Shareen H Doak

Summary

Affiliation: University of Wales
Country: UK

Publications

  1. doi request reprint In vitro genotoxicity testing strategy for nanomaterials and the adaptation of current OECD guidelines
    S H Doak
    Institute of Life Science, College of Medicine, Swansea University, Singleton Park, Swansea SA2 8PP, Wales, UK
    Mutat Res 745:104-11. 2012
  2. doi request reprint Confounding experimental considerations in nanogenotoxicology
    S H Doak
    Institute of Life Science, Swansea University, Wales, UK
    Mutagenesis 24:285-93. 2009
  3. doi request reprint No-observed effect levels are associated with up-regulation of MGMT following MMS exposure
    Shareen H Doak
    Institute of Life Science, School of Medicine, Swansea University, Singleton Park, Swansea, Wales, UK
    Mutat Res 648:9-14. 2008
  4. doi request reprint High-resolution imaging using a novel atomic force microscope and confocal laser scanning microscope hybrid instrument: essential sample preparation aspects
    Shareen H Doak
    School of Medicine, Institute of Life Science, Swansea University, Singleton Park, Swansea, SA2 8PP, Wales, UK
    Histochem Cell Biol 130:909-16. 2008
  5. ncbi request reprint Aneuploidy in upper gastro-intestinal tract cancers--a potential prognostic marker?
    Shareen H Doak
    Institute of Life Science, School of Medicine, Swansea University, Singleton Park, Swansea SA2 8PP, Wales, UK
    Mutat Res 651:93-104. 2008
  6. ncbi request reprint Bone morphogenic factor gene dosage abnormalities in prostatic intraepithelial neoplasia and prostate cancer
    Shareen H Doak
    School of Medicine, University of Wales Swansea, Singleton Park, Swansea SA2 8PP, Wales, UK
    Cancer Genet Cytogenet 176:161-5. 2007
  7. ncbi request reprint Mechanistic influences for mutation induction curves after exposure to DNA-reactive carcinogens
    Shareen H Doak
    School of Medicine, University of Wales Swansea, Singleton Park, Swansea, Wales, United Kingdom
    Cancer Res 67:3904-11. 2007
  8. ncbi request reprint Generation of locus-specific probes for interphase fluorescence in situ hybridisation--application in Barrett's esophagus
    S H Doak
    Human Molecular Pathology Group, School of Biological Sciences, University of Wales Swansea, Swansea, SA2 8PP, UK
    Exp Mol Pathol 77:26-33. 2004
  9. ncbi request reprint Differential expression of the MAD2, BUB1 and HSP27 genes in Barrett's oesophagus-their association with aneuploidy and neoplastic progression
    Shareen H Doak
    Human Molecular Pathology Group, School of Biological Sciences, University of Wales Swansea, Singleton Park, Swansea SA2 8PP, UK
    Mutat Res 547:133-44. 2004
  10. doi request reprint Real-time reverse-transcription polymerase chain reaction: technical considerations for gene expression analysis
    Shareen H Doak
    DNA Damage Research Group, Institute of Life Science, College of Medicine, Swansea University, Swansea, Wales, UK
    Methods Mol Biol 817:251-70. 2012

Collaborators

  • George E Johnson
  • G J Jenkins
  • Neenu Singh
  • James M Parry
  • Claire Morgan
  • Bryant C Nelson
  • Bernd Kaina
  • Murali Varma
  • Kelly Ann D Walker
  • Karen Brown
  • Emma L Quick
  • David O F Skibinski
  • Bella B Manshian
  • Sioned M Griffiths
  • Paul D Lewis
  • Helen Whiteland
  • Adam D Thomas
  • Anna L Seager
  • Andrew R Barron
  • Paul M Williams
  • Zoulikha M Zaïr
  • Peter R Dunstan
  • Timson C Appanna
  • Richard M Baylis
  • Jeanette Croft
  • Owen G Bodger
  • Pradeep Bose
  • Spencer Jenkins
  • Samantha Spencer-Harty
  • Rob Rickman
  • David Hywel Thomas
  • Howard Kynaston
  • Nicole Hondow
  • Neil Fenn
  • Owen Bodger
  • Chris Wright
  • Ken Brady
  • Christine Davies
  • Andrew P Brown
  • Karl Heinz Tomaszowski
  • Bryce J Marquis
  • Ume Kulsoom Shah
  • Andrew D Scott
  • Jane M Mikhail
  • Paul L Carmichael
  • Sharon J Scott
  • Chris J Wright
  • Raj Singh
  • Alvin W Orbaek
  • Mark D Holton
  • Spencer A Jenkins
  • Timothy P Stephenson
  • Howard G Kynaston
  • Tim H Brown
  • John N Baxter
  • A Paul Griffiths
  • Elizabeth M Parry

