorylation of PfMCM2, 6 and 7 was detected, but two as yet unidentified threonine-phosphosphorylated proteins were present in the complex, whose pattern of phosphorylation varied during parasite development...
- An atypical mitogen-activated protein kinase controls cytokinesis and flagellar motility during male gamete formation in a malaria parasiteRita Tewari
Department of Biological Sciences, Imperial College London, London SW7 2AZ, UK
Mol Microbiol 58:1253-63. 2005....
- Regulation of protein phosphatase type 1 and cell cycle progression by PfLRR1, a novel leucine-rich repeat protein of the human malaria parasite Plasmodium falciparumWassim Daher
Unité Inserm 547 IPL, Institut Pasteur, 1 rue du Pr Calmette, B P 245, 59019 Lille Cedex, France
Mol Microbiol 60:578-90. 2006..Taken together, these observations suggest that PfLRR1 can regulate the cell cycle by binding to PP1 and regulating its activity...
- In vivo imaging enters parasitologyVolker Heussler
Bernhard Nocht Institute for Tropical Medicine, Department of Molecular Parasitology, Malaria laboratory I, Bernhard Nocht Strasse 74, 20359 Hamburg, Germany
Trends Parasitol 22:192-5; discussion 195-6. 2006..This rapidly developing field will go a long way towards deepening our understanding of host-parasite interactions at different levels...