Detail Information

Publications33

  1. doi request reprint In vitro genotoxicity testing strategy for nanomaterials and the adaptation of current OECD guidelines
    S H Doak
    Institute of Life Science, College of Medicine, Swansea University, Singleton Park, Swansea SA2 8PP, Wales, UK
    Mutat Res 745:104-11. 2012
    ....
  2. doi request reprint Confounding experimental considerations in nanogenotoxicology
    S H Doak
    Institute of Life Science, Swansea University, Wales, UK
    Mutagenesis 24:285-93. 2009
    ..Thus, highlighting some of the potential confounding factors that need to be considered in order to ensure that in vitro genotoxicity assays report true biological impacts in response to nanomaterial exposure...
  3. doi request reprint No-observed effect levels are associated with up-regulation of MGMT following MMS exposure
    Shareen H Doak
    Institute of Life Science, School of Medicine, Swansea University, Singleton Park, Swansea, Wales, UK
    Mutat Res 648:9-14. 2008
    ....
  4. doi request reprint High-resolution imaging using a novel atomic force microscope and confocal laser scanning microscope hybrid instrument: essential sample preparation aspects
    Shareen H Doak
    School of Medicine, Institute of Life Science, Swansea University, Singleton Park, Swansea, SA2 8PP, Wales, UK
    Histochem Cell Biol 130:909-16. 2008
    ....
  5. ncbi request reprint Aneuploidy in upper gastro-intestinal tract cancers--a potential prognostic marker?
    Shareen H Doak
    Institute of Life Science, School of Medicine, Swansea University, Singleton Park, Swansea SA2 8PP, Wales, UK
    Mutat Res 651:93-104. 2008
    ....
  6. ncbi request reprint Bone morphogenic factor gene dosage abnormalities in prostatic intraepithelial neoplasia and prostate cancer
    Shareen H Doak
    School of Medicine, University of Wales Swansea, Singleton Park, Swansea SA2 8PP, Wales, UK
    Cancer Genet Cytogenet 176:161-5. 2007
    ..Approximately half of the prostate tumors displayed increased copy numbers of the BMP2, BMP5, BMP7, and UC28 gene loci, which may account for their abnormal gene expression patterns in neoplastic prostate tissue...
  7. ncbi request reprint Mechanistic influences for mutation induction curves after exposure to DNA-reactive carcinogens
    Shareen H Doak
    School of Medicine, University of Wales Swansea, Singleton Park, Swansea, Wales, United Kingdom
    Cancer Res 67:3904-11. 2007
    ..A pragmatic threshold for carcinogenicity may therefore exist for such genotoxins...
  8. ncbi request reprint Generation of locus-specific probes for interphase fluorescence in situ hybridisation--application in Barrett's esophagus
    S H Doak
    Human Molecular Pathology Group, School of Biological Sciences, University of Wales Swansea, Swansea, SA2 8PP, UK
    Exp Mol Pathol 77:26-33. 2004
    ..No significant alterations at the DNMT1 and DNMT3a loci were detected. An increased copy number of these genes is therefore not the basis for the hypermethylation commonly observed in this premalignant lesion...
  9. ncbi request reprint Differential expression of the MAD2, BUB1 and HSP27 genes in Barrett's oesophagus-their association with aneuploidy and neoplastic progression
    Shareen H Doak
    Human Molecular Pathology Group, School of Biological Sciences, University of Wales Swansea, Singleton Park, Swansea SA2 8PP, UK
    Mutat Res 547:133-44. 2004
    ..HSP27 transcriptional patterns therefore present potential as a prognostic tool to predict the aggressiveness of oesophageal adenocarcinomas (OA)...
  10. doi request reprint Real-time reverse-transcription polymerase chain reaction: technical considerations for gene expression analysis
    Shareen H Doak
    DNA Damage Research Group, Institute of Life Science, College of Medicine, Swansea University, Swansea, Wales, UK
    Methods Mol Biol 817:251-70. 2012
    ....
  11. pmc Influence of DNA repair on nonlinear dose-responses for mutation
    Adam D Thomas
    Institute of Life Science, College of Medicine, Swansea University, SA2 8PP, UK
    Toxicol Sci 132:87-95. 2013
    ..Inactivation of MGMT sensitizes cells to MNU-induced mutagenesis and shifts the NOGEL to the left on the dose axis...
  12. doi request reprint Single-walled carbon nanotubes: differential genotoxic potential associated with physico-chemical properties
    Bella B Manshian
    Institute of Life Science, School of Medicine, Swansea University, Singleton Park, Swansea, UK
    Nanotoxicology 7:144-56. 2013
    ..Consequently, this study has demonstrated that SWCNT genotoxicity is dependent on its secondary structure under experimental conditions and oxidative stress alone cannot account for the observed damage...
  13. pmc Pro-oxidant induced DNA damage in human lymphoblastoid cells: homeostatic mechanisms of genotoxic tolerance
    Anna L Seager
    DNA Damage Research Group, Institute of Life Science, College of Medicine, Swansea University, SA2 8PP, UK
    Toxicol Sci 128:387-97. 2012
    ..Hence, observed pro- oxidant thresholds were due to protective capacities of base excision repair enzymes and antioxidants against DNA damage, highlighting the importance of homeostatic mechanisms in "genotoxic tolerance."..
  14. pmc Dextran coated ultrafine superparamagnetic iron oxide nanoparticles: compatibility with common fluorometric and colorimetric dyes
    Sioned M Griffiths
    Institute of Life Science, School of Medicine, Swansea University, Singleton Park, Swansea SA2 8PP, Wales, UK
    Anal Chem 83:3778-85. 2011
    ....
  15. ncbi request reprint Analysis of the premalignant stages of Barrett's oesophagus through to adenocarcinoma by comparative genomic hybridization
    Jeanette Croft
    Human Molecular Pathology Group, Swansea Clinical School, University of Wales, UK
    Eur J Gastroenterol Hepatol 14:1179-86. 2002
    ..Our aim was to characterize karyotypic changes that may occur during the progression of Barrett's metaplasia through low-grade dysplasia and high-grade dysplasia to adenocarcinoma...
  16. doi request reprint The role of iron redox state in the genotoxicity of ultrafine superparamagnetic iron oxide nanoparticles
    Neenu Singh
    Institute of Life Science, College of Medicine, Swansea University, Singleton Park, Swansea SA2 8PP, Wales, UK
    Biomaterials 33:163-70. 2012
    ..As human exposure to ferrofluids is predicted to increase through nanomedicine based therapeutics, these findings are important in guiding the fabrication of USPION to ensure they have characteristics that support biocompatibility...
  17. doi request reprint N-methylpurine DNA glycosylase plays a pivotal role in the threshold response of ethyl methanesulfonate-induced chromosome damage
    Zoulikha M Zaïr
    Institute of Life Science, Swansea University, Swansea, SA2 8PP, UK
    Toxicol Sci 119:346-58. 2011
    ....
  18. doi request reprint NanoGenotoxicology: the DNA damaging potential of engineered nanomaterials
    Neenu Singh
    Institute of Life Science, School of Medicine, Swansea University, Singleton Park, Swansea SA2 8PP, Wales, UK
    Biomaterials 30:3891-914. 2009
    ..Hence, areas that require that further attention are highlighted and recommendations to improve our understanding of the genotoxic potential of engineered nanomaterials are addressed...
  19. doi request reprint Genotoxic thresholds, DNA repair, and susceptibility in human populations
    Gareth J S Jenkins
    Institute of Life Science, Swansea School of Medicine, Swansea University, Singleton Park, Swansea SA28PP, United Kingdom
    Toxicology 278:305-10. 2010
    ..By studying polymorphisms in DNA repair genes, susceptible individuals may be identified, and additional safety factors appropriately targeted to these populations...
  20. doi request reprint Non-linear dose-response of DNA-reactive genotoxins: recommendations for data analysis
    George E Johnson
    DNA Damage Group, School of Medicine, Swansea University, Wales, UK
    Mutat Res 678:95-100. 2009
    ..In this report, we will discuss the approaches that were taken to investigate potential threshold dose-response curves for DNA-reactive genotoxic compounds, with recommendations for further studies...
  21. pmc Characterisation of p53 status at the gene, chromosomal and protein levels in oesophageal adenocarcinoma
    S H Doak
    Human Molecular Pathology Group, School of Biological Sciences, University of Wales, Swansea SA2 8PP, UK
    Br J Cancer 89:1729-35. 2003
    ..P53 gene mutations are not the primary cause of protein overexpression--an alternative mechanism is responsible for the positive p53 immunohistochemistry detected...
  22. ncbi request reprint Genetic pathways involved in the progression of Barrett's metaplasia to adenocarcinoma
    G J S Jenkins
    Human Molecular Pathology Group, Swansea Clinical School, University of Wales Swansea, UK
    Br J Surg 89:824-37. 2002
    ..The prediction of which patients with Barrett's metaplasia will develop cancer is difficult. Better genetic characterization of the condition may aid clinicians in devising more effective management and follow-up strategies...
  23. pmc Chromosome 4 hyperploidy represents an early genetic aberration in premalignant Barrett's oesophagus
    S H Doak
    Human Molecular Pathology Group, School of Biological Sciences, University of Wales, Swansea SA2 8PP, UK
    Gut 52:623-8. 2003
    ..In an attempt to understand these causative pathways, fluorescence in situ hybridisation (FISH) was used in this study to determine when specific genetic alterations arise during Barrett's associated neoplastic progression...
  24. pmc Early p53 mutations in nondysplastic Barrett's tissue detected by the restriction site mutation (RSM) methodology
    G J S Jenkins
    Swansea Clinical School, University of Wales Swansea, UK
    Br J Cancer 88:1271-6. 2003
    ..4) and low-grade dysplasia patients (P=0.33) and 45% of high-grade dysplasia patients (P=0.15). Detected p53 mutations were mainly GC to AT transitions at CpG sites...
  25. ncbi request reprint The bile acid deoxycholic acid (DCA) at neutral pH activates NF-kappaB and induces IL-8 expression in oesophageal cells in vitro
    G J S Jenkins
    Swansea Clinical School, University of Wales Swansea, Swansea, Wales, UK
    Carcinogenesis 25:317-23. 2004
    ..Hence chromosome 4 amplification may provide a survival mechanism for bile exposed oesophageal tissues via NF-kappaB...
  26. doi request reprint The in vitro micronucleus assay and kinetochore staining: methodology and criteria for the accurate assessment of genotoxicity and cytotoxicity
    Bella B Manshian
    Institute of Life Science, College of Medicine, Swansea University, Swansea, Wales, UK
    Methods Mol Biol 1044:269-89. 2013
    ....
  27. pmc The role of adhesion molecules as biomarkers for the aggressive prostate cancer phenotype
    Claire Morgan
    Cancer Biomarkers Group, Institute of Life Science, College of Medicine, Swansea University, Swansea, United Kingdom
    PLoS ONE 8:e81666. 2013
    ..Alterations in key adherens (AJ) and tight junction (TJ) components have been hailed as potential biomarkers for prostate cancer progression but the majority of research has been carried out on individual molecules...
  28. doi request reprint Putative prognostic epithelial-to-mesenchymal transition biomarkers for aggressive prostate cancer
    Helen Whiteland
    College of Medicine, Swansea University, Swansea SA2 8PP, Wales, UK
    Exp Mol Pathol 95:220-6. 2013
    ..In addition, loss of E-Cadherin expression at the cellular membrane of prostate cancer cells was also significantly associated with increasing Gleason score, clinical stage, and additionally, a reduction in survival. ..
  29. pmc Quantum dots for multiplexed detection and characterisation of prostate cancer cells using a scanning near-field optical microscope
    Kelly Ann D Walker
    College of Engineering, Multidisciplinary Nanotechnology Centre, Swansea University, Singleton Park, Swansea, United Kingdom
    PLoS ONE 7:e31592. 2012
    ....
  30. doi request reprint Vinblastine and diethylstilboestrol tested in the in vitro mammalian cell micronucleus test (MNvit) at Swansea University UK in support of OECD draft Test Guideline 487
    George E Johnson
    DNA Damage Group, Institute of Life Science, School of Medicine, Swansea University, Swansea, United Kingdom
    Mutat Res 702:189-92. 2010
    ..In the cytokinesis blocked micronucleus assay, replicative index and cytokinesis block proliferation index were suitable cell death and cytostasis measures...
  31. ncbi request reprint Fluorescence imaging and investigations of directly labelled chromosomes using scanning near-field optical microscopy
    Richard M Baylis
    Multidisciplinary Nanotechnology Centre, Department of Physics, Swansea University, Singleton Park, Swansea SA2 8PP, UK
    Ultramicroscopy 107:308-12. 2007
    ..The results are discussed in the context of developing locus specific direct tags together with high resolution SNOM imaging for the observation of chromosome aberrations...
  32. ncbi request reprint Do dose response thresholds exist for genotoxic alkylating agents?
    G J S Jenkins
    Swansea School of Medicine, Swansea University, Singleton Park, Swansea SA2 8PP and School of Biological Sciences, University of Wales, Swansea, UK
    Mutagenesis 20:389-98. 2005
    ..Finally, genotoxic thresholds are currently being described for acute exposures to single agents in vitro, however, dose response data for chronic exposures to complex mixtures are, as yet, a long way off...
  33. ncbi request reprint Comparative genomic hybridization (CGH) of augmentation cystoplasties
    Timson C Appanna
    Department of Urology, University Hospital of Wales, Cardiff, UK
    Int J Urol 14:539-44. 2007
    ....